Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,634,228	A→G	R271R (CGT→CGC)	yhiN ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,634,228	0	A	G	99.5%	641.6 / NA	207	R271R (CGT→CGC)	yhiN	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (111/95); total (112/95)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 219 total aligned reads displayed.

GATTTGCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTACGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGC  >  NC_000913/3634138‑3634318

 aTTTGCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTgcgc                                                                                         >  1:3317208/1‑93 (MQ=255)

 aTTTGCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTgcgc                                                                                         <  2:2282855/93‑1 (MQ=255)

 aTTTGCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTgcgc                                                                                         >  2:4234459/1‑93 (MQ=255)

 aTTTGCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTgcgc                                                                                         <  1:1884275/93‑1 (MQ=255)

 aTTTGCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTgcgc                                                                                         <  1:4606313/93‑1 (MQ=255)

  tttGCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCgct                                                                                        >  2:1344195/1‑93 (MQ=255)

   ttGCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCgctg                                                                                       <  1:2654567/93‑1 (MQ=255)

    tGCCCGAGTTGCTGTAAGCGTTCACCCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCgctgc                                                                                      <  1:2540161/93‑1 (MQ=255)

       ccGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCa                                                                                   >  2:575042/1‑93 (MQ=255)

        cGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCAt                                                                                  >  2:4024088/1‑93 (MQ=255)

        cGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCAt                                                                                  >  1:1910358/1‑93 (MQ=255)

         gAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGGGCTGCTCAtt                                                                                 >  2:2462240/1‑93 (MQ=255)

         gAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCAtt                                                                                 >  1:1706143/1‑93 (MQ=255)

         gAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCAtt                                                                                 <  1:1760741/93‑1 (MQ=255)

          aGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCACCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTc                                                                                >  1:3302330/1‑93 (MQ=255)

           gTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCa                                                                               <  1:3363299/93‑1 (MQ=255)

            ttGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAg                                                                              <  2:3947969/93‑1 (MQ=255)

            ttGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAg                                                                              <  2:3401285/93‑1 (MQ=255)

            ttGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAg                                                                              >  1:1476888/1‑93 (MQ=255)

              gCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGa                                                                            >  2:2960433/1‑93 (MQ=255)

                tGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAg                                                                          >  1:1958812/1‑93 (MQ=255)

                tGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAg                                                                          <  1:3741362/93‑1 (MQ=255)

                tGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAg                                                                          >  2:304650/1‑93 (MQ=255)

                tGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAg                                                                          >  2:3763816/1‑93 (MQ=255)

                tGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAg                                                                          <  2:4158350/93‑1 (MQ=255)

                 gTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAgg                                                                         >  2:1151328/1‑93 (MQ=255)

                  tAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGt                                                                        >  1:2474149/1‑93 (MQ=255)

                  tAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGt                                                                        <  2:2204297/93‑1 (MQ=255)

                  tAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGt                                                                        <  1:4568947/93‑1 (MQ=255)

                  tAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGt                                                                        <  1:3616459/93‑1 (MQ=255)

                     gctttCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTc                                                                     <  1:1132160/90‑1 (MQ=255)

                     gCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTc                                                                     >  1:4668890/1‑93 (MQ=255)

                     gCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTc                                                                     <  1:704938/93‑1 (MQ=255)

                     gCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTc                                                                     <  2:2810174/93‑1 (MQ=255)

                     gCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTc                                                                     >  2:642258/1‑93 (MQ=255)

                      cGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCg                                                                    >  1:3036594/1‑93 (MQ=255)

                       gTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGa                                                                   <  1:2713491/93‑1 (MQ=255)

                       gTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGa                                                                   <  1:2300828/93‑1 (MQ=255)

                          caaccaacCGCTTCGGTAGATGAACCGCCAGTGTGCTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGt                                                                >  2:3360357/1‑93 (MQ=255)

                           aaccaaccGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTc                                                               <  2:2879168/93‑1 (MQ=255)

                            accaaccGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTcc                                                              >  1:1682200/1‑93 (MQ=255)

                             ccaaccGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCca                                                             >  1:3518505/1‑93 (MQ=255)

                             ccaaccGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCca                                                             >  1:2091713/1‑93 (MQ=255)

                              caaccGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCcac                                                            >  2:2358564/1‑93 (MQ=255)

                               aaccGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTTGAGGTCcaca                                                           >  1:1288995/1‑93 (MQ=255)

                               aaccGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCcaca                                                           <  2:3310170/93‑1 (MQ=255)

                                accGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACAt                                                          >  1:3208928/1‑93 (MQ=255)

                                 ccGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATc                                                         <  2:302436/93‑1 (MQ=255)

                                 ccGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATc                                                         <  2:3546451/93‑1 (MQ=255)

                                      tCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTa                                                    >  1:4429041/1‑93 (MQ=255)

                                        ggTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGc                                                  >  1:4433941/1‑93 (MQ=255)

                                         gTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCa                                                 <  1:4037862/93‑1 (MQ=255)

                                         gTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCa                                                 >  1:1729948/1‑93 (MQ=255)

                                          tAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTTGAGGTCCACATCCGGGAGCAg                                                >  2:4296413/1‑93 (MQ=255)

                                           aGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGa                                               >  2:3605369/1‑93 (MQ=255)

                                            gATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGAt                                              <  1:601368/93‑1 (MQ=255)

                                            gATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGAt                                              <  1:654382/93‑1 (MQ=255)

                                            gATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGAt                                              >  1:4248458/1‑93 (MQ=255)

                                             aTGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGAtt                                             <  1:395362/93‑1 (MQ=255)

                                               gAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCGGATTGa                                           >  2:455413/1‑93 (MQ=255)

                                               gAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGa                                           >  1:3548625/1‑93 (MQ=255)

                                               gAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGa                                           >  2:1318529/1‑93 (MQ=255)

                                               gAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGa                                           <  1:749148/93‑1 (MQ=255)

                                                 aCCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATg                                         >  1:4311831/1‑93 (MQ=255)

                                                 aCCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATg                                         >  1:3115369/1‑93 (MQ=255)

                                                   cGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCt                                       <  1:140032/93‑1 (MQ=255)

                                                   cGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCt                                       <  1:2613391/93‑1 (MQ=255)

                                                    gCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTg                                      >  2:1078019/1‑93 (MQ=255)

                                                      cAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGAc                                    >  1:882595/1‑93 (MQ=255)

                                                         tgtgtTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACaaa                                 <  1:4341184/93‑1 (MQ=255)

                                                          gtgtTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAAt                                >  2:2010145/1‑93 (MQ=255)

                                                            gtTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCCTTCAGGAAGGCTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTc                              >  2:4392343/1‑93 (MQ=255)

                                                            gtTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTATCGAGGTCCACATCCGGTAGAAGATTAATGCTGACAAATTc                              >  2:1014905/1‑93 (MQ=255)

                                                              ttttCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTccc                            <  2:2401006/93‑1 (MQ=255)

                                                              ttttCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTccc                            <  2:1941933/93‑1 (MQ=255)

                                                              ttttCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTccc                            <  1:3902029/93‑1 (MQ=255)

                                                               tttCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTcccc                           >  2:164259/1‑93 (MQ=255)

                                                                ttCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTccccc                          <  1:931149/93‑1 (MQ=255)

                                                                 tCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCg                         >  1:1587960/1‑93 (MQ=255)

                                                                  cAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCgg                        <  1:3565902/93‑1 (MQ=255)

                                                                   aGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGt                       >  2:3214548/1‑93 (MQ=255)

                                                                   aGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGt                       <  1:812768/93‑1 (MQ=255)

                                                                   aGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGt                       <  2:2533540/93‑1 (MQ=255)

                                                                    ggCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGtt                      <  1:1038489/93‑1 (MQ=255)

                                                                    ggCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGtt                      <  2:1661560/93‑1 (MQ=255)

                                                                      cTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGc                    <  1:341294/93‑1 (MQ=255)

                                                                       tttGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGcc                   >  1:2242821/1‑93 (MQ=255)

                                                                       tttGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGcc                   >  1:1924365/1‑93 (MQ=255)

                                                                       tttGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGCAGCAGATTGATGCTGACAAATTCCCCCGCTTGcc                   >  1:1305031/1‑93 (MQ=255)

                                                                        ttGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCa                  <  1:1866388/93‑1 (MQ=255)

                                                                          gATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGt                >  1:3820245/1‑93 (MQ=255)

                                                                          gATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGt                >  2:486784/1‑93 (MQ=255)

                                                                           aTTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTa               >  2:1753810/1‑93 (MQ=255)

                                                                             tCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGc             >  1:3479336/1‑93 (MQ=255)

                                                                                gATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTg          >  2:2632976/1‑93 (MQ=255)

                                                                                  tgtgCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGaa        >  1:2835405/1‑93 (MQ=255)

                                                                                  tgtgCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGaa        >  2:4291879/1‑93 (MQ=255)

                                                                                   gtgCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTGCCCCGGATGCCCGTAGCTTGaaa       >  2:122769/1‑93 (MQ=255)

                                                                                   gtgCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGaaa       >  1:2075085/1‑93 (MQ=255)

                                                                                    tgCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAAt      <  2:4125316/93‑1 (MQ=255)

GATTTGCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTACGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGC  >  NC_000913/3634138‑3634318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGGGATTTGCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTACGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAAC  >  NC_000913/3634135‑3634321

CGGGATTTGCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTG                                                                                               <  SRR4302535.2735772/94‑1 (MQ=60)

CGGGATTTGCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTG                                                                                               <  SRR4302535.2277476/94‑1 (MQ=60)

CGGGATTTGCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTG                                                                                               <  SRR4302535.2277475/94‑1 (MQ=60)

   GATTTGCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGC                                                                                            >  SRR4302535.3340289/1‑94 (MQ=60)

   GATTTGCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGC                                                                                            <  SRR4302535.1282936/94‑1 (MQ=60)

    ATTTGCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCT                                                                                           <  SRR4302535.4637619/94‑1 (MQ=60)

    ATTTGCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCT                                                                                           <  SRR4302535.1897856/94‑1 (MQ=60)

     TTTGCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTG                                                                                          <  SRR4302535.1005208/94‑1 (MQ=60)

      TTGCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGC                                                                                         <  SRR4302535.2673586/94‑1 (MQ=60)

       TGCCGGAGTTGCTGTAAGCGTCGACCCAACCGCTTCGGTAGATGACCCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCT                                                                                        <  SRR4302535.1837667/94‑1 (MQ=60)

       TGCCCGAGTTGCTGTAAGCGTTCACCCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCT                                                                                        <  SRR4302535.2558456/94‑1 (MQ=60)

        GCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTC                                                                                       <  SRR4302535.3630201/94‑1 (MQ=60)

         CCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCA                                                                                      >  SRR4302535.1908666/1‑94 (MQ=60)

          CCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCAT                                                                                     >  SRR4302535.1924126/1‑94 (MQ=60)

           CGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATT                                                                                    >  SRR4302535.779448/1‑94 (MQ=60)

           CGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATT                                                                                    >  SRR4302535.1718477/1‑94 (MQ=60)

            GAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTC                                                                                   <  SRR4302535.1773490/94‑1 (MQ=60)

            GAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCACCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTC                                                                                   >  SRR4302535.3325294/1‑94 (MQ=60)

             AGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCA                                                                                  <  SRR4302535.355274/94‑1 (MQ=60)

              GTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAG                                                                                 <  SRR4302535.1750860/94‑1 (MQ=60)

              GTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAG                                                                                 >  SRR4302535.1487463/1‑94 (MQ=60)

              GTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAG                                                                                 >  SRR4302535.173304/1‑94 (MQ=60)

              GTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAG                                                                                 <  SRR4302535.1694430/94‑1 (MQ=60)

              GTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAG                                                                                 <  SRR4302535.3386701/94‑1 (MQ=60)

                TGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGA                                                                               <  SRR4302535.4608974/94‑1 (MQ=60)

                  CTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAG                                                                             <  SRR4302535.2545575/94‑1 (MQ=60)

                  CTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAG                                                                             >  SRR4302535.3608050/1‑94 (MQ=60)

                  CTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAG                                                                             >  SRR4302535.1972912/1‑94 (MQ=60)

                   TGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGG                                                                            <  SRR4302535.3503433/94‑1 (MQ=60)

                   TGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGG                                                                            >  SRR4302535.1309563/1‑94 (MQ=60)

                   TGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGG                                                                            <  SRR4302535.3136468/94‑1 (MQ=60)

                   TGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGG                                                                            >  SRR4302535.1419154/1‑94 (MQ=60)

                   TGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGG                                                                            <  SRR4302535.3767252/94‑1 (MQ=60)

                    GTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGT                                                                           <  SRR4302535.3403935/94‑1 (MQ=60)

                    GTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGT                                                                           >  SRR4302535.4691367/1‑94 (MQ=60)

                    GTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGT                                                                           >  SRR4302535.2491995/1‑94 (MQ=60)

                     TAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTT                                                                          <  SRR4302535.3641531/94‑1 (MQ=60)

                     TAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTT                                                                          <  SRR4302535.4600106/94‑1 (MQ=60)

                      AAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTT                                                                         >  SRR4302535.3810199/1‑94 (MQ=60)

                      AAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTT                                                                         <  SRR4302535.3577250/94‑1 (MQ=60)

                       AGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTC                                                                        >  SRR4302535.4700433/1‑94 (MQ=60)

                       AGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTC                                                                        <  SRR4302535.3680583/94‑1 (MQ=60)

                       AGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTC                                                                        <  SRR4302535.1606470/94‑1 (MQ=60)

                        GCTTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCG                                                                       <  SRR4302535.1140242/94‑1 (MQ=60)

                        GCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCG                                                                       <  SRR4302535.915192/94‑1 (MQ=60)

                        GCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCG                                                                       <  SRR4302535.709887/94‑1 (MQ=60)

                        GCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCG                                                                       >  SRR4302535.3057862/1‑94 (MQ=60)

                         CGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGA                                                                      >  SRR4302535.3198826/1‑94 (MQ=60)

                          GTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAG                                                                     <  SRR4302535.2317295/94‑1 (MQ=60)

                          GTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAG                                                                     <  SRR4302535.1890482/94‑1 (MQ=60)

                          GTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAG                                                                     <  SRR4302535.2732878/94‑1 (MQ=60)

                           TTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGG                                                                    >  SRR4302535.963638/1‑94 (MQ=60)

                           TTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGG                                                                    <  SRR4302535.1821228/94‑1 (MQ=60)

                              AACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCC                                                                 >  SRR4302535.1694384/1‑94 (MQ=60)

                               ACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCA                                                                >  SRR4302535.2106786/1‑94 (MQ=60)

                               ACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCA                                                                >  SRR4302535.3542947/1‑94 (MQ=60)

                                 CAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTTGAGGTCCACA                                                              >  SRR4302535.1298178/1‑94 (MQ=60)

                                 CAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACA                                                              >  SRR4302535.1952133/1‑94 (MQ=60)

                                 CAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACA                                                              >  SRR4302535.872281/1‑94 (MQ=60)

                                  AACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACAT                                                             <  SRR4302535.4427995/94‑1 (MQ=60)

                                  AACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACAT                                                             >  SRR4302535.3423196/1‑94 (MQ=60)

                                  AACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACAT                                                             >  SRR4302535.3231155/1‑94 (MQ=60)

                                    CCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCC                                                           <  SRR4302535.2170678/94‑1 (MQ=60)

                                        TTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTA                                                       >  SRR4302535.4459382/1‑94 (MQ=60)

                                        TTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTA                                                       >  SRR4302535.533936/1‑94 (MQ=60)

                                          CGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGC                                                     >  SRR4302535.4464322/1‑94 (MQ=60)

                                           GGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCA                                                    >  SRR4302535.1742453/1‑94 (MQ=60)

                                           GGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTACGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCA                                                    >  SRR4302535.2705766/1‑94 (MQ=60)

                                            GTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAG                                                   >  SRR4302535.4133538/1‑94 (MQ=60)

                                            GTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAG                                                   <  SRR4302535.4065501/94‑1 (MQ=60)

                                              AGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGTATGTGCGTTGCGCTGCTCATTCAGGAAGGTTGCGAGGTCCACATCCGGTAGCAGAT                                                 <  SRR4302535.2098669/94‑1 (MQ=60)

                                              AGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGAT                                                 >  SRR4302535.4277554/1‑94 (MQ=60)

                                              AGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGAT                                                 >  SRR4302535.3333692/1‑94 (MQ=60)

                                              AGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGAT                                                 >  SRR4302535.2580993/1‑94 (MQ=60)

                                               GATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATT                                                <  SRR4302535.688733/94‑1 (MQ=60)

                                               GATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATT                                                <  SRR4302535.605575/94‑1 (MQ=60)

                                               GATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATT                                                <  SRR4302535.658951/94‑1 (MQ=60)

                                                ATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTG                                               <  SRR4302535.398132/94‑1 (MQ=60)

                                                ATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTG                                               <  SRR4302535.2841504/94‑1 (MQ=60)

                                                 TGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGA                                              >  SRR4302535.3573259/1‑94 (MQ=60)

                                                  GAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGAT                                             <  SRR4302535.2575108/94‑1 (MQ=60)

                                                  GAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGAT                                             <  SRR4302535.754420/94‑1 (MQ=60)

                                                   AACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATG                                            >  SRR4302535.3137059/1‑94 (MQ=60)

                                                   AACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATG                                            >  SRR4302535.4341327/1‑94 (MQ=60)

                                                     CCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCT                                          <  SRR4302535.4461427/94‑1 (MQ=60)

                                                     CCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCT                                          >  SRR4302535.3280672/1‑94 (MQ=60)

                                                      CGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTG                                         <  SRR4302535.2632135/94‑1 (MQ=60)

                                                      CGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTG                                         <  SRR4302535.141015/94‑1 (MQ=60)

                                                      CGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTG                                         >  SRR4302535.1559566/1‑94 (MQ=60)

                                                      CGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTG                                         >  SRR4302535.2033724/1‑94 (MQ=60)

                                                       GCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGCAGCAGACTGATGCTGA                                        >  SRR4302535.688942/1‑94 (MQ=60)

                                                        CCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGAC                                       <  SRR4302535.2641930/94‑1 (MQ=60)

                                                        CCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGAC                                       >  SRR4302535.888844/1‑94 (MQ=60)

                                                            TGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAAT                                   <  SRR4302535.4370876/94‑1 (MQ=60)

                                                             GTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATT                                  <  SRR4302535.1114374/94‑1 (MQ=60)

                                                              TGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGCTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTC                                 >  SRR4302535.2381394/1‑94 (MQ=60)

                                                               GTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCC                                >  SRR4302535.4078272/1‑94 (MQ=60)

                                                                 TTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCC                              <  SRR4302535.3928760/94‑1 (MQ=60)

                                                                  TTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGCTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGAACAATTCCCCC                             >  SRR4302535.651973/1‑94 (MQ=60)

                                                                   TTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCG                            >  SRR4302535.1599421/1‑94 (MQ=60)

                                                                   TTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCG                            >  SRR4302535.227300/1‑94 (MQ=60)

                                                                   TTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCG                            <  SRR4302535.937735/94‑1 (MQ=60)

                                                                     CAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGT                          >  SRR4302535.305686/1‑94 (MQ=60)

                                                                     CAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGT                          <  SRR4302535.3590626/94‑1 (MQ=60)

                                                                      AGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTT                         <  SRR4302535.4475725/94‑1 (MQ=60)

                                                                      AGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTT                         <  SRR4302535.818466/94‑1 (MQ=60)

                                                                       GGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTG                        <  SRR4302535.1045862/94‑1 (MQ=60)

                                                                         CTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCC                      >  SRR4302535.3510499/1‑94 (MQ=60)

                                                                         CTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCC                      >  SRR4302535.2258774/1‑94 (MQ=60)

                                                                         CTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCC                      >  SRR4302535.1938235/1‑94 (MQ=60)

                                                                         CTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCC                      >  SRR4302535.39329/1‑94 (MQ=60)

                                                                         CTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCC                      <  SRR4302535.343661/94‑1 (MQ=60)

                                                                         CTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTAATGCTGACAAATTCCCCAGGTTGCC                      >  SRR4302535.3076459/1‑94 (MQ=60)

                                                                         CTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGCAGCAGATTGATGCTGACAAATTCCCCCGCTTGCC                      >  SRR4302535.1314347/1‑94 (MQ=60)

                                                                          TTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCA                     <  SRR4302535.4285833/94‑1 (MQ=60)

                                                                           TTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAG                    <  SRR4302535.4359842/94‑1 (MQ=60)

                                                                           TTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAG                    <  SRR4302535.1879837/94‑1 (MQ=60)

                                                                            TGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGT                   >  SRR4302535.3846604/1‑94 (MQ=60)

                                                                            TGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGT                   >  SRR4302535.3373986/1‑94 (MQ=60)

                                                                               TTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGC                >  SRR4302535.3503509/1‑94 (MQ=60)

                                                                               TTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGC                <  SRR4302535.2042438/94‑1 (MQ=60)

                                                                                  GGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTG             <  SRR4302535.1937448/94‑1 (MQ=60)

                                                                                    ATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAA           >  SRR4302535.2855526/1‑94 (MQ=60)

                                                                                    ATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAA           <  SRR4302535.4519751/94‑1 (MQ=60)

                                                                                     TGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAA          >  SRR4302535.2090033/1‑94 (MQ=60)

                                                                                      GTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAAT         >  SRR4302535.13323/1‑94 (MQ=60)

                                                                                       TGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATC        <  SRR4302535.3852284/94‑1 (MQ=60)

                                                                                        GCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCT       <  SRR4302535.3994824/94‑1 (MQ=60)

                                                                                         CGTTGCGCTGCTCATTCAGGAAGGTTTCAAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGC‑TGAAATCTGC     >  SRR4302535.712348/1‑94 (MQ=60)

                                                                                         CGTTGCGATGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCTGATGCCAGTAGCTTGATATCTG      >  SRR4302535.1558416/1‑94 (MQ=60)

                                                                                          GTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGC     >  SRR4302535.4105859/1‑94 (MQ=60)

                                                                                            TGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAA   >  SRR4302535.4496715/1‑94 (MQ=60)

                                                                                             GCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAAC  <  SRR4302535.3577876/94‑1 (MQ=60)

CGGGATTTGCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTACGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAAC  >  NC_000913/3634135‑3634321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`