Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,582,668 G→A L93L (TTG→TTA)  yrhB → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,582,6680GA97.5% 551.4 / NA 162L93L (TTG→TTA) yrhBhypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (127/31);  minor base C (0/4);  total (127/35)
Fisher's exact test for biased strand distribution p-value = 1.89e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 179 total aligned reads displayed.

AGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATG  >  NC_000913/3582578‑3582755
                                                                                          |                                                                                       
aGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTAcc                                                                                       >  2:2420880/1‑93 (MQ=255)
aGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTAcc                                                                                       >  1:1147085/1‑93 (MQ=255)
 gACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTAccc                                                                                      >  2:3007630/1‑93 (MQ=255)
 gACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTAccc                                                                                      >  2:2839968/1‑93 (MQ=255)
  aCAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTATCTTCGGCTTACCCt                                                                                     >  2:3960764/1‑93 (MQ=255)
  aCAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCt                                                                                     >  1:2166628/1‑93 (MQ=255)
   cAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTg                                                                                    >  2:2894263/1‑93 (MQ=255)
    aGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGa                                                                                   <  1:932069/93‑1 (MQ=255)
     gtggtgAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGAc                                                                                  >  2:2229620/1‑93 (MQ=255)
     gtggtgAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGAc                                                                                  <  1:3152351/93‑1 (MQ=255)
      tggtgAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACa                                                                                 >  1:2438028/1‑93 (MQ=255)
      tggtgAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACa                                                                                 >  2:3567310/1‑93 (MQ=255)
       ggtgAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACaa                                                                                <  1:4493386/93‑1 (MQ=255)
        gtgAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACaaa                                                                               <  2:655268/93‑1 (MQ=255)
          gAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAAt                                                                             >  1:2274295/1‑93 (MQ=255)
           aaaTTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATa                                                                            >  2:2054993/1‑93 (MQ=255)
           aaaTTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATa                                                                            <  2:754548/93‑1 (MQ=255)
           aaaTTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATa                                                                            >  1:3500175/1‑93 (MQ=255)
            aaTTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATac                                                                           >  1:486719/1‑92 (MQ=255)
            aaTTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAg                                                                           <  1:1595363/93‑1 (MQ=255)
             attcattcTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGc                                                                          >  1:1615259/1‑93 (MQ=255)
              ttcattcTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGcc                                                                         >  1:576442/1‑93 (MQ=255)
                cattcTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCct                                                                       <  2:4356118/93‑1 (MQ=255)
                  ttcTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCctct                                                                     >  1:795842/1‑93 (MQ=255)
                  ttcTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCctct                                                                     >  1:1162468/1‑93 (MQ=255)
                   tctcTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCtt                                                                    <  1:3906337/93‑1 (MQ=255)
                     tctGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTcc                                                                  >  2:334092/1‑93 (MQ=255)
                     tctGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTcc                                                                  >  1:2151285/1‑93 (MQ=255)
                     tctGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTcc                                                                  >  2:2515118/1‑93 (MQ=255)
                          gAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGa                                                             <  1:513816/93‑1 (MQ=255)
                          gAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGa                                                             >  1:4542699/1‑93 (MQ=255)
                            aCGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAag                                                           >  2:477288/1‑93 (MQ=255)
                             cGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAaga                                                          <  1:3935345/93‑1 (MQ=255)
                              ggCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAagag                                                         <  1:2344348/93‑1 (MQ=255)
                              ggCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAagag                                                         >  1:3324246/1‑93 (MQ=255)
                              ggCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAagag                                                         <  2:4386686/93‑1 (MQ=255)
                              ggCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAagag                                                         >  2:3423967/1‑93 (MQ=255)
                              ggCAAAACCGCTGAAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAagag                                                         <  2:1228430/93‑1 (MQ=255)
                                 aaaaCCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGc                                                      <  2:264604/93‑1 (MQ=255)
                                 aaaaCCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGc                                                      <  2:741915/93‑1 (MQ=255)
                                         tGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAAcc                                              >  1:1849867/1‑93 (MQ=255)
                                          ggAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCt                                             >  2:4112234/1‑93 (MQ=255)
                                          ggAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCt                                             >  2:101876/1‑93 (MQ=255)
                                            aagaaTATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTa                                           <  1:3005379/93‑1 (MQ=255)
                                             agaaTATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAg                                          >  2:4655890/1‑93 (MQ=255)
                                             agaaTATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAg                                          <  1:3563341/93‑1 (MQ=255)
                                              gaaTATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAga                                         >  2:1047404/1‑92 (MQ=255)
                                              gaaTATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGt                                         >  2:4436489/1‑93 (MQ=255)
                                              gaaTATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGt                                         >  1:354396/1‑93 (MQ=255)
                                               aaTATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTa                                        >  2:3865589/1‑93 (MQ=255)
                                               aaTATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGACCTCTTCCCACGAAGAGGGCCGCTAACCTTCGTa                                        >  2:2339799/1‑93 (MQ=255)
                                                atatCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTAc                                       <  1:3023991/93‑1 (MQ=255)
                                                 tatCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTAcc                                      >  1:1408850/1‑93 (MQ=255)
                                                     tACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCccgc                                  >  1:1632367/1‑93 (MQ=255)
                                                     tACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACACTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCccgc                                  >  2:2735683/1‑93 (MQ=255)
                                                       cAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCccgccg                                >  1:4416118/1‑93 (MQ=255)
                                                        aGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGt                               >  2:1980991/1‑93 (MQ=255)
                                                         ggATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGtt                              >  1:901/1‑93 (MQ=255)
                                                         ggATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGtt                              >  2:1117541/1‑93 (MQ=255)
                                                         ggATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGtt                              >  2:2027096/1‑93 (MQ=255)
                                                            ttACGAAATAAAAAACGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGCGCCGCTAACCTTAGTACCCCGCCGtttat                           >  2:914282/1‑90 (MQ=255)
                                                             tACGAAAAAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAat                          <  1:4293997/93‑1 (MQ=255)
                                                              acaaaaaaaaaaaGGCTCCCTTCGGTTTCCCCTGACAAAATACCCCTCTCCCCACGAAGAGGGCCGCTAACCTTAGTCCCCCGCCGTTAAatc                         <  2:3479802/90‑1 (MQ=255)
                                                              aCGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAatc                         >  1:4237329/1‑93 (MQ=255)
                                                               caaaaTAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTCCCCCGCCGTTAAatca                        <  2:2149623/91‑1 (MQ=255)
                                                               aaaaaaaaaaaaGCCTCCCTTCGCTTTCCCTTAAAAAAATACCCCTCTCCCCACAAAGAGGGCCGCTAACCTTAGTCCCCCGCCGTTAAatca                        <  2:3187203/91‑1 (MQ=255)
                                                                gAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAatcat                       >  1:2666509/1‑93 (MQ=255)
                                                                aaaaTAAAAAAGTCTCCCTTGGGCTTACCCGGACAAAATAGCCCTCTCCCCACGAAGAGGGCCGCTACCCTTAGTCCCCCGCCGTTAAatcat                       <  2:1939811/92‑1 (MQ=255)
                                                                 aaaTAAAAAAGGCTCCCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTCCCCCGCCGTTAAatcatc                      <  2:745606/93‑1 (MQ=255)
                                                                 aaaTAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAatcatc                      >  1:806981/1‑93 (MQ=255)
                                                                 aaaTAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCAGCTAACCTTAGTACCCCGCCGTTAAatcatg                      >  2:2520346/1‑92 (MQ=255)
                                                                  aaTAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTCCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATcc                     <  1:2904338/93‑1 (MQ=255)
                                                                   aTAAAAAAGGCTCCCTTCGGCTTCCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTCCCCCGCCGTTAAATCATCCa                    <  1:1461515/93‑1 (MQ=255)
                                                                   aTAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCa                    >  2:3580508/1‑93 (MQ=255)
                                                                    aaaaaaaGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTCCCCCGCCGTTAAATCATCCAc                   <  2:1482145/92‑1 (MQ=255)
                                                                     aaaaaaGGCTACCTTCGGCTTACCCTGACAAAATAGCCTTCTTCCCATGAAAAGGGCCCCTAACCTTATTACCCCGCCGTTAAATCATCCAcc                  >  2:1442543/1‑93 (MQ=255)
                                                                     aaaaaaGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCAcc                  >  1:3179428/1‑93 (MQ=255)
                                                                       aaaaGGCTACCTTCGGCTTCCCCTGACAAAATAGCCCTCTTCCCACGAAGCGGGCCGCTAACCTTAGTCCCCCGCCGTTAAATCATCCACCGa                <  1:3302944/93‑1 (MQ=255)
                                                                        aaaGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAg               >  2:2588005/1‑93 (MQ=255)
                                                                         aaGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAgc              >  1:373440/1‑93 (MQ=255)
                                                                         aaGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAgc              >  2:3693106/1‑93 (MQ=255)
                                                                         aaGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAgc              >  1:4006353/1‑93 (MQ=255)
                                                                          aGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAgcg             >  2:1755749/1‑93 (MQ=255)
                                                                          aGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAgcg             >  2:839501/1‑93 (MQ=255)
                                                                          aGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAgcg             >  1:4599918/1‑93 (MQ=255)
                                                                          aGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAgcg             >  2:4608702/1‑93 (MQ=255)
                                                                           ggCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTCCCCCGCCGTTAAATCATCCACCGAgcgc            <  1:3850364/93‑1 (MQ=255)
                                                                           ggCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAgcgc            >  2:4057750/1‑93 (MQ=255)
                                                                           ggCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAgcgc            >  1:3408565/1‑93 (MQ=255)
                                                                             cgaCCTTCGGCTTCCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTACCCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCgg          <  2:1364068/91‑1 (MQ=255)
                                                                              tACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCggg         >  1:2582060/1‑93 (MQ=255)
                                                                              tACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCggg         >  1:3100698/1‑93 (MQ=255)
                                                                               cccTTGGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTCCCCCGCCGTTAAATCATCCACCGAGCGCGGGt        <  2:1327908/92‑1 (MQ=255)
                                                                               aCCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAGTCATCCACCGAGCGCGGGt        >  1:2655951/1‑93 (MQ=255)
                                                                                 cTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCg      >  2:4182771/1‑93 (MQ=255)
                                                                                 cTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCg      >  1:2251047/1‑93 (MQ=255)
                                                                                 cTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCg      >  1:2009387/1‑93 (MQ=255)
                                                                                  ttCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCgg     >  1:44907/1‑93 (MQ=255)
                                                                                  ttCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCgg     >  2:1486556/1‑93 (MQ=255)
                                                                                   tCGCCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTCCCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGa    <  2:2382018/93‑1 (MQ=255)
                                                                                          |                                                                                       
AGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATG  >  NC_000913/3582578‑3582755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAAAGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCG  >  NC_000913/3582575‑3582760
                                                                                             |                                                                                            
TAAAGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTA                                                                                              >  SRR4302535.4579725/1‑94 (MQ=60)
TAAAGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTA                                                                                              >  SRR4302535.1378834/1‑94 (MQ=60)
 AAAGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTAC                                                                                             >  SRR4302535.618989/1‑94 (MQ=60)
 AAAGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTAC                                                                                             >  SRR4302535.3601799/1‑94 (MQ=60)
  AAGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACC                                                                                            >  SRR4302535.1155258/1‑94 (MQ=60)
   AGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCC                                                                                           >  SRR4302535.456615/1‑94 (MQ=60)
    GACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCT                                                                                          >  SRR4302535.2182159/1‑94 (MQ=60)
      CAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGA                                                                                        >  SRR4302535.4608457/1‑94 (MQ=60)
      CAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGA                                                                                        >  SRR4302535.1776369/1‑94 (MQ=60)
       AGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGAC                                                                                       >  SRR4302535.1629720/1‑94 (MQ=60)
       AGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGAC                                                                                       <  SRR4302535.938659/94‑1 (MQ=60)
        GTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACA                                                                                      <  SRR4302535.3174257/94‑1 (MQ=60)
        GTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACA                                                                                      >  SRR4302535.2455589/1‑94 (MQ=60)
          GGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAA                                                                                    <  SRR4302535.4524229/94‑1 (MQ=60)
            TGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAAT                                                                                  >  SRR4302535.2290528/1‑94 (MQ=60)
             GAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATA                                                                                 >  SRR4302535.3524509/1‑94 (MQ=60)
              AAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAC                                                                                >  SRR4302535.490131/1‑94 (MQ=60)
               AATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGC                                                                               <  SRR4302535.1606869/94‑1 (MQ=60)
               AATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGC                                                                               >  SRR4302535.1626901/1‑94 (MQ=60)
                ATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCC                                                                              >  SRR4302535.2871245/1‑94 (MQ=60)
                ATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCC                                                                              >  SRR4302535.580492/1‑94 (MQ=60)
                  TCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCT                                                                            <  SRR4302535.3954170/94‑1 (MQ=60)
                   CATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTC                                                                           >  SRR4302535.336330/1‑94 (MQ=60)
                    ATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCT                                                                          >  SRR4302535.1170776/1‑94 (MQ=60)
                    ATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCT                                                                          >  SRR4302535.801428/1‑94 (MQ=60)
                      TCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTC                                                                        <  SRR4302535.3933100/94‑1 (MQ=60)
                       CTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCC                                                                       >  SRR4302535.2166750/1‑94 (MQ=60)
                        TCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCC                                                                      >  SRR4302535.2267498/1‑94 (MQ=60)
                        TCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCC                                                                      >  SRR4302535.4292169/1‑94 (MQ=60)
                         CTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCA                                                                     >  SRR4302535.689200/1‑94 (MQ=60)
                            GGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGA                                                                  >  SRR4302535.4573770/1‑94 (MQ=60)
                             GAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAA                                                                 <  SRR4302535.517405/94‑1 (MQ=60)
                                CGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAG                                                              >  SRR4302535.3562746/1‑94 (MQ=60)
                                CGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAG                                                              <  SRR4302535.472569/94‑1 (MQ=60)
                                CGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAG                                                              >  SRR4302535.3347376/1‑94 (MQ=60)
                                CGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAG                                                              <  SRR4302535.3962196/94‑1 (MQ=60)
                                 GGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGG                                                             <  SRR4302535.2361142/94‑1 (MQ=60)
                                   CAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGC                                                           <  SRR4302535.4471415/94‑1 (MQ=60)
                                    AAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCC                                                          >  SRR4302535.4441128/1‑94 (MQ=60)
                                      AACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGC                                                        >  SRR4302535.1529827/1‑94 (MQ=60)
                                       ACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCT                                                       >  SRR4302535.534017/1‑94 (MQ=60)
                                           CTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACC                                                   >  SRR4302535.1863199/1‑94 (MQ=60)
                                               AAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAG                                               <  SRR4302535.3026441/94‑1 (MQ=60)
                                                AGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGT                                              >  SRR4302535.356843/1‑94 (MQ=60)
                                                AGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGT                                              <  SRR4302535.3588048/94‑1 (MQ=60)
                                                   ATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACC                                           >  SRR4302535.1418912/1‑94 (MQ=60)
                                                   ATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACC                                           >  SRR4302535.3090046/1‑94 (MQ=60)
                                                   ATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACC                                           <  SRR4302535.3045162/94‑1 (MQ=60)
                                                     ATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCC                                         >  SRR4302535.1179299/1‑94 (MQ=60)
                                                       CTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGC                                       >  SRR4302535.3149150/1‑94 (MQ=60)
                                                       CTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGC                                       >  SRR4302535.1644163/1‑94 (MQ=60)
                                                         ACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCG                                     >  SRR4302535.4446346/1‑94 (MQ=60)
                                                         ACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCG                                     >  SRR4302535.802672/1‑94 (MQ=60)
                                                          CAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGT                                    >  SRR4302535.298378/1‑94 (MQ=60)
                                                           AGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTT                                   >  SRR4302535.912/1‑94 (MQ=60)
                                                           AGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTT                                   >  SRR4302535.2187626/1‑94 (MQ=60)
                                                             GATTACGAAAAAAAAAAGCTTACCTTCGGCTTACCCTGACAAAATACCCCCCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAA                                 <  SRR4302535.1457356/94‑1 (MQ=60)
                                                             GATAACAAAATAAAAAAGGCTCCCTTCGGCTTCCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAA                                 <  SRR4302535.3333354/94‑1 (MQ=60)
                                                                TACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATC                              >  SRR4302535.4266325/1‑94 (MQ=60)
                                                                TACGAAAAAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATC                              <  SRR4302535.4323377/94‑1 (MQ=60)
                                                                  CGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCAT                            >  SRR4302535.2685597/1‑94 (MQ=60)
                                                                   GAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATC                           >  SRR4302535.812639/1‑94 (MQ=60)
                                                                     AATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTCCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCA                         <  SRR4302535.2924870/94‑1 (MQ=60)
                                                                      ATAAAAAAGGCTCCCTTCGGCTTCCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTCCCCCGCCGTTAAATCATCCAC                        <  SRR4302535.1471987/94‑1 (MQ=60)
                                                                      ATAAAAAAGGCTCCCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTCCCCCGCCGTTAAATCATCCAC                        <  SRR4302535.1471986/94‑1 (MQ=60)
                                                                      ATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCAC                        >  SRR4302535.1961551/1‑94 (MQ=60)
                                                                       TAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACC                       >  SRR4302535.3201410/1‑94 (MQ=60)
                                                                       AAAAAAAGGCTCCCTTCGGCTTCCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTCCCCCGCCGTTAAATCATCCACC                       <  SRR4302535.4079894/94‑1 (MQ=60)
                                                                         AAAAAGGCTACCTTCGGCTTCCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTCCCCCGCCGTTAAATCATCCACCGA                     <  SRR4302535.3192153/94‑1 (MQ=60)
                                                                          AAAAGGCTACCTTCGGCTTCCCCTGACAAAATAGCCCTCTTCCCACGAAGCGGGCCGCTAACCTTAGTCCCCCGCCGTTAAATCATCCACCGAG                    <  SRR4302535.3325912/94‑1 (MQ=60)
                                                                           AAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGC                   >  SRR4302535.4033757/1‑94 (MQ=60)
                                                                           AAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGC                   >  SRR4302535.3802233/1‑94 (MQ=60)
                                                                           AAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGC                   >  SRR4302535.376024/1‑94 (MQ=60)
                                                                            AAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCG                  >  SRR4302535.4631204/1‑94 (MQ=60)
                                                                             AGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGC                 >  SRR4302535.3432290/1‑94 (MQ=60)
                                                                              GGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTCCCCCGCCGTTAAATCATCCACCGAGCGCG                <  SRR4302535.3876860/94‑1 (MQ=60)
                                                                                CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGG              >  SRR4302535.3122284/1‑94 (MQ=60)
                                                                                CTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGG              >  SRR4302535.2600609/1‑94 (MQ=60)
                                                                                 TACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAGGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGT             >  SRR4302535.2674981/1‑94 (MQ=60)
                                                                                 TACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAGTCATCCACCGAGCGCGGGT             >  SRR4302535.2674980/1‑94 (MQ=60)
                                                                                   CCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCG           >  SRR4302535.3897449/1‑94 (MQ=60)
                                                                                   CCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCG           >  SRR4302535.964806/1‑94 (MQ=60)
                                                                                   CCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCG           >  SRR4302535.2023855/1‑94 (MQ=60)
                                                                                   CCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCG           >  SRR4302535.2034356/1‑94 (MQ=60)
                                                                                   CCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCG           >  SRR4302535.2267079/1‑94 (MQ=60)
                                                                                    CTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGG          >  SRR4302535.2629405/1‑94 (MQ=60)
                                                                                    CTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGG          >  SRR4302535.3505953/1‑94 (MQ=60)
                                                                                    CTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGG          >  SRR4302535.45245/1‑94 (MQ=60)
                                                                                      TGGGCTTACCCTGACAAAATAGCCCTCTCCCCACGAAGAGGGCCCCTAACCTTAGTCCCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGAT        <  SRR4302535.2338029/94‑1 (MQ=60)
                                                                                      TCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTTGGAT        >  SRR4302535.2389217/1‑94 (MQ=60)
                                                                                        GGCTTCCCCTGACAAAATAGCCCTCTCCCCACGAAGAGGGCCGCTACCCTTAGTCCCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGC      <  SRR4302535.3720198/94‑1 (MQ=60)
                                                                                         GCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATACAACAAGCGCGGGACGGATGGG     >  SRR4302535.844669/1‑94 (MQ=60)
                                                                                          CTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGC    >  SRR4302535.4656516/1‑94 (MQ=60)
                                                                                          CTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGC    >  SRR4302535.1059781/1‑94 (MQ=60)
                                                                                          CTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGC    >  SRR4302535.1683359/1‑94 (MQ=60)
                                                                                           TTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCC   >  SRR4302535.301643/1‑94 (MQ=60)
                                                                                            TCCCCTGACAAAATAGCCCTCTCCCCACGAAGAGGGCCGCTACCCTTGGTCCCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCG  <  SRR4302535.2343891/94‑1 (MQ=60)
                                                                                             |                                                                                            
TAAAGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCG  >  NC_000913/3582575‑3582760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑