Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,257,952 T→C D703G (GAT→GGT)  tdcE ← putative formate acetyltransferase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,257,9520TC99.1% 1002.4 / ‑2.0 325D703G (GAT→GGT) tdcEputative formate acetyltransferase 3
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base C (136/184);  total (139/186)
Fisher's exact test for biased strand distribution p-value = 5.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

Only 100 of 339 total aligned reads displayed.

AGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTG  >  NC_000913/3257862‑3258042
                                                                                          |                                                                                          
aGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACAccc                                                                                          <  2:3848929/93‑1 (MQ=255)
 ggTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCg                                                                                         <  1:6622017/93‑1 (MQ=255)
  gTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGc                                                                                        <  2:1544958/93‑1 (MQ=255)
   ttAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCt                                                                                       <  2:1341596/93‑1 (MQ=255)
   ttAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCt                                                                                       >  2:6706353/1‑93 (MQ=255)
   ttAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCt                                                                                       <  1:5533916/93‑1 (MQ=255)
   ttAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCt                                                                                       >  2:7512637/1‑93 (MQ=255)
      ggATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCg                                                                                    >  1:5057201/1‑93 (MQ=255)
         tatTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCgtgg                                                                                 >  2:7390602/1‑93 (MQ=255)
         tatTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCgtgg                                                                                 >  2:3410492/1‑93 (MQ=255)
               tCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGaaa                                                                           <  1:6151929/93‑1 (MQ=255)
                tcGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAAt                                                                          <  1:2603440/92‑1 (MQ=255)
                ccGGGTTCTCGATGGCATCCAGCAGCATTTCCCAATTCATTACGTTGACGTTGAGGTGGTGACCGCCTTCGACACCCGCTTCGTGGTGGAAAt                                                                          <  2:7138190/93‑1 (MQ=255)
                 cGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATa                                                                         <  1:253143/93‑1 (MQ=255)
                  gggTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATAc                                                                        >  2:568154/1‑93 (MQ=255)
                  gggTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATAc                                                                        <  2:4158918/93‑1 (MQ=255)
                      gCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCAt                                                                    <  2:1040372/93‑1 (MQ=255)
                         cGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCca                                                                 >  2:4369512/1‑93 (MQ=255)
                         cGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCca                                                                 <  1:2990922/93‑1 (MQ=255)
                         cGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCca                                                                 >  1:3262010/1‑93 (MQ=255)
                          gATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCcag                                                                >  2:5426640/1‑93 (MQ=255)
                          gATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCcac                                                                >  1:310572/1‑92 (MQ=255)
                           aTGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCcagc                                                               >  2:5444681/1‑93 (MQ=255)
                            tGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCcagca                                                              <  2:1198118/93‑1 (MQ=255)
                            tGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCcagca                                                              <  2:5915438/93‑1 (MQ=255)
                             ggCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCcagcag                                                             >  2:2970667/1‑93 (MQ=255)
                                aTCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGcc                                                          >  1:1710594/1‑93 (MQ=255)
                                 tCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCg                                                         >  2:6143360/1‑93 (MQ=255)
                                  ccAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGa                                                        <  1:838222/93‑1 (MQ=255)
                                   cagcagCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACGCCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGAc                                                       <  2:4847354/93‑1 (MQ=255)
                                   cagcagCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGAc                                                       >  1:6296138/1‑93 (MQ=255)
                                     gcagcaTTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACaa                                                     >  2:4572163/1‑93 (MQ=255)
                                      cagcaTTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAg                                                    >  2:6347441/1‑93 (MQ=255)
                                       agcaTTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAgg                                                   <  1:4503121/93‑1 (MQ=255)
                                        acaTTTCCCGATTCAGTACGTTGACGGTGGGGTGGTGACCGCCTTCGGCACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAggt                                                  <  1:1825916/92‑1 (MQ=255)
                                          aTTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAggttg                                                >  1:7330806/1‑93 (MQ=255)
                                          aTTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAggttg                                                <  2:2395323/93‑1 (MQ=255)
                                          aTTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAggttg                                                <  1:7224283/93‑1 (MQ=255)
                                          aTTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAggttg                                                >  1:3915098/1‑93 (MQ=255)
                                          aTTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAggttg                                                >  1:3852000/1‑93 (MQ=255)
                                          aTTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAggttg                                                >  1:3635158/1‑93 (MQ=255)
                                           tttCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAggttgg                                               >  1:1359208/1‑93 (MQ=255)
                                            ttCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAggttggt                                              >  1:1196519/1‑93 (MQ=255)
                                            ttCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAggttggt                                              <  1:1479532/93‑1 (MQ=255)
                                            ttCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAggttggt                                              <  1:2970843/93‑1 (MQ=255)
                                             tCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAggttggtt                                             <  1:1144148/93‑1 (MQ=255)
                                             tCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAggttggtt                                             <  1:4632658/93‑1 (MQ=255)
                                              cccGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGttt                                            <  1:3162741/93‑1 (MQ=255)
                                                cGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTa                                          <  2:4999389/93‑1 (MQ=255)
                                                 gATTCATTACGTTGACGTTGGGGCGTTCACCGCCTTCGACACCCGCTTCGTGCTGGAAATACCTATCCAGCAGACCGACAAGGTGGGTGTTAc                                         >  2:5495484/1‑93 (MQ=255)
                                                 gATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTAc                                         <  2:3139939/93‑1 (MQ=255)
                                                 gATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTAc                                         <  1:3379775/93‑1 (MQ=255)
                                                   ttCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCGGGCCGACAAGGTTGGTTTTaggc                                       >  2:5349547/1‑90 (MQ=255)
                                                   ttCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGt                                       <  1:537562/93‑1 (MQ=255)
                                                   ttCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGt                                       >  2:3459455/1‑93 (MQ=255)
                                                   ttCATTACGTTGACGTTGAGGTGTTGACCGCCATCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGt                                       >  1:7499263/1‑93 (MQ=255)
                                                    tCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTa                                      >  2:4804760/1‑93 (MQ=255)
                                                       ttACGTTGACGTTGAGGTGTTGACCGCCTTCGACTCCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTg                                   <  2:6572883/93‑1 (MQ=255)
                                                       ttACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTTCTg                                   <  2:4280606/93‑1 (MQ=255)
                                                       ttACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTg                                   <  1:4857390/93‑1 (MQ=255)
                                                       ttACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTg                                   <  2:3677244/93‑1 (MQ=255)
                                                       ttACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTg                                   <  1:1578960/93‑1 (MQ=255)
                                                       ttACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTg                                   >  2:2832544/1‑93 (MQ=255)
                                                        tACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTgg                                  >  2:211379/1‑93 (MQ=255)
                                                          cgttgacgttgtGGTGTTGACCGCCTGGTACAACCGCTTCGTGGTGGAAATACCCATGCAGCAGGCCGGCAAGGGTGTTTTTACGTACTCGAt                                >  2:7827185/1‑93 (MQ=255)
                                                            ttgacgttgaGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGAtct                              >  2:6974774/1‑93 (MQ=255)
                                                            ttgacgttgaGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGAtct                              >  1:2111866/1‑93 (MQ=255)
                                                            ttgacgttgaGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGAtct                              <  2:4724934/93‑1 (MQ=255)
                                                             tgacgttgaGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGAtctt                             >  1:2662064/1‑93 (MQ=255)
                                                             tgacgttgaGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGAtctt                             <  1:7172301/93‑1 (MQ=255)
                                                                cgttgaGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATcttctt                          <  2:5131478/93‑1 (MQ=255)
                                                                cgttgaGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATcttctt                          >  2:792740/1‑93 (MQ=255)
                                                                cgttgaGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATcttctt                          <  1:144884/93‑1 (MQ=255)
                                                                cgttgaGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATcttctt                          <  1:6545802/93‑1 (MQ=255)
                                                                 gttgaGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCttt                         >  2:4491569/1‑93 (MQ=255)
                                                                 gttgaGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCttt                         <  2:773691/93‑1 (MQ=255)
                                                                 gttgaGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCttt                         >  1:6031410/1‑93 (MQ=255)
                                                                  ttgaGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTa                                      >  2:2299041/1‑79 (MQ=255)
                                                                  ttgaGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTa                                      <  1:2299042/79‑1 (MQ=255)
                                                                   tgaGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACACATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGCTCTTCTTTGc                       >  2:2590229/1‑93 (MQ=255)
                                                                    gaGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGcc                      >  1:3594698/1‑93 (MQ=255)
                                                                     aGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGccc                     <  1:781823/93‑1 (MQ=255)
                                                                      ggTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCa                    <  2:1756175/93‑1 (MQ=255)
                                                                       gtgtTGACCGCCTTCTACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAg                   <  1:899038/93‑1 (MQ=255)
                                                                         gtTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAgcg                 >  1:1274195/1‑93 (MQ=255)
                                                                          ttGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAgcgc                >  1:1841945/1‑93 (MQ=255)
                                                                           tGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGCCAAGGTTGGTTTTACGTACTGGATCTTTTTTGCTCAGcgcc               >  1:2687843/1‑93 (MQ=38)
                                                                           tGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGcgcc               <  1:7742534/93‑1 (MQ=255)
                                                                            gACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGcgccg              <  1:1958132/93‑1 (MQ=255)
                                                                                gCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAgg          >  1:6688070/1‑93 (MQ=255)
                                                                                 ccTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGa         >  1:6792110/1‑93 (MQ=255)
                                                                                  cTTCGGCACCCCCTTCGTGGTGGAAATCCCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGaa        <  2:1984164/93‑1 (MQ=255)
                                                                                  cTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGaa        >  1:523232/1‑93 (MQ=255)
                                                                                  cTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGaa        <  1:4107805/93‑1 (MQ=255)
                                                                                   ctCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAAc       <  1:2781184/92‑1 (MQ=255)
                                                                                    tCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACg      >  1:5673388/1‑93 (MQ=255)
                                                                                    tCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACg      >  1:4410148/1‑93 (MQ=255)
                                                                                     cGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGa     >  1:5195670/1‑93 (MQ=255)
                                                                                       acacCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGAtt   >  2:1807520/1‑93 (MQ=255)
                                                                                        cATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTg  >  2:5209026/1‑93 (MQ=255)
                                                                                          |                                                                                          
AGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTG  >  NC_000913/3257862‑3258042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGA  >  NC_000913/3257859‑3258045
                                                                                             |                                                                                             
GTCAGGTTAGGATATTTTTCCGGGTTCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACAC                                                                                               <  SRR4302543.4126378/94‑1 (MQ=60)
  CAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCC                                                                                             <  SRR4302543.6283716/94‑1 (MQ=60)
  CAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCC                                                                                             <  SRR4302543.5279686/94‑1 (MQ=60)
  CAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCC                                                                                             <  SRR4302543.1414402/94‑1 (MQ=60)
  CAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCC                                                                                             <  SRR4302543.6456300/94‑1 (MQ=60)
   AGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCG                                                                                            <  SRR4302543.7256336/94‑1 (MQ=60)
   AGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCG                                                                                            >  SRR4302543.5856891/1‑94 (MQ=60)
    GGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGC                                                                                           >  SRR4302543.5125190/1‑94 (MQ=60)
    GGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGC                                                                                           >  SRR4302543.2423418/1‑94 (MQ=60)
    GGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGC                                                                                           <  SRR4302543.6684466/94‑1 (MQ=60)
     GTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCT                                                                                          <  SRR4302543.4459764/94‑1 (MQ=60)
     GTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCT                                                                                          >  SRR4302543.1366907/1‑94 (MQ=60)
      TTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTT                                                                                         <  SRR4302543.5584202/94‑1 (MQ=60)
       TAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTC                                                                                        >  SRR4302543.3013919/1‑94 (MQ=60)
       TAGGATATTTTTCCGGGTGCTCGATAGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTC                                                                                        >  SRR4302543.4164854/1‑94 (MQ=60)
        AGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCG                                                                                       >  SRR4302543.535815/1‑94 (MQ=60)
        AGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCG                                                                                       <  SRR4302543.3146535/94‑1 (MQ=60)
        AGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCG                                                                                       >  SRR4302543.5103168/1‑94 (MQ=60)
         GGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGT                                                                                      >  SRR4302543.3424577/1‑94 (MQ=60)
          GATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTG                                                                                     >  SRR4302543.924415/1‑94 (MQ=60)
           ATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGG                                                                                    <  SRR4302543.6802979/94‑1 (MQ=60)
            TATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGT                                                                                   <  SRR4302543.5342800/94‑1 (MQ=60)
             ATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTG                                                                                  >  SRR4302543.1469618/1‑94 (MQ=60)
              attttccggtgtgTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGGGTTGACCGCCTTCGACACCCGCTTCGTGGTGG                                                                                 <  SRR4302543.2410174/81‑1 (MQ=60)
               TTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGA                                                                                <  SRR4302543.3663247/94‑1 (MQ=60)
               TTTTCCGGGTGCTCGATGGCATCAAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGA                                                                                >  SRR4302543.5540684/1‑94 (MQ=60)
                TTTCCGGGTTCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAA                                                                               <  SRR4302543.6419541/94‑1 (MQ=60)
                TTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAA                                                                               <  SRR4302543.7421679/94‑1 (MQ=60)
                  TCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAAT                                                                             <  SRR4302543.1266632/94‑1 (MQ=60)
                  TCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAAT                                                                             <  SRR4302543.6210172/94‑1 (MQ=60)
                   TCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATA                                                                            <  SRR4302543.2627087/94‑1 (MQ=60)
                   CCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATA                                                                            <  SRR4302543.1343105/94‑1 (MQ=60)
                    CGGGTGCTCGATGGCATCCAGCAGCATTTCCCTATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATAC                                                                           <  SRR4302543.1799049/94‑1 (MQ=60)
                    CGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATAC                                                                           <  SRR4302543.255350/94‑1 (MQ=60)
                    CGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATAC                                                                           >  SRR4302543.3171747/1‑94 (MQ=60)
                    CGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGGTTTGACCGCCTGCGACACCCGCTTCGGGGGGGAAATAC                                                                           >  SRR4302543.1447008/1‑94 (MQ=60)
                     GGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACC                                                                          <  SRR4302543.4039159/94‑1 (MQ=60)
                       GTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCA                                                                        >  SRR4302543.2783967/1‑94 (MQ=60)
                       GTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCA                                                                        <  SRR4302543.3646548/94‑1 (MQ=60)
                        TGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCAT                                                                       >  SRR4302543.3177302/1‑94 (MQ=60)
                          CTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCC                                                                     <  SRR4302543.1846205/94‑1 (MQ=60)
                          CTCGAGGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCC                                                                     <  SRR4302543.4954456/94‑1 (MQ=60)
                           TCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCA                                                                    >  SRR4302543.3486905/1‑94 (MQ=60)
                           TCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCA                                                                    >  SRR4302543.3291492/1‑94 (MQ=60)
                           TCGATCGCATCCAGCAGCATTTCCCGCTTCATTACGTTTACGTTGACGTGTTGACCGCCTTCGCCACCCGCTTCGTGGTGGAAATACCTATCCC                                                                    >  SRR4302543.3486906/1‑94 (MQ=60)
                            CGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAG                                                                   <  SRR4302543.6724597/94‑1 (MQ=60)
                            CGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAG                                                                   <  SRR4302543.7418694/94‑1 (MQ=60)
                            CGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAG                                                                   <  SRR4302543.3018076/94‑1 (MQ=60)
                            CGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAC                                                                   >  SRR4302543.313369/1‑94 (MQ=60)
                             GATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGC                                                                  <  SRR4302543.3294181/94‑1 (MQ=60)
                             GATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGC                                                                  >  SRR4302543.2654582/1‑94 (MQ=60)
                              ATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCA                                                                 <  SRR4302543.455404/94‑1 (MQ=60)
                               TGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAG                                                                <  SRR4302543.7539333/94‑1 (MQ=60)
                               TGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAG                                                                <  SRR4302543.1543818/94‑1 (MQ=60)
                                gtgaacccgcaagaattcccgagtcattacgtgtgcgtgGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGG                                                               <  SRR4302543.1896520/55‑1 (MQ=60)
                                 GCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGC                                                              <  SRR4302543.3487017/94‑1 (MQ=60)
                                  CATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCC                                                             >  SRR4302543.7822953/1‑94 (MQ=60)
                                  CATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCC                                                             >  SRR4302543.1725896/1‑94 (MQ=60)
                                   ATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCG                                                            >  SRR4302543.1633080/1‑94 (MQ=60)
                                     CCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGAC                                                          >  SRR4302543.6536626/1‑94 (MQ=60)
                                     CCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGAC                                                          <  SRR4302543.845619/94‑1 (MQ=60)
                                     CCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGAC                                                          >  SRR4302543.6355403/1‑94 (MQ=60)
                                      CAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCTTGGTGGAAATACCCATCCAGCAGGCCGACA                                                         >  SRR4302543.6059301/1‑94 (MQ=60)
                                      CAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACA                                                         >  SRR4302543.5143338/1‑94 (MQ=60)
                                        GCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAG                                                       <  SRR4302543.2907822/94‑1 (MQ=60)
                                         CAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGG                                                      <  SRR4302543.7386502/94‑1 (MQ=60)
                                         CAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGG                                                      <  SRR4302543.1591004/94‑1 (MQ=60)
                                         CAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGG                                                      <  SRR4302543.98653/94‑1 (MQ=60)
                                          AGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGT                                                     <  SRR4302543.4544058/94‑1 (MQ=60)
                                           GCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTT                                                    <  SRR4302543.1842312/94‑1 (MQ=60)
                                           GCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTT                                                    <  SRR4302543.7990379/94‑1 (MQ=60)
                                           ACATTTCCCGATTCAGTACGTTGACGGTGGGGTGGTGACCGCCTTCGGCACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTT                                                    <  SRR4302543.1842311/94‑1 (MQ=60)
                                            CATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTG                                                   <  SRR4302543.1996184/94‑1 (MQ=60)
                                            CATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTG                                                   >  SRR4302543.7401359/1‑94 (MQ=60)
                                            CATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTG                                                   >  SRR4302543.3667149/1‑94 (MQ=60)
                                            CATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTG                                                   >  SRR4302543.3886034/1‑94 (MQ=60)
                                            CATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTG                                                   >  SRR4302543.3949657/1‑94 (MQ=60)
                                             ATTTCCCGATTCTTTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGG                                                  <  SRR4302543.737610/94‑1 (MQ=60)
                                             ATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGG                                                  <  SRR4302543.7294291/94‑1 (MQ=60)
                                             ATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGG                                                  >  SRR4302543.1371378/1‑94 (MQ=60)
                                             ATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACAGCCTTCGACACCCGATTCGTGGCGGGAATACCCATCCAGCAGGCCGACAAGGTTGG                                                  >  SRR4302543.4145042/1‑94 (MQ=60)
                                              TTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGT                                                 >  SRR4302543.1207300/1‑94 (MQ=60)
                                               TTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTT                                                <  SRR4302543.2997821/94‑1 (MQ=60)
                                               TTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTT                                                <  SRR4302543.1492859/94‑1 (MQ=60)
                                                TCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTT                                               <  SRR4302543.1154571/94‑1 (MQ=60)
                                                TCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTT                                               <  SRR4302543.4674769/94‑1 (MQ=60)
                                                 ccacctccagtccgctgaggtgtgcGTGTTGCCCGCCTTCGACACCCGCGTCGTGGTGGAAATCCCCATCCAACAGGCCGACAAGGTTGGTTTT                                              <  SRR4302543.6743573/69‑1 (MQ=56)
                                                 CCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTT                                              <  SRR4302543.3191217/94‑1 (MQ=60)
                                                  CCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTA                                             <  SRR4302543.6553602/94‑1 (MQ=60)
                                                   CGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTAC                                            >  SRR4302543.7604914/1‑94 (MQ=60)
                                                   CGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTAC                                            <  SRR4302543.1938602/94‑1 (MQ=60)
                                                    GATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACG                                           >  SRR4302543.712555/1‑94 (MQ=60)
                                                    GATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACG                                           <  SRR4302543.3410101/94‑1 (MQ=60)
                                                     ATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGT                                          >  SRR4302543.135880/1‑94 (MQ=60)
                                                     ATTCATTACGTTGACGTTGAGGTGTTGACCGCCATCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGT                                          >  SRR4302543.7570979/1‑94 (MQ=60)
                                                      TTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTA                                         >  SRR4302543.5133930/1‑94 (MQ=60)
                                                      TTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTA                                         <  SRR4302543.542543/94‑1 (MQ=60)
                                                       cgatctccactctGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTAC                                        <  SRR4302543.2319998/81‑1 (MQ=60)
                                                        CATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACT                                       <  SRR4302543.4983427/94‑1 (MQ=60)
                                                        CATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACT                                       >  SRR4302543.2505822/1‑94 (MQ=60)
                                                        CATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACT                                       >  SRR4302543.32751/1‑94 (MQ=60)
                                                        CATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACT                                       >  SRR4302543.2162824/1‑94 (MQ=60)
                                                        CATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACT                                       <  SRR4302543.2987461/94‑1 (MQ=60)
                                                         ATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTG                                      <  SRR4302543.725135/94‑1 (MQ=60)
                                                          TTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGG                                     <  SRR4302543.4901681/94‑1 (MQ=60)
                                                          TTACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGG                                     <  SRR4302543.1593054/94‑1 (MQ=60)
                                                            ACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGAT                                   <  SRR4302543.6717203/94‑1 (MQ=60)
                                                            ACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGAT                                   >  SRR4302543.821643/1‑94 (MQ=60)
                                                            ACGTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGAT                                   >  SRR4302543.3007786/1‑94 (MQ=60)
                                                              GTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCT                                 >  SRR4302543.3783112/1‑94 (MQ=60)
                                                              GTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCT                                 >  SRR4302543.2130770/1‑94 (MQ=60)
                                                              GTTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCT                                 <  SRR4302543.6052807/94‑1 (MQ=60)
                                                               TTGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTT                                >  SRR4302543.2686199/1‑94 (MQ=60)
                                                                TGACGTTGAGGTGTTGTCCGCCTTGGCCACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTC                               <  SRR4302543.4068419/94‑1 (MQ=60)
                                                                TGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTC                               <  SRR4302543.7241934/94‑1 (MQ=60)
                                                                TGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTC                               >  SRR4302543.3918614/1‑94 (MQ=60)
                                                                TGACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTC                               <  SRR4302543.3204280/94‑1 (MQ=60)
                                                                 GACGTTGAGGTGTTGACCGCCTTCGACCCCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCT                              <  SRR4302543.8745/94‑1 (MQ=60)
                                                                 GACGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAGATACCCATCCAGCAGGCCGACAAGGTTGGCTTTACGTACTGGATCTTCT                              >  SRR4302543.5610664/1‑94 (MQ=60)
                                                                   CGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTT                            <  SRR4302543.146145/94‑1 (MQ=60)
                                                                   CGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTT                            >  SRR4302543.6087890/1‑94 (MQ=60)
                                                                   CGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTT                            >  SRR4302543.291452/1‑94 (MQ=60)
                                                                   CGTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTT                            <  SRR4302543.6607410/94‑1 (MQ=60)
                                                                    GTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGCAATACCCATCCAGCAAGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTC                           >  SRR4302543.2703136/1‑94 (MQ=60)
                                                                    GTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTG                           >  SRR4302543.2703134/1‑94 (MQ=60)
                                                                    GTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTG                           >  SRR4302543.2703135/1‑94 (MQ=60)
                                                                    GTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTG                           >  SRR4302543.456149/1‑94 (MQ=60)
                                                                    GTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTG                           <  SRR4302543.1999023/94‑1 (MQ=60)
                                                                    GTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTG                           >  SRR4302543.5301630/1‑94 (MQ=60)
                                                                    GTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTG                           >  SRR4302543.6834607/1‑94 (MQ=60)
                                                                    GTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCCTCTTTG                           >  SRR4302543.6630129/1‑94 (MQ=60)
                                                                    GTTGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATAACCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTG                           >  SRR4302543.7052563/1‑94 (MQ=60)
                                                                    GTTGAGGGGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTG                           <  SRR4302543.3128285/94‑1 (MQ=60)
                                                                      TGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCC                         >  SRR4302543.117588/1‑94 (MQ=60)
                                                                      TGAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCC                         >  SRR4302543.3626427/1‑94 (MQ=60)
                                                                       GAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCC                        <  SRR4302543.6245514/94‑1 (MQ=60)
                                                                       GAGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCC                        <  SRR4302543.6245515/94‑1 (MQ=60)
                                                                        AGGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCA                       <  SRR4302543.788700/94‑1 (MQ=60)
                                                                        AGGTGTTGACCGCCTTCCACACCCGCTTCGTCGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCA                       <  SRR4302543.788695/94‑1 (MQ=60)
                                                                         GGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTCCGTACTGGATCTTCTTTGCCCAG                      <  SRR4302543.1414080/94‑1 (MQ=60)
                                                                         GGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAG                      <  SRR4302543.5248047/94‑1 (MQ=60)
                                                                         GGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAG                      <  SRR4302543.1677047/94‑1 (MQ=60)
                                                                         GGTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAG                      >  SRR4302543.6098910/1‑94 (MQ=60)
                                                                          GTGTTGACCGCCTTCTACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGC                     <  SRR4302543.906974/94‑1 (MQ=60)
                                                                          GTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGC                     <  SRR4302543.381253/94‑1 (MQ=60)
                                                                          GTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGC                     <  SRR4302543.855427/94‑1 (MQ=60)
                                                                          GTGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGC                     >  SRR4302543.1467838/1‑94 (MQ=60)
                                                                           TGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCG                    >  SRR4302543.1285571/1‑94 (MQ=60)
                                                                           TGTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCG                    <  SRR4302543.1808662/94‑1 (MQ=60)
                                                                            GTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGC                   >  SRR4302543.4680468/1‑94 (MQ=60)
                                                                            GTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGC                   >  SRR4302543.2603756/1‑94 (MQ=60)
                                                                            GTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGC                   >  SRR4302543.1858512/1‑94 (MQ=60)
                                                                            GTTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGC                   >  SRR4302543.2603553/1‑94 (MQ=60)
                                                                             TTGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGCCAAGGTTGGTTTTACGTACTGGATCTTTTTTGCTCAGCGCC                  >  SRR4302543.2712213/1‑94 (MQ=60)
                                                                              TGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCG                 <  SRR4302543.7815671/94‑1 (MQ=60)
                                                                              TGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCG                 <  SRR4302543.7815670/94‑1 (MQ=60)
                                                                              TGACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCG                 >  SRR4302543.1227609/1‑94 (MQ=60)
                                                                               GACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGC                >  SRR4302543.7272135/1‑94 (MQ=60)
                                                                               GACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGC                <  SRR4302543.1975699/94‑1 (MQ=60)
                                                                                ACCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCA               >  SRR4302543.5645371/1‑94 (MQ=60)
                                                                                 CCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAG              <  SRR4302543.4453411/94‑1 (MQ=60)
                                                                                 CCGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAG              <  SRR4302543.3393156/94‑1 (MQ=60)
                                                                                  CGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGG             >  SRR4302543.6751200/1‑94 (MQ=60)
                                                                                  CGCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGG             <  SRR4302543.3029156/94‑1 (MQ=60)
                                                                                   GCCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGA            >  SRR4302543.6856244/1‑94 (MQ=60)
                                                                                    CCTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAA           >  SRR4302543.528080/1‑94 (MQ=60)
                                                                                     CTTCGACTCCCGCTTCGTGGTGGAAATACCCGTCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAAC          <  SRR4302543.443132/94‑1 (MQ=60)
                                                                                     CTTCGACCCCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAAC          <  SRR4302543.6636800/94‑1 (MQ=60)
                                                                                     CTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAAC          <  SRR4302543.1839467/94‑1 (MQ=60)
                                                                                     CTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAAC          <  SRR4302543.1187679/94‑1 (MQ=60)
                                                                                     CTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAAC          <  SRR4302543.461695/94‑1 (MQ=60)
                                                                                     CTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAAC          <  SRR4302543.5551020/94‑1 (MQ=60)
                                                                                     CTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAAC          >  SRR4302543.3732998/1‑94 (MQ=60)
                                                                                     CTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAAC          <  SRR4302543.4144761/94‑1 (MQ=60)
                                                                                     CTTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAAC          <  SRR4302543.3903141/94‑1 (MQ=60)
                                                                                      TTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACG         >  SRR4302543.4450121/1‑94 (MQ=60)
                                                                                      TTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACG         >  SRR4302543.5725170/1‑94 (MQ=60)
                                                                                      TTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACG         >  SRR4302543.4742513/1‑94 (MQ=60)
                                                                                      TTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACG         <  SRR4302543.2088461/94‑1 (MQ=60)
                                                                                      TTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACG         >  SRR4302543.5258458/1‑94 (MQ=60)
                                                                                      TTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGCTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACG         >  SRR4302543.4093167/1‑94 (MQ=60)
                                                                                      CTCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACG         <  SRR4302543.2806536/94‑1 (MQ=60)
                                                                                       TCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGA        >  SRR4302543.5242515/1‑94 (MQ=60)
                                                                                       TCGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGA        <  SRR4302543.6190840/94‑1 (MQ=60)
                                                                                        CGACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGAT       <  SRR4302543.157493/94‑1 (MQ=60)
                                                                                         GACACCCGCTTCGTGGTGGAAATCCCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATT      <  SRR4302543.1974900/94‑1 (MQ=60)
                                                                                         GACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATT      <  SRR4302543.7991093/94‑1 (MQ=60)
                                                                                         GACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATT      <  SRR4302543.6737159/94‑1 (MQ=60)
                                                                                         GACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATT      <  SRR4302543.2641976/94‑1 (MQ=60)
                                                                                         GACACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATT      <  SRR4302543.2732995/94‑1 (MQ=60)
                                                                                           CACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGA    <  SRR4302543.5380292/94‑1 (MQ=60)
                                                                                           CACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGA    <  SRR4302543.5380293/94‑1 (MQ=60)
                                                                                           CACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGA    >  SRR4302543.6926005/1‑94 (MQ=60)
                                                                                           CACCCGCTTCGTGGCGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGA    >  SRR4302543.7508600/1‑94 (MQ=60)
                                                                                            ACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAG   >  SRR4302543.4561739/1‑94 (MQ=60)
                                                                                            ACCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAG   <  SRR4302543.4457114/94‑1 (MQ=60)
                                                                                            ACCCGCTGCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAG   <  SRR4302543.3538681/94‑1 (MQ=60)
                                                                                             CCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGA  >  SRR4302543.1705303/1‑94 (MQ=60)
                                                                                             CCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGA  >  SRR4302543.3417717/1‑94 (MQ=60)
                                                                                             |                                                                                             
GTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGA  >  NC_000913/3257859‑3258045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑