Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,434,055 A→G P404P (CCA→CCG)  sun → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,434,0550AG99.5% 623.9 / ‑4.8 198P404P (CCA→CCG) sunhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (106/90);  total (106/92)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.30e-01

Only 100 of 201 total aligned reads displayed.

CTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCAGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGG  >  NC_000913/3433965‑3434144
                                                                                          |                                                                                         
 tcttCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCggag                                                                                        <  2:147601/91‑1 (MQ=255)
    tCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCaa                                                                                     >  1:3840512/1‑93 (MQ=255)
     cGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAAc                                                                                    <  1:1494039/93‑1 (MQ=255)
        tgtTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCgg                                                                                 >  1:1000483/1‑93 (MQ=255)
            aCCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTaa                                                                             >  2:3196682/1‑93 (MQ=255)
              cGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAAc                                                                           <  2:609958/93‑1 (MQ=255)
               ggAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACa                                                                          >  1:1786121/1‑93 (MQ=255)
                gAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACaa                                                                         >  1:3025530/1‑93 (MQ=255)
                 aaGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACaaa                                                                        <  1:3968966/93‑1 (MQ=255)
                   gagaATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAAt                                                                      >  1:3411250/1‑93 (MQ=255)
                   gagaATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAAt                                                                      >  2:4605861/1‑93 (MQ=255)
                    agaATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATc                                                                     >  2:3043896/1‑93 (MQ=255)
                    agaATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATc                                                                     >  1:2865934/1‑93 (MQ=255)
                     gaATCGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCt                                                                    >  2:2257318/1‑93 (MQ=255)
                     gaATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCt                                                                    >  2:1650606/1‑93 (MQ=255)
                     gaATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCt                                                                    >  2:1761130/1‑93 (MQ=255)
                      aaTAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTa                                                                   <  1:3441757/93‑1 (MQ=255)
                       aTAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTAc                                                                  >  1:1758226/1‑93 (MQ=255)
                       aTAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTAc                                                                  >  2:390764/1‑93 (MQ=255)
                       aTAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTAc                                                                  >  1:3270986/1‑93 (MQ=255)
                        tAGCCTGCAGATTAAATCCTTTTTGCAGCGTACCGCTGATGCCGATCTTTGCGAAACAGGAACACCTGAGCAACCGGGTAAACAAAATCTAcc                                                                 <  2:3804614/93‑1 (MQ=255)
                        tAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTAcc                                                                 <  2:775782/93‑1 (MQ=255)
                         aGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCt                                                                <  1:4170980/93‑1 (MQ=255)
                            cTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGt                                                             <  2:4178348/93‑1 (MQ=255)
                             tGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATATACCTGGTg                                                            >  2:682325/1‑93 (MQ=255)
                                 gATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGa                                                        >  2:3140807/1‑93 (MQ=255)
                                  aTTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGaa                                                       >  1:1838153/1‑93 (MQ=255)
                                    tAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAagg                                                     >  2:561210/1‑92 (MQ=255)
                                    tAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAaga                                                     <  2:3019047/93‑1 (MQ=255)
                                     aaaGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAagag                                                    >  1:2693229/1‑93 (MQ=255)
                                      aaGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAgg                                                   <  1:3788667/93‑1 (MQ=255)
                                      aaGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAgg                                                   <  1:2549849/93‑1 (MQ=255)
                                       aGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAggg                                                  >  2:1252851/1‑93 (MQ=255)
                                       aGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAggg                                                  <  2:1422824/93‑1 (MQ=255)
                                        gCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGc                                                 >  1:4507220/1‑93 (MQ=255)
                                        gCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGc                                                 <  1:4285791/93‑1 (MQ=255)
                                          cTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGa                                               >  1:2414116/1‑93 (MQ=255)
                                          cTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGa                                               >  2:1919674/1‑93 (MQ=255)
                                           tttttGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGAc                                              >  2:1619749/1‑93 (MQ=255)
                                           tttttGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGAc                                              >  1:2505331/1‑93 (MQ=255)
                                              ttGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACACAATCTACCTGGTGCCGAAGAGGGCGACGGc                                           >  1:795810/1‑93 (MQ=255)
                                              ttGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGc                                           <  2:1669344/93‑1 (MQ=255)
                                               tGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGct                                          <  1:3348044/93‑1 (MQ=255)
                                               tGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGct                                          >  2:4104055/1‑93 (MQ=255)
                                                gCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGctt                                         >  2:1041038/1‑93 (MQ=255)
                                                gCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGctt                                         <  2:3236502/93‑1 (MQ=255)
                                                   aCGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGcttctt                                      >  1:3664008/1‑93 (MQ=255)
                                                       tccGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTAc                                  <  1:2966967/92‑1 (MQ=255)
                                                       aCCGCTGATGCCGAACTTTGCGAAACAGGAAGACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTAc                                  >  2:3756616/1‑93 (MQ=255)
                                                       aCCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTAc                                  <  1:4311367/93‑1 (MQ=255)
                                                        ccGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACAGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACg                                 >  1:3303399/1‑93 (MQ=255)
                                                         cGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACgc                                >  1:1068506/1‑93 (MQ=255)
                                                         cGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACgc                                >  2:196007/1‑93 (MQ=255)
                                                         cGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACgc                                <  1:2035739/93‑1 (MQ=255)
                                                         cGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACgc                                <  2:1328073/93‑1 (MQ=255)
                                                         cGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACgc                                <  1:4457133/93‑1 (MQ=255)
                                                            tGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACgctaa                             <  1:1708531/93‑1 (MQ=255)
                                                               tGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACgctaagct                          >  2:3615424/1‑93 (MQ=255)
                                                                gCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACgctaagcta                         <  1:3166611/93‑1 (MQ=255)
                                                                 ccGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACgctaagctaa                        >  2:4037736/1‑93 (MQ=255)
                                                                 ccGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACgctaagctaa                        <  1:1480440/93‑1 (MQ=255)
                                                                 ccGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACgctaagctaa                        >  1:4073617/1‑93 (MQ=255)
                                                                 ccGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACgctaagctaa                        <  1:857743/93‑1 (MQ=255)
                                                                  cGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAAt                       >  1:1633319/1‑93 (MQ=255)
                                                                   gAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATc                      <  2:2787133/93‑1 (MQ=255)
                                                                    aaCTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCa                     >  1:2547301/1‑93 (MQ=255)
                                                                    aaCTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCa                     >  2:2683347/1‑93 (MQ=255)
                                                                     aCTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCaa                    >  1:899050/1‑93 (MQ=255)
                                                                     aCTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCaa                    >  2:3254804/1‑93 (MQ=255)
                                                                     aCTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCaa                    >  1:1672937/1‑93 (MQ=255)
                                                                     aCTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCaa                    >  2:1902532/1‑93 (MQ=255)
                                                                      cTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCaaa                   >  1:3081595/1‑93 (MQ=255)
                                                                       tttGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCaaaa                  >  2:253532/1‑93 (MQ=255)
                                                                       tttGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCaaaa                  >  2:552345/1‑93 (MQ=255)
                                                                       tttGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCaaaa                  >  2:2094576/1‑93 (MQ=255)
                                                                        ttGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCaaaaa                 <  2:394833/93‑1 (MQ=255)
                                                                         tGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAg                <  1:2925621/93‑1 (MQ=255)
                                                                          gCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGt               <  1:1534963/93‑1 (MQ=255)
                                                                          gCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGt               <  2:2281558/93‑1 (MQ=255)
                                                                          gCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGt               <  1:1215120/93‑1 (MQ=255)
                                                                           cGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGtg              <  1:3886893/93‑1 (MQ=255)
                                                                            gAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGtga             <  2:3299028/93‑1 (MQ=255)
                                                                            gAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGtga             >  2:4358899/1‑93 (MQ=255)
                                                                             aaaCAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGtgat            <  1:3494981/93‑1 (MQ=255)
                                                                              aaCAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGtgatg           <  1:2229556/93‑1 (MQ=255)
                                                                              aaCAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGtgatg           >  2:4645982/1‑93 (MQ=255)
                                                                              aaCAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGtgatg           <  2:2598946/93‑1 (MQ=255)
                                                                               aCAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGtgatga          >  2:3398641/1‑93 (MQ=255)
                                                                               aCAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGtgatga          <  2:1007485/93‑1 (MQ=255)
                                                                                 aGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATgaga        <  1:2515991/93‑1 (MQ=255)
                                                                                  ggAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGAt       <  1:966802/93‑1 (MQ=255)
                                                                                  ggAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGAt       <  1:2131294/93‑1 (MQ=255)
                                                                                  ggAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGAt       <  1:764752/93‑1 (MQ=255)
                                                                                  ggAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGAt       <  1:1811683/93‑1 (MQ=255)
                                                                                     acacCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAAc    >  1:577877/1‑93 (MQ=255)
                                                                                     acacCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAAc    >  1:1118897/1‑93 (MQ=255)
                                                                                      cacCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACg   <  1:1976013/93‑1 (MQ=255)
                                                                                       acCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACgg  <  2:4462347/93‑1 (MQ=255)
                                                                                       acCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACgg  >  2:3987561/1‑93 (MQ=255)
                                                                                       acCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACgg  <  1:2811365/93‑1 (MQ=255)
                                                                                          |                                                                                         
CTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCAGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGG  >  NC_000913/3433965‑3434144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCAGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCG  >  NC_000913/3433963‑3434148
                                                                                            |                                                                                             
ACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGG                                                                                              >  SRR4302535.778008/1‑94 (MQ=60)
 CCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGA                                                                                             <  SRR4302535.4659085/94‑1 (MQ=60)
     TTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAA                                                                                         >  SRR4302535.3866976/1‑94 (MQ=60)
     TTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAA                                                                                         <  SRR4302535.3737251/94‑1 (MQ=60)
       CGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACC                                                                                       >  SRR4302535.4084445/1‑94 (MQ=60)
       CGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACC                                                                                       <  SRR4302535.1504778/94‑1 (MQ=60)
       CGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACC                                                                                       <  SRR4302535.2319818/94‑1 (MQ=60)
         GTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGG                                                                                     >  SRR4302535.1007594/1‑94 (MQ=60)
            TTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTA                                                                                  <  SRR4302535.2561427/94‑1 (MQ=60)
             TACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAA                                                                                 >  SRR4302535.3817278/1‑94 (MQ=60)
                CGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACA                                                                              >  SRR4302535.1143445/1‑94 (MQ=60)
                CGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACA                                                                              >  SRR4302535.924082/1‑94 (MQ=60)
                CGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACA                                                                              >  SRR4302535.4293151/1‑94 (MQ=60)
                CGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACA                                                                              >  SRR4302535.1799046/1‑94 (MQ=60)
                 GGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAA                                                                             >  SRR4302535.3046710/1‑94 (MQ=60)
                   AAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAA                                                                           <  SRR4302535.3996074/94‑1 (MQ=60)
                    AGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAAT                                                                          >  SRR4302535.3434981/1‑94 (MQ=60)
                     GAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATC                                                                         >  SRR4302535.2886235/1‑94 (MQ=60)
                      AGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCT                                                                        >  SRR4302535.548308/1‑94 (MQ=60)
                        AATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTAC                                                                      >  SRR4302535.1770935/1‑94 (MQ=60)
                        AATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTAC                                                                      <  SRR4302535.3465692/94‑1 (MQ=60)
                        AATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTAC                                                                      >  SRR4302535.3293707/1‑94 (MQ=60)
                          TAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCT                                                                    <  SRR4302535.3895635/94‑1 (MQ=60)
                           AGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTG                                                                   >  SRR4302535.889256/1‑94 (MQ=60)
                           AGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTG                                                                   <  SRR4302535.4199538/94‑1 (MQ=60)
                            GCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGG                                                                  <  SRR4302535.1652581/94‑1 (MQ=60)
                            GCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGG                                                                  <  SRR4302535.1103175/94‑1 (MQ=60)
                            GCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGG                                                                  <  SRR4302535.1006727/94‑1 (MQ=60)
                             CCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAAGTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGT                                                                 >  SRR4302535.3325549/1‑94 (MQ=60)
                             CCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGT                                                                 >  SRR4302535.3468301/1‑94 (MQ=60)
                             CCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGT                                                                 >  SRR4302535.2961455/1‑94 (MQ=60)
                              CTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTG                                                                >  SRR4302535.1830710/1‑94 (MQ=60)
                                  AGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGA                                                            >  SRR4302535.1015896/1‑94 (MQ=60)
                                  AGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGA                                                            >  SRR4302535.2812683/1‑94 (MQ=60)
                                   GATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAA                                                           >  SRR4302535.1851413/1‑94 (MQ=60)
                                   GATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCAGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAA                                                           <  SRR4302535.3628110/94‑1 (MQ=60)
                                    ATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAG                                                          <  SRR4302535.2157840/94‑1 (MQ=60)
                                      TAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAG                                                        >  SRR4302535.2712476/1‑94 (MQ=60)
                                       AAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGG                                                       <  SRR4302535.2859134/94‑1 (MQ=60)
                                        AAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGG                                                      <  SRR4302535.2568204/94‑1 (MQ=60)
                                        AAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGG                                                      <  SRR4302535.2568205/94‑1 (MQ=60)
                                        AAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGG                                                      <  SRR4302535.3814798/94‑1 (MQ=60)
                                         AGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGC                                                     >  SRR4302535.4538154/1‑94 (MQ=60)
                                          GCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCG                                                    <  SRR4302535.4315111/94‑1 (MQ=60)
                                          GCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCG                                                    <  SRR4302535.3197674/94‑1 (MQ=60)
                                           CCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGA                                                   >  SRR4302535.2431494/1‑94 (MQ=60)
                                            CTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGAC                                                  >  SRR4302535.1800692/1‑94 (MQ=60)
                                            CTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGAC                                                  >  SRR4302535.2523392/1‑94 (MQ=60)
                                             TTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACG                                                 >  SRR4302535.2942668/1‑94 (MQ=60)
                                             TTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACG                                                 >  SRR4302535.2005127/1‑94 (MQ=60)
                                               TTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACACAATCTACCTGGTGCCGAAGAGGGCGACGGC                                               >  SRR4302535.801396/1‑94 (MQ=60)
                                                TTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCT                                              <  SRR4302535.413555/94‑1 (MQ=60)
                                                 TGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTT                                             <  SRR4302535.3371367/94‑1 (MQ=60)
                                                   CAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCT                                           <  SRR4302535.2708751/94‑1 (MQ=60)
                                                   CAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCT                                           >  SRR4302535.2079481/1‑94 (MQ=60)
                                                    AACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTT                                          >  SRR4302535.3689372/1‑94 (MQ=60)
                                                     ACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTT                                         <  SRR4302535.4522741/94‑1 (MQ=60)
                                                     ACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTT                                         >  SRR4302535.3411160/1‑94 (MQ=60)
                                                      CGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTT                                        <  SRR4302535.3156621/94‑1 (MQ=60)
                                                        TACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTAC                                      <  SRR4302535.1942834/94‑1 (MQ=60)
                                                         TCCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACG                                     <  SRR4302535.2987783/94‑1 (MQ=60)
                                                         ACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACG                                     <  SRR4302535.4340860/94‑1 (MQ=60)
                                                         ACCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACAGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACG                                     >  SRR4302535.3326372/1‑94 (MQ=60)
                                                          CCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGC                                    >  SRR4302535.1076103/1‑94 (MQ=60)
                                                          CCGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGC                                    >  SRR4302535.3019191/1‑94 (MQ=60)
                                                           CGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCT                                   <  SRR4302535.4487700/94‑1 (MQ=60)
                                                           CGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCT                                   >  SRR4302535.1707937/1‑94 (MQ=60)
                                                           CGCTGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCT                                   <  SRR4302535.2050403/94‑1 (MQ=60)
                                                              TGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAG                                <  SRR4302535.1720884/94‑1 (MQ=60)
                                                              TGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAG                                >  SRR4302535.1748729/1‑94 (MQ=60)
                                                              TGATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAG                                <  SRR4302535.3463106/94‑1 (MQ=60)
                                                               GATGCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGC                               <  SRR4302535.2532122/94‑1 (MQ=60)
                                                                  GCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAA                            <  SRR4302535.3188555/94‑1 (MQ=60)
                                                                  GCCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAA                            >  SRR4302535.4101491/1‑94 (MQ=60)
                                                                   CCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAAT                           <  SRR4302535.1491029/94‑1 (MQ=60)
                                                                   CCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAAT                           >  SRR4302535.4420132/1‑94 (MQ=60)
                                                                   CCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAAT                           >  SRR4302535.1645124/1‑94 (MQ=60)
                                                                   CCGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAAT                           <  SRR4302535.863789/94‑1 (MQ=60)
                                                                    CGAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATC                          <  SRR4302535.1497866/94‑1 (MQ=60)
                                                                     GAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCA                         >  SRR4302535.1303197/1‑94 (MQ=60)
                                                                     GAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCA                         >  SRR4302535.2565631/1‑94 (MQ=60)
                                                                     GAACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCA                         <  SRR4302535.3939984/94‑1 (MQ=60)
                                                                      AACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAA                        <  SRR4302535.431699/94‑1 (MQ=60)
                                                                      AACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAA                        >  SRR4302535.1685044/1‑94 (MQ=60)
                                                                      AACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAA                        >  SRR4302535.3364355/1‑94 (MQ=60)
                                                                      AACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAA                        >  SRR4302535.905419/1‑94 (MQ=60)
                                                                       ACTTTGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAA                       >  SRR4302535.3103074/1‑94 (MQ=60)
                                                                           TGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGT                   <  SRR4302535.2946245/94‑1 (MQ=60)
                                                                           TGCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGT                   <  SRR4302535.2446165/94‑1 (MQ=60)
                                                                            GCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTG                  <  SRR4302535.1223835/94‑1 (MQ=60)
                                                                            GCGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTG                  <  SRR4302535.1546021/94‑1 (MQ=60)
                                                                             CGAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGA                 <  SRR4302535.3913554/94‑1 (MQ=60)
                                                                              GAAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGAT                <  SRR4302535.641152/94‑1 (MQ=60)
                                                                               AAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATG               <  SRR4302535.3093811/94‑1 (MQ=60)
                                                                               AAACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATG               <  SRR4302535.3519261/94‑1 (MQ=60)
                                                                                AACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGA              >  SRR4302535.1542196/1‑94 (MQ=60)
                                                                                AACAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGA              <  SRR4302535.2245411/94‑1 (MQ=60)
                                                                                  CAGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGA            >  SRR4302535.776043/1‑94 (MQ=60)
                                                                                   AGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGAT           <  SRR4302535.2534127/94‑1 (MQ=60)
                                                                                   AGGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAAACAAAAAGTGATGAGAT           >  SRR4302535.1595247/1‑94 (MQ=60)
                                                                                    GGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATA          <  SRR4302535.770140/94‑1 (MQ=60)
                                                                                    GGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATA          <  SRR4302535.973671/94‑1 (MQ=60)
                                                                                    GGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATA          <  SRR4302535.1824782/94‑1 (MQ=60)
                                                                                    GGAACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATA          <  SRR4302535.2146658/94‑1 (MQ=60)
                                                                                      AACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAAC        >  SRR4302535.581935/1‑94 (MQ=60)
                                                                                      AACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAAC        >  SRR4302535.1126879/1‑94 (MQ=60)
                                                                                       ACACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACG       >  SRR4302535.85976/1‑94 (MQ=60)
                                                                                        CACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGG      >  SRR4302535.1784835/1‑94 (MQ=60)
                                                                                        CACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGG      <  SRR4302535.1990243/94‑1 (MQ=60)
                                                                                         ACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGG     <  SRR4302535.2831349/94‑1 (MQ=60)
                                                                                         ACCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGG     >  SRR4302535.849956/1‑94 (MQ=60)
                                                                                          CCGGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGT    <  SRR4302535.3254633/94‑1 (MQ=60)
                                                                                            GGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCG  <  SRR4302535.1707315/94‑1 (MQ=60)
                                                                                            |                                                                                             
ACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCAGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCG  >  NC_000913/3433963‑3434148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑