Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,219,876 G→A G257D (GGC→GAC)  ebgR → EbgR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,219,8760GA99.5% 663.8 / NA 189G257D (GGC→GAC) ebgREbgR
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (103/85);  total (103/86)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 198 total aligned reads displayed.

AAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCA  >  NC_000913/3219786‑3219965
                                                                                          |                                                                                         
aaaTGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGAcc                                                                                         >  2:3390372/1‑93 (MQ=255)
 aaTGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCt                                                                                        <  2:3358632/93‑1 (MQ=255)
  aTGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTg                                                                                       <  1:2878763/93‑1 (MQ=255)
  aTGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGAACTGCGGGCAATTCATGAACGAGACCTg                                                                                       >  1:3146967/1‑93 (MQ=255)
   tGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTATTGCGGGCAATTCATGAACGAGACGTGa                                                                                      >  1:237200/1‑93 (MQ=255)
   tGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGa                                                                                      >  1:3371106/1‑93 (MQ=255)
    gCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGaa                                                                                     <  2:767332/93‑1 (MQ=255)
         cgcgGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATcc                                                                                <  2:2634286/93‑1 (MQ=255)
          gcgGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATccc                                                                               >  1:26486/1‑93 (MQ=255)
            gggAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCcac                                                                             >  1:1750397/1‑93 (MQ=255)
              gAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAg                                                                           >  2:2871543/1‑93 (MQ=255)
                aGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGa                                                                         <  1:3056777/93‑1 (MQ=255)
                 gACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGat                                                                        >  1:4274854/1‑93 (MQ=255)
                 gACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGat                                                                        <  1:1876781/93‑1 (MQ=255)
                 gACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGat                                                                        >  2:4116895/1‑93 (MQ=255)
                 gACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGat                                                                        <  2:418558/93‑1 (MQ=255)
                   cTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGatat                                                                      <  2:2707367/93‑1 (MQ=255)
                    tATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATAtt                                                                     >  2:185414/1‑93 (MQ=255)
                    tATCCGAAGGCACTGTTTGTTGCTTCCGATGCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATAtt                                                                     <  2:1060646/93‑1 (MQ=255)
                     aTCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATAttt                                                                    >  2:2998402/1‑93 (MQ=255)
                      tCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTc                                                                   >  1:2255994/1‑93 (MQ=255)
                      tCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTc                                                                   <  2:2371925/93‑1 (MQ=255)
                        cGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGc                                                                 >  1:1015295/1‑93 (MQ=255)
                         gAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTAGCt                                                                >  1:1598450/1‑93 (MQ=255)
                          aaGGCACTGTTTGTTGCTTCCGCTTCCATTGCTATCGGCGTACTGCGGGCAATTCATGCACGAGACCTGAACATCCCACAGGATATTTCGCtt                                                               >  2:2515025/1‑93 (MQ=255)
                          aaGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCtt                                                               <  1:150390/93‑1 (MQ=255)
                          aaGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCtt                                                               <  2:1242049/93‑1 (MQ=255)
                            ggCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGTTTAt                                                             <  2:1795750/93‑1 (MQ=255)
                            ggCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTAt                                                             <  1:2925353/93‑1 (MQ=255)
                             gCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATc                                                            <  1:72250/93‑1 (MQ=255)
                              cACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCa                                                           <  1:4469771/93‑1 (MQ=255)
                               ccTGTTTGTTGCGTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAg                                                          <  1:4404977/92‑1 (MQ=255)
                               aCTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAg                                                          <  2:3214046/93‑1 (MQ=255)
                               aCTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAg                                                          <  1:22925/93‑1 (MQ=255)
                               aCTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAg                                                          <  1:1001835/93‑1 (MQ=255)
                                cTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGc                                                         >  1:677280/1‑93 (MQ=255)
                                 tgtttgttGCTTCCGATTCTATTGCTATCGGCGTACTGCGGGCAATTCATGACCGAGACCTGAACATCCCACAGGTTATTTCGCTTATCAGCg                                                        >  2:4574664/1‑93 (MQ=255)
                                 tgtttgttGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCg                                                        >  2:88415/1‑93 (MQ=255)
                                 tgtttgttGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCg                                                        >  2:594590/1‑93 (MQ=255)
                                 tgtttgttGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCg                                                        >  2:3490927/1‑93 (MQ=255)
                                  gtttgttGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGt                                                       >  2:3089297/1‑93 (MQ=255)
                                   tttgttGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACGTCCCACAGGATATTTCGCTTATCAGCGtt                                                      >  2:2222081/1‑93 (MQ=255)
                                     tgttgCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTaa                                                    <  2:4677614/93‑1 (MQ=255)
                                     tgttgCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTaa                                                    <  1:1342862/93‑1 (MQ=255)
                                      gttgCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAAc                                                   >  2:1186527/1‑93 (MQ=255)
                                      gttgCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAAc                                                   >  1:3314577/1‑93 (MQ=255)
                                      gttgCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAAc                                                   >  1:566756/1‑93 (MQ=255)
                                      gttgCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAAc                                                   >  1:499005/1‑93 (MQ=255)
                                      gttgCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAAc                                                   >  2:4507779/1‑93 (MQ=255)
                                       ttgCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACg                                                  >  1:3844564/1‑93 (MQ=255)
                                        tgCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGa                                                 <  1:4232537/93‑1 (MQ=255)
                                         gCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGat                                                <  2:148886/93‑1 (MQ=255)
                                          cTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGata                                               <  1:3507070/93‑1 (MQ=255)
                                          cTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGata                                               >  1:899847/1‑93 (MQ=255)
                                           ttCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGatat                                              >  2:3887275/1‑93 (MQ=255)
                                           ttCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGatat                                              <  1:3261489/93‑1 (MQ=255)
                                             ccGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATcc                                            <  1:1982081/93‑1 (MQ=255)
                                              cGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATccc                                           <  2:226464/93‑1 (MQ=255)
                                                aTTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCa                                         >  1:482548/1‑93 (MQ=255)
                                                aTTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCa                                         <  1:3532962/93‑1 (MQ=255)
                                                 ttCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCAc                                        >  2:2886855/1‑93 (MQ=255)
                                                 ttCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCAc                                        >  1:2446778/1‑93 (MQ=255)
                                                  tCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCAcc                                       >  1:3570581/1‑93 (MQ=255)
                                                    cATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACcgc                                     >  1:2581224/1‑93 (MQ=255)
                                                     aTTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACcgcg                                    >  2:103116/1‑93 (MQ=255)
                                                     aTTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACcgcg                                    >  1:246744/1‑93 (MQ=255)
                                                       tGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACcgcgcg                                  <  2:4360668/93‑1 (MQ=255)
                                                        gCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGa                                 <  2:3524973/93‑1 (MQ=255)
                                                         cTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGAt                                >  1:4684980/1‑93 (MQ=255)
                                                          tATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGAtt                               <  2:3688923/93‑1 (MQ=255)
                                                          tATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGAtt                               >  1:1819049/1‑93 (MQ=255)
                                                          tATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGAtt                               >  1:2800212/1‑93 (MQ=255)
                                                          tATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGAtt                               >  1:3701767/1‑93 (MQ=255)
                                                          tATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGAtt                               >  2:564433/1‑93 (MQ=255)
                                                          tATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGAtt                               >  2:131169/1‑93 (MQ=255)
                                                          tATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGAtt                               >  1:4056226/1‑93 (MQ=255)
                                                           aTCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGAttt                              >  2:2789250/1‑93 (MQ=255)
                                                            tCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACTGCGCGATTTa                             >  2:2862122/1‑93 (MQ=255)
                                                              ggCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTAcc                           >  1:3189263/1‑93 (MQ=255)
                                                              ggCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTAcc                           <  1:621671/93‑1 (MQ=255)
                                                              ggCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTAcc                           <  2:1180617/93‑1 (MQ=255)
                                                              ggCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTAcc                           >  1:3087442/1‑93 (MQ=255)
                                                                 gTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCttt                        <  2:1444524/93‑1 (MQ=255)
                                                                  tACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGCTTTACCTTTc                       >  2:1910620/1‑93 (MQ=255)
                                                                  tACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTc                       <  1:3941966/93‑1 (MQ=255)
                                                                  tACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTc                       <  1:1203155/93‑1 (MQ=255)
                                                                    cTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTccg                     >  2:4026280/1‑93 (MQ=255)
                                                                      gCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTAACAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTccgcc                   >  1:1671417/1‑93 (MQ=255)
                                                                        gggCATTTCATGACCGCGACCTGACCATCCCACAGGATATTACGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCcgccgc                 <  2:4687966/93‑1 (MQ=255)
                                                                         ggCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGct                <  1:1700279/93‑1 (MQ=255)
                                                                            aaTTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCtctc             >  1:997365/1‑93 (MQ=255)
                                                                            aaTTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCtctc             >  1:2600481/1‑93 (MQ=255)
                                                                             aTTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTcc            >  1:2152000/1‑93 (MQ=255)
                                                                              ttCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCa           >  1:4114903/1‑93 (MQ=255)
                                                                                cATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCAcc         >  1:4605167/1‑93 (MQ=255)
                                                                                 aTGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCg        >  1:1646467/1‑93 (MQ=255)
                                                                                  tGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGt       >  1:807440/1‑93 (MQ=255)
                                                                                  tGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGt       >  2:3610462/1‑93 (MQ=255)
                                                                                    aaCGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTgc     <  2:1915666/93‑1 (MQ=255)
                                                                                       gagaCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCa  >  2:3806736/1‑93 (MQ=255)
                                                                                          |                                                                                         
AAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCA  >  NC_000913/3219786‑3219965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATC  >  NC_000913/3219784‑3219967
                                                                                            |                                                                                           
ACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGGACTGCGGGCAATTCATGAACGAGAC                                                                                            >  SRR4302535.3050724/1‑94 (MQ=60)
 CAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACC                                                                                           <  SRR4302535.1520146/94‑1 (MQ=60)
   AATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGAACTGCGGGCAATTCATGAACGAGACCTG                                                                                         >  SRR4302535.3168848/1‑94 (MQ=60)
    ATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTATTGCGGGCAATTCATGAACGAGACGTGA                                                                                        >  SRR4302535.238835/1‑94 (MQ=60)
    ATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGA                                                                                        >  SRR4302535.3394558/1‑94 (MQ=60)
    ATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGA                                                                                        >  SRR4302535.67167/1‑94 (MQ=60)
    ATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGA                                                                                        <  SRR4302535.2899147/94‑1 (MQ=60)
     TGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAA                                                                                       <  SRR4302535.1251639/94‑1 (MQ=60)
           CGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCC                                                                                 >  SRR4302535.284541/1‑94 (MQ=60)
           CGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCC                                                                                 >  SRR4302535.26693/1‑94 (MQ=60)
           CGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCC                                                                                 <  SRR4302535.2623880/94‑1 (MQ=60)
            GCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCA                                                                                <  SRR4302535.4488811/94‑1 (MQ=60)
             CGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCAC                                                                               >  SRR4302535.1763055/1‑94 (MQ=60)
             CGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCAC                                                                               <  SRR4302535.4390766/94‑1 (MQ=60)
              GGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACA                                                                              >  SRR4302535.859799/1‑94 (MQ=60)
              GGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACA                                                                              <  SRR4302535.3592316/94‑1 (MQ=60)
               GGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAG                                                                             <  SRR4302535.3057647/94‑1 (MQ=60)
                  AGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGAT                                                                          >  SRR4302535.4304114/1‑94 (MQ=60)
                  AGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGAT                                                                          <  SRR4302535.3078169/94‑1 (MQ=60)
                  AGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGAT                                                                          >  SRR4302535.1828546/1‑94 (MQ=60)
                   GACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATA                                                                         <  SRR4302535.1890295/94‑1 (MQ=60)
                   GACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATA                                                                         <  SRR4302535.201933/94‑1 (MQ=60)
                    ACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATAT                                                                        <  SRR4302535.1102771/94‑1 (MQ=60)
                      TATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTT                                                                      <  SRR4302535.168444/94‑1 (MQ=60)
                      TATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTT                                                                      <  SRR4302535.763359/94‑1 (MQ=60)
                       ATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTC                                                                     >  SRR4302535.2272057/1‑94 (MQ=60)
                         CCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGC                                                                   >  SRR4302535.1022514/1‑94 (MQ=60)
                          CGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTAGCT                                                                  >  SRR4302535.1609979/1‑94 (MQ=60)
                           GAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTT                                                                 >  SRR4302535.3872244/1‑94 (MQ=60)
                            AAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTA                                                                <  SRR4302535.151438/94‑1 (MQ=60)
                            AAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTA                                                                >  SRR4302535.2376394/1‑94 (MQ=60)
                             AGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTAT                                                               >  SRR4302535.602144/1‑94 (MQ=60)
                              GGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATC                                                              <  SRR4302535.2945976/94‑1 (MQ=60)
                               GCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCA                                                             <  SRR4302535.1726488/94‑1 (MQ=60)
                               GCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCA                                                             <  SRR4302535.72776/94‑1 (MQ=60)
                               GCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCA                                                             <  SRR4302535.2612008/94‑1 (MQ=60)
                                CACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAG                                                            >  SRR4302535.2299283/1‑94 (MQ=60)
                                CACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAG                                                            <  SRR4302535.4500440/94‑1 (MQ=60)
                                 CCTGTTTGTTGCGTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGC                                                           <  SRR4302535.4435124/94‑1 (MQ=60)
                                 ACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGC                                                           >  SRR4302535.682015/1‑94 (MQ=60)
                                 ACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGC                                                           >  SRR4302535.1572511/1‑94 (MQ=60)
                                 ACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGC                                                           <  SRR4302535.2579933/94‑1 (MQ=60)
                                 ACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGC                                                           <  SRR4302535.23104/94‑1 (MQ=60)
                                 ACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGC                                                           <  SRR4302535.1008953/94‑1 (MQ=60)
                                  CTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCG                                                          >  SRR4302535.3596866/1‑94 (MQ=60)
                                  CTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCG                                                          >  SRR4302535.140650/1‑94 (MQ=60)
                                       TGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAAC                                                     >  SRR4302535.570724/1‑94 (MQ=60)
                                       TGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAAC                                                     >  SRR4302535.3337637/1‑94 (MQ=60)
                                       TGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAAC                                                     >  SRR4302535.3112953/1‑94 (MQ=60)
                                       TGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAAC                                                     <  SRR4302535.1352447/94‑1 (MQ=60)
                                       TGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAAC                                                     >  SRR4302535.502505/1‑94 (MQ=60)
                                        TTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACG                                                    <  SRR4302535.1664114/94‑1 (MQ=60)
                                        GTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACG                                                    >  SRR4302535.3871041/1‑94 (MQ=60)
                                        GTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACG                                                    <  SRR4302535.4277990/94‑1 (MQ=60)
                                          TGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGAT                                                  <  SRR4302535.4261502/94‑1 (MQ=60)
                                           GCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATA                                                 >  SRR4302535.906219/1‑94 (MQ=60)
                                            CTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATAT                                                <  SRR4302535.3531440/94‑1 (MQ=60)
                                            CTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATAT                                                >  SRR4302535.2943778/1‑94 (MQ=60)
                                            CTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATAT                                                >  SRR4302535.405986/1‑94 (MQ=60)
                                            CTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATAT                                                <  SRR4302535.4587958/94‑1 (MQ=60)
                                             TTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATC                                               <  SRR4302535.3097945/94‑1 (MQ=60)
                                             TTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATC                                               <  SRR4302535.3284148/94‑1 (MQ=60)
                                               CCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCC                                             <  SRR4302535.1996351/94‑1 (MQ=60)
                                                CGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCC                                            >  SRR4302535.1000734/1‑94 (MQ=60)
                                                CGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCC                                            >  SRR4302535.4133632/1‑94 (MQ=60)
                                                 GATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCA                                           >  SRR4302535.485932/1‑94 (MQ=60)
                                                 GATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCA                                           >  SRR4302535.3763989/1‑94 (MQ=60)
                                                  ATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCAC                                          >  SRR4302535.2464400/1‑94 (MQ=60)
                                                  ATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCAC                                          <  SRR4302535.3557491/94‑1 (MQ=60)
                                                   TTCCATTGCTATCGGCGTACTGCGGGCAATTCATGATCGAGACCTGAACATCCCACAGGATATTTAGCTTATCCGCGTTCACGATATattccac                                         >  SRR4302535.4260452/1‑87 (MQ=60)
                                                   TTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACC                                         >  SRR4302535.3595342/1‑94 (MQ=60)
                                                     CCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGC                                       >  SRR4302535.2208871/1‑94 (MQ=60)
                                                     CCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGC                                       >  SRR4302535.2599765/1‑94 (MQ=60)
                                                      CATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCG                                      >  SRR4302535.248435/1‑94 (MQ=60)
                                                        TTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCG                                    <  SRR4302535.4223146/94‑1 (MQ=60)
                                                        TTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCG                                    <  SRR4302535.3365552/94‑1 (MQ=60)
                                                          GCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGAT                                  >  SRR4302535.4716687/1‑94 (MQ=60)
                                                           CTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCccaccaccttccccttatcaccgctaacgatatcaccaccacgcgatt                                 >  SRR4302535.3283118/1‑46 (MQ=60)
                                                           CTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATT                                 >  SRR4302535.1832194/1‑94 (MQ=60)
                                                           CTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATT                                 >  SRR4302535.4083996/1‑94 (MQ=60)
                                                           CTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATT                                 >  SRR4302535.3727404/1‑94 (MQ=60)
                                                           CTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATT                                 >  SRR4302535.2820133/1‑94 (MQ=60)
                                                           CTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATT                                 <  SRR4302535.1905722/94‑1 (MQ=60)
                                                            TATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTT                                >  SRR4302535.471586/1‑94 (MQ=60)
                                                              TCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTAC                              <  SRR4302535.3536292/94‑1 (MQ=60)
                                                               CGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACC                             >  SRR4302535.3108943/1‑94 (MQ=60)
                                                               CGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACC                             <  SRR4302535.3196918/94‑1 (MQ=60)
                                                               CGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACC                             >  SRR4302535.3211317/1‑94 (MQ=60)
                                                                GGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCT                            <  SRR4302535.625987/94‑1 (MQ=60)
                                                                GGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCT                            <  SRR4302535.477001/94‑1 (MQ=60)
                                                                  CGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTT                          >  SRR4302535.570063/1‑94 (MQ=60)
                                                                   GTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTC                         >  SRR4302535.2477183/1‑94 (MQ=60)
                                                                    TACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCC                        <  SRR4302535.3968873/94‑1 (MQ=60)
                                                                    TACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCC                        <  SRR4302535.1211792/94‑1 (MQ=60)
                                                                     ACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCG                       <  SRR4302535.492154/94‑1 (MQ=60)
                                                                      CTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTCATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGC                      <  SRR4302535.3322307/94‑1 (MQ=60)
                                                                       TGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTAACAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCC                     >  SRR4302535.1683516/1‑94 (MQ=60)
                                                                           GGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTC                 <  SRR4302535.1712563/94‑1 (MQ=60)
                                                                             CAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTC               >  SRR4302535.2619147/1‑94 (MQ=60)
                                                                             CAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTC               >  SRR4302535.1004444/1‑94 (MQ=60)
                                                                              AATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCC              >  SRR4302535.2167469/1‑94 (MQ=60)
                                                                               ATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCA             >  SRR4302535.4143070/1‑94 (MQ=60)
                                                                                TTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCAC            <  SRR4302535.1888957/94‑1 (MQ=60)
                                                                                 TCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACC           >  SRR4302535.4636470/1‑94 (MQ=60)
                                                                                 TCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACC           <  SRR4302535.2794247/94‑1 (MQ=60)
                                                                                 TCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACC           >  SRR4302535.343587/1‑94 (MQ=60)
                                                                                  CATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCG          >  SRR4302535.1658365/1‑94 (MQ=60)
                                                                                   ATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGT         <  SRR4302535.2419997/94‑1 (MQ=60)
                                                                                   ATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGT         >  SRR4302535.813099/1‑94 (MQ=60)
                                                                                         GAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCAT   <  SRR4302535.1626293/94‑1 (MQ=60)
                                                                                          AGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATC  >  SRR4302535.1515686/1‑94 (MQ=60)
                                                                                            |                                                                                           
ACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATC  >  NC_000913/3219784‑3219967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑