Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,484,054 T→C Y100H (TAC→CAC)  crp → cyclic AMP receptor protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,484,0540TC100.0% 642.8 / NA 202Y100H (TAC→CAC) crpcyclic AMP receptor protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (92/110);  total (92/110)

Only 100 of 205 total aligned reads displayed.

TTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCA  >  NC_000913/3483964‑3484143
                                                                                          |                                                                                         
tttATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCAc                                                                                         <  1:1096966/93‑1 (MQ=255)
   aTTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACaaa                                                                                      <  1:2162945/93‑1 (MQ=255)
    ttGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACaaaa                                                                                     >  1:1483575/1‑93 (MQ=255)
     tGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACaaaaa                                                                                    <  2:1477143/93‑1 (MQ=255)
      ggCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACaaaaaa                                                                                   >  2:4257086/1‑93 (MQ=255)
       gCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAAt                                                                                  <  2:311270/93‑1 (MQ=255)
        cGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAAtt                                                                                 <  1:4121078/93‑1 (MQ=255)
        cGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAAtt                                                                                 <  1:779732/93‑1 (MQ=255)
         gAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAAttt                                                                                <  1:3254656/93‑1 (MQ=255)
         gAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAAttt                                                                                >  2:1635788/1‑93 (MQ=255)
          aaCTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTc                                                                               <  1:2839108/93‑1 (MQ=255)
           aCTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCg                                                                              >  1:2559671/1‑93 (MQ=255)
             tGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGcc                                                                            >  2:4645482/1‑93 (MQ=255)
               ggCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCaa                                                                          <  1:451500/93‑1 (MQ=255)
                gCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAAt                                                                         <  1:3974346/93‑1 (MQ=255)
                gCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAAt                                                                         <  1:341169/93‑1 (MQ=255)
                 ccTGTTTGAAGAGGGCCAGTAACGTAGCGCAGGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAAtt                                                                        <  2:4656681/93‑1 (MQ=255)
                 ccTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAAtt                                                                        <  2:3214839/93‑1 (MQ=255)
                 ccTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAAtt                                                                        <  1:221734/93‑1 (MQ=255)
                 ccTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAAtt                                                                        >  1:2360858/1‑93 (MQ=255)
                 ccTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAAtt                                                                        <  1:3933958/93‑1 (MQ=255)
                   tGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGa                                                                      <  1:565758/93‑1 (MQ=255)
                    gTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGGCAATTGAt                                                                     >  2:878005/1‑93 (MQ=255)
                    gTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGAt                                                                     >  1:954084/1‑93 (MQ=255)
                    gTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGAt                                                                     <  1:3880409/93‑1 (MQ=255)
                    gTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGAt                                                                     >  2:1609079/1‑93 (MQ=255)
                       tGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCa                                                                  <  2:871456/93‑1 (MQ=255)
                       tGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCa                                                                  <  1:3779609/93‑1 (MQ=255)
                         aaGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAgg                                                                <  2:516230/93‑1 (MQ=255)
                             gggCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTaaa                                                            <  2:4053272/93‑1 (MQ=255)
                              ggCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAAc                                                           <  1:1177185/93‑1 (MQ=255)
                              ggCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAAc                                                           <  1:4012867/93‑1 (MQ=255)
                               gCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAAcc                                                          <  1:226532/93‑1 (MQ=255)
                               gCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAAcc                                                          <  1:214449/93‑1 (MQ=255)
                               gCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAAcc                                                          >  2:2805285/1‑93 (MQ=255)
                                 cAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCg                                                        >  2:3770084/1‑93 (MQ=255)
                                  aGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCgg                                                       >  1:856076/1‑93 (MQ=255)
                                  aGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCgg                                                       >  2:512941/1‑93 (MQ=255)
                                   ggAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGa                                                      >  1:1047886/1‑93 (MQ=255)
                                    gAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGAc                                                     >  1:2656147/1‑93 (MQ=255)
                                     aaCGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACa                                                    <  2:3587115/93‑1 (MQ=255)
                                      aCGTAGCGCATGGGTACGTGCCAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACAt                                                   <  2:26315/93‑1 (MQ=255)
                                        gTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTc                                                 <  2:2757299/93‑1 (MQ=255)
                                        gTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTc                                                 <  2:3259617/93‑1 (MQ=255)
                                        gTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTc                                                 <  1:322009/93‑1 (MQ=255)
                                         tAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCt                                                <  2:1783291/93‑1 (MQ=255)
                                         tAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCt                                                <  1:1911186/93‑1 (MQ=255)
                                          aGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTg                                               >  2:3823642/1‑93 (MQ=255)
                                            cgcATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGAt                                             <  2:3017286/93‑1 (MQ=255)
                                             gcATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATg                                            >  1:1148924/1‑93 (MQ=255)
                                             gcATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATg                                            <  1:4074538/93‑1 (MQ=255)
                                                tGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGt                                         >  2:2670490/1‑93 (MQ=255)
                                                tGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGt                                         <  2:2877166/93‑1 (MQ=255)
                                                 gggTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGtt                                        <  2:2724833/93‑1 (MQ=255)
                                                  ggTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGttt                                       <  2:1279891/93‑1 (MQ=255)
                                                  ggTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGttt                                       <  2:2158413/93‑1 (MQ=255)
                                                  ggTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGttt                                       <  2:2054260/93‑1 (MQ=255)
                                                   gTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTg                                      >  1:4664334/1‑93 (MQ=255)
                                                     aCGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTc                                    <  1:3113885/93‑1 (MQ=255)
                                                     aCGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTc                                    <  2:4117740/93‑1 (MQ=255)
                                                     aCGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTc                                    <  2:4338735/93‑1 (MQ=255)
                                                      cGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCt                                   <  1:3003645/93‑1 (MQ=255)
                                                          cGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGcac                               <  2:416602/93‑1 (MQ=255)
                                                           gAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGcaca                              >  1:1614727/1‑93 (MQ=255)
                                                            aaaaCCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAg                             >  1:4170917/1‑93 (MQ=255)
                                                             aaaCCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGa                            <  1:2816292/93‑1 (MQ=255)
                                                             aaaCCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGa                            <  2:300644/93‑1 (MQ=255)
                                                             aaaCCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGa                            <  2:3551144/93‑1 (MQ=255)
                                                             aaaCCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGa                            <  2:4457365/93‑1 (MQ=255)
                                                              aaCCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGAt                           >  1:3681686/1‑93 (MQ=255)
                                                              aaCCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGAt                           <  2:3340953/93‑1 (MQ=255)
                                                                 cGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGgc                        >  1:2699447/1‑93 (MQ=255)
                                                                 cGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGgc                        >  2:1969164/1‑93 (MQ=255)
                                                                 cGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCCGATGCGTTTGTCTGCACAGATGgc                        <  1:3369935/93‑1 (MQ=255)
                                                                   ccTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGgcgc                      <  2:3171509/93‑1 (MQ=255)
                                                                    cTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGcgcg                     >  1:1121276/1‑93 (MQ=255)
                                                                    cTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGcgcg                     >  1:2925675/1‑93 (MQ=255)
                                                                    cTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGcgcg                     >  1:247252/1‑93 (MQ=255)
                                                                     tgtgAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGcgt                    >  2:2430016/1‑93 (MQ=255)
                                                                     tgtgAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGcgt                    >  2:3674490/1‑93 (MQ=255)
                                                                      gtgAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGcgtc                   >  1:1304901/1‑93 (MQ=255)
                                                                       tgAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGcgtcg                  >  1:142917/1‑93 (MQ=255)
                                                                        gAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGcgtcgt                 >  2:2096961/1‑93 (MQ=255)
                                                                          aGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCt               <  1:4266288/93‑1 (MQ=255)
                                                                           gTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTg              >  2:442899/1‑93 (MQ=255)
                                                                            tGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGc             <  2:1035271/93‑1 (MQ=255)
                                                                            tGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGc             <  2:616660/93‑1 (MQ=255)
                                                                            tGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGc             >  2:3458101/1‑93 (MQ=255)
                                                                              gCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCaa           <  2:2311133/93‑1 (MQ=255)
                                                                              gCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCaa           >  1:4300237/1‑93 (MQ=255)
                                                                                tGAAATTTCGCACAAAACATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGt         <  1:1719285/93‑1 (MQ=255)
                                                                                tGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGt         <  1:536094/93‑1 (MQ=255)
                                                                                tGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGt         >  1:4374493/1‑93 (MQ=255)
                                                                                tGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGt         >  2:3680060/1‑93 (MQ=255)
                                                                                 gAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTc        >  1:1691227/1‑93 (MQ=255)
                                                                                 gAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTc        >  2:1520807/1‑93 (MQ=255)
                                                                                    aTTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAATTTACt     >  2:3087639/1‑93 (MQ=255)
                                                                                    aTTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACt     >  1:192377/1‑93 (MQ=255)
                                                                                    aTTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACt     <  1:3737068/93‑1 (MQ=255)
                                                                                       tCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCa  >  1:4596891/1‑93 (MQ=255)
                                                                                          |                                                                                         
TTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCA  >  NC_000913/3483964‑3484143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGA  >  NC_000913/3483962‑3484147
                                                                                            |                                                                                             
ATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCA                                                                                              >  SRR4302535.2275922/1‑94 (MQ=60)
ATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCA                                                                                              >  SRR4302535.689114/1‑94 (MQ=60)
ATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCA                                                                                              <  SRR4302535.468803/94‑1 (MQ=60)
 TTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCAC                                                                                             <  SRR4302535.218779/94‑1 (MQ=60)
 TTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCAC                                                                                             <  SRR4302535.1207663/94‑1 (MQ=60)
 TTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCAC                                                                                             <  SRR4302535.3143103/94‑1 (MQ=60)
  TTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACA                                                                                            <  SRR4302535.1104777/94‑1 (MQ=60)
   TTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAA                                                                                           <  SRR4302535.1633674/94‑1 (MQ=60)
     ATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAA                                                                                         <  SRR4302535.2178463/94‑1 (MQ=60)
     ATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAA                                                                                         >  SRR4302535.1494187/1‑94 (MQ=60)
        GGCGAACTGGACCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAAT                                                                                      <  SRR4302535.1139733/94‑1 (MQ=60)
          CGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTT                                                                                    <  SRR4302535.785206/94‑1 (MQ=60)
          CGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTT                                                                                    <  SRR4302535.4149280/94‑1 (MQ=60)
           GAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTC                                                                                   <  SRR4302535.3277268/94‑1 (MQ=60)
           GAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTC                                                                                   <  SRR4302535.3923353/94‑1 (MQ=60)
            AACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCG                                                                                  <  SRR4302535.2859248/94‑1 (MQ=60)
            AACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCG                                                                                  >  SRR4302535.2578099/1‑94 (MQ=60)
              CTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCC                                                                                >  SRR4302535.460073/1‑94 (MQ=60)
                GGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAA                                                                              <  SRR4302535.4172921/94‑1 (MQ=60)
                 GGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAAT                                                                             <  SRR4302535.454662/94‑1 (MQ=60)
                  GCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATT                                                                            >  SRR4302535.2377804/1‑94 (MQ=60)
                  GCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATT                                                                            <  SRR4302535.1072167/94‑1 (MQ=60)
                  GCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATT                                                                            <  SRR4302535.343535/94‑1 (MQ=60)
                  GCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATT                                                                            <  SRR4302535.4001493/94‑1 (MQ=60)
                   CCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTG                                                                           <  SRR4302535.223261/94‑1 (MQ=60)
                   CCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTG                                                                           <  SRR4302535.3960804/94‑1 (MQ=60)
                   CCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTG                                                                           >  SRR4302535.3141756/1‑94 (MQ=60)
                   CCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTG                                                                           >  SRR4302535.4063111/1‑94 (MQ=60)
                   CCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTG                                                                           <  SRR4302535.1106603/94‑1 (MQ=60)
                    CTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGA                                                                          >  SRR4302535.3377678/1‑94 (MQ=60)
                     TGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGAT                                                                         <  SRR4302535.569720/94‑1 (MQ=60)
                     TGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGAT                                                                         >  SRR4302535.960844/1‑94 (MQ=60)
                      GTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATT                                                                        <  SRR4302535.3907044/94‑1 (MQ=60)
                      GTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATT                                                                        <  SRR4302535.1711282/94‑1 (MQ=60)
                         TGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAG                                                                     <  SRR4302535.3805695/94‑1 (MQ=60)
                            AGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTA                                                                  >  SRR4302535.1003598/1‑94 (MQ=60)
                               GGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAAC                                                               <  SRR4302535.2645979/94‑1 (MQ=60)
                                GGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACC                                                              <  SRR4302535.4040318/94‑1 (MQ=60)
                                GGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACC                                                              >  SRR4302535.1570260/1‑94 (MQ=60)
                                GGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACC                                                              <  SRR4302535.1185587/94‑1 (MQ=60)
                                 GCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCC                                                             <  SRR4302535.215919/94‑1 (MQ=60)
                                 GCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCC                                                             <  SRR4302535.228089/94‑1 (MQ=60)
                                   CAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGG                                                           <  SRR4302535.2171401/94‑1 (MQ=60)
                                   CAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGG                                                           >  SRR4302535.2676683/1‑94 (MQ=60)
                                   CAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGG                                                           >  SRR4302535.862110/1‑94 (MQ=60)
                                   CAGGAACGTAGCGCATGCGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCTCCAATTGATTCAGGTAAACCCGG                                                           <  SRR4302535.2908223/94‑1 (MQ=60)
                                    AGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGA                                                          >  SRR4302535.1055316/1‑94 (MQ=60)
                                    AGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGA                                                          >  SRR4302535.1176726/1‑94 (MQ=60)
                                     GGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGAC                                                         >  SRR4302535.2675177/1‑94 (MQ=60)
                                      GAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACA                                                        <  SRR4302535.3733187/94‑1 (MQ=60)
                                          GTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCT                                                    <  SRR4302535.324239/94‑1 (MQ=60)
                                          GTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCT                                                    >  SRR4302535.2281749/1‑94 (MQ=60)
                                           TAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTG                                                   <  SRR4302535.4315443/94‑1 (MQ=60)
                                           TAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTG                                                   <  SRR4302535.1924959/94‑1 (MQ=60)
                                           TAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTG                                                   <  SRR4302535.325464/94‑1 (MQ=60)
                                              CGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATG                                                >  SRR4302535.1157107/1‑94 (MQ=60)
                                              CGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATG                                                >  SRR4302535.710401/1‑94 (MQ=60)
                                              CGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATG                                                >  SRR4302535.468101/1‑94 (MQ=60)
                                              CGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATG                                                <  SRR4302535.3053055/94‑1 (MQ=60)
                                               GCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGC                                               <  SRR4302535.4102415/94‑1 (MQ=60)
                                                CATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCG                                              <  SRR4302535.4004327/94‑1 (MQ=60)
                                                  TGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTT                                            <  SRR4302535.3994821/94‑1 (MQ=60)
                                                    GGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTG                                          >  SRR4302535.4695870/1‑94 (MQ=60)
                                                       ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCT                                       <  SRR4302535.2271210/94‑1 (MQ=60)
                                                       ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCT                                       <  SRR4302535.3135561/94‑1 (MQ=60)
                                                        CGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTG                                      <  SRR4302535.3024699/94‑1 (MQ=60)
                                                        CGTGCGAAAACCCCCTGTAAAGTGTCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTG                                      <  SRR4302535.1429636/94‑1 (MQ=60)
                                                          TGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCA                                    <  SRR4302535.4184395/94‑1 (MQ=60)
                                                            CGAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACA                                  >  SRR4302535.1626366/1‑94 (MQ=60)
                                                             GAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAG                                 <  SRR4302535.794152/94‑1 (MQ=60)
                                                             GAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAG                                 >  SRR4302535.4199475/1‑94 (MQ=60)
                                                             GAAAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAG                                 <  SRR4302535.2081285/94‑1 (MQ=60)
                                                               AAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGAT                               >  SRR4302535.3707166/1‑94 (MQ=60)
                                                               AAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGAT                               <  SRR4302535.2836297/94‑1 (MQ=60)
                                                               AAACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGAT                               <  SRR4302535.1884971/94‑1 (MQ=60)
                                                                AACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATG                              <  SRR4302535.3429288/94‑1 (MQ=60)
                                                                 ACCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGG                             <  SRR4302535.2599175/94‑1 (MQ=60)
                                                                  CCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGC                            >  SRR4302535.2718738/1‑94 (MQ=60)
                                                                  CCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGC                            <  SRR4302535.55767/94‑1 (MQ=60)
                                                                  CCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGC                            <  SRR4302535.2501997/94‑1 (MQ=60)
                                                                  CCGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGC                            <  SRR4302535.4466449/94‑1 (MQ=60)
                                                                   CGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCG                           <  SRR4302535.2953895/94‑1 (MQ=60)
                                                                   CGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCG                           <  SRR4302535.513774/94‑1 (MQ=60)
                                                                   CGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCG                           >  SRR4302535.4287665/1‑94 (MQ=60)
                                                                   CGCCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCCGATGCGTTTGTCTGCACAGATGGCG                           <  SRR4302535.3393380/94‑1 (MQ=60)
                                                                     CCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCG                         >  SRR4302535.1129272/1‑94 (MQ=60)
                                                                     CCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCG                         >  SRR4302535.2946300/1‑94 (MQ=60)
                                                                     CCTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCG                         >  SRR4302535.248946/1‑94 (MQ=60)
                                                                      CTGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGT                        >  SRR4302535.4389466/1‑94 (MQ=60)
                                                                       TGTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTC                       >  SRR4302535.1314217/1‑94 (MQ=60)
                                                                        GTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCG                      >  SRR4302535.143913/1‑94 (MQ=60)
                                                                        GTGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCG                      <  SRR4302535.2769038/94‑1 (MQ=60)
                                                                         TGAAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGT                     >  SRR4302535.4103718/1‑94 (MQ=60)
                                                                           AAGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGACTGCACAGATGGCGCGTCGTCT                   >  SRR4302535.3810769/1‑94 (MQ=60)
                                                                            AGTGGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTG                  <  SRR4302535.4295500/94‑1 (MQ=60)
                                                                               TGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAA               <  SRR4302535.3590907/94‑1 (MQ=60)
                                                                               GGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAA               >  SRR4302535.4329666/1‑94 (MQ=60)
                                                                               GGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAA               >  SRR4302535.1295703/1‑94 (MQ=60)
                                                                               GGCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAA               >  SRR4302535.2192915/1‑94 (MQ=60)
                                                                                GCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAG              <  SRR4302535.889708/94‑1 (MQ=60)
                                                                                GCTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAG              <  SRR4302535.1934086/94‑1 (MQ=60)
                                                                                 CTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGT             >  SRR4302535.4404423/1‑94 (MQ=60)
                                                                                 CTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGT             <  SRR4302535.1484860/94‑1 (MQ=60)
                                                                                 CTGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTGTGTCTGCACAGATGGCGCGTCGTCTGCAAGT             >  SRR4302535.378574/1‑94 (MQ=60)
                                                                                  TGAAATTTCGCACAAAACATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTC            <  SRR4302535.1731719/94‑1 (MQ=60)
                                                                                  TGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTC            <  SRR4302535.539839/94‑1 (MQ=60)
                                                                                  TGAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTC            >  SRR4302535.1703465/1‑94 (MQ=60)
                                                                                   GAAATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCA           <  SRR4302535.2482697/94‑1 (MQ=60)
                                                                                     AATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACT         >  SRR4302535.193704/1‑94 (MQ=60)
                                                                                      ATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTT        <  SRR4302535.3762932/94‑1 (MQ=60)
                                                                                      ATTTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTT        >  SRR4302535.284778/1‑94 (MQ=60)
                                                                                        TTCGCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCA      >  SRR4302535.4628164/1‑94 (MQ=60)
                                                                                           GCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAG   <  SRR4302535.4293609/94‑1 (MQ=60)
                                                                                           GCACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAG   <  SRR4302535.4577460/94‑1 (MQ=60)
                                                                                            CACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGA  <  SRR4302535.1337990/94‑1 (MQ=60)
                                                                                            CACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGA  >  SRR4302535.1744975/1‑94 (MQ=60)
                                                                                            CACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGA  >  SRR4302535.2053839/1‑94 (MQ=60)
                                                                                            |                                                                                             
ATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGA  >  NC_000913/3483962‑3484147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑