Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,554,088 +C coding (2/624 nt) yhgKJ ← RNA phosphage cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,554,0881.C99.3% 841.9 / ‑1.8 276coding (2/624 nt)yhgKJRNA phosphage cyclase
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base C (119/153);  total (120/156)
Fisher's exact test for biased strand distribution p-value = 5.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 323 total aligned reads displayed.

CGGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCG‑AGGCGGCGCCGACGGGTTATCGGTCGCCG‑CGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTT  >  NC_000913/3553996‑3554174
                                                                                               |                                                                                       
cGGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCg                                                                                          >  1:2529814/1‑93 (MQ=255)
  gCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCgca                                                                                        <  2:2007471/93‑3 (MQ=255)
  gCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCgca                                                                                        >  1:1571286/1‑91 (MQ=255)
  gCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCgca                                                                                        <  1:3131097/93‑3 (MQ=255)
  gCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCgca                                                                                        <  2:5199264/93‑3 (MQ=255)
   cAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCgcag                                                                                       <  1:3481472/93‑4 (MQ=255)
   cAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCgcag                                                                                       <  2:3791241/93‑4 (MQ=255)
   cAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCgcag                                                                                       >  1:996005/1‑90 (MQ=255)
   cAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCgcag                                                                                       <  1:7639815/93‑4 (MQ=255)
    aGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCgcagg                                                                                      <  1:4637534/93‑5 (MQ=255)
    aGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCgcagg                                                                                      >  1:2067689/1‑89 (MQ=255)
    aGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCgcagg                                                                                      >  1:7867292/1‑89 (MQ=255)
    aGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCgcagg                                                                                      >  1:629414/1‑89 (MQ=255)
      ggTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAggcg                                                                                    >  2:6616310/1‑93 (MQ=255)
       gTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAggcgg                                                                                   >  1:818687/1‑93 (MQ=255)
        tAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAggcggg                                                                                  >  2:4006947/1‑92 (MQ=255)
          aaaaCCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGgcgc                                                                                <  1:4847479/93‑1 (MQ=255)
            aaCCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGCTAAAAT‑CCGCAGGCGGCGCCg                                                                              >  2:5891119/1‑93 (MQ=255)
             aCCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGa                                                                             <  1:3322931/93‑1 (MQ=255)
              ccGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGAc                                                                            >  1:4063635/1‑93 (MQ=255)
              ccGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGAc                                                                            >  2:4364937/1‑93 (MQ=255)
                gTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACgg                                                                          >  1:5875338/1‑93 (MQ=255)
                gTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACgg                                                                          <  2:7108558/93‑1 (MQ=255)
                gTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACgg                                                                          >  1:1862388/1‑93 (MQ=255)
                 tGGCGTANCAGCGTGGTTTGCNGNTGNATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACggg                                                                         <  1:3640573/93‑1 (MQ=255)
                 tGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGCTAAAAT‑CCGTAGGCGGCGCCGACggg                                                                         >  1:2626179/1‑93 (MQ=255)
                 tGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACggg                                                                         <  1:2279115/93‑1 (MQ=255)
                    cGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTa                                                                      <  2:4912845/93‑1 (MQ=255)
                      tAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATc                                                                    <  1:2985458/93‑1 (MQ=255)
                        aCAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCgg                                                                  >  2:3105270/1‑93 (MQ=255)
                        aCAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCgg                                                                  <  2:2382767/93‑1 (MQ=255)
                        aCAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGAGGATAAAAT‑CCGCAGGCGGCGCCGATGGGTTATCgg                                                                  >  2:4183465/1‑93 (MQ=255)
                         cAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCggg                                                                 >  2:2828084/1‑92 (MQ=255)
                         cAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGt                                                                 <  1:3920346/93‑1 (MQ=255)
                          aGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGtg                                                                <  2:6125962/93‑2 (MQ=255)
                           gCGTGGTTTGCTGATGAATTCCTATTTTCCCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGtgc                                                               <  2:3418857/93‑3 (MQ=255)
                            cGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGtgcc                                                              <  2:5952734/93‑4 (MQ=255)
                            cGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGtgcc                                                              <  1:342853/93‑4 (MQ=255)
                             gTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGtgccg                                                             >  1:6893634/1‑89 (MQ=255)
                              tGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGGTATCGGt‑gccg‑c                                                          >  2:3652426/1‑93 (MQ=255)
                               ggTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGt‑gccg‑cc                                                         >  1:5820635/1‑92 (MQ=255)
                                 tttGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGt‑gccg‑ccgc                                                       <  1:5705079/93‑4 (MQ=255)
                                  ttGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACCGGGTATCGGt‑gccg‑ccgct                                                      >  1:3053362/1‑89 (MQ=255)
                                      tgatgaATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACt                                                   >  2:3329543/1‑93 (MQ=255)
                                      tgatgaATTCCTATTTTCGCCAGCAGCGGCTCCAC‑CACCCGGCGGCTAAAAT‑GCGCAGGCAGCGCCCACGGGTTATCGGT‑GCCGCCGCTCACt                                                   >  2:1355230/1‑93 (MQ=255)
                                       gatgaATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACtt                                                  >  1:6977695/1‑93 (MQ=255)
                                       gatgaATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACtt                                                  >  2:545271/1‑93 (MQ=255)
                                       gatgaATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACtt                                                  >  2:530499/1‑93 (MQ=255)
                                       gatgaATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACtc                                                  >  1:1440177/1‑92 (MQ=255)
                                       gatgaATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGG‑GGCCGCCGCTCACtt                                                  >  2:6837683/1‑93 (MQ=255)
                                       gatgaATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGAGCCGACGGGTTATCGGT‑GCCGCCGCTCACtt                                                  >  2:1853502/1‑93 (MQ=255)
                                        atgaATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTc                                                 <  2:6971299/93‑1 (MQ=255)
                                        atgaATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGGTATCGGT‑GCCGCCGCTCACTTc                                                 >  2:6730897/1‑93 (MQ=255)
                                           aaTTCCTATGTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAac                                              <  1:1914806/93‑1 (MQ=255)
                                            aTTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAaca                                             <  2:756923/93‑1 (MQ=255)
                                              tCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACAcg                                           >  1:2789640/1‑93 (MQ=255)
                                               ccTATTTTCGCCAGCAGCGGCTCCAGTCACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACAcg                                           <  2:6347659/93‑1 (MQ=255)
                                                cTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACAcgcg                                         >  1:4324470/1‑93 (MQ=255)
                                                 tATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGa                                        <  2:4628966/93‑1 (MQ=255)
                                                   ttttCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAg                                      >  2:562508/1‑93 (MQ=255)
                                                   ttttCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAg                                      <  2:7653509/93‑1 (MQ=255)
                                                   ttttCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAg                                      >  1:2178055/1‑93 (MQ=255)
                                                   ttttCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAg                                      >  1:2501137/1‑93 (MQ=255)
                                                   ttttCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAg                                      >  2:7380892/1‑93 (MQ=255)
                                                   ttttCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGGTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAg                                      >  1:1773624/1‑93 (MQ=255)
                                                     ttCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGt                                    <  2:1164765/93‑1 (MQ=255)
                                                     ttCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGt                                    <  1:641154/93‑1 (MQ=255)
                                                      tCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTc                                   <  1:4915411/93‑1 (MQ=255)
                                                       cGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTcc                                  <  2:1261524/93‑1 (MQ=255)
                                                        gCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCa                                 <  1:3766266/93‑1 (MQ=255)
                                                        gCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCa                                 <  2:6170089/93‑1 (MQ=255)
                                                         ccAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTTAACACGCGAAGGTCCAt                                >  2:479386/1‑93 (MQ=255)
                                                         ccAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCAt                                >  1:5988828/1‑93 (MQ=255)
                                                         ccAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCAt                                <  1:4455036/93‑1 (MQ=255)
                                                          cagcagCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATc                               <  2:2270082/93‑1 (MQ=255)
                                                          cagcagCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATc                               <  1:5178464/93‑1 (MQ=255)
                                                          cagcagCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATc                               <  1:7464618/93‑1 (MQ=255)
                                                          cagcagCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATc                               >  2:1423817/1‑93 (MQ=255)
                                                          cagcagCGCCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATc                               <  2:3028899/93‑1 (MQ=255)
                                                           agcagcGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCg                              <  2:6892954/93‑1 (MQ=255)
                                                           agcagcGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCg                              <  2:2724092/93‑1 (MQ=255)
                                                            gcagcGGCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCgg                             >  2:1954379/1‑93 (MQ=255)
                                                                  gCTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAAcc                       >  1:1280686/1‑93 (MQ=255)
                                                                   cTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACca                      <  2:494389/93‑1 (MQ=255)
                                                                   cTCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACca                      <  2:392357/93‑1 (MQ=255)
                                                                    tCCAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACcac                     >  2:1642190/1‑93 (MQ=255)
                                                                      cAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAg                   <  2:778142/93‑1 (MQ=255)
                                                                      cAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAg                   <  1:1967168/93‑1 (MQ=255)
                                                                      cAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAg                   <  1:6736135/93‑1 (MQ=255)
                                                                      cAG‑CACCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAg                   <  2:4246956/93‑1 (MQ=255)
                                                                       aG‑CACCCGGCGGATAAAAT‑CCNCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAgc                  <  1:1063808/93‑1 (MQ=255)
                                                                          cgcccGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAgcgc                <  2:2216569/91‑1 (MQ=255)
                                                                           aCCCGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGcgcg               >  1:6159226/1‑93 (MQ=255)
                                                                            cccGGCGGATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCgg              <  1:5243141/93‑1 (MQ=255)
                                                                            cccGACGGATAAAATACAC‑AGGCGGCGCCGACGGGTTATCGGAACCGC‑CGCTCACTTCAACACGCGAAGGTCCATCAGCAAACCACAGcacga              <  2:7037722/93‑2 (MQ=255)
                                                                              cggcggATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGgc            <  2:6249280/93‑1 (MQ=255)
                                                                               ggcggATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGgca           >  2:3577745/1‑93 (MQ=255)
                                                                               ggcggATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGgca           >  1:4214801/1‑93 (MQ=255)
                                                                                   gATAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCAc       >  1:7816421/1‑93 (MQ=255)
                                                                                    aTAAAAT‑CCGCAGGCGGCGCCGACGGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCAcc      >  2:5109746/1‑93 (MQ=255)
                                                                                               |                                                                                       
CGGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAG‑CACCCGGCGGATAAAAT‑CCG‑AGGCGGCGCCGACGGGTTATCGGTCGCCG‑CGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTT  >  NC_000913/3553996‑3554174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCGGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCG‑AGGCGGCGCCGACGGGTTATCGGT‑‑CGCCG‑CGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCA  >  NC_000913/3553995‑3554177
                                                                                             |                                                                                             
CCGGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCG                                                                                               >  SRR4302543.2552832/1‑94 (MQ=60)
CCGGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCG                                                                                               <  SRR4302543.7197003/94‑1 (MQ=60)
CCGGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCG                                                                                               <  SRR4302543.1474345/94‑1 (MQ=60)
  GGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGca                                                                                             >  SRR4302543.1585311/1‑92 (MQ=60)
   GCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGcag                                                                                            >  SRR4302543.1004805/1‑91 (MQ=60)
   GCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGcag                                                                                            <  SRR4302543.3159259/94‑4 (MQ=60)
   GCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGcag                                                                                            <  SRR4302543.4108716/94‑4 (MQ=60)
   GCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGcag                                                                                            <  SRR4302543.2051744/94‑4 (MQ=60)
   GCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGcag                                                                                            <  SRR4302543.1955734/94‑4 (MQ=60)
   GCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGcag                                                                                            <  SRR4302543.6790615/94‑4 (MQ=60)
   GCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGcag                                                                                            <  SRR4302543.6419689/94‑4 (MQ=60)
    CAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGG                                                                                           >  SRR4302543.635166/1‑94 (MQ=60)
    CAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGG                                                                                           <  SRR4302543.3512653/94‑1 (MQ=60)
    CAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGG                                                                                           <  SRR4302543.7712287/94‑1 (MQ=60)
    CAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGG                                                                                           >  SRR4302543.7941371/1‑94 (MQ=60)
    CAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGG                                                                                           >  SRR4302543.2086175/1‑94 (MQ=60)
    CAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCAAGCACCCGGCGGATAAAATCCGCAGG                                                                                           >  SRR4302543.1478465/1‑94 (MQ=60)
     AGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGcaggg                                                                                          >  SRR4302543.5907248/1‑89 (MQ=60)
     AGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGC                                                                                          <  SRR4302543.3366656/94‑1 (MQ=60)
     AGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGC                                                                                          <  SRR4302543.4679708/94‑1 (MQ=60)
      GGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCG                                                                                         <  SRR4302543.4375891/94‑1 (MQ=60)
      GGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCG                                                                                         <  SRR4302543.99625/94‑1 (MQ=60)
      GGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCG                                                                                         <  SRR4302543.7257009/94‑1 (MQ=60)
      GGGTAAAAACCGTGGCGTAACAGCGTGGGTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCG                                                                                         >  SRR4302543.4985568/1‑94 (MQ=60)
       GGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGG                                                                                        >  SRR4302543.825893/1‑94 (MQ=60)
         GAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCG                                                                                      <  SRR4302543.4834503/94‑1 (MQ=60)
           AAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCC                                                                                    <  SRR4302543.4891684/94‑1 (MQ=60)
             AACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGC                                                                                  >  SRR4302543.2791780/1‑94 (MQ=60)
             AACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGA                                                                                  <  SRR4302543.7720464/94‑1 (MQ=60)
             AACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGA                                                                                  <  SRR4302543.1340515/94‑1 (MQ=60)
              ACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC                                                                                 <  SRR4302543.3352907/94‑1 (MQ=60)
              ACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC                                                                                 >  SRR4302543.4100021/1‑94 (MQ=60)
              ACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC                                                                                 <  SRR4302543.3717284/94‑1 (MQ=60)
               CCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACG                                                                                <  SRR4302543.525590/94‑1 (MQ=60)
                CGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGG                                                                               >  SRR4302543.5928861/1‑94 (MQ=60)
                CGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGG                                                                               >  SRR4302543.1879119/1‑94 (MQ=60)
                CGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCAGACGG                                                                               >  SRR4302543.5897534/1‑94 (MQ=60)
                 GTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGCTAAAATCCGTAGGCGGCGCCGACGGG                                                                              >  SRR4302543.2649948/1‑94 (MQ=60)
                  TGGCGTANCAGCGTGGTTTGCNGNTGNATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGT                                                                             <  SRR4302543.3672607/94‑1 (MQ=60)
                  TGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGT                                                                             <  SRR4302543.2299956/94‑1 (MQ=60)
                  TGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGT                                                                             <  SRR4302543.948404/94‑1 (MQ=60)
                   GGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTT                                                                            <  SRR4302543.6255033/94‑1 (MQ=60)
                      GTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATC                                                                         >  SRR4302543.301580/1‑94 (MQ=60)
                      GTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATC                                                                         >  SRR4302543.2792518/1‑94 (MQ=60)
                      GTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGGTATC                                                                         >  SRR4302543.2391870/1‑94 (MQ=60)
                       gaacatggtggttttgTGATGAATTCCTATTGTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG                                                                        <  SRR4302543.2181532/78‑1 (MQ=60)
                       TAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG                                                                        <  SRR4302543.3012571/94‑1 (MQ=60)
                       TAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG                                                                        <  SRR4302543.3012570/94‑1 (MQ=60)
                       TAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG                                                                        <  SRR4302543.5404809/94‑1 (MQ=60)
                       TAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCG                                                                        <  SRR4302543.5941604/94‑1 (MQ=60)
                        AACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGG                                                                       <  SRR4302543.3477657/94‑1 (MQ=60)
                        AACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGG                                                                       <  SRR4302543.6029594/94‑1 (MQ=60)
                         ACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT                                                                      <  SRR4302543.7671014/94‑1 (MQ=60)
                         ACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT                                                                      <  SRR4302543.3094/94‑1 (MQ=60)
                         ACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT                                                                      <  SRR4302543.31827/94‑1 (MQ=60)
                          CAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑G                                                                   <  SRR4302543.3954943/94‑1 (MQ=60)
                            GCGTGGTTTGGTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCC                                                                <  SRR4302543.1862700/94‑1 (MQ=60)
                            GCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCC                                                                <  SRR4302543.4848772/94‑1 (MQ=60)
                            GCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCC                                                                <  SRR4302543.3808534/94‑1 (MQ=60)
                             CGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGGGGGGCCGACGGGTTATCGctggcg                                                                  >  SRR4302543.1841009/1‑88 (MQ=60)
                             CGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCG                                                               >  SRR4302543.6958436/1‑94 (MQ=60)
                             CGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCG                                                               <  SRR4302543.346013/94‑1 (MQ=60)
                              GTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCG‑C                                                             >  SRR4302543.1929760/1‑94 (MQ=60)
                              GTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCG‑C                                                             >  SRR4302543.6356458/1‑94 (MQ=60)
                               TGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTGCCGCC                                                                <  SRR4302543.4553641/94‑1 (MQ=60)
                               TGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTGCCGCC                                                                >  SRR4302543.5873564/1‑94 (MQ=60)
                               TGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTGCCGCC                                                                <  SRR4302543.7874645/94‑1 (MQ=60)
                               TGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTGCCGCC                                                                <  SRR4302543.3103189/94‑1 (MQ=60)
                                 GTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGCAGGGTTATCGGTGCCGCCG‑C                                                             >  SRR4302543.2211827/1‑94 (MQ=60)
                                 GTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTGCCGCCG‑C                                                             >  SRR4302543.6476100/1‑94 (MQ=60)
                                 GTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGCTATCGGTGCCGCCG‑C                                                             >  SRR4302543.2211826/1‑94 (MQ=60)
                                  TTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTgccgccgct                                                             >  SRR4302543.1566294/1‑85 (MQ=60)
                                  TTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTgccgccgct                                                             <  SRR4302543.5757117/94‑10 (MQ=60)
                                  TTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACCGGGTATCGGTgccgccgct                                                             >  SRR4302543.3080862/1‑85 (MQ=60)
                                   TTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTgccgccgctc                                                            >  SRR4302543.2324513/1‑84 (MQ=60)
                                   TTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTgccgccgctc                                                            >  SRR4302543.2298230/1‑84 (MQ=60)
                                    TGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGTGTTATCGGT‑‑‑GCCGCCGCTCA                                                        <  SRR4302543.7737628/94‑1 (MQ=60)
                                    TGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTgccgccgctcc                                                           >  SRR4302543.3101351/1‑83 (MQ=60)
                                    TGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCA                                                        <  SRR4302543.6691047/94‑1 (MQ=60)
                                     GCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCAC                                                       <  SRR4302543.3462791/94‑1 (MQ=60)
                                      CTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACT                                                      >  SRR4302543.1322468/1‑94 (MQ=60)
                                       TGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTgccgccgctcactc                                                        >  SRR4302543.1453190/1‑80 (MQ=60)
                                       TGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTT                                                     <  SRR4302543.6322591/94‑1 (MQ=60)
                                       TGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTT                                                     >  SRR4302543.7043359/1‑94 (MQ=60)
                                            AATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACA                                                >  SRR4302543.1203431/1‑94 (MQ=60)
                                            AATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACA                                                >  SRR4302543.2621150/1‑94 (MQ=60)
                                            AATTCCTATGTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACA                                                <  SRR4302543.1932066/94‑1 (MQ=60)
                                            AATGCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACA                                                <  SRR4302543.3179669/94‑1 (MQ=60)
                                             ATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAATTCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACAC                                               <  SRR4302543.1968318/94‑1 (MQ=60)
                                             ATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACAC                                               <  SRR4302543.7020415/94‑1 (MQ=60)
                                             ATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACAC                                               >  SRR4302543.6119219/1‑94 (MQ=60)
                                             ATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACAC                                               <  SRR4302543.207809/94‑1 (MQ=60)
                                              TTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACG                                              >  SRR4302543.2815106/1‑94 (MQ=60)
                                                CCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCG                                            >  SRR4302543.4363733/1‑94 (MQ=60)
                                                CCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCG                                            >  SRR4302543.4593933/1‑94 (MQ=60)
                                                 CTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGA                                           >  SRR4302543.2826942/1‑94 (MQ=60)
                                                   ATTTTCGCCAGCAGCGGCTCCAGCACGCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAG                                         <  SRR4302543.4492233/94‑1 (MQ=60)
                                                   ATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAG                                         <  SRR4302543.4492234/94‑1 (MQ=60)
                                                   ATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAG                                         >  SRR4302543.2523836/1‑94 (MQ=60)
                                                   ATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAG                                         >  SRR4302543.2197576/1‑94 (MQ=60)
                                                   ATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGGTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAG                                         >  SRR4302543.1789474/1‑94 (MQ=60)
                                                    TTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGG                                        <  SRR4302543.4198979/94‑1 (MQ=60)
                                                    TTTTCCCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGG                                        <  SRR4302543.4241634/94‑1 (MQ=60)
                                                     TTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATGAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGT                                       <  SRR4302543.5926121/94‑1 (MQ=60)
                                                     TTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGT                                       <  SRR4302543.4319067/94‑1 (MQ=60)
                                                      TTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTC                                      <  SRR4302543.647005/94‑1 (MQ=60)
                                                      TTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTC                                      <  SRR4302543.1717309/94‑1 (MQ=60)
                                                       TCTCCAGCAGCGGCTCCAGTATCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCC                                     <  SRR4302543.7906176/94‑1 (MQ=60)
                                                       TCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCC                                     <  SRR4302543.2123502/94‑1 (MQ=60)
                                                       TCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCC                                     <  SRR4302543.4960199/94‑1 (MQ=60)
                                                       TCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCC                                     <  SRR4302543.3747568/94‑1 (MQ=60)
                                                        CGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCA                                    <  SRR4302543.2192587/94‑1 (MQ=60)
                                                         GCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCAT                                   >  SRR4302543.6043712/1‑94 (MQ=60)
                                                         GCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCAT                                   >  SRR4302543.7629055/1‑94 (MQ=60)
                                                         GCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCAT                                   <  SRR4302543.3799420/94‑1 (MQ=60)
                                                          CCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATC                                  <  SRR4302543.3692631/94‑1 (MQ=60)
                                                          CCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATC                                  <  SRR4302543.4495388/94‑1 (MQ=60)
                                                          CCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATC                                  <  SRR4302543.1800541/94‑1 (MQ=60)
                                                          CCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATC                                  >  SRR4302543.5188482/1‑94 (MQ=60)
                                                           CAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCG                                 <  SRR4302543.5225210/94‑1 (MQ=60)
                                                           CAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCG                                 <  SRR4302543.7536126/94‑1 (MQ=60)
                                                            AGCAGCTGCTCCAGCACGCGGCGGATAAAAGCCGCAGGCGGCGCCGACGGGTTATCGGTGCGGCCT‑CG‑‑CACTTCAACACGCGAAGGTCCATCGG                                <  SRR4302543.3293366/94‑1 (MQ=40)
                                                            AGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGG                                <  SRR4302543.4483115/94‑1 (MQ=60)
                                                             GCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGC                               <  SRR4302543.2419161/94‑1 (MQ=60)
                                                             GCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGC                               >  SRR4302543.7042812/1‑94 (MQ=60)
                                                              CAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACTGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAAGACCATCGGCA                              >  SRR4302543.5090130/1‑94 (MQ=60)
                                                               AGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAA                             <  SRR4302543.610761/94‑1 (MQ=60)
                                                                GCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAA                            >  SRR4302543.8005209/1‑94 (MQ=60)
                                                                 CGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAAC                           <  SRR4302543.5261151/94‑1 (MQ=60)
                                                                  GGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACC                          >  SRR4302543.1292103/1‑94 (MQ=60)
                                                                   GCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCA                         <  SRR4302543.3642828/94‑1 (MQ=60)
                                                                    CTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCAC                        >  SRR4302543.4906734/1‑94 (MQ=60)
                                                                    CTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCAC                        >  SRR4302543.2531207/1‑94 (MQ=60)
                                                                    CTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCAC                        >  SRR4302543.1105360/1‑94 (MQ=60)
                                                                     TCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACA                       <  SRR4302543.4994513/94‑1 (MQ=60)
                                                                      CCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAG                      <  SRR4302543.935850/94‑1 (MQ=60)
                                                                      CCAGCACCCGGCGGATAAAACCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACAACAG                      >  SRR4302543.2560303/1‑94 (MQ=60)
                                                                       CAGCGCCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC                     <  SRR4302543.5789565/94‑1 (MQ=60)
                                                                       CAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC                     <  SRR4302543.7968746/94‑1 (MQ=60)
                                                                       CAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC                     <  SRR4302543.1984801/94‑1 (MQ=60)
                                                                       CAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC                     <  SRR4302543.4022787/94‑1 (MQ=60)
                                                                       CAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC                     <  SRR4302543.6799763/94‑1 (MQ=60)
                                                                       CAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC                     >  SRR4302543.6786598/1‑94 (MQ=60)
                                                                       CAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC                     <  SRR4302543.428319/94‑1 (MQ=60)
                                                                       CAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC                     <  SRR4302543.5925697/94‑1 (MQ=60)
                                                                        AGCACCCGGCGGATAAAATCCNCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCG                    <  SRR4302543.1073366/94‑1 (MQ=60)
                                                                        AGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCG                    <  SRR4302543.685784/94‑1 (MQ=60)
                                                                        AGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCG                    <  SRR4302543.189322/94‑1 (MQ=60)
                                                                        AGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCG                    <  SRR4302543.4048446/94‑1 (MQ=60)
                                                                         GCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGC                   <  SRR4302543.1676750/94‑1 (MQ=60)
                                                                          CACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCCTCGGCAAACCACAGCGCG                  >  SRR4302543.2823103/1‑94 (MQ=60)
                                                                          CACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCG                  >  SRR4302543.6217522/1‑94 (MQ=60)
                                                                          CACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCG                  <  SRR4302543.5331157/94‑1 (MQ=60)
                                                                            CCCGGCGGATAAAATCCGCGGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGG                <  SRR4302543.7569773/94‑1 (MQ=60)
                                                                            CCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGG                <  SRR4302543.5290399/94‑1 (MQ=60)
                                                                            CCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGG                >  SRR4302543.1811593/1‑94 (MQ=60)
                                                                            CCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGG                >  SRR4302543.3193059/1‑94 (MQ=60)
                                                                             CCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGC               >  SRR4302543.7401124/1‑94 (MQ=60)
                                                                              CGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCA              >  SRR4302543.4253021/1‑94 (MQ=60)
                                                                              CGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCA              >  SRR4302543.902660/1‑94 (MQ=60)
                                                                               GGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAG             >  SRR4302543.6859978/1‑94 (MQ=60)
                                                                                 CGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCA           <  SRR4302543.392961/94‑1 (MQ=60)
                                                                                  GGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCAC          >  SRR4302543.7890074/1‑94 (MQ=60)
                                                                                  GGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCAC          >  SRR4302543.7905977/1‑94 (MQ=60)
                                                                                  GGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCAC          >  SRR4302543.2793969/1‑94 (MQ=60)
                                                                                  GGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCAC          <  SRR4302543.2544178/94‑1 (MQ=60)
                                                                                  GGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCAC          >  SRR4302543.2183290/1‑94 (MQ=60)
                                                                                  GGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCAC          >  SRR4302543.4921921/1‑94 (MQ=60)
                                                                                   GATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACC         <  SRR4302543.3355060/94‑1 (MQ=60)
                                                                                       AAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTT     >  SRR4302543.7167637/1‑94 (MQ=60)
                                                                                        AATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTG    <  SRR4302543.3645148/94‑1 (MQ=60)
                                                                                        AATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTG    >  SRR4302543.2153203/1‑94 (MQ=60)
                                                                                          TCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCA  <  SRR4302543.2076598/94‑1 (MQ=60)
                                                                                             |                                                                                             
CCGGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCG‑AGGCGGCGCCGACGGGTTATCGGT‑‑CGCCG‑CGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCA  >  NC_000913/3553995‑3554177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑