Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,431,203 T→C intergenic (‑6/‑124) smf ← / → def putative Rossmann‑fold nucleotide‑binding protein/peptide deformylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,431,2030TC99.5% 682.7 / NA 214intergenic (‑6/‑124)smf/defputative Rossmann‑fold nucleotide‑binding protein/peptide deformylase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (116/97);  total (116/98)
Fisher's exact test for biased strand distribution p-value = 4.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 235 total aligned reads displayed.

ACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATA  >  NC_000913/3431115‑3431291
                                                                                        |                                                                                        
aCCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTcc                                                                                      >  1:4372639/1‑93 (MQ=255)
 cccAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTccc                                                                                     <  1:3699858/93‑1 (MQ=255)
  ccAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCt                                                                                    >  2:3389316/1‑93 (MQ=255)
   cAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTg                                                                                   <  1:2002318/93‑1 (MQ=255)
      tGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCa                                                                                <  2:866982/93‑1 (MQ=255)
         gcaaTACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATAAAACGCAGCAAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATaa                                                                             <  2:3257160/90‑1 (MQ=255)
         gCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATaa                                                                             >  1:3145189/1‑93 (MQ=255)
           gATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAagc                                                                           <  2:3064438/93‑1 (MQ=255)
           gATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAagc                                                                           <  1:2847824/93‑1 (MQ=255)
           gATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAagc                                                                           >  2:4448868/1‑93 (MQ=255)
            aTACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAagca                                                                          >  2:638890/1‑93 (MQ=255)
             tACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAagcag                                                                         >  2:809966/1‑93 (MQ=255)
             tACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAagcag                                                                         >  1:937996/1‑93 (MQ=255)
             tACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAagcag                                                                         <  2:4324459/93‑1 (MQ=255)
              aCGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAagcagc                                                                        <  2:1538233/93‑1 (MQ=255)
               cGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGcc                                                                       <  1:1545645/93‑1 (MQ=255)
                  aCCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTa                                                                    <  2:207164/93‑1 (MQ=255)
                   ccATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAg                                                                   >  2:706439/1‑93 (MQ=255)
                      tatCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCaa                                                                <  1:485808/93‑1 (MQ=255)
                        tcatcGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATc                                                              >  2:2520595/1‑93 (MQ=255)
                        tcatcGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATc                                                              >  2:4098630/1‑93 (MQ=255)
                        tcatcGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATc                                                              >  2:1130317/1‑93 (MQ=255)
                          atcGCCGTACAAGCTTCTGATTCGCATTAACCGCAGCCAAATTTCTGTATCGCCCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCtt                                                            <  2:3186235/93‑1 (MQ=255)
                          atcGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCtt                                                            <  1:3752668/93‑1 (MQ=255)
                            cGCCGTACAAGCTGCTGATACTCATTAACCGCGGCCAAATTTCTGTATGGACCATCCTTCCCTCCCTGCCATAAGCAGCCTTAGCAATCTTTg                                                          <  1:4037205/93‑1 (MQ=255)
                            cGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTg                                                          >  1:3997324/1‑93 (MQ=255)
                               cGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGGCTTAGCAATCTTTGCGa                                                       >  1:2263757/1‑93 (MQ=255)
                                gTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGAt                                                      >  2:2462298/1‑93 (MQ=255)
                                gTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGAt                                                      >  1:3784294/1‑93 (MQ=255)
                                 tACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTCTCGACCATCCTTATCCCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGAtt                                                     <  2:3513882/93‑1 (MQ=255)
                                 tACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGAtt                                                     >  2:163269/1‑93 (MQ=255)
                                 tACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGAtt                                                     <  1:2398091/93‑1 (MQ=255)
                                    aaGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGt                                                  <  1:875830/93‑1 (MQ=255)
                                    aaGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGt                                                  <  2:2147321/93‑1 (MQ=255)
                                     aGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTc                                                 >  1:3600024/1‑93 (MQ=255)
                                     aGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTc                                                 >  1:2460234/1‑93 (MQ=255)
                                      gctgctGATACTCATTAAACCCAGCGAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCa                                                <  2:1516219/93‑1 (MQ=255)
                                       ctgctaATACTCATTAAATGAAGCGAAATTTCTGTATCAACCTTCCTTCCCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAg                                               <  2:2248198/93‑1 (MQ=255)
                                        tgctgATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGt                                              >  1:2971723/1‑93 (MQ=255)
                                          ctgATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGa                                            <  1:2497487/93‑1 (MQ=255)
                                           tgATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGAt                                           >  1:1752687/1‑93 (MQ=255)
                                             ttACTCATTAACCGCAGCCAATTTTCTGTATCGCCCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGc                                         <  2:2318322/92‑1 (MQ=255)
                                             aTACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGc                                         <  2:3576719/93‑1 (MQ=255)
                                             aTACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGc                                         <  1:156344/93‑1 (MQ=255)
                                             aTACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGc                                         <  1:3196179/93‑1 (MQ=255)
                                              tACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCt                                        >  2:2816546/1‑93 (MQ=255)
                                              tACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCt                                        >  1:575198/1‑93 (MQ=255)
                                              tACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCt                                        >  1:3353358/1‑93 (MQ=255)
                                               aCTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTg                                       >  2:2006713/1‑93 (MQ=255)
                                                cTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGt                                      >  1:2779345/1‑93 (MQ=255)
                                                cTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGt                                      <  2:2397426/93‑1 (MQ=255)
                                                cTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGt                                      >  2:4269208/1‑93 (MQ=255)
                                                cTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGt                                      >  2:1481798/1‑93 (MQ=255)
                                                 tCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCACTCTTTGCGATTGGTCAGTGATGCTGTc                                     >  2:1187717/1‑93 (MQ=255)
                                                 tCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTc                                     >  1:1392201/1‑93 (MQ=255)
                                                 tCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTc                                     <  1:1002893/93‑1 (MQ=255)
                                                 tCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTc                                     >  2:1027010/1‑93 (MQ=255)
                                                 tCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTc                                     >  1:4447629/1‑93 (MQ=255)
                                                  cATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTCGCAATCTTTGCGATTGGTCAGTGATGCTGTCa                                    >  2:1710153/1‑93 (MQ=255)
                                                   aTTAAACGCAGCCCAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCaa                                   >  2:4042042/1‑93 (MQ=255)
                                                   aTTAAACGCAGCCAAATTTCTGTATCGACCATCCTTCCCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCaa                                   <  1:1849413/93‑1 (MQ=255)
                                                   aTTAAACGCAGCCAAATTTCTGTATCGACCATCCTTCCCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCaa                                   <  1:3731471/93‑1 (MQ=255)
                                                   aTTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCaa                                   >  2:2754173/1‑93 (MQ=255)
                                                    ttAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAAt                                  >  1:4145309/1‑93 (MQ=255)
                                                    ttAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAAt                                  >  1:1255023/1‑93 (MQ=255)
                                                    ttAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAAt                                  >  2:4211297/1‑93 (MQ=255)
                                                     tAAACGCAGCCAAATTTCTGTATCGACCATCCTTCCCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATc                                 <  1:3177125/93‑1 (MQ=255)
                                                     tAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATc                                 >  1:1812908/1‑93 (MQ=255)
                                                      aaaCGCAGCCAAATTTCTGTATCGACCATCCTTCCCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCa                                <  1:3244398/93‑1 (MQ=255)
                                                        aCGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCaga                              <  2:165249/93‑1 (MQ=255)
                                                         cGCACCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCagag                             <  1:3273935/93‑1 (MQ=255)
                                                           cAGCCAAATTTCTGTATCGACTATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATTTTTGCGATTGGTCAGTGATGCTGTCAATCAGAggg                           <  1:2149404/93‑1 (MQ=255)
                                                           cAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAggg                           >  1:122630/1‑93 (MQ=255)
                                                           cAGCCAAATTTCTGTATCGACCATCCTGCCCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAggg                           <  2:364634/93‑1 (MQ=255)
                                                            aGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAgggg                          >  1:3667416/1‑93 (MQ=255)
                                                             gCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAggggg                         <  2:2209809/93‑1 (MQ=255)
                                                               cAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGAt                       >  1:756112/1‑93 (MQ=255)
                                                                aaaTTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGAtt                      >  1:1072774/1‑93 (MQ=255)
                                                                aaaTTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGAtt                      >  1:301059/1‑93 (MQ=255)
                                                                 aaTTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGAttt                     >  1:1147725/1‑93 (MQ=255)
                                                                 aaTTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGAttt                     >  1:2597276/1‑93 (MQ=255)
                                                                   tttCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGt                   >  1:211700/1‑93 (MQ=255)
                                                                    ttCTGTATCGCCCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTc                  <  2:4354194/93‑1 (MQ=255)
                                                                    ttCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTc                  <  1:461310/93‑1 (MQ=255)
                                                                    ttCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTc                  >  2:3841443/1‑93 (MQ=255)
                                                                     tCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCt                 >  2:273441/1‑93 (MQ=255)
                                                                      ttGTATCGACCATCCTTCCCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCta                <  1:2905091/92‑1 (MQ=255)
                                                                      cTGTATCGACCTTCCTTACCACCCTGCCATAAGCATCATTAGCAATCTTTCCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCta                <  2:767003/93‑1 (MQ=255)
                                                                      cTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCta                <  2:994878/93‑1 (MQ=255)
                                                                      cTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCta                >  2:4482972/1‑93 (MQ=255)
                                                                        gTATCGACCATCCTTCCCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCtaga              <  1:1102226/93‑1 (MQ=255)
                                                                        gTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCtaga              <  1:4312414/93‑1 (MQ=255)
                                                                         tATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCtagaa             <  1:2372623/93‑1 (MQ=255)
                                                                           tCGACCACCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCtagaata           <  2:620729/93‑1 (MQ=255)
                                                                            cGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCtagaatag          <  2:781116/93‑1 (MQ=255)
                                                                                cATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATagaaga      >  2:2497852/1‑93 (MQ=255)
                                                                                  tCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGaaa    <  2:4217699/93‑1 (MQ=255)
                                                                                    cTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAaata  >  1:3913934/1‑93 (MQ=255)
                                                                                    cTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAaata  >  1:4079631/1‑93 (MQ=255)
                                                                                    cTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAaata  >  2:1847133/1‑93 (MQ=255)
                                                                                        |                                                                                        
ACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATA  >  NC_000913/3431115‑3431291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTT  >  NC_000913/3431110‑3431296
                                                                                             |                                                                                             
TTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTAC                                                                                               >  SRR4302535.900297/1‑94 (MQ=60)
TTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTAC                                                                                               <  SRR4302535.2886393/94‑1 (MQ=60)
 TGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACC                                                                                              >  SRR4302535.4694461/1‑94 (MQ=60)
 TGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACC                                                                                              >  SRR4302535.3092400/1‑94 (MQ=60)
  tcnnncnatTGAGCGATACGGANCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACCCAGCCAAATTTCTGTATCGACCATCCTTACCT                                                                                             <  SRR4302535.2208158/85‑1 (MQ=60)
    CACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCC                                                                                           >  SRR4302535.4402556/1‑94 (MQ=60)
    CACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCC                                                                                           >  SRR4302535.3727564/1‑94 (MQ=60)
     ACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTNTATCGACCATCCTTACCTCCC                                                                                          >  SRR4302535.1758933/1‑94 (MQ=60)
      CCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCT                                                                                         <  SRR4302535.985289/94‑1 (MQ=60)
      CCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCT                                                                                         <  SRR4302535.3725486/94‑1 (MQ=60)
      CCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCT                                                                                         >  SRR4302535.139445/1‑94 (MQ=60)
       CCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTG                                                                                        >  SRR4302535.2335692/1‑94 (MQ=60)
        CAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGC                                                                                       <  SRR4302535.2016748/94‑1 (MQ=60)
         AGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACTCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCC                                                                                      >  SRR4302535.2059771/1‑94 (MQ=60)
         AGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCC                                                                                      <  SRR4302535.186766/94‑1 (MQ=60)
         AGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCC                                                                                      >  SRR4302535.2881965/1‑94 (MQ=60)
           TGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCAT                                                                                    >  SRR4302535.2251178/1‑94 (MQ=60)
           TGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCAT                                                                                    <  SRR4302535.2162747/94‑1 (MQ=60)
           TGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCAT                                                                                    >  SRR4302535.892234/1‑94 (MQ=60)
            GAGCGATACGGACCATATCATCGCCTTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATA                                                                                   <  SRR4302535.357064/94‑1 (MQ=60)
             AGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAA                                                                                  >  SRR4302535.3167062/1‑94 (MQ=60)
              GCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAG                                                                                 <  SRR4302535.4237999/94‑1 (MQ=60)
                GATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCA                                                                               <  SRR4302535.2868007/94‑1 (MQ=60)
                 ATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAG                                                                              >  SRR4302535.944640/1‑94 (MQ=60)
                   ACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCC                                                                            >  SRR4302535.3672238/1‑94 (MQ=60)
                    CGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCT                                                                           <  SRR4302535.1556775/94‑1 (MQ=60)
                     GGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTT                                                                          >  SRR4302535.185669/1‑94 (MQ=60)
                       ACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAG                                                                        <  SRR4302535.695842/94‑1 (MQ=60)
                        CCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGC                                                                       >  SRR4302535.44215/1‑94 (MQ=60)
                         CATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCA                                                                      >  SRR4302535.1090968/1‑94 (MQ=60)
                           TATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAAT                                                                    >  SRR4302535.826090/1‑94 (MQ=60)
                           TATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAAT                                                                    <  SRR4302535.3779502/94‑1 (MQ=60)
                           TATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAAT                                                                    <  SRR4302535.489215/94‑1 (MQ=60)
                            ATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATC                                                                   >  SRR4302535.2841958/1‑94 (MQ=60)
                               ATCGCCGTCAAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTT                                                                <  SRR4302535.2780730/94‑1 (MQ=60)
                               ATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTT                                                                <  SRR4302535.3778638/94‑1 (MQ=60)
                                TCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTG                                                               >  SRR4302535.4024672/1‑94 (MQ=60)
                                 CGCCGTACAAGCTGCTGATACTCATTAACCGCGGCCAAATTTCTGTATGGACCATCCTTCCCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGC                                                              <  SRR4302535.4064841/94‑1 (MQ=60)
                                  GCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCG                                                             <  SRR4302535.347116/94‑1 (MQ=60)
                                  CCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCG                                                             <  SRR4302535.2458786/94‑1 (MQ=60)
                                   CCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGGCTTAGCAATCTTTGCGA                                                            >  SRR4302535.2279887/1‑94 (MQ=60)
                                   CCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGA                                                            >  SRR4302535.2461400/1‑94 (MQ=60)
                                    CGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGAT                                                           >  SRR4302535.3810403/1‑94 (MQ=60)
                                    CGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGAT                                                           >  SRR4302535.3703447/1‑94 (MQ=60)
                                    CGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGAT                                                           >  SRR4302535.4241905/1‑94 (MQ=60)
                                      TACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTG                                                         <  SRR4302535.2415334/94‑1 (MQ=60)
                                      TACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTG                                                         <  SRR4302535.2402399/94‑1 (MQ=60)
                                        CAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTCCCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGT                                                       <  SRR4302535.2869477/94‑1 (MQ=60)
                                         AAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTC                                                      >  SRR4302535.3625006/1‑94 (MQ=60)
                                         AAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTC                                                      >  SRR4302535.2477970/1‑94 (MQ=60)
                                         AAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTC                                                      <  SRR4302535.1564118/94‑1 (MQ=60)
                                         AAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTC                                                      <  SRR4302535.882035/94‑1 (MQ=60)
                                          AGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCA                                                     >  SRR4302535.404445/1‑94 (MQ=60)
                                            CTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGCCCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGT                                                   <  SRR4302535.2947256/94‑1 (MQ=60)
                                            CTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGT                                                   <  SRR4302535.1456826/94‑1 (MQ=60)
                                            CTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGT                                                   >  SRR4302535.2992583/1‑94 (MQ=60)
                                              GCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGA                                                 >  SRR4302535.3778473/1‑94 (MQ=60)
                                              GCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGA                                                 >  SRR4302535.4347577/1‑94 (MQ=60)
                                               CTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGAT                                                >  SRR4302535.1765353/1‑94 (MQ=60)
                                               CTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGAT                                                <  SRR4302535.302888/94‑1 (MQ=60)
                                               CTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGAT                                                <  SRR4302535.2515504/94‑1 (MQ=60)
                                                 GATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGC                                              >  SRR4302535.3293019/1‑94 (MQ=60)
                                                  ATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCT                                             <  SRR4302535.714917/94‑1 (MQ=60)
                                                  ATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCT                                             >  SRR4302535.579237/1‑94 (MQ=60)
                                                  ATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCT                                             <  SRR4302535.157450/94‑1 (MQ=60)
                                                  ATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCT                                             >  SRR4302535.3376712/1‑94 (MQ=60)
                                                  ATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCT                                             <  SRR4302535.3218297/94‑1 (MQ=60)
                                                   TACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTG                                            >  SRR4302535.525253/1‑94 (MQ=60)
                                                    ACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGT                                           >  SRR4302535.2799133/1‑94 (MQ=60)
                                                    ACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGT                                           <  SRR4302535.337219/94‑1 (MQ=60)
                                                     CTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTC                                          >  SRR4302535.4478127/1‑94 (MQ=60)
                                                     CTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTC                                          >  SRR4302535.1402139/1‑94 (MQ=60)
                                                     CTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTC                                          >  SRR4302535.1260128/1‑94 (MQ=60)
                                                      TCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCA                                         <  SRR4302535.1010014/94‑1 (MQ=60)
                                                        ATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTCCCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAAT                                       <  SRR4302535.1862742/94‑1 (MQ=60)
                                                        ATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTCCCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAAT                                       <  SRR4302535.3757309/94‑1 (MQ=60)
                                                        ATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAAT                                       >  SRR4302535.1263997/1‑94 (MQ=60)
                                                        ATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAAT                                       >  SRR4302535.4173694/1‑94 (MQ=60)
                                                        ATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAAT                                       >  SRR4302535.3916864/1‑94 (MQ=60)
                                                        ATGAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCCCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAAT                                       <  SRR4302535.252847/94‑1 (MQ=60)
                                                         TTAAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATC                                      >  SRR4302535.1826017/1‑94 (MQ=60)
                                                          TAAACGCAGCCAAATTTCTGTATCGACCATCCTTCCCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCA                                     <  SRR4302535.3199103/94‑1 (MQ=60)
                                                           AAACGCAGCCAAATTTCTGTATCGACCATCCTTCCCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAG                                    <  SRR4302535.3266933/94‑1 (MQ=60)
                                                           AAACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAG                                    >  SRR4302535.4531979/1‑94 (MQ=60)
                                                             ACGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAG                                  >  SRR4302535.2611332/1‑94 (MQ=60)
                                                              CGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGG                                 <  SRR4302535.322957/94‑1 (MQ=60)
                                                              CGCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGG                                 <  SRR4302535.4053755/94‑1 (MQ=60)
                                                              CGCACCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGG                                 <  SRR4302535.3296673/94‑1 (MQ=60)
                                                               GCAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGG                                >  SRR4302535.123492/1‑94 (MQ=60)
                                                                CAGCCAAATTTCTGTATCGACTATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATTTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGG                               <  SRR4302535.2164862/94‑1 (MQ=60)
                                                                CAGCCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGG                               >  SRR4302535.3692814/1‑94 (MQ=60)
                                                                 AGCCAAATTTCTGTATCGCCCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGG                              <  SRR4302535.3220927/94‑1 (MQ=60)
                                                                   CCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGAT                            <  SRR4302535.3153208/94‑1 (MQ=60)
                                                                   CCAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGAT                            >  SRR4302535.761436/1‑94 (MQ=60)
                                                                    CAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATT                           >  SRR4302535.303156/1‑94 (MQ=60)
                                                                    CAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATT                           >  SRR4302535.1080399/1‑94 (MQ=60)
                                                                    CAAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATT                           <  SRR4302535.3867902/94‑1 (MQ=60)
                                                                     AAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTT                          >  SRR4302535.1155903/1‑94 (MQ=60)
                                                                     AAATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTT                          >  SRR4302535.2615929/1‑94 (MQ=60)
                                                                     AAATTTCTGTATCGACCACCCTTCCCCCCCCGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTT                          <  SRR4302535.3075443/94‑1 (MQ=60)
                                                                      AATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTG                         >  SRR4302535.1372731/1‑94 (MQ=60)
                                                                       ATTTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGT                        >  SRR4302535.213155/1‑94 (MQ=60)
                                                                         TTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCT                      <  SRR4302535.464534/94‑1 (MQ=60)
                                                                         TTCTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCT                      <  SRR4302535.2274515/94‑1 (MQ=60)
                                                                           TTGTATCGACCATCCTTCCCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAG                    <  SRR4302535.2925626/94‑1 (MQ=60)
                                                                           CTGTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAG                    <  SRR4302535.4098489/94‑1 (MQ=60)
                                                                             GTATCGACCATCCTTCCCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAA                  <  SRR4302535.1110101/94‑1 (MQ=60)
                                                                             GTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAA                  <  SRR4302535.4341914/94‑1 (MQ=60)
                                                                             GTATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAA                  <  SRR4302535.4006614/94‑1 (MQ=60)
                                                                              TATCGCCCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAAT                 <  SRR4302535.3212657/94‑1 (MQ=60)
                                                                              TATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAAT                 <  SRR4302535.2389665/94‑1 (MQ=60)
                                                                               ATCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATA                >  SRR4302535.4137100/1‑94 (MQ=60)
                                                                                TCGACCATCCTTCCCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAG               <  SRR4302535.976358/94‑1 (MQ=60)
                                                                                TCGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAG               <  SRR4302535.1203431/94‑1 (MQ=60)
                                                                                 CGACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTGTAGAATAGA              >  SRR4302535.1824883/1‑94 (MQ=60)
                                                                                   ACCATCCTTCCCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAG            <  SRR4302535.1136502/94‑1 (MQ=60)
                                                                                   ACCATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAG            <  SRR4302535.1594050/94‑1 (MQ=60)
                                                                                    CCATCCTTCCCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGA           <  SRR4302535.2497370/94‑1 (MQ=60)
                                                                                     CATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAA          <  SRR4302535.4681302/94‑1 (MQ=60)
                                                                                     CATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAA          >  SRR4302535.1141442/1‑94 (MQ=60)
                                                                                     CATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAA          <  SRR4302535.190694/94‑1 (MQ=60)
                                                                                      ATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAA         >  SRR4302535.3192371/1‑94 (MQ=60)
                                                                                      ATCCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAA         >  SRR4302535.4507698/1‑94 (MQ=60)
                                                                                        CCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATA       >  SRR4302535.373461/1‑94 (MQ=60)
                                                                                        CCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATA       >  SRR4302535.3940722/1‑94 (MQ=60)
                                                                                        CCTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATA       >  SRR4302535.4107537/1‑94 (MQ=60)
                                                                                         CTTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAA      <  SRR4302535.1048578/94‑1 (MQ=60)
                                                                                          TTACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAAT     >  SRR4302535.2445787/1‑94 (MQ=60)
                                                                                            ACCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCT   >  SRR4302535.308941/1‑94 (MQ=60)
                                                                                             CCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTT  <  SRR4302535.911590/94‑1 (MQ=60)
                                                                                             CCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTT  >  SRR4302535.2952212/1‑94 (MQ=60)
                                                                                             |                                                                                             
TTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTT  >  NC_000913/3431110‑3431296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑