Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,305,887	T→C	T227A (ACC→GCC)	yhbM ←	putative control proteins

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,305,887	0	T	C	100.0%	642.6 / NA	207	T227A (ACC→GCC)	yhbM	putative control proteins
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (109/98); total (109/98)

Only 100 of 216 total aligned reads displayed.

ACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGTGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCGACAATGTTCCATCCCCACT  >  NC_000913/3305797‑3305976

aCAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGgcgt                                                                                         <  2:3983010/93‑1 (MQ=255)

aCAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGgcgt                                                                                         >  2:3697044/1‑93 (MQ=255)

  aGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTa                                                                                       <  2:1440255/93‑1 (MQ=255)

  aGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTa                                                                                       <  2:1496676/93‑1 (MQ=255)

  aGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTa                                                                                       <  2:1724028/93‑1 (MQ=255)

   gTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTAt                                                                                      >  2:3384073/1‑93 (MQ=255)

     gCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATcc                                                                                    >  2:3877028/1‑93 (MQ=255)

      ccGTGGCGCTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCg                                                                                   >  2:2351977/1‑93 (MQ=255)

       cGTGGCGCTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGt                                                                                  <  1:98151/93‑1 (MQ=255)

       cGTGGCGCTGTCCAAATCCACCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGt                                                                                  <  1:800900/93‑1 (MQ=255)

        gTGGCGCTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGtt                                                                                 <  1:1379211/93‑1 (MQ=255)

         tGGAGCTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTg                                                                                <  2:2552706/93‑1 (MQ=255)

          ggCGCTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGc                                                                               >  1:4390379/1‑93 (MQ=255)

             gcTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTc                                                                            >  1:937820/1‑93 (MQ=255)

             gcTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTc                                                                            >  2:4151457/1‑93 (MQ=255)

               tGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCg                                                                          >  1:292915/1‑93 (MQ=255)

                 tCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCACAGCGAGCGAGGCGTTATCCGTTGCGTCCGcc                                                                        <  1:1302269/93‑1 (MQ=255)

                     aaTCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGa                                                                    <  1:567934/93‑1 (MQ=255)

                      agcccccAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAg                                                                   <  2:2516662/91‑1 (MQ=255)

                      aTCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGCGCGAGGCGTTATCCGTTGCGTCCGCCTTGAg                                                                   <  1:523098/93‑1 (MQ=255)

                       tCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGc                                                                  <  1:4514495/93‑1 (MQ=255)

                        cccccAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGcc                                                                 <  1:1456930/93‑1 (MQ=255)

                         ccccAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCt                                                                <  1:681664/93‑1 (MQ=255)

                         ccacAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCt                                                                <  1:492018/90‑1 (MQ=255)

                            cAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTc                                                             <  1:2780300/93‑1 (MQ=255)

                            cAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTc                                                             <  1:2979840/93‑1 (MQ=255)

                            cAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTc                                                             >  1:408418/1‑93 (MQ=255)

                             aGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGATCCTTTcc                                                            >  2:541390/1‑93 (MQ=255)

                             aGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTcc                                                            >  1:1759183/1‑93 (MQ=255)

                              gACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCa                                                           >  2:1635015/1‑93 (MQ=255)

                              gACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCa                                                           <  2:1291997/93‑1 (MQ=255)

                              gACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCa                                                           <  1:3926951/93‑1 (MQ=255)

                              gACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCa                                                           <  2:4684430/93‑1 (MQ=255)

                                cTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCAtt                                                         >  1:3026451/1‑93 (MQ=255)

                                cTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCAtt                                                         <  2:665556/93‑1 (MQ=255)

                                 ttAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGGCTTTCCATTa                                                        >  1:3525003/1‑93 (MQ=255)

                                 ttAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTa                                                        >  1:4418410/1‑93 (MQ=255)

                                 ttAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATACGTTGCGTCCGCCTTGAGCCTTTCCATTa                                                        >  1:4276317/1‑93 (MQ=255)

                                  tAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTaa                                                       >  1:3674231/1‑93 (MQ=255)

                                  tAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTaa                                                       <  1:4212638/93‑1 (MQ=255)

                                   aGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAAc                                                      <  2:2781673/93‑1 (MQ=255)

                                    ggTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACg                                                     >  2:2161675/1‑93 (MQ=255)

                                     gtagtaCTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGt                                                    <  2:2020985/93‑1 (MQ=255)

                                      tagtaCTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGtt                                                   <  1:1025727/93‑1 (MQ=255)

                                       agtaCTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGttt                                                  <  2:1047437/93‑1 (MQ=255)

                                       agtaCTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGttt                                                  <  1:4038769/93‑1 (MQ=255)

                                         taCTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGt                                                >  1:1159964/1‑93 (MQ=255)

                                         taCTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGt                                                >  1:3696822/1‑93 (MQ=255)

                                          aCTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGtt                                               >  1:3869423/1‑93 (MQ=255)

                                          aCTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGtt                                               <  2:2424172/93‑1 (MQ=255)

                                            ttACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCg                                             >  2:3019760/1‑93 (MQ=255)

                                            ttACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCg                                             >  2:464437/1‑93 (MQ=255)

                                            ttACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCg                                             >  1:3493384/1‑93 (MQ=255)

                                                cTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTaa                                         <  2:4540421/93‑1 (MQ=255)

                                                 tAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGGGCCTTTCCTTTAACGTTTGTTCGCTAAt                                        >  2:2616231/1‑93 (MQ=255)

                                                 tAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAAt                                        >  1:1737055/1‑93 (MQ=255)

                                                 tAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAAt                                        >  1:4047687/1‑93 (MQ=255)

                                                 tAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAAt                                        <  1:3712273/93‑1 (MQ=255)

                                                   aaTAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGt                                      <  1:2572002/93‑1 (MQ=255)

                                                   aaTAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGt                                      <  2:1933490/93‑1 (MQ=255)

                                                   aaTAGAAGTGGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGt                                      <  2:3783141/93‑1 (MQ=255)

                                                    aTAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGtt                                     <  2:3000595/93‑1 (MQ=255)

                                                    aTAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGtt                                     <  1:220187/93‑1 (MQ=255)

                                                     tAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTg                                    >  1:181942/1‑93 (MQ=255)

                                                      aGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGc                                   >  2:434885/1‑93 (MQ=255)

                                                      aGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGc                                   <  2:3311490/93‑1 (MQ=255)

                                                      aGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGc                                   >  1:3768099/1‑93 (MQ=255)

                                                       gAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGcc                                  >  2:2069165/1‑93 (MQ=255)

                                                       gAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGcc                                  >  2:3219380/1‑93 (MQ=255)

                                                        aaGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGccc                                 <  1:763987/93‑1 (MQ=255)

                                                         aGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTNCANTAACGTTTGTTCGCTAANGTTGCCCa                                >  2:3778864/1‑93 (MQ=255)

                                                         aGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCa                                <  2:1653759/93‑1 (MQ=255)

                                                         aGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCa                                <  1:1407838/93‑1 (MQ=255)

                                                           ttGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAgg                              >  2:2117563/1‑93 (MQ=255)

                                                           ttGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGATAATGTTGCCCAgg                              >  1:1642040/1‑93 (MQ=255)

                                                            tGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGt                             <  1:2551158/93‑1 (MQ=255)

                                                            tGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGt                             >  1:1583712/1‑93 (MQ=255)

                                                              gtatCACTGACAGGATCAGCGGGCGAGGCGTTATCCGTTGCGTCCGCCTAGATCATTTCCATAGACGTTTGTTCGCCAATGTTGCCCAGGTAg                           <  2:4094772/90‑1 (MQ=255)

                                                               tttCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTCACGTTTGTTCGCTAATGTTGCCCAGGCAGa                          >  1:792372/1‑93 (MQ=255)

                                                                ttCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGaa                         >  2:1241670/1‑93 (MQ=255)

                                                                   aCTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTc                      >  1:2910334/1‑93 (MQ=255)

                                                                    cTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCg                     >  1:15437/1‑93 (MQ=255)

                                                                    cTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCg                     >  2:1334450/1‑93 (MQ=255)

                                                                     tGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCGa                    >  2:3751290/1‑93 (MQ=255)

                                                                     tGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCGa                    >  2:1386268/1‑93 (MQ=255)

                                                                       agaTGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCGACa                  <  2:3290417/93‑1 (MQ=255)

                                                                        gaTGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCGACaa                 <  1:322047/93‑1 (MQ=255)

                                                                             tCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCGACAATGTTc            >  2:4251369/1‑93 (MQ=255)

                                                                              cAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCGACAATGTTcc           <  2:718392/93‑1 (MQ=255)

                                                                               agcgagcgAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCGACAATGTTCCa          <  2:3637978/93‑1 (MQ=255)

                                                                               agcgagcgAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCGACAATGTTCCa          <  1:3861587/93‑1 (MQ=255)

                                                                                gcgagcgaGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCGACAATGTTCCAt         <  1:2668485/93‑1 (MQ=255)

                                                                                  gagcgagGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCGACAATGTTCCATcc       >  2:1128481/1‑93 (MQ=255)

                                                                                   agcgagGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCCTTAACGTTTGTTCGCTAATGTTGCCCAGGGAGAACTCGACAATGTTCCATccc      >  2:4377924/1‑93 (MQ=255)

                                                                                    gcgagGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCGACAATGTTCCATcccc     >  1:190926/1‑93 (MQ=255)

                                                                                    gcgagGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCGACAATGTTCCATcccc     <  1:2267540/93‑1 (MQ=255)

                                                                                      gagGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCGACAATGTTCCATCCCCAc   <  2:3446148/93‑1 (MQ=255)

                                                                                      gagGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCGACAATGTTCCATCCCCAc   <  1:2095470/93‑1 (MQ=255)

                                                                                      gagGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCGACAATGTTCCATCCCCAc   <  1:3660052/93‑1 (MQ=255)

                                                                                       agGCGTTATCCGTTGCGTCCGCCTTGATCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCGACAATGTTCCATCCCctct  <  2:2726893/93‑1 (MQ=255)

ACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGTGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCGACAATGTTCCATCCCCACT  >  NC_000913/3305797‑3305976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGTGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCGACAATGTTCCATCCCCACTGTT  >  NC_000913/3305794‑3305979

TGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGC                                                                                              >  SRR4302535.3528986/1‑94 (MQ=60)

 GAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCG                                                                                             <  SRR4302535.2162709/94‑1 (MQ=60)

  AACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGT                                                                                            >  SRR4302535.2236329/1‑94 (MQ=60)

     AGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTAT                                                                                         >  SRR4302535.2586809/1‑94 (MQ=60)

     AGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTAT                                                                                         >  SRR4302535.355484/1‑94 (MQ=60)

       TGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCC                                                                                       >  SRR4302535.1824315/1‑94 (MQ=60)

       TGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCC                                                                                       >  SRR4302535.2950795/1‑94 (MQ=60)

          CGTGGCGCTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTT                                                                                    <  SRR4302535.98845/94‑1 (MQ=60)

          CGTGGCGCTGTCCAAATCCACCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTT                                                                                    <  SRR4302535.806528/94‑1 (MQ=60)

           GTGGCGCTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTG                                                                                   >  SRR4302535.1281780/1‑94 (MQ=60)

           GTGGCGCTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTG                                                                                   <  SRR4302535.1389047/94‑1 (MQ=60)

           GTGGCGCTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTG                                                                                   <  SRR4302535.1531521/94‑1 (MQ=60)

            TGGCGCTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTATATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGC                                                                                  <  SRR4302535.1109678/94‑1 (MQ=60)

            TGGCGCTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGC                                                                                  >  SRR4302535.4420418/1‑94 (MQ=60)

               CGCTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTC                                                                               >  SRR4302535.944463/1‑94 (MQ=60)

                 CTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCG                                                                             >  SRR4302535.294963/1‑94 (MQ=60)

                 CTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCG                                                                             <  SRR4302535.4071656/94‑1 (MQ=60)

                    TCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCT                                                                          <  SRR4302535.1555014/94‑1 (MQ=60)

                    TCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCACAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCT                                                                          <  SRR4302535.1311570/94‑1 (MQ=60)

                        AATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAG                                                                      <  SRR4302535.571919/94‑1 (MQ=60)

                         ATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGCGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGC                                                                     <  SRR4302535.526757/94‑1 (MQ=60)

                          TCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCC                                                                    <  SRR4302535.4545451/94‑1 (MQ=60)

                           CCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCT                                                                   <  SRR4302535.1467356/94‑1 (MQ=60)

                           CCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCT                                                                   >  SRR4302535.949505/1‑94 (MQ=60)

                            CCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTT                                                                  <  SRR4302535.686437/94‑1 (MQ=60)

                            CCACAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTT                                                                  <  SRR4302535.495466/94‑1 (MQ=60)

                              CCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTC                                                                >  SRR4302535.411282/1‑94 (MQ=60)

                              CCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTC                                                                <  SRR4302535.4609977/94‑1 (MQ=60)

                               CAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCC                                                               <  SRR4302535.3000748/94‑1 (MQ=60)

                               CAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCC                                                               <  SRR4302535.2800094/94‑1 (MQ=60)

                               CAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCC                                                               >  SRR4302535.1771922/1‑94 (MQ=60)

                               CAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCC                                                               >  SRR4302535.3448433/1‑94 (MQ=60)

                                 GACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCAT                                                             <  SRR4302535.3953778/94‑1 (MQ=60)

                                  ACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATT                                                            <  SRR4302535.439240/94‑1 (MQ=60)

                                  ACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATT                                                            >  SRR4302535.3047640/1‑94 (MQ=60)

                                   CTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGGCTTTCCATTA                                                           >  SRR4302535.3549482/1‑94 (MQ=60)

                                   CTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTA                                                           >  SRR4302535.4448663/1‑94 (MQ=60)

                                   CTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATACGTTGCGTCCGCCTTGAGCCTTTCCATTA                                                           >  SRR4302535.4305587/1‑94 (MQ=60)

                                    TTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAA                                                          >  SRR4302535.3699672/1‑94 (MQ=60)

                                     TAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAAC                                                         <  SRR4302535.3688175/94‑1 (MQ=60)

                                     TAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAAC                                                         <  SRR4302535.4241471/94‑1 (MQ=60)

                                      AGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACG                                                        >  SRR4302535.3830789/1‑94 (MQ=60)

                                       GGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGT                                                       <  SRR4302535.3179011/94‑1 (MQ=60)

                                        GTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTT                                                      >  SRR4302535.617889/1‑94 (MQ=60)

                                         TAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTT                                                     <  SRR4302535.3526106/94‑1 (MQ=60)

                                         TAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTT                                                     <  SRR4302535.1033013/94‑1 (MQ=60)

                                         TAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTT                                                     <  SRR4302535.214533/94‑1 (MQ=60)

                                         TAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTT                                                     <  SRR4302535.4101876/94‑1 (MQ=60)

                                          AGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTG                                                    <  SRR4302535.2718946/94‑1 (MQ=60)

                                          AGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTG                                                    <  SRR4302535.4066416/94‑1 (MQ=60)

                                          AGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTG                                                    >  SRR4302535.1926733/1‑94 (MQ=60)

                                           GTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCTTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGT                                                   <  SRR4302535.3284947/94‑1 (MQ=60)

                                           GTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGT                                                   >  SRR4302535.3722405/1‑94 (MQ=60)

                                           GTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGT                                                   >  SRR4302535.1168257/1‑94 (MQ=60)

                                            TACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTT                                                  >  SRR4302535.3896018/1‑94 (MQ=60)

                                            TACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTT                                                  >  SRR4302535.3929441/1‑94 (MQ=60)

                                            TACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTT                                                  >  SRR4302535.2362105/1‑94 (MQ=60)

                                              CTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCG                                                >  SRR4302535.847759/1‑94 (MQ=60)

                                              CTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCG                                                >  SRR4302535.3517662/1‑94 (MQ=60)

                                                TACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCT                                              >  SRR4302535.4263003/1‑94 (MQ=60)

                                                   CTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAAT                                           >  SRR4302535.4075394/1‑94 (MQ=60)

                                                   CTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAAT                                           >  SRR4302535.1749610/1‑94 (MQ=60)

                                                   CTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAAT                                           >  SRR4302535.2614101/1‑94 (MQ=60)

                                                    TAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATG                                          >  SRR4302535.1590975/1‑94 (MQ=60)

                                                    TAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATG                                          <  SRR4302535.3737990/94‑1 (MQ=60)

                                                     AAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGT                                         <  SRR4302535.140373/94‑1 (MQ=60)

                                                      AATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTT                                        <  SRR4302535.2590488/94‑1 (MQ=60)

                                                      AATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTT                                        >  SRR4302535.1879083/1‑94 (MQ=60)

                                                      AATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTT                                        >  SRR4302535.3714748/1‑94 (MQ=60)

                                                       ATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTG                                       <  SRR4302535.221703/94‑1 (MQ=60)

                                                       ATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTG                                       <  SRR4302535.4235543/94‑1 (MQ=60)

                                                       ATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTG                                       >  SRR4302535.183201/1‑94 (MQ=60)

                                                       ATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTG                                       <  SRR4302535.1377702/94‑1 (MQ=60)

                                                        TAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGC                                      >  SRR4302535.3794113/1‑94 (MQ=60)

                                                         AGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCC                                     >  SRR4302535.1623184/1‑94 (MQ=60)

                                                          GAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCC                                    >  SRR4302535.1428560/1‑94 (MQ=60)

                                                           AAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCA                                   <  SRR4302535.769369/94‑1 (MQ=60)

                                                           AAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCA                                   >  SRR4302535.3890217/1‑94 (MQ=60)

                                                           AAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCA                                   <  SRR4302535.2802686/94‑1 (MQ=60)

                                                            AGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAG                                  <  SRR4302535.1417896/94‑1 (MQ=60)

                                                            AGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAG                                  >  SRR4302535.4116644/1‑94 (MQ=60)

                                                             GTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGG                                 <  SRR4302535.506957/94‑1 (MQ=60)

                                                             GTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGG                                 >  SRR4302535.3728374/1‑94 (MQ=60)

                                                             GTTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGATAATGTTGCCCAGG                                 >  SRR4302535.1653912/1‑94 (MQ=60)

                                                              TTGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGT                                >  SRR4302535.1595141/1‑94 (MQ=60)

                                                               TGGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTA                               <  SRR4302535.2569527/94‑1 (MQ=60)

                                                                GGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAG                              <  SRR4302535.758657/94‑1 (MQ=60)

                                                                GGTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAG                              >  SRR4302535.779318/1‑94 (MQ=60)

                                                                 GTTTCACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTCACGTTTGTTCGCTAATGTTGCCCAGGCAGA                             >  SRR4302535.797941/1‑94 (MQ=60)

                                                                     CACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTC                         >  SRR4302535.3881945/1‑94 (MQ=60)

                                                                     CACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTC                         >  SRR4302535.2930894/1‑94 (MQ=60)

                                                                     CACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTC                         >  SRR4302535.989622/1‑94 (MQ=60)

                                                                      ACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCG                        >  SRR4302535.15552/1‑94 (MQ=60)

                                                                      ACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCG                        >  SRR4302535.2745747/1‑94 (MQ=60)

                                                                      ACTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCG                        <  SRR4302535.523828/94‑1 (MQ=60)

                                                                       CTGAGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCGA                       >  SRR4302535.2072512/1‑94 (MQ=60)

                                                                         GAGATGCGCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCGACA                     <  SRR4302535.375024/94‑1 (MQ=60)

                                                                          AGATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCGACAA                    <  SRR4302535.561156/94‑1 (MQ=60)

                                                                           GATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCGACAAT                   <  SRR4302535.324277/94‑1 (MQ=60)

                                                                           GATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCGACAAT                   <  SRR4302535.80427/94‑1 (MQ=60)

                                                                           GATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCGACAAT                   <  SRR4302535.861197/94‑1 (MQ=60)

                                                                            ATGCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCGACAATG                  <  SRR4302535.4439343/94‑1 (MQ=60)

                                                                              GCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCGACAATGTT                <  SRR4302535.1698385/94‑1 (MQ=60)

                                                                              GCTCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCGACAATGTT                >  SRR4302535.3969084/1‑94 (MQ=60)

                                                                                TCAGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCGACAATGTTCC              <  SRR4302535.3015732/94‑1 (MQ=60)

                                                                                  AGCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCGACAATGTTCCAT            <  SRR4302535.3888158/94‑1 (MQ=60)

                                                                                   GCGAGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCGACAATGTTCCATC           <  SRR4302535.2687586/94‑1 (MQ=60)

                                                                                      AGCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCGACAATGTTCCATCCCC        >  SRR4302535.192247/1‑94 (MQ=60)

                                                                                       GCGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCGACAATGTTCCATCCCCA       <  SRR4302535.2283705/94‑1 (MQ=60)

                                                                                        CGAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCGACAATGTTCCATCCCCAC      <  SRR4302535.2939240/94‑1 (MQ=60)

                                                                                         GAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCGACAATGTTCCATCCCCACT     <  SRR4302535.1766786/94‑1 (MQ=60)

                                                                                         GAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCGACAATGTTCCATCCCCACT     <  SRR4302535.2110584/94‑1 (MQ=60)

                                                                                         GAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCGACAATGTTCCATCCCCACT     <  SRR4302535.3685387/94‑1 (MQ=60)

                                                                                         GAGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCGACAATGTTCCATCACCACT     >  SRR4302535.4476531/1‑94 (MQ=60)

                                                                                          AGGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCGACAATGTTCCATCCCCACTG    >  SRR4302535.1335846/1‑94 (MQ=60)

                                                                                           GGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCGACAATGTTCCATCCCCACTGT   >  SRR4302535.345517/1‑94 (MQ=60)

                                                                                           GGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCGACAATGTTCCATCCCCACTGT   <  SRR4302535.3664664/94‑1 (MQ=60)

                                                                                           GGCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCGACAATGTTCCATCCCCACTGT   <  SRR4302535.2065045/94‑1 (MQ=60)

                                                                                            GCGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCGACAATGTTCCATCCCCACTGTT  <  SRR4302535.2594070/94‑1 (MQ=60)

TGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGTAGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGCGAGGTGTTATCCGTTGCGTCCGCCTTGAGCCTTTCCATTAACGTTTGTTCGCTAATGTTGCCCAGGTAGAACTCGACAATGTTCCATCCCCACTGTT  >  NC_000913/3305794‑3305979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`