Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,623,435 T→C I334V (ATC→GTC)  b3485 ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,623,4350TC100.0% 665.4 / NA 213I334V (ATC→GTC) b3485putative transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (94/119);  total (94/119)

Only 100 of 219 total aligned reads displayed.

GCAGCGCAATGGTGAAAAATGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGATGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCGCGGCGTGGAAC  >  NC_000913/3623345‑3623524
                                                                                          |                                                                                         
gcagcGCAATGGTGAAAAATGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACgg                                                                                         >  1:2235151/1‑93 (MQ=255)
 cagcGCAATGGTGAAAAATGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGc                                                                                        <  1:4188485/93‑1 (MQ=255)
  agcGCAATGGTGAAAAATGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGcc                                                                                       >  1:3210130/1‑93 (MQ=255)
    cgcAATGGTGAAAAATGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTg                                                                                     >  2:66411/1‑93 (MQ=255)
     gcAATGGTGAAAAATGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTgc                                                                                    >  2:1306457/1‑93 (MQ=255)
     gcAATGGTGAAAAATGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTgc                                                                                    >  1:919650/1‑93 (MQ=255)
     gcAATGGTGAAAAATGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTgc                                                                                    <  1:4325250/93‑1 (MQ=255)
      cAATGGTGAAAAATGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTgcg                                                                                   >  1:1426884/1‑93 (MQ=255)
         tGGTGAAAAATGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGa                                                                                >  1:1516886/1‑93 (MQ=255)
          ggTGAAAAATGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAg                                                                               >  1:2978613/1‑93 (MQ=255)
          ggTGAAAAATGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAg                                                                               <  2:4173010/93‑1 (MQ=255)
           gTGAAAAATGCGCCGCCAATTGACATCAGCGTCAGAAACTGCGGCCAGACGCTTTCGAATCCGGCACCCCGGTAGAGGACGGACTGCGCGAgg                                                                              >  2:929023/1‑93 (MQ=255)
            tGAAAAATGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGc                                                                             >  1:1704179/1‑93 (MQ=255)
               aaaaTGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTaa                                                                          <  2:3431682/93‑1 (MQ=255)
                   tGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACaaa                                                                      >  1:831961/1‑93 (MQ=255)
                   tGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACaaa                                                                      <  1:3958007/93‑1 (MQ=255)
                   tGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACaaa                                                                      <  2:2209855/93‑1 (MQ=255)
                    gcgcCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAg                                                                     <  1:2403727/93‑1 (MQ=255)
                      gccgccAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAgtg                                                                   <  2:1919333/93‑1 (MQ=255)
                       ccgccAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAgtgt                                                                  <  2:4469477/93‑1 (MQ=255)
                        cgccAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAgtgtg                                                                 <  1:4622609/93‑1 (MQ=255)
                        cgccAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAgtgtg                                                                 <  2:4161678/93‑1 (MQ=255)
                          ccAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTgtc                                                               <  2:4405224/93‑1 (MQ=255)
                           cAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTgtcg                                                              >  1:3660207/1‑93 (MQ=255)
                            aaTTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTgtcgt                                                             >  1:3141916/1‑93 (MQ=255)
                             aTTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTgtcgtc                                                            >  2:2762057/1‑93 (MQ=255)
                             aTTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTgtcgtc                                                            <  2:2951003/93‑1 (MQ=255)
                              ttGCCATCAGGGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGtcgtcg                                                           <  1:1260639/93‑1 (MQ=255)
                              ttGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGtcgtcg                                                           >  1:885695/1‑93 (MQ=255)
                              ttGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGtcgtcg                                                           >  1:196541/1‑93 (MQ=255)
                                gCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGCGTGGTCGGc                                                         >  2:3631800/1‑93 (MQ=255)
                                  cATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCAt                                                       >  2:227824/1‑93 (MQ=255)
                                   aTCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATg                                                      >  1:3225972/1‑93 (MQ=255)
                                   aTCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATg                                                      >  2:2543938/1‑93 (MQ=255)
                                   aTCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATg                                                      >  2:1069419/1‑93 (MQ=255)
                                    tCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATgg                                                     >  1:3524962/1‑93 (MQ=255)
                                    tCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATgg                                                     >  2:1602153/1‑93 (MQ=255)
                                    tCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATgg                                                     <  1:3377849/93‑1 (MQ=255)
                                     cAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGt                                                    >  2:2858664/1‑93 (MQ=255)
                                       gCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCa                                                  <  1:3765066/93‑1 (MQ=255)
                                       gCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCa                                                  <  1:2733535/93‑1 (MQ=255)
                                          tCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCa                                               >  1:1607304/1‑93 (MQ=255)
                                          tCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCa                                               <  1:3831227/93‑1 (MQ=255)
                                            aGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATa                                             >  2:424980/1‑93 (MQ=255)
                                            aGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATa                                             <  2:1571300/93‑1 (MQ=255)
                                             gAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATaa                                            >  1:2858649/1‑93 (MQ=255)
                                              aaaCTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAAt                                           <  2:4630945/93‑1 (MQ=255)
                                              aaaCTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAAt                                           <  2:2624221/93‑1 (MQ=255)
                                               aaCTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATg                                          <  1:2618016/93‑1 (MQ=255)
                                               aaCTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATg                                          <  2:1556000/93‑1 (MQ=255)
                                               aaCTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATg                                          <  2:3707442/93‑1 (MQ=255)
                                                aCTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGt                                         >  2:44337/1‑93 (MQ=255)
                                                aCTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGt                                         <  2:755565/93‑1 (MQ=255)
                                                 cTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTc                                        <  2:1143354/93‑1 (MQ=255)
                                                  tGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTcc                                       <  2:3553089/93‑1 (MQ=255)
                                                  tGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTcc                                       <  2:4026840/93‑1 (MQ=255)
                                                     ggCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGc                                    <  1:2265043/93‑1 (MQ=255)
                                                      gCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCa                                   >  1:2818484/1‑93 (MQ=255)
                                                       ccAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCAc                                  >  2:2317957/1‑93 (MQ=255)
                                                        cAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCAcc                                 >  2:3322038/1‑93 (MQ=255)
                                                        cAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCAcc                                 >  1:1968355/1‑93 (MQ=255)
                                                        cAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCAcc                                 <  2:2466031/93‑1 (MQ=255)
                                                         aGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCa                                <  1:2169746/93‑1 (MQ=255)
                                                         aGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCa                                <  2:520249/93‑1 (MQ=255)
                                                         aGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCa                                <  1:1192779/93‑1 (MQ=255)
                                                          gACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCAt                               >  1:1066935/1‑93 (MQ=255)
                                                          gACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCAt                               >  1:2113916/1‑93 (MQ=255)
                                                          gACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCAt                               >  2:3100279/1‑93 (MQ=255)
                                                           aCGATTCCGAATCCGGCACCCCGGTAGAGGACGGCCGGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATc                              <  2:2390115/93‑1 (MQ=255)
                                                            cGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCt                             <  1:2076598/93‑1 (MQ=255)
                                                              aTTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGc                           >  2:323892/1‑93 (MQ=255)
                                                                 tCGAATCCGGCACCCCGGTAGAGGACTGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGc                        <  1:1802982/93‑1 (MQ=255)
                                                                   gAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCAt                      >  1:811628/1‑93 (MQ=255)
                                                                    aaTCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATa                     <  2:134913/93‑1 (MQ=255)
                                                                     aTCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATAc                    <  1:448963/93‑1 (MQ=255)
                                                                      tCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACt                   <  1:3579492/93‑1 (MQ=255)
                                                                      tCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACt                   >  2:3907971/1‑93 (MQ=255)
                                                                      tCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACt                   >  2:4371950/1‑93 (MQ=255)
                                                                        cGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGATCAGCATAATGTCCTGCACCATCTGCGGCATACttt                 <  1:3564291/93‑1 (MQ=255)
                                                                         ggCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTc                >  2:1253288/1‑93 (MQ=255)
                                                                          gCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTcg               <  2:2241914/93‑1 (MQ=255)
                                                                           cACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTcgc              <  1:552715/93‑1 (MQ=255)
                                                                            aCCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTcgcg             <  2:814051/93‑1 (MQ=255)
                                                                            aCCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTcgcg             >  1:2225406/1‑93 (MQ=255)
                                                                              cccGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTcgcgcg           <  1:3840887/93‑1 (MQ=255)
                                                                              cccGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTcgcgcg           >  2:3033737/1‑93 (MQ=255)
                                                                              cccGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTcgcgcg           >  2:1726354/1‑93 (MQ=255)
                                                                               ccGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCgcgg          >  1:4444944/1‑93 (MQ=255)
                                                                               ccGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCgcgg          <  2:774185/93‑1 (MQ=255)
                                                                                cGGTAGAGGGCGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCgcggc         <  2:306971/93‑1 (MQ=255)
                                                                                cGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCgcggc         >  1:3040620/1‑93 (MQ=255)
                                                                                  gTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCGCGGCGt       <  1:1352200/93‑1 (MQ=255)
                                                                                  gTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCGCGGCGt       <  1:2382816/93‑1 (MQ=255)
                                                                                  gTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCGCGGCGt       >  1:4023432/1‑93 (MQ=255)
                                                                                  gTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCGCGGCGt       <  1:2307143/93‑1 (MQ=255)
                                                                                  gTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCGCGGCGt       >  2:2425114/1‑93 (MQ=255)
                                                                                    agagGACGGCCTGCGCGAGGCTAACAAATTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCGCGGCGTgg     <  1:3067547/93‑1 (MQ=255)
                                                                                     gagGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCGCGGCGTGGa    >  1:4067769/1‑93 (MQ=255)
                                                                                       ggACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCGCGGCGTGGAac  >  1:2359980/1‑93 (MQ=255)
                                                                                       ggACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCGCGGCGTGGAac  <  2:1829497/93‑1 (MQ=255)
                                                                                          |                                                                                         
GCAGCGCAATGGTGAAAAATGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGATGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCGCGGCGTGGAAC  >  NC_000913/3623345‑3623524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCAGCAGCGCAATGGTGAAAAATGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGATGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCGCGGCGTGGAACCACC  >  NC_000913/3623342‑3623528
                                                                                             |                                                                                             
GCAGCAGCGCAATGGTGAAAAATGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGAC                                                                                               <  SRR4302535.2354948/94‑1 (MQ=60)
 CAGCAGCGCAATGGTGAAAAATGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACG                                                                                              <  SRR4302535.2076934/94‑1 (MQ=60)
 CAGCAGCGCAATGGTGAAAAATGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACG                                                                                              <  SRR4302535.3744468/94‑1 (MQ=60)
  AGCAGCGCAATGGTGAAAAATGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGG                                                                                             >  SRR4302535.2251052/1‑94 (MQ=60)
  AGCAGCGCAATGGTGAAAAATGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGG                                                                                             <  SRR4302535.2733710/94‑1 (MQ=60)
  AGCAGCGCAATGGTGAAAAATGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGG                                                                                             >  SRR4302535.388115/1‑94 (MQ=60)
  AGCAGCGCAATGGTGAAAAATGCGCCGCCAAGTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTGCAAATCCGGCACCCCGGTAGAGGACGG                                                                                             >  SRR4302535.280982/1‑94 (MQ=60)
    CAGCGCAATGGTGAAAAATGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCC                                                                                           >  SRR4302535.3232366/1‑94 (MQ=60)
    CAGCGCAATGGTGAAAAATGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCC                                                                                           <  SRR4302535.4217155/94‑1 (MQ=60)
    CAGCGCAATGGTGAAAAATGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCC                                                                                           <  SRR4302535.2895465/94‑1 (MQ=60)
     AGCGCAATGGTGAAAAATGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCT                                                                                          >  SRR4302535.1656352/1‑94 (MQ=60)
       CGCAATGGTGAAAAATGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGC                                                                                        >  SRR4302535.926172/1‑94 (MQ=60)
       CGCAATGGTGAAAAATGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGC                                                                                        >  SRR4302535.2256526/1‑94 (MQ=60)
        GCAATGGTGAAAAATGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGTCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCG                                                                                       <  SRR4302535.4215605/94‑1 (MQ=60)
        GCAATGGTGAAAAATGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCG                                                                                       <  SRR4302535.3603386/94‑1 (MQ=60)
        GCAATGGTGAAAAATGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCG                                                                                       >  SRR4302535.1437070/1‑94 (MQ=60)
        GCAATGGTGAAAAATGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCG                                                                                       <  SRR4302535.4354829/94‑1 (MQ=60)
         CAATGGTGAAAAATGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGC                                                                                      >  SRR4302535.2680647/1‑94 (MQ=60)
           ATGGTGAAAAATGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGA                                                                                    >  SRR4302535.211694/1‑94 (MQ=60)
           ATGGTGAAAAATGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGA                                                                                    >  SRR4302535.1527813/1‑94 (MQ=60)
            TGGTGAAAAATGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAG                                                                                   >  SRR4302535.2999512/1‑94 (MQ=60)
            TGGTGAAAAATGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAG                                                                                   >  SRR4302535.2026483/1‑94 (MQ=60)
              GTGAAAAATGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGC                                                                                 >  SRR4302535.1181904/1‑94 (MQ=60)
              GTGAAAAATGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGC                                                                                 >  SRR4302535.1716499/1‑94 (MQ=60)
               TGAAAAATGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCT                                                                                >  SRR4302535.3986693/1‑94 (MQ=60)
                  AAAATGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAAC                                                                             >  SRR4302535.3240022/1‑94 (MQ=60)
                   AAATGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGCCCTGCGCGcggcacaca                                                                            >  SRR4302535.1304089/1‑85 (MQ=60)
                     ATGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAA                                                                          <  SRR4302535.1537389/94‑1 (MQ=60)
                     ATGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAA                                                                          >  SRR4302535.837791/1‑94 (MQ=60)
                      TGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAG                                                                         <  SRR4302535.33604/94‑1 (MQ=60)
                      TGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAG                                                                         <  SRR4302535.3985035/94‑1 (MQ=60)
                       GCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGT                                                                        <  SRR4302535.2421019/94‑1 (MQ=60)
                        CGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTG                                                                       <  SRR4302535.3844051/94‑1 (MQ=60)
                          CCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTG                                                                     <  SRR4302535.4274634/94‑1 (MQ=60)
                          CCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTG                                                                     >  SRR4302535.4526716/1‑94 (MQ=60)
                           CGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGT                                                                    >  SRR4302535.4494116/1‑94 (MQ=60)
                           CGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGT                                                                    <  SRR4302535.4654007/94‑1 (MQ=60)
                            CCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTC                                                                   <  SRR4302535.868507/94‑1 (MQ=60)
                             CCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCG                                                                  <  SRR4302535.1230056/94‑1 (MQ=60)
                             CCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCG                                                                  >  SRR4302535.3685543/1‑94 (MQ=60)
                              CAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGT                                                                 >  SRR4302535.3163777/1‑94 (MQ=60)
                                ATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCG                                                               >  SRR4302535.891962/1‑94 (MQ=60)
                                ATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCG                                                               >  SRR4302535.197893/1‑94 (MQ=60)
                                 TTGCCATCAGGGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGG                                                              <  SRR4302535.1269660/94‑1 (MQ=60)
                                 TTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGG                                                              <  SRR4302535.4398335/94‑1 (MQ=60)
                                   GCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCA                                                            <  SRR4302535.3566286/94‑1 (MQ=60)
                                    CCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCAT                                                           <  SRR4302535.3088107/94‑1 (MQ=60)
                                    CCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCAT                                                           >  SRR4302535.279025/1‑94 (MQ=60)
                                     CATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATG                                                          >  SRR4302535.3248337/1‑94 (MQ=60)
                                     CATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATG                                                          <  SRR4302535.3783679/94‑1 (MQ=60)
                                      ATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGG                                                         >  SRR4302535.3549440/1‑94 (MQ=60)
                                      ATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGG                                                         >  SRR4302535.1775871/1‑94 (MQ=60)
                                       TCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGT                                                        <  SRR4302535.3401336/94‑1 (MQ=60)
                                        CAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTC                                                       <  SRR4302535.3045044/94‑1 (MQ=60)
                                        CAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTC                                                       <  SRR4302535.2043028/94‑1 (MQ=60)
                                         AGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCA                                                      >  SRR4302535.1417110/1‑94 (MQ=60)
                                          GCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAG                                                     <  SRR4302535.3791071/94‑1 (MQ=60)
                                          GCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAG                                                     <  SRR4302535.2753043/94‑1 (MQ=60)
                                           CGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGC                                                    <  SRR4302535.3640327/94‑1 (MQ=60)
                                           CGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGC                                                    <  SRR4302535.2386909/94‑1 (MQ=60)
                                            GTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCA                                                   >  SRR4302535.1618900/1‑94 (MQ=60)
                                            GTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCA                                                   <  SRR4302535.2651665/94‑1 (MQ=60)
                                             TCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCAT                                                  <  SRR4302535.3857647/94‑1 (MQ=60)
                                              CAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATA                                                 <  SRR4302535.2401247/94‑1 (MQ=60)
                                               AGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAA                                                >  SRR4302535.2878901/1‑94 (MQ=60)
                                                 AAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATG                                              <  SRR4302535.3232057/94‑1 (MQ=60)
                                                  AACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGT                                             <  SRR4302535.2636785/94‑1 (MQ=60)
                                                  AACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGT                                             >  SRR4302535.2243441/1‑94 (MQ=60)
                                                     TGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCT                                          >  SRR4302535.4064989/1‑94 (MQ=60)
                                                        GGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCA                                       <  SRR4302535.385131/94‑1 (MQ=60)
                                                        GGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCA                                       <  SRR4302535.2281184/94‑1 (MQ=60)
                                                        GGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCA                                       >  SRR4302535.2838504/1‑94 (MQ=60)
                                                          CCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACC                                     >  SRR4302535.1982540/1‑94 (MQ=60)
                                                          CCAGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACC                                     >  SRR4302535.2855940/1‑94 (MQ=60)
                                                            AGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCAT                                   >  SRR4302535.1074523/1‑94 (MQ=60)
                                                            AGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCAT                                   <  SRR4302535.1201327/94‑1 (MQ=60)
                                                            AGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCAT                                   <  SRR4302535.2185285/94‑1 (MQ=60)
                                                            AGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCAT                                   >  SRR4302535.2129172/1‑94 (MQ=60)
                                                            AGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCAT                                   <  SRR4302535.659129/94‑1 (MQ=60)
                                                            AGACGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCAT                                   <  SRR4302535.1939911/94‑1 (MQ=60)
                                                               CGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTG                                <  SRR4302535.4331815/94‑1 (MQ=60)
                                                               CGATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTG                                <  SRR4302535.2091558/94‑1 (MQ=60)
                                                                 ATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCG                              <  SRR4302535.830219/94‑1 (MQ=60)
                                                                 ATTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCG                              >  SRR4302535.1296860/1‑94 (MQ=60)
                                                                  TTTCGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGG                             <  SRR4302535.2277854/94‑1 (MQ=60)
                                                                    TCGAATCCGGCACCCCGGTAGAGGACTGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCA                           <  SRR4302535.1816014/94‑1 (MQ=60)
                                                                     CGAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCAT                          >  SRR4302535.817304/1‑94 (MQ=60)
                                                                      GAATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATA                         >  SRR4302535.198126/1‑94 (MQ=60)
                                                                       AATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATAC                        <  SRR4302535.569820/94‑1 (MQ=60)
                                                                        ATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACT                       <  SRR4302535.452108/94‑1 (MQ=60)
                                                                        ATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACT                       >  SRR4302535.2311422/1‑94 (MQ=60)
                                                                        ATCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACT                       >  SRR4302535.3503000/1‑94 (MQ=60)
                                                                         TCCGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTT                      <  SRR4302535.3604303/94‑1 (MQ=60)
                                                                           CGGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGATCAGCATAATGTCCTGCACCATCTGCGGCATACTTTC                    <  SRR4302535.3589003/94‑1 (MQ=60)
                                                                            GGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCG                   <  SRR4302535.2375452/94‑1 (MQ=60)
                                                                            GGCACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCG                   <  SRR4302535.2137422/94‑1 (MQ=60)
                                                                              CACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCG                 <  SRR4302535.556586/94‑1 (MQ=60)
                                                                              CACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCG                 >  SRR4302535.2241241/1‑94 (MQ=60)
                                                                              CACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCG                 <  SRR4302535.2202240/94‑1 (MQ=60)
                                                                              CACCCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCG                 <  SRR4302535.1444872/94‑1 (MQ=60)
                                                                                 CCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCGCGG              <  SRR4302535.3867353/94‑1 (MQ=60)
                                                                                 CCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCGCGG              <  SRR4302535.3867352/94‑1 (MQ=60)
                                                                                 CCCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCGCGG              >  SRR4302535.4475425/1‑94 (MQ=60)
                                                                                  CCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCGCGGC             >  SRR4302535.3527072/1‑94 (MQ=60)
                                                                                  CCGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCGCGGC             >  SRR4302535.3061920/1‑94 (MQ=60)
                                                                                   CGGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCGCGGCG            <  SRR4302535.1567681/94‑1 (MQ=60)
                                                                                    GGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCGCGGCGT           >  SRR4302535.3121283/1‑94 (MQ=60)
                                                                                    GGTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCGCGGCGT           >  SRR4302535.4050958/1‑94 (MQ=60)
                                                                                     GTAGAGGACGGCGTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATGCTTTCGCGCGGCGTG          <  SRR4302535.922371/94‑1 (MQ=60)
                                                                                     GTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCGCGGCGTG          <  SRR4302535.2399928/94‑1 (MQ=60)
                                                                                     GTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCGCGGCGTG          <  SRR4302535.1361878/94‑1 (MQ=60)
                                                                                     GTAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCGCGGCGTG          <  SRR4302535.2323646/94‑1 (MQ=60)
                                                                                      TAGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCGCGGCGTGG         <  SRR4302535.2505211/94‑1 (MQ=60)
                                                                                       AGAGGACGGCCTGCGCGAGGCTAACAAATTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCGCGGCGTGGA        <  SRR4302535.3088978/94‑1 (MQ=60)
                                                                                       AGAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCGCGGCGTGGA        >  SRR4302535.4095603/1‑94 (MQ=60)
                                                                                        GAGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCGCGGCGTGGAA       <  SRR4302535.489667/94‑1 (MQ=60)
                                                                                         AGGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCGCGGCGTGGAAC      >  SRR4302535.2376916/1‑94 (MQ=60)
                                                                                          GGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCGCGGCGTGGAACC     >  SRR4302535.4006773/1‑94 (MQ=60)
                                                                                          GGACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCGCGGCGTGGAACC     <  SRR4302535.3080057/94‑1 (MQ=60)
                                                                                           GACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCGCGGCGTGGAACCA    <  SRR4302535.3993298/94‑1 (MQ=60)
                                                                                           GACGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCGCGGCGTGGAACCA    >  SRR4302535.2149408/1‑94 (MQ=60)
                                                                                             CGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCGCGGCGTGGAACCACC  <  SRR4302535.4120626/94‑1 (MQ=60)
                                                                                             CGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCGCGGCGTGGAACCACC  <  SRR4302535.825892/94‑1 (MQ=60)
                                                                                             |                                                                                             
GCAGCAGCGCAATGGTGAAAAATGCGCCGCCAATTGCCATCAGCGTCAGAAACTGCGGCCAGACGATTTCGAATCCGGCACCCCGGTAGAGGATGGCCTGCGCGAGGCTAACAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCGCGGCGTGGAACCACC  >  NC_000913/3623342‑3623528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑