Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,369,263 A→G V316A (GTG→GCG)  b3224 ← sialic acid transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,369,2630AG97.2% 631.1 / 5.7 211V316A (GTG→GCG) b3224sialic acid transporter
Reads supporting (aligned to +/- strand):  ref base A (3/3);  new base G (93/112);  total (96/115)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.66e-01

Only 100 of 216 total aligned reads displayed.

CAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCACATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTAGAGGAAAGCA  >  NC_000913/3369173‑3369352
                                                                                          |                                                                                         
 aaaCGTACGCTTTGCGGGTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCAt                                                                                        >  1:1981189/1‑93 (MQ=255)
   aCGTACGCTTTGCGGGTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTg                                                                                      <  2:2024143/93‑1 (MQ=255)
   aCGTACGCTTTGCGGGTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTg                                                                                      >  1:3985458/1‑93 (MQ=255)
      tACGCTTTGCGGGTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCt                                                                                   <  1:3881899/93‑1 (MQ=255)
       aCGCTTTGCGGGTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTa                                                                                  <  2:814184/93‑1 (MQ=255)
        cGCTTTGCGGGTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTAc                                                                                 <  2:51713/93‑1 (MQ=255)
         gCTTTGCGGGTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTACAGAACAGCGCATTGGCTACa                                                                                >  1:4632720/1‑93 (MQ=255)
          cTTTGCGGGTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAg                                                                               >  1:877881/1‑93 (MQ=255)
          cTTTGCGGGTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAg                                                                               <  1:3250425/93‑1 (MQ=255)
            ttGCGGGTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTa                                                                             <  2:1639012/93‑1 (MQ=255)
            ttGCGCGTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTa                                                                             <  2:2328502/93‑1 (MQ=255)
              gCGGTTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATg                                                                           <  1:114883/93‑1 (MQ=255)
              gCGGGTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATg                                                                           <  1:961269/93‑1 (MQ=255)
                 ggTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCgg                                                                        >  2:2544387/1‑93 (MQ=255)
                 ggTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCgg                                                                        <  1:48150/93‑1 (MQ=255)
                  gTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCggg                                                                       >  1:1613564/1‑93 (MQ=255)
                  gTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCggg                                                                       <  1:2760542/93‑1 (MQ=255)
                     cccAGCCAGTCACCGAGGACGCCACCTCCGCAGCATCCCACCGCCGCGCCACAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTta                                                                    <  2:1725615/93‑1 (MQ=255)
                     cccAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTta                                                                    >  2:2417054/1‑93 (MQ=255)
                      ccagccagTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCACATTGGCTACAGTATGCGGGTtat                                                                   >  2:4150153/1‑93 (MQ=255)
                       cagccagTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTtata                                                                  <  2:3719680/93‑1 (MQ=255)
                        atccagTCACCGACGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATaa                                                                 <  2:2289577/91‑1 (MQ=255)
                        agccagTCCCCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATaa                                                                 <  1:1019776/93‑1 (MQ=255)
                        agccagTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATaa                                                                 <  1:2114637/93‑1 (MQ=255)
                         gccagTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCACATTGGCTACAGTATGCGGGTTATAAg                                                                >  1:3165080/1‑93 (MQ=255)
                          ccagTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGc                                                               >  2:3777703/1‑93 (MQ=255)
                           cagTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGcc                                                              >  1:2023974/1‑93 (MQ=255)
                             gTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAg                                                            >  1:665645/1‑93 (MQ=255)
                             gTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAg                                                            <  2:2124164/93‑1 (MQ=255)
                              tCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGa                                                           >  1:2881048/1‑93 (MQ=255)
                                aCCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATc                                                         >  1:4510357/1‑93 (MQ=255)
                                 ccGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCg                                                        >  1:3024254/1‑93 (MQ=255)
                                  cGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCgg                                                       >  1:3270132/1‑93 (MQ=255)
                                   gAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGt                                                      <  1:3788689/93‑1 (MQ=255)
                                    aGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGtt                                                     <  2:3416062/93‑1 (MQ=255)
                                    aGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGtt                                                     >  1:1685234/1‑93 (MQ=255)
                                     ggAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGttt                                                    <  2:2911039/93‑1 (MQ=255)
                                           cACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGAt                                              <  2:4628113/93‑1 (MQ=255)
                                            aCCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATa                                             <  1:4365685/93‑1 (MQ=255)
                                            aCCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATa                                             >  1:4189991/1‑93 (MQ=255)
                                            aCCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATa                                             <  1:2642563/93‑1 (MQ=255)
                                             ccTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATAc                                            <  1:3602067/93‑1 (MQ=255)
                                              cTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACg                                           <  1:3030985/93‑1 (MQ=255)
                                               tACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGt                                          <  1:1851339/93‑1 (MQ=255)
                                                aCGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGtt                                         <  1:4336122/93‑1 (MQ=255)
                                                aCGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGtt                                         <  1:1123006/93‑1 (MQ=255)
                                                aCGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGtt                                         <  2:4363923/93‑1 (MQ=255)
                                                aCGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGtt                                         <  1:815759/93‑1 (MQ=255)
                                                 cGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTg                                        <  1:749271/93‑1 (MQ=255)
                                                 cGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTg                                        >  2:1903418/1‑93 (MQ=255)
                                                  gcagcaTCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTgg                                       >  2:4051398/1‑93 (MQ=255)
                                                   cagcaTCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGgc                                      <  1:3687387/93‑1 (MQ=255)
                                                   cagcaTCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGgc                                      <  1:1527657/93‑1 (MQ=255)
                                                     gcaTCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGgcag                                    >  2:3177150/1‑93 (MQ=255)
                                                      caTCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGgcagc                                   <  2:1241361/93‑1 (MQ=255)
                                                       aTCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGgcagca                                  >  2:3279380/1‑93 (MQ=255)
                                                       aTCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGgcagca                                  >  1:2499579/1‑93 (MQ=255)
                                                        tCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGcagcag                                 >  2:3020110/1‑93 (MQ=255)
                                                         cccACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCagcagc                                <  1:3820578/93‑1 (MQ=255)
                                                          ccACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAgcg                               >  1:886215/1‑93 (MQ=255)
                                                           cACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAgcgc                              <  2:4401251/93‑1 (MQ=255)
                                                           cACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAgcgc                              <  2:2234686/93‑1 (MQ=255)
                                                            aCCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGcc                             <  1:1708910/93‑1 (MQ=255)
                                                             ccgccgCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCt                            >  2:3571230/1‑93 (MQ=255)
                                                                 cgcgCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAAt                        <  1:2537657/93‑1 (MQ=255)
                                                                 cgcgCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAAt                        <  1:604283/93‑1 (MQ=255)
                                                                 cgcgCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAAt                        <  2:2484278/93‑1 (MQ=255)
                                                                   cgcCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCg                      <  2:116591/93‑1 (MQ=255)
                                                                    gcCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCgg                     >  1:3203537/1‑93 (MQ=255)
                                                                    gcCAAAGCCACTAAAGAACAGCACATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCgg                     >  2:3651913/1‑93 (MQ=255)
                                                                     ccAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGc                    >  2:2161118/1‑93 (MQ=255)
                                                                       aaaGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCa                  >  2:1484058/1‑93 (MQ=255)
                                                                       aaaGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCa                  <  2:2455933/93‑1 (MQ=255)
                                                                        aaGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCAt                 >  1:4473267/1‑93 (MQ=255)
                                                                          gCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGa               <  2:4235866/93‑1 (MQ=255)
                                                                          gCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGCTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGa               >  2:435355/1‑93 (MQ=255)
                                                                           ccACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAg              >  1:1072497/1‑93 (MQ=255)
                                                                           ccACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAg              <  2:2700939/93‑1 (MQ=255)
                                                                            cACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGt             <  2:331381/93‑1 (MQ=255)
                                                                             aCTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTa            >  1:2186226/1‑93 (MQ=255)
                                                                              cTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTGAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTag           >  2:1625393/1‑93 (MQ=255)
                                                                              cTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTag           >  2:860710/1‑93 (MQ=255)
                                                                              cTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAACCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTag           <  1:999753/93‑1 (MQ=255)
                                                                               tAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTaga          >  2:2192043/1‑93 (MQ=255)
                                                                               tAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTaga          <  1:1178721/93‑1 (MQ=255)
                                                                               tAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTaga          >  1:2274189/1‑93 (MQ=255)
                                                                                aaaGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTagag         <  1:2788486/93‑1 (MQ=255)
                                                                                 aaGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTAGAgg        >  1:1401742/1‑93 (MQ=255)
                                                                                  aGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTAGAGGa       <  1:2324368/93‑1 (MQ=255)
                                                                                  aGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTAGAGGa       >  2:3841621/1‑93 (MQ=255)
                                                                                  aGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTAGAGGa       <  2:582060/93‑1 (MQ=255)
                                                                                  aGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTAGAGGa       >  2:707989/1‑93 (MQ=255)
                                                                                  aGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTAGAGGa       >  2:1438660/1‑93 (MQ=255)
                                                                                   gAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTAGAGGaa      <  2:902504/93‑1 (MQ=255)
                                                                                    aaCAGCGCATTGGCTACAGTATGCGTGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTAGAGGaaa     <  1:439277/93‑1 (MQ=255)
                                                                                    aaCAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTAGAGGaaa     >  2:3958559/1‑93 (MQ=255)
                                                                                    aaCAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTAGAGGaaa     >  1:4263631/1‑93 (MQ=255)
                                                                                     aCAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTAGAGGAAAg    >  1:1418427/1‑93 (MQ=255)
                                                                                      cAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTAGAGGAAAGc   <  1:1389354/93‑1 (MQ=255)
                                                                                       aGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTAGAGGAAAGCa  <  1:3097094/93‑1 (MQ=255)
                                                                                          |                                                                                         
CAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCACATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTAGAGGAAAGCA  >  NC_000913/3369173‑3369352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCACATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTAGAGGAAAGCAAACA  >  NC_000913/3369170‑3369356
                                                                                             |                                                                                             
CTACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCG                                                                                               <  SRR4302535.1218292/94‑1 (MQ=60)
 TACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGC                                                                                              <  SRR4302535.1092325/94‑1 (MQ=60)
  ACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCA                                                                                             <  SRR4302535.3762222/94‑1 (MQ=60)
   CAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCAT                                                                                            >  SRR4302535.1995454/1‑94 (MQ=60)
     AACGTACGCTTTGCGGGTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTG                                                                                          <  SRR4302535.2319110/94‑1 (MQ=60)
     AACGTACGCTTTGCGGGTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTG                                                                                          >  SRR4302535.4012695/1‑94 (MQ=60)
      ACGTACGCTTTGCGGGTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGG                                                                                         <  SRR4302535.2462682/94‑1 (MQ=60)
      ACGTACGCTTTGCGGGTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCACATTGG                                                                                         <  SRR4302535.3961907/94‑1 (MQ=60)
       CGTACGCTTTGCGGGTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGC                                                                                        <  SRR4302535.3029856/94‑1 (MQ=60)
        GTACGCTTTGCGGGTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCGTCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCT                                                                                       <  SRR4302535.2526766/94‑1 (MQ=60)
        GTACGCTTTGCGGGTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCT                                                                                       <  SRR4302535.1330101/94‑1 (MQ=60)
         TACGCTTTGCGGGTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTA                                                                                      <  SRR4302535.3908536/94‑1 (MQ=60)
          ACGCTTTGCGGGTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTAC                                                                                     >  SRR4302535.2015963/1‑94 (MQ=60)
           CGCTTTGCGGGTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTACAGAACAGCGCATTGGCTACA                                                                                    >  SRR4302535.4664151/1‑94 (MQ=60)
           CGCTTTGCGGGTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACA                                                                                    >  SRR4302535.1507996/1‑94 (MQ=60)
            GCTTTGCGGGTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAG                                                                                   <  SRR4302535.3381214/94‑1 (MQ=60)
            GCTTTGCGGGTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAG                                                                                   >  SRR4302535.884097/1‑94 (MQ=60)
             CTTTGCGGGTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGT                                                                                  <  SRR4302535.3273008/94‑1 (MQ=60)
               TTGCGGGTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTAT                                                                                <  SRR4302535.325157/94‑1 (MQ=60)
               TTGCGGGTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTAT                                                                                <  SRR4302535.1155969/94‑1 (MQ=60)
                 GCGGTTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGC                                                                              <  SRR4302535.115689/94‑1 (MQ=60)
                 GCGGGTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCCCCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGC                                                                              <  SRR4302535.1079761/94‑1 (MQ=60)
                 GCGGGTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGC                                                                              <  SRR4302535.968098/94‑1 (MQ=60)
                  CGGGTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCG                                                                             <  SRR4302535.3117218/94‑1 (MQ=60)
                    GGTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGG                                                                           >  SRR4302535.1625194/1‑94 (MQ=60)
                    GGTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGG                                                                           <  SRR4302535.48511/94‑1 (MQ=60)
                     GTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGT                                                                          <  SRR4302535.2916769/94‑1 (MQ=60)
                     GTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGT                                                                          <  SRR4302535.2780215/94‑1 (MQ=60)
                     GTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGT                                                                          <  SRR4302535.2107617/94‑1 (MQ=60)
                      TTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTT                                                                         >  SRR4302535.887404/1‑94 (MQ=60)
                         CCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATA                                                                      >  SRR4302535.1932928/1‑94 (MQ=60)
                           AGCCAGTCCCCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAG                                                                    <  SRR4302535.1027020/94‑1 (MQ=60)
                           AGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAG                                                                    <  SRR4302535.972676/94‑1 (MQ=60)
                           AGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAG                                                                    <  SRR4302535.2129899/94‑1 (MQ=60)
                           AGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCACATTGGCTACAGTATGCGGGTTATAAG                                                                    >  SRR4302535.3187022/1‑94 (MQ=60)
                            GCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGC                                                                   <  SRR4302535.3838772/94‑1 (MQ=60)
                             CCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCC                                                                  >  SRR4302535.2038561/1‑94 (MQ=60)
                             CCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCC                                                                  <  SRR4302535.1640778/94‑1 (MQ=60)
                              CAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCA                                                                 >  SRR4302535.2681678/1‑94 (MQ=60)
                              CAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCA                                                                 <  SRR4302535.4362964/94‑1 (MQ=60)
                              CAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCA                                                                 >  SRR4302535.2350327/1‑94 (MQ=60)
                               AGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAG                                                                >  SRR4302535.670289/1‑94 (MQ=60)
                               AGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAG                                                                >  SRR4302535.1423546/1‑94 (MQ=60)
                               AGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCACATTGGCTACAGTATGCGGGTTATAAGCCAG                                                                <  SRR4302535.1775328/94‑1 (MQ=60)
                                GTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGA                                                               >  SRR4302535.2901440/1‑94 (MQ=60)
                                  CACCGAGTAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATC                                                             <  SRR4302535.3304960/94‑1 (MQ=60)
                                  CACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATC                                                             >  SRR4302535.4541299/1‑94 (MQ=60)
                                  CACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATC                                                             <  SRR4302535.823857/94‑1 (MQ=60)
                                   ACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCG                                                            >  SRR4302535.3045426/1‑94 (MQ=60)
                                   ACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCG                                                            <  SRR4302535.1614326/94‑1 (MQ=60)
                                   ACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCG                                                            >  SRR4302535.815509/1‑94 (MQ=60)
                                    CCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGG                                                           >  SRR4302535.3292848/1‑94 (MQ=60)
                                      GAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTT                                                         <  SRR4302535.1425332/94‑1 (MQ=60)
                                      GAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTT                                                         <  SRR4302535.3814820/94‑1 (MQ=60)
                                      GAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTT                                                         >  SRR4302535.1697438/1‑94 (MQ=60)
                                      GAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTT                                                         <  SRR4302535.1171533/94‑1 (MQ=60)
                                        GGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTT                                                       <  SRR4302535.1404720/94‑1 (MQ=60)
                                        GGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTT                                                       >  SRR4302535.3049506/1‑94 (MQ=60)
                                         GAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTC                                                      >  SRR4302535.807460/1‑94 (MQ=60)
                                         GAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTC                                                      <  SRR4302535.664423/94‑1 (MQ=60)
                                              CACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTTAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATA                                                 >  SRR4302535.4129626/1‑94 (MQ=60)
                                              CACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATA                                                 >  SRR4302535.4218668/1‑94 (MQ=60)
                                               ACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATAC                                                <  SRR4302535.2661488/94‑1 (MQ=60)
                                               ACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATAC                                                <  SRR4302535.4395558/94‑1 (MQ=60)
                                                CCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACG                                               <  SRR4302535.3627064/94‑1 (MQ=60)
                                                 CTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGT                                              <  SRR4302535.3052205/94‑1 (MQ=60)
                                                 CTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGT                                              <  SRR4302535.4412551/94‑1 (MQ=60)
                                                 CTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGT                                              <  SRR4302535.468758/94‑1 (MQ=60)
                                                  TACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTT                                             <  SRR4302535.1864678/94‑1 (MQ=60)
                                                   ACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTG                                            <  SRR4302535.1131020/94‑1 (MQ=60)
                                                   ACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTG                                            <  SRR4302535.4365778/94‑1 (MQ=60)
                                                   ACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTG                                            <  SRR4302535.821479/94‑1 (MQ=60)
                                                    CGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGG                                           <  SRR4302535.754547/94‑1 (MQ=60)
                                                    CGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGG                                           <  SRR4302535.1127475/94‑1 (MQ=60)
                                                      CAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCA                                         <  SRR4302535.1538662/94‑1 (MQ=60)
                                                      CAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCA                                         <  SRR4302535.3712900/94‑1 (MQ=60)
                                                        GCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGC                                       <  SRR4302535.2167111/94‑1 (MQ=60)
                                                         CATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCA                                      >  SRR4302535.2784700/1‑94 (MQ=60)
                                                         CATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCA                                      >  SRR4302535.2517611/1‑94 (MQ=60)
                                                          ATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAG                                     >  SRR4302535.3282454/1‑94 (MQ=60)
                                                            CCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCG                                   >  SRR4302535.892487/1‑94 (MQ=60)
                                                            CCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCG                                   <  SRR4302535.3846937/94‑1 (MQ=60)
                                                            CCCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCG                                   >  SRR4302535.4577183/1‑94 (MQ=60)
                                                             CCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGC                                  >  SRR4302535.366312/1‑94 (MQ=60)
                                                             CCACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGC                                  <  SRR4302535.2642897/94‑1 (MQ=60)
                                                               ACCGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCT                                <  SRR4302535.1721264/94‑1 (MQ=60)
                                                                 CGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGA                              >  SRR4302535.4505361/1‑94 (MQ=60)
                                                                 CGCCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGA                              >  SRR4302535.3190440/1‑94 (MQ=60)
                                                                   CCGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAAT                            <  SRR4302535.3176794/94‑1 (MQ=60)
                                                                    CGCGCCAGAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATC                           <  SRR4302535.72104/94‑1 (MQ=60)
                                                                    CGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATC                           <  SRR4302535.608512/94‑1 (MQ=60)
                                                                    CGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATC                           <  SRR4302535.4268914/94‑1 (MQ=60)
                                                                    CGCGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATC                           <  SRR4302535.2555937/94‑1 (MQ=60)
                                                                      CGCCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGG                         >  SRR4302535.3225723/1‑94 (MQ=60)
                                                                        CCAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCC                       >  SRR4302535.2277517/1‑94 (MQ=60)
                                                                        CCAAAGCCACTAAAGAACAGCACATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCC                       <  SRR4302535.3886586/94‑1 (MQ=60)
                                                                         CAAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCA                      <  SRR4302535.1920423/94‑1 (MQ=60)
                                                                          AAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCAT                     >  SRR4302535.4503964/1‑94 (MQ=60)
                                                                          AAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCAT                     <  SRR4302535.4199783/94‑1 (MQ=60)
                                                                          AAAGCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCAT                     >  SRR4302535.588044/1‑94 (MQ=60)
                                                                             GCCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAG                  >  SRR4302535.1080120/1‑94 (MQ=60)
                                                                              CCACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGT                 >  SRR4302535.4378387/1‑94 (MQ=60)
                                                                               CACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTA                >  SRR4302535.4452/1‑94 (MQ=60)
                                                                               CACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTA                >  SRR4302535.1721221/1‑94 (MQ=60)
                                                                               CACTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTA                >  SRR4302535.2201804/1‑94 (MQ=60)
                                                                                 CTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTAGA              >  SRR4302535.2290419/1‑94 (MQ=60)
                                                                                 CTAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAACCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTAGA              <  SRR4302535.1006861/94‑1 (MQ=60)
                                                                                  TAAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTAGAG             <  SRR4302535.1187128/94‑1 (MQ=60)
                                                                                   AAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTAGAGG            <  SRR4302535.2808330/94‑1 (MQ=60)
                                                                                   AAAGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTAGAGG            >  SRR4302535.1411747/1‑94 (MQ=60)
                                                                                     AGAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTAGAGGAA          <  SRR4302535.2340999/94‑1 (MQ=60)
                                                                                      GAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTAGAGGAAA         >  SRR4302535.4292828/1‑94 (MQ=60)
                                                                                      GAACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTAGAGGAAA         <  SRR4302535.4513978/94‑1 (MQ=60)
                                                                                       AACAGCGCATTGGCTACAGTATGCGTGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTAGAGGAAAG        <  SRR4302535.442354/94‑1 (MQ=60)
                                                                                       AACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTAGAGGAAAG        >  SRR4302535.1428554/1‑94 (MQ=60)
                                                                                        ACAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTAGAGGAAAGC       <  SRR4302535.381339/94‑1 (MQ=60)
                                                                                         CAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTAGAGGAAAGCA      <  SRR4302535.1399270/94‑1 (MQ=60)
                                                                                         CAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTAGAGGAAAGCA      <  SRR4302535.2814566/94‑1 (MQ=60)
                                                                                         CAGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTAGAGGAAAGCA      <  SRR4302535.91406/94‑1 (MQ=60)
                                                                                          AGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTAGAGGAAAGCAA     <  SRR4302535.3118659/94‑1 (MQ=60)
                                                                                          AGCGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTAGAGGAAAGCAA     >  SRR4302535.959213/1‑94 (MQ=60)
                                                                                            CGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTAGAGGAAAGCAAAC   <  SRR4302535.801876/94‑1 (MQ=60)
                                                                                            CGCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTAGAGGAAAGCAAAA   >  SRR4302535.4145024/1‑94 (MQ=60)
                                                                                             GCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTAGAGGAAAGCAAACA  <  SRR4302535.3044836/94‑1 (MQ=60)
                                                                                             GCATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTAGAGGAAAGCAAACA  <  SRR4302535.1772720/94‑1 (MQ=60)
                                                                                             |                                                                                             
CTACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCACATTGGCTACAGTATGCGGGTTATAAGCCAGATCGGTTTTCAGATACGTTGGCAGCAGCGCCTGAATCGGCCATGAGTAGAGGAAAGCAAACA  >  NC_000913/3369170‑3369356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑