Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,218,562	G→A	V109V (GTC→GTT)	ygjH ←	putative tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,218,562	0	G	A	97.1%	699.3 / 2.1	206	V109V (GTC→GTT)	ygjH	putative tRNA synthetase
Reads supporting (aligned to +/- strand): ref base G (4/2); new base A (116/84); total (120/86)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.67e-01

Only 100 of 222 total aligned reads displayed.

TAAGGCGCTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACGACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTC  >  NC_000913/3218472‑3218651

   ggCGCTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAAcgcg                                                                                      <  1:465945/93‑1 (MQ=255)

    gcgcTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGt                                                                                     <  2:4131654/93‑1 (MQ=255)

     ggcTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTa                                                                                    <  1:1063573/92‑1 (MQ=255)

         ggcggcCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGcc                                                                                >  1:2137189/1‑93 (MQ=255)

             gcCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCa                                                                            >  2:3883650/1‑93 (MQ=255)

             gcCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCa                                                                            >  1:823816/1‑93 (MQ=255)

              ccATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAg                                                                           <  2:4481103/93‑1 (MQ=255)

                    gAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGcatca                                                                     >  2:2094668/1‑93 (MQ=255)

                    gAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGcatca                                                                     >  2:839509/1‑93 (MQ=255)

                      gTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCatcatc                                                                   >  2:3486414/1‑93 (MQ=255)

                      gTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCatcatc                                                                   >  1:3751177/1‑93 (MQ=255)

                      gTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCCATCTTTTTTTACAACGCGTACGCCCCCAGGCatcatc                                                                   >  1:2402847/1‑93 (MQ=255)

                       tAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCGGGCATCATcc                                                                  >  2:2649196/1‑93 (MQ=255)

                       tAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATcc                                                                  <  2:1032843/93‑1 (MQ=255)

                         aGTGTCGACGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGc                                                                >  2:4570649/1‑93 (MQ=255)

                         aGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGc                                                                <  1:904778/93‑1 (MQ=255)

                             tCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCg                                                            >  1:4001812/1‑93 (MQ=255)

                               gaagaaGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCGCGCAGGCATCATCCGCTCCggg                                                          >  1:3723937/1‑92 (MQ=255)

                                aagaagCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCg                                                         >  1:2775100/1‑93 (MQ=255)

                                 agaagCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATTATCCGCCTCGGCGt                                                        >  2:4608485/1‑93 (MQ=255)

                                 agaagCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGt                                                        >  2:4090426/1‑93 (MQ=255)

                                 agaagCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGt                                                        >  1:3728728/1‑93 (MQ=255)

                                  gaagCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGtt                                                       >  2:1711836/1‑93 (MQ=255)

                                    agCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTaa                                                     <  2:1390651/93‑1 (MQ=255)

                                    agCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTaa                                                     >  1:1016108/1‑93 (MQ=255)

                                     gCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGGTAAc                                                    >  2:94127/1‑93 (MQ=255)

                                      cGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACa                                                   <  2:1974821/93‑1 (MQ=255)

                                      cGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACa                                                   >  2:1521936/1‑93 (MQ=255)

                                       gTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAg                                                  >  2:3709293/1‑93 (MQ=255)

                                        tAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGc                                                 >  2:3622005/1‑93 (MQ=255)

                                        tAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGc                                                 >  1:1848564/1‑93 (MQ=255)

                                        tAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGc                                                 >  1:761082/1‑93 (MQ=255)

                                        tAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATAAAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTCACCGc                                                 >  1:192109/1‑93 (MQ=255)

                                         aaTATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCa                                                <  1:3133360/93‑1 (MQ=255)

                                         aaTATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAAAGCGTACGCCCGCAGGCATCATACGCTCCGGCGTTTAAAGCa                                                >  1:3232288/1‑93 (MQ=255)

                                          atatCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCAc                                               >  2:3081833/1‑93 (MQ=255)

                                          atatCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCAc                                               <  1:3435666/93‑1 (MQ=255)

                                           tatCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACg                                              >  2:1677849/1‑93 (MQ=255)

                                            atCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGc                                             >  2:2971614/1‑93 (MQ=255)

                                             tCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCt                                            >  1:2115147/1‑93 (MQ=255)

                                               aaaGCGGAGGGCTATACGGCGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACTCttt                                          <  2:3517138/93‑1 (MQ=255)

                                                  acGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCAc                                       <  1:1581683/92‑1 (MQ=255)

                                                   cGGATGTCCATCCGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACt                                      <  1:2710548/93‑1 (MQ=255)

                                                   cGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACt                                      <  2:1299796/93‑1 (MQ=255)

                                                     gATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGc                                    <  1:3073149/93‑1 (MQ=255)

                                                     gATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGc                                    <  2:921213/93‑1 (MQ=255)

                                                      aTGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGcc                                   >  2:2817182/1‑93 (MQ=255)

                                                       tGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCg                                  >  2:2731615/1‑93 (MQ=255)

                                                       tGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCg                                  <  1:2954618/93‑1 (MQ=255)

                                                        ggCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGt                                 >  1:2363562/1‑93 (MQ=255)

                                                          cGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCa                               <  1:3306392/93‑1 (MQ=255)

                                                           gATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCAt                              >  1:3864427/1‑93 (MQ=255)

                                                            aTACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATc                             >  2:1378361/1‑93 (MQ=255)

                                                             tACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCg                            >  2:1850362/1‑93 (MQ=255)

                                                                gacgTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGtt                         >  1:2920281/1‑93 (MQ=255)

                                                                gacgTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGtt                         <  2:3217182/93‑1 (MQ=255)

                                                                 acgTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGttt                        <  1:358356/93‑1 (MQ=255)

                                                                  cgTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTc                       >  2:3304377/1‑93 (MQ=255)

                                                                  cgTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTc                       <  1:188602/93‑1 (MQ=255)

                                                                    tCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCg                     <  2:4547247/93‑1 (MQ=255)

                                                                     cGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGc                    <  1:205993/93‑1 (MQ=255)

                                                                      gCCATCCGATCTTTTTATACGACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGca                   >  2:1273923/1‑93 (MQ=255)

                                                                      gCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGca                   >  2:3729680/1‑93 (MQ=255)

                                                                       ccATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGcac                  <  1:1607112/93‑1 (MQ=255)

                                                                       ccATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGcac                  >  2:2332426/1‑93 (MQ=255)

                                                                         aTCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGcacac                >  2:2214085/1‑93 (MQ=255)

                                                                         aTCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGcacac                >  2:4438739/1‑93 (MQ=255)

                                                                         aTCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGcacac                >  1:1733476/1‑93 (MQ=255)

                                                                         aTCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGcacac                >  1:4110285/1‑93 (MQ=255)

                                                                          tCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGcacaca               <  2:192035/93‑1 (MQ=255)

                                                                            cGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACacaac             >  2:4226847/1‑93 (MQ=255)

                                                                            cGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACacaac             <  2:1789477/93‑1 (MQ=255)

                                                                            cGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACacaac             >  1:3535076/1‑93 (MQ=255)

                                                                            cGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACacaac             <  1:2974290/93‑1 (MQ=255)

                                                                             gATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACacaaca            >  2:747332/1‑93 (MQ=255)

                                                                             gATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACacaaca            >  1:4581703/1‑93 (MQ=255)

                                                                             gATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACacaaca            >  1:4440820/1‑93 (MQ=255)

                                                                             caTCTTTTTATCCAACGCGTCCGCCCGCAGGCATCATCCGCTCCGGCGTTAACGGCACCCTTCCACTGCCGTCATCGGTTTCCGCACacaaca            <  2:3693381/92‑1 (MQ=255)

                                                                              aTCTTTTTATACGACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAg           >  1:694579/1‑93 (MQ=255)

                                                                              aTCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTGACTGCCGTCATCGGTTTCCGCCCACAACAg           >  2:304082/1‑93 (MQ=255)

                                                                              aTCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAg           >  2:1690442/1‑93 (MQ=255)

                                                                              aTCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAg           >  1:3780059/1‑93 (MQ=255)

                                                                               tCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAgc          <  2:166747/93‑1 (MQ=255)

                                                                               tCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAgc          >  2:3984386/1‑93 (MQ=255)

                                                                                cTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAgca         >  1:639903/1‑93 (MQ=255)

                                                                                cTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAgca         >  2:2612897/1‑93 (MQ=255)

                                                                                 tttttATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAgcat        <  1:1179536/93‑1 (MQ=255)

                                                                                 tttttATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAgcat        <  1:4504945/93‑1 (MQ=255)

                                                                                 tttttATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAgcat        <  1:181987/93‑1 (MQ=255)

                                                                                 tttttATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAgcat        <  1:2206572/93‑1 (MQ=255)

                                                                                 tttttATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAgcat        >  2:1876063/1‑93 (MQ=255)

                                                                                  ttttATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAgcatg       <  2:2273175/93‑1 (MQ=255)

                                                                                    ttATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAgcatgca     <  2:4174311/93‑1 (MQ=255)

                                                                                    ttATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAgcatgca     <  1:1449791/93‑1 (MQ=255)

                                                                                    ttATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAgcatgca     >  2:2360668/1‑93 (MQ=255)

                                                                                     tataCAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCAGGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAgcatgcat    >  1:1113823/1‑93 (MQ=255)

                                                                                      ataCAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCAtt   <  1:2558094/93‑1 (MQ=255)

                                                                                       taCAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTc  <  1:1241595/93‑1 (MQ=255)

                                                                                       taCAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTc  <  2:1172610/93‑1 (MQ=255)

                                                                                       taCAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTc  >  2:2486670/1‑93 (MQ=255)

TAAGGCGCTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACGACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTC  >  NC_000913/3218472‑3218651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCGTAAGGCGCTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACGACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTCCGAC  >  NC_000913/3218469‑3218655

GCGTAAGGCGCTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACA                                                                                               <  SRR4302535.3171117/94‑1 (MQ=60)

 CGTAAGGCGCTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAA                                                                                              <  SRR4302535.2496467/94‑1 (MQ=60)

 CGTAAGGCGCTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAA                                                                                              >  SRR4302535.1530975/1‑94 (MQ=60)

  GTAAGGCGCTGGCGGCCATGGGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAAC                                                                                             <  SRR4302535.992715/94‑1 (MQ=60)

  GTAAGGCGCTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAAC                                                                                             <  SRR4302535.3246263/94‑1 (MQ=60)

    AAGGCGCTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGC                                                                                           >  SRR4302535.1853313/1‑94 (MQ=60)

      GGCGCTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGT                                                                                         <  SRR4302535.469206/94‑1 (MQ=60)

        GGCTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTAC                                                                                       <  SRR4302535.1071141/94‑1 (MQ=60)

        CGCTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTAC                                                                                       >  SRR4302535.388117/1‑94 (MQ=60)

        CGCTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTAC                                                                                       >  SRR4302535.3180434/1‑94 (MQ=60)

         GCTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACG                                                                                      <  SRR4302535.1176877/94‑1 (MQ=60)

           TGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCC                                                                                    >  SRR4302535.2152582/1‑94 (MQ=60)

               GGCCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCA                                                                                >  SRR4302535.829590/1‑94 (MQ=60)

                 CCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGG                                                                              >  SRR4302535.1633800/1‑94 (MQ=60)

                 CCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGG                                                                              <  SRR4302535.218660/94‑1 (MQ=60)

                       GAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCAT                                                                        >  SRR4302535.1789819/1‑94 (MQ=60)

                        AGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCTCCATCCGATCTTTTTATACAACGCGTACGGCCGCAGGCATCATC                                                                       >  SRR4302535.253731/1‑94 (MQ=60)

                        AGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATC                                                                       >  SRR4302535.3777143/1‑94 (MQ=60)

                        AGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATC                                                                       >  SRR4302535.2957321/1‑94 (MQ=60)

                        AGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCCATCTTTTTTTACAACGCGTACGCCCCCAGGCATCATC                                                                       >  SRR4302535.2420131/1‑94 (MQ=60)

                         GTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCC                                                                      <  SRR4302535.1089865/94‑1 (MQ=60)

                          TAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCG                                                                     >  SRR4302535.390392/1‑94 (MQ=60)

                          TAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCG                                                                     <  SRR4302535.3335554/94‑1 (MQ=60)

                            AGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCT                                                                   >  SRR4302535.770232/1‑94 (MQ=60)

                            AGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCT                                                                   >  SRR4302535.2558154/1‑94 (MQ=60)

                            AGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCT                                                                   <  SRR4302535.911199/94‑1 (MQ=60)

                            AGTGTCCAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCCCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCT                                                                   <  SRR4302535.4567715/94‑1 (MQ=60)

                              GGTCGAAGAAGCGTAATATCAAATCGGATGGCGATCCGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCC                                                                 <  SRR4302535.1342013/94‑1 (MQ=60)

                               GTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCG                                                                >  SRR4302535.4029188/1‑94 (MQ=60)

                                 CGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCGCGCAGGCATCATCCGCTCCGGG                                                              >  SRR4302535.3749734/1‑94 (MQ=60)

                                  GAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCG                                                             >  SRR4302535.2794856/1‑94 (MQ=60)

                                  GAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCG                                                             >  SRR4302535.4616415/1‑94 (MQ=60)

                                   AAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGT                                                            >  SRR4302535.360184/1‑94 (MQ=60)

                                   AAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGT                                                            >  SRR4302535.3754553/1‑94 (MQ=60)

                                   AAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGT                                                            >  SRR4302535.706502/1‑94 (MQ=60)

                                   AAGAAGCGTAATATCAAACCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGT                                                            <  SRR4302535.325942/94‑1 (MQ=60)

                                     AAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCTATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTA                                                          <  SRR4302535.3713439/94‑1 (MQ=60)

                                      AAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGCTCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAA                                                         >  SRR4302535.3326227/1‑94 (MQ=60)

                                      AAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAA                                                         >  SRR4302535.1023330/1‑94 (MQ=60)

                                         CGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAG                                                      <  SRR4302535.693417/94‑1 (MQ=60)

                                          GTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGC                                                     >  SRR4302535.1861885/1‑94 (MQ=60)

                                          GTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGC                                                     >  SRR4302535.766445/1‑94 (MQ=60)

                                          GTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATAAAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTCACCGC                                                     >  SRR4302535.193435/1‑94 (MQ=60)

                                          GTAATATCAAATCGGATGGCGATACGACGCCTCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGC                                                     <  SRR4302535.1205673/94‑1 (MQ=60)

                                           TAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACGACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCA                                                    >  SRR4302535.39144/1‑94 (MQ=60)

                                           TAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCA                                                    >  SRR4302535.311647/1‑94 (MQ=60)

                                           TAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAAAGCGTACGCCCGCAGGCATCATACGCTCCGGCGTTTAAAGCA                                                    >  SRR4302535.3254702/1‑94 (MQ=60)

                                            AATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCAC                                                   <  SRR4302535.3155177/94‑1 (MQ=60)

                                            AATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCAC                                                   <  SRR4302535.2980067/94‑1 (MQ=60)

                                             ATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACG                                                  <  SRR4302535.3459543/94‑1 (MQ=60)

                                             ATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACG                                                  >  SRR4302535.2931929/1‑94 (MQ=60)

                                               ATCAAATCGGATGGCGATACGACGTCGCCATCCGGTCTTTTTATACAGAGCGTACGCGCGCAGGAATCATCCGCTCCGGCGGTAAACGCACGCT                                                >  SRR4302535.2130415/1‑94 (MQ=57)

                                               ATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCT                                                >  SRR4302535.2130414/1‑94 (MQ=60)

                                                TCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTT                                               <  SRR4302535.3761842/94‑1 (MQ=60)

                                                   AATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACCGCACGCTTTCC                                            >  SRR4302535.1515086/1‑94 (MQ=60)

                                                   AATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCA                                            >  SRR4302535.352610/1‑94 (MQ=60)

                                                     TCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACT                                          <  SRR4302535.2781994/94‑1 (MQ=60)

                                                     ACGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACT                                          <  SRR4302535.1593101/94‑1 (MQ=60)

                                                      CGGATGTCCATCCGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTG                                         <  SRR4302535.2729922/94‑1 (MQ=60)

                                                        GATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCC                                       <  SRR4302535.3094603/94‑1 (MQ=60)

                                                          TGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGT                                     <  SRR4302535.2975370/94‑1 (MQ=60)

                                                          TGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGT                                     >  SRR4302535.2380536/1‑94 (MQ=60)

                                                           GGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTC                                    >  SRR4302535.2230852/1‑94 (MQ=60)

                                                            GCGATACGACGTCGCCATCCGATCTTTTTATACGACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCA                                   <  SRR4302535.1858381/94‑1 (MQ=60)

                                                             CGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCAT                                  >  SRR4302535.3066934/1‑94 (MQ=60)

                                                             CGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCAT                                  <  SRR4302535.3329399/94‑1 (MQ=60)

                                                             CGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCAT                                  >  SRR4302535.3891011/1‑94 (MQ=60)

                                                              GATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATC                                 <  SRR4302535.2631301/94‑1 (MQ=60)

                                                                  CGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTT                             >  SRR4302535.2940882/1‑94 (MQ=60)

                                                                    ACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTC                           <  SRR4302535.360834/94‑1 (MQ=60)

                                                                     CGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCC                          <  SRR4302535.4136658/94‑1 (MQ=60)

                                                                     CGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCC                          <  SRR4302535.189907/94‑1 (MQ=60)

                                                                      GTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCG                         >  SRR4302535.552775/1‑94 (MQ=60)

                                                                        CGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCA                       <  SRR4302535.207407/94‑1 (MQ=60)

                                                                        CGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCA                       >  SRR4302535.2568285/1‑94 (MQ=60)

                                                                          CCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACA                     <  SRR4302535.1618705/94‑1 (MQ=60)

                                                                          CCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACA                     >  SRR4302535.2123965/1‑94 (MQ=60)

                                                                           CATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACAC                    >  SRR4302535.4138423/1‑94 (MQ=60)

                                                                           CATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACAC                    >  SRR4302535.4590549/1‑94 (MQ=60)

                                                                           CATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACAC                    >  SRR4302535.1746004/1‑94 (MQ=60)

                                                                           CATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACAC                    >  SRR4302535.1614107/1‑94 (MQ=60)

                                                                            ATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACA                   >  SRR4302535.937301/1‑94 (MQ=60)

                                                                            ATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACA                   >  SRR4302535.3752857/1‑94 (MQ=60)

                                                                            ATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACA                   >  SRR4302535.1949261/1‑94 (MQ=60)

                                                                             TCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAA                  >  SRR4302535.1031181/1‑94 (MQ=60)

                                                                              CCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAAC                 >  SRR4302535.3559617/1‑94 (MQ=60)

                                                                               CGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACA                >  SRR4302535.37880/1‑94 (MQ=60)

                                                                               CGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACA                >  SRR4302535.2120401/1‑94 (MQ=60)

                                                                               CGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACA                <  SRR4302535.2995166/94‑1 (MQ=60)

                                                                               CGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACA                >  SRR4302535.4612905/1‑94 (MQ=60)

                                                                               CGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACA                >  SRR4302535.4471261/1‑94 (MQ=60)

                                                                                GATCTTTTTATACGACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAG               >  SRR4302535.2409115/1‑94 (MQ=60)

                                                                                GATCTTTTTATACGACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAG               >  SRR4302535.699451/1‑94 (MQ=60)

                                                                                GATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAG               >  SRR4302535.3806148/1‑94 (MQ=60)

                                                                                  TCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCA             >  SRR4302535.644375/1‑94 (MQ=60)

                                                                                  TCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCA             >  SRR4302535.4501876/1‑94 (MQ=60)

                                                                                   CTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCAT            >  SRR4302535.1933383/1‑94 (MQ=60)

                                                                                   CTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCAT            <  SRR4302535.2970456/94‑1 (MQ=60)

                                                                                   CTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCAT            >  SRR4302535.3723674/1‑94 (MQ=60)

                                                                                    TTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATG           <  SRR4302535.1893706/94‑1 (MQ=60)

                                                                                    TTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATG           <  SRR4302535.183246/94‑1 (MQ=60)

                                                                                    TTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATG           <  SRR4302535.841876/94‑1 (MQ=60)

                                                                                    TTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATG           <  SRR4302535.1187953/94‑1 (MQ=60)

                                                                                    TTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATG           <  SRR4302535.2222289/94‑1 (MQ=60)

                                                                                    TTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATG           <  SRR4302535.4535875/94‑1 (MQ=60)

                                                                                      TTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCA         <  SRR4302535.1427288/94‑1 (MQ=60)

                                                                                       TTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCAGGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCAT        >  SRR4302535.1121764/1‑94 (MQ=60)

                                                                                       TTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCAT        <  SRR4302535.1460159/94‑1 (MQ=60)

                                                                                         ATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTC      <  SRR4302535.2576510/94‑1 (MQ=60)

                                                                                         ATACAACGCGTACGCCCGCACGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTC      >  SRR4302535.788734/1‑94 (MQ=60)

                                                                                          TACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTCC     <  SRR4302535.4177706/94‑1 (MQ=60)

                                                                                          TACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTCC     <  SRR4302535.1250462/94‑1 (MQ=60)

                                                                                           ACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTCCG    <  SRR4302535.2619805/94‑1 (MQ=60)

                                                                                            CAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTCCGA   >  SRR4302535.1972718/1‑94 (MQ=60)

                                                                                             AACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTCCGAC  <  SRR4302535.602914/94‑1 (MQ=60)

                                                                                             AACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTCCGAC  >  SRR4302535.692772/1‑94 (MQ=60)

                                                                                             AACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTCCGAC  >  SRR4302535.2801269/1‑94 (MQ=60)

                                                                                             AACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTCCGAC  >  SRR4302535.3985900/1‑94 (MQ=60)

GCGTAAGGCGCTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACGACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTCCGAC  >  NC_000913/3218469‑3218655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`