Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,554,088	+C	coding (2/624 nt)	yhgKJ ←	RNA phosphage cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,554,088	1	.	C	99.4%	511.6 / NA	167	S133W (TCG→TGG)	yhgKJ	RNA phosphage cyclase
Reads supporting (aligned to +/- strand): ref base . (0/0); major base C (75/91); minor base A (0/1); total (75/92)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 191 total aligned reads displayed.

GGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCG‑AGGCGGCGCCGACGGGTTATCGGTCGCCG‑CGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTT  >  NC_000913/3553997‑3554174

ggCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCgc                                                                                         >  2:4292903/1‑92 (MQ=255)

ggCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCgc                                                                                         <  1:2037585/93‑2 (MQ=255)

 gCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCgca                                                                                        <  2:3408015/93‑3 (MQ=255)

 gCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCgca                                                                                        <  2:3263730/93‑3 (MQ=255)

   aGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCgcagg                                                                                      <  2:2769838/93‑5 (MQ=255)

     ggTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAggcg                                                                                    <  1:467013/93‑1 (MQ=255)

     ggTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAggcg                                                                                    <  1:3768107/93‑1 (MQ=255)

     ggTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAggcg                                                                                    <  1:1677783/93‑1 (MQ=255)

       tAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAggcggc                                                                                  >  1:44947/1‑93 (MQ=255)

       tAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAggcggc                                                                                  <  1:2455368/93‑1 (MQ=255)

        aaaaaCCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGgcggcg                                                                                 <  2:528995/93‑1 (MQ=255)

         aaaaCCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGgcgc                                                                                >  2:3649304/1‑93 (MQ=255)

           aaCCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCg                                                                              <  2:2095728/93‑1 (MQ=255)

            aCCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGgcggcggcgg                                                                             >  2:1707025/1‑92 (MQ=255)

            aCCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGa                                                                             <  1:2917223/93‑1 (MQ=255)

            aCCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGa                                                                             <  1:3929968/93‑1 (MQ=255)

             ccGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAc                                                                            >  2:4065401/1‑93 (MQ=255)

              cGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACg                                                                           >  1:1314167/1‑93 (MQ=255)

               gTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACgg                                                                          <  1:2577166/93‑1 (MQ=255)

               gTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACgg                                                                          <  2:3908899/93‑1 (MQ=255)

                tGGCGTAACAGCGTGGTTTGCTGATGAATTTCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGCCggg                                                                         >  2:1950450/1‑93 (MQ=255)

                    gTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTAt                                                                     <  2:4350456/93‑1 (MQ=255)

                    gTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGGTAt                                                                     >  2:2469655/1‑93 (MQ=255)

                    gTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGCTAt                                                                     >  1:4414753/1‑93 (MQ=255)

                     tAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGGGGCGCCGACGGGTTATc                                                                    >  2:4675165/1‑93 (MQ=255)

                     tAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATc                                                                    <  2:3977082/93‑1 (MQ=255)

                     tAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATc                                                                    <  2:639150/93‑1 (MQ=255)

                       aCAGCGTGGTTTGCTTATGAATTCCTATTTGCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCgg                                                                  <  1:3407853/93‑1 (MQ=255)

                       aCAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCgg                                                                  >  2:2727153/1‑93 (MQ=255)

                       aCAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCgg                                                                  >  2:1057179/1‑93 (MQ=255)

                        cAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGTCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGt                                                                 <  2:2043058/93‑1 (MQ=255)

                        cAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGt                                                                 <  1:1720934/93‑1 (MQ=255)

                        cAGCGTGGTTTGCTGATGAATGCCTATGTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGt                                                                 <  1:2094044/93‑1 (MQ=255)

                         aGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGtg                                                                >  2:3155794/1‑92 (MQ=255)

                         aGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGtg                                                                <  1:4205965/93‑2 (MQ=255)

                          gCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGtgc                                                               <  1:452125/93‑3 (MQ=255)

                          gCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATACGCAGTCGGCGCCGACGGGGTATTgggga                                                               >  2:3411375/1‑90 (MQ=255)

                              ggTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGt‑gccg‑cc                                                         <  2:1994066/93‑2 (MQ=255)

                              ggTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGt‑gccg‑cc                                                         >  2:3004909/1‑92 (MQ=255)

                              ggTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGt‑gccg‑cc                                                         >  1:693952/1‑92 (MQ=255)

                               gTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCCGCGGATAAAATCCGCAGGCGGCGCCGACGGGGTATCGGt‑gccg‑ccg                                                        >  1:4096567/1‑91 (MQ=255)

                                 ttGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGt‑gccg‑ccgct                                                      <  1:3119883/93‑5 (MQ=255)

                                    cTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCAc                                                    <  2:3195842/93‑1 (MQ=255)

                                    cTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCAc                                                    <  2:3693440/93‑1 (MQ=255)

                                     tgatgaATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACt                                                   >  2:3313969/1‑93 (MQ=255)

                                     tgatgaATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACt                                                   >  2:2867230/1‑93 (MQ=255)

                                      gatgaATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACtt                                                  >  1:1256687/1‑93 (MQ=255)

                                      gatgaATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGGTATCGGT‑GCCGCCGCTCACtt                                                  >  1:4193217/1‑93 (MQ=255)

                                      gatgaATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGAGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACtt                                                  >  2:4344219/1‑93 (MQ=255)

                                      gatgaATCCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACtt                                                  <  1:890229/93‑1 (MQ=255)

                                         gaATTCCTATTTTCGCCAGCAGCGGCTCTAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCaa                                               >  1:1507279/1‑93 (MQ=255)

                                         gaATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCCCCGCCGGGTTATCGGT‑GCCGCCGCTCACTTCaa                                               >  1:663363/1‑93 (MQ=255)

                                          aaTTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAac                                              >  2:3833969/1‑93 (MQ=255)

                                          aaTTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGG‑GGCCGCCGCTCCCTTCAac                                              >  1:822970/1‑93 (MQ=255)

                                           aTTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTTCCGC‑CGCTCACTTCAaca                                             >  1:3831162/1‑93 (MQ=255)

                                           aTTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAaca                                             <  2:1890220/93‑1 (MQ=255)

                                                tATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGa                                        <  1:2958011/93‑1 (MQ=255)

                                                 aTTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGaa                                       >  1:1889856/1‑93 (MQ=255)

                                                  ttttCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAg                                      >  2:2285909/1‑93 (MQ=255)

                                                   tttCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAgg                                     >  2:722825/1‑93 (MQ=255)

                                                    ttCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGt                                    <  2:3377508/93‑1 (MQ=255)

                                                    tgcGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGt                                    <  1:4031449/91‑1 (MQ=255)

                                                      caccAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTcc                                  <  2:4598739/91‑1 (MQ=255)

                                                       gCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCa                                 <  1:3438597/93‑1 (MQ=255)

                                                        gcagcagCGGCGCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCAt                                <  1:786299/92‑1 (MQ=255)

                                                        ccAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCAt                                <  2:4326968/93‑1 (MQ=255)

                                                        ccAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCAt                                <  1:2457941/93‑1 (MQ=255)

                                                        ccAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCAt                                <  2:2732523/93‑1 (MQ=255)

                                                         cagcagCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATc                               <  2:2953682/93‑1 (MQ=255)

                                                         cagcagCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATc                               <  1:3583968/93‑1 (MQ=255)

                                                         cagcagCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATc                               <  1:2271638/93‑1 (MQ=255)

                                                          agcagcGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCg                              <  1:3723252/93‑1 (MQ=255)

                                                           gcagcGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCgg                             >  2:2525845/1‑93 (MQ=255)

                                                             agcGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCa                           <  2:1774752/93‑1 (MQ=255)

                                                              gcGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCaa                          >  2:482215/1‑93 (MQ=255)

                                                              gcGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGGTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCaa                          >  2:4418267/1‑93 (MQ=255)

                                                                  cTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCACACca                      >  1:1112556/1‑93 (MQ=255)

                                                                  cTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACca                      >  2:167501/1‑93 (MQ=255)

                                                                  cTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACca                      <  1:570019/93‑1 (MQ=255)

                                                                     cAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAg                   <  1:1473037/93‑1 (MQ=255)

                                                                     cAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAg                   >  1:2880699/1‑93 (MQ=255)

                                                                     cAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAg                   <  1:4648121/93‑1 (MQ=255)

                                                                       gCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAgcg                 <  1:3302346/93‑1 (MQ=255)

                                                                           ccGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCggg             <  1:707844/93‑1 (MQ=255)

                                                                            cggcggATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGgc            >  2:3299450/1‑93 (MQ=255)

                                                                             ggcggATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGgca           <  2:1802458/93‑1 (MQ=255)

                                                                              gcggATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGgcag          >  2:821224/1‑93 (MQ=255)

                                                                               cggATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGgcagc         >  2:1498242/1‑93 (MQ=255)

                                                                                ggATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTCCCGC‑CGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGgcagca        >  1:2954037/1‑93 (MQ=255)

                                                                                ggATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGgcagca        >  2:3993928/1‑93 (MQ=255)

                                                                                ggATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGgcagca        >  1:3623930/1‑93 (MQ=255)

                                                                                ggATAAAACCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGgcagca        <  2:3555395/93‑1 (MQ=255)

                                                                                 gATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCAc       >  2:308630/1‑93 (MQ=255)

                                                                                  aTAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCAcc      <  1:3334387/93‑1 (MQ=255)

                                                                                   tAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCg     >  1:3682815/1‑93 (MQ=255)

                                                                                   tAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCg     <  2:2241888/93‑1 (MQ=255)

                                                                                     aaaTCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGGAAACCACAGCGCGGGCCGCACCGtt   >  2:249021/1‑93 (MQ=255)

                                                                                     aaaTCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGtt   >  1:99594/1‑93 (MQ=255)

                                                                                     aaaTCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGtt   <  1:3082436/93‑1 (MQ=255)

                                                                                      aaTCCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGttt  >  2:4371608/1‑93 (MQ=255)

GGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCG‑AGGCGGCGCCGACGGGTTATCGGTCGCCG‑CGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTT  >  NC_000913/3553997‑3554174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCGGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCG‑AGGCGGCGCCGACGGGTTATCGGT‑‑CGCCG‑CGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCAC  >  NC_000913/3553995‑3554181

CCGGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCG                                                                                                   <  SRR4302535.4426273/94‑1 (MQ=60)

CCGGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCG                                                                                                   <  SRR4302535.2629543/94‑1 (MQ=60)

  GGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGca                                                                                                 <  SRR4302535.2052259/94‑3 (MQ=60)

    CAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGG                                                                                               <  SRR4302535.1286705/94‑1 (MQ=60)

     AGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGcaggg                                                                                              >  SRR4302535.1390800/1‑89 (MQ=60)

     AGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGC                                                                                              >  SRR4302535.3883440/1‑94 (MQ=60)

     AGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGC                                                                                              <  SRR4302535.3731548/94‑1 (MQ=60)

      GGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCG                                                                                             <  SRR4302535.4223547/94‑1 (MQ=60)

       GGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGG                                                                                            <  SRR4302535.470282/94‑1 (MQ=60)

       GGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGG                                                                                            <  SRR4302535.470283/94‑1 (MQ=60)

       GGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGG                                                                                            <  SRR4302535.1689933/94‑1 (MQ=60)

       GGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGG                                                                                            <  SRR4302535.3794121/94‑1 (MQ=60)

        GTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGC                                                                                           >  SRR4302535.45286/1‑94 (MQ=60)

        GTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGC                                                                                           <  SRR4302535.2007159/94‑1 (MQ=60)

         TAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCG                                                                                          <  SRR4302535.2473071/94‑1 (MQ=60)

           AAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCC                                                                                        >  SRR4302535.730828/1‑94 (MQ=60)

            AAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCG                                                                                       <  SRR4302535.3135521/94‑1 (MQ=60)

             AACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGA                                                                                      >  SRR4302535.896950/1‑94 (MQ=60)

              ACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC                                                                                     <  SRR4302535.2937816/94‑1 (MQ=60)

              ACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC                                                                                     <  SRR4302535.3956807/94‑1 (MQ=60)

               CCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACG                                                                                    <  SRR4302535.1681434/94‑1 (MQ=60)

               CCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACG                                                                                    >  SRR4302535.1323560/1‑94 (MQ=60)

                 GTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGG                                                                                  <  SRR4302535.2959765/94‑1 (MQ=60)

                 GTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGG                                                                                  <  SRR4302535.2595687/94‑1 (MQ=60)

                     CGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGCTAT                                                                              >  SRR4302535.4444968/1‑94 (MQ=60)

                        AACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGG                                                                           <  SRR4302535.3640785/94‑1 (MQ=60)

                        AACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGG                                                                           <  SRR4302535.2920072/94‑1 (MQ=60)

                         ACAGCGTGGTTTGCTTATGAATTCCTATTTGCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT                                                                          <  SRR4302535.3431575/94‑1 (MQ=60)

                          CAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑G                                                                       <  SRR4302535.1733380/94‑1 (MQ=60)

                          CAGCGTGGTTTGCTGATGAATGCCTATGTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑G                                                                       <  SRR4302535.2109141/94‑1 (MQ=60)

                           AGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTgc                                                                        >  SRR4302535.1862513/1‑92 (MQ=60)

                           AGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTgc                                                                        <  SRR4302535.4234747/94‑3 (MQ=60)

                            GCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCC                                                                    <  SRR4302535.455291/94‑1 (MQ=60)

                            GCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCC                                                                    <  SRR4302535.4530302/94‑1 (MQ=60)

                               TGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTGCCGCC                                                                    >  SRR4302535.698820/1‑94 (MQ=60)

                                GGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCCGCGGATAAAATCCGCAGGCGGCGCCGACGGGGTATCGGTGCCGCCG                                                                   >  SRR4302535.4124605/1‑94 (MQ=60)

                                 GTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCACGCGGAGCCGACGGGGTATCGGTGGCGCCG‑C                                                                 >  SRR4302535.2799551/1‑94 (MQ=60)

                                  TTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTgccgccgct                                                                 <  SRR4302535.1501200/94‑10 (MQ=60)

                                  TTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGAAGGCGGCGCCGACGGGTTATCGGTgccgccgct                                                                 <  SRR4302535.4280444/94‑10 (MQ=60)

                                   TTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTgccgccgctc                                                                <  SRR4302535.3141605/94‑11 (MQ=60)

                                      CTGATGAATTCCTTTTTCCGCCAGCAGCGGCTCCAGCGCCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACT                                                          <  SRR4302535.4686438/94‑1 (MQ=60)

                                      CTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACT                                                          >  SRR4302535.1699575/1‑94 (MQ=60)

                                      CTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACT                                                          <  SRR4302535.222715/94‑1 (MQ=60)

                                       TGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTT                                                         >  SRR4302535.602997/1‑94 (MQ=60)

                                       TGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTT                                                         >  SRR4302535.1265676/1‑94 (MQ=60)

                                       TGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGGTATCGGT‑‑‑GCCGCCGCTCACTT                                                         >  SRR4302535.4221911/1‑94 (MQ=60)

                                        GATGAATCCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTC                                                        <  SRR4302535.896537/94‑1 (MQ=60)

                                         ATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCA                                                       <  SRR4302535.3865538/94‑1 (MQ=60)

                                          TGAATTCCTATTTTCGCCAGCAGCGGCTCTAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAA                                                      >  SRR4302535.1518115/1‑94 (MQ=60)

                                          TGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCCCCGCCGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAA                                                      >  SRR4302535.667998/1‑94 (MQ=60)

                                           GAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGggccgccgctcccttcaac                                                        >  SRR4302535.828739/1‑75 (MQ=60)

                                           GAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAAC                                                     <  SRR4302535.883003/94‑1 (MQ=60)

                                            AATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTTCCGC‑‑‑CGCTCACTTCAACA                                                    >  SRR4302535.3857581/1‑94 (MQ=60)

                                            AATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACA                                                    <  SRR4302535.2052591/94‑1 (MQ=60)

                                             ATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCCGCGGATAAAATCCGCAGGCGGCGCCGACGGGGTATCGG‑‑‑CGCCGCCGCTCACTTCAACAC                                                   >  SRR4302535.3098410/1‑94 (MQ=60)

                                                CCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCG                                                >  SRR4302535.2977012/1‑94 (MQ=60)

                                                CCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGGTATCGGT‑‑‑GCCGCCGCTGACTTCAACACGCG                                                >  SRR4302535.3817587/1‑94 (MQ=60)

                                                  TATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAA                                              >  SRR4302535.1903467/1‑94 (MQ=60)

                                                  TATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAA                                              <  SRR4302535.2978785/94‑1 (MQ=60)

                                                     TTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGT                                           <  SRR4302535.2779712/94‑1 (MQ=60)

                                                     TTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGT                                           <  SRR4302535.3376783/94‑1 (MQ=60)

                                                      TGCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTC                                          <  SRR4302535.4059028/94‑1 (MQ=60)

                                                         GCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCAT                                       <  SRR4302535.3462515/94‑1 (MQ=60)

                                                          GCAGCAGCGGCGCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATC                                      <  SRR4302535.791809/94‑1 (MQ=60)

                                                          CCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATC                                      <  SRR4302535.3750937/94‑1 (MQ=60)

                                                          CCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATC                                      <  SRR4302535.2475668/94‑1 (MQ=60)

                                                           CAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCG                                     <  SRR4302535.3608825/94‑1 (MQ=60)

                                                           CAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCG                                     <  SRR4302535.2287839/94‑1 (MQ=60)

                                                            AGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGG                                    <  SRR4302535.3749043/94‑1 (MQ=60)

                                                            AGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGG                                    >  SRR4302535.3680157/1‑94 (MQ=60)

                                                                  GGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACC                              <  SRR4302535.3154698/94‑1 (MQ=60)

                                                                   GCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCACACCA                             >  SRR4302535.1120493/1‑94 (MQ=60)

                                                                   GCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCA                             <  SRR4302535.3964793/94‑1 (MQ=60)

                                                                    CTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCAC                            <  SRR4302535.574021/94‑1 (MQ=60)

                                                                     TCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACA                           <  SRR4302535.700583/94‑1 (MQ=60)

                                                                     TCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAACGTCCATCGGCAAACCACA                           >  SRR4302535.2393369/1‑94 (MQ=60)

                                                                      CCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAG                          >  SRR4302535.2901090/1‑94 (MQ=60)

                                                                       CAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC                         <  SRR4302535.674279/94‑1 (MQ=60)

                                                                       CAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC                         <  SRR4302535.1483583/94‑1 (MQ=60)

                                                                       CAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC                         <  SRR4302535.4679605/94‑1 (MQ=60)

                                                                         GCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGC                       <  SRR4302535.3325311/94‑1 (MQ=60)

                                                                          CACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCG                      <  SRR4302535.254423/94‑1 (MQ=60)

                                                                           CCCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGG                     <  SRR4302535.1328310/94‑1 (MQ=60)

                                                                           ACGCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGG                     <  SRR4302535.4195451/94‑1 (MQ=60)

                                                                            CCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGG                    >  SRR4302535.4571109/1‑94 (MQ=60)

                                                                            CCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGG                    >  SRR4302535.3478554/1‑94 (MQ=60)

                                                                             CCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGC                   <  SRR4302535.712811/94‑1 (MQ=60)

                                                                                 ccgacAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCA               <  SRR4302535.4455539/89‑1 (MQ=60)

                                                                                 CGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑CCCGC‑CGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCA               >  SRR4302535.2974788/1‑94 (MQ=60)

                                                                                 CGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCA               >  SRR4302535.3649049/1‑94 (MQ=60)

                                                                                    ATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCG            <  SRR4302535.3357611/94‑1 (MQ=60)

                                                                                    ATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCG            >  SRR4302535.3708301/1‑94 (MQ=60)

                                                                                     TAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGT           <  SRR4302535.4076366/94‑1 (MQ=60)

                                                                                      AAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAANCCACAGCGCGGGCAGCACCNTT          >  SRR4302535.3334908/1‑94 (MQ=60)

                                                                                      AAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTT          >  SRR4302535.100294/1‑94 (MQ=60)

                                                                                       AAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTT         <  SRR4302535.3103918/94‑1 (MQ=60)

                                                                                        AATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTG        >  SRR4302535.4161319/1‑94 (MQ=60)

                                                                                         ATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGC       >  SRR4302535.4488454/1‑94 (MQ=60)

                                                                                             C‑AGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCAC  <  SRR4302535.2922473/94‑1 (MQ=60)

CCGGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCG‑AGGCGGCGCCGACGGGTTATCGGT‑‑CGCCG‑CGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCAC  >  NC_000913/3553995‑3554181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`