Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,215,450	T→C	Q423R (CAG→CGG)	aer ←	Aer

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,215,450	0	T	C	99.5%	591.7 / NA	189	Q423R (CAG→CGG)	aer	Aer
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (80/108); total (81/108)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 209 total aligned reads displayed.

GTTGAATGGCTAATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCTGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGC  >  NC_000913/3215360‑3215539

gTTGAATGGCTAATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCtgccgc                                                                                         >  1:1571391/1‑93 (MQ=255)

gTTGAATGGCTAATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCtgccgc                                                                                         <  1:2352547/93‑1 (MQ=255)

gTTGAATGGCTAATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCtgccgc                                                                                         >  1:1825982/1‑93 (MQ=255)

gTTGAATGGCTAATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCtgccgc                                                                                         <  1:1618593/93‑1 (MQ=255)

gTTGAATGGCTAATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCtgccgc                                                                                         >  1:392279/1‑93 (MQ=255)

    aaTGGCTAGTCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAAc                                                                                     <  2:3880161/93‑1 (MQ=255)

      tGGCTAATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCg                                                                                   <  1:4311443/93‑1 (MQ=255)

        gCTAATCTGTGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGa                                                                                 <  2:526754/93‑1 (MQ=255)

        gCTAATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGa                                                                                 <  2:4588046/93‑1 (MQ=255)

        gCTAATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGa                                                                                 >  2:3326152/1‑93 (MQ=255)

        gCTAATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGa                                                                                 <  1:2955917/93‑1 (MQ=255)

         cTAATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGAt                                                                                <  1:314900/93‑1 (MQ=255)

         cTAATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGAt                                                                                <  1:3803357/93‑1 (MQ=255)

          tAATCTGGGCGATCAACTGGGTGACGTTTTTCCCCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGAtt                                                                               <  1:1145835/93‑1 (MQ=255)

          tAATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGAtt                                                                               >  1:1861028/1‑93 (MQ=255)

          tAATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGAtt                                                                               >  2:299338/1‑93 (MQ=255)

          tAATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGCCCGGCGGCGTGTACCTGCCGCGAACCGGAtt                                                                               <  2:2007288/93‑1 (MQ=255)

            aTCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGc                                                                             <  1:4652346/93‑1 (MQ=255)

            aTCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGc                                                                             >  1:2072448/1‑93 (MQ=255)

             tCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCa                                                                            <  1:1713690/93‑1 (MQ=255)

              cTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCCGATTGCAc                                                                           >  2:1923693/1‑93 (MQ=255)

                gggCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCt                                                                         <  1:3596121/93‑1 (MQ=255)

                 ggCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCtt                                                                        >  2:2589368/1‑93 (MQ=255)

                   cGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTAt                                                                      <  1:469208/93‑1 (MQ=255)

                    gATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATc                                                                     <  1:58512/93‑1 (MQ=255)

                    gATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCACCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATc                                                                     <  2:1902248/93‑1 (MQ=255)

                     aTCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCa                                                                    >  1:965535/1‑93 (MQ=255)

                       cAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGc                                                                  >  2:1097703/1‑93 (MQ=255)

                        aaCTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTCCCTGCCGCGAACCGGATTGCACCTTATCAGCa                                                                 <  2:2056512/93‑1 (MQ=255)

                        aaCTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCa                                                                 >  1:1008845/1‑93 (MQ=255)

                         aCTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCAc                                                                >  1:2743108/1‑93 (MQ=255)

                         aCTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCAc                                                                >  1:1107089/1‑93 (MQ=255)

                          cTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACt                                                               <  1:3247271/93‑1 (MQ=255)

                           tGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATTAGCACTg                                                              >  2:3392891/1‑93 (MQ=255)

                           tGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTg                                                              >  1:4114586/1‑93 (MQ=255)

                            gggTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTgg                                                             >  1:2227725/1‑93 (MQ=255)

                              gTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCa                                                           <  1:4389572/93‑1 (MQ=255)

                                gACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCTGCGAACCGGATTGCACCTTATCAGTACTGCCatc                                                         >  1:4012974/1‑93 (MQ=255)

                                gACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCatc                                                         >  1:1065941/1‑93 (MQ=255)

                                 aCGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCatca                                                        >  2:3705656/1‑93 (MQ=255)

                                 aCGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCatca                                                        <  1:4141299/93‑1 (MQ=255)

                                  cGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCatcaa                                                       >  2:731820/1‑93 (MQ=255)

                                    tttttCCCCTGTGCCACAATATCTTCCATCGTCCGCCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCatcaatc                                                     <  2:201979/93‑1 (MQ=255)

                                    tttttCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCatcaatc                                                     >  2:4113169/1‑93 (MQ=255)

                                    tttttCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCatcaatc                                                     <  1:4474351/93‑1 (MQ=255)

                                    tttttCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCatcaatc                                                     >  2:3175939/1‑93 (MQ=255)

                                     ttttCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCatcaatca                                                    <  2:1393027/93‑1 (MQ=255)

                                     ttttCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCatcaatca                                                    <  1:1301140/93‑1 (MQ=255)

                                       ttCTCCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGc                                                  <  1:2799560/93‑1 (MQ=255)

                                       ttCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGc                                                  <  2:2042592/93‑1 (MQ=255)

                                        tCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCt                                                 <  1:4613329/93‑1 (MQ=255)

                                         cgccTGTGCCAAAATATCATCCATCGTCCGTCGGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCtt                                                <  2:1198317/91‑1 (MQ=255)

                                          aCCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTg                                               >  2:2429444/1‑93 (MQ=255)

                                          aCCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTg                                               >  1:2858039/1‑93 (MQ=255)

                                            cTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCg                                             >  2:4022411/1‑93 (MQ=255)

                                             tgtgCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGa                                            >  1:2283749/1‑93 (MQ=255)

                                              gtgCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGaa                                           <  2:70431/93‑1 (MQ=255)

                                                 ccACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAatat                                        <  1:1212524/93‑1 (MQ=255)

                                                  cacaATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATc                                       >  2:1450628/1‑93 (MQ=255)

                                                  cacaATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATc                                       >  2:4468936/1‑93 (MQ=255)

                                                  cacaATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATc                                       <  1:2140501/93‑1 (MQ=255)

                                                  cacaATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATc                                       >  1:4358804/1‑93 (MQ=255)

                                                   acaATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCg                                      >  2:227991/1‑93 (MQ=255)

                                                    caATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGt                                     <  2:897230/93‑1 (MQ=255)

                                                    caATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGt                                     <  1:2943255/93‑1 (MQ=255)

                                                     aaTATTTTCCATCGTCCGTCCGGCGGCTTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGtt                                    <  1:3794093/93‑1 (MQ=255)

                                                     aaTATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGtt                                    >  1:355846/1‑93 (MQ=255)

                                                     aaTATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGtt                                    <  1:2982687/93‑1 (MQ=255)

                                                      atatCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTg                                   >  1:4456508/1‑93 (MQ=255)

                                                      atatCTTCCATCGTCCGTCCGGCGGCGTCTCCCTGCCGCGAACCGGATTGCCCCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTg                                   <  2:4342257/93‑1 (MQ=255)

                                                       tatCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTgg                                  >  1:98684/1‑93 (MQ=255)

                                                       tatCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTgg                                  <  1:2029115/93‑1 (MQ=255)

                                                       tatCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTgg                                  <  1:3161809/93‑1 (MQ=255)

                                                         tCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGgca                                <  2:1529178/93‑1 (MQ=255)

                                                            tCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGgcagca                             <  2:3517127/93‑1 (MQ=255)

                                                             ccATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCAt                            >  2:2336620/1‑93 (MQ=255)

                                                             ccATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTCCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCAt                            <  2:2666520/93‑1 (MQ=255)

                                                             cagtCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCAt                            <  1:2609487/90‑1 (MQ=255)

                                                               aTCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGCATATCGTTGGCAGCCTTa                          >  2:669679/1‑93 (MQ=255)

                                                                tCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAg                         <  1:423542/93‑1 (MQ=255)

                                                                ccgtccgtcCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAg                         <  2:1123285/92‑1 (MQ=255)

                                                                ccgcccgtccgGCGGCGTGTCCCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAg                         <  2:4015112/89‑1 (MQ=255)

                                                                 cgtccgtcCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAgc                        <  1:3058548/93‑1 (MQ=255)

                                                                 cgtccgtcCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAgc                        >  1:1230130/1‑93 (MQ=255)

                                                                 cgtccgtcCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAgc                        <  1:2507500/93‑1 (MQ=255)

                                                                 cgtccgtcCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAgc                        <  2:2888199/93‑1 (MQ=255)

                                                                  gtccgtccGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAgcg                       >  2:859827/1‑93 (MQ=255)

                                                                   tccgtccgGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAgcgc                      >  1:3383101/1‑93 (MQ=255)

                                                                   tccgtccgGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAgcgc                      >  1:3641698/1‑93 (MQ=255)

                                                                    ccgtccgGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCt                     <  2:2976405/93‑1 (MQ=255)

                                                                      gtccgGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTgg                   >  2:3809664/1‑92 (MQ=255)

                                                                      gtccgGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGc                   >  1:740299/1‑93 (MQ=255)

                                                                             ggcgTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCggctggc            >  2:4283879/1‑93 (MQ=255)

                                                                                 tgtACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTaaa        >  1:913295/1‑93 (MQ=255)

                                                                                   tACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATg      <  1:1166390/93‑1 (MQ=255)

                                                                                    aCCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGa     <  1:165959/93‑1 (MQ=255)

                                                                                     ccTGCCGCGAACCGGATTGCTCCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGAc    <  1:2317202/93‑1 (MQ=255)

                                                                                     ccTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGAc    <  2:3242199/93‑1 (MQ=255)

                                                                                       tgccgcGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGc  >  2:3921271/1‑93 (MQ=255)

                                                                                       tgccgcGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGc  <  2:606540/93‑1 (MQ=255)

GTTGAATGGCTAATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCTGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGC  >  NC_000913/3215360‑3215539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AGCGTTGAATGGCTAATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCTGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTT  >  NC_000913/3215357‑3215543

AGCGTTGAATGGCTAATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCC                                                                                               <  SRR4302535.4628827/94‑1 (MQ=60)

AGCGTTGAATGGCTAATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCC                                                                                               >  SRR4302535.2879147/1‑94 (MQ=60)

AGCGTTGAATGGCTAATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGGCCGTCCGGCGGCGTGTACCTGCC                                                                                               <  SRR4302535.1566328/94‑1 (MQ=60)

 GCGTTGAATGGCTAATCTGGGCGATCAACTGGGGGACGTTTTTCACCTGTGCCACACTATATTCCATCGTCCGTCCGGCGGCGTGTACCTGCCG                                                                                              >  SRR4302535.4510809/1‑94 (MQ=60)

  CGTTGAATGGCTAATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGC                                                                                             >  SRR4302535.395014/1‑94 (MQ=60)

  CGTTGAATGGCTAATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGC                                                                                             >  SRR4302535.1582734/1‑94 (MQ=60)

  CGTTGAATGGCTAATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGC                                                                                             >  SRR4302535.1280373/1‑94 (MQ=60)

  CGTTGAATGGCTAATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGC                                                                                             >  SRR4302535.1839163/1‑94 (MQ=60)

  CGTTGAATGGCTAATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGC                                                                                             >  SRR4302535.2725159/1‑94 (MQ=60)

   GTTGAATGGCTAATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCG                                                                                            <  SRR4302535.2369412/94‑1 (MQ=60)

   GTTGAATGGCTAATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCG                                                                                            >  SRR4302535.2724931/1‑94 (MQ=60)

   GTTGAATGGCTAATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCG                                                                                            >  SRR4302535.2456712/1‑94 (MQ=60)

   GTTGAATGGCTAATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCG                                                                                            <  SRR4302535.1630265/94‑1 (MQ=60)

     TGAATGGCTAATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAA                                                                                          <  SRR4302535.943532/94‑1 (MQ=60)

     TGAATGGCTAATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAA                                                                                          >  SRR4302535.450361/1‑94 (MQ=60)

        ATGGCTAATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCG                                                                                       <  SRR4302535.1575883/94‑1 (MQ=60)

         TGGCTAATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGG                                                                                      >  SRR4302535.1166345/1‑94 (MQ=60)

         TGGCTAATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGG                                                                                      <  SRR4302535.4340936/94‑1 (MQ=60)

           GCTAATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGAT                                                                                    <  SRR4302535.2976676/94‑1 (MQ=60)

            CTAATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATT                                                                                   >  SRR4302535.2180753/1‑94 (MQ=60)

            CTAATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATT                                                                                   >  SRR4302535.1874448/1‑94 (MQ=60)

            CTAATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATT                                                                                   <  SRR4302535.16714/94‑1 (MQ=60)

            CTAATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATT                                                                                   <  SRR4302535.3829576/94‑1 (MQ=60)

            CTAATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATT                                                                                   <  SRR4302535.2814242/94‑1 (MQ=60)

            CTAATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATT                                                                                   <  SRR4302535.317077/94‑1 (MQ=60)

             TAATCTGGGCGATCAACTGGGTGACGTTTTTCCCCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTG                                                                                  <  SRR4302535.1154001/94‑1 (MQ=60)

              AATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGC                                                                                 <  SRR4302535.1694994/94‑1 (MQ=60)

              AATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGC                                                                                 >  SRR4302535.3820389/1‑94 (MQ=60)

              AATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGC                                                                                 >  SRR4302535.2087380/1‑94 (MQ=60)

               ATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCA                                                                                <  SRR4302535.1116878/94‑1 (MQ=60)

               ATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCA                                                                                <  SRR4302535.4683847/94‑1 (MQ=60)

                TCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCAC                                                                               <  SRR4302535.1726082/94‑1 (MQ=60)

                 CTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACC                                                                              >  SRR4302535.2577739/1‑94 (MQ=60)

                  TGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCT                                                                             >  SRR4302535.3524683/1‑94 (MQ=60)

                   GGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTT                                                                            <  SRR4302535.3621077/94‑1 (MQ=60)

                      CGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATC                                                                         <  SRR4302535.472490/94‑1 (MQ=60)

                       GATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCA                                                                        >  SRR4302535.972399/1‑94 (MQ=60)

                       GATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCA                                                                        <  SRR4302535.58941/94‑1 (MQ=60)

                       GATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCA                                                                        >  SRR4302535.2972296/1‑94 (MQ=60)

                        ATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAG                                                                       <  SRR4302535.4550714/94‑1 (MQ=60)

                        ATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAG                                                                       <  SRR4302535.1825325/94‑1 (MQ=60)

                          CAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCA                                                                     >  SRR4302535.1016013/1‑94 (MQ=60)

                           AACTGGGTGACGTTTTTCCCCTGTGCCACAATATCTGCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCGCCTTATCAGCAC                                                                    <  SRR4302535.347561/94‑1 (MQ=60)

                           AACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCAC                                                                    >  SRR4302535.2762661/1‑94 (MQ=60)

                           AACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCAC                                                                    >  SRR4302535.1114998/1‑94 (MQ=60)

                           AACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCAC                                                                    <  SRR4302535.4392989/94‑1 (MQ=60)

                             CTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTG                                                                  <  SRR4302535.3269825/94‑1 (MQ=60)

                             CTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTG                                                                  >  SRR4302535.4142750/1‑94 (MQ=60)

                             CTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTG                                                                  <  SRR4302535.681554/94‑1 (MQ=60)

                             CTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTG                                                                  >  SRR4302535.981103/1‑94 (MQ=60)

                              TGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGG                                                                 >  SRR4302535.2243573/1‑94 (MQ=60)

                                 GTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCAT                                                              >  SRR4302535.3619739/1‑94 (MQ=60)

                                 GTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCAT                                                              <  SRR4302535.4419606/94‑1 (MQ=60)

                                  TGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCTGCGAACCGGATTGCACCTTATCAGTACTGCCATC                                                             >  SRR4302535.4040425/1‑94 (MQ=60)

                                  TGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATC                                                             >  SRR4302535.1073525/1‑94 (MQ=60)

                                    GCGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAA                                                           <  SRR4302535.435191/94‑1 (MQ=60)

                                    ACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAA                                                           <  SRR4302535.4169656/94‑1 (MQ=60)

                                    ACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAA                                                           <  SRR4302535.1040841/94‑1 (MQ=60)

                                     CGTTTTTCCCCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAAT                                                          <  SRR4302535.1976044/94‑1 (MQ=60)

                                     CGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAAT                                                          >  SRR4302535.282228/1‑94 (MQ=60)

                                     CGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAAT                                                          >  SRR4302535.990642/1‑94 (MQ=60)

                                      GTTTTTCACCTGTGGCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATC                                                         <  SRR4302535.3172358/94‑1 (MQ=60)

                                       TTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCA                                                        <  SRR4302535.4505052/94‑1 (MQ=60)

                                        TTTTCACCTGTGCCACAATATCTTCCATCGTCCGTGCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAG                                                       <  SRR4302535.3893942/94‑1 (MQ=60)

                                        TTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAG                                                       <  SRR4302535.1194313/94‑1 (MQ=60)

                                        TTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAG                                                       <  SRR4302535.1310418/94‑1 (MQ=60)

                                          TTCTCCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCT                                                     <  SRR4302535.2819478/94‑1 (MQ=60)

                                          TTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCT                                                     <  SRR4302535.2358093/94‑1 (MQ=60)

                                           TCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTT                                                    <  SRR4302535.3797656/94‑1 (MQ=60)

                                           TCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTT                                                    <  SRR4302535.4644671/94‑1 (MQ=60)

                                            CACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTG                                                   >  SRR4302535.2878289/1‑94 (MQ=60)

                                               CTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGA                                                >  SRR4302535.2300067/1‑94 (MQ=60)

                                                 GTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAAT                                              <  SRR4302535.401734/94‑1 (MQ=60)

                                                   GCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATAT                                            >  SRR4302535.1223178/1‑94 (MQ=60)

                                                    CCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATC                                           >  SRR4302535.4388643/1‑94 (MQ=60)

                                                    CCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATC                                           <  SRR4302535.1221222/94‑1 (MQ=60)

                                                     CACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCG                                          <  SRR4302535.473921/94‑1 (MQ=60)

                                                     CACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCG                                          <  SRR4302535.4645578/94‑1 (MQ=60)

                                                     CACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCG                                          <  SRR4302535.2155911/94‑1 (MQ=60)

                                                     CACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCG                                          >  SRR4302535.4148107/1‑94 (MQ=60)

                                                       CAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTT                                        <  SRR4302535.2963947/94‑1 (MQ=60)

                                                       CAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTT                                        >  SRR4302535.358301/1‑94 (MQ=60)

                                                        AATATTTTCCATCGTCCGTCCGGCGGCTTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTG                                       <  SRR4302535.3820249/94‑1 (MQ=60)

                                                        AATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTG                                       >  SRR4302535.4487072/1‑94 (MQ=60)

                                                        AATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTG                                       <  SRR4302535.3003608/94‑1 (MQ=60)

                                                        AATATCTTCCATCGTCCGCCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTG                                       <  SRR4302535.1853946/94‑1 (MQ=60)

                                                         ATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGG                                      >  SRR4302535.99381/1‑94 (MQ=60)

                                                         ATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGG                                      >  SRR4302535.842532/1‑94 (MQ=60)

                                                         ATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGG                                      >  SRR4302535.4693670/1‑94 (MQ=60)

                                                          TATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGC                                     <  SRR4302535.3183741/94‑1 (MQ=60)

                                                          TATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGC                                     <  SRR4302535.2043742/94‑1 (MQ=60)

                                                          TATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGC                                     <  SRR4302535.226033/94‑1 (MQ=60)

                                                            TCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAG                                   <  SRR4302535.3241906/94‑1 (MQ=60)

                                                             CTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGC                                  <  SRR4302535.1607806/94‑1 (MQ=60)

                                                             CTTCCATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGC                                  >  SRR4302535.2993966/1‑94 (MQ=60)

                                                                cagTCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATT                               <  SRR4302535.2628213/91‑1 (MQ=60)

                                                                  ATCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAG                             >  SRR4302535.4184709/1‑94 (MQ=60)

                                                                   TCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGC                            >  SRR4302535.1238923/1‑94 (MQ=60)

                                                                   TCGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGC                            <  SRR4302535.426497/94‑1 (MQ=60)

                                                                    CGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCG                           <  SRR4302535.2525577/94‑1 (MQ=60)

                                                                    CGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCG                           >  SRR4302535.2055746/1‑94 (MQ=60)

                                                                    CGTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCG                           <  SRR4302535.3079941/94‑1 (MQ=60)

                                                                     GTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGC                          >  SRR4302535.3406621/1‑94 (MQ=60)

                                                                     GTCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGC                          >  SRR4302535.3666922/1‑94 (MQ=60)

                                                                      TCCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCT                         <  SRR4302535.4484660/94‑1 (MQ=60)

                                                                       CCGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTG                        <  SRR4302535.4350363/94‑1 (MQ=60)

                                                                        CGTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGC                       >  SRR4302535.745501/1‑94 (MQ=60)

                                                                         GTGCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCG                      <  SRR4302535.2319228/94‑1 (MQ=60)

                                                                         GTCCGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCG                      <  SRR4302535.4337305/94‑1 (MQ=60)

                                                                            CGGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCT                   <  SRR4302535.3164381/94‑1 (MQ=60)

                                                                             GGCGGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTG                  >  SRR4302535.3270682/1‑94 (MQ=60)

                                                                                GGCGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCT               <  SRR4302535.4057006/94‑1 (MQ=60)

                                                                                  CGTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTAA             >  SRR4302535.2099739/1‑94 (MQ=60)

                                                                                   GTGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAA            >  SRR4302535.919786/1‑94 (MQ=60)

                                                                                    TGTACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAAT           <  SRR4302535.1090816/94‑1 (MQ=60)

                                                                                      TACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGA         <  SRR4302535.1174721/94‑1 (MQ=60)

                                                                                       ACCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGAC        <  SRR4302535.167115/94‑1 (MQ=60)

                                                                                        CCTGCCGCGAACCGGATTGCTCCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGACG       <  SRR4302535.2333784/94‑1 (MQ=60)

                                                                                        CCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGACG       <  SRR4302535.291625/94‑1 (MQ=60)

                                                                                        CCTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGACG       <  SRR4302535.3059553/94‑1 (MQ=60)

                                                                                         CTGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGC      <  SRR4302535.1807358/94‑1 (MQ=60)

                                                                                          TGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGCA     >  SRR4302535.4092359/1‑94 (MQ=60)

                                                                                          TGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGCA     >  SRR4302535.1283125/1‑94 (MQ=60)

                                                                                          TGCCGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGCA     <  SRR4302535.3657154/94‑1 (MQ=60)

                                                                                             CGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTT  <  SRR4302535.2869529/94‑1 (MQ=60)

AGCGTTGAATGGCTAATCTGGGCGATCAACTGGGTGACGTTTTTCACCTGTGCCACAATATCTTCCATCGTCCGTCCGGCGGCGTGTACCTGCTGCGAACCGGATTGCACCTTATCAGCACTGGCATCAATCAGCTTGCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTT  >  NC_000913/3215357‑3215543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`