Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,262,568 T→C T31A (ACG→GCG)  tdcC ← TdcC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,262,5680TC99.5% 607.1 / ‑5.1 196T31A (ACG→GCG) tdcCTdcC
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (102/93);  total (102/94)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.57e-01

Only 100 of 204 total aligned reads displayed.

GTATGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGTACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTATCTGAAGTACTCA  >  NC_000913/3262479‑3262657
                                                                                         |                                                                                         
gTATGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCAcc                                                                                        <  2:2161453/93‑1 (MQ=255)
gTATGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCAcc                                                                                        <  1:699471/93‑1 (MQ=255)
  aTGCCAATACGAAGATCAGAATAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCTCCaa                                                                                      <  2:3004586/93‑1 (MQ=255)
  aTGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCaa                                                                                      <  1:4210354/93‑1 (MQ=255)
   tGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCaaa                                                                                     >  2:742988/1‑93 (MQ=255)
   tGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCaaa                                                                                     >  2:4677559/1‑93 (MQ=255)
    gCCAATACCAACATCAGAAGAATCGGTATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAAc                                                                                    <  2:4608675/93‑1 (MQ=255)
    gCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAAc                                                                                    <  1:1011544/93‑1 (MQ=255)
     ccAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACa                                                                                   <  2:2289557/93‑1 (MQ=255)
      cAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACaa                                                                                  >  2:4140002/1‑93 (MQ=255)
       aaTACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAg                                                                                 >  2:1073678/1‑93 (MQ=255)
       aaTACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAg                                                                                 <  1:4440139/93‑1 (MQ=255)
         tACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGcc                                                                               <  2:52233/93‑1 (MQ=255)
          aCCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCt                                                                              >  2:2571205/1‑93 (MQ=255)
           ccAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTa                                                                             >  2:1884598/1‑93 (MQ=255)
            cAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTaa                                                                            <  2:18313/93‑1 (MQ=255)
            cAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTaa                                                                            <  2:3082564/93‑1 (MQ=255)
            cAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTaa                                                                            <  1:775403/93‑1 (MQ=255)
             aaCATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAAc                                                                           >  2:66360/1‑93 (MQ=255)
             aaCATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGAATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAAc                                                                           >  1:2221152/1‑93 (MQ=255)
              aCATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGCACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACg                                                                          <  1:2511735/93‑1 (MQ=255)
              aCATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACg                                                                          >  1:4585228/1‑93 (MQ=255)
                aTCAGAAGAATCGTGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTc                                                                        <  2:2924989/93‑1 (MQ=255)
                aTCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTc                                                                        >  2:2684574/1‑93 (MQ=255)
                 tCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTcc                                                                       >  1:535304/1‑93 (MQ=255)
                  cAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTTCCTCACCAAACAAGCCTAACGTCCa                                                                      <  1:3793282/93‑1 (MQ=255)
                  cAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCa                                                                      <  1:936677/93‑1 (MQ=255)
                  cAGAAGAATCGGGATCAGTCCGCCAAAACCGGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCa                                                                      <  2:3711571/93‑1 (MQ=255)
                    gaagaaTCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCAtg                                                                    >  1:2303839/1‑93 (MQ=255)
                       gaaTCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAATAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTgg                                                                 >  2:4491136/1‑93 (MQ=255)
                       gaaTCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTgg                                                                 >  1:4144658/1‑93 (MQ=255)
                        aaTCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGt                                                                <  1:1335556/93‑1 (MQ=255)
                          tCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTAt                                                              >  1:4420418/1‑93 (MQ=255)
                           cGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATc                                                             <  1:4533281/93‑1 (MQ=255)
                            gggATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCt                                                            >  2:3944655/1‑93 (MQ=255)
                             ggATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTg                                                           >  1:2662378/1‑93 (MQ=255)
                             ggATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTg                                                           >  1:2616274/1‑93 (MQ=255)
                              gATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGa                                                          <  2:3423504/93‑1 (MQ=255)
                               aTCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGAt                                                         >  1:301414/1‑93 (MQ=255)
                                tCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGAtt                                                        >  1:3971266/1‑93 (MQ=255)
                                 cAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGAttt                                                       <  2:2239481/93‑1 (MQ=255)
                                  aGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTa                                                      >  1:596875/1‑93 (MQ=255)
                                  aGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTa                                                      <  2:2027978/93‑1 (MQ=255)
                                   ttcagCCAAAACCTGTGCGCATATGGCAGAACAGCACCCCGGCGCCGATTGCCGCCCCAAACAAGCCTAACGTCCATGGGGTATCTGATTTAc                                                     <  2:3476375/89‑1 (MQ=255)
                                    tCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACg                                                    >  1:1486904/1‑93 (MQ=255)
                                       gCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCa                                                 >  2:4640118/1‑93 (MQ=255)
                                       gCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCa                                                 >  2:4519998/1‑93 (MQ=255)
                                       gCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCa                                                 >  2:2412580/1‑93 (MQ=255)
                                         cAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAgg                                               >  1:3586841/1‑93 (MQ=255)
                                         cAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAgg                                               >  1:3230010/1‑93 (MQ=255)
                                         cAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAgg                                               >  2:2808510/1‑93 (MQ=255)
                                         cAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAgg                                               >  1:3551835/1‑93 (MQ=255)
                                          aaaaCCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGa                                              >  2:1936453/1‑93 (MQ=255)
                                          aaaaCCTGCGCGGATAGGGAAGAACAGCAACCCGGCGCCGATTGCCGCACCAAACAAGACTAACGTCCATGTGGTATCTGATTTACGCCAGGa                                              >  2:3277237/1‑93 (MQ=255)
                                           aaaCCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGAc                                             <  2:3529379/93‑1 (MQ=255)
                                            aaCCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACg                                            <  2:1036491/93‑1 (MQ=255)
                                             aCCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTAGCTGATTTACGCCAGGACGa                                           <  2:904900/93‑1 (MQ=255)
                                              ccTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGAt                                          <  1:4666566/93‑1 (MQ=255)
                                               cTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGAtt                                         <  2:2936791/93‑1 (MQ=255)
                                               cTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGAtt                                         <  1:1132102/93‑1 (MQ=255)
                                               cTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGAtt                                         >  2:2140989/1‑93 (MQ=255)
                                                tGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTg                                        >  2:1633857/1‑93 (MQ=255)
                                                   gcgGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGttt                                     <  2:4053921/93‑1 (MQ=255)
                                                   gcgGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGttt                                     >  1:4624744/1‑93 (MQ=255)
                                                     ggATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTg                                   >  2:766075/1‑93 (MQ=255)
                                                      gATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGt                                  >  1:3094879/1‑93 (MQ=255)
                                                        tAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTct                                >  1:3464457/1‑93 (MQ=255)
                                                        acgggAACAACATCACCCCGGCGCCGATTGCCGCACGAAACAAGCCTAACGTCCACGTGCTATCTGATCTACGCCAGGACGATTGTTTTGTct                                <  1:2085653/91‑1 (MQ=255)
                                                         aGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTctg                               <  2:515912/93‑1 (MQ=255)
                                                         aGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTctg                               <  1:989518/93‑1 (MQ=255)
                                                          gggAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTctgg                              >  2:2601494/1‑93 (MQ=255)
                                                          gggAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTctgg                              <  2:3835452/93‑1 (MQ=255)
                                                          gggAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTctgg                              <  1:3859180/93‑1 (MQ=255)
                                                             aagaaCAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTctggctg                           <  1:1914517/93‑1 (MQ=255)
                                                                    gCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACaa                    >  1:1575085/1‑93 (MQ=255)
                                                                     cACCCCGGCGCCGATTGCCGTACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAAt                   <  1:4114804/93‑1 (MQ=255)
                                                                     cACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAAt                   <  2:1546024/93‑1 (MQ=255)
                                                                     cACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAAt                   >  2:2522451/1‑93 (MQ=255)
                                                                     cACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAAt                   <  2:4357905/93‑1 (MQ=255)
                                                                       ccccGGCGCCTATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGc                 >  2:3386737/1‑93 (MQ=255)
                                                                        cccGGCGCCGATTGCCGCACCAAACAAGCCTATCGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCt                <  1:1941049/93‑1 (MQ=255)
                                                                         ccGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGTCTGGATACAATGCTa               <  2:3181627/93‑1 (MQ=255)
                                                                         ccGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTa               >  2:136046/1‑93 (MQ=255)
                                                                         ccGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTa               <  2:4255382/93‑1 (MQ=255)
                                                                         ccGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTa               >  2:4035825/1‑93 (MQ=255)
                                                                          cGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTAt              <  1:1156054/93‑1 (MQ=255)
                                                                           ggCGCCGATTGCCGCACTAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTATc             <  1:1248405/93‑1 (MQ=255)
                                                                           ggCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTATc             <  1:2359968/93‑1 (MQ=255)
                                                                              gcCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATAAAATGCTATCTGa          >  2:2719579/1‑93 (MQ=255)
                                                                               ccGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTATCTGaa         <  2:2197795/93‑1 (MQ=255)
                                                                                  aTTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTATCTGAAGTa      >  1:1197331/1‑93 (MQ=255)
                                                                                   ttGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTATCTGAAGTAc     <  1:3000383/93‑1 (MQ=255)
                                                                                   ttGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGCTTTGTCTGGCTGGATACAATGCTATCTGAAGTAc     >  2:2376545/1‑93 (MQ=255)
                                                                                    tGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTATCTGAAGTACt    <  1:3641414/93‑1 (MQ=255)
                                                                                    tGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTATCTGAAGTACt    <  2:1062204/93‑1 (MQ=255)
                                                                                     gCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTATCTGAAGTACTc   >  2:1012797/1‑93 (MQ=255)
                                                                                      ccGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTATCTGAAGTACTCa  <  1:3382064/93‑1 (MQ=255)
                                                                                      ccGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTATCTGAAGTACTCa  <  1:2131373/93‑1 (MQ=255)
                                                                                      ccGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTATCTGAAGTACTCa  <  2:2762961/93‑1 (MQ=255)
                                                                                      ccGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTATCTGAAGTACTCa  <  1:126778/93‑1 (MQ=255)
                                                                                         |                                                                                         
GTATGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGTACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTATCTGAAGTACTCA  >  NC_000913/3262479‑3262657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGGTATGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGTACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTATCTGAAGTACTCATATC  >  NC_000913/3262477‑3262661
                                                                                           |                                                                                             
GGGTATGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCAC                                                                                             <  SRR4302535.3931691/94‑1 (MQ=60)
  GTATGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCA                                                                                           <  SRR4302535.704379/94‑1 (MQ=60)
    ATGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAA                                                                                         <  SRR4302535.4239173/94‑1 (MQ=60)
      GCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACA                                                                                       <  SRR4302535.1018731/94‑1 (MQ=60)
       CCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAA                                                                                      <  SRR4302535.492449/94‑1 (MQ=60)
        CAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAG                                                                                     >  SRR4302535.2592467/1‑94 (MQ=60)
         AATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGC                                                                                    <  SRR4302535.4470576/94‑1 (MQ=60)
          ATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCC                                                                                   >  SRR4302535.4175214/1‑94 (MQ=60)
              CAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAAC                                                                               <  SRR4302535.780857/94‑1 (MQ=60)
              CAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAAC                                                                               <  SRR4302535.3027352/94‑1 (MQ=60)
              CAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGAATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAAC                                                                               >  SRR4302535.2236967/1‑94 (MQ=60)
               AACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACG                                                                              <  SRR4302535.1267394/94‑1 (MQ=60)
               AACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACG                                                                              >  SRR4302535.4616452/1‑94 (MQ=60)
               AACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACG                                                                              >  SRR4302535.3642854/1‑94 (MQ=60)
                ACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGCACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGT                                                                             <  SRR4302535.2529840/94‑1 (MQ=60)
                  ATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCC                                                                           >  SRR4302535.116322/1‑94 (MQ=60)
                  ATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCC                                                                           >  SRR4302535.539044/1‑94 (MQ=60)
                  ATCAGAAGAATCGGGAGCAGTCCGCTATATCCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGttaaacgatc                                                                           >  SRR4302535.4713007/1‑84 (MQ=60)
                   TCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCA                                                                          >  SRR4302535.1002767/1‑94 (MQ=60)
                   TCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCA                                                                          >  SRR4302535.2268588/1‑94 (MQ=60)
                    CAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTTCCTCACCAAACAAGCCTAACGTCCAT                                                                         <  SRR4302535.3819435/94‑1 (MQ=60)
                    CAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCAT                                                                         <  SRR4302535.943312/94‑1 (MQ=60)
                     AGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATG                                                                        >  SRR4302535.2320318/1‑94 (MQ=60)
                       AAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTG                                                                      <  SRR4302535.1929401/94‑1 (MQ=60)
                        AGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGG                                                                     >  SRR4302535.4173039/1‑94 (MQ=60)
                        AGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGG                                                                     <  SRR4302535.3852045/94‑1 (MQ=60)
                          AATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTA                                                                   <  SRR4302535.1345084/94‑1 (MQ=60)
                          AATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTA                                                                   <  SRR4302535.1279513/94‑1 (MQ=60)
                           ATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTAT                                                                  >  SRR4302535.4450684/1‑94 (MQ=60)
                           ATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTAT                                                                  <  SRR4302535.8087/94‑1 (MQ=60)
                             CGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCT                                                                <  SRR4302535.4564306/94‑1 (MQ=60)
                             CGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCT                                                                >  SRR4302535.3407490/1‑94 (MQ=60)
                              GGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTG                                                               >  SRR4302535.4631105/1‑94 (MQ=60)
                              GGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTG                                                               >  SRR4302535.2635033/1‑94 (MQ=60)
                              GGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTG                                                               >  SRR4302535.2681442/1‑94 (MQ=60)
                               GGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGAGTGCCGCACCACACAAGCCTAACGTCCATGTGGTATCTGA                                                              >  SRR4302535.4149164/1‑94 (MQ=60)
                                GATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGAT                                                             >  SRR4302535.303515/1‑94 (MQ=60)
                                 ATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATT                                                            >  SRR4302535.3998390/1‑94 (MQ=60)
                                   CAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTA                                                          >  SRR4302535.601055/1‑94 (MQ=60)
                                     GTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACG                                                        >  SRR4302535.1497551/1‑94 (MQ=60)
                                         GCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAG                                                    <  SRR4302535.3803384/94‑1 (MQ=60)
                                          CCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGG                                                   >  SRR4302535.3576483/1‑94 (MQ=60)
                                          CCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGG                                                   >  SRR4302535.3611718/1‑94 (MQ=60)
                                          CCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGG                                                   >  SRR4302535.3252400/1‑94 (MQ=60)
                                           CAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGA                                                  >  SRR4302535.1231567/1‑94 (MQ=60)
                                           CAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGA                                                  >  SRR4302535.2123139/1‑94 (MQ=60)
                                           CAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGA                                                  >  SRR4302535.3930417/1‑94 (MQ=60)
                                             AAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACG                                                >  SRR4302535.1512910/1‑94 (MQ=60)
                                             AAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACG                                                <  SRR4302535.2019577/94‑1 (MQ=60)
                                              AACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCTATGTGGTATCTGATTTACGCCAGGACGA                                               >  SRR4302535.4681510/1‑94 (MQ=60)
                                               ACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGAT                                              >  SRR4302535.4584740/1‑94 (MQ=60)
                                               ACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGAT                                              >  SRR4302535.4455500/1‑94 (MQ=60)
                                               ACCTGCACGTATAGGGAAGAACAGCACCCCGACTCCTATTGCCGCACCAAACAAGCCTAACGTCCACGTGGTATCTGATTTCCGCCAGGACGGT                                              <  SRR4302535.1293800/94‑1 (MQ=60)
                                                CCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATT                                             <  SRR4302535.4698109/94‑1 (MQ=60)
                                                 CTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTG                                            <  SRR4302535.1140184/94‑1 (MQ=60)
                                                 CTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTG                                            >  SRR4302535.3567244/1‑94 (MQ=60)
                                                  TGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGT                                           >  SRR4302535.727563/1‑94 (MQ=60)
                                                    CGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTT                                         >  SRR4302535.4656146/1‑94 (MQ=60)
                                                    CGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTT                                         <  SRR4302535.589838/94‑1 (MQ=60)
                                                       GGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGT                                      >  SRR4302535.3116429/1‑94 (MQ=60)
                                                       GGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGT                                      <  SRR4302535.236864/94‑1 (MQ=60)
                                                        GATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTC                                     >  SRR4302535.3190793/1‑94 (MQ=60)
                                                         ATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCT                                    >  SRR4302535.4470784/1‑94 (MQ=60)
                                                         ATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCT                                    >  SRR4302535.3572348/1‑94 (MQ=60)
                                                         ATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCT                                    >  SRR4302535.3488536/1‑94 (MQ=60)
                                                          acGGGAACAACATCACCCCGGCGCCGATTGCCGCACGAAACAAGCCTAACGTCCACGTGCTATCTGATCTACGCCAGGACGATTGTTTTGTCTG                                   <  SRR4302535.2100673/92‑1 (MQ=60)
                                                          TAGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTG                                   <  SRR4302535.985411/94‑1 (MQ=60)
                                                           AGGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGG                                  <  SRR4302535.996544/94‑1 (MQ=60)
                                                            GGGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGC                                 <  SRR4302535.3885740/94‑1 (MQ=60)
                                                             GGAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCT                                >  SRR4302535.1475274/1‑94 (MQ=60)
                                                              GAAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTG                               >  SRR4302535.2433152/1‑94 (MQ=60)
                                                               AAGAACAGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGG                              <  SRR4302535.1928313/94‑1 (MQ=60)
                                                                     AGCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAA                        >  SRR4302535.1586452/1‑94 (MQ=60)
                                                                      GCACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAAT                       <  SRR4302535.3584182/94‑1 (MQ=60)
                                                                       CACCCCGGCGCCGATTGCCGTACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATG                      <  SRR4302535.4142971/94‑1 (MQ=60)
                                                                       CACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATG                      <  SRR4302535.2192709/94‑1 (MQ=60)
                                                                        ACCCCGGCGCGGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGC                     <  SRR4302535.4080720/94‑1 (MQ=60)
                                                                        ACCCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGC                     <  SRR4302535.1042779/94‑1 (MQ=60)
                                                                          CCCGGCGCCGATTGCCGCACCAAACAAGCCTATCGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTA                   <  SRR4302535.1955035/94‑1 (MQ=60)
                                                                          CCCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTA                   >  SRR4302535.4412367/1‑94 (MQ=60)
                                                                           CCGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTAT                  <  SRR4302535.160175/94‑1 (MQ=60)
                                                                            CGGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTATC                 <  SRR4302535.1164313/94‑1 (MQ=60)
                                                                             GGCGCCGATTGCCGCACTAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTATCT                <  SRR4302535.1257314/94‑1 (MQ=60)
                                                                             GGCGCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTATCT                <  SRR4302535.2376904/94‑1 (MQ=60)
                                                                                GCCGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTATCTGAA             >  SRR4302535.3388965/1‑94 (MQ=60)
                                                                                  CGATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTATCTGAAGT           <  SRR4302535.1185309/94‑1 (MQ=60)
                                                                                   GATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTATCTGAAGTA          <  SRR4302535.664997/94‑1 (MQ=60)
                                                                                   GATTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTATCTGAAGTA          >  SRR4302535.1205905/1‑94 (MQ=60)
                                                                                     TTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTATCTGAAGTACT        <  SRR4302535.3021417/94‑1 (MQ=60)
                                                                                     TTGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTATCTGAAGTACT        <  SRR4302535.2718331/94‑1 (MQ=60)
                                                                                      TGCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTATCTGAAGTACTC       <  SRR4302535.3666636/94‑1 (MQ=60)
                                                                                       GCCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTATCTGAAGTACTCA      <  SRR4302535.2637898/94‑1 (MQ=60)
                                                                                       GCCGCACCAAACAAGCCCAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTATCTGAAGTACTCA      <  SRR4302535.3106603/94‑1 (MQ=60)
                                                                                        CCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTATCTGAAGTACTCAT     <  SRR4302535.3405576/94‑1 (MQ=60)
                                                                                        CCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTATCTGAAGTACTCAT     <  SRR4302535.2146738/94‑1 (MQ=60)
                                                                                        CCGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTATCTGAAGTACTCAT     <  SRR4302535.127671/94‑1 (MQ=60)
                                                                                         CGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTATCTGAAGTACTCATA    <  SRR4302535.2086242/94‑1 (MQ=60)
                                                                                         CGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTATCTGAAGTACTCATA    <  SRR4302535.3425110/94‑1 (MQ=60)
                                                                                         CGCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTATCTGAAGTACTCATA    >  SRR4302535.2082494/1‑94 (MQ=60)
                                                                                          GCACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTATCTGAAGTACTCATAT   <  SRR4302535.913856/94‑1 (MQ=60)
                                                                                           CACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTATCTGAAGTACTCATATC  >  SRR4302535.4211272/1‑94 (MQ=60)
                                                                                           CACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTATCTGAAGTACTCATATC  <  SRR4302535.3416791/94‑1 (MQ=60)
                                                                                           |                                                                                             
GGGTATGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGTACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACGATTGTTTTGTCTGGCTGGATACAATGCTATCTGAAGTACTCATATC  >  NC_000913/3262477‑3262661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑