Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,237,247 +C intergenic (+60/‑337) ygjT → / → ygjU putative transport protein/putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,237,2451.C100.0% 481.7 / NA 156intergenic (+58/‑339)ygjT/ygjUputative transport protein/putative transport protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (86/70);  total (86/70)

Only 100 of 178 total aligned reads displayed.

ATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑T‑CCTCT‑CCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGG  >  NC_000913/3237156‑3237332
                                                                                            |                                                                                       
aTTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTc‑tccctct‑cc                                                                                       <  1:72246/93‑1 (MQ=255)
aTTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTc‑tccctct‑cc                                                                                       >  2:2816037/1‑93 (MQ=255)
 ttATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTc‑tccctct‑ccc                                                                                      >  1:539296/1‑92 (MQ=255)
 ttATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTc‑tccctct‑ccc                                                                                      >  2:1783988/1‑92 (MQ=255)
    tCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTc‑tccctct‑cccttc                                                                                   >  1:1045885/1‑89 (MQ=255)
    tCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTc‑tccctct‑cccttc                                                                                   >  1:3615634/1‑89 (MQ=255)
      ggCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCa                                                                                  >  1:3253399/1‑93 (MQ=255)
      ggCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCa                                                                                  <  2:3214581/93‑1 (MQ=255)
       gCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAg                                                                                 <  1:1915982/93‑1 (MQ=255)
       gCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAg                                                                                 <  1:668071/93‑1 (MQ=255)
       gCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAg                                                                                 <  2:4411050/93‑1 (MQ=255)
       gCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAg                                                                                 <  1:3454267/93‑1 (MQ=255)
          tGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGga                                                                              <  1:2134429/93‑1 (MQ=255)
          tGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGga                                                                              >  2:2479540/1‑93 (MQ=255)
          tGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGga                                                                              <  2:2757400/93‑1 (MQ=255)
          tGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGga                                                                              <  1:3794452/93‑1 (MQ=255)
          tGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGga                                                                              <  1:4614689/93‑1 (MQ=255)
          tGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGga                                                                              >  2:3583448/1‑93 (MQ=255)
          tGATAAGCAGCGGGGGGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGga                                                                              >  2:2631416/1‑93 (MQ=255)
           gATAAGCAGCGGGGTGTATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGgag                                                                             <  2:2555723/93‑1 (MQ=255)
           gATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGgag                                                                             <  1:117948/93‑1 (MQ=255)
            aTAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGgagg                                                                            <  1:4406982/93‑2 (MQ=255)
            aTAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGgagg                                                                            >  2:1329748/1‑92 (MQ=255)
            aTAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGgagg                                                                            <  1:300510/93‑2 (MQ=255)
            aTAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGgagg                                                                            <  2:3195030/93‑2 (MQ=255)
            aTAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGgagg                                                                            <  2:298148/93‑2 (MQ=255)
             tAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTCCCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGgaggg                                                                           <  2:1790270/93‑3 (MQ=255)
              aaGCAGCGGGGTGGTTAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCGCCGTTTTCAC‑CCTCTCCCTTCCAGGGAgggg                                                                          <  1:4019498/93‑1 (MQ=255)
                gcagcGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGt                                                                        <  2:2339310/93‑1 (MQ=255)
                gcagcGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGt                                                                        <  2:2805972/93‑1 (MQ=255)
                 cagcGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTGACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTc                                                                       >  1:2685863/1‑93 (MQ=255)
                     ggggTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCgggg                                                                   <  1:3957961/93‑1 (MQ=255)
                       ggTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTg                                                                 >  2:2454508/1‑93 (MQ=255)
                        gTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGa                                                                >  1:3013331/1‑93 (MQ=255)
                        gTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGa                                                                <  1:4105053/93‑1 (MQ=255)
                         tGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAg                                                               <  1:864956/93‑1 (MQ=255)
                         tGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAg                                                               <  2:971537/93‑1 (MQ=255)
                          ggATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCATCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAgg                                                              <  1:2512235/93‑1 (MQ=255)
                           gATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAggg                                                             <  1:1073197/93‑1 (MQ=255)
                             tAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTa                                                           >  1:2069932/1‑93 (MQ=38)
                             tAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGGGAGGGTa                                                           >  1:3293078/1‑93 (MQ=255)
                               aTTTTTAATCTGCCTAAGCCGTGTACTCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAAt                                                         <  2:3795048/93‑1 (MQ=255)
                                tttttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAAtt                                                        <  1:724597/93‑1 (MQ=255)
                                 ttttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAAttt                                                       >  2:3661331/1‑93 (MQ=38)
                                  tttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAAtttt                                                      <  1:4191695/93‑1 (MQ=255)
                                  tttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAAtttt                                                      >  2:102199/1‑93 (MQ=38)
                                  tttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAAtttt                                                      >  2:568835/1‑93 (MQ=38)
                                    tAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTc                                                    <  1:3418323/93‑1 (MQ=38)
                                    tAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTc                                                    <  1:2325028/93‑1 (MQ=38)
                                     aaTCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCg                                                   >  1:1579819/1‑93 (MQ=37)
                                      aTCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGc                                                  >  1:1484855/1‑93 (MQ=38)
                                      aTCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGc                                                  >  2:477637/1‑93 (MQ=38)
                                       tCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCa                                                 <  2:4468614/93‑1 (MQ=37)
                                       tCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCa                                                 >  2:1599250/1‑93 (MQ=38)
                                       tCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCa                                                 <  2:342694/93‑1 (MQ=37)
                                         tGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCAcc                                               >  1:2601423/1‑93 (MQ=38)
                                         tGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCAcc                                               >  2:370212/1‑93 (MQ=38)
                                         tGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCAcc                                               >  1:4313680/1‑93 (MQ=38)
                                             tAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATg                                           >  1:764676/1‑93 (MQ=38)
                                             tAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATg                                           >  2:2888891/1‑93 (MQ=38)
                                             tAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATg                                           >  1:2037199/1‑93 (MQ=38)
                                             tAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATg                                           >  1:3151706/1‑93 (MQ=38)
                                                gCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTg                                        <  2:2922445/93‑1 (MQ=38)
                                                   gtgtACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGcc                                     >  1:787263/1‑93 (MQ=37)
                                                    tgtACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCt                                    >  1:3846761/1‑93 (MQ=37)
                                                    tgtACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCt                                    >  2:2034739/1‑93 (MQ=37)
                                                      tACCCTGTCATCAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGt                                  <  2:2487338/93‑1 (MQ=25)
                                                       tcccTGTCATTAACATGAGCACCGTTTTCGC‑CCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGtt                                 <  2:3303800/92‑1 (MQ=37)
                                                       aCCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGtt                                 >  1:4461096/1‑93 (MQ=37)
                                                       aCCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGtt                                 >  1:3348101/1‑93 (MQ=37)
                                                       aCCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGtt                                 <  2:3339304/93‑1 (MQ=38)
                                                       aCCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGtt                                 >  1:1614813/1‑93 (MQ=37)
                                                        cccTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTc                                >  1:2650788/1‑93 (MQ=37)
                                                          cTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTgcc                              >  1:3703481/1‑93 (MQ=25)
                                                          cTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTccc                              >  1:212099/1‑93 (MQ=37)
                                                          cTGTCATTAACATGAGCACCGTGTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTccc                              <  1:1692416/93‑1 (MQ=37)
                                                           tGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTcccc                             >  2:3085465/1‑93 (MQ=37)
                                                           tGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTcccc                             >  2:3359920/1‑93 (MQ=37)
                                                           tGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTcccc                             <  2:3524666/93‑1 (MQ=37)
                                                            gTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCt                            >  1:4522276/1‑93 (MQ=37)
                                                                ttAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCTcc                        >  2:1369619/1‑93 (MQ=25)
                                                                ttAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCAcc                        >  2:4360257/1‑93 (MQ=25)
                                                                   aCATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTa                     <  1:4276925/93‑1 (MQ=21)
                                                                   aCATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTa                     >  1:4338666/1‑93 (MQ=21)
                                                                    cATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTaa                    <  1:3042541/93‑1 (MQ=21)
                                                                     aTGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAAc                   >  1:2320777/1‑93 (MQ=21)
                                                                         gCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTTCCCCCCCCCCCAccccc               >  2:4155983/1‑91 (MQ=18)
                                                                         gCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCctc               <  2:1498509/93‑1 (MQ=18)
                                                                         gCACCGTTTTC‑TCCCTCTCCCTGCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCctc               <  2:3958926/93‑1 (MQ=18)
                                                                          cACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCctct              <  1:2720669/93‑1 (MQ=18)
                                                                           aCCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCtctc             <  1:920949/93‑1 (MQ=18)
                                                                             cGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCcccc           >  1:178225/1‑93 (MQ=18)
                                                                              gTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTcccc          <  2:3605194/93‑1 (MQ=17)
                                                                                   tctccctctcccTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAc      <  2:4530669/89‑1 (MQ=18)
                                                                                   tctccctctcccTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAc      <  1:3487197/89‑1 (MQ=18)
                                                                                      ccctctcccTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACggg   <  1:3294758/92‑1 (MQ=18)
                                                                                      ccctctcccTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACggg   <  1:2586593/92‑1 (MQ=18)
                                                                                       cctctcccTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCCCCCCCACCCCCTCCCCAAAcacgg  >  2:1192535/1‑89 (MQ=18)
                                                                                       cctctcccTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACgggg  <  2:1699885/93‑1 (MQ=255)
                                                                                       cctctcccTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACgggg  <  1:2767414/93‑1 (MQ=255)
                                                                                            |                                                                                       
ATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑T‑CCTCT‑CCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGG  >  NC_000913/3237156‑3237332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑T‑CCTCT‑CCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGG  >  NC_000913/3237161‑3237339
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CGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCA                                                                                         >  SRR4302535.773394/1‑94 (MQ=60)
CGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCA                                                                                         >  SRR4302535.3276002/1‑94 (MQ=60)
CGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCA                                                                                         <  SRR4302535.101727/94‑1 (MQ=60)
CGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCATTTTC‑TCCCTCTCCCTTCCA                                                                                         >  SRR4302535.2763245/1‑94 (MQ=60)
  GCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGG                                                                                       <  SRR4302535.1929789/94‑1 (MQ=60)
  GCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGG                                                                                       <  SRR4302535.672727/94‑1 (MQ=60)
  GCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGG                                                                                       <  SRR4302535.3478279/94‑1 (MQ=60)
  GCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGG                                                                                       <  SRR4302535.4329102/94‑1 (MQ=60)
    ATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGA                                                                                     >  SRR4302535.1086275/1‑94 (MQ=60)
     TGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAG                                                                                    <  SRR4302535.2149809/94‑1 (MQ=60)
     TGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAG                                                                                    <  SRR4302535.3820608/94‑1 (MQ=60)
     TGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAG                                                                                    <  SRR4302535.4646043/94‑1 (MQ=60)
     TGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAG                                                                                    <  SRR4302535.4419562/94‑1 (MQ=60)
      GATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGG                                                                                   <  SRR4302535.118777/94‑1 (MQ=60)
      GATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGG                                                                                   <  SRR4302535.1824079/94‑1 (MQ=60)
       ATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGG                                                                                  <  SRR4302535.4437141/94‑1 (MQ=60)
       ATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGG                                                                                  <  SRR4302535.3377451/94‑1 (MQ=60)
       ATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGG                                                                                  <  SRR4302535.302604/94‑1 (MQ=60)
         AAGCAGCGGGGTGGTTAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCGCCGTTTTCAC‑CCTCTCCCTTCCAGGGAGGGGG                                                                                <  SRR4302535.4047002/94‑1 (MQ=60)
           GCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTGACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTC                                                                              >  SRR4302535.2705065/1‑94 (MQ=60)
             AGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGG                                                                            >  SRR4302535.3452265/1‑94 (MQ=60)
              GCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGG                                                                           >  SRR4302535.2010576/1‑94 (MQ=60)
              GCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGG                                                                           <  SRR4302535.2634646/94‑1 (MQ=60)
                GGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGT                                                                         <  SRR4302535.3984988/94‑1 (MQ=60)
                 GGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTG                                                                        <  SRR4302535.768168/94‑1 (MQ=60)
                  GGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGA                                                                       >  SRR4302535.3034447/1‑94 (MQ=60)
                   GTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAG                                                                      <  SRR4302535.4133158/94‑1 (MQ=60)
                    TGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGG                                                                     <  SRR4302535.871068/94‑1 (MQ=60)
                     GGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCATCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGG                                                                    <  SRR4302535.2530346/94‑1 (MQ=60)
                     GGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGG                                                                    >  SRR4302535.3735200/1‑94 (MQ=60)
                      GATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGT                                                                   <  SRR4302535.1080827/94‑1 (MQ=60)
                       ATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTA                                                                  >  SRR4302535.2084853/1‑94 (MQ=60)
                       ATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTA                                                                  >  SRR4302535.681884/1‑94 (MQ=60)
                       ATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTA                                                                  >  SRR4302535.4122185/1‑94 (MQ=60)
                       ATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTA                                                                  >  SRR4302535.3625676/1‑94 (MQ=60)
                       ATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGGGAGGGTA                                                                  >  SRR4302535.3315974/1‑94 (MQ=60)
                       ATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGAGAGGGTA                                                                  >  SRR4302535.2149346/1‑94 (MQ=60)
                          ATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATT                                                               >  SRR4302535.3360443/1‑94 (MQ=60)
                          ATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATT                                                               <  SRR4302535.304789/94‑1 (MQ=60)
                           TTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTT                                                              <  SRR4302535.729681/94‑1 (MQ=60)
                             TTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTT                                                            >  SRR4302535.253839/1‑94 (MQ=60)
                             TTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTT                                                            <  SRR4302535.4220380/94‑1 (MQ=60)
                               TAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCG                                                          <  SRR4302535.3442101/94‑1 (MQ=60)
                               TAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCG                                                          >  SRR4302535.3308976/1‑94 (MQ=60)
                               TAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCG                                                          <  SRR4302535.2341662/94‑1 (MQ=60)
                               TAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCG                                                          >  SRR4302535.1591225/1‑94 (MQ=60)
                                AATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGC                                                         >  SRR4302535.447372/1‑94 (MQ=60)
                                AATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGC                                                         >  SRR4302535.1495480/1‑94 (MQ=60)
                                   CTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACC                                                      <  SRR4302535.2865941/94‑1 (MQ=60)
                                   CTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACC                                                      >  SRR4302535.4343186/1‑94 (MQ=60)
                                   CTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACC                                                      >  SRR4302535.2620098/1‑94 (MQ=60)
                                     GCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGA                                                    <  SRR4302535.4177704/94‑1 (MQ=60)
                                       CTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATG                                                  <  SRR4302535.4009873/94‑1 (MQ=60)
                                       CTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATG                                                  >  SRR4302535.2051868/1‑94 (MQ=60)
                                       CTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATG                                                  >  SRR4302535.2609645/1‑94 (MQ=60)
                                       CTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATG                                                  >  SRR4302535.3173609/1‑94 (MQ=60)
                                       CTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATG                                                  >  SRR4302535.770064/1‑94 (MQ=60)
                                       CTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATG                                                  <  SRR4302535.1266926/94‑1 (MQ=60)
                                             CGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCC                                            >  SRR4302535.792785/1‑94 (MQ=60)
                                              GTGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCT                                           >  SRR4302535.3873244/1‑94 (MQ=60)
                                               TGTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTG                                          <  SRR4302535.273953/94‑1 (MQ=60)
                                                GTACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGT                                         <  SRR4302535.2517613/94‑1 (MQ=60)
                                                 TACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTT                                        >  SRR4302535.3227614/1‑94 (MQ=60)
                                                 TACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTT                                        >  SRR4302535.4364391/1‑94 (MQ=60)
                                                 TACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTT                                        >  SRR4302535.3371424/1‑94 (MQ=60)
                                                 TACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTT                                        >  SRR4302535.4491693/1‑94 (MQ=60)
                                                 TACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTT                                        >  SRR4302535.1626454/1‑94 (MQ=60)
                                                  ACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTC                                       >  SRR4302535.2669777/1‑94 (MQ=60)
                                                  ACCCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTC                                       <  SRR4302535.4635408/94‑1 (MQ=60)
                                                    CCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTGCC                                     >  SRR4302535.3729126/1‑94 (MQ=60)
                                                    CCTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCC                                     >  SRR4302535.213557/1‑94 (MQ=60)
                                                     CTGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGCTACCC                                    >  SRR4302535.858512/1‑94 (MQ=60)
                                                     CTGTCATTAACATGAGCACCGTGTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCC                                    <  SRR4302535.1704657/94‑1 (MQ=60)
                                                      TGTCATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCT                                   >  SRR4302535.4553265/1‑94 (MQ=60)
                                                          ATTAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTtcccccccc                               >  SRR4302535.376830/1‑85 (MQ=60)
                                                            TAACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTTCCCCCACCCC                             >  SRR4302535.155905/1‑94 (MQ=60)
                                                             AACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTA                            >  SRR4302535.4368335/1‑94 (MQ=60)
                                                              ACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCcccccccca                           >  SRR4302535.980190/1‑85 (MQ=60)
                                                              ACATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAA                           <  SRR4302535.4306198/94‑1 (MQ=60)
                                                               CATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAAC                          <  SRR4302535.3063860/94‑1 (MQ=60)
                                                               CATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAAC                          >  SRR4302535.2337388/1‑94 (MQ=60)
                                                                ATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTttccccacccccccc                         >  SRR4302535.1654088/1‑79 (MQ=60)
                                                                ATGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACC                         >  SRR4302535.528836/1‑94 (MQ=60)
                                                                 TGAGCACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCC                        >  SRR4302535.3864664/1‑94 (MQ=60)
                                                                     CACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTC                    <  SRR4302535.2740104/94‑1 (MQ=60)
                                                                      ACCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCC                   <  SRR4302535.927481/94‑1 (MQ=60)
                                                                       CCGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCCCCC                  >  SRR4302535.179463/1‑94 (MQ=60)
                                                                        CGTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCC                 >  SRR4302535.2295063/1‑94 (MQ=60)
                                                                         GTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCA                <  SRR4302535.630633/94‑1 (MQ=60)
                                                                         GTTTTC‑TCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCA                >  SRR4302535.3157061/1‑94 (MQ=60)
                                                                             ttctccctctcCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAC             <  SRR4302535.173817/83‑1 (MQ=60)
                                                                              tctctctctcCCTTCCAGGGAGGTGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACG            <  SRR4302535.4554339/84‑1 (MQ=60)
                                                                              tctccctctcCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACG            <  SRR4302535.1667527/84‑1 (MQ=60)
                                                                              tctccctctcCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACG            <  SRR4302535.3511407/84‑1 (MQ=60)
                                                                               ctccctctcCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGG           <  SRR4302535.3692013/85‑1 (MQ=60)
                                                                                 ccctctcCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTtttctccccccccccccccccccccccccac         >  SRR4302535.823785/8‑63 (MQ=60)
                                                                                 ccctctcCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGG         <  SRR4302535.2605170/87‑1 (MQ=60)
                                                                                 ccctctcCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGG         <  SRR4302535.3317661/87‑1 (MQ=60)
                                                                                 ccctctcCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGG         <  SRR4302535.1737287/87‑1 (MQ=60)
                                                                                  CCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGC        <  SRR4302535.2787119/94‑1 (MQ=60)
                                                                                  CCTC‑‑CCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGA      <  SRR4302535.1976389/94‑1 (MQ=60)
                                                                                   CTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCG       <  SRR4302535.3814353/94‑1 (MQ=60)
                                                                                   CTC‑‑CCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCTCCTCACCCcctacccctcccccaacgcggcgag     >  SRR4302535.295885/1‑69 (MQ=60)
                                                                                   CTC‑‑CCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAG     <  SRR4302535.1924198/94‑1 (MQ=60)
                                                                                   CTC‑‑CCTTCCAGGGAGGGGGTCGGGGGGAGGGTAATTTTTCGCACCGATGCTGGCCTGgttccccccccctcaaccccccccccccccgggcggg     >  SRR4302535.3466140/1‑57 (MQ=60)
                                                                                      tcCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGG    <  SRR4302535.1332454/92‑1 (MQ=60)
                                                                                      tcCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGG    <  SRR4302535.3221909/92‑1 (MQ=60)
                                                                                      tcCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGG    <  SRR4302535.381894/92‑1 (MQ=60)
                                                                                      tcCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCCCTCCCCAAAAGGGGCGAGG    >  SRR4302535.1374680/3‑94 (MQ=60)
                                                                                      C‑CCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTtccccccccccccccccccccccccaccaggcgcggg   >  SRR4302535.116243/1‑57 (MQ=60)
                                                                                      C‑CCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCCCCCTAACCCTCTCCCCAAACGGGGCGAGGG   <  SRR4302535.3239023/94‑1 (MQ=60)
                                                                                      C‑CCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGG   <  SRR4302535.4201997/94‑1 (MQ=60)
                                                                                        CCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGG  <  SRR4302535.4256302/94‑1 (MQ=60)
                                                                                         |                                                                                            
CGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTC‑T‑CCTCT‑CCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGG  >  NC_000913/3237161‑3237339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑