Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,670,614 C→T R215C (CGT→TGT)  yhjC → putative transcriptional regulator LYSR‑type

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,670,6140CT99.6% 923.0 / NA 258R215C (CGT→TGT) yhjCputative transcriptional regulator LYSR‑type
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (130/127);  total (131/127)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 268 total aligned reads displayed.

CTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTG  >  NC_000913/3670524‑3670703
                                                                                          |                                                                                         
cTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAgtgtgt                                                                                         >  2:4246206/1‑93 (MQ=255)
cTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAgtgtgt                                                                                         <  2:3619255/93‑1 (MQ=255)
  gACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTta                                                                                       <  2:2457176/93‑1 (MQ=255)
  gACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTta                                                                                       <  1:2046767/93‑1 (MQ=255)
   aCCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTtat                                                                                      >  2:4453806/1‑93 (MQ=255)
   aCCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTtat                                                                                      <  1:3221035/93‑1 (MQ=255)
    ccATGGTCATCTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTtata                                                                                     <  2:2054616/93‑1 (MQ=255)
    ccATGGTCAGCTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTtata                                                                                     <  2:2410426/93‑1 (MQ=255)
    ccATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTtata                                                                                     >  2:3878028/1‑93 (MQ=255)
      aTGGTCAACTGTGCCAGTCCGCACTATCTGACGAGCTTTTGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATaca                                                                                   >  2:2239658/1‑93 (MQ=255)
        ggTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACAcc                                                                                 <  2:576452/93‑1 (MQ=255)
        ggTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACAcc                                                                                 <  1:1274229/93‑1 (MQ=255)
          tCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGc                                                                               >  1:2203319/1‑93 (MQ=255)
           cAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCa                                                                              <  1:4495084/93‑1 (MQ=255)
           cAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCa                                                                              <  1:658077/93‑1 (MQ=255)
             aCTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCAcc                                                                            >  2:481602/1‑93 (MQ=255)
             aCTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCAcc                                                                            <  1:3258676/93‑1 (MQ=255)
              cTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCt                                                                           >  2:3997056/1‑93 (MQ=255)
                gtgCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTgg                                                                         <  1:3828714/93‑1 (MQ=255)
                 tgCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTggg                                                                        >  1:1994893/1‑93 (MQ=255)
                 tgCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTggg                                                                        >  2:4182855/1‑93 (MQ=255)
                 tgCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTggg                                                                        >  1:4426098/1‑93 (MQ=255)
                  gCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGgt                                                                       <  1:99267/93‑1 (MQ=255)
                   ccAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGgtg                                                                      >  2:3277119/1‑93 (MQ=255)
                   ccAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGgtg                                                                      <  2:3131750/93‑1 (MQ=255)
                   ccAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCAGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGgtg                                                                      <  1:2685461/93‑1 (MQ=255)
                     aGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTa                                                                    >  2:143702/1‑93 (MQ=255)
                      gTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTAc                                                                   <  2:1182614/93‑1 (MQ=255)
                      gTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTAc                                                                   >  1:419740/1‑93 (MQ=255)
                       tCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACa                                                                  >  2:2287643/1‑93 (MQ=255)
                         cGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATc                                                                <  2:2149544/93‑1 (MQ=255)
                          gCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATcc                                                               >  2:121455/1‑93 (MQ=255)
                          gCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATcc                                                               >  2:157394/1‑93 (MQ=255)
                            aCTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGt                                                             >  2:3749157/1‑93 (MQ=255)
                            aCTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGt                                                             >  2:3512988/1‑93 (MQ=255)
                              tATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACAGCGCACCTGGGTGTACATCCGTTa                                                           >  1:1492151/1‑93 (MQ=255)
                              tATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTa                                                           >  1:1399290/1‑93 (MQ=255)
                              tATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTa                                                           >  1:2615750/1‑93 (MQ=255)
                              tATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTa                                                           <  2:2943056/93‑1 (MQ=255)
                               aTCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCCCCTGGGTGTACATCCGTTAg                                                          <  2:4427955/93‑1 (MQ=255)
                                tCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAgg                                                         >  1:3848463/1‑93 (MQ=255)
                                 cTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGt                                                        <  2:1936009/93‑1 (MQ=255)
                                  tGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGtt                                                       >  1:1568187/1‑93 (MQ=255)
                                      gcgcTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGa                                                   <  1:1523867/93‑1 (MQ=255)
                                      gcgcTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGa                                                   <  1:757159/93‑1 (MQ=255)
                                       cgcTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAg                                                  <  1:301355/93‑1 (MQ=255)
                                        gcTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAgg                                                 <  2:857761/93‑1 (MQ=255)
                                        gcTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAgg                                                 >  1:1123470/1‑93 (MQ=255)
                                         cTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGt                                                >  1:3408307/1‑93 (MQ=255)
                                          tttGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCTCCTGGGTGTACATCCGTTAGGTTTTGAGGtt                                               <  1:2266466/93‑1 (MQ=255)
                                          tttGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGtt                                               >  1:2783067/1‑93 (MQ=255)
                                               ttATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAg                                          <  1:3358326/93‑1 (MQ=255)
                                                  tCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGt                                       <  2:2718417/93‑1 (MQ=255)
                                                  tCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGt                                       >  2:4555393/1‑93 (MQ=255)
                                                  tCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGt                                       <  1:3503030/93‑1 (MQ=255)
                                                  tCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGt                                       >  1:178156/1‑93 (MQ=255)
                                                    cGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTa                                     >  1:4394453/1‑93 (MQ=255)
                                                     gCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTaa                                    >  1:4616769/1‑93 (MQ=255)
                                                     gCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTaa                                    >  1:802638/1‑93 (MQ=255)
                                                      cAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAAt                                   <  2:3408456/93‑1 (MQ=255)
                                                       aaaGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATg                                  >  1:2407276/1‑93 (MQ=255)
                                                        aaGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATgg                                 >  1:2422174/1‑93 (MQ=255)
                                                         aGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGc                                <  2:1808813/93‑1 (MQ=255)
                                                            cccGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTc                             >  1:4360465/1‑93 (MQ=255)
                                                             ccGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTcc                            >  1:1834143/1‑93 (MQ=255)
                                                             ccGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTcc                            >  2:1145220/1‑93 (MQ=255)
                                                              cGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCa                           >  1:1341238/1‑93 (MQ=255)
                                                              cGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCa                           >  2:4648358/1‑93 (MQ=255)
                                                               gatgatCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAg                          >  1:2242792/1‑93 (MQ=255)
                                                                atgatCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGt                         >  2:2675985/1‑93 (MQ=255)
                                                                 tgatCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTg                        >  1:917332/1‑93 (MQ=255)
                                                                 tgatCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTg                        >  1:1208297/1‑93 (MQ=255)
                                                                 tgatCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTg                        <  1:4186536/93‑1 (MQ=255)
                                                                 tgatCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTg                        <  1:1973639/93‑1 (MQ=255)
                                                                  gatCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTgg                       >  1:2831689/1‑93 (MQ=255)
                                                                   atCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGt                      <  2:1699173/93‑1 (MQ=255)
                                                                   atCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGt                      >  2:3567090/1‑93 (MQ=255)
                                                                    tCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGtt                     >  2:1062381/1‑93 (MQ=255)
                                                                    tCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGtt                     >  1:1901572/1‑93 (MQ=255)
                                                                     cTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGttt                    <  1:1075637/93‑1 (MQ=255)
                                                                       gACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTaa                  >  2:2578898/1‑93 (MQ=255)
                                                                       gACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTaa                  <  1:3928393/93‑1 (MQ=255)
                                                                        aCTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAg                 <  1:2089084/93‑1 (MQ=255)
                                                                        aCTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAg                 <  2:2803284/93‑1 (MQ=255)
                                                                          ttCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTc               >  2:4113355/1‑93 (MQ=255)
                                                                          ttCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTc               >  1:4253000/1‑93 (MQ=255)
                                                                             acacGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTgg            >  2:4045037/1‑93 (MQ=255)
                                                                             acacGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTgg            <  2:2990252/93‑1 (MQ=255)
                                                                              cacGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTggc           <  1:172401/93‑1 (MQ=255)
                                                                               acGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTggcg          >  2:2364347/1‑93 (MQ=255)
                                                                               acGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTggcg          >  2:2209246/1‑93 (MQ=255)
                                                                                cGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTggcgg         >  1:4451875/1‑93 (MQ=255)
                                                                                 gCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTggcggc        >  2:4313504/1‑93 (MQ=255)
                                                                                   aaTAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCAt      >  2:1259084/1‑93 (MQ=255)
                                                                                   aaTAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGATTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCAt      >  1:3516821/1‑93 (MQ=255)
                                                                                    aTAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATg     >  2:367640/1‑93 (MQ=255)
                                                                                    aTAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATg     <  1:2755193/93‑1 (MQ=255)
                                                                                    aTAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATg     >  1:2161223/1‑93 (MQ=255)
                                                                                      aGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGtt   <  1:2958382/93‑1 (MQ=255)
                                                                                      aGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGtt   <  1:4105103/93‑1 (MQ=255)
                                                                                          |                                                                                         
CTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTG  >  NC_000913/3670524‑3670703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGG  >  NC_000913/3670521‑3670707
                                                                                             |                                                                                             
AAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGT                                                                                               <  SRR4302535.1699937/94‑1 (MQ=60)
AAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGT                                                                                               >  SRR4302535.2033463/1‑94 (MQ=60)
AAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGT                                                                                               >  SRR4302535.2825009/1‑94 (MQ=60)
AAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGT                                                                                               <  SRR4302535.2158495/94‑1 (MQ=60)
 AACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTAATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTG                                                                                              <  SRR4302535.3328474/94‑1 (MQ=60)
  ACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGT                                                                                             >  SRR4302535.998895/1‑94 (MQ=60)
  ACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGT                                                                                             >  SRR4302535.2949761/1‑94 (MQ=60)
     GACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTAT                                                                                          <  SRR4302535.2061505/94‑1 (MQ=60)
     GACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTAT                                                                                          <  SRR4302535.1871673/94‑1 (MQ=60)
     GACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTAT                                                                                          <  SRR4302535.1871674/94‑1 (MQ=60)
      ACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATA                                                                                         <  SRR4302535.3243357/94‑1 (MQ=60)
      ACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATA                                                                                         >  SRR4302535.4654478/1‑94 (MQ=60)
       CCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATAC                                                                                        >  SRR4302535.516721/1‑94 (MQ=60)
       CCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATAC                                                                                        <  SRR4302535.3559911/94‑1 (MQ=60)
        CATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACA                                                                                       >  SRR4302535.2403569/1‑94 (MQ=60)
         ATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACAC                                                                                      <  SRR4302535.3530826/94‑1 (MQ=60)
          TGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACC                                                                                     <  SRR4302535.1796642/94‑1 (MQ=60)
           GGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCG                                                                                    <  SRR4302535.2265736/94‑1 (MQ=60)
           GGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCG                                                                                    <  SRR4302535.1283325/94‑1 (MQ=60)
            GTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGC                                                                                   >  SRR4302535.2219019/1‑94 (MQ=60)
             TCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCA                                                                                  <  SRR4302535.4129872/94‑1 (MQ=60)
             TCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCA                                                                                  <  SRR4302535.1270315/94‑1 (MQ=60)
              CAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCAC                                                                                 >  SRR4302535.3985534/1‑94 (MQ=60)
              CAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCAC                                                                                 >  SRR4302535.4264456/1‑94 (MQ=60)
              CAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCAC                                                                                 <  SRR4302535.4525937/94‑1 (MQ=60)
              CAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCAC                                                                                 <  SRR4302535.662670/94‑1 (MQ=60)
               AACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACC                                                                                <  SRR4302535.1714972/94‑1 (MQ=60)
                ACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCT                                                                               <  SRR4302535.3281324/94‑1 (MQ=60)
                ACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCT                                                                               <  SRR4302535.3941600/94‑1 (MQ=60)
                  CGTGCCAGTCCGCACTTTCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGG                                                                             <  SRR4302535.1528191/94‑1 (MQ=60)
                   GTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGG                                                                            >  SRR4302535.2009258/1‑94 (MQ=60)
                   GTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGG                                                                            <  SRR4302535.3855123/94‑1 (MQ=60)
                   GTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGG                                                                            >  SRR4302535.4456416/1‑94 (MQ=60)
                   GTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGG                                                                            <  SRR4302535.1370170/94‑1 (MQ=60)
                    TGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGT                                                                           <  SRR4302535.873577/94‑1 (MQ=60)
                    TGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGT                                                                           <  SRR4302535.436330/94‑1 (MQ=60)
                     GCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTG                                                                          <  SRR4302535.1383691/94‑1 (MQ=60)
                     GCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTG                                                                          <  SRR4302535.99966/94‑1 (MQ=60)
                      CCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGT                                                                         <  SRR4302535.2178179/94‑1 (MQ=60)
                      CCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCAGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGT                                                                         <  SRR4302535.2704663/94‑1 (MQ=60)
                        AGTCCGCACTATCTGACGCTCTTTGGTTATCCGCAAATCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTAC                                                                       <  SRR4302535.4597472/94‑1 (MQ=60)
                        AGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTAC                                                                       >  SRR4302535.422674/1‑94 (MQ=60)
                        AGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTAC                                                                       <  SRR4302535.2084849/94‑1 (MQ=60)
                         GTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACA                                                                      <  SRR4302535.4224339/94‑1 (MQ=60)
                          TCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACAT                                                                     >  SRR4302535.1921339/1‑94 (MQ=60)
                             GCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCG                                                                  >  SRR4302535.2552072/1‑94 (MQ=60)
                               ACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTT                                                                <  SRR4302535.3053771/94‑1 (MQ=60)
                                CTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACAGCGCACCTGGGTGTACATCCGTTA                                                               >  SRR4302535.1502868/1‑94 (MQ=60)
                                CTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTA                                                               >  SRR4302535.2634507/1‑94 (MQ=60)
                                CTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTA                                                               >  SRR4302535.1409279/1‑94 (MQ=60)
                                 TATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAG                                                              >  SRR4302535.2624403/1‑94 (MQ=60)
                                 TATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAG                                                              <  SRR4302535.3027027/94‑1 (MQ=60)
                                  ATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGT                                                             >  SRR4302535.3617173/1‑94 (MQ=60)
                                  ATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGG                                                             >  SRR4302535.3874952/1‑94 (MQ=60)
                                  ATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGG                                                             <  SRR4302535.2809436/94‑1 (MQ=60)
                                  ATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGG                                                             <  SRR4302535.1693047/94‑1 (MQ=60)
                                   TCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGT                                                            <  SRR4302535.2803525/94‑1 (MQ=60)
                                   TCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGT                                                            >  SRR4302535.1756970/1‑94 (MQ=60)
                                    CTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCTCCTGTGTGTACATCCGTTAGGTT                                                           <  SRR4302535.1993742/94‑1 (MQ=60)
                                    CTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTT                                                           >  SRR4302535.1579504/1‑94 (MQ=60)
                                    CTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTT                                                           >  SRR4302535.888905/1‑94 (MQ=60)
                                     TGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTT                                                          >  SRR4302535.1262966/1‑94 (MQ=60)
                                      GACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTT                                                         >  SRR4302535.4474149/1‑94 (MQ=60)
                                       ACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTG                                                        <  SRR4302535.3395742/94‑1 (MQ=60)
                                         GCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAG                                                      <  SRR4302535.762487/94‑1 (MQ=60)
                                         GCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAG                                                      <  SRR4302535.1534847/94‑1 (MQ=60)
                                          CGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGG                                                     <  SRR4302535.303455/94‑1 (MQ=60)
                                          CGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGG                                                     >  SRR4302535.1131487/1‑94 (MQ=60)
                                           GCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGT                                                    <  SRR4302535.2674740/94‑1 (MQ=60)
                                           GCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGT                                                    >  SRR4302535.3432032/1‑94 (MQ=60)
                                            CTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTT                                                   <  SRR4302535.1410036/94‑1 (MQ=60)
                                            CTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTT                                                   >  SRR4302535.2012460/1‑94 (MQ=60)
                                            CTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTT                                                   >  SRR4302535.2802873/1‑94 (MQ=60)
                                             TTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCTCCTGGGTGTACATCCGTTAGGTTTTGAGGTTG                                                  <  SRR4302535.2282619/94‑1 (MQ=60)
                                              TTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGC                                                 >  SRR4302535.1549796/1‑94 (MQ=60)
                                                GGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCA                                               <  SRR4302535.4453462/94‑1 (MQ=60)
                                                 GTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAG                                              >  SRR4302535.2939475/1‑94 (MQ=60)
                                                 GTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGAGTGTACATCCGTTAGGTTTTGAGGTTGCCAG                                              <  SRR4302535.4497727/94‑1 (MQ=60)
                                                  TTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGC                                             <  SRR4302535.3381705/94‑1 (MQ=60)
                                                   TATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCG                                            <  SRR4302535.420895/94‑1 (MQ=60)
                                                   TATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCG                                            >  SRR4302535.24391/1‑94 (MQ=60)
                                                   TATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCG                                            <  SRR4302535.420893/94‑1 (MQ=60)
                                                    ATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGT                                           >  SRR4302535.179394/1‑94 (MQ=60)
                                                     TCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTT                                          <  SRR4302535.3527378/94‑1 (MQ=60)
                                                      CCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTA                                         >  SRR4302535.4424525/1‑94 (MQ=60)
                                                       CGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAA                                        >  SRR4302535.4648145/1‑94 (MQ=60)
                                                       CGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAA                                        >  SRR4302535.808276/1‑94 (MQ=60)
                                                        GCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAAT                                       <  SRR4302535.1496925/94‑1 (MQ=60)
                                                        GCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAAT                                       >  SRR4302535.346214/1‑94 (MQ=60)
                                                         CAAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATG                                      >  SRR4302535.2424604/1‑94 (MQ=60)
                                                          AAAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGG                                     >  SRR4302535.2439620/1‑94 (MQ=60)
                                                           AAGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGC                                    >  SRR4302535.447740/1‑94 (MQ=60)
                                                            AGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCG                                   >  SRR4302535.3857980/1‑94 (MQ=60)
                                                            AGCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCG                                   >  SRR4302535.970014/1‑94 (MQ=60)
                                                             GCCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGT                                  >  SRR4302535.4418183/1‑94 (MQ=60)
                                                              CCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTC                                 >  SRR4302535.3414464/1‑94 (MQ=60)
                                                              CCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTC                                 >  SRR4302535.4390308/1‑94 (MQ=60)
                                                              CCCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTC                                 >  SRR4302535.2021246/1‑94 (MQ=60)
                                                               CCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCC                                >  SRR4302535.1847377/1‑94 (MQ=60)
                                                               CCCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCC                                >  SRR4302535.4646310/1‑94 (MQ=60)
                                                                CCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCA                               >  SRR4302535.4432351/1‑94 (MQ=60)
                                                                CCGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCA                               >  SRR4302535.1350805/1‑94 (MQ=60)
                                                                 CGATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAG                              >  SRR4302535.2258745/1‑94 (MQ=60)
                                                                 CGATGATCTGACGTCACACGCAATAGTGTGTTATACTCCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAG                              <  SRR4302535.729817/94‑1 (MQ=60)
                                                                  GATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGT                             >  SRR4302535.3034739/1‑94 (MQ=60)
                                                                   ATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCTAGTG                            >  SRR4302535.2107497/1‑94 (MQ=60)
                                                                   ATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTG                            >  SRR4302535.923839/1‑94 (MQ=60)
                                                                   ATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTG                            >  SRR4302535.2731770/1‑94 (MQ=60)
                                                                   ATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTG                            >  SRR4302535.1216982/1‑94 (MQ=60)
                                                                   ATGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTG                            >  SRR4302535.2107500/1‑94 (MQ=60)
                                                                    TGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGG                           >  SRR4302535.3870490/1‑94 (MQ=60)
                                                                    TGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGG                           >  SRR4302535.2851788/1‑94 (MQ=60)
                                                                    TGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGG                           <  SRR4302535.1987853/94‑1 (MQ=60)
                                                                    TGATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGG                           <  SRR4302535.4215188/94‑1 (MQ=60)
                                                                     GATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGT                          >  SRR4302535.2730069/1‑94 (MQ=60)
                                                                      ATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTT                         >  SRR4302535.1915259/1‑94 (MQ=60)
                                                                      ATCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTT                         >  SRR4302535.773110/1‑94 (MQ=60)
                                                                       TCTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGCTGCCAGCGTTAATGGCGTCCAGTGGTTT                        >  SRR4302535.1798041/1‑94 (MQ=60)
                                                                        CTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTA                       >  SRR4302535.1142893/1‑94 (MQ=60)
                                                                        CTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTA                       <  SRR4302535.3488871/94‑1 (MQ=60)
                                                                        CTGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTA                       <  SRR4302535.1083284/94‑1 (MQ=60)
                                                                         TGACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGCTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAA                      >  SRR4302535.278686/1‑94 (MQ=60)
                                                                          GACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAG                     <  SRR4302535.3955226/94‑1 (MQ=60)
                                                                          GACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAG                     >  SRR4302535.1183559/1‑94 (MQ=60)
                                                                           ACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGT                    <  SRR4302535.2104137/94‑1 (MQ=60)
                                                                           ACTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGT                    <  SRR4302535.1107787/94‑1 (MQ=60)
                                                                            CTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTC                   >  SRR4302535.4308733/1‑94 (MQ=60)
                                                                            CTTCACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTC                   >  SRR4302535.4282142/1‑94 (MQ=60)
                                                                               CACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGG                <  SRR4302535.1551164/94‑1 (MQ=60)
                                                                                ACACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGC               >  SRR4302535.1988448/1‑94 (MQ=60)
                                                                                 CACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCG              <  SRR4302535.2258840/94‑1 (MQ=60)
                                                                                 CACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCG              <  SRR4302535.173596/94‑1 (MQ=60)
                                                                                  ACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGG             >  SRR4302535.4482405/1‑94 (MQ=60)
                                                                                  ACGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGGTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGG             >  SRR4302535.3254545/1‑94 (MQ=60)
                                                                                   CGCAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGC            >  SRR4302535.3367059/1‑94 (MQ=60)
                                                                                     CAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCAT          >  SRR4302535.700450/1‑94 (MQ=60)
                                                                                     CAATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGATTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCAT          >  SRR4302535.3541255/1‑94 (MQ=60)
                                                                                      AATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATG         >  SRR4302535.2243259/1‑94 (MQ=60)
                                                                                      AATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATG         >  SRR4302535.1318857/1‑94 (MQ=60)
                                                                                      AATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATG         >  SRR4302535.2176737/1‑94 (MQ=60)
                                                                                      AATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATG         >  SRR4302535.2176738/1‑94 (MQ=60)
                                                                                       ATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGT        <  SRR4302535.4249561/94‑1 (MQ=60)
                                                                                       ATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGT        <  SRR4302535.2774833/94‑1 (MQ=60)
                                                                                       ATAGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGT        >  SRR4302535.2408497/1‑94 (MQ=60)
                                                                                         AGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTG      <  SRR4302535.4133208/94‑1 (MQ=60)
                                                                                         AGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTG      >  SRR4302535.2683013/1‑94 (MQ=60)
                                                                                         AGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTG      <  SRR4302535.3253719/94‑1 (MQ=60)
                                                                                         AGTGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTG      <  SRR4302535.2979158/94‑1 (MQ=60)
                                                                                           TGTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGAC    <  SRR4302535.3243964/94‑1 (MQ=60)
                                                                                            GTGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACG   <  SRR4302535.1035055/94‑1 (MQ=60)
                                                                                             TGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGG  >  SRR4302535.751567/1‑94 (MQ=60)
                                                                                             |                                                                                             
AAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGG  >  NC_000913/3670521‑3670707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑