Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,279,197 A→C R145S (AGA→AGC)  agaA → putative N‑acetylgalactosamine‑6‑phosphate deacetylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,279,1970AC100.0% 671.6 / NA 213R145S (AGA→AGC) agaAputative N‑acetylgalactosamine‑6‑phosphate deacetylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (95/118);  total (95/118)

Only 100 of 225 total aligned reads displayed.

GGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGAGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGG  >  NC_000913/3279107‑3279286
                                                                                          |                                                                                         
ggAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGt                                                                                         <  1:719930/93‑1 (MQ=255)
ggAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGt                                                                                         >  2:3984788/1‑93 (MQ=255)
ggAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGt                                                                                         <  1:2659455/93‑1 (MQ=255)
 gAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTc                                                                                        >  2:367078/1‑93 (MQ=255)
   aGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGcgtcgt                                                                                      >  2:3609610/1‑93 (MQ=255)
    gCCATCCGTATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGtt                                                                                     >  2:3098579/1‑93 (MQ=255)
      catccatATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTgc                                                                                   <  2:3388426/93‑1 (MQ=255)
        tccatATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTgcgc                                                                                 >  2:784801/1‑93 (MQ=255)
        tccatATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTgcgc                                                                                 <  2:2300700/93‑1 (MQ=255)
         ccatATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCt                                                                                >  1:2361099/1‑93 (MQ=255)
         ccatATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCt                                                                                <  2:1769121/93‑1 (MQ=255)
         ccatATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCt                                                                                >  2:4093512/1‑93 (MQ=255)
            tatGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGa                                                                             <  2:4654802/93‑1 (MQ=255)
             atGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGAt                                                                            <  2:336192/93‑1 (MQ=255)
             atGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGAt                                                                            >  2:115779/1‑93 (MQ=255)
              tGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATa                                                                           >  2:2140050/1‑93 (MQ=255)
              tGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATa                                                                           <  2:4605591/93‑1 (MQ=255)
              tGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATa                                                                           <  2:2452864/93‑1 (MQ=255)
              tGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATa                                                                           <  2:2018288/93‑1 (MQ=255)
              tGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATa                                                                           <  2:5442/93‑1 (MQ=255)
               ggCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAg                                                                          >  1:2417258/1‑93 (MQ=255)
                gCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAACCCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGc                                                                         <  1:1924672/93‑1 (MQ=255)
                gCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGc                                                                         <  1:4244729/93‑1 (MQ=255)
                gCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGc                                                                         >  1:3152182/1‑93 (MQ=255)
                gCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGc                                                                         <  2:2908843/93‑1 (MQ=255)
                   tCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCggg                                                                      >  1:1915940/1‑93 (MQ=255)
                     gCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCt                                                                    <  1:1591728/93‑1 (MQ=255)
                      cTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTa                                                                   >  2:1932751/1‑93 (MQ=255)
                      cTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTa                                                                   <  2:3894490/93‑1 (MQ=255)
                       tGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTAc                                                                  >  1:2806470/1‑93 (MQ=255)
                       tGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTAc                                                                  <  1:4300486/93‑1 (MQ=255)
                       tGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTAc                                                                  >  2:2352852/1‑93 (MQ=255)
                        gCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACa                                                                 <  1:3584167/93‑1 (MQ=255)
                         cATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACAt                                                                <  1:2997795/93‑1 (MQ=255)
                          aTCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTCCAtg                                                               >  2:3783248/1‑93 (MQ=255)
                          aTCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACAtg                                                               >  2:2397608/1‑93 (MQ=255)
                          aTCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACAtg                                                               >  1:1970875/1‑93 (MQ=255)
                           tCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACAtgt                                                              >  1:4633253/1‑93 (MQ=255)
                               gcgcGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGcaa                                                          <  1:3679534/93‑1 (MQ=255)
                                cgcgAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGcaac                                                         <  1:3911280/93‑1 (MQ=255)
                                  cgAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGcaacaa                                                       <  2:756525/93‑1 (MQ=255)
                                   gAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACaaa                                                      <  1:1094149/93‑1 (MQ=255)
                                   gAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACaaa                                                      >  1:2327566/1‑93 (MQ=255)
                                    aaTGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAAt                                                     >  2:1296187/1‑93 (MQ=255)
                                    aaTGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAAt                                                     >  2:4211681/1‑93 (MQ=255)
                                     aTGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATc                                                    <  1:4347822/93‑1 (MQ=255)
                                     aTGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATc                                                    <  2:161244/93‑1 (MQ=255)
                                      tGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCt                                                   <  1:4068323/93‑1 (MQ=255)
                                      tGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCt                                                   <  2:2892273/93‑1 (MQ=255)
                                       gCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTg                                                  <  2:2682537/93‑1 (MQ=255)
                                        cTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTgg                                                 >  1:428024/1‑93 (MQ=255)
                                           ggTGTTGATGGTGTTCTGGGATCGCTTAACCCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGAtt                                              <  1:2310010/93‑1 (MQ=255)
                                            gtgtTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTc                                             <  1:1551972/93‑1 (MQ=255)
                                            gtgtTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTc                                             <  2:1523463/93‑1 (MQ=255)
                                            gtgtTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTc                                             <  1:2968240/93‑1 (MQ=255)
                                             tgtTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCa                                            >  1:4542607/1‑93 (MQ=255)
                                              gtTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAg                                           >  1:2855459/1‑93 (MQ=255)
                                              gtTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAg                                           <  1:2107838/93‑1 (MQ=255)
                                              ggtGATGGTGTTCTGGGATCGCTTAAACCGGGCAACCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAg                                           <  2:70224/91‑1 (MQ=255)
                                               ttGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAgg                                          >  1:1675237/1‑93 (MQ=255)
                                               ttGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAgg                                          <  2:4406171/93‑1 (MQ=255)
                                                 gATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGt                                        >  2:2822430/1‑93 (MQ=255)
                                                 gATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGt                                        >  2:2579093/1‑93 (MQ=255)
                                                  aTGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTc                                       >  1:481052/1‑93 (MQ=255)
                                                       gtTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTa                                  <  2:1071682/93‑1 (MQ=255)
                                                       gtTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTa                                  <  1:2295550/93‑1 (MQ=255)
                                                        ttCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAg                                 <  1:65345/93‑1 (MQ=255)
                                                        ttCGGGGATCGCTTAAGCCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAg                                 <  2:4510281/93‑1 (MQ=255)
                                                            gggATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTc                             <  1:3832956/93‑1 (MQ=255)
                                                            gggATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTc                             <  2:3241899/93‑1 (MQ=255)
                                                             ggATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCg                            >  1:2859898/1‑93 (MQ=255)
                                                                 cGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGtttt                        <  2:1359069/93‑1 (MQ=255)
                                                                  gCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTg                       >  1:1307766/1‑93 (MQ=255)
                                                                   cTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGa                      >  2:2988540/1‑93 (MQ=255)
                                                                      aaaCCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAg                   <  1:754012/93‑1 (MQ=255)
                                                                      aaaCCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAg                   <  1:706864/93‑1 (MQ=255)
                                                                       aaCCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGt                  >  1:3286861/1‑93 (MQ=255)
                                                                       aaCCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGt                  <  1:855685/93‑1 (MQ=255)
                                                                           gggCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGc              >  2:2218521/1‑93 (MQ=255)
                                                                           gggCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGc              <  1:2821972/93‑1 (MQ=255)
                                                                           gggCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGc              <  2:3479884/93‑1 (MQ=255)
                                                                           gggCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGc              >  2:1215872/1‑93 (MQ=255)
                                                                            ggCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCt             <  2:2621930/93‑1 (MQ=255)
                                                                              cAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTcc           <  1:3405886/93‑1 (MQ=255)
                                                                               aaaCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCt          >  1:1348332/1‑93 (MQ=255)
                                                                               aaaCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCt          <  1:1743849/93‑1 (MQ=255)
                                                                                aaCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCtt         <  2:1034409/93‑1 (MQ=255)
                                                                                aaCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCtt         <  2:2538124/93‑1 (MQ=255)
                                                                                 aCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCttt        >  1:2795155/1‑93 (MQ=255)
                                                                                  cgcgCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTa       <  1:53044/93‑1 (MQ=255)
                                                                                  cgcgCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTa       <  2:2812650/93‑1 (MQ=255)
                                                                                   gcgcCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTAt      <  2:3507777/93‑1 (MQ=255)
                                                                                    cgcCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTAtt     <  1:3362520/93‑1 (MQ=255)
                                                                                    cgcCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTAtt     <  2:4163705/93‑1 (MQ=255)
                                                                                    cgcCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTAtt     <  2:1393471/93‑1 (MQ=255)
                                                                                      ccAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTgg   <  1:1257707/93‑1 (MQ=255)
                                                                                      ccAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTgg   >  1:2483466/1‑93 (MQ=255)
                                                                                      ccAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTgg   <  1:3136467/93‑1 (MQ=255)
                                                                                      ccAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGGCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTgg   >  2:4562720/1‑93 (MQ=255)
                                                                                      ccAGCGTCGTTGCCCTAGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTgg   <  2:1464556/93‑1 (MQ=255)
                                                                                          |                                                                                         
GGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGAGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGG  >  NC_000913/3279107‑3279286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCGGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGAGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGCCTT  >  NC_000913/3279104‑3279290
                                                                                             |                                                                                             
TGCGGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGC                                                                                               <  SRR4302535.1049135/94‑1 (MQ=60)
TGCGGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGC                                                                                               >  SRR4302535.1006924/1‑94 (MQ=60)
TGCGGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGC                                                                                               <  SRR4302535.710946/94‑1 (MQ=60)
 GCGGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCG                                                                                              >  SRR4302535.4283502/1‑94 (MQ=60)
   GGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTC                                                                                            <  SRR4302535.724988/94‑1 (MQ=60)
   GGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTC                                                                                            <  SRR4302535.2678508/94‑1 (MQ=60)
      AGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTT                                                                                         <  SRR4302535.1759480/94‑1 (MQ=60)
          ATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGC                                                                                     >  SRR4302535.3390266/1‑94 (MQ=60)
           TCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCT                                                                                    >  SRR4302535.2378051/1‑94 (MQ=60)
           TCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCT                                                                                    <  SRR4302535.1334505/94‑1 (MQ=60)
            CCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTG                                                                                   >  SRR4302535.1298892/1‑94 (MQ=60)
             CATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGG                                                                                  >  SRR4302535.3812229/1‑94 (MQ=60)
               TATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGAT                                                                                >  SRR4302535.3317591/1‑94 (MQ=60)
                 TGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAG                                                                              <  SRR4302535.4388711/94‑1 (MQ=60)
                 TGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAG                                                                              >  SRR4302535.2434658/1‑94 (MQ=60)
                 TGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAG                                                                              <  SRR4302535.615102/94‑1 (MQ=60)
                 TGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAG                                                                              <  SRR4302535.3633515/94‑1 (MQ=60)
                  GGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGC                                                                             >  SRR4302535.2930879/1‑94 (MQ=60)
                  GGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGC                                                                             >  SRR4302535.3174088/1‑94 (MQ=60)
                  GGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATAGTGTTCTGGGATCGCTTAAACCGGGCAAACGCACCAGCGTCGTTGCGCTGGATAGC                                                                             <  SRR4302535.2741464/94‑1 (MQ=60)
                   GCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAACCCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCG                                                                            <  SRR4302535.1938542/94‑1 (MQ=60)
                   GCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCG                                                                            <  SRR4302535.4273785/94‑1 (MQ=60)
                    CGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGG                                                                           >  SRR4302535.4315044/1‑94 (MQ=60)
                    CGNCNNNGNATNCNGCGCGNANGCTGGGTGNTGATGGTGNTCNGGGATCGCTTAAANCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGG                                                                           <  SRR4302535.275043/94‑1 (MQ=60)
                     GTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGG                                                                          >  SRR4302535.1929747/1‑94 (MQ=60)
                     GTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGG                                                                          <  SRR4302535.2846760/94‑1 (MQ=60)
                      TCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGC                                                                         >  SRR4302535.4337992/1‑94 (MQ=60)
                        GCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTA                                                                       <  SRR4302535.1603212/94‑1 (MQ=60)
                         CTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTAC                                                                      >  SRR4302535.2826421/1‑94 (MQ=60)
                         CTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTAC                                                                      >  SRR4302535.4698425/1‑94 (MQ=60)
                          TGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACA                                                                     <  SRR4302535.4329915/94‑1 (MQ=60)
                           GCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACAT                                                                    <  SRR4302535.3609025/94‑1 (MQ=60)
                            CATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATG                                                                   <  SRR4302535.3018818/94‑1 (MQ=60)
                            CATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATG                                                                   >  SRR4302535.970755/1‑94 (MQ=60)
                            CATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATG                                                                   >  SRR4302535.1985070/1‑94 (MQ=60)
                             ATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGT                                                                  >  SRR4302535.4664686/1‑94 (MQ=60)
                              TCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAGACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTG                                                                 <  SRR4302535.2262080/94‑1 (MQ=60)
                              TCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTG                                                                 <  SRR4302535.2071587/94‑1 (MQ=60)
                               CCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGC                                                                <  SRR4302535.2314736/94‑1 (MQ=60)
                               CCGGCGCGAATCCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGC                                                                <  SRR4302535.3013001/94‑1 (MQ=60)
                                CGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCA                                                               <  SRR4302535.1242192/94‑1 (MQ=60)
                                 GGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAA                                                              >  SRR4302535.3182988/1‑94 (MQ=60)
                                  GCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAAC                                                             <  SRR4302535.3705001/94‑1 (MQ=60)
                                   CGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACA                                                            <  SRR4302535.3938059/94‑1 (MQ=60)
                                   CGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACA                                                            >  SRR4302535.279908/1‑94 (MQ=60)
                                     CGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAA                                                          >  SRR4302535.2344219/1‑94 (MQ=60)
                                     CGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAA                                                          >  SRR4302535.2332433/1‑94 (MQ=60)
                                     CGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAA                                                          >  SRR4302535.700126/1‑94 (MQ=60)
                                      GAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAAT                                                         <  SRR4302535.1101937/94‑1 (MQ=60)
                                      GAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAAT                                                         <  SRR4302535.2954610/94‑1 (MQ=60)
                                       AATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATC                                                        >  SRR4302535.3610811/1‑94 (MQ=60)
                                       AATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATC                                                        <  SRR4302535.720076/94‑1 (MQ=60)
                                        ATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCT                                                       <  SRR4302535.4377567/94‑1 (MQ=60)
                                        ATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCT                                                       <  SRR4302535.4552995/94‑1 (MQ=60)
                                         TGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTG                                                      <  SRR4302535.4096167/94‑1 (MQ=60)
                                         TGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTG                                                      <  SRR4302535.2821120/94‑1 (MQ=60)
                                          GCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGG                                                     >  SRR4302535.431001/1‑94 (MQ=60)
                                          GCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGG                                                     <  SRR4302535.1904181/94‑1 (MQ=60)
                                              GGTGTTGATGGTGTTCTGGGATCGCTTAACCCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTC                                                 <  SRR4302535.2326544/94‑1 (MQ=60)
                                               GTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCA                                                <  SRR4302535.1563152/94‑1 (MQ=60)
                                               GTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCA                                                >  SRR4302535.2878040/1‑94 (MQ=60)
                                               GTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCA                                                <  SRR4302535.2989070/94‑1 (MQ=60)
                                               GTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCA                                                >  SRR4302535.4573676/1‑94 (MQ=60)
                                                TGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAG                                               >  SRR4302535.2875689/1‑94 (MQ=60)
                                                 GTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGG                                              <  SRR4302535.2123040/94‑1 (MQ=60)
                                                 GTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGG                                              >  SRR4302535.1687367/1‑94 (MQ=60)
                                                   TGATGGTGTTCTGGGATCGCTTAACCCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGT                                            <  SRR4302535.1641793/94‑1 (MQ=60)
                                                   TGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGT                                            <  SRR4302535.310319/94‑1 (MQ=60)
                                                    GATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTC                                           >  SRR4302535.484425/1‑94 (MQ=60)
                                                    GATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTC                                           >  SRR4302535.1538404/1‑94 (MQ=60)
                                                       GGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAAT                                        >  SRR4302535.2824399/1‑94 (MQ=60)
                                                       GGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAAT                                        <  SRR4302535.2836417/94‑1 (MQ=60)
                                                         TGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTA                                      >  SRR4302535.827180/1‑94 (MQ=60)
                                                          GTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAG                                     <  SRR4302535.506985/94‑1 (MQ=60)
                                                          GTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAG                                     >  SRR4302535.1748044/1‑94 (MQ=60)
                                                          GTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAG                                     <  SRR4302535.2311972/94‑1 (MQ=60)
                                                          GTTCTGGGATCGCTTAAACCGCGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAG                                     <  SRR4302535.139458/94‑1 (MQ=60)
                                                           TTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGC                                    <  SRR4302535.65836/94‑1 (MQ=60)
                                                           TTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGC                                    <  SRR4302535.772945/94‑1 (MQ=60)
                                                             CTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTT                                  <  SRR4302535.4222306/94‑1 (MQ=60)
                                                             CTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTT                                  >  SRR4302535.3172180/1‑94 (MQ=60)
                                                               GGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCG                                >  SRR4302535.2880155/1‑94 (MQ=60)
                                                               GGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCG                                <  SRR4302535.3859389/94‑1 (MQ=60)
                                                               GGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCG                                >  SRR4302535.4144370/1‑94 (MQ=60)
                                                                 GATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTT                              >  SRR4302535.1952956/1‑94 (MQ=60)
                                                                   TCGCTTAAACCGGGCAAACGCGCCAGCCGCGTTGCGCTTGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTT                            <  SRR4302535.2240805/94‑1 (MQ=60)
                                                                   TCGCTAAACCCGGGCAATCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTT                            <  SRR4302535.2423332/94‑1 (MQ=60)
                                                                    CGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTG                           >  SRR4302535.1317107/1‑94 (MQ=60)
                                                                     GCTTAAACGGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCAGGCTACATGTGCAACGAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGA                          >  SRR4302535.1249213/1‑94 (MQ=60)
                                                                      CTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGAT                         <  SRR4302535.4608536/94‑1 (MQ=60)
                                                                      CTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGAT                         <  SRR4302535.4058299/94‑1 (MQ=60)
                                                                       TTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATA                        <  SRR4302535.2610035/94‑1 (MQ=60)
                                                                         AAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGT                      <  SRR4302535.759321/94‑1 (MQ=60)
                                                                         AAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGT                      <  SRR4302535.711826/94‑1 (MQ=60)
                                                                         AAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGT                      >  SRR4302535.3455213/1‑94 (MQ=60)
                                                                         AAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGT                      >  SRR4302535.3309711/1‑94 (MQ=60)
                                                                          AACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTT                     <  SRR4302535.861719/94‑1 (MQ=60)
                                                                             CGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGC                  >  SRR4302535.1933343/1‑94 (MQ=60)
                                                                              GGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCT                 <  SRR4302535.2842009/94‑1 (MQ=60)
                                                                                 CAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCT              >  SRR4302535.2363883/1‑94 (MQ=60)
                                                                                 CAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCT              >  SRR4302535.1357961/1‑94 (MQ=60)
                                                                                 CAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCT              <  SRR4302535.3429588/94‑1 (MQ=60)
                                                                                  AAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTT             <  SRR4302535.1756466/94‑1 (MQ=60)
                                                                                  AAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTT             >  SRR4302535.3203450/1‑94 (MQ=60)
                                                                                   AACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTT            >  SRR4302535.2815045/1‑94 (MQ=60)
                                                                                     CGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTAT          <  SRR4302535.53434/94‑1 (MQ=60)
                                                                                       CGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTG        <  SRR4302535.3385917/94‑1 (MQ=60)
                                                                                        GCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGG       <  SRR4302535.2407385/94‑1 (MQ=60)
                                                                                        GCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGG       >  SRR4302535.2501372/1‑94 (MQ=60)
                                                                                        GCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGG       <  SRR4302535.3935650/94‑1 (MQ=60)
                                                                                        GCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGG       >  SRR4302535.1591759/1‑94 (MQ=60)
                                                                                         CCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGG      <  SRR4302535.1266703/94‑1 (MQ=60)
                                                                                         CCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGG      <  SRR4302535.4302843/94‑1 (MQ=60)
                                                                                         CCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGG      <  SRR4302535.3158296/94‑1 (MQ=60)
                                                                                         CCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGG      <  SRR4302535.756604/94‑1 (MQ=60)
                                                                                         CCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGG      >  SRR4302535.2446677/1‑94 (MQ=60)
                                                                                          CAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGC     <  SRR4302535.629965/94‑1 (MQ=60)
                                                                                           AGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGCT    >  SRR4302535.1621771/1‑94 (MQ=60)
                                                                                             CGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGCCTT  <  SRR4302535.3060809/94‑1 (MQ=60)
                                                                                             CGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGCCTT  >  SRR4302535.3775199/1‑94 (MQ=60)
                                                                                             |                                                                                             
TGCGGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGAGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGCCTT  >  NC_000913/3279104‑3279290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑