Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,427,261	T→C	V130A (GTC→GCC)	b3279 →	putative transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,427,261	0	T	C	99.5%	600.6 / NA	190	V130A (GTC→GCC)	b3279	putative transferase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (92/96); minor base G (1/0); total (93/97)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 201 total aligned reads displayed.

TTGGCAATCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGTCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAGGCTG  >  NC_000913/3427171‑3427350

 tGGCAATCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGcccc                                                                                        >  1:3902896/1‑93 (MQ=255)

  ggCAATCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCca                                                                                       >  2:2734989/1‑93 (MQ=255)

   gCAATCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCcac                                                                                      <  1:353849/93‑1 (MQ=255)

     aaTCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACaa                                                                                    >  1:721297/1‑93 (MQ=255)

      aTCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACaaa                                                                                   >  2:1002687/1‑93 (MQ=255)

       tCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGTGTAGTCTGGCCCCACaaaa                                                                                  >  1:1657754/1‑93 (MQ=255)

       tCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACaaaa                                                                                  <  1:1728822/93‑1 (MQ=255)

        cGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAAt                                                                                 <  2:3324450/93‑1 (MQ=255)

        cGAGTTGTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAAt                                                                                 <  1:1022608/93‑1 (MQ=255)

          aGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATaa                                                                               <  1:3679126/93‑1 (MQ=255)

           gTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATaaa                                                                              <  2:2629556/93‑1 (MQ=255)

           gTTGTGGTGGGGATGTGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGACCCACAAAATaaa                                                                              <  2:3549466/93‑1 (MQ=255)

            ttttGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAAc                                                                             >  1:1520463/1‑93 (MQ=255)

            ttttGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAAc                                                                             >  2:2280332/1‑93 (MQ=255)

             tttGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACg                                                                            <  1:4103176/93‑1 (MQ=255)

              ttggttggGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACgg                                                                           >  2:4483977/1‑93 (MQ=255)

                ggttggGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCt                                                                         <  2:315032/93‑1 (MQ=255)

                   tggGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGga                                                                      <  2:1887375/93‑1 (MQ=255)

                   tggGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGga                                                                      <  1:2826981/93‑1 (MQ=255)

                    gggATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGgag                                                                     >  2:2804636/1‑93 (MQ=255)

                       aTGGTCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGc                                                                  <  2:3094192/93‑1 (MQ=255)

                       aTGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGc                                                                  <  2:2080936/93‑1 (MQ=255)

                        tGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCg                                                                 <  2:3183246/93‑1 (MQ=255)

                        tGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCg                                                                 >  1:3422230/1‑93 (MQ=255)

                          ggCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTTGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGa                                                               <  2:3819506/93‑1 (MQ=255)

                          ggCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGa                                                               <  1:3879779/93‑1 (MQ=255)

                           gCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGat                                                              <  1:188316/93‑1 (MQ=255)

                            cTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGata                                                             <  2:3280908/93‑1 (MQ=255)

                             tCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGatat                                                            >  2:798982/1‑93 (MQ=255)

                              cAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATc                                                           <  1:2902430/93‑1 (MQ=255)

                              cAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATc                                                           <  2:632966/93‑1 (MQ=255)

                               aaTTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCt                                                          >  1:4122917/1‑93 (MQ=255)

                                aTTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTg                                                         >  1:705518/1‑93 (MQ=255)

                                aTTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTg                                                         <  1:4572380/93‑1 (MQ=255)

                                aTTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTg                                                         <  1:2256015/93‑1 (MQ=255)

                                aTTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTg                                                         >  2:4122447/1‑93 (MQ=255)

                                aTTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTg                                                         <  1:1286554/93‑1 (MQ=255)

                                 ttttACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGt                                                        >  1:592825/1‑93 (MQ=255)

                                 ttttACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGt                                                        >  1:4521651/1‑93 (MQ=255)

                                     aCTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTAtct                                                    >  1:1197645/1‑93 (MQ=255)

                                       ttGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCg                                                  >  1:3270552/1‑93 (MQ=255)

                                         gATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGt                                                <  1:4006732/93‑1 (MQ=255)

                                          aTGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTa                                               <  1:3609143/93‑1 (MQ=255)

                                           tGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAg                                              >  2:1768813/1‑93 (MQ=255)

                                              cgcAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGccc                                           <  2:4187024/93‑1 (MQ=255)

                                              cgcAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGccc                                           <  1:1125475/93‑1 (MQ=255)

                                               gcAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGcccc                                          >  2:3088279/1‑93 (MQ=255)

                                                cAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGCGAGCGGATATCTGTATCTCGGTAGCCCCg                                         >  2:1470444/1‑93 (MQ=255)

                                                cAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCg                                         >  1:1318323/1‑93 (MQ=255)

                                                cAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCg                                         <  2:3903127/93‑1 (MQ=255)

                                                 aaTAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGt                                        <  2:293290/93‑1 (MQ=255)

                                                 aaTAGTAGAAGATGATGTGATGATTGGCGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGt                                        >  2:2142316/1‑93 (MQ=255)

                                                  aTAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTc                                       >  1:1074108/1‑93 (MQ=255)

                                                   tagtagAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCa                                      <  2:1747663/93‑1 (MQ=255)

                                                   tagtagAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCa                                      >  2:1602388/1‑93 (MQ=255)

                                                   tagtagAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCa                                      >  2:1259808/1‑93 (MQ=255)

                                                    agtagAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCaa                                     >  1:1960099/1‑93 (MQ=255)

                                                     gtagAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCaaa                                    >  2:4652577/1‑93 (MQ=255)

                                                      tagAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAAc                                   <  1:530311/93‑1 (MQ=255)

                                                        gaagaTGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAg                                 >  2:1379554/1‑93 (MQ=255)

                                                        gaagaTGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAg                                 >  1:268232/1‑93 (MQ=255)

                                                        gaagaTGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAg                                 >  2:1649147/1‑93 (MQ=255)

                                                        gaagaTGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAg                                 >  1:3656333/1‑93 (MQ=255)

                                                          agaTGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGAt                               >  2:681931/1‑93 (MQ=255)

                                                          agaTGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGAt                               <  1:3023222/93‑1 (MQ=255)

                                                           gatgatGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATc                              <  2:924331/93‑1 (MQ=255)

                                                            atgatgTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATcc                             >  1:3734146/1‑93 (MQ=255)

                                                             tgatgtgatgATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCg                            >  1:1573992/1‑93 (MQ=255)

                                                              gatgtgatgATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGc                           >  1:3224258/1‑93 (MQ=255)

                                                               atgtgatgATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTCTCTCGGTAGCCCCGTCAAACAGATCCGcc                          >  1:1399005/1‑93 (MQ=255)

                                                               atgtgatgATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGcc                          <  2:1087688/93‑1 (MQ=255)

                                                               atgtgatgATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGcc                          >  1:4654180/1‑93 (MQ=255)

                                                               atgtgatgATTGGTGCGGGTAGTCAGGCCCCACAAAATAAACGTCTGGAGAGCGGATATCTTTATCTCGGTAGCCCCGTCAAACAGGTCCGcc                          <  2:538652/93‑1 (MQ=255)

                                                                   gatgatTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGtt                      >  1:3277116/1‑93 (MQ=255)

                                                                       atTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGt                  >  1:432633/1‑93 (MQ=255)

                                                                       atTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGt                  <  1:3314207/93‑1 (MQ=255)

                                                                        tgggTGCGGTTAGTCGGCCCCCAAAAAATAACCGGCGGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGtg                 <  2:3737179/91‑1 (MQ=255)

                                                                         tGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGtgt                >  1:2332364/1‑92 (MQ=255)

                                                                         tGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGtga                <  1:755929/93‑1 (MQ=255)

                                                                          tgTGCGGATATTCTGCCCCCAAAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGtgat               <  2:2480310/92‑1 (MQ=255)

                                                                          ggTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGtgat               <  1:3243968/93‑1 (MQ=255)

                                                                           gTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGtgatg              <  1:4136809/93‑1 (MQ=255)

                                                                           gTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGtgatg              <  1:291692/93‑1 (MQ=255)

                                                                             gCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGaa            <  1:4460350/93‑1 (MQ=255)

                                                                             gCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGaa            <  2:2648795/93‑1 (MQ=255)

                                                                              cGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAag           >  1:1343875/1‑93 (MQ=255)

                                                                               gggTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAaga          >  1:1787521/1‑93 (MQ=255)

                                                                               gggTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAaga          >  2:2209806/1‑93 (MQ=255)

                                                                               gggTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAaga          <  2:186080/93‑1 (MQ=255)

                                                                                ggTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAagag         <  1:647765/93‑1 (MQ=255)

                                                                                ggTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAagag         <  1:2576068/93‑1 (MQ=255)

                                                                                ggTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAagag         >  1:355791/1‑93 (MQ=255)

                                                                                 gTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAgaga        <  2:2364432/93‑1 (MQ=255)

                                                                                 gTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAgaga        <  1:1461416/93‑1 (MQ=255)

                                                                                  tAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGaa       <  1:3393970/93‑1 (MQ=255)

                                                                                  tAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGaa       <  1:1908402/93‑1 (MQ=255)

                                                                                  tAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGaa       >  2:4550735/1‑93 (MQ=255)

                                                                                    gTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAgg     >  2:1048746/1‑93 (MQ=255)

                                                                                      cTGGCCCCACAAAATAACCGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGACAAGGCt   <  2:2018030/93‑1 (MQ=255)

                                                                                       tGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAGGCTg  <  1:1144549/93‑1 (MQ=255)

TTGGCAATCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGTCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAGGCTG  >  NC_000913/3427171‑3427350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCATTGGCAATCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGTCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAGGCTGGGTT  >  NC_000913/3427168‑3427354

CCATTGGCAATCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGC                                                                                               >  SRR4302535.1562726/1‑94 (MQ=60)

 CATTGGCAATCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCC                                                                                              >  SRR4302535.2244342/1‑94 (MQ=60)

   TTGGCAATCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCC                                                                                            >  SRR4302535.3929633/1‑94 (MQ=60)

      GCAATCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACA                                                                                         <  SRR4302535.356293/94‑1 (MQ=60)

      GCAATCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACA                                                                                         >  SRR4302535.1349095/1‑94 (MQ=60)

       CAATCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAA                                                                                        >  SRR4302535.726361/1‑94 (MQ=60)

        AATCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAA                                                                                       >  SRR4302535.3401435/1‑94 (MQ=60)

        AATCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAA                                                                                       >  SRR4302535.3611303/1‑94 (MQ=60)

         ATCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGTGTAGTCTGGCCCCACAAAA                                                                                      >  SRR4302535.1669742/1‑94 (MQ=60)

         ATCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAA                                                                                      >  SRR4302535.4139674/1‑94 (MQ=60)

          TCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAAT                                                                                     <  SRR4302535.2517542/94‑1 (MQ=60)

          TCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAAT                                                                                     <  SRR4302535.163015/94‑1 (MQ=60)

          TCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAAT                                                                                     <  SRR4302535.1741315/94‑1 (MQ=60)

           CGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATA                                                                                    <  SRR4302535.2702813/94‑1 (MQ=60)

           CGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATA                                                                                    <  SRR4302535.2890709/94‑1 (MQ=60)

           CGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATA                                                                                    <  SRR4302535.400177/94‑1 (MQ=60)

           CGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATA                                                                                    <  SRR4302535.3246939/94‑1 (MQ=60)

           CGAGTTGTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATA                                                                                    <  SRR4302535.1029872/94‑1 (MQ=60)

             AGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAA                                                                                  <  SRR4302535.3704591/94‑1 (MQ=60)

              GTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAAC                                                                                 <  SRR4302535.3425310/94‑1 (MQ=60)

              GTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAAC                                                                                 >  SRR4302535.1531416/1‑94 (MQ=60)

               TTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACG                                                                                <  SRR4302535.3270007/94‑1 (MQ=60)

               TTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACG                                                                                <  SRR4302535.694567/94‑1 (MQ=60)

                TTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGG                                                                               <  SRR4302535.4131268/94‑1 (MQ=60)

                TTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGG                                                                               <  SRR4302535.2299789/94‑1 (MQ=60)

                  GGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCT                                                                             <  SRR4302535.1741129/94‑1 (MQ=60)

                    GTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGG                                                                           <  SRR4302535.1380887/94‑1 (MQ=60)

                    GTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGG                                                                           <  SRR4302535.1087941/94‑1 (MQ=60)

                      TGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAG                                                                         <  SRR4302535.2847044/94‑1 (MQ=60)

                        GGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAG                                                                       >  SRR4302535.3807874/1‑94 (MQ=60)

                         GATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGC                                                                      <  SRR4302535.1446741/94‑1 (MQ=60)

                          gttgggttAATTTTACTTTATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCG                                                                     <  SRR4302535.1311815/86‑1 (MQ=60)

                          ATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCG                                                                     >  SRR4302535.3446034/1‑94 (MQ=60)

                           TGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGG                                                                    >  SRR4302535.1909845/1‑94 (MQ=60)

                             GGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGAT                                                                  <  SRR4302535.3906410/94‑1 (MQ=60)

                              GCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATA                                                                 <  SRR4302535.189619/94‑1 (MQ=60)

                               CTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATAT                                                                >  SRR4302535.368410/1‑94 (MQ=60)

                                 CAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCT                                                              >  SRR4302535.4151129/1‑94 (MQ=60)

                                 CAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCT                                                              <  SRR4302535.2922947/94‑1 (MQ=60)

                                 CAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCT                                                              <  SRR4302535.3131773/94‑1 (MQ=60)

                                  AATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTG                                                             >  SRR4302535.710473/1‑94 (MQ=60)

                                   ATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGT                                                            <  SRR4302535.1295721/94‑1 (MQ=60)

                                   ATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGT                                                            >  SRR4302535.1309964/1‑94 (MQ=60)

                                   ATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGT                                                            >  SRR4302535.750200/1‑94 (MQ=60)

                                   ATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGT                                                            <  SRR4302535.2272078/94‑1 (MQ=60)

                                   ATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGT                                                            <  SRR4302535.4603551/94‑1 (MQ=60)

                                   ATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGT                                                            >  SRR4302535.596979/1‑94 (MQ=60)

                                   ATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGT                                                            <  SRR4302535.3574203/94‑1 (MQ=60)

                                   ATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGT                                                            >  SRR4302535.4552636/1‑94 (MQ=60)

                                   ATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGT                                                            >  SRR4302535.4552637/1‑94 (MQ=60)

                                    TTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTA                                                           <  SRR4302535.2924237/94‑1 (MQ=60)

                                       TACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCT                                                        >  SRR4302535.1206221/1‑94 (MQ=60)

                                        TCTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTC                                                       <  SRR4302535.1933114/94‑1 (MQ=60)

                                        ACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGACCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTC                                                       >  SRR4302535.1137885/1‑94 (MQ=60)

                                         CTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCG                                                      >  SRR4302535.3293270/1‑94 (MQ=60)

                                          TTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGG                                                     <  SRR4302535.3671134/94‑1 (MQ=60)

                                           TGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAACCGGCTGGAGAGCGGATATCTTTATCTCGGA                                                    >  SRR4302535.1283572/1‑94 (MQ=60)

                                            GATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTA                                                   <  SRR4302535.4034146/94‑1 (MQ=60)

                                             ATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAG                                                  <  SRR4302535.3634176/94‑1 (MQ=60)

                                                GCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCC                                               <  SRR4302535.4391182/94‑1 (MQ=60)

                                                 CGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCC                                              <  SRR4302535.1133510/94‑1 (MQ=60)

                                                  GCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCG                                             >  SRR4302535.1327743/1‑94 (MQ=60)

                                                   CAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGT                                            >  SRR4302535.4702611/1‑94 (MQ=60)

                                                    AATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTC                                           <  SRR4302535.2062688/94‑1 (MQ=60)

                                                    AATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTC                                           >  SRR4302535.1081745/1‑94 (MQ=60)

                                                     ATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCA                                          >  SRR4302535.152957/1‑94 (MQ=60)

                                                      TAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAA                                         >  SRR4302535.1974237/1‑94 (MQ=60)

                                                       AGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAA                                        >  SRR4302535.4578203/1‑94 (MQ=60)

                                                       AGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAA                                        >  SRR4302535.1060137/1‑94 (MQ=60)

                                                         TAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACA                                      >  SRR4302535.775729/1‑94 (MQ=60)

                                                         TAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACA                                      <  SRR4302535.534020/94‑1 (MQ=60)

                                                         TAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACA                                      <  SRR4302535.1161733/94‑1 (MQ=60)

                                                          AGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAG                                     <  SRR4302535.1573869/94‑1 (MQ=60)

                                                          AGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAG                                     >  SRR4302535.270094/1‑94 (MQ=60)

                                                          AGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAG                                     >  SRR4302535.3681642/1‑94 (MQ=60)

                                                             AGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATC                                  <  SRR4302535.3044389/94‑1 (MQ=60)

                                                              GATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCC                                 >  SRR4302535.3717746/1‑94 (MQ=60)

                                                              GATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCC                                 >  SRR4302535.3759992/1‑94 (MQ=60)

                                                              GATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCC                                 >  SRR4302535.644194/1‑94 (MQ=60)

                                                               ATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAGCGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCG                                >  SRR4302535.3315479/1‑94 (MQ=60)

                                                               ATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCG                                >  SRR4302535.715502/1‑94 (MQ=60)

                                                               ATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCG                                >  SRR4302535.1585355/1‑94 (MQ=60)

                                                                TGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGC                               >  SRR4302535.3246610/1‑94 (MQ=60)

                                                                TGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGC                               >  SRR4302535.4659319/1‑94 (MQ=60)

                                                                 GATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTCTCTCGGTAGCCCCGTCAAACAGATCCGCC                              >  SRR4302535.1408992/1‑94 (MQ=60)

                                                                 GATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCC                              >  SRR4302535.4685687/1‑94 (MQ=60)

                                                                  ATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCACGGTAGCCCCGTCAAACAGATCCGCCC                             >  SRR4302535.2610840/1‑94 (MQ=60)

                                                                   TGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCG                            >  SRR4302535.3295112/1‑94 (MQ=60)

                                                                     TGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTT                          >  SRR4302535.3299881/1‑94 (MQ=60)

                                                                       ATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAA                        >  SRR4302535.2953685/1‑94 (MQ=60)

                                                                       ATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAA                        >  SRR4302535.404381/1‑94 (MQ=60)

                                                                         GATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGT                      >  SRR4302535.435646/1‑94 (MQ=60)

                                                                          ATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTG                     <  SRR4302535.3337265/94‑1 (MQ=60)

                                                                           TTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGT                    >  SRR4302535.2349049/1‑94 (MQ=60)

                                                                            TGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGAT                   <  SRR4302535.761252/94‑1 (MQ=60)

                                                                            TGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGAT                   <  SRR4302535.1742632/94‑1 (MQ=60)

                                                                             GGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATG                  <  SRR4302535.3266502/94‑1 (MQ=60)

                                                                              GTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGA                 <  SRR4302535.293733/94‑1 (MQ=60)

                                                                              GTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGA                 <  SRR4302535.4165136/94‑1 (MQ=60)

                                                                                GCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAG               >  SRR4302535.1353469/1‑94 (MQ=60)

                                                                                GCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAG               <  SRR4302535.4490942/94‑1 (MQ=60)

                                                                                GCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAG               <  SRR4302535.3910244/94‑1 (MQ=60)

                                                                                 CGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGA              >  SRR4302535.1800449/1‑94 (MQ=60)

                                                                                 CGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGAGGATATCTGTATCTagaccacgcagatcgcaagagccgttcagcaggaaatccgag              >  SRR4302535.286511/1‑52 (MQ=60)

                                                                                  GGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAG             >  SRR4302535.358244/1‑94 (MQ=60)

                                                                                   GGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGA            <  SRR4302535.652280/94‑1 (MQ=60)

                                                                                   GGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGA            <  SRR4302535.2594582/94‑1 (MQ=60)

                                                                                    GTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAA           <  SRR4302535.1471886/94‑1 (MQ=60)

                                                                                     TAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAG          <  SRR4302535.1922160/94‑1 (MQ=60)

                                                                                     TAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAG          <  SRR4302535.3417584/94‑1 (MQ=60)

                                                                                     TAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAG          <  SRR4302535.1891803/94‑1 (MQ=60)

                                                                                      AGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAGG         <  SRR4302535.4156102/94‑1 (MQ=60)

                                                                                        TCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAGGCT       <  SRR4302535.4261464/94‑1 (MQ=60)

                                                                                         CTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAGGCTG      >  SRR4302535.552525/1‑94 (MQ=60)

                                                                                          TGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAGGCTGG     <  SRR4302535.3523654/94‑1 (MQ=60)

                                                                                          TGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAGGCTGG     <  SRR4302535.1152709/94‑1 (MQ=60)

                                                                                            GCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAGGCTGGGT   >  SRR4302535.1074554/1‑94 (MQ=60)

                                                                                             CCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAGGCTGGGTT  >  SRR4302535.4111439/1‑94 (MQ=60)

                                                                                             CCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAGGCTGGGTT  >  SRR4302535.2295902/1‑94 (MQ=60)

CCATTGGCAATCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGTCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAGGCTGGGTT  >  NC_000913/3427168‑3427354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`