Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,676,230	T→C	T197T (ACA→ACG)	yhjH ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,676,230	0	T	C	96.8%	826.4 / 8.2	279	T197T (ACA→ACG)	yhjH	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (7/2); new base C (141/129); total (148/131)
Fisher's exact test for biased strand distribution p-value = 1.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 292 total aligned reads displayed.

AACATCACGCCACTCTTCCGGCGTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGTGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTCACTTAGCGCAGAGAAATT  >  NC_000913/3676140‑3676322

aaCATCACGCCCCTCTTCCGGCGTTTCTACGCCCTCGACAATCGCCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAgcgt                                                                                            <  2:1260663/93‑1 (MQ=255)

      ccGCCACTCTTCCGGCGTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTcc                                                                                      <  1:4288654/92‑1 (MQ=255)

      aCGCCACTCTTCCGGCGTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTcc                                                                                      >  1:1190570/1‑93 (MQ=255)

      aCGCCACTCTTCCGGCGTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTcc                                                                                      >  2:3684532/1‑93 (MQ=255)

       cGCCACTCTTCCGGCGTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCt                                                                                     >  1:999055/1‑93 (MQ=255)

        gCCACTCTTCCGGCGTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCtt                                                                                    <  1:323642/93‑1 (MQ=255)

          cACTCTTCCGGCGTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTcc                                                                                  <  2:3830416/93‑1 (MQ=255)

           aCTCTTCCGGCGTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCg                                                                                 <  2:3665022/93‑1 (MQ=255)

            ctctTCCGGCGTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCgg                                                                                <  1:4136194/93‑1 (MQ=255)

               ttCCGGCGTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGa                                                                             >  1:1451592/1‑93 (MQ=255)

               ttCCGGAGTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCATGCGTCCTTCCGGcca                                                                             >  2:3617766/1‑91 (MQ=255)

                tCCGGCGTTGCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGAc                                                                            <  1:1513918/93‑1 (MQ=255)

                 ccGGCGTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACt                                                                           >  2:1536807/1‑93 (MQ=255)

                  cGGCGTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTg                                                                          <  1:1728623/93‑1 (MQ=255)

                    gCGTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGAc                                                                        >  1:3106465/1‑93 (MQ=255)

                    gCGTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGAc                                                                        >  2:522485/1‑93 (MQ=255)

                      gTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACgc                                                                      >  1:3964271/1‑93 (MQ=255)

                      gTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACgc                                                                      <  1:2110690/93‑1 (MQ=255)

                      gTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACgc                                                                      >  1:4376644/1‑93 (MQ=255)

                       tttCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACgca                                                                     >  2:3102724/1‑93 (MQ=255)

                       tttCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACgca                                                                     >  1:2186303/1‑93 (MQ=255)

                       tttCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACgca                                                                     >  1:939055/1‑93 (MQ=255)

                       tttCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACgca                                                                     <  2:4054016/93‑1 (MQ=255)

                        ttCTCCGCCCTCTACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACgcag                                                                    <  1:1063444/93‑1 (MQ=255)

                         tCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACgcagc                                                                   <  2:3392596/93‑1 (MQ=255)

                           tACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCAt                                                                 <  1:1298218/93‑1 (MQ=255)

                            aCGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATc                                                                >  1:4677725/1‑93 (MQ=255)

                            aCGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATc                                                                <  1:1557258/93‑1 (MQ=255)

                              gCCCTCGACAACCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATcac                                                              <  2:2818042/93‑1 (MQ=255)

                                ccTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACaa                                                            <  2:157777/93‑1 (MQ=255)

                                 cTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACaaa                                                           <  1:2633440/93‑1 (MQ=255)

                                 cTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACaaa                                                           <  2:1812113/93‑1 (MQ=255)

                                   cGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACa                                                         <  2:601928/93‑1 (MQ=255)

                                      cAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGtt                                                      >  2:144260/1‑93 (MQ=255)

                                      cAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGtt                                                      <  2:2880780/93‑1 (MQ=255)

                                       aaTCCCCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTtc                                                     <  2:2433498/93‑1 (MQ=255)

                                       aaTCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTtc                                                     >  1:2844779/1‑93 (MQ=255)

                                        aTCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGTGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTtct                                                    >  2:4412622/1‑93 (MQ=255)

                                        aTCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTtct                                                    <  2:3408583/93‑1 (MQ=255)

                                         tCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTtctc                                                   <  1:3687741/93‑1 (MQ=255)

                                           aCCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTcgc                                                 >  2:4239148/1‑93 (MQ=255)

                                            ccccGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTcgcg                                                >  2:719522/1‑93 (MQ=255)

                                             cccGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTcgcgc                                               <  2:690170/93‑1 (MQ=255)

                                              ccGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTcgcgcg                                              >  2:302664/1‑93 (MQ=255)

                                              ccGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTcgcgcg                                              >  2:1836264/1‑93 (MQ=255)

                                                gcgACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGAt                                            <  2:2348908/93‑1 (MQ=255)

                                                  gACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGAttt                                          <  1:590270/93‑1 (MQ=255)

                                                  gACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGAttt                                          >  2:3444097/1‑93 (MQ=255)

                                                   gcAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGAtttt                                         <  1:3550866/92‑1 (MQ=255)

                                                    cAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGTGTGTGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTg                                        >  1:2516927/1‑93 (MQ=255)

                                                    cAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTg                                        <  1:1568212/93‑1 (MQ=255)

                                                     aaTAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGa                                       <  1:760525/93‑1 (MQ=255)

                                                      aTAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGAt                                      >  1:3439675/1‑93 (MQ=255)

                                                       tAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATg                                     >  1:4066644/1‑93 (MQ=255)

                                                       tAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATg                                     <  1:2443044/93‑1 (MQ=255)

                                                       tAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATg                                     >  2:4516915/1‑93 (MQ=255)

                                                        aGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGt                                    >  2:3183928/1‑93 (MQ=255)

                                                        aGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGt                                    <  2:63901/93‑1 (MQ=255)

                                                        aGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGt                                    <  1:1245682/93‑1 (MQ=255)

                                                          cGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAg                                  <  2:752506/93‑1 (MQ=255)

                                                          cGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAg                                  <  2:1297624/93‑1 (MQ=255)

                                                             ttCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCa                               <  2:852114/93‑1 (MQ=255)

                                                             ttCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCa                               >  2:4154234/1‑93 (MQ=255)

                                                             ttCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGACGTAGTCa                               >  1:1924425/1‑93 (MQ=255)

                                                               cATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATa                             <  1:1952535/93‑1 (MQ=255)

                                                               cATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATa                             >  2:1694692/1‑93 (MQ=255)

                                                                cttAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATaa                            <  2:3708877/92‑1 (MQ=255)

                                                                aTTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATaa                            <  1:4421347/93‑1 (MQ=255)

                                                                 ttAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAAc                           <  2:2006053/93‑1 (MQ=255)

                                                                 ttAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAAc                           <  1:2346811/93‑1 (MQ=255)

                                                                 ttAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAAc                           <  2:4211111/93‑1 (MQ=255)

                                                                 ttAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAAc                           >  2:2604830/1‑93 (MQ=255)

                                                                 ttAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAAc                           >  2:1328859/1‑93 (MQ=255)

                                                                 ttAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAAc                           >  2:3961113/1‑93 (MQ=255)

                                                                 ttAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAAc                           >  1:4250214/1‑93 (MQ=255)

                                                                 ttAGATGTAAAAGCTAAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAAc                           <  2:2147936/93‑1 (MQ=255)

                                                                  tAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACg                          >  2:2552751/1‑93 (MQ=255)

                                                                   aGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGc                         >  2:1753967/1‑93 (MQ=255)

                                                                    gATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGca                        >  2:3285988/1‑93 (MQ=255)

                                                                    gATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGca                        >  2:1171594/1‑93 (MQ=255)

                                                                     aTGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGcac                       <  1:3309864/93‑1 (MQ=255)

                                                                      tgaaaaaGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGcact                      >  1:2010901/4‑93 (MQ=255)

                                                                      tGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGcact                      >  2:1964591/1‑93 (MQ=255)

                                                                      tGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGcact                      >  1:2936135/1‑93 (MQ=255)

                                                                       gTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGcactt                     <  1:871243/93‑1 (MQ=255)

                                                                        tAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGcacttc                    <  1:2224967/93‑1 (MQ=255)

                                                                        tAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGcacttc                    <  2:3989760/93‑1 (MQ=255)

                                                                        tAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGcacttc                    <  1:3172968/93‑1 (MQ=255)

                                                                         aaaaGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGcacttca                   >  1:4352816/1‑93 (MQ=255)

                                                                         aaaaGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGcacttca                   <  1:1854031/93‑1 (MQ=255)

                                                                          aaaGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGcacttcac                  <  2:1791609/93‑1 (MQ=255)

                                                                             gCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTCACTTa               <  1:1032970/93‑1 (MQ=255)

                                                                             gCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTCACTTa               <  2:3500301/93‑1 (MQ=255)

                                                                               tGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTCACTTAgc             >  2:472083/1‑93 (MQ=255)

                                                                                  gaAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTCACTTAGCGCa          >  2:2298335/1‑93 (MQ=255)

                                                                                    aaaGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTCACTTAGCGCaga        <  2:2459626/93‑1 (MQ=255)

                                                                                     aaGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTCACTTAGCGCagag       <  1:2236652/93‑1 (MQ=255)

                                                                                      agagTGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTCACTTAGCGCAgaga      >  1:4158245/1‑93 (MQ=255)

                                                                                       gagCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTCACTTAGCGCAGAGaa     >  1:2665951/1‑93 (MQ=255)

                                                                                       gagCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTCACTTAGCGCAGAGaa     >  1:3800104/1‑93 (MQ=255)

AACATCACGCCACTCTTCCGGCGTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGTGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTCACTTAGCGCAGAGAAATT  >  NC_000913/3676140‑3676322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTGAACATCACGCCACTCTTCCGGCGTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGTGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTCACTTAGCGCAGAGAAATTT  >  NC_000913/3676137‑3676323

CTGAACATCACGCCACTCTTCCGGCGTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGC                                                                                               <  SRR4302535.288422/94‑1 (MQ=60)

CTGAACATCACGCCACTCTTCCGGCGTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGC                                                                                               <  SRR4302535.1928137/94‑1 (MQ=60)

 TGAACATCACGCCACTCTTCCGGCGTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCG                                                                                              <  SRR4302535.981303/94‑1 (MQ=60)

 TGAACATCACGCCACTCTTCCGGCGTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCG                                                                                              <  SRR4302535.208911/94‑1 (MQ=60)

 TGAACATCACGCCACTCTTCCGGCGTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCG                                                                                              <  SRR4302535.834946/94‑1 (MQ=60)

   AACATCACGCCACTCTTCCGGCGTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTG                                                                                            >  SRR4302535.3403166/1‑94 (MQ=60)

    ACATCACGCCACTCTTCCGGCGTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGC                                                                                           >  SRR4302535.311602/1‑94 (MQ=60)

     CATCACGCCACTCTTCCGGCGTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCG                                                                                          <  SRR4302535.1299053/94‑1 (MQ=60)

     CATCACGCCACTCTTCCGGCGTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCG                                                                                          >  SRR4302535.1534182/1‑94 (MQ=60)

      ATCACGCCACTCTTCCGGCGTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGT                                                                                         >  SRR4302535.1946859/1‑94 (MQ=60)

      ATCACGCCACTCTTCCGGCGTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGT                                                                                         >  SRR4302535.874047/1‑94 (MQ=60)

        CACGCCCCTCTTCCGGCGTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCC                                                                                       <  SRR4302535.843346/94‑1 (MQ=60)

        CACGCCACTCTTCCGGCGTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCC                                                                                       >  SRR4302535.1199094/1‑94 (MQ=60)

         CCGCCACTCTTCCGGCGTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCT                                                                                      <  SRR4302535.4317991/94‑1 (MQ=60)

         ACGCCACTCTTCCGGCGTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCT                                                                                      >  SRR4302535.1006141/1‑94 (MQ=60)

           GCCACTCTTCCGGCGTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTC                                                                                    <  SRR4302535.325877/94‑1 (MQ=60)

           GCCACTCTTCCGGCGTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTC                                                                                    >  SRR4302535.2304467/1‑94 (MQ=60)

               CTCTTCCGGCGTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGC                                                                                <  SRR4302535.4164514/94‑1 (MQ=60)

                CCTTCCGGCGTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCG                                                                               <  SRR4302535.1099766/94‑1 (MQ=60)

                 CTTCCGGCGTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGA                                                                              >  SRR4302535.1461973/1‑94 (MQ=60)

                   TCCGGCGTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACT                                                                            <  SRR4302535.3016456/94‑1 (MQ=60)

                   TCCGGCGTTGCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACT                                                                            <  SRR4302535.1524823/94‑1 (MQ=60)

                     CGGCGTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGA                                                                          <  SRR4302535.1741115/94‑1 (MQ=60)

                      GGCGTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGAC                                                                         >  SRR4302535.3128092/1‑94 (MQ=60)

                        CGTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGC                                                                       >  SRR4302535.1450972/1‑94 (MQ=60)

                        CGTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGC                                                                       >  SRR4302535.4406590/1‑94 (MQ=60)

                        CGTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGC                                                                       >  SRR4302535.3991351/1‑94 (MQ=60)

                         GTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCA                                                                      <  SRR4302535.2125915/94‑1 (MQ=60)

                         GTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCA                                                                      >  SRR4302535.2201883/1‑94 (MQ=60)

                         GTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCA                                                                      >  SRR4302535.945707/1‑94 (MQ=60)

                          TTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAG                                                                     <  SRR4302535.1341146/94‑1 (MQ=60)

                           TTCTCCGCCCTCTACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGC                                                                    <  SRR4302535.1071012/94‑1 (MQ=60)

                           TTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGGAGC                                                                    >  SRR4302535.4314940/1‑94 (MQ=60)

                           TTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGC                                                                    >  SRR4302535.3218902/1‑94 (MQ=60)

                           TTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGC                                                                    >  SRR4302535.41943/1‑94 (MQ=60)

                           TTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGC                                                                    <  SRR4302535.4610360/94‑1 (MQ=60)

                            TCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCA                                                                   <  SRR4302535.2480613/94‑1 (MQ=60)

                             CTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCAT                                                                  <  SRR4302535.3812289/94‑1 (MQ=60)

                              TACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATC                                                                 <  SRR4302535.1307477/94‑1 (MQ=60)

                              TACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATC                                                                 >  SRR4302535.4709291/1‑94 (MQ=60)

                               ACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCA                                                                <  SRR4302535.1568485/94‑1 (MQ=60)

                                 GCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACA                                                              >  SRR4302535.1559938/1‑94 (MQ=60)

                                  CCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAA                                                             >  SRR4302535.953847/1‑94 (MQ=60)

                                    CTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAAC                                                           <  SRR4302535.3261399/94‑1 (MQ=60)

                                    CTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAAC                                                           <  SRR4302535.2652311/94‑1 (MQ=60)

                                    CTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAAC                                                           <  SRR4302535.4398484/94‑1 (MQ=60)

                                      CGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAG                                                         >  SRR4302535.1577230/1‑94 (MQ=60)

                                      CGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAG                                                         <  SRR4302535.2353037/94‑1 (MQ=60)

                                       CACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGTGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGT                                                        <  SRR4302535.1674987/94‑1 (MQ=60)

                                        ACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGTGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTT                                                       <  SRR4302535.4171027/94‑1 (MQ=60)

                                        ACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTT                                                       <  SRR4302535.3839700/94‑1 (MQ=60)

                                        ACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTT                                                       >  SRR4302535.347040/1‑94 (MQ=60)

                                        ACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTT                                                       <  SRR4302535.2680607/94‑1 (MQ=60)

                                         CAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTC                                                      >  SRR4302535.2864946/1‑94 (MQ=60)

                                         CAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTC                                                      >  SRR4302535.1958513/1‑94 (MQ=60)

                                         CAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTC                                                      <  SRR4302535.2752136/94‑1 (MQ=60)

                                          CATCACCCCGCGACAATAGCGATTCATTCGATGTAACCGCTGAGCAACGCGCGTGCGTCCTTCCGGCGACTGCCGCAGCATCACAACCAGTTCT                                                     <  SRR4302535.4714259/94‑1 (MQ=50)

                                          AATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCT                                                     >  SRR4302535.1219805/1‑94 (MQ=60)

                                            TCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCG                                                   <  SRR4302535.3713256/94‑1 (MQ=60)

                                            TCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCG                                                   <  SRR4302535.2016149/94‑1 (MQ=60)

                                             CACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGC                                                  <  SRR4302535.1056541/94‑1 (MQ=60)

                                               CCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGC                                                >  SRR4302535.898665/1‑94 (MQ=60)

                                                CCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCCGCATCACAAACAGTTCTCGCGCG                                               >  SRR4302535.3717997/1‑94 (MQ=60)

                                                CCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCG                                               <  SRR4302535.1903689/94‑1 (MQ=60)

                                                  CGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCATGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGAT                                             >  SRR4302535.4345407/1‑94 (MQ=60)

                                                   GCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATT                                            <  SRR4302535.1304199/94‑1 (MQ=60)

                                                   GCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATT                                            <  SRR4302535.1514467/94‑1 (MQ=60)

                                                   GCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATT                                            <  SRR4302535.2190679/94‑1 (MQ=60)

                                                    CGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTT                                           >  SRR4302535.1174033/1‑94 (MQ=60)

                                                     GACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTT                                          >  SRR4302535.3617335/1‑94 (MQ=60)

                                                     GACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTT                                          >  SRR4302535.1858802/1‑94 (MQ=60)

                                                     GACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTT                                          <  SRR4302535.685837/94‑1 (MQ=60)

                                                     GACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTT                                          <  SRR4302535.594405/94‑1 (MQ=60)

                                                      GCAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTG                                         <  SRR4302535.3575507/94‑1 (MQ=60)

                                                      ACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGTGTGTGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTG                                         >  SRR4302535.2535070/1‑94 (MQ=60)

                                                       CAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGA                                        >  SRR4302535.2079902/1‑94 (MQ=60)

                                                       CAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGA                                        <  SRR4302535.1579529/94‑1 (MQ=60)

                                                       CAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGA                                        >  SRR4302535.1636120/1‑94 (MQ=60)

                                                       CAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGA                                        <  SRR4302535.1862711/94‑1 (MQ=60)

                                                       CAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGA                                        >  SRR4302535.2374350/1‑94 (MQ=60)

                                                        AATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGAT                                       >  SRR4302535.267265/1‑94 (MQ=60)

                                                        AATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGAT                                       <  SRR4302535.765884/94‑1 (MQ=60)

                                                        AATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGAT                                       >  SRR4302535.3463594/1‑94 (MQ=60)

                                                        AATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCAGGCGACTGACTCAGCATCACAAACAGTTCTCGCGCGATTTTGAT                                       >  SRR4302535.4426933/1‑94 (MQ=60)

                                                         ATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATG                                      >  SRR4302535.4094471/1‑94 (MQ=60)

                                                         ATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATG                                      <  SRR4302535.3382179/94‑1 (MQ=60)

                                                          TAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGT                                     <  SRR4302535.2460644/94‑1 (MQ=60)

                                                           AGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTA                                    <  SRR4302535.4116226/94‑1 (MQ=60)

                                                           AGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTA                                    >  SRR4302535.2956260/1‑94 (MQ=60)

                                                           AGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTA                                    <  SRR4302535.1254571/94‑1 (MQ=60)

                                                            GCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAG                                   <  SRR4302535.4037468/94‑1 (MQ=60)

                                                            GCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAG                                   <  SRR4302535.4030637/94‑1 (MQ=60)

                                                            GCGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAG                                   <  SRR4302535.3634355/94‑1 (MQ=60)

                                                             CGATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGT                                  >  SRR4302535.1901855/1‑94 (MQ=60)

                                                              GATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTC                                 >  SRR4302535.4241579/1‑94 (MQ=60)

                                                              GATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGCCCTTCCGGCGACTGACGCAGTATCACAAACAGTTCTCCAGCGATTTTGATGTAGTA                                 >  SRR4302535.879792/1‑94 (MQ=60)

                                                               ATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCA                                >  SRR4302535.291955/1‑94 (MQ=60)

                                                               ATTCATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGACGTAGTCA                                >  SRR4302535.1938295/1‑94 (MQ=60)

                                                                TTCATTAGATGTAAAACCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCAT                               <  SRR4302535.2440734/94‑1 (MQ=60)

                                                                  CATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAA                             <  SRR4302535.4382920/94‑1 (MQ=60)

                                                                  CATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAA                             <  SRR4302535.1966589/94‑1 (MQ=60)

                                                                   ATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAAC                            >  SRR4302535.4279326/1‑94 (MQ=60)

                                                                   ATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAAC                            <  SRR4302535.1478902/94‑1 (MQ=60)

                                                                   ATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAAC                            <  SRR4302535.4451624/94‑1 (MQ=60)

                                                                   ATTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAAC                            >  SRR4302535.2513840/1‑94 (MQ=60)

                                                                    TTAGATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACG                           <  SRR4302535.2363633/94‑1 (MQ=60)

                                                                       GATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCAC                        >  SRR4302535.3889166/1‑94 (MQ=60)

                                                                       GATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCAC                        <  SRR4302535.2163107/94‑1 (MQ=60)

                                                                        ATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACT                       <  SRR4302535.3332894/94‑1 (MQ=60)

                                                                        ATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACT                       >  SRR4302535.680750/1‑94 (MQ=60)

                                                                        ATGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACT                       >  SRR4302535.2956795/1‑94 (MQ=60)

                                                                        ATGAAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACT                       >  SRR4302535.2025377/1‑94 (MQ=60)

                                                                         TGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTT                      <  SRR4302535.1202149/94‑1 (MQ=60)

                                                                         TGTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTT                      >  SRR4302535.313924/1‑94 (MQ=60)

                                                                          GTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTC                     >  SRR4302535.4170510/1‑94 (MQ=60)

                                                                          GTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTC                     <  SRR4302535.877417/94‑1 (MQ=60)

                                                                          GTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTC                     >  SRR4302535.447174/1‑94 (MQ=60)

                                                                          GTAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTC                     >  SRR4302535.2243095/1‑94 (MQ=60)

                                                                           TAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTCA                    <  SRR4302535.3194933/94‑1 (MQ=60)

                                                                           TAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTCA                    <  SRR4302535.2240799/94‑1 (MQ=60)

                                                                           TAAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTCA                    >  SRR4302535.4382606/1‑94 (MQ=60)

                                                                            AAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTCAC                   >  SRR4302535.2777301/1‑94 (MQ=60)

                                                                            AAAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTCAC                   <  SRR4302535.1867386/94‑1 (MQ=60)

                                                                             AAAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTCACT                  <  SRR4302535.2588170/94‑1 (MQ=60)

                                                                              AAGCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTCACTT                 <  SRR4302535.3525721/94‑1 (MQ=60)

                                                                                GCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTCACTTAG               <  SRR4302535.1040309/94‑1 (MQ=60)

                                                                                GCTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTCACTTAG               <  SRR4302535.2111368/94‑1 (MQ=60)

                                                                                 CTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTCACTTAGC              >  SRR4302535.955190/1‑94 (MQ=60)

                                                                                 CTGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTCACTTAGC              >  SRR4302535.3226592/1‑94 (MQ=60)

                                                                                  TGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCATCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTCACTTAGCG             <  SRR4302535.519171/94‑1 (MQ=60)

                                                                                  TGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTCACTTAGCG             <  SRR4302535.2244385/94‑1 (MQ=60)

                                                                                  TGAGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTCACTTAGCG             >  SRR4302535.2640691/1‑94 (MQ=60)

                                                                                    AGAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTCACTTAGCGCA           >  SRR4302535.4429011/1‑94 (MQ=60)

                                                                                     GAAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTCACTTAGCGCAG          >  SRR4302535.1569094/1‑94 (MQ=60)

                                                                                      AAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAAGAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTCACTTAGCGCAGA         >  SRR4302535.3323494/1‑94 (MQ=60)

                                                                                      AAAAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTCACTTAGCGCAGA         >  SRR4302535.2206931/1‑94 (MQ=60)

                                                                                        AAGAGTGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTCACTTAGCGCAGAGA       >  SRR4302535.4186710/1‑94 (MQ=60)

                                                                                        AAGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTCACTTAGCGCAGAGA       <  SRR4302535.2252564/94‑1 (MQ=60)

                                                                                         AGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTCACTTAGCGCAGAGAA      >  SRR4302535.4319604/1‑94 (MQ=60)

                                                                                         AGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTCACTTAGCGCAGAGAA      <  SRR4302535.4158199/94‑1 (MQ=60)

                                                                                         AGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTCACTTAGCGCAGAGAA      >  SRR4302535.2685037/1‑94 (MQ=60)

                                                                                         AGAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTCACTTAGCGCAGAGAA      >  SRR4302535.3826299/1‑94 (MQ=60)

                                                                                          GAGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTCACTTAGCGCAGAGAAA     >  SRR4302535.4182490/1‑94 (MQ=60)

                                                                                           AGCGTGCTTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTCACTTAGCGCAGAGAAAT    <  SRR4302535.385891/94‑1 (MQ=60)

                                                                                           AGCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTCACTTAGCGCAGAGAAAT    <  SRR4302535.2891128/94‑1 (MQ=60)

                                                                                            GCGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAAGGCACTTCACTTAGCGCAGAGAAATT   >  SRR4302535.977021/1‑94 (MQ=60)

                                                                                             TGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTCACTTAGCGCAGAGAAATTT  <  SRR4302535.2205916/94‑1 (MQ=60)

                                                                                             CGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTCACTTAGCGCAGAGAAATTT  >  SRR4302535.4043041/1‑94 (MQ=60)

CTGAACATCACGCCACTCTTCCGGCGTTTCTACGCCCTCGACAATCACCCCGCGACAATAGCGATTCATTAGATGTAAAAGCTGAGAAAAGAGTGTGCGTCCTTCCGGCGACTGACGCAGCATCACAAACAGTTCTCGCGCGATTTTGATGTAGTCATAACGCACTTCACTTAGCGCAGAGAAATTT  >  NC_000913/3676137‑3676323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`