Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,371,235 T→C intergenic (‑24/+98) nanA ← / ← b3226 N‑acetylneuraminate lyase/putative FADA‑type transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,371,2350TC95.0% 799.5 / 19.6 277intergenic (‑24/+98)nanA/b3226N‑acetylneuraminate lyase/putative FADA‑type transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (10/4);  new base C (131/132);  total (141/136)
Fisher's exact test for biased strand distribution p-value = 1.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 281 total aligned reads displayed.

TCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTA  >  NC_000913/3371144‑3371324
                                                                                           |                                                                                         
 ccAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGTCt                                                                                         <  1:4363607/93‑1 (MQ=255)
   aGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGa                                                                                       <  1:3587015/93‑1 (MQ=255)
   aGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGa                                                                                       >  2:3613229/1‑93 (MQ=255)
   aGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGa                                                                                       <  1:1608776/93‑1 (MQ=255)
    gTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGAt                                                                                      <  1:3230954/93‑1 (MQ=255)
    gTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGAt                                                                                      >  1:1657943/1‑93 (MQ=255)
    gTGCTTGTTGTTCGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGAt                                                                                      <  1:1074369/93‑1 (MQ=255)
    gTGCTTGTTGTTAGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGAt                                                                                      <  1:83319/93‑1 (MQ=255)
     tGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATa                                                                                     <  2:3874996/93‑1 (MQ=255)
     tGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATa                                                                                     >  2:3345379/1‑93 (MQ=255)
      gCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATaa                                                                                    >  2:1689912/1‑93 (MQ=255)
       cTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATaaa                                                                                   <  1:2553534/93‑1 (MQ=255)
        ttgttgttgGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAAc                                                                                  <  1:478847/93‑1 (MQ=255)
        ttgttgttgGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAAc                                                                                  >  2:3801325/1‑93 (MQ=255)
        ttgttgttgGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAAc                                                                                  <  1:2060288/93‑1 (MQ=255)
           ttgttgGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGat                                                                               <  2:4322888/93‑1 (MQ=255)
           ttgttgGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGat                                                                               <  2:1713787/93‑1 (MQ=255)
             gttgGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGatat                                                                             <  1:23059/93‑1 (MQ=255)
             gttgGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGatat                                                                             >  2:65129/1‑93 (MQ=255)
             gttgGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGatat                                                                             <  1:579333/93‑1 (MQ=255)
              ttgGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGAtata                                                                            >  1:4035535/1‑93 (MQ=255)
              ttgGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGAtata                                                                            <  2:3977184/93‑1 (MQ=255)
              ttgGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGAtata                                                                            <  2:2522125/93‑1 (MQ=255)
               tgGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATAc                                                                           <  1:2031873/93‑1 (MQ=255)
                 gTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCt                                                                         <  1:131592/93‑1 (MQ=255)
                  tCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCtt                                                                        <  2:2352739/93‑1 (MQ=255)
                   cAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCttt                                                                       <  2:1994767/93‑1 (MQ=255)
                    aaaaGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTta                                                                      <  2:3041010/93‑1 (MQ=255)
                    aaaaGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTta                                                                      <  1:884039/93‑1 (MQ=255)
                    aaaaGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTta                                                                      <  1:1766034/93‑1 (MQ=255)
                     aaaGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTtat                                                                     >  1:3671741/1‑93 (MQ=255)
                     aaaGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTtat                                                                     >  1:1375786/1‑93 (MQ=255)
                     aaaGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTtat                                                                     >  1:2521575/1‑93 (MQ=255)
                     aaaGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTtat                                                                     >  2:2818584/1‑93 (MQ=255)
                     aaaGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTtat                                                                     >  2:2676438/1‑93 (MQ=255)
                      aaGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTCGCCATAAATACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTtata                                                                    >  1:182878/1‑93 (MQ=255)
                        ggAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATAcc                                                                  <  2:3282845/93‑1 (MQ=255)
                        ggAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATAcc                                                                  <  1:2707388/93‑1 (MQ=255)
                         gAGTCAGGAGTGCGGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCt                                                                 >  1:2214224/1‑93 (MQ=255)
                           gTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGt                                                               >  1:3840913/1‑93 (MQ=255)
                             cAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTta                                                             <  2:3187516/93‑1 (MQ=255)
                               ggAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTtata                                                           >  1:757936/1‑93 (MQ=255)
                               ggAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTtata                                                           <  2:4344539/93‑1 (MQ=255)
                                gAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATAc                                                          <  2:2733038/93‑1 (MQ=255)
                                gAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATAc                                                          >  2:1833697/1‑93 (MQ=255)
                                gAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATAc                                                          <  1:3880857/93‑1 (MQ=255)
                                    gCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGa                                                      <  2:1105939/93‑1 (MQ=255)
                                    gCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGa                                                      <  2:4107684/93‑1 (MQ=255)
                                       gCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCa                                                   <  1:1241434/93‑1 (MQ=255)
                                        ccATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCaa                                                  <  1:3214615/93‑1 (MQ=255)
                                         cATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAAt                                                 >  1:1172084/1‑93 (MQ=255)
                                           ttACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTa                                               <  1:4302157/93‑1 (MQ=255)
                                           ttACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTa                                               <  2:3925938/93‑1 (MQ=255)
                                             aCGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAg                                             >  1:4462979/1‑93 (MQ=255)
                                               gCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCa                                           >  2:1644057/1‑93 (MQ=255)
                                               gCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCa                                           >  1:1720656/1‑93 (MQ=255)
                                                ccACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCaa                                          >  2:2061113/1‑93 (MQ=255)
                                                 cACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAac                                         >  2:1475191/1‑93 (MQ=255)
                                                 cACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAac                                         >  1:4528747/1‑93 (MQ=255)
                                                  aCGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAaca                                        >  2:370592/1‑93 (MQ=255)
                                                  aCGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAaca                                        >  1:2863587/1‑93 (MQ=255)
                                                   cGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAacac                                       >  1:3732102/1‑93 (MQ=255)
                                                   cGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAacac                                       >  1:2355990/1‑93 (MQ=255)
                                                    gTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACAcc                                      >  2:1276664/1‑93 (MQ=255)
                                                    gTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACAcc                                      >  1:4055968/1‑93 (MQ=255)
                                                      aaaTTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACAcccc                                    >  2:2993763/1‑93 (MQ=255)
                                                         ttCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATa                                 >  1:3522958/1‑93 (MQ=255)
                                                          tCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATAc                                <  1:2953287/93‑1 (MQ=255)
                                                          tCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATAc                                <  2:4625404/93‑1 (MQ=255)
                                                          tCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATAc                                <  2:1210793/93‑1 (MQ=255)
                                                           cGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACa                               <  1:3430830/93‑1 (MQ=255)
                                                              tGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGaa                            >  2:862613/1‑93 (MQ=255)
                                                                 cATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGc                         <  1:997136/93‑1 (MQ=255)
                                                                    aaaTACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTta                      <  2:479794/93‑1 (MQ=255)
                                                                     aaTAGCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTtat                     >  2:4539769/1‑93 (MQ=255)
                                                                      aTACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTtata                    <  1:1862469/93‑1 (MQ=255)
                                                                      aTACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTtata                    <  2:3517195/93‑1 (MQ=255)
                                                                      aTACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTtata                    <  2:3555780/93‑1 (MQ=255)
                                                                       tACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATaa                   >  1:2174083/1‑93 (MQ=255)
                                                                         ccTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATg                 >  2:4661477/1‑93 (MQ=255)
                                                                         ccTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATg                 >  2:2911917/1‑93 (MQ=255)
                                                                             tGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGAt             >  2:3151104/1‑93 (MQ=255)
                                                                              gAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATc            >  1:96040/1‑93 (MQ=255)
                                                                              gAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATc            <  1:3311837/93‑1 (MQ=255)
                                                                              gAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATc            <  1:413827/93‑1 (MQ=255)
                                                                              gAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATc            <  2:4020842/93‑1 (MQ=255)
                                                                               aaGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTAAACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCt           >  2:3427583/1‑93 (MQ=255)
                                                                                 gTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGc         >  1:3544383/1‑93 (MQ=255)
                                                                                  tGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCt        >  1:1531335/1‑93 (MQ=255)
                                                                                   gATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCtt       <  1:2760235/93‑1 (MQ=255)
                                                                                    aTGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTc      <  1:3102318/93‑1 (MQ=255)
                                                                                     tGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCa     >  1:418383/1‑93 (MQ=255)
                                                                                     tGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCa     >  2:4468098/1‑93 (MQ=255)
                                                                                     tGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCa     >  2:1608619/1‑93 (MQ=255)
                                                                                     tGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCa     >  2:357751/1‑93 (MQ=255)
                                                                                      gCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCAc    <  2:2416407/93‑1 (MQ=255)
                                                                                        ttGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTa  <  2:4581798/93‑1 (MQ=255)
                                                                                        ttGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTa  >  2:1779344/1‑93 (MQ=255)
                                                                                        ttGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTa  >  2:590822/1‑93 (MQ=255)
                                                                                        ttGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTa  <  2:2611977/93‑1 (MQ=255)
                                                                                           |                                                                                         
TCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTA  >  NC_000913/3371144‑3371324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TATCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGT  >  NC_000913/3371142‑3371328
                                                                                             |                                                                                             
TATCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGC                                                                                               >  SRR4302535.303571/1‑94 (MQ=60)
 TTCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGGCACGTAAATTCGTTGCCATAAATGCCTCTGAAGTGATGCTTGCC                                                                                              <  SRR4302535.3706034/94‑1 (MQ=60)
 ATCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCC                                                                                              >  SRR4302535.826143/1‑94 (MQ=60)
 ATCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCC                                                                                              >  SRR4302535.794150/1‑94 (MQ=60)
 ATCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCC                                                                                              >  SRR4302535.2265247/1‑94 (MQ=60)
  TCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGTCT                                                                                             <  SRR4302535.1741677/94‑1 (MQ=60)
  TCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCT                                                                                             >  SRR4302535.1261977/1‑94 (MQ=60)
  TCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCT                                                                                             <  SRR4302535.4463029/94‑1 (MQ=60)
   CCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGTCTG                                                                                            <  SRR4302535.4393468/94‑1 (MQ=60)
    CAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGA                                                                                           <  SRR4302535.2802861/94‑1 (MQ=60)
     AGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGAT                                                                                          <  SRR4302535.3448748/94‑1 (MQ=60)
     AGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGAT                                                                                          >  SRR4302535.1669931/1‑94 (MQ=60)
     AGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGAT                                                                                          <  SRR4302535.1620378/94‑1 (MQ=60)
     AGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGAT                                                                                          <  SRR4302535.3611895/94‑1 (MQ=60)
      GTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATA                                                                                         <  SRR4302535.3253354/94‑1 (MQ=60)
      GTGCTTGTTGTTCGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATA                                                                                         <  SRR4302535.1082008/94‑1 (MQ=60)
      GTGCTTGTTGTTAGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATA                                                                                         <  SRR4302535.83907/94‑1 (MQ=60)
        GCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAA                                                                                       >  SRR4302535.90027/1‑94 (MQ=60)
         ctgcgtcTTGGTCAAAAGGAGTCAGGAGTGGAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAAC                                                                                      <  SRR4302535.2635001/87‑1 (MQ=60)
         CTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAAC                                                                                      >  SRR4302535.1383325/1‑94 (MQ=60)
         CTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAAC                                                                                      <  SRR4302535.2571919/94‑1 (MQ=60)
         CTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAAC                                                                                      <  SRR4302535.2544859/94‑1 (MQ=60)
         CTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAAC                                                                                      >  SRR4302535.2060774/1‑94 (MQ=60)
         CTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAAC                                                                                      <  SRR4302535.3047477/94‑1 (MQ=60)
          TTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACG                                                                                     <  SRR4302535.2761366/94‑1 (MQ=60)
          TTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACG                                                                                     <  SRR4302535.2075133/94‑1 (MQ=60)
          TTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACG                                                                                     <  SRR4302535.3590731/94‑1 (MQ=60)
          TTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACG                                                                                     <  SRR4302535.1380353/94‑1 (MQ=60)
          TTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACG                                                                                     <  SRR4302535.482206/94‑1 (MQ=60)
           TGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGTCTGATAAACGA                                                                                    >  SRR4302535.3376601/1‑94 (MQ=60)
           TGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGA                                                                                    >  SRR4302535.3553750/1‑94 (MQ=60)
            GTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGAT                                                                                   <  SRR4302535.1760866/94‑1 (MQ=60)
            GTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGAT                                                                                   <  SRR4302535.4614113/94‑1 (MQ=60)
               GTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATA                                                                                >  SRR4302535.4063161/1‑94 (MQ=60)
               GTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATA                                                                                <  SRR4302535.23239/94‑1 (MQ=60)
               GTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATA                                                                                <  SRR4302535.583405/94‑1 (MQ=60)
                 TGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACC                                                                              <  SRR4302535.2046514/94‑1 (MQ=60)
                  GGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCT                                                                             <  SRR4302535.986700/94‑1 (MQ=60)
                   GTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTT                                                                            <  SRR4302535.3886279/94‑1 (MQ=60)
                   GTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTT                                                                            <  SRR4302535.132510/94‑1 (MQ=60)
                   GTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTT                                                                            >  SRR4302535.830786/1‑94 (MQ=60)
                    TCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTT                                                                           <  SRR4302535.3701333/94‑1 (MQ=60)
                      AAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTAT                                                                         >  SRR4302535.974279/1‑94 (MQ=60)
                      AAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTAT                                                                         >  SRR4302535.3718729/1‑94 (MQ=60)
                      AAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTAT                                                                         >  SRR4302535.4347283/1‑94 (MQ=60)
                      AAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTAT                                                                         >  SRR4302535.3697169/1‑94 (MQ=60)
                      AAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTAT                                                                         >  SRR4302535.1385612/1‑94 (MQ=60)
                      AAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTAT                                                                         <  SRR4302535.1778838/94‑1 (MQ=60)
                      AAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTAT                                                                         >  SRR4302535.2539745/1‑94 (MQ=60)
                      AAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTAT                                                                         <  SRR4302535.890294/94‑1 (MQ=60)
                       AAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTCGCCATAAATACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATA                                                                        >  SRR4302535.184139/1‑94 (MQ=60)
                        AAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATAC                                                                       >  SRR4302535.563408/1‑94 (MQ=60)
                        AAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATAC                                                                       >  SRR4302535.3765824/1‑94 (MQ=60)
                          GGAGTCAGGAGTGCGGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCT                                                                     >  SRR4302535.2229992/1‑94 (MQ=60)
                          GGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCT                                                                     <  SRR4302535.2726735/94‑1 (MQ=60)
                          GGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCT                                                                     <  SRR4302535.1226786/94‑1 (MQ=60)
                           GAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTG                                                                    <  SRR4302535.1582464/94‑1 (MQ=60)
                            AGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGT                                                                   >  SRR4302535.3867379/1‑94 (MQ=60)
                             GTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTT                                                                  >  SRR4302535.1173420/1‑94 (MQ=60)
                             GTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTT                                                                  >  SRR4302535.481238/1‑94 (MQ=60)
                             GTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTT                                                                  <  SRR4302535.194085/94‑1 (MQ=60)
                               CAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTAT                                                                >  SRR4302535.1803561/1‑94 (MQ=60)
                                AGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATA                                                               <  SRR4302535.4211314/94‑1 (MQ=60)
                                AGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATA                                                               >  SRR4302535.3183264/1‑94 (MQ=60)
                                AGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATA                                                               >  SRR4302535.763267/1‑94 (MQ=60)
                                 GGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATAC                                                              <  SRR4302535.3568574/94‑1 (MQ=60)
                                  GAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACC                                                             <  SRR4302535.3907492/94‑1 (MQ=60)
                                  GAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACC                                                             <  SRR4302535.1790540/94‑1 (MQ=60)
                                   AGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCA                                                            >  SRR4302535.3901586/1‑94 (MQ=60)
                                    GTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCCG                                                           >  SRR4302535.3640751/1‑94 (MQ=60)
                                    GTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAG                                                           <  SRR4302535.2901555/94‑1 (MQ=60)
                                      GCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGAT                                                         <  SRR4302535.16327/94‑1 (MQ=60)
                                        AGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCA                                                       >  SRR4302535.220061/1‑94 (MQ=60)
                                         GCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAA                                                      <  SRR4302535.1250300/94‑1 (MQ=60)
                                         GCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAA                                                      >  SRR4302535.4145544/1‑94 (MQ=60)
                                          CCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAAT                                                     >  SRR4302535.1180455/1‑94 (MQ=60)
                                          CCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAAT                                                     <  SRR4302535.3236882/94‑1 (MQ=60)
                                           CATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATT                                                    <  SRR4302535.2510028/94‑1 (MQ=60)
                                             TTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAA                                                  <  SRR4302535.4331590/94‑1 (MQ=60)
                                              TACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAG                                                 >  SRR4302535.4493589/1‑94 (MQ=60)
                                                CGCCACGTAAATTCGTTGCCATAAATCCCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTAATCGCTGTTATACCAGATCAATTAAGCA                                               <  SRR4302535.2568081/94‑1 (MQ=60)
                                                CGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCA                                               >  SRR4302535.1733102/1‑94 (MQ=60)
                                                 GCCACGTAAATTCGTTGCCATAAATACCTCTGAACTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAA                                              >  SRR4302535.1863734/1‑94 (MQ=60)
                                                  CCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAAC                                             >  SRR4302535.782057/1‑94 (MQ=60)
                                                  CCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAAC                                             >  SRR4302535.4559758/1‑94 (MQ=60)
                                                   CACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACA                                            >  SRR4302535.2883877/1‑94 (MQ=60)
                                                    ACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACAC                                           >  SRR4302535.3757944/1‑94 (MQ=60)
                                                    ACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACAC                                           >  SRR4302535.2161779/1‑94 (MQ=60)
                                                    ACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACAC                                           >  SRR4302535.2372884/1‑94 (MQ=60)
                                                     CGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACC                                          >  SRR4302535.3497565/1‑94 (MQ=60)
                                                     CGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACC                                          <  SRR4302535.165667/94‑1 (MQ=60)
                                                     CGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACC                                          >  SRR4302535.4083738/1‑94 (MQ=60)
                                                     CGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACC                                          >  SRR4302535.2593357/1‑94 (MQ=60)
                                                      GTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCC                                         >  SRR4302535.2419688/1‑94 (MQ=60)
                                                      GTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCC                                         <  SRR4302535.3977938/94‑1 (MQ=60)
                                                      GTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCC                                         >  SRR4302535.4535060/1‑94 (MQ=60)
                                                       TAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCA                                        >  SRR4302535.1515187/1‑94 (MQ=60)
                                                        AAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCA                                       >  SRR4302535.2363403/1‑94 (MQ=60)
                                                         AATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCAT                                      >  SRR4302535.2597532/1‑94 (MQ=60)
                                                          ATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATA                                     >  SRR4302535.3547424/1‑94 (MQ=60)
                                                          ATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATA                                     >  SRR4302535.4075969/1‑94 (MQ=60)
                                                           TTCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATAC                                    <  SRR4302535.433174/94‑1 (MQ=60)
                                                            TCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATGCCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACA                                   <  SRR4302535.752390/94‑1 (MQ=60)
                                                            TCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACA                                   <  SRR4302535.485626/94‑1 (MQ=60)
                                                            TCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACA                                   <  SRR4302535.2974035/94‑1 (MQ=60)
                                                            TCGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACA                                   <  SRR4302535.1718994/94‑1 (MQ=60)
                                                             CGTTGCCATAAATATCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAG                                  <  SRR4302535.1674022/94‑1 (MQ=60)
                                                             CGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAG                                  <  SRR4302535.4397867/94‑1 (MQ=60)
                                                             CGTTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAG                                  <  SRR4302535.3454675/94‑1 (MQ=60)
                                                               TTGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAA                                >  SRR4302535.4381239/1‑94 (MQ=60)
                                                                TGCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAA                               <  SRR4302535.2343293/94‑1 (MQ=60)
                                                                 GCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAG                              <  SRR4302535.1282215/94‑1 (MQ=60)
                                                                 GCCATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAG                              >  SRR4302535.4265013/1‑94 (MQ=60)
                                                                   CATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCT                            <  SRR4302535.1004215/94‑1 (MQ=60)
                                                                   CATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCT                            >  SRR4302535.4143113/1‑94 (MQ=60)
                                                                    ATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTT                           <  SRR4302535.643990/94‑1 (MQ=60)
                                                                    ATAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTT                           >  SRR4302535.907687/1‑94 (MQ=60)
                                                                     TAAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTA                          >  SRR4302535.2073495/1‑94 (MQ=60)
                                                                      AAATACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTAT                         <  SRR4302535.2008374/94‑1 (MQ=60)
                                                                      AAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTAT                         >  SRR4302535.1206551/1‑94 (MQ=60)
                                                                      AAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTAT                         <  SRR4302535.3230340/94‑1 (MQ=60)
                                                                      AAATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTAT                         >  SRR4302535.2900906/1‑94 (MQ=60)
                                                                       AATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATA                        >  SRR4302535.3242821/1‑94 (MQ=60)
                                                                        ATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAA                       >  SRR4302535.2189633/1‑94 (MQ=60)
                                                                        ATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAA                       <  SRR4302535.1875901/94‑1 (MQ=60)
                                                                        ATACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAA                       <  SRR4302535.2751030/94‑1 (MQ=60)
                                                                          tccttcgcAGTGATGGTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATG                     <  SRR4302535.1885809/86‑1 (MQ=60)
                                                                          ACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATG                     <  SRR4302535.219694/94‑1 (MQ=60)
                                                                          ACCTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATG                     <  SRR4302535.2263322/94‑1 (MQ=60)
                                                                            CTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCG                   >  SRR4302535.1521944/1‑94 (MQ=60)
                                                                            CTCTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCG                   <  SRR4302535.349866/94‑1 (MQ=60)
                                                                              CTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGTT                 <  SRR4302535.4453678/94‑1 (MQ=60)
                                                                              CTGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGAT                 <  SRR4302535.32470/94‑1 (MQ=60)
                                                                               TGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATC                >  SRR4302535.311799/1‑94 (MQ=60)
                                                                               TGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATC                >  SRR4302535.96721/1‑94 (MQ=60)
                                                                               TGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATC                >  SRR4302535.3283765/1‑94 (MQ=60)
                                                                               TGAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATC                >  SRR4302535.3571841/1‑94 (MQ=60)
                                                                                GAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCT               <  SRR4302535.3334882/94‑1 (MQ=60)
                                                                                GAAGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCT               <  SRR4302535.416733/94‑1 (MQ=60)
                                                                                  AGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGC             >  SRR4302535.3568999/1‑94 (MQ=60)
                                                                                  AGTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGC             <  SRR4302535.852071/94‑1 (MQ=60)
                                                                                   GTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCT            >  SRR4302535.1542368/1‑94 (MQ=60)
                                                                                   GTGATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCT            >  SRR4302535.212292/1‑94 (MQ=60)
                                                                                     GATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTC          <  SRR4302535.2779904/94‑1 (MQ=60)
                                                                                     GATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTC          >  SRR4302535.4056174/1‑94 (MQ=60)
                                                                                      ATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCA         <  SRR4302535.3123912/94‑1 (MQ=60)
                                                                                      ATGCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCA         >  SRR4302535.421315/1‑94 (MQ=60)
                                                                                        GCTTGCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACT       <  SRR4302535.4180672/94‑1 (MQ=60)
                                                                                            GCCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAG   <  SRR4302535.4699775/94‑1 (MQ=60)
                                                                                             CCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGT  >  SRR4302535.2865448/1‑94 (MQ=60)
                                                                                             CCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGT  >  SRR4302535.2121466/1‑94 (MQ=60)
                                                                                             |                                                                                             
TATCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATGCTTGTCTGATAAACGATATACCTTTATACCTGTTATACCAGATCAATTAAGCAACACCCCATACAGAAAGCTTATAATGCGATCTGCTTCACTAAAGT  >  NC_000913/3371142‑3371328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑