Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,222,944 4 bp→AGCA coding (2671‑2674/3093 nt) b3076 → evolved beta‑D‑galactosidase alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,222,9440CA99.6% 936.7 / NA 259Q891S (CAA→AGC) b3076evolved beta‑D‑galactosidase alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (121/137);  total (121/138)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009133,222,9450AG100.0% 808.7 / NA 259Q891S (CAA→AGC) b3076evolved beta‑D‑galactosidase alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (121/138);  total (121/138)
*NC_0009133,222,9460AC100.0% 810.9 / NA 259Q891S (CAA→AGC) b3076evolved beta‑D‑galactosidase alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (121/138);  total (121/138)
*NC_0009133,222,9470GA100.0% 896.4 / NA 259A892T (GCC→ACC) b3076evolved beta‑D‑galactosidase alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (121/138);  total (121/138)

Only 100 of 268 total aligned reads displayed.

GGCGAATACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCCAAGCCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACT  >  NC_000913/3222854‑3223027
                                                                                          ||||                                                                                
ggCGAATACAATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGccgc                                                                                   >  1:5310216/1‑91 (MQ=255)
          aTCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATg                                                                         >  1:6251623/1‑93 (MQ=255)
          aTCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATg                                                                         >  2:7202288/1‑93 (MQ=255)
          aTCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATg                                                                         >  1:4620138/1‑93 (MQ=255)
          aTCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATg                                                                         >  1:1194218/1‑93 (MQ=255)
          aTCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCAAGCCGGCACAAATCATCGATATCTGGCGCAGCACCGTCGAtc                                                                         >  1:2192201/1‑92 (MQ=255)
           tCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGc                                                                        >  1:3551427/1‑93 (MQ=255)
           tCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGc                                                                        >  2:4602946/1‑93 (MQ=255)
            cAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGcc                                                                       <  1:3681/93‑1 (MQ=255)
            cAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGcc                                                                       <  2:2106911/93‑1 (MQ=255)
             aGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCa                                                                      >  2:3602182/1‑93 (MQ=255)
              ggTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCAt                                                                     >  2:7328606/1‑93 (MQ=255)
              ggTGGCGTATTACGGTCGTGGACCGAGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCAt                                                                     <  2:3237012/93‑1 (MQ=255)
               gTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATg                                                                    <  1:1543060/93‑1 (MQ=255)
                tGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGt                                                                   >  2:3114098/1‑93 (MQ=255)
                tGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGt                                                                   <  2:1645685/93‑1 (MQ=255)
                tGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGt                                                                   >  1:5582792/1‑93 (MQ=255)
                  gCGTATTGCGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTc                                                                 <  1:4587193/93‑1 (MQ=255)
                  gCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTc                                                                 <  1:14251/93‑1 (MQ=255)
                    gTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCga                                                               >  1:6348335/1‑93 (MQ=255)
                     tATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCgag                                                              >  2:5601404/1‑93 (MQ=255)
                     tATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCgag                                                              >  1:208337/1‑93 (MQ=255)
                       ttACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGaa                                                            >  2:7133416/1‑93 (MQ=255)
                       ttACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGaa                                                            >  2:7455894/1‑93 (MQ=255)
                       ttACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGaa                                                            >  1:5937361/1‑93 (MQ=255)
                       ttACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGaa                                                            <  2:3436424/93‑1 (MQ=255)
                       ttACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGaa                                                            <  1:5683721/93‑1 (MQ=255)
                        tACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAAc                                                           <  1:7483406/93‑1 (MQ=255)
                        tACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAAc                                                           >  1:2319000/1‑93 (MQ=255)
                        tACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAAc                                                           >  2:4246058/1‑93 (MQ=255)
                         aCGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACt                                                          >  2:7078607/1‑93 (MQ=255)
                          cGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTa                                                         >  2:3842877/1‑93 (MQ=255)
                          cGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTa                                                         <  1:5511543/93‑1 (MQ=255)
                          cGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTa                                                         <  1:1002675/93‑1 (MQ=255)
                           ggTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTAt                                                        <  2:6297313/93‑1 (MQ=255)
                            gTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGCGAACTATc                                                       >  2:2651706/1‑93 (MQ=255)
                            gTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATc                                                       <  1:503985/93‑1 (MQ=255)
                             tCGTGGACCGGGCGAAAACTCCGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATcc                                                      <  1:2005764/93‑1 (MQ=255)
                              cGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATccc                                                     >  2:753743/1‑93 (MQ=255)
                              cGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATccc                                                     <  2:1178411/93‑1 (MQ=255)
                               gTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCt                                                    >  1:1777511/1‑93 (MQ=255)
                               gTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCt                                                    <  1:7121201/93‑1 (MQ=255)
                                  gACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTcc                                                 >  2:3395001/1‑93 (MQ=255)
                                  gACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTcc                                                 >  1:3361679/1‑93 (MQ=255)
                                         cGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaa                                          >  1:4958049/1‑93 (MQ=255)
                                          gAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaac                                         >  2:6146869/1‑93 (MQ=255)
                                          gAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaac                                         <  2:3461467/93‑1 (MQ=255)
                                           aaaaCTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaaca                                        >  1:3436288/1‑93 (MQ=255)
                                            aaaCTACGCCGAGAGCCAGCAGGCTAACATGATCGATATCAGACTCACCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaacaa                                       <  2:414741/93‑1 (MQ=255)
                                            aaaCTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaacaa                                       <  2:2762141/93‑1 (MQ=255)
                                             aacaacGCCGACAGCCAGCACGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaacaac                                      <  2:6795810/93‑1 (MQ=255)
                                              aCTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaa                                          <  1:4327292/88‑1 (MQ=255)
                                               cTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGACCAACgg                                    >  2:1258721/1‑93 (MQ=255)
                                                 aCGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTa                                  <  2:7620478/93‑1 (MQ=255)
                                                   gCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAAc                                <  2:2048960/93‑1 (MQ=255)
                                                    ccGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAAcc                               <  2:822830/93‑1 (MQ=255)
                                                     cGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCg                              >  2:1038083/1‑93 (MQ=255)
                                                      gACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACACCGGTAACCGt                             >  1:518556/1‑93 (MQ=255)
                                                       aCAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTc                            <  2:7518918/93‑1 (MQ=255)
                                                         agccagcAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAg                          <  1:7564726/93‑1 (MQ=255)
                                                          gccagcAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGc                         >  2:2482544/1‑93 (MQ=255)
                                                          gccagcAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGCCAGc                         >  1:1634726/1‑93 (MQ=255)
                                                          gccagcAGGCTAACATCATCGATATCTGGCGCAGCACCGTCAATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACAGGAACAGTCAGc                         >  2:2949969/1‑93 (MQ=255)
                                                           ccagcAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCa                        <  1:7014090/93‑1 (MQ=255)
                                                           ccagcAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCa                        >  1:2663272/1‑93 (MQ=255)
                                                           ccagcAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCa                        <  1:4070960/93‑1 (MQ=255)
                                                            cagcagGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCAt                       <  2:5940808/93‑1 (MQ=255)
                                                            cagcagGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCAt                       >  2:5757282/1‑93 (MQ=255)
                                                            cagcagGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCAt                       <  1:1332125/93‑1 (MQ=255)
                                                            cagcagGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCAt                       <  2:1288947/93‑1 (MQ=255)
                                                             cgcagGCTAACACCATCGATATCTGTCGCAGCCCCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATg                      <  2:2215921/92‑1 (MQ=255)
                                                             agcagGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATg                      <  2:1488645/93‑1 (MQ=255)
                                                             agcagGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATg                      <  2:6728396/93‑1 (MQ=255)
                                                               cagTCTAACATTATCGATATCTGGCGCAGCCCCGTCGATGCCATGTTCGACAACTCTCCCCTCCCTCATAACAACCCTAACCGGCAGCATGTc                    <  2:6439177/93‑1 (MQ=255)
                                                               cagGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTc                    <  1:7204112/93‑1 (MQ=255)
                                                                atgCTAACATCGTCTATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTcc                   <  1:5904359/91‑1 (MQ=255)
                                                                agGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTcc                   <  2:7440010/93‑1 (MQ=255)
                                                                agGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTcc                   <  2:1110559/93‑1 (MQ=255)
                                                                 ggCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCCTCAGCATGTCCg                  >  1:6424646/1‑93 (MQ=255)
                                                                  gCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGc                 <  1:2379786/93‑1 (MQ=255)
                                                                  gCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGc                 >  2:5283121/1‑93 (MQ=255)
                                                                   cTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCt                >  1:4145860/1‑93 (MQ=255)
                                                                   cTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCt                <  2:1772649/93‑1 (MQ=255)
                                                                   cTAACATCATAGATATCTGACGCAGCACCGTCGACGCCATGTTCGAGTACTATCCCTTCCCGCCGCACCACGGTAACCGTCCGCATGTACGCt                >  1:3894279/1‑93 (MQ=255)
                                                                    tAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTg               <  1:1082298/93‑1 (MQ=255)
                                                                    tAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTg               >  1:6468789/1‑93 (MQ=255)
                                                                     aaCATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTgg              <  1:7052177/93‑1 (MQ=255)
                                                                     aaCATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTgg              >  1:375657/1‑93 (MQ=255)
                                                                      aCATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGa             <  1:7896829/93‑1 (MQ=255)
                                                                      aCATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGa             >  1:3224712/1‑93 (MQ=255)
                                                                        atcatcGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACg           >  2:432932/1‑93 (MQ=255)
                                                                        atcatcGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACg           <  2:3699291/93‑1 (MQ=255)
                                                                        atcatcGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACg           <  1:1807401/93‑1 (MQ=255)
                                                                         tcatcGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACgg          >  1:2938070/1‑93 (MQ=255)
                                                                         acatcGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACgg          <  2:4869706/92‑1 (MQ=255)
                                                                          catcGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGc         <  2:7649262/93‑1 (MQ=255)
                                                                           atcGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCCGGACGGCa        >  1:3082353/1‑93 (MQ=255)
                                                                            tcGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCAc       >  2:4617191/1‑93 (MQ=255)
                                                                               atatCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGa    >  1:858285/1‑93 (MQ=255)
                                                                               atatCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGa    <  1:2794934/93‑1 (MQ=255)
                                                                                          ||||                                                                                
GGCGAATACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCCAAGCCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACT  >  NC_000913/3222854‑3223027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGCGAATACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGC‑‑CAAGCCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACT  >  NC_000913/3222853‑3223027
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CGGCGAATACAATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGC‑‑Cgc                                                                                   >  SRR4302543.5358179/1‑92 (MQ=60)
           ATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGC                                                                        >  SRR4302543.3765386/1‑94 (MQ=60)
           ATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGC                                                                        >  SRR4302543.3765387/1‑94 (MQ=60)
           ATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGC                                                                        >  SRR4302543.3582926/1‑94 (MQ=60)
            TCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCC                                                                       <  SRR4302543.1532814/94‑1 (MQ=60)
             CAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCA                                                                      <  SRR4302543.3712/94‑1 (MQ=60)
             CAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCA                                                                      <  SRR4302543.113270/94‑1 (MQ=60)
              gacagccagcagtCGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCAT                                                                     <  SRR4302543.783657/81‑1 (MQ=60)
              AGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCAT                                                                     <  SRR4302543.7504177/94‑1 (MQ=60)
              AGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCAT                                                                     <  SRR4302543.4443602/94‑1 (MQ=60)
              AGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCAT                                                                     <  SRR4302543.649048/94‑1 (MQ=60)
                GTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGT                                                                   <  SRR4302543.1556879/94‑1 (MQ=60)
                GTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGT                                                                   >  SRR4302543.5633628/1‑94 (MQ=60)
                GTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGT                                                                   >  SRR4302543.2149451/1‑94 (MQ=60)
                 TGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTT                                                                  >  SRR4302543.4522329/1‑94 (MQ=60)
                 GGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTT                                                                  <  SRR4302543.3936744/94‑1 (MQ=60)
                  GGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTC                                                                 >  SRR4302543.4216753/1‑94 (MQ=60)
                   GCGTATTGCGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCG                                                                <  SRR4302543.4628863/94‑1 (MQ=60)
                   GCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCG                                                                >  SRR4302543.7244662/1‑94 (MQ=60)
                   GCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCG                                                                <  SRR4302543.14375/94‑1 (MQ=60)
                    CGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGA                                                               >  SRR4302543.6408029/1‑94 (MQ=60)
                     GTATTACGGTCGTGGACCGGGCTAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAG                                                              >  SRR4302543.1416971/1‑94 (MQ=60)
                     GTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAG                                                              >  SRR4302543.210108/1‑94 (MQ=60)
                      TATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGA                                                             >  SRR4302543.6323193/1‑94 (MQ=60)
                      TATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGA                                                             >  SRR4302543.4150227/1‑94 (MQ=60)
                      TATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGA                                                             >  SRR4302543.7081459/1‑94 (MQ=60)
                       ATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAA                                                            >  SRR4302543.5991678/1‑94 (MQ=60)
                       ATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAA                                                            <  SRR4302543.2129208/94‑1 (MQ=60)
                       ATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAA                                                            >  SRR4302543.892532/1‑94 (MQ=60)
                        TTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAAC                                                           >  SRR4302543.2340082/1‑94 (MQ=60)
                        TTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAAC                                                           <  SRR4302543.5735596/94‑1 (MQ=60)
                         TACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACT                                                          <  SRR4302543.7555026/94‑1 (MQ=60)
                         TACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACT                                                          <  SRR4302543.3212060/94‑1 (MQ=60)
                         TACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACT                                                          <  SRR4302543.2297264/94‑1 (MQ=60)
                          ACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTA                                                         <  SRR4302543.4645236/94‑1 (MQ=60)
                           CGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTAT                                                        <  SRR4302543.1011537/94‑1 (MQ=60)
                           CGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTAT                                                        <  SRR4302543.5561587/94‑1 (MQ=60)
                           CGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTAT                                                        <  SRR4302543.3010066/94‑1 (MQ=60)
                            ggttgtgtgCCGGGCGAAACCTACGCCGACAGGCAGCACGCTAACATAATCGATATCTGGCGC‑‑CGCCCCGTGGATGCCATGTTCGAGAACTATC                                                       <  SRR4302543.4899123/85‑1 (MQ=48)
                            GGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATC                                                       <  SRR4302543.272721/94‑1 (MQ=60)
                             GTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCC                                                      <  SRR4302543.508665/94‑1 (MQ=60)
                              TCGTGGACCGGGCGAAAACTCCGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCC                                                     <  SRR4302543.2023790/94‑1 (MQ=60)
                              TCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCC                                                     <  SRR4302543.6273191/94‑1 (MQ=60)
                               CGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCT                                                    <  SRR4302543.5608775/94‑1 (MQ=60)
                               CGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCT                                                    >  SRR4302543.1793390/1‑94 (MQ=60)
                               CGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCT                                                    <  SRR4302543.2072365/94‑1 (MQ=60)
                                GTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTT                                                   <  SRR4302543.2776818/94‑1 (MQ=60)
                                GTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTT                                                   <  SRR4302543.7190384/94‑1 (MQ=60)
                                  GGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCC                                                 >  SRR4302543.1645752/1‑94 (MQ=60)
                                  GGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCC                                                 >  SRR4302543.3391911/1‑94 (MQ=60)
                                  GGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCC                                                 <  SRR4302543.5286565/94‑1 (MQ=60)
                                  GGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCC                                                 <  SRR4302543.3530513/94‑1 (MQ=60)
                                     CCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGC                                              <  SRR4302543.597895/94‑1 (MQ=60)
                                     CCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGC                                              >  SRR4302543.6487645/1‑94 (MQ=60)
                                      CGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCA                                             <  SRR4302543.2589224/94‑1 (MQ=60)
                                       GGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAG                                            <  SRR4302543.3538122/94‑1 (MQ=60)
                                       GGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAG                                            >  SRR4302543.2395507/1‑94 (MQ=60)
                                       GGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAG                                            >  SRR4302543.1594473/1‑94 (MQ=60)
                                         GCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAA                                          >  SRR4302543.5003186/1‑94 (MQ=60)
                                          ctctAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAAC                                         <  SRR4302543.4366570/90‑1 (MQ=60)
                                           GAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACA                                        >  SRR4302543.1207570/1‑94 (MQ=60)
                                           GAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACA                                        >  SRR4302543.5180194/1‑94 (MQ=60)
                                           GAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACA                                        >  SRR4302543.5671337/1‑94 (MQ=60)
                                           GAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACA                                        >  SRR4302543.7152120/1‑94 (MQ=60)
                                           GAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACA                                        >  SRR4302543.6428555/1‑94 (MQ=60)
                                           GAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACA                                        >  SRR4302543.3467045/1‑94 (MQ=60)
                                           GAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACA                                        >  SRR4302543.807284/1‑94 (MQ=60)
                                             AAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAAC                                      <  SRR4302543.2558597/94‑1 (MQ=60)
                                             AAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAAC                                      >  SRR4302543.152690/1‑94 (MQ=60)
                                             AAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAAC                                      >  SRR4302543.1734492/1‑94 (MQ=60)
                                             AAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAAC                                      <  SRR4302543.7659982/94‑1 (MQ=60)
                                              AACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACG                                     <  SRR4302543.3807740/94‑1 (MQ=60)
                                               ACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGG                                    <  SRR4302543.5458546/94‑1 (MQ=60)
                                               ACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGG                                    <  SRR4302543.5326999/94‑1 (MQ=60)
                                               ACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGG                                    <  SRR4302543.3414565/94‑1 (MQ=60)
                                                   CGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAAC                                <  SRR4302543.5860808/94‑1 (MQ=60)
                                                    GCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACC                               <  SRR4302543.6885758/94‑1 (MQ=60)
                                                    GCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACC                               <  SRR4302543.3135185/94‑1 (MQ=60)
                                                      CGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACACCGGTAACCGT                             >  SRR4302543.523371/1‑94 (MQ=60)
                                                      CGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGT                             <  SRR4302543.6323666/94‑1 (MQ=60)
                                                          AGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGC                         <  SRR4302543.7636718/94‑1 (MQ=60)
                                                          AGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGCCAGC                         >  SRR4302543.1649307/1‑94 (MQ=60)
                                                           GCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCA                        >  SRR4302543.2687418/1‑94 (MQ=60)
                                                           ACCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCA                        <  SRR4302543.2286423/94‑1 (MQ=60)
                                                            CCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCAT                       <  SRR4302543.4107425/94‑1 (MQ=60)
                                                            CCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCAT                       <  SRR4302543.7081045/94‑1 (MQ=60)
                                                             CAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATG                      >  SRR4302543.5743149/1‑94 (MQ=60)
                                                             CAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATG                      <  SRR4302543.1343996/94‑1 (MQ=60)
                                                               GCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTC                    <  SRR4302543.6293730/94‑1 (MQ=60)
                                                                CAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCC                   <  SRR4302543.7273959/94‑1 (MQ=60)
                                                                CAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCC                   >  SRR4302543.7623229/1‑94 (MQ=60)
                                                                CAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCC                   <  SRR4302543.1794375/94‑1 (MQ=60)
                                                                CAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCC                   <  SRR4302543.7915127/94‑1 (MQ=60)
                                                                CAGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCC                   <  SRR4302543.1985955/94‑1 (MQ=60)
                                                                 ATGCTAACATCGTCTATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCG                  <  SRR4302543.5958246/94‑1 (MQ=60)
                                                                 AGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCG                  >  SRR4302543.7579765/1‑94 (MQ=60)
                                                                 AGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCG                  <  SRR4302543.1812165/94‑1 (MQ=60)
                                                                 AGGCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCCTCAGCATGTCCG                  >  SRR4302543.6485185/1‑94 (MQ=60)
                                                                   GCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCT                <  SRR4302543.2401339/94‑1 (MQ=60)
                                                                   GCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCT                <  SRR4302543.7234427/94‑1 (MQ=60)
                                                                   GCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCT                <  SRR4302543.3390800/94‑1 (MQ=60)
                                                                   GCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCT                >  SRR4302543.3928663/1‑94 (MQ=60)
                                                                   GCTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCT                >  SRR4302543.4183324/1‑94 (MQ=60)
                                                                    CTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTG               >  SRR4302543.4358291/1‑94 (MQ=60)
                                                                    CTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTG               >  SRR4302543.1200197/1‑94 (MQ=60)
                                                                    CTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTG               >  SRR4302543.6529731/1‑94 (MQ=60)
                                                                    CTAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTG               <  SRR4302543.3051478/94‑1 (MQ=60)
                                                                     TAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGG              >  SRR4302543.379116/1‑94 (MQ=60)
                                                                     TAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGG              >  SRR4302543.1952125/1‑94 (MQ=60)
                                                                     TAACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGG              <  SRR4302543.1092026/94‑1 (MQ=60)
                                                                      AACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGA             <  SRR4302543.5371946/94‑1 (MQ=60)
                                                                      AACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGA             >  SRR4302543.3253860/1‑94 (MQ=60)
                                                                      AACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGA             <  SRR4302543.7120608/94‑1 (MQ=60)
                                                                       ACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGAC            <  SRR4302543.7169481/94‑1 (MQ=60)
                                                                       ACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGAC            <  SRR4302543.7971075/94‑1 (MQ=60)
                                                                       ACATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGAC            <  SRR4302543.7971074/94‑1 (MQ=60)
                                                                        CATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACG           <  SRR4302543.3431766/94‑1 (MQ=60)
                                                                         ATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGG          >  SRR4302543.7050079/1‑94 (MQ=60)
                                                                         ATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGG          <  SRR4302543.1823581/94‑1 (MQ=60)
                                                                         ATCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGG          >  SRR4302543.2964757/1‑94 (MQ=60)
                                                                          TCATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGC         >  SRR4302543.1078865/1‑94 (MQ=60)
                                                                           CATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCA        >  SRR4302543.2863269/1‑94 (MQ=60)
                                                                           CATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCCGGACGGCA        >  SRR4302543.3110071/1‑94 (MQ=60)
                                                                            ATCGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCAC       <  SRR4302543.4783874/94‑1 (MQ=60)
                                                                              CGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTG     <  SRR4302543.5071780/94‑1 (MQ=60)
                                                                              CGATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTG     >  SRR4302543.4663788/1‑94 (MQ=60)
                                                                              CGATATCTGGCGC‑‑CGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTG     <  SRR4302543.5734021/94‑1 (MQ=60)
                                                                               GATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGCCGGCACTGC    >  SRR4302543.1132870/1‑94 (MQ=60)
                                                                               GATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGA    >  SRR4302543.865930/1‑94 (MQ=60)
                                                                               GATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGA    >  SRR4302543.865931/1‑94 (MQ=60)
                                                                                ATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGAC   >  SRR4302543.3847261/1‑94 (MQ=60)
                                                                                ATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGAC   <  SRR4302543.2820473/94‑1 (MQ=60)
                                                                                ATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGAC   <  SRR4302543.1292030/94‑1 (MQ=60)
                                                                                ATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGAC   >  SRR4302543.930969/1‑94 (MQ=60)
                                                                                ATATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTAGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGAC   >  SRR4302543.5060841/1‑94 (MQ=60)
                                                                                 TATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACT  >  SRR4302543.3784871/1‑94 (MQ=60)
                                                                                 TATCTGGCGCAGCA‑‑CCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACT  <  SRR4302543.359024/94‑1 (MQ=60)
                                                                                             |                                                                                   
CGGCGAATACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGC‑‑CAAGCCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACT  >  NC_000913/3222853‑3223027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑