Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,674,040	A→G	G652G (GGT→GGC)	yhjG ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,674,040	0	A	G	98.6%	657.9 / ‑4.7	217	G652G (GGT→GGC)	yhjG	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (1/2); new base G (81/133); total (82/135)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 232 total aligned reads displayed.

CACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCACCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAACGCAGTGTGATAAT  >  NC_000913/3673951‑3674129

cacTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGcgcca                                                                                         <  1:2751386/93‑1 (MQ=255)

cacTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGcgcca                                                                                         >  2:2376929/1‑93 (MQ=255)

cacTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGcgcca                                                                                         <  1:2828516/93‑1 (MQ=255)

cacTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGcgcca                                                                                         >  2:2979593/1‑93 (MQ=255)

 acTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGcgccac                                                                                        <  2:372138/93‑1 (MQ=255)

     aTTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGc                                                                                    <  2:4382468/93‑1 (MQ=255)

       tAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCaa                                                                                  >  1:575128/1‑93 (MQ=255)

         gCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATc                                                                                <  2:1938806/93‑1 (MQ=255)

         gCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATc                                                                                <  2:2791985/93‑1 (MQ=255)

          ccTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCa                                                                               <  2:3174253/93‑1 (MQ=255)

           cTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAg                                                                              >  1:1420240/1‑93 (MQ=255)

           cTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAg                                                                              >  1:1734388/1‑93 (MQ=255)

           cTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAg                                                                              >  2:154048/1‑93 (MQ=255)

            tCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGc                                                                             >  1:4255104/1‑93 (MQ=255)

            tCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGc                                                                             <  2:2255066/93‑1 (MQ=255)

              cccTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCgg                                                                           >  2:1344910/1‑93 (MQ=255)

               ccTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGt                                                                          <  1:4571045/93‑1 (MQ=255)

                cTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTc                                                                         <  1:3173868/93‑1 (MQ=255)

                cTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTc                                                                         <  1:363898/93‑1 (MQ=255)

                 ttCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTcc                                                                        <  2:1987758/93‑1 (MQ=255)

                 ttCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTcc                                                                        <  1:161065/93‑1 (MQ=255)

                   cGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCgg                                                                      <  2:1870808/93‑1 (MQ=255)

                    ggAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGc                                                                     >  1:2999977/1‑93 (MQ=255)

                    ggAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGc                                                                     <  1:4185689/93‑1 (MQ=255)

                      aaGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCtt                                                                   >  1:3322843/1‑93 (MQ=255)

                       aGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCttt                                                                  >  2:2076814/1‑93 (MQ=255)

                       aGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCttt                                                                  <  1:3953054/93‑1 (MQ=255)

                        ggTGAGATCGGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTc                                                                 <  2:3274334/93‑1 (MQ=255)

                        ggTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTc                                                                 >  2:334521/1‑93 (MQ=255)

                        ggTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTc                                                                 >  2:867959/1‑93 (MQ=255)

                           gagaTCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCAcc                                                              <  1:867051/93‑1 (MQ=255)

                           gagaTCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCAcc                                                              >  2:2243471/1‑93 (MQ=255)

                           gagaTCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCAcc                                                              >  2:2497497/1‑93 (MQ=255)

                            agaTCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCAccc                                                             >  2:2628225/1‑93 (MQ=255)

                             gaTCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCAcccc                                                            >  1:3457149/1‑93 (MQ=255)

                             gaTCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCAcccc                                                            <  2:632048/93‑1 (MQ=255)

                              aTCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCa                                                           <  2:2711596/93‑1 (MQ=255)

                              aTCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCa                                                           <  1:4280671/93‑1 (MQ=255)

                              aTCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCa                                                           <  1:1525027/93‑1 (MQ=255)

                                 aGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGcc                                                        <  1:1792386/93‑1 (MQ=255)

                                 aGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGcc                                                        <  1:673141/93‑1 (MQ=255)

                                  gTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCt                                                       <  1:896725/93‑1 (MQ=255)

                                    gCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑c                                                    <  2:1046164/93‑1 (MQ=255)

                                     ccAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑Cg                                                   <  2:3183859/93‑1 (MQ=255)

                                       aGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGa                                                 >  1:2309195/1‑93 (MQ=255)

                                        gTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGAt                                                <  1:103812/93‑1 (MQ=255)

                                        gTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGAt                                                >  2:1377352/1‑93 (MQ=255)

                                          aaCGCCGCCGCCGGAGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGAttt                                              >  1:2026522/1‑93 (MQ=255)

                                           aCGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGAtttt                                             <  2:1234791/93‑1 (MQ=255)

                                           aCGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGAtttt                                             >  1:1555696/1‑93 (MQ=255)

                                               cgccgccgGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTaaa                                         <  1:235163/93‑1 (MQ=255)

                                               cgccgccgGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTaaa                                         >  1:3072565/1‑93 (MQ=255)

                                               cgccgccgGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTaaa                                         <  1:1091650/93‑1 (MQ=255)

                                               cgccgccgGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTaaa                                         <  1:1324457/93‑1 (MQ=255)

                                                gccgctgGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGGCTG‑CGGATTTTTAAAc                                        <  2:777801/93‑1 (MQ=255)

                                                  cgccgGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTAAACGt                                      >  1:2480882/1‑93 (MQ=255)

                                                  cgccgGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTAAACGt                                      <  1:1249933/93‑1 (MQ=255)

                                                   gccgGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTAAACGTc                                     <  2:1263950/93‑1 (MQ=255)

                                                     cgGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTAAACGTccc                                   <  2:41137/93‑1 (MQ=255)

                                                     cgGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTAAACGTccc                                   <  2:4492040/93‑1 (MQ=255)

                                                     cgGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTAAACGTccc                                   >  2:3432510/1‑93 (MQ=255)

                                                      ggTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTAAACGTcccc                                  >  1:719104/1‑93 (MQ=255)

                                                      ggTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTAAACGTcccc                                  <  1:427412/93‑1 (MQ=255)

                                                         gtTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTAAACGTCCCCCGc                               >  1:2466586/1‑93 (MQ=255)

                                                          ttACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCTCCCCAGCCTG‑CGGATTTTTAAACGTCCCCCGca                              <  2:160683/93‑1 (MQ=255)

                                                          ttACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTAAACGTCCCCCGca                              <  1:1005731/93‑1 (MQ=255)

                                                           tACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTAAACGTCCCCCGcac                             >  2:1168895/1‑93 (MQ=255)

                                                             ccAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTAAACGTCCCCCGCACAt                           <  1:4638621/93‑1 (MQ=255)

                                                             ccAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTAAACGTCCCCCGCACAt                           <  1:4457671/93‑1 (MQ=255)

                                                              cAGCTTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTAAACGTCCCCCGCACATa                          <  1:1027343/93‑1 (MQ=255)

                                                              cAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTAAACGTCCCCCGCACATa                          <  2:3011265/93‑1 (MQ=255)

                                                               cgCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTAAACGTCCCCCGCACATAc                         <  1:393854/92‑1 (MQ=255)

                                                               aGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTAAACGTCCCCCGCACATAc                         <  2:3931795/93‑1 (MQ=255)

                                                               aGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTAAACGTCCCCCGCACATAc                         >  2:3900175/1‑93 (MQ=255)

                                                               aGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTAAACGTCCCCCGCACATAc                         <  1:1548004/93‑1 (MQ=255)

                                                               aGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTAAACGTCCCCCGCACATAc                         >  2:3846736/1‑93 (MQ=255)

                                                                 cGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTAAACGTCCCCCGCACATACAg                       >  2:2544583/1‑93 (MQ=255)

                                                                  gTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTAAACGTCCCCCGCACATACAgc                      <  1:4307834/93‑1 (MQ=255)

                                                                  gTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTAAACGTCCCCCGCACATACAgc                      >  2:616971/1‑93 (MQ=255)

                                                                      ccAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTAAACGTCCCCCGCACATACAgcggcg                  >  1:2687734/1‑93 (MQ=255)

                                                                      ccAGTGCCGCAGCAACAGCACCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTAAACGTCCCCCGCACATACAgcggcg                  >  2:530360/1‑93 (MQ=255)

                                                                       cAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTAAACGTCCCCCGCACATACAGCGGCGa                 >  2:3546913/1‑93 (MQ=255)

                                                                       cAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTAAACGTCCCCCGCACATACAGCGGCGa                 <  2:2886236/93‑1 (MQ=255)

                                                                        aGTGCCGCAGCAACAGCGCCCCGGGCAATCAGCGGGCCGGCTTTCAACCCCACCTGCCGGATTTTT‑AACGTCCCCCGCACATACAgcggcgga                >  2:4177082/1‑91 (MQ=255)

                                                                        aGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTAAACGTCCCCCGCACATACAGCGGCGaa                <  1:2550811/93‑1 (MQ=255)

                                                                        aGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTAAACGTCCCCCGCACATACAGCGGCGaa                <  2:3111191/93‑1 (MQ=255)

                                                                        aGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTAAACGTCCCCCGCACATACAGCGGCGaa                <  1:3903236/93‑1 (MQ=255)

                                                                         gTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAAc               <  2:915344/93‑1 (MQ=255)

                                                                         gTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAAc               <  2:1877007/93‑1 (MQ=255)

                                                                         gTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAAc               >  2:2452293/1‑93 (MQ=255)

                                                                          tGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAACg              >  2:1200074/1‑93 (MQ=255)

                                                                          tGCCGCAGCAACAGCACCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAACg              <  2:4169296/93‑1 (MQ=255)

                                                                            ccGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAACGCa            <  1:2200780/93‑1 (MQ=255)

                                                                            ccGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAACGCa            <  1:2040844/93‑1 (MQ=255)

                                                                              gcagcaACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAACGCAgt          >  2:4439169/1‑93 (MQ=255)

                                                                                   aaCAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAACGCAGTGTGAt     <  1:2101479/93‑1 (MQ=255)

                                                                                    aCAGCGCCACGGGGAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAACTCAGGGTGATa    >  2:3383513/1‑93 (MQ=255)

                                                                                    aCAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAACGCAGTGTGATa    >  1:764524/1‑93 (MQ=255)

                                                                                     cAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAACGCAGTGTGATaa   <  2:3081082/93‑1 (MQ=255)

                                                                                      aGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAACGCAGTGTGATAAt  <  1:3192668/93‑1 (MQ=255)

CACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCACCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG‑CGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAACGCAGTGTGATAAT  >  NC_000913/3673951‑3674129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCACCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAACGCAGTGTGATAATCCGA  >  NC_000913/3673951‑3674133

CACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCAC                                                                                           <  SRR4302535.2848590/94‑1 (MQ=60)

CACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCAC                                                                                           <  SRR4302535.2770998/94‑1 (MQ=60)

CACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCACCAC                                                                                           >  SRR4302535.2755318/1‑94 (MQ=60)

  CTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGG                                                                                         >  SRR4302535.512570/1‑94 (MQ=60)

  CTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGG                                                                                         <  SRR4302535.2338780/94‑1 (MQ=60)

   TGATTAGCCTCCCGTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGGCGGTGTTACCAGGTTTGCCAGTGCCGCAGCAACAGCGCCACGGG                                                                                        <  SRR4302535.1142260/94‑1 (MQ=60)

   TGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGG                                                                                        >  SRR4302535.96654/1‑94 (MQ=60)

      TTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAA                                                                                     >  SRR4302535.579165/1‑94 (MQ=60)

       TAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAAT                                                                                    >  SRR4302535.375627/1‑94 (MQ=60)

        AGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATC                                                                                   <  SRR4302535.888430/94‑1 (MQ=60)

         GCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCA                                                                                  <  SRR4302535.792811/94‑1 (MQ=60)

         GCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCA                                                                                  >  SRR4302535.1543661/1‑94 (MQ=60)

          CCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAG                                                                                 >  SRR4302535.1746924/1‑94 (MQ=60)

          CCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAG                                                                                 >  SRR4302535.1430381/1‑94 (MQ=60)

          CCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAG                                                                                 >  SRR4302535.630698/1‑94 (MQ=60)

          CCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAG                                                                                 >  SRR4302535.677138/1‑94 (MQ=60)

           CTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGC                                                                                >  SRR4302535.2151421/1‑94 (MQ=60)

           CTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGC                                                                                >  SRR4302535.4284255/1‑94 (MQ=60)

            TCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCG                                                                               >  SRR4302535.1758595/1‑94 (MQ=60)

             CCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGG                                                                              <  SRR4302535.4309672/94‑1 (MQ=60)

               CCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTC                                                                            <  SRR4302535.4602211/94‑1 (MQ=60)

                CTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCC                                                                           <  SRR4302535.1527480/94‑1 (MQ=60)

                CTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCC                                                                           <  SRR4302535.3195835/94‑1 (MQ=60)

                CTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCC                                                                           <  SRR4302535.366406/94‑1 (MQ=60)

                 TTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCG                                                                          <  SRR4302535.2141809/94‑1 (MQ=60)

                 TTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCG                                                                          <  SRR4302535.162193/94‑1 (MQ=60)

                   CGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGC                                                                        >  SRR4302535.1487278/1‑94 (MQ=60)

                   CGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGC                                                                        >  SRR4302535.3021009/1‑94 (MQ=60)

                    GGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCT                                                                       <  SRR4302535.2496331/94‑1 (MQ=60)

                    GGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCT                                                                       <  SRR4302535.4214338/94‑1 (MQ=60)

                    GGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCT                                                                       >  SRR4302535.2475620/1‑94 (MQ=60)

                     GAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTT                                                                      >  SRR4302535.3345962/1‑94 (MQ=60)

                       AGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTC                                                                    <  SRR4302535.3980041/94‑1 (MQ=60)

                       AGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTC                                                                    >  SRR4302535.3946113/1‑94 (MQ=60)

                       AGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTC                                                                    <  SRR4302535.1607608/94‑1 (MQ=60)

                        GGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCA                                                                   <  SRR4302535.2452341/94‑1 (MQ=60)

                         GTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCAC                                                                  >  SRR4302535.3316960/1‑94 (MQ=60)

                         GTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCAC                                                                  <  SRR4302535.1127427/94‑1 (MQ=60)

                           GAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCC                                                                <  SRR4302535.873186/94‑1 (MQ=60)

                            AGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCC                                                               >  SRR4302535.3481182/1‑94 (MQ=60)

                              ATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAG                                                             <  SRR4302535.4309962/94‑1 (MQ=60)

                              ATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAG                                                             <  SRR4302535.1536015/94‑1 (MQ=60)

                                 AGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCT                                                          <  SRR4302535.1805345/94‑1 (MQ=60)

                                 AGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCT                                                          <  SRR4302535.1701490/94‑1 (MQ=60)

                                 AGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCT                                                          <  SRR4302535.677837/94‑1 (MQ=60)

                                 AGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCT                                                          >  SRR4302535.1285557/1‑94 (MQ=60)

                                  GTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTG                                                         <  SRR4302535.903083/94‑1 (MQ=60)

                                   TGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGC                                                        >  SRR4302535.1921344/1‑94 (MQ=60)

                                      CAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGA                                                     >  SRR4302535.2325725/1‑94 (MQ=60)

                                        GTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATT                                                   >  SRR4302535.3887361/1‑94 (MQ=60)

                                        GTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATT                                                   <  SRR4302535.104548/94‑1 (MQ=60)

                                         TAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTT                                                  <  SRR4302535.4138288/94‑1 (MQ=60)

                                         TAACGCCGCCGCCGGAGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTT                                                  >  SRR4302535.2041124/1‑94 (MQ=60)

                                          AACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTT                                                 >  SRR4302535.1141137/1‑94 (MQ=60)

                                          AACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTT                                                 >  SRR4302535.1566909/1‑94 (MQ=60)

                                           ACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTT                                                >  SRR4302535.297879/1‑94 (MQ=60)

                                            CGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTA                                               <  SRR4302535.2513999/94‑1 (MQ=60)

                                             GCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAA                                              <  SRR4302535.1036611/94‑1 (MQ=60)

                                              CCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAA                                             <  SRR4302535.2911672/94‑1 (MQ=60)

                                              CCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAA                                             >  SRR4302535.3094015/1‑94 (MQ=60)

                                               CGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAAC                                            >  SRR4302535.1015207/1‑94 (MQ=60)

                                               CGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAAC                                            <  SRR4302535.236789/94‑1 (MQ=60)

                                               CGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAAC                                            <  SRR4302535.1566510/94‑1 (MQ=60)

                                               CGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAAC                                            <  SRR4302535.1099427/94‑1 (MQ=60)

                                               CGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAAC                                            <  SRR4302535.1333909/94‑1 (MQ=60)

                                                 CCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGT                                          <  SRR4302535.109210/94‑1 (MQ=60)

                                                 CCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGT                                          >  SRR4302535.2498770/1‑94 (MQ=60)

                                                  CGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTC                                         <  SRR4302535.2678234/94‑1 (MQ=60)

                                                  CGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTC                                         <  SRR4302535.1258857/94‑1 (MQ=60)

                                                   GCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCC                                        <  SRR4302535.2848488/94‑1 (MQ=60)

                                                     CGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCC                                      >  SRR4302535.724157/1‑94 (MQ=60)

                                                      GGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCC                                     <  SRR4302535.806406/94‑1 (MQ=60)

                                                      GGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCC                                     <  SRR4302535.430385/94‑1 (MQ=60)

                                                      GGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCC                                     <  SRR4302535.430386/94‑1 (MQ=60)

                                                      GGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCC                                     >  SRR4302535.3188999/1‑94 (MQ=60)

                                                        TGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGC                                   >  SRR4302535.2484367/1‑94 (MQ=60)

                                                          TTACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCAC                                 <  SRR4302535.1012878/94‑1 (MQ=60)

                                                            ACCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACAT                               >  SRR4302535.4292356/1‑94 (MQ=60)

                                                             CCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATA                              <  SRR4302535.4670081/94‑1 (MQ=60)

                                                             CCAGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATA                              <  SRR4302535.4488242/94‑1 (MQ=60)

                                                              CAGCTTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATAC                             <  SRR4302535.1034638/94‑1 (MQ=60)

                                                               CGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACA                            <  SRR4302535.396602/94‑1 (MQ=60)

                                                               AGCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACA                            <  SRR4302535.1559155/94‑1 (MQ=60)

                                                                GCGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAG                           <  SRR4302535.4263804/94‑1 (MQ=60)

                                                                 CGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGC                          <  SRR4302535.1596794/94‑1 (MQ=60)

                                                                 CGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGC                          >  SRR4302535.4509360/1‑94 (MQ=60)

                                                                 CGTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGC                          <  SRR4302535.4333293/94‑1 (MQ=60)

                                                                  GTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCG                         <  SRR4302535.2614911/94‑1 (MQ=60)

                                                                  GTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCG                         <  SRR4302535.4337306/94‑1 (MQ=60)

                                                                  GTTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCG                         >  SRR4302535.683683/1‑94 (MQ=60)

                                                                   TTGCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCGG                        >  SRR4302535.4635587/1‑94 (MQ=60)

                                                                     GCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCGGCG                      >  SRR4302535.2706945/1‑94 (MQ=60)

                                                                     GCCAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCGGCG                      <  SRR4302535.4193801/94‑1 (MQ=60)

                                                                       CAGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAA                    <  SRR4302535.437427/94‑1 (MQ=60)

                                                                        AGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAAC                   <  SRR4302535.2569178/94‑1 (MQ=60)

                                                                        AGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAAC                   <  SRR4302535.3237819/94‑1 (MQ=60)

                                                                        AGTGCCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAAC                   <  SRR4302535.3929974/94‑1 (MQ=60)

                                                                        AGTGCCGCACCAACAGCACCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAAC                   <  SRR4302535.2067977/94‑1 (MQ=60)

                                                                            CCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAACGCAG               <  SRR4302535.2055543/94‑1 (MQ=60)

                                                                            CCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAACGCAG               <  SRR4302535.2318397/94‑1 (MQ=60)

                                                                            CCGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAACGCAG               <  SRR4302535.2216467/94‑1 (MQ=60)

                                                                             CGCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAACGCAGT              >  SRR4302535.3208556/1‑94 (MQ=60)

                                                                              GCAGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCGGCGACCGCAGTG             >  SRR4302535.2053416/1‑94 (MQ=60)

                                                                                AGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAACGCAGTGTG           <  SRR4302535.3569686/94‑1 (MQ=60)

                                                                                AGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAACGCAGTGTG           >  SRR4302535.733101/1‑94 (MQ=60)

                                                                                AGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAACGCAGTGTG           <  SRR4302535.3970842/94‑1 (MQ=60)

                                                                                AGCAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAACGCAGTGTG           >  SRR4302535.455953/1‑94 (MQ=60)

                                                                                  CAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAACGCAGTGTGAT         >  SRR4302535.3478807/1‑94 (MQ=60)

                                                                                  CAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAACGCAGTGTGAT         <  SRR4302535.3055495/94‑1 (MQ=60)

                                                                                  CAACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAACGCAGTGTGAT         <  SRR4302535.1913404/94‑1 (MQ=60)

                                                                                   AACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAACGCAGTGTGATA        <  SRR4302535.2116635/94‑1 (MQ=60)

                                                                                   AACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAACGCAGTGTGATA        >  SRR4302535.769907/1‑94 (MQ=60)

                                                                                    ACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAACGCAGTGTGATAA       <  SRR4302535.1336669/94‑1 (MQ=60)

                                                                                    ACAGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAACGCAGTGTGATAA       <  SRR4302535.2858244/94‑1 (MQ=60)

                                                                                      AGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAACGCAGTGTGATAATC     <  SRR4302535.3214754/94‑1 (MQ=60)

                                                                                      AGCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAACGCAGTGTGATAATC     >  SRR4302535.2724325/1‑94 (MQ=60)

                                                                                       GCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAACGCAGTGTGATAATCC    <  SRR4302535.648906/94‑1 (MQ=60)

                                                                                       GCGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAACGCAGTGTGATAATCC    <  SRR4302535.2506603/94‑1 (MQ=60)

                                                                                        CGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAACGCAGTGTGATAATCCG   <  SRR4302535.671114/94‑1 (MQ=60)

                                                                                        CGCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAACGCAGTGTGATAATCCG   <  SRR4302535.2114616/94‑1 (MQ=60)

                                                                                         GCCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAACGCAGTGTGATAATCCGA  >  SRR4302535.1903717/1‑94 (MQ=60)

CACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCACCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAACGCAGTGTGATAATCCGA  >  NC_000913/3673951‑3674133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`