Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,233,455 T→C D21G (GAC→GGC)  b3084 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,233,4550TC100.0% 645.7 / NA 205D21G (GAC→GGC) b3084hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (114/91);  total (114/91)

Only 100 of 212 total aligned reads displayed.

AGGATCAACACCGGGCCGCGGATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGTCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCCTGAATGATAAAATTTCGAC  >  NC_000913/3233365‑3233544
                                                                                          |                                                                                         
aGGATCAACACCGGGCCGCGGATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCa                                                                                         <  2:3689532/93‑1 (MQ=255)
 ggATCAACACCGGGCCGCGGATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCAt                                                                                        >  2:4351796/1‑93 (MQ=255)
  gATCAACACCGGGCCGCGGATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATc                                                                                       <  1:520560/93‑1 (MQ=255)
    tCAACACCGGGCCGCGGATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCg                                                                                     <  2:3708511/93‑1 (MQ=255)
      aaCACCGGGCCGCGGATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTc                                                                                   <  2:495482/93‑1 (MQ=255)
      aaCACCGGGCCGCGGATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTc                                                                                   >  2:3999322/1‑93 (MQ=255)
       acacCGGGCCGCGGATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCg                                                                                  >  1:3442930/1‑93 (MQ=255)
       acacCGGGCCGCGGATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCg                                                                                  >  1:2352125/1‑93 (MQ=255)
        cacCGGGCCGCGGATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGc                                                                                 <  1:3068655/93‑1 (MQ=255)
        cacCGGGCCGCGGATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGc                                                                                 <  2:1129511/93‑1 (MQ=255)
         acCGGGCCGCGGATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGcc                                                                                >  1:4310365/1‑93 (MQ=255)
         acCGGGCCGCGGATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGcc                                                                                >  2:2172440/1‑93 (MQ=255)
            gggCCGCGGATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGa                                                                             >  1:853925/1‑93 (MQ=255)
             ggCCGCGGATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGaa                                                                            <  2:2646150/93‑1 (MQ=255)
             gcccGGGGATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGaa                                                                            <  2:3220947/91‑1 (MQ=255)
               ccGCGGATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGaaaa                                                                          <  2:4299377/93‑1 (MQ=255)
                cgcgGATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAAc                                                                         <  2:4059636/93‑1 (MQ=255)
                cgcgGATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAAc                                                                         <  1:4510799/93‑1 (MQ=255)
                   ggATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGtt                                                                      <  2:1483565/93‑1 (MQ=255)
                   ggATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGtt                                                                      >  1:3130164/1‑93 (MQ=255)
                    gATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTg                                                                     >  1:662002/1‑93 (MQ=255)
                     aTTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGt                                                                    >  2:4081431/1‑93 (MQ=255)
                     aTTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGt                                                                    <  2:2849369/93‑1 (MQ=255)
                      tttCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTa                                                                   >  2:4224535/1‑93 (MQ=255)
                      tttCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTa                                                                   >  2:3885671/1‑93 (MQ=255)
                       ttCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAg                                                                  <  2:1757201/93‑1 (MQ=255)
                        tCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAgt                                                                 <  2:2468189/93‑1 (MQ=255)
                          tgtgTCGTCCAGCTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAgtgt                                                               <  2:1091683/93‑1 (MQ=255)
                          tgtgTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAAAGTTGTAgtgt                                                               >  2:1389790/1‑93 (MQ=255)
                            tgtCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCa                                                             >  2:4619138/1‑93 (MQ=255)
                             gtcgtcCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAg                                                            <  1:3516931/93‑1 (MQ=255)
                               cgtcCGACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTg                                                          >  2:1289027/1‑93 (MQ=255)
                                gtcCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGa                                                         >  1:3674949/1‑93 (MQ=255)
                                 tcCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGaa                                                        >  1:1822623/1‑93 (MQ=255)
                                   cAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACg                                                      >  1:2359109/1‑93 (MQ=255)
                                    aaCTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACgg                                                     >  1:4415220/1‑93 (MQ=255)
                                     acggTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGa                                                    <  2:1739877/90‑1 (MQ=255)
                                     aCTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGa                                                    >  1:2611514/1‑93 (MQ=255)
                                      cTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGaa                                                   <  1:4153267/93‑1 (MQ=255)
                                      cTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGaa                                                   >  1:2545588/1‑93 (MQ=255)
                                       tGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGaaa                                                  >  2:3027893/1‑93 (MQ=255)
                                       tGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGaaa                                                  <  2:905410/93‑1 (MQ=255)
                                        gTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAAc                                                 <  2:1547030/93‑1 (MQ=255)
                                          tGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCg                                               >  1:3805287/1‑93 (MQ=255)
                                           gcagcaAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGt                                              >  2:259544/1‑93 (MQ=255)
                                            cagcaAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGtt                                             >  2:3780837/1‑93 (MQ=255)
                                            cagcaAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGtt                                             >  2:576725/1‑93 (MQ=255)
                                             agcaAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTg                                            >  2:3569080/1‑93 (MQ=255)
                                             agcaAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTg                                            >  1:1882344/1‑93 (MQ=255)
                                             agcaAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGGTAACGCCCTCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGCTg                                            >  1:1054870/1‑93 (MQ=255)
                                               caAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTc                                          <  1:2927744/93‑1 (MQ=255)
                                                 aaTATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTa                                        >  2:3454290/1‑93 (MQ=255)
                                                 aaTATCCACCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTa                                        <  2:223179/93‑1 (MQ=255)
                                                   tatTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAg                                      <  1:3528324/93‑1 (MQ=255)
                                                   tatTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAg                                      >  2:1985724/1‑93 (MQ=255)
                                                    atTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAgg                                     >  2:2782031/1‑92 (MQ=255)
                                                    atTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGt                                     <  2:309385/93‑1 (MQ=255)
                                                     ttCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTg                                    >  2:1078579/1‑93 (MQ=255)
                                                      tCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTCTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTgg                                   >  1:2232977/1‑93 (MQ=255)
                                                       cATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGc                                  <  2:2157616/93‑1 (MQ=255)
                                                       cATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGc                                  <  1:3286947/93‑1 (MQ=255)
                                                        aTCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCt                                 <  1:715471/93‑1 (MQ=255)
                                                        aTCAGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGGGAACGGAAACCGTTGTCTAAGTGGCt                                 >  2:2115572/1‑93 (MQ=255)
                                                         tCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTc                                >  2:631744/1‑93 (MQ=255)
                                                           cgccgcTTCCCACGCCTGTAGCTGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCAt                              <  2:2864131/93‑1 (MQ=255)
                                                           cgccgcTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCAt                              <  2:3808922/93‑1 (MQ=255)
                                                            gccgcTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATc                             <  1:242779/93‑1 (MQ=255)
                                                             ccgcTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCg                            >  2:2285547/1‑93 (MQ=255)
                                                               gcTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGc                          >  2:1163158/1‑93 (MQ=255)
                                                               gcTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGc                          <  1:4642381/93‑1 (MQ=255)
                                                                cTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGcc                         >  2:1586285/1‑93 (MQ=255)
                                                                cTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGcc                         >  1:1367631/1‑93 (MQ=255)
                                                                 ttCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGccc                        >  1:2021057/1‑93 (MQ=255)
                                                                 ttCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGccc                        <  1:3108439/93‑1 (MQ=255)
                                                                 ttCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGccc                        <  1:88319/93‑1 (MQ=255)
                                                                   cccACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCcctc                      <  1:3588058/93‑1 (MQ=255)
                                                                   cccACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCcctc                      <  1:25009/93‑1 (MQ=255)
                                                                    ccACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCcctcc                     >  1:2095762/1‑93 (MQ=255)
                                                                    ccACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCcctcc                     >  1:4061216/1‑93 (MQ=255)
                                                                      aCGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCCTg                   >  2:3531596/1‑93 (MQ=255)
                                                                         ccTGTAGCAGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAACCCGTTGTCTAAGTGGCTCATCGGCCCTCCTGAAt                <  1:922442/93‑1 (MQ=255)
                                                                          cTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCCTGAATg               >  1:1026051/1‑93 (MQ=255)
                                                                          cTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCCTGAATg               >  2:2977205/1‑93 (MQ=255)
                                                                          cTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCCTGAATg               >  1:55568/1‑93 (MQ=255)
                                                                           tGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCCTGAATGa              <  1:1728974/93‑1 (MQ=255)
                                                                           tGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCCTGAATGa              >  1:3293677/1‑93 (MQ=255)
                                                                           tGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCCTGAATGa              <  1:111580/93‑1 (MQ=255)
                                                                            gTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCCTGAATGAt             <  1:3720634/93‑1 (MQ=255)
                                                                              aGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCCTGAATGATaa           <  1:3725929/93‑1 (MQ=255)
                                                                              aGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCCTGAATGATaa           <  2:537098/93‑1 (MQ=255)
                                                                                 gggTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCCTGAATGATAAAAt        <  1:3029136/93‑1 (MQ=255)
                                                                                 gggTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCCTGAATGATAAAAt        >  2:4422678/1‑93 (MQ=255)
                                                                                  ggTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCCTGAATGATAAAAtt       >  2:4464612/1‑93 (MQ=255)
                                                                                    ttAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGNCCTCCTGAATGATAAAATTTc     >  1:2164147/1‑93 (MQ=255)
                                                                                      aaCGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCCTGAATGATAAAATTTCGa   >  2:1870666/1‑93 (MQ=255)
                                                                                      aaCGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCCTGAATGATAAAATTTCGa   <  2:3969508/93‑1 (MQ=255)
                                                                                      aaCGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCCTGAATGATAAAATTTCGa   >  2:78138/1‑93 (MQ=255)
                                                                                       aCGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCCTGAATGATAAAATTTCGAc  <  2:2338796/93‑1 (MQ=255)
                                                                                       aCGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCCTGAATGATAAAATTTCGAc  <  1:3174859/93‑1 (MQ=255)
                                                                                       aCGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCCTGAATGATAAAATTTCGAc  >  2:745419/1‑93 (MQ=255)
                                                                                          |                                                                                         
AGGATCAACACCGGGCCGCGGATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGTCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCCTGAATGATAAAATTTCGAC  >  NC_000913/3233365‑3233544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTCAGGATCAACACCGGGCCGCGGATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGTCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCCTGAATGATAAAATTTCGACATTG  >  NC_000913/3233362‑3233548
                                                                                             |                                                                                             
TTCAGGATCAACACCGGGCCGCGGATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGC                                                                                               >  SRR4302535.3672526/1‑94 (MQ=60)
   AGGATCAACACCGGGCCGCGGATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCAT                                                                                            >  SRR4302535.3746639/1‑94 (MQ=60)
   AGGATCAACACCGGGCCGCGGATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCAT                                                                                            >  SRR4302535.4189865/1‑94 (MQ=60)
     GATCAACACCGGGCCGCGGATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCC                                                                                          <  SRR4302535.524199/94‑1 (MQ=60)
      ATCAACACCGGGCCGCGGATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCG                                                                                         <  SRR4302535.1104514/94‑1 (MQ=60)
      ATCAACACCGGGCCGCGGATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCG                                                                                         <  SRR4302535.2238467/94‑1 (MQ=60)
         AACACCGGGCCGCGGATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCG                                                                                      >  SRR4302535.2368985/1‑94 (MQ=60)
         AACACCGGGCCGCGGATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCG                                                                                      >  SRR4302535.3466872/1‑94 (MQ=60)
         AACACCGGGCCGCGGATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCG                                                                                      >  SRR4302535.3727411/1‑94 (MQ=60)
         AACACCGGGCCGCGGATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCG                                                                                      >  SRR4302535.4089304/1‑94 (MQ=60)
           CACCGGGCCGCGGATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCC                                                                                    >  SRR4302535.4339849/1‑94 (MQ=60)
           CACCGGGCCGCGGATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCC                                                                                    <  SRR4302535.3090090/94‑1 (MQ=60)
            ACCGGGCCGCGGATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCG                                                                                   >  SRR4302535.791095/1‑94 (MQ=60)
            ACCGGGCCGCGGATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCG                                                                                   <  SRR4302535.670546/94‑1 (MQ=60)
              CGGGCCGCGGATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGA                                                                                 >  SRR4302535.859939/1‑94 (MQ=60)
                 GCCGCGGATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAA                                                                              >  SRR4302535.974390/1‑94 (MQ=60)
                  CCGCGGATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAAC                                                                             >  SRR4302535.1553535/1‑94 (MQ=60)
                  CCGCGGATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAAC                                                                             <  SRR4302535.4703703/94‑1 (MQ=60)
                   CGCGGATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACG                                                                            <  SRR4302535.4541742/94‑1 (MQ=60)
                     CGGATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTT                                                                          >  SRR4302535.3151965/1‑94 (MQ=60)
                      GGATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTG                                                                         >  SRR4302535.666622/1‑94 (MQ=60)
                      GGATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTG                                                                         >  SRR4302535.528309/1‑94 (MQ=60)
                      GGATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTG                                                                         <  SRR4302535.1794373/94‑1 (MQ=60)
                        ATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTA                                                                       >  SRR4302535.3536405/1‑94 (MQ=60)
                        ATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTA                                                                       <  SRR4302535.2154208/94‑1 (MQ=60)
                          TTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGT                                                                     <  SRR4302535.4102092/94‑1 (MQ=60)
                            CTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGNCTGTAGCGGGTTAACGCCATCNNTCNCCGGAAAACGNTNNAGNGT                                                                   >  SRR4302535.3538978/1‑94 (MQ=60)
                            CTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGT                                                                   >  SRR4302535.135935/1‑94 (MQ=60)
                              GTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTtg                                                                 >  SRR4302535.904025/1‑92 (MQ=60)
                               TGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAG                                                                <  SRR4302535.2608315/94‑1 (MQ=60)
                                GTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGT                                                               <  SRR4302535.3541365/94‑1 (MQ=60)
                                  CGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGA                                                             >  SRR4302535.3700393/1‑94 (MQ=60)
                                  CGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGA                                                             >  SRR4302535.273606/1‑94 (MQ=60)
                                   ATCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAA                                                            >  SRR4302535.1835789/1‑94 (MQ=60)
                                    tctaaatgttcgAGCAAATATTCTACCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAAC                                                           <  SRR4302535.2468411/82‑1 (MQ=60)
                                     CCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACG                                                          >  SRR4302535.2376037/1‑94 (MQ=60)
                                      CAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGG                                                         >  SRR4302535.2006759/1‑94 (MQ=60)
                                      CAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGG                                                         >  SRR4302535.4445437/1‑94 (MQ=60)
                                       AACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGA                                                        >  SRR4302535.2630249/1‑94 (MQ=60)
                                        ACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAA                                                       >  SRR4302535.2563913/1‑94 (MQ=60)
                                         CTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAA                                                      >  SRR4302535.3812908/1‑94 (MQ=60)
                                         CTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAA                                                      <  SRR4302535.4181706/94‑1 (MQ=60)
                                          TGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAAC                                                     <  SRR4302535.3169483/94‑1 (MQ=60)
                                          TGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAAC                                                     >  SRR4302535.3228440/1‑94 (MQ=60)
                                           GTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACC                                                    >  SRR4302535.186369/1‑94 (MQ=60)
                                            TTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCG                                                   >  SRR4302535.3080025/1‑94 (MQ=60)
                                            TTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCG                                                   >  SRR4302535.3831516/1‑94 (MQ=60)
                                            TTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCG                                                   <  SRR4302535.3348331/94‑1 (MQ=60)
                                               CAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTG                                                >  SRR4302535.1895911/1‑94 (MQ=60)
                                               CAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGGTAACGCCCTCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGCTG                                                >  SRR4302535.1062371/1‑94 (MQ=60)
                                                  CAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCT                                             <  SRR4302535.2948377/94‑1 (MQ=60)
                                                     ATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAG                                          >  SRR4302535.3316570/1‑94 (MQ=60)
                                                     ATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAG                                          >  SRR4302535.2395880/1‑94 (MQ=60)
                                                      TATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGT                                         <  SRR4302535.3552825/94‑1 (MQ=60)
                                                        TTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGG                                       >  SRR4302535.582824/1‑94 (MQ=60)
                                                        TTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTCTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGG                                       >  SRR4302535.2248856/1‑94 (MQ=60)
                                                         TCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGC                                      >  SRR4302535.4441744/1‑94 (MQ=60)
                                                          CATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCT                                     <  SRR4302535.3309798/94‑1 (MQ=60)
                                                          CATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCT                                     >  SRR4302535.337113/1‑94 (MQ=60)
                                                           ATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTC                                    <  SRR4302535.720496/94‑1 (MQ=60)
                                                             CCGCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCAT                                  >  SRR4302535.3693744/1‑94 (MQ=60)
                                                               GCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCG                                <  SRR4302535.244441/94‑1 (MQ=60)
                                                               GCCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCG                                <  SRR4302535.2983974/94‑1 (MQ=60)
                                                                CCGCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGG                               >  SRR4302535.461151/1‑94 (MQ=60)
                                                                  GCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAACCCGTTGTCTAAGTGGCTCATCGGCC                             <  SRR4302535.2069931/94‑1 (MQ=60)
                                                                  GCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCC                             <  SRR4302535.4673848/94‑1 (MQ=60)
                                                                  GCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCC                             >  SRR4302535.887400/1‑94 (MQ=60)
                                                                  GCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCC                             >  SRR4302535.1377406/1‑94 (MQ=60)
                                                                  GCTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCC                             >  SRR4302535.2500654/1‑94 (MQ=60)
                                                                   CTTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCC                            >  SRR4302535.2035626/1‑94 (MQ=60)
                                                                    TTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCT                           <  SRR4302535.3130079/94‑1 (MQ=60)
                                                                    TTCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCT                           <  SRR4302535.88942/94‑1 (MQ=60)
                                                                     TCCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTC                          >  SRR4302535.2153476/1‑94 (MQ=60)
                                                                      CCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCC                         <  SRR4302535.3612947/94‑1 (MQ=60)
                                                                      CCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCC                         <  SRR4302535.25202/94‑1 (MQ=60)
                                                                      CCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCC                         >  SRR4302535.2110876/1‑94 (MQ=60)
                                                                      CCCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCC                         >  SRR4302535.4089014/1‑94 (MQ=60)
                                                                       CCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCCT                        <  SRR4302535.3600054/94‑1 (MQ=60)
                                                                       CCACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCCT                        >  SRR4302535.3130156/1‑94 (MQ=60)
                                                                        CACGCCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCCTG                       <  SRR4302535.3545016/94‑1 (MQ=60)
                                                                            CCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCCTGAATG                   >  SRR4302535.55975/1‑94 (MQ=60)
                                                                            CCTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCCTGAATG                   >  SRR4302535.1033338/1‑94 (MQ=60)
                                                                            CCTGTAGCAGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAACCCGTTGTCTAAGTGGCTCATCGGCCCTCCTGAATG                   <  SRR4302535.928980/94‑1 (MQ=60)
                                                                             CTGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCCTGAATGA                  >  SRR4302535.3316574/1‑94 (MQ=60)
                                                                              TGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCCTGAATGAT                 <  SRR4302535.112365/94‑1 (MQ=60)
                                                                              TGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCCTGAATGAT                 >  SRR4302535.711736/1‑94 (MQ=60)
                                                                              TGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCCTGAATGAT                 <  SRR4302535.1741469/94‑1 (MQ=60)
                                                                              TGTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCCTGAATGAT                 <  SRR4302535.3844205/94‑1 (MQ=60)
                                                                               GTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCCTGAATGATA                <  SRR4302535.3746411/94‑1 (MQ=60)
                                                                               GTAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCCTGAATGATA                >  SRR4302535.3125800/1‑94 (MQ=60)
                                                                                TAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCCTGAATGATAG               >  SRR4302535.4531400/1‑94 (MQ=60)
                                                                                TAGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCCTGAATGATAA               >  SRR4302535.1052930/1‑94 (MQ=60)
                                                                                 AGCGGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCCTGAATGATAAA              <  SRR4302535.3751743/94‑1 (MQ=60)
                                                                                    GGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGCACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCCTGCATGATAAAATT           >  SRR4302535.2560986/1‑94 (MQ=60)
                                                                                    GGGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCCTGAATGATAAAATT           <  SRR4302535.3050339/94‑1 (MQ=60)
                                                                                     GGTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCCTGAATGATAAAATTT          >  SRR4302535.2936896/1‑94 (MQ=60)
                                                                                      GTTAACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGNCCTCCTGAATGATAAAATTTC         >  SRR4302535.2179668/1‑94 (MQ=60)
                                                                                         AACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCCTGAATGATAAAATTTCGAC      <  SRR4302535.2276823/94‑1 (MQ=60)
                                                                                          ACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCCTGAATGATAAAATTTCGACA     <  SRR4302535.2375939/94‑1 (MQ=60)
                                                                                          ACGCCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCCTGAATGATAAAATTTCGACA     <  SRR4302535.3196830/94‑1 (MQ=60)
                                                                                           CGNCNCNCNTCGCCGGAAAANNNTGTANTGTCAGTGAACGGANACCGTTGTCTAAGTGGCTCATCGGCCCTCCTGAATGATAAAATTTCGACAT    <  SRR4302535.1682008/94‑1 (MQ=60)
                                                                                             CCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCCTGAATGATAAAATTTCGACATTG  >  SRR4302535.2589082/1‑94 (MQ=60)
                                                                                             |                                                                                             
TTCAGGATCAACACCGGGCCGCGGATTTCTGTGTCGTCCAACTGTTGCAGCAAATATTCATCCGCCGCTTCCCACGCCTGTAGCGGGTTAACGTCATCCGTCGCCGGAAAACGTTGTAGTGTCAGTGAACGGAAACCGTTGTCTAAGTGGCTCATCGGCCCTCCTGAATGATAAAATTTCGACATTG  >  NC_000913/3233362‑3233548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑