Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,219,876 G→A G257D (GGC→GAC)  ebgR → EbgR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,219,8760GA99.7% 1000.2 / ‑5.6 289G257D (GGC→GAC) ebgREbgR
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (155/131);  total (156/133)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.08e-01

Only 100 of 298 total aligned reads displayed.

AAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCA  >  NC_000913/3219786‑3219965
                                                                                          |                                                                                         
aaaTGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGAcc                                                                                         <  2:4461017/93‑1 (MQ=255)
aaaTGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGAcc                                                                                         <  1:2469601/93‑1 (MQ=255)
 aaTGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCt                                                                                        <  2:5751853/93‑1 (MQ=255)
 aaTGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCt                                                                                        <  2:493386/93‑1 (MQ=255)
    gCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGaa                                                                                     <  2:3715817/93‑1 (MQ=255)
     cTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAAc                                                                                    >  2:1324122/1‑93 (MQ=255)
      gggCGCGGGAAGACTATGCTAAGGCACTGTCTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATGCATGAACGAGACCTGAACa                                                                                   <  1:4081659/92‑1 (MQ=255)
        gcgcGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATc                                                                                 <  1:1823450/93‑1 (MQ=255)
         cgcgGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATcc                                                                                >  2:2158472/1‑93 (MQ=255)
           cgGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCca                                                                              <  1:1188340/93‑1 (MQ=255)
            gggAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCcac                                                                             >  2:1570391/1‑93 (MQ=255)
            gggAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCcac                                                                             >  2:2824918/1‑93 (MQ=255)
            gggAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCcac                                                                             <  2:2319087/93‑1 (MQ=255)
             ggAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCcaca                                                                            >  2:4537858/1‑93 (MQ=255)
             ggAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCcaca                                                                            >  2:2550232/1‑93 (MQ=255)
              gAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAg                                                                           >  1:4995199/1‑93 (MQ=255)
                aGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGa                                                                         >  2:4335871/1‑93 (MQ=255)
                 gACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGat                                                                        <  1:2326884/93‑1 (MQ=255)
                 gACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGat                                                                        >  2:74002/1‑93 (MQ=255)
                  aCTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCCATTCATGAACGAGACCTGAACATCCCACAGGata                                                                       >  1:6847569/1‑93 (MQ=255)
                  aCTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGata                                                                       <  2:1014639/93‑1 (MQ=255)
                  aCTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGata                                                                       <  2:6272405/93‑1 (MQ=255)
                    tATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATAtt                                                                     <  1:6582937/93‑1 (MQ=255)
                    tATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATAtt                                                                     >  2:667934/1‑93 (MQ=255)
                      tCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTc                                                                   <  2:4152703/93‑1 (MQ=255)
                      tCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTc                                                                   >  2:5236446/1‑93 (MQ=255)
                      tCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTc                                                                   <  1:2756305/93‑1 (MQ=255)
                         gAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCt                                                                <  1:5393023/93‑1 (MQ=255)
                          aaGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCtt                                                               <  1:4614827/93‑1 (MQ=255)
                          aaGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCtt                                                               <  1:3173816/93‑1 (MQ=255)
                           aGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTa                                                              <  1:4929518/93‑1 (MQ=255)
                            ggCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTAt                                                             >  2:6978599/1‑93 (MQ=255)
                             gCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATc                                                            <  2:7567058/93‑1 (MQ=255)
                             gCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATc                                                            <  1:1428657/93‑1 (MQ=255)
                             gCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATc                                                            <  1:6246572/93‑1 (MQ=255)
                             gCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATc                                                            <  2:4771769/93‑1 (MQ=255)
                             gCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATc                                                            >  2:7540291/1‑93 (MQ=255)
                             gCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATc                                                            <  1:2168068/93‑1 (MQ=255)
                              cACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCa                                                           <  2:1665803/93‑1 (MQ=255)
                              cACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCa                                                           >  1:3071097/1‑93 (MQ=255)
                              cACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCa                                                           <  1:3564178/93‑1 (MQ=255)
                               aCTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAg                                                          <  2:5997462/93‑1 (MQ=255)
                                cTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGc                                                         >  1:1905320/1‑93 (MQ=255)
                                cTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGc                                                         >  2:162065/1‑93 (MQ=255)
                                cTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGc                                                         >  1:4524005/1‑93 (MQ=255)
                                cTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGc                                                         >  2:3930471/1‑93 (MQ=255)
                                 tgtttgttGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCg                                                        >  1:7005024/1‑93 (MQ=255)
                                 tgtttgttGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCg                                                        >  1:3415926/1‑93 (MQ=255)
                                  ttttcttGCTTCCGATTCAATTGTTATCCGCATTCTGCGGGCCATTCAGGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGt                                                       <  2:3304286/92‑1 (MQ=255)
                                  gtttgttGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGt                                                       >  1:2940001/1‑93 (MQ=255)
                                     tgttgCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTaa                                                    <  1:7925816/93‑1 (MQ=255)
                                        tgCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGa                                                 >  1:3336831/1‑93 (MQ=255)
                                        tgCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGa                                                 >  2:639199/1‑93 (MQ=255)
                                         gCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGat                                                >  1:3939947/1‑93 (MQ=255)
                                         gCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGaa                                                >  1:4632084/1‑92 (MQ=255)
                                          cTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGata                                               <  2:6546332/93‑1 (MQ=255)
                                          cTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGata                                               <  1:253594/93‑1 (MQ=255)
                                            tCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATc                                             <  2:7223671/93‑1 (MQ=255)
                                              cGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATccc                                           <  1:6658335/93‑1 (MQ=255)
                                               gATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATcccc                                          >  2:3027782/1‑93 (MQ=255)
                                                aTTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCa                                         >  2:3201466/1‑93 (MQ=255)
                                                aTTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCa                                         <  1:3255348/93‑1 (MQ=255)
                                                aTTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCa                                         >  1:7289949/1‑93 (MQ=255)
                                                 ttCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCAc                                        <  1:7582568/93‑1 (MQ=255)
                                                    cATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACcgc                                     >  1:2266932/1‑93 (MQ=255)
                                                    cATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACcgc                                     <  2:7118892/93‑1 (MQ=255)
                                                      ttGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTCACGATATCCCCACcgcgc                                   >  1:7713142/1‑93 (MQ=255)
                                                      ttGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACcgcgc                                   >  2:6096753/1‑93 (MQ=255)
                                                      ttGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACcgcgc                                   >  1:1361268/1‑93 (MQ=255)
                                                       tGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACcgcgcg                                  >  2:771440/1‑93 (MQ=255)
                                                          tATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGCtt                               >  2:4075261/1‑93 (MQ=255)
                                                          tATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGAtt                               >  1:6471018/1‑93 (MQ=255)
                                                          tATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGAtt                               >  1:3631934/1‑93 (MQ=255)
                                                            tCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTa                             <  2:6945143/93‑1 (MQ=255)
                                                            tCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTa                             >  2:6343342/1‑93 (MQ=255)
                                                             cGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTAc                            <  2:1905419/93‑1 (MQ=255)
                                                              ggCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTAcc                           <  1:4016312/93‑1 (MQ=255)
                                                              ggCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTAcc                           >  2:5144510/1‑93 (MQ=255)
                                                              ggCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTAcc                           <  2:975526/93‑1 (MQ=255)
                                                               gCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCt                          >  2:1443192/1‑93 (MQ=255)
                                                                 gTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCttt                        <  1:605678/93‑1 (MQ=255)
                                                                  tACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTc                       >  2:6151324/1‑93 (MQ=255)
                                                                  tACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTc                       >  2:2421148/1‑93 (MQ=255)
                                                                  tACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTc                       >  2:58363/1‑93 (MQ=255)
                                                                  tACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTc                       <  2:5919411/93‑1 (MQ=255)
                                                                   aCTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTcc                      <  1:5677840/93‑1 (MQ=255)
                                                                    cTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTccg                     >  2:5497572/1‑93 (MQ=255)
                                                                    cTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTccg                     >  1:2705881/1‑93 (MQ=255)
                                                                     tGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTccgc                    >  1:4820428/1‑93 (MQ=255)
                                                                        gggCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCcgccgc                 >  1:4398070/1‑93 (MQ=255)
                                                                        gggCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCcgccgc                 >  2:3769084/1‑93 (MQ=255)
                                                                         ggCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGct                >  2:2490638/1‑93 (MQ=255)
                                                                          gCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGctc               >  2:7741102/1‑93 (MQ=255)
                                                                            aaTTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCtctc             >  2:1801252/1‑93 (MQ=255)
                                                                            aaTTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCtctc             <  2:202709/93‑1 (MQ=255)
                                                                              ttCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCa           >  2:3553101/1‑93 (MQ=255)
                                                                                  tGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGt       >  2:1379040/1‑93 (MQ=255)
                                                                                  tGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGt       <  1:7131236/93‑1 (MQ=255)
                                                                                    aaCGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTgc     >  1:510207/1‑93 (MQ=255)
                                                                                    aaCGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTgc     >  1:1156844/1‑93 (MQ=255)
                                                                                          |                                                                                         
AAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCA  >  NC_000913/3219786‑3219965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCA  >  NC_000913/3219783‑3219969
                                                                                             |                                                                                             
AACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGC                                                                                               >  SRR4302543.5640123/1‑94 (MQ=60)
 ACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGAC                                                                                              >  SRR4302543.5096944/1‑94 (MQ=60)
 ACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGAC                                                                                              >  SRR4302543.3794070/1‑94 (MQ=60)
  CAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACC                                                                                             <  SRR4302543.668169/94‑1 (MQ=60)
  CAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACC                                                                                             <  SRR4302543.3417694/94‑1 (MQ=60)
   AAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCT                                                                                            <  SRR4302543.2491894/94‑1 (MQ=60)
   AAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCT                                                                                            <  SRR4302543.2491895/94‑1 (MQ=60)
    AATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTG                                                                                           <  SRR4302543.7077194/94‑1 (MQ=60)
       GCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAAC                                                                                        <  SRR4302543.3104894/94‑1 (MQ=60)
        CTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACA                                                                                       >  SRR4302543.3307696/1‑94 (MQ=60)
         GGGCGCGGGAAGACTATGCTAAGGCACTGTCTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATGCATGAACGAGACCTGAACAT                                                                                      <  SRR4302543.4118249/94‑1 (MQ=60)
           GCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCC                                                                                    <  SRR4302543.1839821/94‑1 (MQ=60)
           GCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCC                                                                                    <  SRR4302543.190616/94‑1 (MQ=60)
             GCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCA                                                                                  >  SRR4302543.2492241/1‑94 (MQ=60)
              CGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCAC                                                                                 <  SRR4302543.1199057/94‑1 (MQ=60)
               GGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACA                                                                                >  SRR4302543.700502/1‑94 (MQ=60)
               GGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACA                                                                                >  SRR4302543.4032601/1‑94 (MQ=60)
                GGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAG                                                                               >  SRR4302543.3847835/1‑94 (MQ=60)
                GGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAG                                                                               >  SRR4302543.5040669/1‑94 (MQ=60)
                GGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAG                                                                               <  SRR4302543.7599049/94‑1 (MQ=60)
                  AAGACTATCCGAAGGCACTGTTTGTTGCTTCCGCTTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGA                                                                             >  SRR4302543.4023507/1‑94 (MQ=60)
                   AGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGAT                                                                            >  SRR4302543.5222382/1‑94 (MQ=60)
                   AGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGAT                                                                            >  SRR4302543.3415142/1‑94 (MQ=60)
                    GACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCCATTCATGAACGAGACCTGAACATCCCACAGGATA                                                                           >  SRR4302543.6912176/1‑94 (MQ=60)
                    GACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATA                                                                           <  SRR4302543.2348021/94‑1 (MQ=60)
                     ACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATAT                                                                          <  SRR4302543.5099294/94‑1 (MQ=60)
                      CTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATT                                                                         <  SRR4302543.2403851/94‑1 (MQ=60)
                       TATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTT                                                                        <  SRR4302543.6644935/94‑1 (MQ=60)
                         TCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCG                                                                      <  SRR4302543.4944464/94‑1 (MQ=60)
                         TCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCG                                                                      <  SRR4302543.2781378/94‑1 (MQ=60)
                          CCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGC                                                                     >  SRR4302543.6212455/1‑94 (MQ=60)
                           CGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCT                                                                    >  SRR4302543.6370423/1‑94 (MQ=60)
                           CGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCT                                                                    <  SRR4302543.2591938/94‑1 (MQ=60)
                            GAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTT                                                                   <  SRR4302543.5442057/94‑1 (MQ=60)
                             AAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTA                                                                  >  SRR4302543.5036308/1‑94 (MQ=60)
                             AAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTA                                                                  <  SRR4302543.4656743/94‑1 (MQ=60)
                             AAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTA                                                                  <  SRR4302543.3202410/94‑1 (MQ=60)
                             AAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTA                                                                  <  SRR4302543.3202409/94‑1 (MQ=60)
                              AGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTAT                                                                 >  SRR4302543.6654331/1‑94 (MQ=60)
                              AGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTAT                                                                 <  SRR4302543.4974426/94‑1 (MQ=60)
                              AGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTAT                                                                 >  SRR4302543.6654332/1‑94 (MQ=60)
                              AGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTAT                                                                 <  SRR4302543.6746378/94‑1 (MQ=60)
                              AGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTAT                                                                 <  SRR4302543.4166965/94‑1 (MQ=60)
                              AGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTAT                                                                 <  SRR4302543.1200331/94‑1 (MQ=60)
                              AGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTCAACATCCCACAGGATATTTCGCTTAT                                                                 <  SRR4302543.1188449/94‑1 (MQ=60)
                                GCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCA                                                               >  SRR4302543.3098712/1‑94 (MQ=60)
                                GCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCA                                                               <  SRR4302543.6305501/94‑1 (MQ=60)
                                GCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCA                                                               <  SRR4302543.1441582/94‑1 (MQ=60)
                                GCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCA                                                               <  SRR4302543.2187494/94‑1 (MQ=60)
                                GCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCA                                                               >  SRR4302543.4662253/1‑94 (MQ=60)
                                 CACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAG                                                              <  SRR4302543.7169906/94‑1 (MQ=60)
                                 CACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAG                                                              <  SRR4302543.3595722/94‑1 (MQ=60)
                                  ACTTTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGC                                                             <  SRR4302543.753430/94‑1 (MQ=60)
                                  ACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGC                                                             >  SRR4302543.8002385/1‑94 (MQ=60)
                                  ACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGC                                                             >  SRR4302543.4565160/1‑94 (MQ=60)
                                  ACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGC                                                             >  SRR4302543.1922474/1‑94 (MQ=60)
                                   CTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCG                                                            >  SRR4302543.4064255/1‑94 (MQ=60)
                                   CTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCG                                                            >  SRR4302543.7071583/1‑94 (MQ=60)
                                   CTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCG                                                            >  SRR4302543.3446503/1‑94 (MQ=60)
                                   CTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCG                                                            >  SRR4302543.5499054/1‑94 (MQ=60)
                                   CTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCG                                                            >  SRR4302543.2822974/1‑94 (MQ=60)
                                    TGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGT                                                           >  SRR4302543.6365493/1‑94 (MQ=60)
                                    TGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGT                                                           >  SRR4302543.2966699/1‑94 (MQ=60)
                                     GTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTT                                                          >  SRR4302543.4846761/1‑94 (MQ=60)
                                        TGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAAC                                                       <  SRR4302543.8000244/94‑1 (MQ=60)
                                          TTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGA                                                     >  SRR4302543.3366895/1‑94 (MQ=60)
                                           TGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGAT                                                    >  SRR4302543.4674185/1‑94 (MQ=60)
                                           TGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGAT                                                    >  SRR4302543.3974783/1‑94 (MQ=60)
                                           TGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGAA                                                    >  SRR4302543.4674186/1‑94 (MQ=60)
                                            GCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATA                                                   <  SRR4302543.4967404/94‑1 (MQ=60)
                                             CTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATAT                                                  <  SRR4302543.91661/94‑1 (MQ=60)
                                             CTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATAT                                                  <  SRR4302543.255806/94‑1 (MQ=60)
                                              TTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATC                                                 >  SRR4302543.552966/1‑94 (MQ=60)
                                               TCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCC                                                <  SRR4302543.2013348/94‑1 (MQ=60)
                                               TCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCC                                                <  SRR4302543.3611846/94‑1 (MQ=60)
                                                 CGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCC                                              <  SRR4302543.6721201/94‑1 (MQ=60)
                                                  GATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCA                                             <  SRR4302543.2358065/94‑1 (MQ=60)
                                                  GATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCA                                             >  SRR4302543.7360314/1‑94 (MQ=60)
                                                   ATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCAC                                            <  SRR4302543.3284780/94‑1 (MQ=60)
                                                   ATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCAC                                            >  SRR4302543.2037330/1‑94 (MQ=60)
                                                   ATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCAC                                            >  SRR4302543.4579315/1‑94 (MQ=60)
                                                   ATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAAACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCAC                                            >  SRR4302543.3284259/1‑94 (MQ=60)
                                                    TTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACC                                           <  SRR4302543.7654647/94‑1 (MQ=60)
                                                    TTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACC                                           <  SRR4302543.7973159/94‑1 (MQ=60)
                                                     TCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCG                                          >  SRR4302543.6818212/1‑94 (MQ=60)
                                                      CCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGC                                         >  SRR4302543.2287629/1‑94 (MQ=60)
                                                      CCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGC                                         >  SRR4302543.3007478/1‑94 (MQ=60)
                                                      CCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGC                                         >  SRR4302543.2302238/1‑94 (MQ=60)
                                                      CCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGC                                         >  SRR4302543.4489486/1‑94 (MQ=60)
                                                       CATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCG                                        >  SRR4302543.2222236/1‑94 (MQ=60)
                                                        ATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCANCNTTAACGATATNCCCANCGCGC                                       >  SRR4302543.7315009/1‑94 (MQ=60)
                                                        ATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTCACGATATCCCCACCGCGC                                       >  SRR4302543.7786097/1‑94 (MQ=60)
                                                        ATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGC                                       >  SRR4302543.1436236/1‑94 (MQ=60)
                                                        ATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGC                                       >  SRR4302543.1373467/1‑94 (MQ=60)
                                                        ATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGC                                       >  SRR4302543.5806895/1‑94 (MQ=60)
                                                        ATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGC                                       >  SRR4302543.3934168/1‑94 (MQ=60)
                                                        ATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGC                                       >  SRR4302543.2808437/1‑94 (MQ=60)
                                                        ATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGC                                       >  SRR4302543.7775600/1‑94 (MQ=60)
                                                        ATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGC                                       >  SRR4302543.7786096/1‑94 (MQ=60)
                                                        ATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGC                                       >  SRR4302543.171917/1‑94 (MQ=60)
                                                        ATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGC                                       >  SRR4302543.7038236/1‑94 (MQ=60)
                                                            CTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATT                                   >  SRR4302543.3663910/1‑94 (MQ=60)
                                                            CTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATT                                   >  SRR4302543.6531983/1‑94 (MQ=60)
                                                              ATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTA                                 >  SRR4302543.4849315/1‑94 (MQ=60)
                                                              ATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTA                                 >  SRR4302543.1415661/1‑94 (MQ=60)
                                                              ATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTA                                 >  SRR4302543.4469281/1‑94 (MQ=60)
                                                              ATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTA                                 >  SRR4302543.7210780/1‑94 (MQ=60)
                                                               GCGGCGTCCTGCGGGCAATTCCTGAACGAGACCTGAACCTCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTAC                                <  SRR4302543.2302676/94‑1 (MQ=60)
                                                                CGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACC                               >  SRR4302543.184418/1‑94 (MQ=60)
                                                                 GGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCT                              <  SRR4302543.4052183/94‑1 (MQ=60)
                                                                 GGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCT                              <  SRR4302543.4052182/94‑1 (MQ=60)
                                                                  GCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTT                             <  SRR4302543.2755628/94‑1 (MQ=60)
                                                                   CGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTT                            <  SRR4302543.2239387/94‑1 (MQ=60)
                                                                    GTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTC                           <  SRR4302543.611217/94‑1 (MQ=60)
                                                                    GTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTC                           >  SRR4302543.3649230/1‑94 (MQ=60)
                                                                     TACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCC                          >  SRR4302543.2774864/1‑94 (MQ=60)
                                                                     TACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCC                          <  SRR4302543.3446767/94‑1 (MQ=60)
                                                                     TACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCC                          <  SRR4302543.3281353/94‑1 (MQ=60)
                                                                     GACTGCTGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCC                          <  SRR4302543.1319439/94‑1 (MQ=60)
                                                                      ACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCG                         >  SRR4302543.5935335/1‑94 (MQ=60)
                                                                      ACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCG                         <  SRR4302543.5729668/94‑1 (MQ=60)
                                                                      ACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCG                         >  SRR4302543.2730434/1‑94 (MQ=60)
                                                                       CTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGC                        >  SRR4302543.4864434/1‑94 (MQ=60)
                                                                          CGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGC                     >  SRR4302543.3540690/1‑94 (MQ=60)
                                                                          CGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGC                     >  SRR4302543.1707053/1‑94 (MQ=60)
                                                                          CGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGC                     >  SRR4302543.4437940/1‑94 (MQ=60)
                                                                           GGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCT                    <  SRR4302543.5027198/94‑1 (MQ=60)
                                                                           GGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCT                    <  SRR4302543.6216794/94‑1 (MQ=60)
                                                                           GGACAATTCATGAACGAGAACTGAACGTCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGACGCT                    <  SRR4302543.1309802/94‑1 (MQ=60)
                                                                            GACAATTCATGAACGAGTCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCTATTTACCTTTCCGCCGCTC                   <  SRR4302543.3358768/94‑1 (MQ=60)
                                                                              CAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTC                 >  SRR4302543.657194/1‑94 (MQ=60)
                                                                               AATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCC                >  SRR4302543.7934045/1‑94 (MQ=60)
                                                                                 TTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCAC              >  SRR4302543.4124602/1‑94 (MQ=60)
                                                                                  TCATGAACGAGACCTGAACATCCCACAGGATATTTCGATTATCAGCGTTAACGATATCCCCACCGCGCGATTTGCCTTTCCGCCGCTCTCCACC             <  SRR4302543.5149750/94‑1 (MQ=60)
                                                                                   CATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCG            >  SRR4302543.4575865/1‑94 (MQ=60)
                                                                                    ATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGT           <  SRR4302543.6570494/94‑1 (MQ=60)
                                                                                    ATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCATTCCGCCGCTCTCCACCGT           >  SRR4302543.2968217/1‑94 (MQ=60)
                                                                                     TGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTG          <  SRR4302543.7200537/94‑1 (MQ=60)
                                                                                     TGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTG          >  SRR4302543.2672057/1‑94 (MQ=60)
                                                                                      GAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGC         >  SRR4302543.514953/1‑94 (MQ=60)
                                                                                      GAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGC         >  SRR4302543.514952/1‑94 (MQ=60)
                                                                                      GAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGC         >  SRR4302543.1167375/1‑94 (MQ=60)
                                                                                       AACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCG        <  SRR4302543.340629/94‑1 (MQ=60)
                                                                                         CGAGACCTGAACATCCCACAGGATATTTCCCTTATCAGCCTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCA      <  SRR4302543.5290861/94‑1 (MQ=60)
                                                                                             ACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCA  >  SRR4302543.4868153/1‑94 (MQ=60)
                                                                                             |                                                                                             
AACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCA  >  NC_000913/3219783‑3219969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑