Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,405,469	T→C	W76R (TGG→CGG)	b3258 →	sodium/pantothenate symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,405,469	0	T	C	99.3%	461.1 / NA	148	W76R (TGG→CGG)	b3258	sodium/pantothenate symporter
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (46/101); minor base G (0/1); total (46/102)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 193 total aligned reads displayed.

ATTGTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTAC  >  NC_000913/3405379‑3405558

aTTGTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGGCAGGGGCGTCTTTTTAATACGGgctgggccgg                                                                                         >  2:3296772/1‑93 (MQ=255)

  tgtgCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGt                                                                                       <  2:249024/93‑1 (MQ=255)

   gtgCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTa                                                                                      <  2:3076548/93‑1 (MQ=255)

   gtgCTGCCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTa                                                                                      <  2:45460/93‑1 (MQ=255)

    tgCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGGGCTGCGTATAAAAACGGGCTGGGCCGGGAAt                                                                                     >  1:3925997/1‑93 (MQ=255)

    tgCTGGCGATGACGCTAACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTAt                                                                                     <  1:4426632/93‑1 (MQ=255)

     gCTGGCGATGACGCTCTCCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTAtt                                                                                    <  2:4245457/93‑1 (MQ=255)

     gCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTAtt                                                                                    <  1:4610859/93‑1 (MQ=255)

      cTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTg                                                                                   >  2:3014904/1‑93 (MQ=255)

       tGGCGATCACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGc                                                                                  <  1:3290342/93‑1 (MQ=255)

        ggCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCt                                                                                 >  1:1967593/1‑93 (MQ=255)

         gCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGTCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTg                                                                                <  2:4305745/93‑1 (MQ=255)

          cGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTgg                                                                               <  2:2519889/93‑1 (MQ=255)

             tGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCga                                                                            <  2:627985/93‑1 (MQ=255)

                   tCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCCGGAGCTGCTTATAAATACGGGCTGGGCCCGGGATTGCTGGCGGTGAtta                                                                      >  2:2410513/1‑92 (MQ=255)

                    cACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCa                                                                     <  2:4390448/93‑1 (MQ=255)

                    cACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCa                                                                     <  1:1140268/93‑1 (MQ=255)

                     aCCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGATGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAg                                                                    <  1:471405/93‑1 (MQ=255)

                         cgACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTc                                                                <  2:1964364/93‑1 (MQ=255)

                          gACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTTTAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTCAGCTTcc                                                               >  1:646506/1‑93 (MQ=255)

                          gACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTcc                                                               <  2:1103908/93‑1 (MQ=255)

                          caCCTATATCAGTGCCAGTTCGTTTAGCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTcc                                                               <  1:3102546/92‑1 (MQ=255)

                           aCCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCt                                                              >  1:2502062/1‑93 (MQ=255)

                            ccTATAACAGTGCCAGTTCGTTTATCGGCGGGCCCGGAGCTGCTTATAAATACGGGCTGGGGCAGGGATTACTGGCGATGATTAAGCTTTCtc                                                             >  2:3903173/1‑92 (MQ=255)

                             cTATATCAGTGCCAGTTCGTTTATCGGCGGGCCCGGAGCTGGTTATAAATATGGGCCGGGTCGGGTGATGATGGCGCGGATTAAGCTTCCTGc                                                            >  2:136972/1‑93 (MQ=255)

                             cTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGc                                                            <  2:44875/93‑1 (MQ=255)

                              tataTCAGTGCTAGTTCGTTTATCGGCGGGCCAGGATCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCa                                                           <  1:297532/93‑1 (MQ=255)

                              tataTCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCa                                                           <  2:1734197/93‑1 (MQ=255)

                              tataTCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTCAGCTTCCTGCa                                                           >  2:2381911/1‑93 (MQ=255)

                                tatCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTCAGCTTCCTGCAGt                                                         >  1:3726050/1‑93 (MQ=255)

                                 atCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTc                                                        <  2:2786379/93‑1 (MQ=255)

                                 atCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTc                                                        <  1:2911268/93‑1 (MQ=255)

                                    aGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTgg                                                     <  1:1643247/93‑1 (MQ=255)

                                     gTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGGCGGGGATTGCTTGCGATGATTCAGCTTCCCGCCGTCTTGc                                                    >  2:879874/1‑93 (MQ=255)

                                     gTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGc                                                    <  1:3206123/93‑1 (MQ=255)

                                      tGCCAGTTCGTTTATCGGCTGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCt                                                   <  2:1891885/93‑1 (MQ=255)

                                      tGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCt                                                   <  1:1182411/93‑1 (MQ=255)

                                      tGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTTGCGATGATTCAGCTTCCTGCAGTCTGGCt                                                   >  1:1890735/1‑93 (MQ=255)

                                       gCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGAtt                                                  <  1:3862116/93‑1 (MQ=255)

                                        ccAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCttt                                                 <  1:3975001/93‑1 (MQ=255)

                                         cAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTc                                                <  2:4285064/93‑1 (MQ=255)

                                          aGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCa                                               <  2:3830498/93‑1 (MQ=255)

                                           gTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCAc                                              <  1:1489254/93‑1 (MQ=255)

                                            ttCGTTTATCGGCGGGCCAGGGGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTCAGCTTCCTGCAGTCTTGCTTTCACt                                             >  2:3680439/1‑93 (MQ=255)

                                             tCGTTTATCGGCGTGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTc                                            <  2:1385793/93‑1 (MQ=255)

                                             tCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTc                                            >  1:2084447/1‑93 (MQ=255)

                                             tCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTc                                            <  2:1158820/93‑1 (MQ=255)

                                                tttATCGGCGGGGCCGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGGTGATTTAGGTTTCTGCAGGCTGGGTTTCACCCggg                                         >  2:1891074/1‑92 (MQ=255)

                                                tttATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTTGGCCGGGGATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGGTTTCACTCGGt                                         >  2:3531587/1‑93 (MQ=255)

                                                tttATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGt                                         >  1:1592092/1‑93 (MQ=255)

                                                 ttATCGGCGGGCCCGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGGGATGATTCAGGTTTCTGTCGGCTGGCTTTCACTCggct                                        >  2:927308/1‑91 (MQ=255)

                                                 ttATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGGCGGGGATTGCTGGCGGTGATTCAGCTTCCTGCAGTCTGGCTTTTACTCggga                                        >  2:3369885/1‑91 (MQ=255)

                                                 ttATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTa                                        <  1:1723639/93‑1 (MQ=255)

                                                  tATCGGCGGGCCAGGGGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGGAt                                       >  2:2459365/1‑93 (MQ=255)

                                                  tATCGGCGGGCCAGGAGGTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTCAGCTTTCTGCAGTCTGGCTTTCACTCGGTAt                                       >  2:4274553/1‑93 (MQ=255)

                                                  tATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTAt                                       <  1:970132/93‑1 (MQ=255)

                                                  tATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTAt                                       <  1:230790/93‑1 (MQ=255)

                                                  tATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTAt                                       >  2:4683158/1‑93 (MQ=255)

                                                  tATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTAt                                       >  1:2638576/1‑93 (MQ=255)

                                                   aTCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCCGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTTGCTTTCACTCGGTAtt                                      >  2:3117956/1‑93 (MQ=255)

                                                     cggcggGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATtct                                    <  1:10736/93‑1 (MQ=255)

                                                      ggcggGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATtctc                                   <  2:2662751/93‑1 (MQ=255)

                                                      ggcggGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATtctc                                   >  1:217403/1‑93 (MQ=255)

                                                      ggcggGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATtctc                                   <  2:797131/93‑1 (MQ=255)

                                                       gcggGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCg                                  <  1:748875/93‑1 (MQ=255)

                                                       gcggGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCg                                  <  2:610827/93‑1 (MQ=255)

                                                         gggCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCgga                                >  1:835354/1‑92 (MQ=255)

                                                          ggCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCa                               <  2:3997351/93‑1 (MQ=255)

                                                            ccAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCaag                             >  1:1949236/1‑93 (MQ=255)

                                                            ccAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCaag                             >  1:3611404/1‑93 (MQ=255)

                                                             cAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCaaga                            >  2:3898135/1‑93 (MQ=255)

                                                             cAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCaaga                            <  1:1456002/93‑1 (MQ=255)

                                                             cAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCAGCaaga                            >  2:2100434/1‑93 (MQ=255)

                                                              aGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCaagaa                           <  1:454094/93‑1 (MQ=255)

                                                              aGGAGCTGCTTATAAATACGGGCTGGGACGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCaagaa                           <  2:2715703/93‑1 (MQ=255)

                                                               ggAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCaagaag                          <  1:3589551/93‑1 (MQ=255)

                                                               ggAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCCGGCCTTCACTCGGTATTGTCTGAaagaag                          >  1:1377477/1‑93 (MQ=255)

                                                                gAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGt                         <  1:337628/93‑1 (MQ=255)

                                                                 aGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGtt                        <  1:1259580/93‑1 (MQ=255)

                                                                       ttATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGAt                  >  1:1785996/1‑93 (MQ=255)

                                                                       ttATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGAt                  <  1:1469834/93‑1 (MQ=255)

                                                                       ttATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTATGCGAt                  >  1:4134623/1‑93 (MQ=255)

                                                                         ataAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTc                <  2:4472575/93‑1 (MQ=255)

                                                                         ataAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTc                >  2:692592/1‑93 (MQ=255)

                                                                         ataAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTc                <  1:3220585/93‑1 (MQ=255)

                                                                         ataAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTc                <  1:585271/93‑1 (MQ=255)

                                                                          taAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCt               >  2:3868640/1‑93 (MQ=255)

                                                                          taAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCt               <  1:711335/93‑1 (MQ=255)

                                                                           aaaTACGGGCTGGGCGGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCtt              <  1:3022299/93‑1 (MQ=255)

                                                                           aaaTACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCtt              <  2:316357/93‑1 (MQ=255)

                                                                             aTACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTgc            >  1:2727460/1‑93 (MQ=255)

                                                                             aTACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTgc            <  2:52436/93‑1 (MQ=255)

                                                                             aTACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTgc            >  2:1465034/1‑93 (MQ=255)

                                                                              tACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTgcg           >  1:2692935/1‑93 (MQ=255)

                                                                              tACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTgcg           >  1:3691136/1‑93 (MQ=255)

                                                                               aCGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTgcgc          >  2:2249967/1‑93 (MQ=255)

                                                                                   gctgggccggGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGcgccg      >  1:1010414/1‑93 (MQ=255)

                                                                                    ctgggccgggTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGcgccgc     <  2:3596708/93‑1 (MQ=255)

                                                                                    ctgggccgggTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGcgccgc     <  2:1267373/93‑1 (MQ=255)

                                                                                       ggccgggTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTAc  <  1:2235657/93‑1 (MQ=255)

ATTGTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTAC  >  NC_000913/3405379‑3405558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GGTATTGTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATG  >  NC_000913/3405376‑3405562

GGTATTGTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCC                                                                                               <  SRR4302535.2003009/94‑1 (MQ=60)

GGTATTGTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACCGGCTGGGCC                                                                                               >  SRR4302535.1342444/1‑94 (MQ=60)

 GTATTGTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCG                                                                                              >  SRR4302535.1279914/1‑94 (MQ=60)

  TATTGTGCTGGCGATGACGCTCACCGCGACCTATAACAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGG                                                                                             >  SRR4302535.605202/1‑94 (MQ=60)

    TTGTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGT                                                                                           <  SRR4302535.3985103/94‑1 (MQ=60)

      GTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGGGCTGCGTATAAAAACGGGCTGGGCCGGGAAT                                                                                         >  SRR4302535.3952821/1‑94 (MQ=60)

       TGCTGGCGATGACGCTAACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATT                                                                                        <  SRR4302535.4456954/94‑1 (MQ=60)

       TGCTCGCGATGACGATCACCGCGACCTATATCAGTGCCAGTTCGTTTACCGGCGGCCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATT                                                                                        <  SRR4302535.2664652/94‑1 (MQ=60)

        GCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTG                                                                                       <  SRR4302535.4642183/94‑1 (MQ=60)

          TGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCT                                                                                     >  SRR4302535.1981773/1‑94 (MQ=60)

          TGGCGATCACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCT                                                                                     <  SRR4302535.3313218/94‑1 (MQ=60)

             CGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGGTTATAAATACGGGCTGGGCCGGGTGTTGCTtgg                                                                                  >  SRR4302535.2094068/1‑91 (MQ=60)

             CGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATTAATAAAGGCTGGGCCGGGGATTGCTGGG                                                                                  >  SRR4302535.1291874/1‑94 (MQ=60)

                  ACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTTTTAATACGCGCTGGGCCGGGTATTGCTGGCGATGT                                                                             >  SRR4302535.732462/1‑94 (MQ=60)

                      TCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTCCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCA                                                                         <  SRR4302535.2529967/94‑1 (MQ=60)

                       CACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAG                                                                        <  SRR4302535.1691445/94‑1 (MQ=60)

                       CACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAG                                                                        <  SRR4302535.1148392/94‑1 (MQ=60)

                       CACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTCAG                                                                        >  SRR4302535.3028924/1‑94 (MQ=60)

                        ACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGATGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGC                                                                       <  SRR4302535.474704/94‑1 (MQ=60)

                         CCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCT                                                                      <  SRR4302535.700310/94‑1 (MQ=60)

                            CGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGGGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGGGATGGTTCAGCTTCC                                                                   >  SRR4302535.334183/1‑94 (MQ=60)

                            CGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTTTAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTCAGCTTCC                                                                   >  SRR4302535.651016/1‑94 (MQ=60)

                             GACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCT                                                                  >  SRR4302535.2520106/1‑94 (MQ=60)

                             GACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCT                                                                  <  SRR4302535.2671642/94‑1 (MQ=60)

                             GACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCT                                                                  <  SRR4302535.3057891/94‑1 (MQ=60)

                             CACCTATATCAGTGCCAGTTCGTTTAGCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCT                                                                  <  SRR4302535.3124143/94‑1 (MQ=60)

                              ACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTG                                                                 <  SRR4302535.4282774/94‑1 (MQ=60)

                                CTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCA                                                               <  SRR4302535.1551685/94‑1 (MQ=60)

                                 TATATCAGTGCTAGTTCGTTTATCGGCGGGCCAGGATCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAG                                                              <  SRR4302535.299606/94‑1 (MQ=60)

                                  ATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTCAGCTTCCTGCAGT                                                             >  SRR4302535.3751864/1‑94 (MQ=60)

                                   TATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGGGCTGCTTATAAATACGGGCTGGGCCGGGGGTTGCTGGCGATGATTCTGCTTCCTGCAGTC                                                            >  SRR4302535.3511056/1‑94 (MQ=60)

                                   TATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTTAGCTTCCTTCAGTC                                                            >  SRR4302535.1618536/1‑94 (MQ=60)

                                    ATCAGTGCCAGTTCGTTTATCTGCGGGCCAGGGGCCGCTTATAAATACGGGGTGGGCCGGGGATTTCTGGCGACGATTCAcgtttcaccagtct                                                           >  SRR4302535.768162/1‑80 (MQ=39)

                                    ATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCT                                                           <  SRR4302535.2931831/94‑1 (MQ=60)

                                    ATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCT                                                           <  SRR4302535.493613/94‑1 (MQ=60)

                                    ATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCT                                                           <  SRR4302535.221856/94‑1 (MQ=60)

                                     TCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCCGGCATTGGTGGCCATGAGTCAGCTTCCTGCAGCCTG                                                          >  SRR4302535.785819/1‑94 (MQ=60)

                                       AGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGC                                                        <  SRR4302535.1655125/94‑1 (MQ=60)

                                       AGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGC                                                        >  SRR4302535.1267692/1‑94 (MQ=60)

                                        GTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCT                                                       <  SRR4302535.1804108/94‑1 (MQ=60)

                                        GTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCT                                                       <  SRR4302535.3228325/94‑1 (MQ=60)

                                        GTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTTGCGATGATTCAGCTTCCTGCAGTCTGGCT                                                       >  SRR4302535.1904354/1‑94 (MQ=60)

                                         TGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTT                                                      <  SRR4302535.1190856/94‑1 (MQ=60)

                                          GCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGATTT                                                     <  SRR4302535.3888689/94‑1 (MQ=60)

                                           CCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTC                                                    <  SRR4302535.4002156/94‑1 (MQ=60)

                                             AGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCAC                                                  <  SRR4302535.1470403/94‑1 (MQ=60)

                                             AGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCAC                                                  >  SRR4302535.3157083/1‑94 (MQ=60)

                                              GTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACT                                                 <  SRR4302535.1499937/94‑1 (MQ=60)

                                               TTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTC                                                >  SRR4302535.2099460/1‑94 (MQ=60)

                                               TTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTC                                                >  SRR4302535.298167/1‑94 (MQ=60)

                                                 CGTTTATCGGCGGGCCCGGGGCCGCTTATAAATACCGGCTCGGCCGGGGATTGCTGGCGATGATTCAGCCTCCTGCAGTCTGGGTTTCTATCGG                                              >  SRR4302535.4013121/1‑94 (MQ=40)

                                                  GTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATGGTTCAGCTTCCTGCAGTCTGGCTTTTACTCGGT                                             >  SRR4302535.3254319/1‑94 (MQ=60)

                                                  GTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGT                                             >  SRR4302535.1603580/1‑94 (MQ=60)

                                                    TTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTAT                                           <  SRR4302535.1736107/94‑1 (MQ=60)

                                                    TTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGGATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTAT                                           >  SRR4302535.2657477/1‑94 (MQ=60)

                                                     TATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATT                                          <  SRR4302535.3891294/94‑1 (MQ=60)

                                                     TATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATT                                          <  SRR4302535.977019/94‑1 (MQ=60)

                                                     TATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATT                                          <  SRR4302535.232381/94‑1 (MQ=60)

                                                       TCGGCGTGCCAGGAGCTGCTTATAAATACGGGCTTGGTCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCT                                        <  SRR4302535.2752483/94‑1 (MQ=60)

                                                        CGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTC                                       <  SRR4302535.10819/94‑1 (MQ=60)

                                                        CGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTC                                       >  SRR4302535.218900/1‑94 (MQ=60)

                                                         GGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCG                                      <  SRR4302535.4593862/94‑1 (MQ=60)

                                                          GCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGG                                     <  SRR4302535.754142/94‑1 (MQ=60)

                                                           CGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGA                                    >  SRR4302535.841213/1‑94 (MQ=60)

                                                            GGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCA                                   <  SRR4302535.3692614/94‑1 (MQ=60)

                                                            GGGCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCA                                   >  SRR4302535.449154/1‑94 (MQ=60)

                                                              GCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAG                                 >  SRR4302535.3636447/1‑94 (MQ=60)

                                                              GCCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAG                                 >  SRR4302535.1963267/1‑94 (MQ=60)

                                                               CCAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGA                                >  SRR4302535.213912/1‑94 (MQ=60)

                                                                CAGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAA                               <  SRR4302535.1466424/94‑1 (MQ=60)

                                                                 AGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAG                              <  SRR4302535.2400581/94‑1 (MQ=60)

                                                                 AGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAG                              <  SRR4302535.457276/94‑1 (MQ=60)

                                                                 AGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAG                              >  SRR4302535.1904507/1‑94 (MQ=60)

                                                                 AGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAG                              >  SRR4302535.272178/1‑94 (MQ=60)

                                                                 AGGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCCGGCCTTCACTCGGTATTGTCTGAAAGAAG                              >  SRR4302535.1387306/1‑94 (MQ=60)

                                                                  GGAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGT                             <  SRR4302535.3614452/94‑1 (MQ=60)

                                                                   GAGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTT                            <  SRR4302535.339964/94‑1 (MQ=60)

                                                                    AGCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTT                           <  SRR4302535.1268595/94‑1 (MQ=60)

                                                                     GCTGCTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTG                          <  SRR4302535.2276630/94‑1 (MQ=60)

                                                                         CTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGAT                      >  SRR4302535.1798921/1‑94 (MQ=60)

                                                                         CTTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTATGCGAT                      >  SRR4302535.4162936/1‑94 (MQ=60)

                                                                          TTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGTAAGAAGTTTGCGATT                     >  SRR4302535.3635919/1‑94 (MQ=60)

                                                                          TTATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATT                     <  SRR4302535.1480362/94‑1 (MQ=60)

                                                                            ATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCT                   >  SRR4302535.1412193/1‑94 (MQ=60)

                                                                            ATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCT                   <  SRR4302535.3774114/94‑1 (MQ=60)

                                                                            ATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCT                   <  SRR4302535.589373/94‑1 (MQ=60)

                                                                            ATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCT                   >  SRR4302535.1083080/1‑94 (MQ=60)

                                                                            ATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCT                   <  SRR4302535.3242902/94‑1 (MQ=60)

                                                                            ATAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCGTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCT                   <  SRR4302535.1793981/94‑1 (MQ=60)

                                                                             TAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTT                  <  SRR4302535.716330/94‑1 (MQ=60)

                                                                             TAAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTT                  <  SRR4302535.1495493/94‑1 (MQ=60)

                                                                              AAATACGGGCTGGGCGGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTG                 <  SRR4302535.3043460/94‑1 (MQ=60)

                                                                              AAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTG                 <  SRR4302535.3719806/94‑1 (MQ=60)

                                                                              AAATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTG                 >  SRR4302535.797529/1‑94 (MQ=60)

                                                                               AATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGC                >  SRR4302535.2746937/1‑94 (MQ=60)

                                                                                ATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCG               >  SRR4302535.2712182/1‑94 (MQ=60)

                                                                                ATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCG               >  SRR4302535.3716682/1‑94 (MQ=60)

                                                                                ATACGGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCG               <  SRR4302535.412188/94‑1 (MQ=60)

                                                                                    GGGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCC           <  SRR4302535.3574993/94‑1 (MQ=60)

                                                                                     GGCTGGGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCG          >  SRR4302535.1017591/1‑94 (MQ=60)

                                                                                          GGCCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACA     <  SRR4302535.2251561/94‑1 (MQ=60)

                                                                                            CCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAAT   <  SRR4302535.804893/94‑1 (MQ=60)

                                                                                            CCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAAT   >  SRR4302535.3737380/1‑94 (MQ=60)

                                                                                            CCGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGATACAAT   >  SRR4302535.642849/1‑94 (MQ=60)

                                                                                             CGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATG  <  SRR4302535.1265275/94‑1 (MQ=60)

                                                                                             CGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATG  <  SRR4302535.3036034/94‑1 (MQ=60)

GGTATTGTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATG  >  NC_000913/3405376‑3405562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`