Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
1873 |
183.1 |
9376148 |
98.2% |
9207377 |
93.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,222,944 |
4 bp→AGCA |
coding (2671‑2674/3093 nt) |
b3076 → |
evolved beta‑D‑galactosidase alpha subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,222,944 | 0 | C | A | 100.0%
| 561.7
/ NA
| 155 | Q891S (CAA→AGC) | b3076 | evolved beta‑D‑galactosidase alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (69/86); total (69/86) |
| * | NC_000913 | 3,222,945 | 0 | A | G | 99.4%
| 484.8
/ ‑4.5
| 155 | Q891S (CAA→AGC) | b3076 | evolved beta‑D‑galactosidase alpha subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (69/85); minor base T (0/1); total (69/86) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01 |
| * | NC_000913 | 3,222,946 | 0 | A | C | 100.0%
| 490.1
/ NA
| 155 | Q891S (CAA→AGC) | b3076 | evolved beta‑D‑galactosidase alpha subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (69/86); total (69/86) |
| * | NC_000913 | 3,222,947 | 0 | G | A | 99.4%
| 542.5
/ NA
| 155 | A892T (GCC→ACC) | b3076 | evolved beta‑D‑galactosidase alpha subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (68/86); minor base C (1/0); total (69/86) |
| Fisher's exact test for biased strand distribution p-value = 4.45e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 158 total aligned reads displayed.
GATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCCAAGCCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACT > NC_000913/3222863‑3223027
||||
gATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGAt > 1:106484/1‑93 (MQ=255)
aTCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATg > 2:1193883/1‑93 (MQ=255)
tCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCCCCGTCGATGc > 2:2274211/1‑93 (MQ=255)
tCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGc < 2:2598767/93‑1 (MQ=255)
cAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGcc > 2:4031627/1‑93 (MQ=255)
cAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGcc < 2:171021/93‑1 (MQ=255)
cAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGcc < 2:4551444/93‑1 (MQ=255)
aGGTGGCGTATTACGGTCGTGGACCGGGCGACAACTACGCCAACAGCCAGCAGGCTAACATCATCGATCTCTGGCGCAGCACCGTCGATGCCa < 2:4687916/93‑1 (MQ=255)
aGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCa < 2:2818397/93‑1 (MQ=255)
aGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCa < 1:4108472/93‑1 (MQ=255)
ggTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCAt > 1:596564/1‑93 (MQ=255)
ggTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCAt < 2:103929/93‑1 (MQ=255)
ggTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCAt < 1:2675394/93‑1 (MQ=255)
tGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGt > 2:1829077/1‑93 (MQ=255)
tGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGt > 2:193016/1‑93 (MQ=255)
ggCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGtt < 1:1724725/93‑1 (MQ=255)
ggCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGtt < 2:2418937/93‑1 (MQ=255)
ggCGTATTACGGTCGTGGACAGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGtt < 1:1794791/93‑1 (MQ=255)
gCGTATTCCGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTc < 2:28650/93‑1 (MQ=255)
gTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCga < 2:2118105/93‑1 (MQ=255)
tATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCgag > 1:1849287/1‑93 (MQ=255)
tATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCgag > 2:4482718/1‑93 (MQ=255)
aTTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCgaga > 2:451289/1‑93 (MQ=255)
aTTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCgaga > 2:451288/1‑93 (MQ=255)
aTTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCgaga > 2:3736075/1‑93 (MQ=255)
ttACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGaa < 2:4330055/93‑1 (MQ=255)
tACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAAc < 1:2798304/93‑1 (MQ=255)
cGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTa > 2:3231660/1‑93 (MQ=255)
cGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTa < 2:3127317/93‑1 (MQ=255)
ggTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTAt < 1:3713986/93‑1 (MQ=255)
ggTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTAt > 2:2043927/1‑93 (MQ=255)
gTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATc > 2:2357930/1‑93 (MQ=255)
tCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATcc < 1:1173469/93‑1 (MQ=255)
cGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATccc < 2:1107400/93‑1 (MQ=255)
cGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATccc < 1:1855206/93‑1 (MQ=255)
gTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCt < 1:3190637/93‑1 (MQ=255)
ggACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCATCACCGTCGATGCCATGTTCGAGAACTATCCCTTc < 1:1621430/93‑1 (MQ=255)
ggACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTc < 1:588135/93‑1 (MQ=255)
ggACCGGCCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTc < 2:833891/93‑1 (MQ=255)
gACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTcc > 2:2174858/1‑93 (MQ=255)
ggCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGcgg < 2:2445821/93‑3 (MQ=255)
cGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaa < 1:351662/93‑1 (MQ=255)
gAAAACTCCGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaac < 2:3424128/93‑1 (MQ=255)
gAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaac > 1:796956/1‑93 (MQ=255)
gAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaac < 2:1451393/93‑1 (MQ=255)
gAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaac < 1:226170/93‑1 (MQ=255)
aaaaCTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaaca > 1:3790785/1‑93 (MQ=255)
aaaCTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaacaa > 2:2092541/1‑93 (MQ=255)
aaaCTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaacaa > 1:4267467/1‑93 (MQ=255)
aaaCTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaacaa < 1:2746624/93‑1 (MQ=255)
aaaCTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaacaa < 2:4210950/93‑1 (MQ=255)
aaCTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaacaac < 2:3935649/93‑1 (MQ=255)
aaCTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaacaac < 2:4233741/93‑1 (MQ=255)
cTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACgg < 2:64402/93‑1 (MQ=255)
cTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACgg > 1:4105675/1‑93 (MQ=255)
caCGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGt < 2:3335580/92‑1 (MQ=255)
cGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCTAGAACTATCCCTTCCCGCAGAACAACGGTaa > 1:4486138/1‑93 (MQ=255)
cGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTaa < 2:3309945/93‑1 (MQ=255)
gCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAAc < 1:401912/93‑1 (MQ=255)
cccGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAAc < 1:2424751/92‑1 (MQ=255)
ccGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAAcc < 1:18419/93‑1 (MQ=255)
cGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCg > 1:972134/1‑93 (MQ=255)
gACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGt > 1:1469297/1‑93 (MQ=255)
aCAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGTTAACCGTc < 2:4579253/93‑1 (MQ=255)
aCAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTc > 2:3998594/1‑93 (MQ=255)
aCAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTc < 1:50935/93‑1 (MQ=255)
agccagcAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAg > 1:1958733/1‑93 (MQ=255)
ccagcAGTCTAACTTCATCGATAGCTGGCGCAGCCCCGTCGATGCGAGGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCa < 2:2754523/93‑1 (MQ=255)
ccagcAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCa < 1:3471932/93‑1 (MQ=255)
cagcagGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCAt < 1:3934834/93‑1 (MQ=255)
cagcagGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCAt > 1:3116744/1‑93 (MQ=255)
agcagGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATg < 2:4096012/93‑1 (MQ=255)
agcagGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATg < 1:350724/93‑1 (MQ=255)
agcagGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATg > 1:3876648/1‑93 (MQ=255)
agcagGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATg < 2:2180655/93‑1 (MQ=255)
gcagGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGt > 2:1428849/1‑93 (MQ=255)
gcagGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGt > 1:1129658/1‑93 (MQ=255)
cagGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTc < 2:3886422/93‑1 (MQ=255)
gCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGc < 2:490023/93‑1 (MQ=255)
tAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTg > 1:3840325/1‑93 (MQ=255)
aaCATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTgg < 2:3610169/93‑1 (MQ=255)
aaCATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTgg < 1:500073/93‑1 (MQ=255)
aCATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGa > 2:3480225/1‑93 (MQ=255)
aCATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGa > 1:4011762/1‑93 (MQ=255)
atcatcGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACg < 2:673707/93‑1 (MQ=255)
atcatcGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACg > 2:4337827/1‑93 (MQ=255)
catcGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGc < 2:952283/93‑1 (MQ=255)
catcGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGc < 2:3721092/93‑1 (MQ=255)
atcGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCa > 2:1882939/1‑93 (MQ=255)
tcGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCAc < 1:2921648/93‑1 (MQ=255)
tcGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCAc < 1:2343022/93‑1 (MQ=255)
tcGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCAc < 1:2236208/93‑1 (MQ=255)
tcGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCAc < 2:928567/93‑1 (MQ=255)
gtatCTGGCGCATCCCCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGa < 1:4586049/92‑1 (MQ=255)
atatCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGa > 1:3266891/1‑93 (MQ=255)
atatCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGa < 2:520508/93‑1 (MQ=255)
atatCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGa < 1:2062096/93‑1 (MQ=255)
atatCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGa < 1:1096581/93‑1 (MQ=255)
tatCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGAc < 1:2150346/93‑1 (MQ=255)
atCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACt < 2:1556196/93‑1 (MQ=255)
||||
GATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCCAAGCCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACT > NC_000913/3222863‑3223027
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
AACGGCGAATACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGC‑‑CAAGCCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACT > NC_000913/3222851‑3223027
|
AACGGCGAATACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGC‑‑A > SRR4302535.4587281/1‑94 (MQ=60)
AACGGCGAATACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGC‑‑A > SRR4302535.849663/1‑94 (MQ=60)
ATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGC < SRR4302535.396632/94‑1 (MQ=60)
CAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCA < SRR4302535.3528696/94‑1 (MQ=60)
AGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCAT < SRR4302535.4136599/94‑1 (MQ=60)
AGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCAT > SRR4302535.600744/1‑94 (MQ=60)
AGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCAT > SRR4302535.1351395/1‑94 (MQ=60)
GGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATG < SRR4302535.2694536/94‑1 (MQ=60)
GGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTC < SRR4302535.1737199/94‑1 (MQ=60)
GGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTC < SRR4302535.1855614/94‑1 (MQ=60)
GGCGTATTACGGTCGTGGACAGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTC < SRR4302535.1807765/94‑1 (MQ=60)
GTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAG > SRR4302535.1862614/1‑94 (MQ=60)
GTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAG < SRR4302535.3736793/94‑1 (MQ=60)
ATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAA > SRR4302535.3831209/1‑94 (MQ=60)
TACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACT < SRR4302535.2818217/94‑1 (MQ=60)
CGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTAT > SRR4302535.4229347/1‑94 (MQ=60)
GGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATC < SRR4302535.3739717/94‑1 (MQ=60)
TCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCC < SRR4302535.1181846/94‑1 (MQ=60)
CGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCT < SRR4302535.1868572/94‑1 (MQ=60)
CGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCT < SRR4302535.522637/94‑1 (MQ=60)
GTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTT > SRR4302535.1895030/1‑94 (MQ=60)
GTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTT < SRR4302535.3212703/94‑1 (MQ=60)
GGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCATCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCC < SRR4302535.1633131/94‑1 (MQ=60)
GGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCC < SRR4302535.592257/94‑1 (MQ=60)
ACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCG < SRR4302535.977228/94‑1 (MQ=60)
CGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAAC > SRR4302535.802552/1‑94 (MQ=60)
CGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAAC < SRR4302535.354087/94‑1 (MQ=60)
GAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACA < SRR4302535.227723/94‑1 (MQ=60)
GAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACA > SRR4302535.3816924/1‑94 (MQ=60)
AAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAA > SRR4302535.4296687/1‑94 (MQ=60)
AAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAAC < SRR4302535.2766203/94‑1 (MQ=60)
AAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAAC > SRR4302535.2903694/1‑94 (MQ=60)
ACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGG > SRR4302535.4133786/1‑94 (MQ=60)
ACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGG < SRR4302535.3959462/94‑1 (MQ=60)
ACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCTAGAACTATCCCTTCCCGCAGAACAACGGTAA > SRR4302535.4516933/1‑94 (MQ=60)
GCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACC < SRR4302535.404730/94‑1 (MQ=60)
CCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACC < SRR4302535.2442222/94‑1 (MQ=60)
CCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCG < SRR4302535.18557/94‑1 (MQ=60)
CCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCG > SRR4302535.979031/1‑94 (MQ=60)
CGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGT > SRR4302535.1479822/1‑94 (MQ=60)
ACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCA < SRR4302535.51309/94‑1 (MQ=60)
CAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAG > SRR4302535.1972832/1‑94 (MQ=60)
CAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAG > SRR4302535.1803931/1‑94 (MQ=60)
GCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCA > SRR4302535.4073421/1‑94 (MQ=60)
CCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCAT > SRR4302535.3138442/1‑94 (MQ=60)
CCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCAT < SRR4302535.3496056/94‑1 (MQ=60)
CCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCAT > SRR4302535.4510394/1‑94 (MQ=60)
CAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATG > SRR4302535.3903274/1‑94 (MQ=60)
CAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATG < SRR4302535.3961683/94‑1 (MQ=60)
AGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGT < SRR4302535.353141/94‑1 (MQ=60)
AGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGT > SRR4302535.1137722/1‑94 (MQ=60)
GCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTC < SRR4302535.2748535/94‑1 (MQ=60)
CAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCC < SRR4302535.1123128/94‑1 (MQ=60)
AGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCG > SRR4302535.2633040/1‑94 (MQ=60)
GGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGC > SRR4302535.2281369/1‑94 (MQ=60)
CTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTG > SRR4302535.3866789/1‑94 (MQ=60)
CTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTG < SRR4302535.1685348/94‑1 (MQ=60)
TAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGG > SRR4302535.474387/1‑94 (MQ=60)
AACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGA < SRR4302535.503577/94‑1 (MQ=60)
AACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGA < SRR4302535.2117461/94‑1 (MQ=60)
AACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGA > SRR4302535.4039207/1‑94 (MQ=60)
ATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGG > SRR4302535.3203935/1‑94 (MQ=60)
TCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACT < SRR4302535.2359789/94‑1 (MQ=60)
TCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACT < SRR4302535.2942258/94‑1 (MQ=60)
TCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACT < SRR4302535.2252116/94‑1 (MQ=60)
CGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTG > SRR4302535.335082/1‑94 (MQ=60)
GATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGA > SRR4302535.3289587/1‑94 (MQ=60)
GATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGA > SRR4302535.743692/1‑94 (MQ=60)
ATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGAC > SRR4302535.779809/1‑94 (MQ=60)
ATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGAC < SRR4302535.2076956/94‑1 (MQ=60)
ATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGAC > SRR4302535.141185/1‑94 (MQ=60)
ATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGAC < SRR4302535.1104387/94‑1 (MQ=60)
TATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACT < SRR4302535.2165941/94‑1 (MQ=60)
TATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACT < SRR4302535.2165810/94‑1 (MQ=60)
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AACGGCGAATACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGC‑‑CAAGCCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACT > NC_000913/3222851‑3223027
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| Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
