Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,430,453	+C	coding (745/747 nt)	smf ←	putative Rossmann‑fold nucleotide‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,430,453	1	.	C	98.9%	564.9 / ‑1.6	184	coding (745/747 nt)	smf	putative Rossmann‑fold nucleotide‑binding protein
Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (102/80); total (103/81)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 206 total aligned reads displayed.

GAATTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑G‑AAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTT  >  NC_000913/3430361‑3430539

gAATTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑g‑g                                                                                         >  2:570119/1‑93 (MQ=255)

 aaTTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGCCCTGGCAA‑g‑gc                                                                                        >  2:114572/1‑92 (MQ=255)

 aaTTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑g‑gc                                                                                        >  1:1609555/1‑92 (MQ=255)

  aTTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑g‑gca                                                                                       >  2:4107274/1‑91 (MQ=255)

  aTTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑g‑gca                                                                                       >  1:1628322/1‑91 (MQ=255)

    ttcttcCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑G‑caaa                                                                                    >  1:3155953/1‑93 (MQ=255)

    ttcttcCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑G‑caaa                                                                                    <  2:375000/93‑1 (MQ=255)

     tcttcCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑G‑caaaa                                                                                   >  1:2520525/1‑93 (MQ=255)

      cttcCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑G‑caaaaa                                                                                  <  1:522745/93‑2 (MQ=255)

       ttcCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑G‑caaaaac                                                                                 >  2:4368348/1‑91 (MQ=255)

       ttcCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑G‑caaaaac                                                                                 >  2:4104070/1‑91 (MQ=255)

         ccGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTTGACCTGGCAA‑G‑GCAAAAACtc                                                                                >  2:3443561/1‑92 (MQ=255)

          cGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTtc                                                                               <  1:747489/93‑1 (MQ=255)

          cGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTtc                                                                               <  1:548948/93‑1 (MQ=255)

           ggTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTtct                                                                              <  2:2844731/93‑1 (MQ=255)

           ggTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTtct                                                                              <  1:358962/93‑1 (MQ=255)

            gTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTtctc                                                                             >  1:192148/1‑93 (MQ=255)

            gTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTtctc                                                                             >  1:9176/1‑93 (MQ=255)

            gTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTtctc                                                                             <  2:4589646/93‑1 (MQ=255)

            gTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTtctc                                                                             <  2:4649395/93‑1 (MQ=255)

            gTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTtctc                                                                             >  1:339295/1‑93 (MQ=255)

            gTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTtctc                                                                             <  1:1010487/93‑1 (MQ=255)

             ttCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCg                                                                            >  1:1419918/1‑93 (MQ=255)

               ccGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGcc                                                                          >  2:2818396/1‑93 (MQ=255)

                  tCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCt                                                                       >  1:104755/1‑93 (MQ=255)

                    acaAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGc                                                                     <  2:1497745/93‑1 (MQ=255)

                    acaAGAATCGCACCTTGTTTTATTAACCAGTGAGGACCTTCGCTTCCCGGATTCCCTCTTGGCCCTGGCAA‑G‑GCCAAAACTTCTCCCCGCTGc                                                                     >  1:2760762/1‑93 (MQ=255)

                      aaGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTc                                                                   >  2:68883/1‑93 (MQ=255)

                       aGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCa                                                                  >  2:1935754/1‑93 (MQ=255)

                        gAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCaa                                                                 >  1:1798030/1‑93 (MQ=255)

                         aaTCGCACCTTGTTTTATTAACCAGTGAGGACCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAg                                                                >  2:2423838/1‑93 (MQ=255)

                          aTCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAgc                                                               <  1:4469994/93‑1 (MQ=255)

                          aTCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAgc                                                               >  2:1469624/1‑93 (MQ=255)

                           tCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGG‑G‑AAACACTTCTCGCCCCTGCTCAAgcg                                                              >  2:1193354/1‑93 (MQ=255)

                           tCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAgcg                                                              <  1:1720412/93‑1 (MQ=255)

                             gCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGca                                                            <  1:2020107/93‑1 (MQ=255)

                             gCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGca                                                            <  1:1474214/93‑1 (MQ=255)

                               aCCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGcaca                                                          >  1:2555954/1‑93 (MQ=255)

                               aCCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGcaca                                                          >  2:1300754/1‑93 (MQ=255)

                               aCCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGcaca                                                          >  2:1952962/1‑93 (MQ=255)

                                ccTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACaa                                                         >  1:2473704/1‑93 (MQ=255)

                                ccTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACaa                                                         >  2:276149/1‑93 (MQ=255)

                                ccTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACaa                                                         >  2:2450173/1‑93 (MQ=255)

                                      tttATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCt                                                   <  2:3121378/93‑1 (MQ=255)

                                       ttattaACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTg                                                  >  1:4258448/1‑93 (MQ=255)

                                       ttattaACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTg                                                  >  1:3282614/1‑93 (MQ=255)

                                        tattaACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTTCTGt                                                 <  2:168816/93‑1 (MQ=255)

                                         attaACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTc                                                <  2:423004/93‑1 (MQ=255)

                                          ttaACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCa                                               >  2:1996067/1‑93 (MQ=255)

                                           taACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCAc                                              >  2:3601441/1‑93 (MQ=255)

                                             aCCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTTCTGTCACCa                                            <  1:2730195/93‑1 (MQ=255)

                                              ccAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAg                                           <  1:3429814/93‑1 (MQ=255)

                                               cAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGc                                          >  1:2133742/1‑93 (MQ=255)

                                                aGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCg                                         >  1:857999/1‑93 (MQ=255)

                                                 gTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGa                                        <  2:2250773/93‑1 (MQ=255)

                                                     ggCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCa                                    >  2:1606457/1‑93 (MQ=255)

                                                     ggCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCa                                    <  2:204624/93‑1 (MQ=255)

                                                      gCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCAc                                   <  1:3524056/93‑1 (MQ=255)

                                                       cccTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACt                                  >  1:287920/1‑93 (MQ=255)

                                                       cccTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACt                                  >  1:1672543/1‑93 (MQ=255)

                                                         cTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑GTC‑AAAAACTCCTCGCCCCTGCTCAAGCGCACACCGTGCTGTCACCAGCGAACCACTAc                                <  2:2740785/93‑1 (MQ=255)

                                                         cTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTAc                                >  2:1749927/1‑93 (MQ=255)

                                                         cTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTAc                                >  2:1776143/1‑93 (MQ=255)

                                                         cTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTAc                                >  1:1324945/1‑93 (MQ=255)

                                                         cTTCGCTTCCCGGATTCCCTATGGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTAc                                <  2:2503713/93‑1 (MQ=255)

                                                          ttCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTAcc                               >  2:2184296/1‑92 (MQ=255)

                                                          ttCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACg                               >  1:664209/1‑93 (MQ=255)

                                                          ttCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACg                               >  2:720475/1‑93 (MQ=255)

                                                           tCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGc                              >  2:3473221/1‑93 (MQ=255)

                                                           tCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGc                              >  1:3937099/1‑93 (MQ=255)

                                                              cTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTTCTGTCACCAGCGAACCACTACGCaaa                           <  1:3491633/93‑1 (MQ=255)

                                                              cTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCaaa                           <  2:1099234/93‑1 (MQ=255)

                                                              cTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCaaa                           <  1:3715445/93‑1 (MQ=255)

                                                              cTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCaaa                           <  1:2406688/93‑1 (MQ=255)

                                                                tCCCGGGTTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGc                         >  2:52821/1‑93 (MQ=255)

                                                                tCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGc                         <  1:2657799/93‑1 (MQ=255)

                                                                tCCCGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGc                         <  2:1877325/93‑1 (MQ=255)

                                                                  ccGGATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCg                       >  2:3653226/1‑93 (MQ=255)

                                                                    ggATTCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCt                     <  1:2681127/93‑1 (MQ=255)

                                                                       ttCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTcc                  >  1:310596/1‑93 (MQ=255)

                                                                        tCCCTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTAGGCAAAGCCGCTTccc                 >  1:4145336/1‑92 (MQ=255)

                                                                         cccTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTccac                >  2:1430871/1‑93 (MQ=255)

                                                                          ccTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTccacc               <  1:1260432/93‑1 (MQ=255)

                                                                           cTATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTccacca              >  2:1569065/1‑93 (MQ=255)

                                                                            tATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTccaccac             <  2:1333371/93‑1 (MQ=255)

                                                                            tATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTccaccac             >  1:3748867/1‑93 (MQ=255)

                                                                            tATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTccaccac             >  2:263318/1‑93 (MQ=255)

                                                                            tATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTccaccac             >  1:504607/1‑93 (MQ=255)

                                                                            tATTGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTccaccac             <  1:1467289/93‑1 (MQ=255)

                                                                               tGGACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGCACCACTACGCAAAGCCGCTTCCACCACCAg          >  2:2989838/1‑93 (MQ=255)

                                                                                 gACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTTCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTa        <  1:1201171/93‑1 (MQ=255)

                                                                                 gACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTa        <  2:1492499/93‑1 (MQ=255)

                                                                                 gACCTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTa        <  2:3696761/93‑1 (MQ=255)

                                                                                   ccTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTaca      <  1:4027333/93‑1 (MQ=255)

                                                                                    cTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTacac     >  1:598273/1‑93 (MQ=255)

                                                                                    cTGGCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTacac     >  1:644955/1‑93 (MQ=255)

                                                                                      ggcaa‑g‑gcaaAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCt   >  1:70217/1‑93 (MQ=255)

                                                                                      ggcaa‑g‑gcaaAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCt   >  1:3632550/1‑93 (MQ=255)

                                                                                       gCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCtt  >  1:4529314/1‑93 (MQ=255)

                                                                                       gCAA‑G‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCtt  <  1:1706288/93‑1 (MQ=255)

GAATTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAA‑G‑G‑AAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTT  >  NC_000913/3430361‑3430539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GAATTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGG‑AAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAG  >  NC_000913/3430361‑3430546

GAATTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGG‑C                                                                                              >  SRR4302535.1621160/1‑94 (MQ=60)

GAATTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGG‑C                                                                                              <  SRR4302535.3342055/94‑1 (MQ=60)

 AATTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGG‑CA                                                                                             >  SRR4302535.1640081/1‑94 (MQ=60)

  ATTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGG‑CAA                                                                                            >  SRR4302535.1087686/1‑94 (MQ=60)

  ATTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGG‑CAA                                                                                            >  SRR4302535.2434301/1‑94 (MQ=60)

   TTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGG‑CAAA                                                                                           >  SRR4302535.3177870/1‑94 (MQ=60)

    TTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGG‑CAAAA                                                                                          >  SRR4302535.2538688/1‑94 (MQ=60)

     TCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAA                                                                                          >  SRR4302535.3617452/1‑94 (MQ=60)

      CTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAAC                                                                                         >  SRR4302535.3175717/1‑94 (MQ=60)

      CTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAAC                                                                                         <  SRR4302535.526402/94‑1 (MQ=60)

          CGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCT                                                                                     <  SRR4302535.552784/94‑1 (MQ=60)

          CGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCT                                                                                     <  SRR4302535.752748/94‑1 (MQ=60)

          CGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCT                                                                                     <  SRR4302535.2502718/94‑1 (MQ=60)

           GGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTC                                                                                    >  SRR4302535.193475/1‑94 (MQ=60)

           GGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTC                                                                                    >  SRR4302535.341645/1‑94 (MQ=60)

           GGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTC                                                                                    <  SRR4302535.361447/94‑1 (MQ=60)

           GGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTC                                                                                    >  SRR4302535.9249/1‑94 (MQ=60)

           GGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTC                                                                                    <  SRR4302535.1475010/94‑1 (MQ=60)

            GTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCG                                                                                   >  SRR4302535.1430058/1‑94 (MQ=60)

            GTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCG                                                                                   <  SRR4302535.1017664/94‑1 (MQ=60)

            GTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCG                                                                                   <  SRR4302535.2066387/94‑1 (MQ=60)

                 GTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCT                                                                              >  SRR4302535.1375894/1‑94 (MQ=60)

                 GTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCT                                                                              >  SRR4302535.105500/1‑94 (MQ=60)

                   CACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGC                                                                            >  SRR4302535.2688006/1‑94 (MQ=60)

                   CACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGC                                                                            >  SRR4302535.3256894/1‑94 (MQ=60)

                   CACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGC                                                                            >  SRR4302535.546171/1‑94 (MQ=60)

                   CACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGACCTTCGCTTCCCGGATTCCCTCTTGGCCCTGGCAAGGCCAAAACTTCTCCCCGCTGC                                                                            >  SRR4302535.2780436/1‑94 (MQ=60)

                     CAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTC                                                                          <  SRR4302535.1641676/94‑1 (MQ=60)

                      AAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCA                                                                         <  SRR4302535.3692298/94‑1 (MQ=60)

                      AAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCA                                                                         >  SRR4302535.4536445/1‑94 (MQ=60)

                       AGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAA                                                                        >  SRR4302535.1811030/1‑94 (MQ=60)

                         AATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGC                                                                      <  SRR4302535.3795573/94‑1 (MQ=60)

                          ATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCG                                                                     <  SRR4302535.4500663/94‑1 (MQ=60)

                           TCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGC                                                                    <  SRR4302535.1732854/94‑1 (MQ=60)

                            CGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCA                                                                   <  SRR4302535.67210/94‑1 (MQ=60)

                             GCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCAC                                                                  <  SRR4302535.2034673/94‑1 (MQ=60)

                             GCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCAC                                                                  <  SRR4302535.1484769/94‑1 (MQ=60)

                              CACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACA                                                                 >  SRR4302535.1823741/1‑94 (MQ=60)

                              CACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACA                                                                 >  SRR4302535.2574360/1‑94 (MQ=60)

                               ACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAA                                                                >  SRR4302535.4476138/1‑94 (MQ=60)

                               ACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAA                                                                >  SRR4302535.2491547/1‑94 (MQ=60)

                               ACCTTGTTTTATTAACCAGTCAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAACAACTTCTCGCCCCTGCTCAAGCGCACAA                                                                >  SRR4302535.2875299/1‑94 (MQ=60)

                                 CTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACG                                                              >  SRR4302535.4170079/1‑94 (MQ=60)

                                 CTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACG                                                              >  SRR4302535.2750283/1‑94 (MQ=60)

                                 CTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACG                                                              >  SRR4302535.3076611/1‑94 (MQ=60)

                                   TGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTG                                                            >  SRR4302535.2216243/1‑94 (MQ=60)

                                      TTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTG                                                         >  SRR4302535.4287618/1‑94 (MQ=60)

                                      TTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTG                                                         >  SRR4302535.3305412/1‑94 (MQ=60)

                                           TAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACA                                                    >  SRR4302535.3749182/1‑94 (MQ=60)

                                             ACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTTCTGTCACCAG                                                  <  SRR4302535.2749685/94‑1 (MQ=60)

                                              CCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGC                                                 <  SRR4302535.3453652/94‑1 (MQ=60)

                                              CCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGC                                                 >  SRR4302535.2149120/1‑94 (MQ=60)

                                               CAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCG                                                >  SRR4302535.864047/1‑94 (MQ=60)

                                                AGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGA                                               >  SRR4302535.653051/1‑94 (MQ=60)

                                                AGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGA                                               >  SRR4302535.174875/1‑94 (MQ=60)

                                                 GTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAA                                              >  SRR4302535.1722852/1‑94 (MQ=60)

                                                 GTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAA                                              >  SRR4302535.241710/1‑94 (MQ=60)

                                                  TGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAAC                                             <  SRR4302535.4002833/94‑1 (MQ=60)

                                                   GAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCACAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACC                                            >  SRR4302535.1701110/1‑94 (MQ=60)

                                                   GAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGCCACCAGCGAACC                                            >  SRR4302535.1483960/1‑94 (MQ=60)

                                                     GGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCAC                                          <  SRR4302535.1966010/94‑1 (MQ=60)

                                                      GCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACT                                         >  SRR4302535.289937/1‑94 (MQ=60)

                                                      GCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACT                                         >  SRR4302535.1684649/1‑94 (MQ=60)

                                                      GCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACT                                         <  SRR4302535.3548531/94‑1 (MQ=60)

                                                        CCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTAC                                       >  SRR4302535.1334404/1‑94 (MQ=60)

                                                        CCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTAC                                       >  SRR4302535.2622425/1‑94 (MQ=60)

                                                        CCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTAC                                       >  SRR4302535.737890/1‑94 (MQ=60)

                                                        CCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTAC                                       >  SRR4302535.586588/1‑94 (MQ=60)

                                                         CTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACG                                      >  SRR4302535.668852/1‑94 (MQ=60)

                                                         CTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACG                                      >  SRR4302535.4198442/1‑94 (MQ=60)

                                                          TTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGC                                     >  SRR4302535.3963953/1‑94 (MQ=60)

                                                           TCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGCACCACTACGCA                                    >  SRR4302535.604912/1‑94 (MQ=60)

                                                             GCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAA                                  >  SRR4302535.2744402/1‑94 (MQ=60)

                                                              CTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTTCTGTCACCAGCGAACCACTACGCAAAG                                 <  SRR4302535.3515896/94‑1 (MQ=60)

                                                              CTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAG                                 <  SRR4302535.3540353/94‑1 (MQ=60)

                                                              CTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAG                                 <  SRR4302535.3741184/94‑1 (MQ=60)

                                                              CTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAG                                 >  SRR4302535.3633840/1‑94 (MQ=60)

                                                              CTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAG                                 <  SRR4302535.2424013/94‑1 (MQ=60)

                                                              CTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAG                                 >  SRR4302535.710108/1‑94 (MQ=60)

                                                                TCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCC                               <  SRR4302535.461396/94‑1 (MQ=60)

                                                                TCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCC                               <  SRR4302535.2676843/94‑1 (MQ=60)

                                                                TCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCC                               >  SRR4302535.1274425/1‑94 (MQ=60)

                                                                    GGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTT                           >  SRR4302535.1030823/1‑94 (MQ=60)

                                                                    GGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTT                           <  SRR4302535.2700308/94‑1 (MQ=60)

                                                                     GATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTC                          >  SRR4302535.859004/1‑94 (MQ=60)

                                                                      ATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCC                         >  SRR4302535.312750/1‑94 (MQ=60)

                                                                       TTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTAGGCAAAGCCGCTTCCC                        >  SRR4302535.4173722/1‑94 (MQ=60)

                                                                        TCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCAC                       <  SRR4302535.358877/94‑1 (MQ=60)

                                                                         CCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACC                      >  SRR4302535.1594271/1‑94 (MQ=60)

                                                                          CCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCA                     <  SRR4302535.1269452/94‑1 (MQ=60)

                                                                           CTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTTACCAGCGAACCACTACGCAAAGCCGCTTCCACCTC                    >  SRR4302535.872708/1‑94 (MQ=60)

                                                                           CTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCAC                    >  SRR4302535.3774806/1‑94 (MQ=60)

                                                                           CTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCAC                    >  SRR4302535.3426416/1‑94 (MQ=60)

                                                                           CTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCAC                    >  SRR4302535.508134/1‑94 (MQ=60)

                                                                            TATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACC                   <  SRR4302535.1477800/94‑1 (MQ=60)

                                                                                GGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTA               <  SRR4302535.2802183/94‑1 (MQ=60)

                                                                                 GACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTTCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTAC              <  SRR4302535.1209776/94‑1 (MQ=60)

                                                                                 GACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTAC              <  SRR4302535.1632952/94‑1 (MQ=60)

                                                                                   CCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACAC            >  SRR4302535.513303/1‑94 (MQ=60)

                                                                                   CCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACAC            <  SRR4302535.4054889/94‑1 (MQ=60)

                                                                                   CCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACAC            >  SRR4302535.602459/1‑94 (MQ=60)

                                                                                   CCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACAC            >  SRR4302535.649455/1‑94 (MQ=60)

                                                                                     TGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCT          <  SRR4302535.2560553/94‑1 (MQ=60)

                                                                                     TGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCT          >  SRR4302535.70733/1‑94 (MQ=60)

                                                                                     TGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCT          >  SRR4302535.3657726/1‑94 (MQ=60)

                                                                                     TGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCT          <  SRR4302535.3508157/94‑1 (MQ=60)

                                                                                      GGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTT         >  SRR4302535.4560326/1‑94 (MQ=60)

                                                                                      GGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTT         >  SRR4302535.1216737/1‑94 (MQ=60)

                                                                                       GCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTT        <  SRR4302535.1718622/94‑1 (MQ=60)

                                                                                       GCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTT        >  SRR4302535.2404275/1‑94 (MQ=60)

                                                                                        CAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTA       <  SRR4302535.4323761/94‑1 (MQ=60)

                                                                                         AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTAC      <  SRR4302535.4568517/94‑1 (MQ=60)

                                                                                         AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTAC      >  SRR4302535.2418246/1‑94 (MQ=60)

                                                                                         AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTAC      >  SRR4302535.518947/1‑94 (MQ=60)

                                                                                          AGGCAAAACCTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACT     <  SRR4302535.2613878/94‑1 (MQ=60)

                                                                                          AGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACT     >  SRR4302535.3781522/1‑94 (MQ=60)

                                                                                          AGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACT     <  SRR4302535.2087103/94‑1 (MQ=60)

                                                                                            C‑AAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAG  <  SRR4302535.12188/94‑1 (MQ=60)

                                                                                            C‑AAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAG  >  SRR4302535.2631974/1‑94 (MQ=60)

GAATTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGG‑AAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAG  >  NC_000913/3430361‑3430546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`