Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,450,109 G→A P152L (CCG→CTG)  rplC ← 50S ribosomal subunit protein L3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,450,1090GA100.0% 851.8 / NA 232P152L (CCG→CTG) rplC50S ribosomal subunit protein L3
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (122/110);  total (122/110)

Only 100 of 239 total aligned reads displayed.

GCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTA  >  NC_000913/3450019‑3450198
                                                                                          |                                                                                         
gCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGa                                                                                         >  1:1162291/1‑93 (MQ=255)
gCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGa                                                                                         >  1:990576/1‑93 (MQ=255)
  gTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGt                                                                                       <  2:1717892/93‑1 (MQ=255)
   tCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTc                                                                                      >  1:3546053/1‑93 (MQ=255)
    cAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCt                                                                                     >  1:580455/1‑93 (MQ=255)
    cAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCt                                                                                     <  1:625660/93‑1 (MQ=255)
     aaCGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTg                                                                                    <  2:3475696/93‑1 (MQ=255)
      aCGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTgg                                                                                   >  1:2791506/1‑93 (MQ=255)
         cgTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTc                                                                                <  2:3126179/93‑1 (MQ=255)
           tactacGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTg                                                                              >  1:5909/1‑93 (MQ=255)
           tactacGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTg                                                                              >  1:2986985/1‑93 (MQ=255)
                  tCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATa                                                                       >  2:3112391/1‑93 (MQ=255)
                  tCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATa                                                                       <  2:3209088/93‑1 (MQ=255)
                  tCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATa                                                                       <  1:1051825/93‑1 (MQ=255)
                  tCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATa                                                                       <  1:3369896/93‑1 (MQ=255)
                  ccAAGGCTCTGATCGGTTACACGTTCGTTCCCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATa                                                                       <  1:3705315/92‑1 (MQ=255)
                   cAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAg                                                                      >  1:3637116/1‑93 (MQ=255)
                    aaGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGa                                                                     >  1:1237245/1‑93 (MQ=255)
                     aGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGaa                                                                    >  2:791357/1‑93 (MQ=255)
                     aGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGaa                                                                    >  1:4219049/1‑93 (MQ=255)
                     aGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGaa                                                                    <  1:2670911/93‑1 (MQ=255)
                      ggCTCTGAACGGTTACACGTTCGGTCCCCATCTGACCTGCCGTTTTCTTGCCTGGGAACACGTAGCCCAGAGTCTGGTTCTGACCGATAGagc                                                                   <  2:937731/93‑3 (MQ=255)
                       gCTCTGAACGGTTACACGTTCGTTCCCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAAcc                                                                  <  1:1830652/93‑1 (MQ=255)
                       gCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAAcc                                                                  >  2:2430341/1‑93 (MQ=255)
                         tctGACCGGTTCCACGTTCGTTACCCAGCTGACCTGCCATTTTCTTGCCTTTGAACACTATGCCCAGAGTCTGGTTCTGACCGATAGAACCCg                                                                <  1:1552577/93‑1 (MQ=255)
                           tGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGa                                                              <  2:3068820/93‑1 (MQ=255)
                           tGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGa                                                              >  2:3960973/1‑93 (MQ=255)
                            gAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGaa                                                             <  2:3370682/93‑1 (MQ=255)
                             aaCGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAAc                                                            <  1:377260/93‑1 (MQ=255)
                             aaCGGTTACACGTGCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAAc                                                            <  1:2134755/93‑1 (MQ=255)
                              aCGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAAcg                                                           >  1:239215/1‑93 (MQ=255)
                               cGGTTACACGTTCGTTACCCATCTGCCCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAAcgc                                                          <  1:1454972/93‑1 (MQ=255)
                                 gTTACACGTTCGTTACCCATCTGACCTACCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCgg                                                        <  1:176652/93‑1 (MQ=255)
                                   tACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTg                                                      <  2:1067256/93‑1 (MQ=255)
                                    acacGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTga                                                     <  2:849007/93‑1 (MQ=255)
                                    acacGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTga                                                     >  1:3219604/1‑93 (MQ=255)
                                     cacGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTgag                                                    <  2:3338248/93‑1 (MQ=255)
                                      acGTTCGTTACCCATCTGATCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTgaga                                                   >  2:4562253/1‑93 (MQ=255)
                                      acGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCCGACCGATAGAACCCGGAACGCGGTgaga                                                   >  1:4590581/1‑93 (MQ=255)
                                       cgttcgttACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGAc                                                  >  2:402369/1‑93 (MQ=255)
                                        gttcgttACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACa                                                 >  1:2890028/1‑93 (MQ=255)
                                         ttcgttACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACaa                                                <  2:4001559/93‑1 (MQ=255)
                                            gttACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGa                                             <  1:3392211/93‑1 (MQ=255)
                                             ttACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGGACGCGGTGAGACAAGGAg                                            >  2:4171909/1‑93 (MQ=255)
                                              tACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGt                                           >  1:538458/1‑93 (MQ=255)
                                               aCCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGtt                                          <  2:4332229/93‑1 (MQ=255)
                                                cccATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTa                                         >  1:1929462/1‑93 (MQ=255)
                                                 ccATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTAc                                        >  1:2060253/1‑93 (MQ=255)
                                                 ccACCTGCCCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTAc                                        <  2:4124373/93‑1 (MQ=255)
                                                   aTCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCg                                      <  2:3090601/93‑1 (MQ=255)
                                                    tCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCgg                                     >  2:660249/1‑92 (MQ=255)
                                                    tCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGt                                     <  1:857775/93‑1 (MQ=255)
                                                    tCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGt                                     >  1:2496051/1‑93 (MQ=255)
                                                      tGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGa                                   >  1:4508437/1‑93 (MQ=255)
                                                      tGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGa                                   <  2:1648979/93‑1 (MQ=255)
                                                        aCCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGt                                 <  1:1939159/93‑1 (MQ=255)
                                                        aCCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGt                                 >  2:1816280/1‑93 (MQ=255)
                                                        aCCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGt                                 <  2:1296895/93‑1 (MQ=255)
                                                         ccTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTa                                <  1:2845199/93‑1 (MQ=255)
                                                          cTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAg                               >  2:1491468/1‑93 (MQ=255)
                                                          cTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAg                               <  2:2464139/93‑1 (MQ=255)
                                                           tGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGc                              <  2:2073506/93‑1 (MQ=255)
                                                            gCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCg                             >  1:3101835/1‑93 (MQ=255)
                                                              cATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTc                           >  1:756616/1‑93 (MQ=255)
                                                               aTTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTcc                          <  2:9163/93‑1 (MQ=255)
                                                               aTTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTcc                          <  2:2635593/93‑1 (MQ=255)
                                                                ttttCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCt                         >  1:1642694/1‑93 (MQ=255)
                                                                ttttCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCt                         <  2:3700837/93‑1 (MQ=255)
                                                                 tttCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTg                        <  1:4328293/93‑1 (MQ=255)
                                                                 tttCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTg                        >  2:3266571/1‑93 (MQ=255)
                                                                 tttCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTg                        <  1:4368143/93‑1 (MQ=255)
                                                                  ttCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTgg                       <  1:3567671/93‑1 (MQ=255)
                                                                  ttCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTgg                       <  2:1306030/93‑1 (MQ=255)
                                                                  ttCTTGCCTTCGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTgg                       <  2:4382281/93‑1 (MQ=255)
                                                                   tCTTGCCTTTGAACACTTTGCCCAGAGTCCGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTggg                      >  2:1982811/1‑93 (MQ=255)
                                                                    cTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGt                     >  2:1888380/1‑93 (MQ=255)
                                                                     ttGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTa                    <  2:143408/93‑1 (MQ=255)
                                                                       gCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACg                  >  1:3234101/1‑93 (MQ=255)
                                                                       gCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAAACCGGAACGCGGTGAGACAAAGAGATACCGTGAGGCGCGTCCTGGGTACg                  >  2:3508238/1‑93 (MQ=255)
                                                                        ccTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACgg                 >  2:2566186/1‑93 (MQ=255)
                                                                         cTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGa                >  1:2332093/1‑93 (MQ=255)
                                                                          tttGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGag               >  1:504867/1‑92 (MQ=255)
                                                                          tttGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGaa               >  2:1675935/1‑93 (MQ=255)
                                                                          tttGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGaa               <  1:2521436/93‑1 (MQ=255)
                                                                            tGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGt             <  1:3336570/93‑1 (MQ=255)
                                                                             gtaaacTTTGCCCAGAGTCTGGTTCTTACCGCCAGAACCCGGAATGCGGTGAGACAAGGAAGTAACGTGGGTAGCGCTCTGTGTATGGAAGtt            >  2:736637/5‑93 (MQ=255)
                                                                             gAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGAGTCCTGGGCACGGAAgat            >  1:4000739/1‑91 (MQ=255)
                                                                              aaCACTTTGCCCAGAGTCTGGTTCTGCCCCATAGAACCCGGAACGCGGTGAGTCAAGGAGCTACCGTGAGTCGCGTCCTGGGTATGGACGTTc           >  2:4667001/1‑93 (MQ=255)
                                                                              aaCACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTc           <  1:2144361/93‑1 (MQ=255)
                                                                               acacTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGttta          >  2:3731924/1‑91 (MQ=255)
                                                                               acacTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTcc          >  2:2194002/1‑93 (MQ=255)
                                                                               acacTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTcc          >  2:2765796/1‑93 (MQ=255)
                                                                               acacTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTcc          <  2:4242192/93‑1 (MQ=255)
                                                                                cacTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCa         <  2:2198912/93‑1 (MQ=255)
                                                                                 acTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAg        <  2:1153337/93‑1 (MQ=255)
                                                                                   tttGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAgcg      >  1:1111288/1‑93 (MQ=255)
                                                                                   tttGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAgcg      >  1:1000825/1‑93 (MQ=255)
                                                                                    ttGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAgcgc     <  2:383773/93‑1 (MQ=255)
                                                                                     tGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCt    <  1:1357976/93‑1 (MQ=255)
                                                                                       cccAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTa  <  2:3756313/93‑1 (MQ=255)
                                                                                          |                                                                                         
GCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTA  >  NC_000913/3450019‑3450198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTAACGG  >  NC_000913/3450017‑3450202
                                                                                            |                                                                                             
CAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAG                                                                                              >  SRR4302535.1170687/1‑94 (MQ=60)
 AGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGA                                                                                             >  SRR4302535.997609/1‑94 (MQ=60)
 AGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGA                                                                                             >  SRR4302535.2475597/1‑94 (MQ=60)
 AGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGA                                                                                             >  SRR4302535.1170597/1‑94 (MQ=60)
  GCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAG                                                                                            <  SRR4302535.1889686/94‑1 (MQ=60)
   CGTCAACGCGTACTACGTCAAGGCTCTGAACCGTTACCCGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGT                                                                                           <  SRR4302535.3362714/94‑1 (MQ=60)
    GTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTC                                                                                          >  SRR4302535.3570675/1‑94 (MQ=60)
    GTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTC                                                                                          <  SRR4302535.2532871/94‑1 (MQ=60)
     TCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCT                                                                                         >  SRR4302535.584536/1‑94 (MQ=60)
      CAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTG                                                                                        <  SRR4302535.630011/94‑1 (MQ=60)
      CAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTG                                                                                        >  SRR4302535.1822133/1‑94 (MQ=60)
       AACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGG                                                                                       <  SRR4302535.290297/94‑1 (MQ=60)
       AACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGG                                                                                       >  SRR4302535.2811372/1‑94 (MQ=60)
        ACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGT                                                                                      >  SRR4302535.2364286/1‑94 (MQ=60)
           CGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCT                                                                                   >  SRR4302535.4317379/1‑94 (MQ=60)
           CGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCT                                                                                   >  SRR4302535.609288/1‑94 (MQ=60)
            GTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTG                                                                                  >  SRR4302535.5961/1‑94 (MQ=60)
            GTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTG                                                                                  >  SRR4302535.3007934/1‑94 (MQ=60)
               CTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACC                                                                               >  SRR4302535.215257/1‑94 (MQ=60)
                 ACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGA                                                                             >  SRR4302535.245553/1‑94 (MQ=60)
                  CGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGAT                                                                            <  SRR4302535.755755/94‑1 (MQ=60)
                  CGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGAT                                                                            >  SRR4302535.757827/1‑94 (MQ=60)
                    TCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAG                                                                          <  SRR4302535.1059303/94‑1 (MQ=60)
                    TCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAG                                                                          <  SRR4302535.3393341/94‑1 (MQ=60)
                    TCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAG                                                                          >  SRR4302535.3662319/1‑94 (MQ=60)
                    CCAAGGCTCTGATCGGTTACACGTTCGTTCCCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAG                                                                          <  SRR4302535.3730974/94‑1 (MQ=60)
                     CAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGA                                                                         >  SRR4302535.1246081/1‑94 (MQ=60)
                      AAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAA                                                                        >  SRR4302535.4247918/1‑94 (MQ=60)
                      AAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAA                                                                        <  SRR4302535.339100/94‑1 (MQ=60)
                      AAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAA                                                                        >  SRR4302535.932975/1‑94 (MQ=60)
                       AGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAAC                                                                       <  SRR4302535.2690021/94‑1 (MQ=60)
                         GCTCTGAACGGTTACACGTTCGTTCCCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCC                                                                     <  SRR4302535.1843866/94‑1 (MQ=60)
                         GCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCC                                                                     <  SRR4302535.1102315/94‑1 (MQ=60)
                         GCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCC                                                                     >  SRR4302535.3756400/1‑94 (MQ=60)
                          CTCTGAACGGTTACACGATCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAGCCCG                                                                    <  SRR4302535.116961/94‑1 (MQ=60)
                           TCTGACCGGTTCCACGTTCGTTACCCAGCTGACCTGCCATTTTCTTGCCTTTGAACACTATGCCCAGAGTCTGGTTCTGACCGATAGAACCCGG                                                                   <  SRR4302535.1563763/94‑1 (MQ=60)
                             TGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAA                                                                 >  SRR4302535.539974/1‑94 (MQ=60)
                             TGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAA                                                                 <  SRR4302535.3579790/94‑1 (MQ=60)
                              GAACGGTTACACGTTCGTTACCCATCTGCCCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAAC                                                                <  SRR4302535.285697/94‑1 (MQ=60)
                              GAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTGTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAAC                                                                >  SRR4302535.3409054/1‑94 (MQ=60)
                              GAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAAC                                                                <  SRR4302535.448400/94‑1 (MQ=60)
                               AACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACG                                                               >  SRR4302535.240860/1‑94 (MQ=60)
                               AACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACG                                                               >  SRR4302535.2599093/1‑94 (MQ=60)
                               AACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACG                                                               <  SRR4302535.379879/94‑1 (MQ=60)
                               AACGGTTACACGTGCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACG                                                               <  SRR4302535.2150137/94‑1 (MQ=60)
                                ACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGC                                                              <  SRR4302535.857774/94‑1 (MQ=60)
                                 CGGTTACACGTTCGTTACCCATCTGCCCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCG                                                             <  SRR4302535.1465386/94‑1 (MQ=60)
                                  GGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGG                                                            >  SRR4302535.384602/1‑94 (MQ=60)
                                   GTTACACGTTCGTTACCCATCTGACCTACCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGT                                                           <  SRR4302535.177879/94‑1 (MQ=60)
                                     TACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGA                                                         >  SRR4302535.3241913/1‑94 (MQ=60)
                                      ACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAG                                                        <  SRR4302535.459771/94‑1 (MQ=60)
                                       CACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGA                                                       >  SRR4302535.1597386/1‑94 (MQ=60)
                                       CACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCCGACCGATAGAACCCGGAACGCGGTGAGA                                                       >  SRR4302535.4621829/1‑94 (MQ=60)
                                        ACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGAC                                                      >  SRR4302535.1453503/1‑94 (MQ=60)
                                         CGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACA                                                     >  SRR4302535.2910474/1‑94 (MQ=60)
                                             CGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGA                                                 >  SRR4302535.3037597/1‑94 (MQ=60)
                                             CGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGA                                                 <  SRR4302535.114675/94‑1 (MQ=60)
                                             CGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGA                                                 >  SRR4302535.4379364/1‑94 (MQ=60)
                                             CGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGA                                                 >  SRR4302535.49038/1‑94 (MQ=60)
                                              GTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAG                                                <  SRR4302535.3415813/94‑1 (MQ=60)
                                               TTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGT                                               >  SRR4302535.542216/1‑94 (MQ=60)
                                                TACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTA                                              >  SRR4302535.1229662/1‑94 (MQ=60)
                                                 ACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTA                                             >  SRR4302535.1943369/1‑94 (MQ=60)
                                                  CCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTAC                                            >  SRR4302535.2075098/1‑94 (MQ=60)
                                                  CCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTAC                                            >  SRR4302535.1971183/1‑94 (MQ=60)
                                                     ATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGT                                         >  SRR4302535.2514058/1‑94 (MQ=60)
                                                      TCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTG                                        <  SRR4302535.863821/94‑1 (MQ=60)
                                                       CTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGA                                       >  SRR4302535.4539374/1‑94 (MQ=60)
                                                        TGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAG                                      <  SRR4302535.1636054/94‑1 (MQ=60)
                                                         GACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGT                                     <  SRR4302535.1683773/94‑1 (MQ=60)
                                                          ACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTA                                    <  SRR4302535.1953125/94‑1 (MQ=60)
                                                           CCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAG                                   <  SRR4302535.2865369/94‑1 (MQ=60)
                                                           CCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGGAG                                   >  SRR4302535.27517/1‑94 (MQ=60)
                                                             TGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGNNAAGGAGTTACCGTGNGTANCG                                 >  SRR4302535.1871463/1‑94 (MQ=60)
                                                             TGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCG                                 >  SRR4302535.3123428/1‑94 (MQ=60)
                                                             TGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCG                                 >  SRR4302535.3163750/1‑94 (MQ=60)
                                                               CCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTC                               >  SRR4302535.761942/1‑94 (MQ=60)
                                                               CCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTC                               <  SRR4302535.588484/94‑1 (MQ=60)
                                                                 ATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCT                             <  SRR4302535.1031858/94‑1 (MQ=60)
                                                                 ATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCT                             >  SRR4302535.1654570/1‑94 (MQ=60)
                                                                   TTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGG                           >  SRR4302535.3664329/1‑94 (MQ=60)
                                                                   TTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGG                           <  SRR4302535.4398036/94‑1 (MQ=60)
                                                                   TTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGG                           <  SRR4302535.4357888/94‑1 (MQ=60)
                                                                    TTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGG                          <  SRR4302535.2329502/94‑1 (MQ=60)
                                                                    TTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGG                          <  SRR4302535.907472/94‑1 (MQ=60)
                                                                    TTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGG                          <  SRR4302535.3592409/94‑1 (MQ=60)
                                                                    TTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGG                          <  SRR4302535.3813664/94‑1 (MQ=60)
                                                                     TCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGT                         <  SRR4302535.1346169/94‑1 (MQ=60)
                                                                      CTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTA                        >  SRR4302535.3622006/1‑94 (MQ=60)
                                                                      CTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTA                        >  SRR4302535.4064370/1‑94 (MQ=60)
                                                                       TTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTAC                       <  SRR4302535.352260/94‑1 (MQ=60)
                                                                       TTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTAC                       <  SRR4302535.2123416/94‑1 (MQ=60)
                                                                        TGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACG                      >  SRR4302535.3256543/1‑94 (MQ=60)
                                                                         GCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGG                     >  SRR4302535.145449/1‑94 (MQ=60)
                                                                          CCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGA                    >  SRR4302535.2348775/1‑94 (MQ=60)
                                                                           CTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAG                   >  SRR4302535.508396/1‑94 (MQ=60)
                                                                            TTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAG                  <  SRR4302535.3686608/94‑1 (MQ=60)
                                                                            TTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAG                  <  SRR4302535.3301543/94‑1 (MQ=60)
                                                                            TTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAG                  <  SRR4302535.2539604/94‑1 (MQ=60)
                                                                              TGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTT                <  SRR4302535.1949747/94‑1 (MQ=60)
                                                                              TGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTT                <  SRR4302535.1466516/94‑1 (MQ=60)
                                                                              TGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTT                <  SRR4302535.3359806/94‑1 (MQ=60)
                                                                              TGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGAGTCCTGGGCACGGAAGAT                >  SRR4302535.4028109/1‑94 (MQ=60)
                                                                               GAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGCAGCGTCCTGGGTACGGCAGTTC               >  SRR4302535.570202/1‑94 (MQ=60)
                                                                                AACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCC              <  SRR4302535.3240935/94‑1 (MQ=60)
                                                                                AACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCC              <  SRR4302535.2159787/94‑1 (MQ=60)
                                                                                AACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCC              <  SRR4302535.2291873/94‑1 (MQ=60)
                                                                                 ACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCA             <  SRR4302535.546299/94‑1 (MQ=60)
                                                                                 ACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCA             <  SRR4302535.3474680/94‑1 (MQ=60)
                                                                                 ACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCA             <  SRR4302535.2920249/94‑1 (MQ=60)
                                                                                  CGCTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTCCCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAG            <  SRR4302535.4389984/94‑1 (MQ=60)
                                                                                   ACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGC           >  SRR4302535.4698904/1‑94 (MQ=60)
                                                                                    CTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCG          <  SRR4302535.2810739/94‑1 (MQ=60)
                                                                                    CTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCG          >  SRR4302535.1119219/1‑94 (MQ=60)
                                                                                    CTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCG          >  SRR4302535.1007938/1‑94 (MQ=60)
                                                                                    CTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCG          >  SRR4302535.1986745/1‑94 (MQ=60)
                                                                                     TTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGC         >  SRR4302535.2930778/1‑94 (MQ=60)
                                                                                       TGCGCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTT       <  SRR4302535.1600120/94‑1 (MQ=60)
                                                                                       TGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTT       <  SRR4302535.1367688/94‑1 (MQ=60)
                                                                                         CCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTAA     >  SRR4302535.2089743/1‑94 (MQ=60)
                                                                                         CCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTAA     <  SRR4302535.3243884/94‑1 (MQ=60)
                                                                                          CCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTAAC    <  SRR4302535.4050830/94‑1 (MQ=60)
                                                                                          CCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTAAC    <  SRR4302535.2850072/94‑1 (MQ=60)
                                                                                           CAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTAACG   <  SRR4302535.1950329/94‑1 (MQ=60)
                                                                                           CAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTAACG   >  SRR4302535.331510/1‑94 (MQ=60)
                                                                                            AGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTAACGG  >  SRR4302535.1069139/1‑94 (MQ=60)
                                                                                            AGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGGGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTAACGG  >  SRR4302535.3360268/1‑94 (MQ=60)
                                                                                            |                                                                                             
CAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTAACGG  >  NC_000913/3450017‑3450202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑