Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,554,118 +C coding (367/396 nt) yhgKJ ← RNA phosphage cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,554,1171.C98.6% 438.6 / ‑1.1 143coding (368/396 nt)yhgKJRNA phosphage cyclase
Reads supporting (aligned to +/- strand):  ref base . (2/0);  new base C (57/84);  total (59/84)
Fisher's exact test for biased strand distribution p-value = 1.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01

Only 100 of 163 total aligned reads displayed.

GGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCG‑AGGCGGCGCCGAC‑GGGTTATCGGTCGCCG‑CGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGC  >  NC_000913/3554027‑3554199
                                                                                             |                                                                                  
ggTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGt‑gccg‑cc                                                                                  >  1:693952/1‑92 (MQ=255)
ggTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGt‑gccg‑cc                                                                                  >  2:3004909/1‑92 (MQ=255)
 gTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCCGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGGTATCGGt‑gccg‑ccg                                                                                 >  1:4096567/1‑91 (MQ=255)
 gTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCCGCGGATAAAATCCGCAAGCGGCGCCGAC‑GGGCTATCGGt‑gccg‑ccg                                                                                 >  2:4302840/1‑91 (MQ=255)
  tttGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGAAGGCGGCGCCGAC‑GGGTTATCGGt‑gccg‑ccgc                                                                                <  1:4251317/93‑4 (MQ=255)
   ttGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGt‑gccg‑ccgct                                                                               <  1:3119883/93‑5 (MQ=255)
      cTGATGAATTCCTTTTTCCGCCAGCAGCGGCTCCAGCGCCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCAc                                                                             <  1:4654931/93‑1 (MQ=255)
      cTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCAc                                                                             <  2:3195842/93‑1 (MQ=255)
      cTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCAc                                                                             <  1:221190/93‑1 (MQ=255)
       tgatgaATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACt                                                                            >  2:3313969/1‑93 (MQ=255)
       tgatgaATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACt                                                                            >  1:1687361/1‑93 (MQ=255)
       tgatgaATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACt                                                                            >  2:4180367/1‑93 (MQ=255)
        gatgaATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACtt                                                                           >  1:1256687/1‑93 (MQ=255)
        gatgaATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACtt                                                                           >  1:598811/1‑93 (MQ=255)
        gatgaATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGGTATCGGT‑GCCGCCGCTCACtt                                                                           >  1:4193217/1‑93 (MQ=255)
        gatgaATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGAGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACtt                                                                           >  2:4344219/1‑93 (MQ=255)
        gatgaATCCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACtt                                                                           <  1:890229/93‑1 (MQ=255)
         atgaATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTc                                                                          >  2:3220426/1‑93 (MQ=255)
           gaATTCCTATTTTCGCCAGCAGCGGCTCTAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCaa                                                                        >  1:1507279/1‑93 (MQ=255)
           gaATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCaa                                                                        <  2:1684647/93‑1 (MQ=255)
           gaATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCCCCGCC‑GGGTTATCGGT‑GCCGCCGCTCACTTCaa                                                                        >  1:663363/1‑93 (MQ=255)
            aaTTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAac                                                                       >  2:3833969/1‑93 (MQ=255)
             aTTCCTATTTTCGCGAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAaca                                                                      <  2:361236/93‑1 (MQ=255)
             aTTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGTTCCGC‑CGCTCACTTCAaca                                                                      >  1:3831162/1‑93 (MQ=255)
             aTTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAaca                                                                      >  2:714295/1‑93 (MQ=255)
             aTTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAaca                                                                      <  2:1890220/93‑1 (MQ=255)
              ttCCTATTTTCGCCAGCAGCGGCTCCAGCACCCCGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGGTATCGG‑CGCCGCCGCTCACTTCAacac                                                                     >  1:3076946/1‑93 (MQ=255)
                 cTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGGTATCGGT‑GCCGCCGCTGACTTCAACAcgcg                                                                  >  1:3791446/1‑93 (MQ=255)
                  tATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGa                                                                 <  1:2958011/93‑1 (MQ=255)
                  tATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGa                                                                 >  2:3935684/1‑93 (MQ=255)
                   aTTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGaa                                                                >  1:1889856/1‑93 (MQ=255)
                    ttttCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAg                                                               >  2:2285909/1‑93 (MQ=255)
                    ttttCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAg                                                               <  2:4380258/93‑1 (MQ=255)
                    tgttCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAg                                                               <  2:3368299/91‑1 (MQ=255)
                     tttCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAgg                                                              <  1:2760043/93‑1 (MQ=255)
                     tttCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAgg                                                              <  1:3353429/93‑1 (MQ=255)
                        caccAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTcc                                                           <  2:4598739/91‑1 (MQ=255)
                         cccAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCa                                                          <  2:2316461/92‑1 (MQ=255)
                          gcagcagCGGCGCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCAt                                                         <  1:786299/92‑1 (MQ=255)
                          ccAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCAt                                                         <  1:2457941/93‑1 (MQ=255)
                          ccAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCAt                                                         <  2:2732523/93‑1 (MQ=255)
                           cagcagCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGTGTCGC‑CGCTGACTTCAACACGCGAAGAGCCATc                                                        >  2:4547664/1‑93 (MQ=255)
                           cagcagCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATc                                                        <  2:2953682/93‑1 (MQ=255)
                           cagcagCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATc                                                        <  1:3583968/93‑1 (MQ=255)
                            agcagcGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCg                                                       <  1:3723252/93‑1 (MQ=255)
                             gcagcGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCgg                                                      >  2:2525845/1‑93 (MQ=255)
                             gcagcGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCgg                                                      >  1:3654856/1‑93 (MQ=255)
                               agcGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCa                                                    <  2:1774752/93‑1 (MQ=255)
                                gcGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCaa                                                   >  2:482215/1‑93 (MQ=255)
                                   gCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAAcc                                                >  2:1730319/1‑93 (MQ=255)
                                   gCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAAcc                                                <  2:119497/93‑1 (MQ=255)
                                   gCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAAcc                                                <  1:3937920/93‑1 (MQ=255)
                                    cTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCACACca                                               >  1:1112556/1‑93 (MQ=255)
                                     tCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACcac                                              <  2:2133212/93‑1 (MQ=255)
                                      ccAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAACGTCCATCGGCAAACcaca                                             >  1:2376299/1‑93 (MQ=255)
                                       cAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAg                                            <  1:1473037/93‑1 (MQ=255)
                                        aGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAgc                                           >  2:3403407/1‑93 (MQ=255)
                                         gCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAgcg                                          <  2:603072/93‑1 (MQ=255)
                                         gCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAgcg                                          <  1:3302346/93‑1 (MQ=255)
                                            cccGGCGGGAAAAATCCGCAGGCGGCGCCGGCGGGGTTAT‑GGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCgg                                       <  1:4493988/93‑1 (MQ=255)
                                             ccGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCggg                                      <  1:707844/93‑1 (MQ=255)
                                             ccGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCggg                                      >  1:3454541/1‑93 (MQ=255)
                                             ccGGCGGATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCggg                                      >  1:4540056/1‑93 (MQ=255)
                                              cggcggATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGgc                                     >  2:3299450/1‑93 (MQ=255)
                                               ggcggATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGgca                                    >  2:435680/1‑93 (MQ=255)
                                                gcggATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGgcag                                   >  2:821224/1‑93 (MQ=255)
                                                 cggATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGgcagc                                  >  2:1498242/1‑93 (MQ=255)
                                                  ggATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGTCCCGC‑CGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGgcagca                                 >  1:2954037/1‑93 (MQ=255)
                                                  ggATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGgcagca                                 >  2:1577049/1‑93 (MQ=255)
                                                   gATAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCAc                                >  2:308630/1‑93 (MQ=255)
                                                     tAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCg                              <  1:4048654/93‑1 (MQ=255)
                                                     tAAAATCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCg                              <  2:2241888/93‑1 (MQ=255)
                                                       aaaTCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGGAAACCACAGCGCGGGCCGCACCGtt                            >  2:249021/1‑93 (MQ=255)
                                                       aaaTCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAANCCACAGCGCGGGCAGCACCNtt                            >  1:3311862/1‑93 (MQ=255)
                                                       aaaTCCGCAGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGtt                            >  1:99594/1‑93 (MQ=255)
                                                             gcaGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTgcagc                      <  1:687445/91‑1 (MQ=255)
                                                              caGGCGGCGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTgcagca                     <  1:2901959/92‑1 (MQ=255)
                                                               cggcggcGCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCAc                    <  1:1358616/92‑1 (MQ=255)
                                                                  cggcgCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAg                 <  2:2428065/93‑1 (MQ=255)
                                                                  cggcgCCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAg                 <  1:2704207/93‑1 (MQ=255)
                                                                    gcgcCGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCg               <  1:4273601/93‑1 (MQ=255)
                                                                       ccGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTAc            >  2:2046265/1‑93 (MQ=255)
                                                                       ccGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTAc            <  1:680741/93‑1 (MQ=255)
                                                                       ccGAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTAc            <  1:2877747/93‑1 (MQ=255)
                                                                         gAC‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACaa          >  1:226068/1‑93 (MQ=255)
                                                                           c‑GGTTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACt        <  2:3556924/93‑1 (MQ=255)
                                                                           c‑GGGTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACt        <  1:1742458/93‑1 (MQ=255)
                                                                             gggTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACtt       <  1:874692/93‑1 (MQ=255)
                                                                              ggTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTc      >  2:3574666/1‑93 (MQ=255)
                                                                              ggTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTc      <  2:3776255/93‑1 (MQ=255)
                                                                              ggTGATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTc      <  1:3714123/93‑1 (MQ=255)
                                                                               gTTATCGGTTCCTC‑CGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTcc     <  2:3484842/93‑1 (MQ=255)
                                                                               gTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTcc     <  2:3493708/93‑1 (MQ=255)
                                                                               gTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTcc     <  2:2099889/93‑1 (MQ=255)
                                                                               gTTATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTcc     >  2:2789970/1‑93 (MQ=255)
                                                                                ttATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCg    <  1:2494374/93‑1 (MQ=255)
                                                                                ttATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCg    <  2:2383889/93‑1 (MQ=255)
                                                                                 tATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCgg   <  2:1879595/93‑1 (MQ=255)
                                                                                 tATCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCgg   <  2:2575326/93‑1 (MQ=255)
                                                                                  aTCGGT‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGgc  <  2:2532608/93‑1 (MQ=255)
                                                                                             |                                                                                  
GGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCG‑AGGCGGCGCCGAC‑GGGTTATCGGTCGCCG‑CGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGC  >  NC_000913/3554027‑3554199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCG‑AGGCGGCGCCGACGGGTTATCGGT‑‑CGCCG‑CGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGC  >  NC_000913/3554028‑3554211
                                                                                              |                                                                                             
GTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCACGCGGAGCCGACGGGGTATCGGTGGCGCCG‑C                                                                                               >  SRR4302535.2799551/1‑94 (MQ=60)
     CTGATGAATTCCTTTTTCCGCCAGCAGCGGCTCCAGCGCCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACT                                                                                        <  SRR4302535.4686438/94‑1 (MQ=60)
     CTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACT                                                                                        >  SRR4302535.1699575/1‑94 (MQ=60)
     CTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACT                                                                                        <  SRR4302535.222715/94‑1 (MQ=60)
      TGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTT                                                                                       >  SRR4302535.1265676/1‑94 (MQ=60)
      TGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTT                                                                                       >  SRR4302535.602997/1‑94 (MQ=60)
      TGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGGTATCGGT‑‑‑GCCGCCGCTCACTT                                                                                       >  SRR4302535.4221911/1‑94 (MQ=60)
       GATGAATCCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTC                                                                                      <  SRR4302535.896537/94‑1 (MQ=60)
        ATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCA                                                                                     <  SRR4302535.3865538/94‑1 (MQ=60)
         TGAATTCCTATTTTCGCCAGCAGCGGCTCTAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAA                                                                                    >  SRR4302535.1518115/1‑94 (MQ=60)
         TGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCCCCGCCGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAA                                                                                    >  SRR4302535.667998/1‑94 (MQ=60)
          GAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAAC                                                                                   <  SRR4302535.883003/94‑1 (MQ=60)
           AATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTTCCGC‑‑‑CGCTCACTTCAACA                                                                                  >  SRR4302535.3857581/1‑94 (MQ=60)
           AATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACA                                                                                  <  SRR4302535.2052591/94‑1 (MQ=60)
            ATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCCGCGGATAAAATCCGCAGGCGGCGCCGACGGGGTATCGG‑‑‑CGCCGCCGCTCACTTCAACAC                                                                                 >  SRR4302535.3098410/1‑94 (MQ=60)
               CCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCG                                                                              >  SRR4302535.2977012/1‑94 (MQ=60)
               CCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGGTATCGGT‑‑‑GCCGCCGCTGACTTCAACACGCG                                                                              >  SRR4302535.3817587/1‑94 (MQ=60)
                 TATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAA                                                                            >  SRR4302535.1903467/1‑94 (MQ=60)
                 TATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAA                                                                            <  SRR4302535.2978785/94‑1 (MQ=60)
                    TTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGT                                                                         <  SRR4302535.2779712/94‑1 (MQ=60)
                    TTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGT                                                                         <  SRR4302535.3376783/94‑1 (MQ=60)
                     TGCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTC                                                                        <  SRR4302535.4059028/94‑1 (MQ=60)
                        GCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCAT                                                                     <  SRR4302535.3462515/94‑1 (MQ=60)
                         GCAGCAGCGGCGCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATC                                                                    <  SRR4302535.791809/94‑1 (MQ=60)
                         CCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATC                                                                    <  SRR4302535.2475668/94‑1 (MQ=60)
                         CCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATC                                                                    <  SRR4302535.3750937/94‑1 (MQ=60)
                          CAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCG                                                                   <  SRR4302535.2287839/94‑1 (MQ=60)
                          CAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCG                                                                   <  SRR4302535.3608825/94‑1 (MQ=60)
                           AGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGG                                                                  <  SRR4302535.3749043/94‑1 (MQ=60)
                           AGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGG                                                                  >  SRR4302535.3680157/1‑94 (MQ=60)
                                 GGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACC                                                            <  SRR4302535.3154698/94‑1 (MQ=60)
                                  GCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCACACCA                                                           >  SRR4302535.1120493/1‑94 (MQ=60)
                                  GCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCA                                                           <  SRR4302535.3964793/94‑1 (MQ=60)
                                   CTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCAC                                                          <  SRR4302535.574021/94‑1 (MQ=60)
                                    TCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACA                                                         <  SRR4302535.700583/94‑1 (MQ=60)
                                    TCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAACGTCCATCGGCAAACCACA                                                         >  SRR4302535.2393369/1‑94 (MQ=60)
                                     CCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAG                                                        >  SRR4302535.2901090/1‑94 (MQ=60)
                                      CAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC                                                       <  SRR4302535.1483583/94‑1 (MQ=60)
                                      CAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC                                                       <  SRR4302535.674279/94‑1 (MQ=60)
                                      CAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGC                                                       <  SRR4302535.4679605/94‑1 (MQ=60)
                                        GCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGC                                                     <  SRR4302535.3325311/94‑1 (MQ=60)
                                         CACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCG                                                    <  SRR4302535.254423/94‑1 (MQ=60)
                                          CCCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGG                                                   <  SRR4302535.1328310/94‑1 (MQ=60)
                                          ACGCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGG                                                   <  SRR4302535.4195451/94‑1 (MQ=60)
                                           CCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGG                                                  >  SRR4302535.3478554/1‑94 (MQ=60)
                                           CCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGG                                                  >  SRR4302535.4571109/1‑94 (MQ=60)
                                            CCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGC                                                 <  SRR4302535.712811/94‑1 (MQ=60)
                                              cccggcgggaaaaatccgcaggcggcgccggcggggttatggtgccgcCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGG                                                  <  SRR4302535.4524834/46‑1 (MQ=60)
                                                ccgacAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCA                                             <  SRR4302535.4455539/89‑1 (MQ=60)
                                                CGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑CCCGC‑CGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCA                                             >  SRR4302535.2974788/1‑94 (MQ=60)
                                                CGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCA                                             >  SRR4302535.3649049/1‑94 (MQ=60)
                                                   ATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCG                                          >  SRR4302535.3708301/1‑94 (MQ=60)
                                                   ATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCG                                          <  SRR4302535.3357611/94‑1 (MQ=60)
                                                    TAAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGT                                         <  SRR4302535.4076366/94‑1 (MQ=60)
                                                     AAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAANCCACAGCGCGGGCAGCACCNTT                                        >  SRR4302535.3334908/1‑94 (MQ=60)
                                                     AAAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTT                                        >  SRR4302535.100294/1‑94 (MQ=60)
                                                      AAATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTT                                       <  SRR4302535.3103918/94‑1 (MQ=60)
                                                       AATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTG                                      >  SRR4302535.4161319/1‑94 (MQ=60)
                                                        ATCCGCAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGC                                     >  SRR4302535.4488454/1‑94 (MQ=60)
                                                            gcAGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCA                                 <  SRR4302535.692260/92‑1 (MQ=60)
                                                            C‑AGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCAC                                <  SRR4302535.2922473/94‑1 (MQ=60)
                                                              CGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACC                               <  SRR4302535.1368333/94‑1 (MQ=60)
                                                              AGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACC                               >  SRR4302535.2873666/1‑94 (MQ=60)
                                                              AGGCGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACC                               >  SRR4302535.1584149/1‑94 (MQ=60)
                                                                 CGGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGC                            <  SRR4302535.2723529/94‑1 (MQ=60)
                                                                 CGGCCCCTACGGGTTATCGGTTCCGC‑‑‑CGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGC                            <  SRR4302535.692111/94‑1 (MQ=60)
                                                                  GGCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCG                           <  SRR4302535.2497418/94‑1 (MQ=60)
                                                                   GCGCCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGT                          <  SRR4302535.4302855/94‑1 (MQ=60)
                                                                      CCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACA                       <  SRR4302535.2898126/94‑1 (MQ=60)
                                                                      CCGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACA                       <  SRR4302535.685507/94‑1 (MQ=60)
                                                                       CGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAA                      >  SRR4302535.4339576/1‑94 (MQ=60)
                                                                       CGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAA                      >  SRR4302535.227621/1‑94 (MQ=60)
                                                                       CGACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACACCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAC                      >  SRR4302535.4241591/1‑94 (MQ=60)
                                                                         ACGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACT                    <  SRR4302535.1782873/94‑1 (MQ=60)
                                                                          CGGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTT                   <  SRR4302535.1755057/94‑1 (MQ=60)
                                                                           GGGTTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTC                  <  SRR4302535.880890/94‑1 (MQ=60)
                                                                              TTATCGGT‑‑‑GCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGG               <  SRR4302535.2512372/94‑1 (MQ=60)
                                                                               ggtgatcggtgccgcCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCC                 <  SRR4302535.3739854/79‑1 (MQ=60)
                                                                                      ggtgccgcCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTA          >  SRR4302535.3401654/9‑94 (MQ=60)
                                                                                       gtgccgcCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTAC         <  SRR4302535.4514387/87‑1 (MQ=60)
                                                                                          CCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGA      >  SRR4302535.542400/1‑94 (MQ=60)
                                                                                           cgcCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGAT     >  SRR4302535.3459665/4‑94 (MQ=60)
                                                                                            gcCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATA    <  SRR4302535.1908078/92‑1 (MQ=60)
                                                                                            gcCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATA    <  SRR4302535.805252/92‑1 (MQ=60)
                                                                                            C‑CGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAG   <  SRR4302535.4702183/94‑1 (MQ=60)
                                                                                              CGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGC  >  SRR4302535.784089/1‑94 (MQ=60)
                                                                                              CGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGAGAGC  >  SRR4302535.268625/1‑94 (MQ=60)
                                                                                              |                                                                                             
GTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCG‑AGGCGGCGCCGACGGGTTATCGGT‑‑CGCCG‑CGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGC  >  NC_000913/3554028‑3554211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑