Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,456,098 A→G Q41Q (CAA→CAG)  yheG → YheG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,456,0980AG99.6% 737.2 / NA 234Q41Q (CAA→CAG) yheGYheG
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (134/98);  total (136/98)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 239 total aligned reads displayed.

GGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCG  >  NC_000913/3456008‑3456186
                                                                                          |                                                                                        
ggCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATCTTCAGCg                                                                                        >  1:502434/1‑93 (MQ=255)
   gcTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCa                                                                                     >  1:3805934/1‑93 (MQ=255)
    cTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAg                                                                                    <  1:216221/93‑1 (MQ=255)
      ggCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGt                                                                                  <  2:1689877/93‑1 (MQ=255)
       gCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGtt                                                                                 <  1:2889620/93‑1 (MQ=255)
       gCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGtt                                                                                 >  1:694873/1‑93 (MQ=255)
         acaACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTgg                                                                               >  1:1137250/1‑93 (MQ=255)
          caACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGc                                                                              >  1:4186644/1‑93 (MQ=255)
           aaCGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGcc                                                                             >  1:2597456/1‑93 (MQ=255)
           aaCGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGcc                                                                             <  2:467472/93‑1 (MQ=255)
             cGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAg                                                                           <  1:3130186/93‑1 (MQ=255)
               aaTTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTg                                                                         >  1:1610234/1‑93 (MQ=255)
                 ttGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGcc                                                                       >  2:4379780/1‑93 (MQ=255)
                   ggTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAg                                                                     <  2:3378162/93‑1 (MQ=255)
                    gTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGa                                                                    >  1:4015211/1‑93 (MQ=255)
                     ttATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGAc                                                                   <  2:3988435/93‑1 (MQ=255)
                      tATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGAcc                                                                  >  2:2282146/1‑93 (MQ=255)
                       aTCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCa                                                                 <  2:2893155/93‑1 (MQ=255)
                       aTCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCa                                                                 <  2:655616/93‑1 (MQ=255)
                        tctcTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCAt                                                                >  2:1457441/1‑93 (MQ=255)
                        tctcTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCAt                                                                <  2:1328582/93‑1 (MQ=255)
                        tctcTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCAt                                                                >  1:1461793/1‑93 (MQ=255)
                        tctcTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCAt                                                                <  2:576975/93‑1 (MQ=255)
                         ctctATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATg                                                               <  2:201934/93‑1 (MQ=255)
                           ctATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGAc                                                             <  1:1179298/93‑1 (MQ=255)
                           ctATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGAc                                                             >  1:1825059/1‑93 (MQ=255)
                            tATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGAcc                                                            >  2:4089630/1‑93 (MQ=255)
                            tATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGAcc                                                            <  1:466769/93‑1 (MQ=255)
                            tATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGCTGGCCAGTGCCAGACCATGAcc                                                            >  2:529155/1‑93 (MQ=255)
                             aTTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCa                                                           >  2:1014484/1‑93 (MQ=255)
                              ttCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAg                                                          <  2:651344/93‑1 (MQ=255)
                              ttCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAg                                                          <  1:3162324/93‑1 (MQ=255)
                                cAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCt                                                        >  1:2980290/1‑93 (MQ=255)
                                 agagGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTAGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCtt                                                       >  2:3912171/1‑93 (MQ=255)
                                  gagGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTg                                                      >  1:3311220/1‑93 (MQ=255)
                                  gagGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTg                                                      >  2:1515644/1‑93 (MQ=255)
                                  gagGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTg                                                      >  1:4426621/1‑93 (MQ=255)
                                    gggCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTc                                                    >  2:1813737/1‑93 (MQ=255)
                                       cGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTcaca                                                 >  1:3242605/1‑93 (MQ=255)
                                        gagaTTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAg                                                <  1:2416781/93‑1 (MQ=255)
                                        gagaTTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAg                                                >  2:2721334/1‑93 (MQ=255)
                                        gagaTTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAg                                                >  2:558783/1‑93 (MQ=255)
                                         agaTTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAgg                                               >  1:908593/1‑93 (MQ=255)
                                          gaTTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGc                                              >  1:611578/1‑93 (MQ=255)
                                          gaTTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGc                                              >  1:2747403/1‑93 (MQ=255)
                                          aattattTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGc                                              <  2:1883428/92‑1 (MQ=255)
                                            ttatttatCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGGCAGACCATGAACAGCTTGTCACAGGCTg                                            >  2:2987034/1‑93 (MQ=255)
                                            ttatttatCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTg                                            >  2:903059/1‑93 (MQ=255)
                                             tatttatCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGa                                           <  2:2648538/93‑1 (MQ=255)
                                             tatttatCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGa                                           >  1:677849/1‑93 (MQ=255)
                                              atttatCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGAt                                          >  1:205361/1‑93 (MQ=255)
                                               tttatCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCCGTGCCAGACCATGACCAGCTTGTCACAGGATGact                                         >  2:3421/1‑91 (MQ=255)
                                               tttatCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGaat                                         >  2:1421384/1‑91 (MQ=255)
                                               tttatCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGAtt                                         >  1:2532714/1‑93 (MQ=255)
                                                 tatCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGAtttt                                       <  1:2857837/93‑1 (MQ=255)
                                                  atCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTg                                      >  2:2528600/1‑93 (MQ=255)
                                                   tctcGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGa                                     >  1:3669484/1‑93 (MQ=255)
                                                   tctcGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGa                                     >  1:4356396/1‑93 (MQ=255)
                                                     tcGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTTACAGGCTGATTTTGGAg                                   >  2:853679/1‑93 (MQ=255)
                                                     tcGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAg                                   >  2:163886/1‑93 (MQ=255)
                                                       gCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCt                                 >  2:4478791/1‑93 (MQ=255)
                                                        ccGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTc                                <  2:686951/93‑1 (MQ=255)
                                                         cGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCg                               <  1:2724337/93‑1 (MQ=255)
                                                         cGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCg                               <  2:2137021/93‑1 (MQ=255)
                                                          gATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTccg                              >  1:1725650/1‑91 (MQ=255)
                                                          gATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCgg                              >  2:310902/1‑93 (MQ=255)
                                                          gATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCgg                              >  1:3909567/1‑93 (MQ=255)
                                                           aTACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGc                             <  2:4286339/93‑1 (MQ=255)
                                                                ccATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGaa                        <  1:2040832/93‑1 (MQ=255)
                                                                ccATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGaa                        <  1:3766374/93‑1 (MQ=255)
                                                                 cATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAg                       <  1:773371/93‑1 (MQ=255)
                                                                 cATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAg                       >  2:1615348/1‑93 (MQ=255)
                                                                  aTTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGc                      <  1:3291102/93‑1 (MQ=255)
                                                                     cGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGt                   <  1:2840967/93‑1 (MQ=255)
                                                                     cGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGt                   <  1:1252619/93‑1 (MQ=255)
                                                                     cGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGt                   <  1:1711736/93‑1 (MQ=255)
                                                                      gAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCggga                  >  1:2220672/1‑91 (MQ=255)
                                                                       aGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTAt                 <  1:3385561/93‑1 (MQ=255)
                                                                        gCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTAtt                <  1:1316063/93‑1 (MQ=255)
                                                                         cGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTc               <  2:2726379/93‑1 (MQ=255)
                                                                          ggTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTcc              <  2:1991867/93‑1 (MQ=255)
                                                                           gTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCa             <  1:4128172/93‑1 (MQ=255)
                                                                           gTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCa             <  2:332880/93‑1 (MQ=255)
                                                                           gTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCa             <  2:235687/93‑1 (MQ=255)
                                                                           gTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCa             >  2:1098824/1‑93 (MQ=255)
                                                                            ttACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCAt            >  2:1255716/1‑93 (MQ=255)
                                                                            ttACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCAt            >  1:1331265/1‑93 (MQ=255)
                                                                            ttACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGGATTCCAt            >  1:4441058/1‑93 (MQ=255)
                                                                             tACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATc           <  1:3535408/93‑1 (MQ=255)
                                                                              aCTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCa          >  2:4142738/1‑93 (MQ=255)
                                                                                tCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGa        >  1:876458/1‑93 (MQ=255)
                                                                                  gATATTCAGCGTCAGGTTGGCCAGTGGCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGGATTCCATCAGAAt      >  1:1538827/1‑93 (MQ=255)
                                                                                  gATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGTAGCGGTATTCCATCAGAAt      <  2:4527544/93‑1 (MQ=255)
                                                                                   atatTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATa     <  2:2473934/93‑1 (MQ=255)
                                                                                    tatTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATAc    >  2:60216/1‑93 (MQ=255)
                                                                                     atTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATAcc   >  2:4171543/1‑93 (MQ=255)
                                                                                     atTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATAcc   >  2:3074992/1‑93 (MQ=255)
                                                                                     atTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATAcc   <  2:2765450/93‑1 (MQ=255)
                                                                                      ttCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCg  >  1:4236675/1‑93 (MQ=255)
                                                                                      ttCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCg  <  2:3708660/93‑1 (MQ=255)
                                                                                          |                                                                                        
GGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCG  >  NC_000913/3456008‑3456186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAG  >  NC_000913/3456005‑3456191
                                                                                             |                                                                                             
TTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAG                                                                                               <  SRR4302535.494583/94‑1 (MQ=60)
  GGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATCTTCAGCG                                                                                             >  SRR4302535.505948/1‑94 (MQ=60)
     CGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCA                                                                                          >  SRR4302535.3832172/1‑94 (MQ=60)
     CGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCA                                                                                          >  SRR4302535.332466/1‑94 (MQ=60)
       CTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGG                                                                                        <  SRR4302535.217705/94‑1 (MQ=60)
        TGGCACAACGAATTGGTTATCTCTATTCAGAGGGCTAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGT                                                                                       <  SRR4302535.4451044/94‑1 (MQ=60)
         GGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTT                                                                                      >  SRR4302535.699748/1‑94 (MQ=60)
          GCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTG                                                                                     <  SRR4302535.2910063/94‑1 (MQ=60)
           tacaccaaatgggtgATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGG                                                                                    <  SRR4302535.1647603/79‑1 (MQ=60)
           CACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGG                                                                                    >  SRR4302535.1145361/1‑94 (MQ=60)
           CACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGG                                                                                    >  SRR4302535.861921/1‑94 (MQ=60)
            ACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGC                                                                                   >  SRR4302535.4215296/1‑94 (MQ=60)
             CAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCC                                                                                  >  SRR4302535.1538174/1‑94 (MQ=60)
             CAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCC                                                                                  >  SRR4302535.2862193/1‑94 (MQ=60)
             CAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCC                                                                                  >  SRR4302535.2616111/1‑94 (MQ=60)
              AACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGGCA                                                                                 >  SRR4302535.3529437/1‑94 (MQ=60)
              AACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCA                                                                                 >  SRR4302535.892159/1‑94 (MQ=60)
              AACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCA                                                                                 >  SRR4302535.1679793/1‑94 (MQ=60)
                CGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGT                                                                               <  SRR4302535.3151987/94‑1 (MQ=60)
                 GAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTG                                                                              >  SRR4302535.3390725/1‑94 (MQ=60)
                 GAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTG                                                                              >  SRR4302535.1621840/1‑94 (MQ=60)
                  AATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGC                                                                             >  SRR4302535.2905273/1‑94 (MQ=60)
                   ATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCC                                                                            >  SRR4302535.3750049/1‑94 (MQ=60)
                   ATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCC                                                                            >  SRR4302535.756928/1‑94 (MQ=60)
                   ATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCC                                                                            >  SRR4302535.948929/1‑94 (MQ=60)
                   ATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCC                                                                            >  SRR4302535.4348811/1‑94 (MQ=60)
                      GGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGA                                                                         >  SRR4302535.4042673/1‑94 (MQ=60)
                          ATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCAT                                                                     >  SRR4302535.1472267/1‑94 (MQ=60)
                           TCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGATACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATG                                                                    >  SRR4302535.3995171/1‑94 (MQ=60)
                             TCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGAC                                                                  >  SRR4302535.1838234/1‑94 (MQ=60)
                              CTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACC                                                                 <  SRR4302535.1187711/94‑1 (MQ=60)
                               TATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCA                                                                <  SRR4302535.470036/94‑1 (MQ=60)
                                ATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCATCGTCAGGTTGGCCAGTGCCAGACCATGACCAC                                                               >  SRR4302535.2867477/1‑94 (MQ=60)
                                 TTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGC                                                              <  SRR4302535.3184257/94‑1 (MQ=60)
                                 TTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGC                                                              <  SRR4302535.260197/94‑1 (MQ=60)
                                 TTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGC                                                              >  SRR4302535.1269977/1‑94 (MQ=60)
                                  TCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCT                                                             >  SRR4302535.3001198/1‑94 (MQ=60)
                                   CAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCTGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTT                                                            >  SRR4302535.4712192/1‑94 (MQ=60)
                                   CAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTT                                                            >  SRR4302535.423735/1‑94 (MQ=60)
                                    AGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTG                                                           >  SRR4302535.4456942/1‑94 (MQ=60)
                                    AGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTG                                                           >  SRR4302535.3334264/1‑94 (MQ=60)
                                         GCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACA                                                      >  SRR4302535.3265128/1‑94 (MQ=60)
                                          CGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAG                                                     >  SRR4302535.2145926/1‑94 (MQ=60)
                                           GAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGG                                                    <  SRR4302535.2434177/94‑1 (MQ=60)
                                           GAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGG                                                    <  SRR4302535.413643/94‑1 (MQ=60)
                                           GAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGG                                                    >  SRR4302535.915044/1‑94 (MQ=60)
                                            AGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGC                                                   >  SRR4302535.2766986/1‑94 (MQ=60)
                                            AGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGC                                                   >  SRR4302535.615836/1‑94 (MQ=60)
                                             GATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCT                                                  <  SRR4302535.3289170/94‑1 (MQ=60)
                                             GATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCT                                                  >  SRR4302535.2453599/1‑94 (MQ=60)
                                              ATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTG                                                 >  SRR4302535.3631518/1‑94 (MQ=60)
                                              ATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTG                                                 >  SRR4302535.3133247/1‑94 (MQ=60)
                                              ATTATTTATCTCGCCGATACGACATTCGAGCGGTTACTCGCTATTCAGCGTCACGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTG                                                 >  SRR4302535.583007/1‑94 (MQ=60)
                                               TTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGA                                                >  SRR4302535.694245/1‑94 (MQ=60)
                                               TTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGA                                                >  SRR4302535.682585/1‑94 (MQ=60)
                                                TATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGAT                                               >  SRR4302535.206768/1‑94 (MQ=60)
                                                 ATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATT                                              >  SRR4302535.2819673/1‑94 (MQ=60)
                                                 ATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATT                                              >  SRR4302535.642879/1‑94 (MQ=60)
                                                 ATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATT                                              >  SRR4302535.2550962/1‑94 (MQ=60)
                                                   TTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTT                                            >  SRR4302535.4330651/1‑94 (MQ=60)
                                                   TTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTT                                            <  SRR4302535.1807860/94‑1 (MQ=60)
                                                    TATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTG                                           <  SRR4302535.1293815/94‑1 (MQ=60)
                                                    TATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTG                                           >  SRR4302535.543676/1‑94 (MQ=60)
                                                    TATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTG                                           <  SRR4302535.2878085/94‑1 (MQ=60)
                                                    TATCTCGCCGATACGCCATTCGAGCGGCTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTG                                           <  SRR4302535.4185764/94‑1 (MQ=60)
                                                     ATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGA                                          >  SRR4302535.3694896/1‑94 (MQ=60)
                                                     ATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGA                                          >  SRR4302535.4386218/1‑94 (MQ=60)
                                                       CTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAG                                        >  SRR4302535.23211/1‑94 (MQ=60)
                                                       CTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAG                                        <  SRR4302535.906214/94‑1 (MQ=60)
                                                        TCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGC                                       <  SRR4302535.1208221/94‑1 (MQ=60)
                                                         CGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCT                                      <  SRR4302535.4461115/94‑1 (MQ=60)
                                                         CGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCT                                      <  SRR4302535.2913912/94‑1 (MQ=60)
                                                          GCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTC                                     >  SRR4302535.3162551/1‑94 (MQ=60)
                                                           CCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCG                                    <  SRR4302535.164769/94‑1 (MQ=60)
                                                           CCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCG                                    >  SRR4302535.1329084/1‑94 (MQ=60)
                                                           CCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCG                                    <  SRR4302535.2760385/94‑1 (MQ=60)
                                                           CCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCG                                    <  SRR4302535.2441270/94‑1 (MQ=60)
                                                            CGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGG                                   <  SRR4302535.2743794/94‑1 (MQ=60)
                                                            CGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGG                                   <  SRR4302535.727820/94‑1 (MQ=60)
                                                            CGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGG                                   >  SRR4302535.3936341/1‑94 (MQ=60)
                                                            CGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGG                                   <  SRR4302535.3908689/94‑1 (MQ=60)
                                                            CGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCCG                                   >  SRR4302535.1738128/1‑94 (MQ=60)
                                                             GATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGC                                  >  SRR4302535.380651/1‑94 (MQ=60)
                                                              ATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCT                                 >  SRR4302535.1157657/1‑94 (MQ=60)
                                                              ATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCT                                 >  SRR4302535.3261601/1‑94 (MQ=60)
                                                                ACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGG                               >  SRR4302535.2218964/1‑94 (MQ=60)
                                                                 CGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGA                              >  SRR4302535.2702535/1‑94 (MQ=60)
                                                                   CCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAG                            <  SRR4302535.3792386/94‑1 (MQ=60)
                                                                   CCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAG                            <  SRR4302535.3877350/94‑1 (MQ=60)
                                                                   CCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAG                            <  SRR4302535.2055531/94‑1 (MQ=60)
                                                                   CCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAG                            <  SRR4302535.2055532/94‑1 (MQ=60)
                                                                    CATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGC                           <  SRR4302535.778812/94‑1 (MQ=60)
                                                                    CATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGC                           >  SRR4302535.3251074/1‑94 (MQ=60)
                                                                     ATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCG                          >  SRR4302535.4680453/1‑94 (MQ=60)
                                                                     ATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCG                          >  SRR4302535.551385/1‑94 (MQ=60)
                                                                     ATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCG                          <  SRR4302535.3313985/94‑1 (MQ=60)
                                                                        CGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTA                       <  SRR4302535.1261576/94‑1 (MQ=60)
                                                                        CGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTA                       <  SRR4302535.3012524/94‑1 (MQ=60)
                                                                        CGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTA                       <  SRR4302535.2861119/94‑1 (MQ=60)
                                                                        CGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTA                       <  SRR4302535.1724115/94‑1 (MQ=60)
                                                                        CGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGGA                       >  SRR4302535.2236479/1‑94 (MQ=60)
                                                                         GAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTAT                      <  SRR4302535.2632690/94‑1 (MQ=60)
                                                                          AGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATT                     <  SRR4302535.3409120/94‑1 (MQ=60)
                                                                           GCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTC                    <  SRR4302535.1325463/94‑1 (MQ=60)
                                                                            CGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCC                   <  SRR4302535.1881369/94‑1 (MQ=60)
                                                                            CGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCC                   <  SRR4302535.1889252/94‑1 (MQ=60)
                                                                              GTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCAT                 <  SRR4302535.4156422/94‑1 (MQ=60)
                                                                              GTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCAT                 >  SRR4302535.1340763/1‑94 (MQ=60)
                                                                              GTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCAT                 <  SRR4302535.4047856/94‑1 (MQ=60)
                                                                              GTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCAT                 <  SRR4302535.3386712/94‑1 (MQ=60)
                                                                              GTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCAT                 >  SRR4302535.461912/1‑94 (MQ=60)
                                                                              GTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGGATTCCAT                 >  SRR4302535.4471506/1‑94 (MQ=60)
                                                                                TACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATTACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCA               <  SRR4302535.173224/94‑1 (MQ=60)
                                                                                TACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCA               <  SRR4302535.3559951/94‑1 (MQ=60)
                                                                                  CTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGA             >  SRR4302535.882665/1‑94 (MQ=60)
                                                                                    CGATATTCAGCGTCAGGTTGGCCAGTGGCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGGATTCCATCAGAAT           >  SRR4302535.1549919/1‑94 (MQ=60)
                                                                                    CGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAAT           <  SRR4302535.137753/94‑1 (MQ=60)
                                                                                    CGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAAT           <  SRR4302535.2126594/94‑1 (MQ=60)
                                                                                       TATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTTCATCAGAATACC        >  SRR4302535.1590821/1‑94 (MQ=60)
                                                                                        ATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCG       >  SRR4302535.1473972/1‑94 (MQ=60)
                                                                                        ATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCG       >  SRR4302535.4265669/1‑94 (MQ=60)
                                                                                         TTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGG      <  SRR4302535.3666486/94‑1 (MQ=60)
                                                                                           CAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTG    <  SRR4302535.2027772/94‑1 (MQ=60)
                                                                                            AGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGA   <  SRR4302535.1556693/94‑1 (MQ=60)
                                                                                            AGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGA   >  SRR4302535.2256674/1‑94 (MQ=60)
                                                                                            AGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGA   >  SRR4302535.2021911/1‑94 (MQ=60)
                                                                                            AGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGA   >  SRR4302535.3828235/1‑94 (MQ=60)
                                                                                             GCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAG  <  SRR4302535.955711/94‑1 (MQ=60)
                                                                                             GCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAG  <  SRR4302535.3626132/94‑1 (MQ=60)
                                                                                             |                                                                                             
TTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAG  >  NC_000913/3456005‑3456191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑