Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,257,838	+G	coding (2222/2241 nt)	tdcE ←	putative formate acetyltransferase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,257,837	1	.	G	99.1%	717.4 / ‑1.5	229	coding (2223/2241 nt)	tdcE	putative formate acetyltransferase 3
Reads supporting (aligned to +/- strand): ref base . (2/0); new base G (102/125); total (104/125)
Fisher's exact test for biased strand distribution p-value = 2.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

Only 100 of 251 total aligned reads displayed.

TTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAC‑G‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTT  >  NC_000913/3257745‑3257923

tttttCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAc                                                                                          <  1:1448169/93‑1 (MQ=255)

tttttCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAc                                                                                          <  1:3724022/93‑1 (MQ=255)

 ttttCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAc‑g                                                                                        >  1:3160593/1‑93 (MQ=255)

       tACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAg                                                                                  >  1:458966/1‑93 (MQ=255)

       tACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAg                                                                                  <  2:3669950/93‑1 (MQ=255)

          aTCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCa                                                                               >  1:3320951/1‑93 (MQ=255)

          aTCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCa                                                                               <  2:2895503/93‑1 (MQ=255)

           tcctccGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCag                                                                              <  2:3314820/93‑1 (MQ=255)

           tcctccGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCag                                                                              >  1:2241634/1‑93 (MQ=255)

           tcctccGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCag                                                                              >  1:855071/1‑93 (MQ=255)

           tcctccGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCag                                                                              >  1:2631395/1‑93 (MQ=255)

            cctccGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCaga                                                                             >  2:3559221/1‑93 (MQ=255)

            cctccGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCaga                                                                             <  2:3519816/93‑1 (MQ=255)

             ctccGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCagag                                                                            <  2:12655/93‑1 (MQ=255)

             ctccGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCagag                                                                            <  1:3500650/93‑1 (MQ=255)

              cccGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAgaga                                                                           <  1:1631429/92‑1 (MQ=255)

                cGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGaca                                                                         <  2:4287869/93‑1 (MQ=255)

                cGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGaca                                                                         <  1:4155788/93‑1 (MQ=255)

                cGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGaca                                                                         <  1:3355840/93‑1 (MQ=255)

                     tCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGAt                                                                    <  2:1054953/93‑1 (MQ=255)

                      cAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGAtt                                                                   <  1:2368750/93‑1 (MQ=255)

                      cAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGAtt                                                                   <  2:4176988/93‑1 (MQ=255)

                      cAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGAtt                                                                   >  1:3591581/1‑93 (MQ=255)

                      cAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGAtt                                                                   <  2:201918/93‑1 (MQ=255)

                       agagCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTg                                                                  >  2:318647/1‑93 (MQ=255)

                        gagCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGt                                                                 <  2:1137844/93‑1 (MQ=255)

                        gagCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGt                                                                 <  2:2877291/93‑1 (MQ=255)

                         ggcgcCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTc                                                                <  1:2129480/92‑1 (MQ=255)

                         agCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTc                                                                <  1:3763305/93‑1 (MQ=255)

                          gcgcCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCa                                                               <  2:635730/93‑1 (MQ=255)

                          gcgcCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCa                                                               <  1:4254949/93‑1 (MQ=255)

                          gcgcCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGAACGG‑CGTAGCCAGAGACACGGACTGTCa                                                               >  1:337871/1‑93 (MQ=255)

                              cTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGtt                                                           >  2:2122298/1‑93 (MQ=255)

                              cTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGtt                                                           >  1:4222814/1‑93 (MQ=255)

                               tGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTa                                                          >  1:4463215/1‑93 (MQ=255)

                               tGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTa                                                          <  2:831133/93‑1 (MQ=255)

                                gggTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAg                                                         >  1:3103715/1‑93 (MQ=255)

                                gggTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAg                                                         <  1:396727/93‑1 (MQ=255)

                                 ggTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAgg                                                        >  2:1727360/1‑93 (MQ=255)

                                 ggTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAgg                                                        >  2:739087/1‑93 (MQ=255)

                                   tAAAGGTGCGTGAAATAACATCCTGTTGCTGTGCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGat                                                      <  1:434961/93‑1 (MQ=255)

                                   tAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGat                                                      >  1:1883803/1‑93 (MQ=255)

                                    aaaGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGata                                                     <  2:1548251/93‑1 (MQ=255)

                                    aaaGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGata                                                     <  1:509624/93‑1 (MQ=255)

                                    aaaGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGata                                                     <  2:3104557/93‑1 (MQ=255)

                                     aaGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGatat                                                    >  1:1227002/1‑93 (MQ=255)

                                      aGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTCGCCAGAGACACGGATTGTCAGGTTAGGATAtt                                                   >  1:741597/1‑93 (MQ=255)

                                      aGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATAtt                                                   >  2:3925364/1‑93 (MQ=255)

                                      aGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATAtt                                                   <  2:724000/93‑1 (MQ=255)

                                         tACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATAttttt                                                >  1:3954555/1‑93 (MQ=255)

                                         tACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATAttttt                                                <  1:2741409/93‑1 (MQ=255)

                                         tACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATAttttt                                                >  1:4549744/1‑93 (MQ=255)

                                           cGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTcc                                              >  2:3878105/1‑93 (MQ=255)

                                           cGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTcc                                              <  1:3310715/93‑1 (MQ=255)

                                            gTGAAATACCATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑AGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCg                                             >  2:4553037/1‑93 (MQ=255)

                                            gTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCg                                             <  1:1014136/93‑1 (MQ=255)

                                              gAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAC‑GCCGTAGCCAGAGACACGGATTGTCAGGGTAGGATATTTTTCCggg                                           >  2:3641491/1‑93 (MQ=255)

                                                aaTAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTg                                         <  2:4629350/93‑1 (MQ=255)

                                                aaTAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTg                                         >  1:1053840/1‑93 (MQ=255)

                                                 aTAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGc                                        <  2:4249386/93‑1 (MQ=255)

                                                  tAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCt                                       >  2:1331713/1‑93 (MQ=255)

                                                   aaCATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTc                                      <  1:2864048/93‑1 (MQ=255)

                                                   aaCATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTc                                      <  2:4483942/93‑1 (MQ=255)

                                                   aaCATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTc                                      >  1:573523/1‑93 (MQ=255)

                                                    aCATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑GGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTGCGGGTGCTCg                                     >  2:1689001/1‑93 (MQ=255)

                                                    aCATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCg                                     >  2:2706841/1‑93 (MQ=255)

                                                     cATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGa                                    <  1:1198986/93‑1 (MQ=255)

                                                     cATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGa                                    <  1:1385856/93‑1 (MQ=255)

                                                     cATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGa                                    <  1:216664/93‑1 (MQ=255)

                                                       tCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATg                                  <  1:2669323/93‑1 (MQ=255)

                                                       tCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATg                                  >  1:2755442/1‑93 (MQ=255)

                                                       tCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATg                                  >  1:2584814/1‑93 (MQ=255)

                                                       tCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATg                                  >  2:231218/1‑93 (MQ=255)

                                                         cTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGc                                >  2:1196932/1‑93 (MQ=255)

                                                            ttGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATc                             >  1:2486768/1‑93 (MQ=255)

                                                            gtGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATc                             <  1:3110165/92‑1 (MQ=255)

                                                             tGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCAtac                            >  2:1446529/1‑91 (MQ=255)

                                                              gCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCca                           >  2:2958892/1‑93 (MQ=255)

                                                              gCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCca                           <  2:2523969/93‑1 (MQ=255)

                                                               cTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCcag                          >  1:682755/1‑93 (MQ=255)

                                                                tGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCcagc                         >  1:4071376/1‑93 (MQ=255)

                                                                   tCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCagcagc                      <  1:3197832/93‑1 (MQ=255)

                                                                     ccGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCAt                    <  2:942551/92‑1 (MQ=255)

                                                                     aCGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCAt                    <  2:701678/93‑1 (MQ=255)

                                                                      cGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCAtt                   <  2:516903/93‑1 (MQ=255)

                                                                        ggTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTc                 >  2:2767927/1‑93 (MQ=255)

                                                                        ggTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTc                 <  2:2355484/93‑1 (MQ=255)

                                                                         gTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTcc                <  1:4128689/93‑1 (MQ=255)

                                                                          tCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTccc               <  2:434151/93‑1 (MQ=255)

                                                                          tCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTccc               <  2:2939615/93‑1 (MQ=255)

                                                                            aGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGa             >  1:4012000/1‑93 (MQ=255)

                                                                             gTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGAt            >  2:3259565/1‑93 (MQ=255)

                                                                                cGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCa         >  1:4647637/1‑93 (MQ=255)

                                                                                 gTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCAt        >  2:1973086/1‑93 (MQ=255)

                                                                                 gTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCAt        <  2:1770098/93‑1 (MQ=255)

                                                                                  ttGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCAtt       >  1:4233788/1‑93 (MQ=255)

                                                                                  ttGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCAtt       <  2:2583822/93‑1 (MQ=255)

                                                                                  ttGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCAtt       <  1:202580/93‑1 (MQ=255)

                                                                                   tGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTa      <  2:2228515/93‑1 (MQ=255)

                                                                                    gAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGTTTCATTac     <  2:4611779/93‑1 (MQ=255)

TTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAC‑G‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTT  >  NC_000913/3257745‑3257923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑C‑G‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTG  >  NC_000913/3257745‑3257930

TTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑C‑G                                                                                               >  SRR4302535.3182520/1‑94 (MQ=60)

TTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑C‑G                                                                                               <  SRR4302535.3749820/94‑1 (MQ=60)

TTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑C‑G                                                                                               <  SRR4302535.3780522/94‑1 (MQ=60)

TTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑C‑G                                                                                               <  SRR4302535.4030838/94‑1 (MQ=60)

TTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑C‑G                                                                                               <  SRR4302535.1458523/94‑1 (MQ=60)

     CATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTA                                                                                          >  SRR4302535.3128617/1‑94 (MQ=60)

     CATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTA                                                                                          <  SRR4302535.2137221/94‑1 (MQ=60)

      ATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAG                                                                                         >  SRR4302535.4701457/1‑94 (MQ=60)

      ATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAG                                                                                         >  SRR4302535.462176/1‑94 (MQ=60)

      ATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAG                                                                                         >  SRR4302535.462177/1‑94 (MQ=60)

         CATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCA                                                                                      >  SRR4302535.3344060/1‑94 (MQ=60)

          ATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAG                                                                                     >  SRR4302535.861098/1‑94 (MQ=60)

          ATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAG                                                                                     >  SRR4302535.3130987/1‑94 (MQ=60)

          ATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAG                                                                                     >  SRR4302535.2650246/1‑94 (MQ=60)

          ATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAG                                                                                     >  SRR4302535.2257582/1‑94 (MQ=60)

             CTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGA                                                                                  >  SRR4302535.2965289/1‑94 (MQ=60)

             CTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGA                                                                                  <  SRR4302535.3524988/94‑1 (MQ=60)

              TCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGAC                                                                                 <  SRR4302535.2571514/94‑1 (MQ=60)

              TCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGAC                                                                                 <  SRR4302535.247551/94‑1 (MQ=60)

              CCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGAC                                                                                 <  SRR4302535.1643215/94‑1 (MQ=60)

                CGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACAC                                                                               >  SRR4302535.4588466/1‑94 (MQ=60)

                CGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACAC                                                                               <  SRR4302535.3379207/94‑1 (MQ=60)

                CGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACAC                                                                               <  SRR4302535.4027731/94‑1 (MQ=60)

                CGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACAC                                                                               <  SRR4302535.4184237/94‑1 (MQ=60)

                 GGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACG                                                                              >  SRR4302535.3351142/1‑94 (MQ=60)

                 GGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACG                                                                              <  SRR4302535.3388789/94‑1 (MQ=60)

                 GGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACG                                                                              <  SRR4302535.1455626/94‑1 (MQ=60)

                 GGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑C‑GCCGTAGCCAGAGACACG                                                                              >  SRR4302535.32879/1‑94 (MQ=60)

                   CGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGA                                                                            <  SRR4302535.2668254/94‑1 (MQ=60)

                    GTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGAT                                                                           >  SRR4302535.520613/1‑94 (MQ=60)

                     TCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATT                                                                          <  SRR4302535.4085889/94‑1 (MQ=60)

                     TCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATT                                                                          <  SRR4302535.119563/94‑1 (MQ=60)

                     TCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATT                                                                          <  SRR4302535.3986858/94‑1 (MQ=60)

                     TCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATT                                                                          >  SRR4302535.3616493/1‑94 (MQ=60)

                     TCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATT                                                                          <  SRR4302535.1209365/94‑1 (MQ=60)

                      CAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTG                                                                         <  SRR4302535.2385756/94‑1 (MQ=60)

                        GAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTC                                                                       <  SRR4302535.2334733/94‑1 (MQ=60)

                         GGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCA                                                                      <  SRR4302535.2144831/94‑1 (MQ=60)

                         AGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCA                                                                      >  SRR4302535.3467027/1‑94 (MQ=60)

                         AGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCA                                                                      <  SRR4302535.3789303/94‑1 (MQ=60)

                         AGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGAA‑CGG‑CGTAGCCAGAGACACGGACTGTCA                                                                      >  SRR4302535.340209/1‑94 (MQ=60)

                          GCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAG                                                                     <  SRR4302535.651024/94‑1 (MQ=60)

                          GCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAG                                                                     <  SRR4302535.4284098/94‑1 (MQ=60)

                           CGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGG                                                                    <  SRR4302535.4596845/94‑1 (MQ=60)

                             CCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTT                                                                  >  SRR4302535.4251706/1‑94 (MQ=60)

                              CTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTA                                                                 >  SRR4302535.4219644/1‑94 (MQ=60)

                              CTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTA                                                                 >  SRR4302535.4493829/1‑94 (MQ=60)

                               TGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAG                                                                >  SRR4302535.3125322/1‑94 (MQ=60)

                                GGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGG                                                               <  SRR4302535.399505/94‑1 (MQ=60)

                                  GTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGAT                                                             <  SRR4302535.76796/94‑1 (MQ=60)

                                  GTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGAT                                                             >  SRR4302535.1897381/1‑94 (MQ=60)

                                  GTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGAT                                                             <  SRR4302535.3152859/94‑1 (MQ=60)

                                   TAAAGGTGCGTGAAATAACATCCTGTTGCTGTGCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATA                                                            <  SRR4302535.437991/94‑1 (MQ=60)

                                    AAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGACGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATAT                                                           <  SRR4302535.2535027/94‑1 (MQ=60)

                                    AAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATAT                                                           <  SRR4302535.513187/94‑1 (MQ=60)

                                    AAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATAT                                                           >  SRR4302535.1235776/1‑94 (MQ=60)

                                    AAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATAT                                                           <  SRR4302535.3210376/94‑1 (MQ=60)

                                     AAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTCGCCAGAGACACGGATTGTCAGGTTAGGATATT                                                          >  SRR4302535.746807/1‑94 (MQ=60)

                                     AAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATT                                                          <  SRR4302535.3653552/94‑1 (MQ=60)

                                      AGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTT                                                         >  SRR4302535.781049/1‑94 (MQ=60)

                                      AGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTT                                                         >  SRR4302535.3298131/1‑94 (MQ=60)

                                        GTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTT                                                       >  SRR4302535.3165115/1‑94 (MQ=60)

                                        GTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTT                                                       <  SRR4302535.2686979/94‑1 (MQ=60)

                                        GTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTT                                                       >  SRR4302535.4580846/1‑94 (MQ=60)

                                        GTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTT                                                       >  SRR4302535.3981551/1‑94 (MQ=60)

                                        GTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGGTAGGATATTTTT                                                       >  SRR4302535.2765882/1‑94 (MQ=60)

                                         TACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTC                                                      <  SRR4302535.2760947/94‑1 (MQ=60)

                                           CGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCG                                                    <  SRR4302535.3333752/94‑1 (MQ=60)

                                            GTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGG                                                   >  SRR4302535.1040795/1‑94 (MQ=60)

                                            GTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGG                                                   <  SRR4302535.1021345/94‑1 (MQ=60)

                                             TGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGG                                                  >  SRR4302535.721833/1‑94 (MQ=60)

                                               AAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTG                                                >  SRR4302535.1061333/1‑94 (MQ=60)

                                               AAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTG                                                >  SRR4302535.2764700/1‑94 (MQ=60)

                                               AAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTG                                                >  SRR4302535.4280275/1‑94 (MQ=60)

                                               AAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTG                                                >  SRR4302535.2633220/1‑94 (MQ=60)

                                               AAAGAACATCCGGGTGCTGTGTACGGGTCAGTGCGTTGAAGCGCATG‑G‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTG                                                <  SRR4302535.3683902/94‑1 (MQ=60)

                                                  TAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTC                                             >  SRR4302535.577548/1‑94 (MQ=60)

                                                  TAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTC                                             >  SRR4302535.1336014/1‑94 (MQ=60)

                                                  TAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTC                                             >  SRR4302535.1897524/1‑94 (MQ=60)

                                                   AACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCG                                            <  SRR4302535.2884340/94‑1 (MQ=60)

                                                   AACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCG                                            <  SRR4302535.2169989/94‑1 (MQ=60)

                                                   AACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCG                                            <  SRR4302535.2169990/94‑1 (MQ=60)

                                                     CATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGAT                                          <  SRR4302535.218156/94‑1 (MQ=60)

                                                     CATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGAT                                          <  SRR4302535.1207576/94‑1 (MQ=60)

                                                     CATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGAT                                          <  SRR4302535.1395745/94‑1 (MQ=60)

                                                      ATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATG                                         >  SRR4302535.3838511/1‑94 (MQ=60)

                                                      ATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATG                                         <  SRR4302535.3866387/94‑1 (MQ=60)

                                                      ATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATG                                         >  SRR4302535.2603379/1‑94 (MQ=60)

                                                      ATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATG                                         <  SRR4302535.1563613/94‑1 (MQ=60)

                                                      ATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATG                                         >  SRR4302535.1854386/1‑94 (MQ=60)

                                                      ATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATG                                         >  SRR4302535.2775082/1‑94 (MQ=60)

                                                       TCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGG                                        <  SRR4302535.2688425/94‑1 (MQ=60)

                                                         CTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCA                                      >  SRR4302535.4109707/1‑94 (MQ=60)

                                                          TGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTTCGAGTGCTCGATGGCAT                                     >  SRR4302535.3334360/1‑94 (MQ=60)

                                                          TGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCAT                                     >  SRR4302535.1321289/1‑94 (MQ=60)

                                                           GTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATC                                    >  SRR4302535.2504699/1‑94 (MQ=60)

                                                            TTGCTGTGCACGTGTCAGTGCGGTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCC                                   <  SRR4302535.4444277/94‑1 (MQ=60)

                                                            GTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCC                                   <  SRR4302535.3131818/94‑1 (MQ=60)

                                                             TGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCA                                  <  SRR4302535.4180380/94‑1 (MQ=60)

                                                             TGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCA                                  >  SRR4302535.4434867/1‑94 (MQ=60)

                                                              GCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAG                                 >  SRR4302535.687535/1‑94 (MQ=60)

                                                              GCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAG                                 >  SRR4302535.356629/1‑94 (MQ=60)

                                                               CTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGC                                >  SRR4302535.4099237/1‑94 (MQ=60)

                                                                TGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCA                               >  SRR4302535.296743/1‑94 (MQ=60)

                                                                 GTTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAG                              <  SRR4302535.4134625/94‑1 (MQ=60)

                                                                  TTCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGC                             >  SRR4302535.4682626/1‑94 (MQ=60)

                                                                   TCACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCA                            <  SRR4302535.3219960/94‑1 (MQ=60)

                                                                    CACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCAT                           >  SRR4302535.668734/1‑94 (MQ=60)

                                                                    CACGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCAT                           <  SRR4302535.4192854/94‑1 (MQ=60)

                                                                      CGGGTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTT                         <  SRR4302535.2260443/94‑1 (MQ=60)

                                                                         GTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCC                      <  SRR4302535.4156942/94‑1 (MQ=60)

                                                                         GTCAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGAAGCATTTCCC                      >  SRR4302535.3360910/1‑94 (MQ=60)

                                                                           CAGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGA                    >  SRR4302535.4039447/1‑94 (MQ=60)

                                                                            AGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGAT                   <  SRR4302535.4252235/94‑1 (MQ=60)

                                                                            AGTGCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGAT                   <  SRR4302535.2185985/94‑1 (MQ=60)

                                                                               GCGTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCA                >  SRR4302535.4679119/1‑94 (MQ=60)

                                                                                 GTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATT              >  SRR4302535.4262760/1‑94 (MQ=60)

                                                                                 GTTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATT              <  SRR4302535.1923700/94‑1 (MQ=60)

                                                                                  TTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTA             <  SRR4302535.203961/94‑1 (MQ=60)

                                                                                  TTGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTA             >  SRR4302535.1558141/1‑94 (MQ=60)

                                                                                   TGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTAC            <  SRR4302535.1105827/94‑1 (MQ=60)

                                                                                   TGAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTAC            >  SRR4302535.2754465/1‑94 (MQ=60)

                                                                                    GAAGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACG           <  SRR4302535.4515467/94‑1 (MQ=60)

                                                                                      AGCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTT         <  SRR4302535.4277429/94‑1 (MQ=60)

                                                                                       GCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTG        <  SRR4302535.2873999/94‑1 (MQ=60)

                                                                                       GCGCA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTG        <  SRR4302535.3513894/94‑1 (MQ=60)

                                                                                          CA‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACG     >  SRR4302535.1615925/1‑94 (MQ=60)

                                                                                             cgG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTT   >  SRR4302535.363986/3‑94 (MQ=60)

                                                                                             G‑G‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTG  <  SRR4302535.1424586/94‑1 (MQ=60)

                                                                                             G‑G‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTG  <  SRR4302535.2510588/94‑1 (MQ=60)

                                                                                             G‑G‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTG  <  SRR4302535.4300424/94‑1 (MQ=60)

                                                                                             G‑G‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTACCGATTCATTACGTTGACGTTG  >  SRR4302535.261765/1‑94 (MQ=60)

TTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑C‑G‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTG  >  NC_000913/3257745‑3257930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`