Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,597,072	T→C	D73G (GAC→GGC)	b3460 ←	LivJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,597,072	0	T	C	99.6%	870.8 / ‑5.0	274	D73G (GAC→GGC)	b3460	LivJ
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (154/119); total (155/119)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01

Only 100 of 276 total aligned reads displayed.

GAAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGG  >  NC_000913/3596982‑3597161

   gaaCAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCatat                                                                                      <  2:712330/93‑1 (MQ=255)

    aaCAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATAtt                                                                                     <  1:1215933/93‑1 (MQ=255)

    aaCAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATAtt                                                                                     <  1:319205/93‑1 (MQ=255)

     aCAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATAttt                                                                                    >  1:2721492/1‑93 (MQ=255)

     aCAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACAGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATAttt                                                                                    >  2:1566796/1‑93 (MQ=255)

       agagGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTa                                                                                  >  2:750153/1‑93 (MQ=255)

       agagGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTa                                                                                  >  2:2407740/1‑93 (MQ=255)

       agagGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTa                                                                                  <  1:3440741/93‑1 (MQ=255)

        gagGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTAc                                                                                 >  2:1655134/1‑93 (MQ=255)

          ggTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACgt                                                                               >  2:2923817/1‑92 (MQ=255)

          ggTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGa                                                                               <  1:1970698/93‑1 (MQ=255)

             gACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGAttt                                                                            <  2:1087309/93‑1 (MQ=255)

             gACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGAttt                                                                            <  1:558552/93‑1 (MQ=255)

             gACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGACACAAGCATCGCCATATTTTACGAttt                                                                            >  2:1540370/1‑93 (MQ=255)

               ccAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGc                                                                          >  1:2794111/1‑93 (MQ=255)

                cAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCa                                                                         <  1:1007301/93‑1 (MQ=255)

                cAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCa                                                                         >  2:581957/1‑93 (MQ=255)

                cAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCa                                                                         >  2:4163927/1‑93 (MQ=255)

                 aaTCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCCACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAg                                                                        >  2:3783313/1‑93 (MQ=255)

                 aaTCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAg                                                                        >  1:4593903/1‑93 (MQ=255)

                 aaTCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAg                                                                        >  2:1753443/1‑93 (MQ=255)

                 aaTCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAg                                                                        <  2:4042342/93‑1 (MQ=255)

                  aTCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGt                                                                       >  2:3726348/1‑93 (MQ=255)

                   tCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGtt                                                                      <  1:2521289/93‑1 (MQ=255)

                   tCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGtt                                                                      >  2:4096270/1‑93 (MQ=255)

                    cacaTATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGttt                                                                     <  2:11451/93‑1 (MQ=255)

                     acaTATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTttg                                                                    >  2:882618/1‑93 (MQ=255)

                     acaTATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTttg                                                                    >  1:2020022/1‑93 (MQ=255)

                       atatTTCATTCCGTCGTTAACGACATTGAACACATCCGCAAACGCCTATTTAGAGACACAGGCATCGCCATAAAATACGATTTGCAGTatgtt                                                                  <  2:4686637/93‑1 (MQ=255)

                       atatTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTttgtt                                                                  <  1:2231607/93‑1 (MQ=255)

                        tatTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTttgttg                                                                 >  2:1296103/1‑93 (MQ=255)

                        tatTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTttgttg                                                                 >  1:3823973/1‑93 (MQ=255)

                          tttAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGcc                                                               >  2:3140971/1‑93 (MQ=255)

                            tAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCtt                                                             >  1:2342785/1‑93 (MQ=255)

                             aaTGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCttt                                                            >  1:4431846/1‑93 (MQ=255)

                             aaTGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCttt                                                            >  1:2161068/1‑93 (MQ=255)

                              aTGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTa                                                           >  1:3512308/1‑93 (MQ=255)

                               tGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTaa                                                          <  2:2173112/93‑1 (MQ=255)

                               tGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTaa                                                          <  1:2408132/93‑1 (MQ=255)

                               tGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTaa                                                          <  1:2906799/93‑1 (MQ=255)

                               tGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTaa                                                          <  2:1583879/93‑1 (MQ=255)

                                gCCGTCGTTAACGACTTTGTTCGCCACCGCAACCTCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAAt                                                         <  1:627102/93‑1 (MQ=255)

                                gCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAAt                                                         >  2:1189244/1‑93 (MQ=255)

                                gCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAAt                                                         <  1:4543835/93‑1 (MQ=255)

                                 ccGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATg                                                        <  2:3386975/93‑1 (MQ=255)

                                  cgtcgtTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATgc                                                       >  1:932973/1‑93 (MQ=255)

                                   gtcgtTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATgcc                                                      <  1:4272746/93‑1 (MQ=255)

                                   gtcgtTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATgcc                                                      >  2:1207194/1‑93 (MQ=255)

                                     cgtTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATgccgc                                                    >  2:2005657/1‑93 (MQ=255)

                                      gtTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATgccgcc                                                   <  2:4272601/93‑1 (MQ=255)

                                            taCTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGc                                             <  1:3752259/92‑1 (MQ=255)

                                            gACTTTGTTCGCCACCGCACCCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGc                                             >  2:4568043/1‑93 (MQ=255)

                                             aCTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCg                                            >  2:2504082/1‑93 (MQ=255)

                                             aCTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCg                                            >  2:3035588/1‑93 (MQ=255)

                                              cTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGt                                           >  2:911444/1‑93 (MQ=255)

                                              cTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGt                                           >  2:2205368/1‑93 (MQ=255)

                                              cTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGt                                           <  1:720843/93‑1 (MQ=255)

                                               tttGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGtt                                          >  1:1602486/1‑93 (MQ=255)

                                               tttGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGtt                                          <  2:4080301/93‑1 (MQ=255)

                                                 tGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGa                                        <  1:4106942/93‑1 (MQ=255)

                                                  gTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGat                                       <  1:1473484/93‑1 (MQ=255)

                                                   ttCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGata                                      <  1:4390908/93‑1 (MQ=255)

                                                   ttCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGata                                      <  2:4455757/93‑1 (MQ=255)

                                                    tCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGatat                                     <  2:3828950/93‑1 (MQ=255)

                                                    tCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGatat                                     <  2:3250331/93‑1 (MQ=255)

                                                     cGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATc                                    <  2:2662110/93‑1 (MQ=255)

                                                      gCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATcc                                   <  1:3770361/93‑1 (MQ=255)

                                                         aCCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCa                                >  1:2945024/1‑93 (MQ=255)

                                                         aCCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCa                                >  1:3103438/1‑93 (MQ=255)

                                                         aCCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCa                                >  2:3082065/1‑93 (MQ=255)

                                                          ccGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCaa                               <  2:3670995/93‑1 (MQ=255)

                                                          ccGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCaa                               >  1:4130790/1‑93 (MQ=255)

                                                           cGCAACCGCCTGTTTCGTGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAAc                              <  2:2795660/93‑1 (MQ=255)

                                                            gCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAAcc                             >  1:1381248/1‑93 (MQ=255)

                                                             cAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCg                            >  1:1303747/1‑93 (MQ=255)

                                                               aCCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGcc                          <  1:3393881/93‑1 (MQ=255)

                                                               aCCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGcc                          >  2:4500668/1‑93 (MQ=255)

                                                                  gCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGc                       <  1:1022972/93‑1 (MQ=255)

                                                                  gCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGc                       >  1:3254411/1‑93 (MQ=255)

                                                                    cTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGctc                     >  1:1725681/1‑93 (MQ=255)

                                                                    cTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGctc                     >  1:1678250/1‑93 (MQ=255)

                                                                    cTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGctc                     >  2:816551/1‑93 (MQ=255)

                                                                    cTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGctc                     <  1:1555163/93‑1 (MQ=255)

                                                                    cTGTTTCGGGACACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGACGCCTTTAGCGTTGATATCAGAAACCGCCTGctc                     >  2:1420652/1‑93 (MQ=255)

                                                                     tGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGctct                    >  1:2837485/1‑93 (MQ=255)

                                                                     tGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGctct                    >  2:3285560/1‑93 (MQ=255)

                                                                       tttCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTgc                  >  2:1493240/1‑93 (MQ=255)

                                                                          cGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGcc               >  1:1141021/1‑93 (MQ=255)

                                                                             gTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGt            <  2:524351/93‑1 (MQ=255)

                                                                              tCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTa           >  1:369805/1‑93 (MQ=255)

                                                                                acaGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTaaa         >  2:1889217/1‑93 (MQ=255)

                                                                                 caGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAAc        <  2:2830142/93‑1 (MQ=255)

                                                                                   ggCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTc      >  1:3937274/1‑93 (MQ=255)

                                                                                      aTCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTg   <  1:431031/93‑1 (MQ=255)

                                                                                      aTCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTg   >  1:1299446/1‑93 (MQ=255)

                                                                                      aTCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTg   <  2:3516995/93‑1 (MQ=255)

                                                                                       tCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACTGCCTGCTCTGCGACGGTAAACTCCTgg  >  1:1452135/1‑93 (MQ=255)

                                                                                       tCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTgg  >  2:4365724/1‑93 (MQ=255)

                                                                                       tCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTgg  >  1:1660319/1‑93 (MQ=255)

                                                                                       tCGCCATATTTTACGATTTGCAGTTTGTTGCCGTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTgg  >  2:698004/1‑93 (MQ=255)

GAAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGG  >  NC_000913/3596982‑3597161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GATGAAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGGTCAC  >  NC_000913/3596979‑3597165

GATGAAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGC                                                                                               >  SRR4302535.3315851/1‑94 (MQ=60)

 ATGAAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCC                                                                                              >  SRR4302535.571703/1‑94 (MQ=60)

   GAAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCAT                                                                                            >  SRR4302535.1468995/1‑94 (MQ=60)

   GAAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCAT                                                                                            <  SRR4302535.3647177/94‑1 (MQ=60)

     AGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATAT                                                                                          >  SRR4302535.1354334/1‑94 (MQ=60)

       AACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGGCTTTGTTCGCCACCGCACCCGCCTGTTTCGGGTCACAGGCATCGCCATATTT                                                                                        <  SRR4302535.2003866/94‑1 (MQ=60)

       AACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTT                                                                                        >  SRR4302535.1024045/1‑94 (MQ=60)

       AACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTT                                                                                        >  SRR4302535.2740931/1‑94 (MQ=60)

       AACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTT                                                                                        >  SRR4302535.2747836/1‑94 (MQ=60)

       AACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTT                                                                                        <  SRR4302535.1224655/94‑1 (MQ=60)

       AACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTT                                                                                        <  SRR4302535.321413/94‑1 (MQ=60)

        ACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTT                                                                                       >  SRR4302535.3500478/1‑94 (MQ=60)

        ACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTT                                                                                       >  SRR4302535.791495/1‑94 (MQ=60)

         CAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTA                                                                                      >  SRR4302535.2444735/1‑94 (MQ=60)

          AGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTAC                                                                                     <  SRR4302535.3464669/94‑1 (MQ=60)

            AGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGA                                                                                   >  SRR4302535.1954310/1‑94 (MQ=60)

             GGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGAT                                                                                  <  SRR4302535.1984892/94‑1 (MQ=60)

             GGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGAT                                                                                  >  SRR4302535.30855/1‑94 (MQ=60)

              GTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATT                                                                                 <  SRR4302535.3385257/94‑1 (MQ=60)

                GACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTG                                                                               <  SRR4302535.562457/94‑1 (MQ=60)

                 ACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGC                                                                              >  SRR4302535.1448954/1‑94 (MQ=60)

                 ACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGC                                                                              >  SRR4302535.2813991/1‑94 (MQ=60)

                  CCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCA                                                                             >  SRR4302535.3253565/1‑94 (MQ=60)

                  CCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCA                                                                             <  SRR4302535.4135115/94‑1 (MQ=60)

                   CAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAG                                                                            >  SRR4302535.1316304/1‑94 (MQ=60)

                   CAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAG                                                                            <  SRR4302535.1014459/94‑1 (MQ=60)

                   CAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAG                                                                            >  SRR4302535.4625168/1‑94 (MQ=60)

                    AATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGT                                                                           >  SRR4302535.2894705/1‑94 (MQ=60)

                    AATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGT                                                                           <  SRR4302535.3854872/94‑1 (MQ=60)

                      TCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTT                                                                         >  SRR4302535.1308660/1‑94 (MQ=60)

                      TCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTT                                                                         >  SRR4302535.1166854/1‑94 (MQ=60)

                      TCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTT                                                                         >  SRR4302535.1587564/1‑94 (MQ=60)

                      TCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTT                                                                         <  SRR4302535.2539455/94‑1 (MQ=60)

                      TCACATATTTAATGCCCTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTT                                                                         <  SRR4302535.387676/94‑1 (MQ=60)

                       CACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTG                                                                        >  SRR4302535.2034586/1‑94 (MQ=60)

                         CATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTT                                                                      <  SRR4302535.3773566/94‑1 (MQ=60)

                         CATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTT                                                                      <  SRR4302535.1473672/94‑1 (MQ=60)

                          ATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTG                                                                     >  SRR4302535.3850349/1‑94 (MQ=60)

                          ATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTG                                                                     <  SRR4302535.2247474/94‑1 (MQ=60)

                          ATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTG                                                                     >  SRR4302535.4548745/1‑94 (MQ=60)

                          ATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTG                                                                     >  SRR4302535.2093065/1‑94 (MQ=60)

                           TATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCTCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGC                                                                    <  SRR4302535.771629/94‑1 (MQ=60)

                            ATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCC                                                                   >  SRR4302535.3364080/1‑94 (MQ=60)

                            ATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCC                                                                   >  SRR4302535.3472717/1‑94 (MQ=60)

                             TTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCT                                                                  <  SRR4302535.4292792/94‑1 (MQ=60)

                              TTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTT                                                                 >  SRR4302535.2359550/1‑94 (MQ=60)

                               TAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTT                                                                >  SRR4302535.2176582/1‑94 (MQ=60)

                               TAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTT                                                                >  SRR4302535.4462212/1‑94 (MQ=60)

                                AATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTA                                                               >  SRR4302535.3536713/1‑94 (MQ=60)

                                AATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGCTGCCTTTA                                                               >  SRR4302535.2949931/1‑94 (MQ=60)

                                 ATGCCGTCCTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAA                                                              >  SRR4302535.1403984/1‑94 (MQ=60)

                                  TGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAAT                                                             <  SRR4302535.487567/94‑1 (MQ=60)

                                  TGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAAT                                                             <  SRR4302535.2927343/94‑1 (MQ=60)

                                  TGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAAT                                                             <  SRR4302535.2425468/94‑1 (MQ=60)

                                   TCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATG                                                            <  SRR4302535.1272974/94‑1 (MQ=60)

                                   GCCGTCGTTAACGACTTTGTTCGCCACCGCAACCTCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATG                                                            <  SRR4302535.631469/94‑1 (MQ=60)

                                   GCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATG                                                            <  SRR4302535.4574912/94‑1 (MQ=60)

                                   GCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATG                                                            <  SRR4302535.2526334/94‑1 (MQ=60)

                                   GCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATG                                                            <  SRR4302535.2356769/94‑1 (MQ=60)

                                    CCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGC                                                           >  SRR4302535.939573/1‑94 (MQ=60)

                                     CGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCC                                                          <  SRR4302535.70323/94‑1 (MQ=60)

                                     CGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCC                                                          >  SRR4302535.1599041/1‑94 (MQ=60)

                                     CGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCC                                                          <  SRR4302535.2383923/94‑1 (MQ=60)

                                      GTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCG                                                         <  SRR4302535.4301994/94‑1 (MQ=60)

                                      GTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCG                                                         >  SRR4302535.2852565/1‑94 (MQ=60)

                                         GTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCT                                                      >  SRR4302535.4045358/1‑94 (MQ=60)

                                         GTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCT                                                      >  SRR4302535.2148222/1‑94 (MQ=60)

                                          TTAACGACTTTGTTCGCCACCGCAACCTCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTT                                                     <  SRR4302535.1073347/94‑1 (MQ=60)

                                          TTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTT                                                     <  SRR4302535.3238802/94‑1 (MQ=60)

                                          TTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCAGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTT                                                     <  SRR4302535.4434948/94‑1 (MQ=60)

                                           TAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTT                                                    >  SRR4302535.2840586/1‑94 (MQ=60)

                                               tcaaatcgtaaaatatggcgatGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCG                                                <  SRR4302535.3635652/72‑1 (MQ=60)

                                               TACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCG                                                <  SRR4302535.3778228/94‑1 (MQ=60)

                                               GACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCG                                                >  SRR4302535.4598542/1‑94 (MQ=60)

                                                 CTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTT                                              <  SRR4302535.725906/94‑1 (MQ=60)

                                                 CTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTT                                              >  SRR4302535.1614048/1‑94 (MQ=60)

                                                   TTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGA                                            <  SRR4302535.1539428/94‑1 (MQ=60)

                                                   TTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGA                                            >  SRR4302535.1694369/1‑94 (MQ=60)

                                                   TTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGA                                            <  SRR4302535.3841594/94‑1 (MQ=60)

                                                    TGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGAT                                           <  SRR4302535.1677368/94‑1 (MQ=60)

                                                    TGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGAT                                           <  SRR4302535.4135062/94‑1 (MQ=60)

                                                     GTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATA                                          <  SRR4302535.1484033/94‑1 (MQ=60)

                                                      TTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATAT                                         <  SRR4302535.4420952/94‑1 (MQ=60)

                                                      TTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATAT                                         <  SRR4302535.106192/94‑1 (MQ=60)

                                                      TTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATAT                                         <  SRR4302535.3701970/94‑1 (MQ=60)

                                                      TTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATAT                                         <  SRR4302535.4045669/94‑1 (MQ=60)

                                                         GCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCG                                      >  SRR4302535.4356437/1‑94 (MQ=60)

                                                         GCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCG                                      <  SRR4302535.3796379/94‑1 (MQ=60)

                                                          CCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGC                                     >  SRR4302535.993892/1‑94 (MQ=60)

                                                          CCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGC                                     >  SRR4302535.1679001/1‑94 (MQ=60)

                                                           CACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCA                                    >  SRR4302535.1849470/1‑94 (MQ=60)

                                                           CACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCA                                    >  SRR4302535.2882826/1‑94 (MQ=60)

                                                           CACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCA                                    >  SRR4302535.2965723/1‑94 (MQ=60)

                                                           CACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCA                                    >  SRR4302535.3125043/1‑94 (MQ=60)

                                                            CCCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAA                                   <  SRR4302535.3844139/94‑1 (MQ=60)

                                                            ACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAC                                   >  SRR4302535.2410850/1‑94 (MQ=60)

                                                            ACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAA                                   >  SRR4302535.4332495/1‑94 (MQ=60)

                                                            ACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAA                                   >  SRR4302535.4159053/1‑94 (MQ=60)

                                                            ACCGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAA                                   >  SRR4302535.2278452/1‑94 (MQ=60)

                                                              CGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACC                                 >  SRR4302535.1391099/1‑94 (MQ=60)

                                                              CGCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACC                                 <  SRR4302535.3205440/94‑1 (MQ=60)

                                                               GCAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCG                                >  SRR4302535.1313056/1‑94 (MQ=60)

                                                                CAACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGC                               >  SRR4302535.3162852/1‑94 (MQ=60)

                                                                  ACCGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCT                             <  SRR4302535.3417495/94‑1 (MQ=60)

                                                                   CCTCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTG                            <  SRR4302535.2669346/94‑1 (MQ=60)

                                                                    CGCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGC                           >  SRR4302535.3277023/1‑94 (MQ=60)

                                                                     GCCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCT                          <  SRR4302535.1030238/94‑1 (MQ=60)

                                                                      CCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTC                         >  SRR4302535.4203402/1‑94 (MQ=60)

                                                                      CCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTC                         >  SRR4302535.2023507/1‑94 (MQ=60)

                                                                      CCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTC                         >  SRR4302535.1690402/1‑94 (MQ=60)

                                                                      CCTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTC                         >  SRR4302535.1738160/1‑94 (MQ=60)

                                                                       CTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCT                        <  SRR4302535.1309370/94‑1 (MQ=60)

                                                                       CTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCT                        >  SRR4302535.463213/1‑94 (MQ=60)

                                                                       CTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCT                        <  SRR4302535.1566371/94‑1 (MQ=60)

                                                                       CTGTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCT                        >  SRR4302535.2857620/1‑94 (MQ=60)

                                                                        TGTTTCGGGTCACAGTCATCGCCATATTTGACGAGTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTG                       <  SRR4302535.2769607/94‑1 (MQ=60)

                                                                         GTTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGC                      >  SRR4302535.4507137/1‑94 (MQ=60)

                                                                          TTTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCG                     >  SRR4302535.603388/1‑94 (MQ=60)

                                                                           TTCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGC                    >  SRR4302535.679779/1‑94 (MQ=60)

                                                                            TCGGGTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCC                   >  SRR4302535.1149153/1‑94 (MQ=60)

                                                                                GTCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTA               >  SRR4302535.372363/1‑94 (MQ=60)

                                                                                 TCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAA              <  SRR4302535.3419893/94‑1 (MQ=60)

                                                                                 TCACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAA              >  SRR4302535.1159117/1‑94 (MQ=60)

                                                                                  CACAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAA             >  SRR4302535.1662301/1‑94 (MQ=60)

                                                                                    CAGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACT           >  SRR4302535.4578881/1‑94 (MQ=60)

                                                                                     AGGCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTC          >  SRR4302535.3964129/1‑94 (MQ=60)

                                                                                       GCATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCT        <  SRR4302535.4318791/94‑1 (MQ=60)

                                                                                        CATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTG       >  SRR4302535.1308711/1‑94 (MQ=60)

                                                                                        CATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTG       <  SRR4302535.2187685/94‑1 (MQ=60)

                                                                                        CATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTG       <  SRR4302535.1663726/94‑1 (MQ=60)

                                                                                         ATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACTGCCTGCTCTGCGACGGTAAACTCCTGG      >  SRR4302535.1462526/1‑94 (MQ=60)

                                                                                         ATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGG      >  SRR4302535.3771737/1‑94 (MQ=60)

                                                                                         ATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGG      >  SRR4302535.378725/1‑94 (MQ=60)

                                                                                         ATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGG      >  SRR4302535.295629/1‑94 (MQ=60)

                                                                                         ATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGG      >  SRR4302535.1672317/1‑94 (MQ=60)

                                                                                         ATCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGG      <  SRR4302535.434032/94‑1 (MQ=60)

                                                                                          TCGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGGT     <  SRR4302535.4354424/94‑1 (MQ=60)

                                                                                           CGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGGTC    >  SRR4302535.2152599/1‑94 (MQ=60)

                                                                                           CGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGGTC    <  SRR4302535.2857265/94‑1 (MQ=60)

                                                                                           CGCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGGTC    >  SRR4302535.1871877/1‑94 (MQ=60)

                                                                                            GCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGGTCA   >  SRR4302535.2036864/1‑94 (MQ=60)

                                                                                            GCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGGTCA   >  SRR4302535.2897042/1‑94 (MQ=60)

                                                                                            GCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGGTCA   <  SRR4302535.1376694/94‑1 (MQ=60)

                                                                                            GCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGGTCA   <  SRR4302535.57343/94‑1 (MQ=60)

                                                                                            GCCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGGTCA   <  SRR4302535.2462454/94‑1 (MQ=60)

                                                                                             CCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGGTCAC  >  SRR4302535.4045308/1‑94 (MQ=60)

                                                                                             CCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGGTCAC  >  SRR4302535.117388/1‑94 (MQ=60)

                                                                                             CCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGGTCAC  >  SRR4302535.2680110/1‑94 (MQ=60)

GATGAAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGGTCAC  >  NC_000913/3596979‑3597165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`