Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,686,775	+T	intergenic (‑23/+9)	b3530 ← / ← yhjM	putative oxidoreductase subunit/putative endoglucanase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,686,775	1	.	T	99.6%	905.4 / ‑4.4	251	intergenic (‑23/+9)	b3530/yhjM	putative oxidoreductase subunit/putative endoglucanase
Reads supporting (aligned to +/- strand): ref base . (1/0); new base T (116/134); total (117/134)
Fisher's exact test for biased strand distribution p-value = 4.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 277 total aligned reads displayed.

CGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑G‑GAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATC  >  NC_000913/3686684‑3686861

 gCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑gtg                                                                                       >  2:1101196/1‑91 (MQ=255)

 gCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCACACCGAGGGAAAGC‑gtg                                                                                       >  1:1800236/1‑91 (MQ=255)

  cTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGGAAGC‑gtga                                                                                      >  2:632858/1‑90 (MQ=255)

   ttCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑gtgaa                                                                                     >  1:1194735/1‑89 (MQ=255)

   ttCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑gtgaa                                                                                     <  2:4116282/93‑5 (MQ=255)

   ttCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑gtgaa                                                                                     >  1:2089642/1‑89 (MQ=255)

   ttCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGCGAAAGC‑gtgaa                                                                                     >  1:4306536/1‑89 (MQ=255)

      gCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAatat                                                                                  >  2:3369500/1‑93 (MQ=255)

       ccTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATg                                                                                 <  1:4393059/93‑1 (MQ=255)

       ccTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATg                                                                                 <  2:3908922/93‑1 (MQ=255)

        cTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGt                                                                                <  1:3840460/93‑1 (MQ=255)

        cTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGt                                                                                >  1:3755859/1‑93 (MQ=255)

        cTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGt                                                                                >  1:3692645/1‑93 (MQ=255)

         tAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGtt                                                                               <  2:3325467/93‑1 (MQ=255)

         tAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGtt                                                                               >  1:3079061/1‑93 (MQ=255)

         tAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGtt                                                                               >  2:2320631/1‑93 (MQ=255)

         tAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGtt                                                                               >  2:4558047/1‑93 (MQ=255)

         tAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGtt                                                                               >  1:3575610/1‑93 (MQ=255)

          aaCCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGttt                                                                              <  2:4376840/93‑1 (MQ=255)

          aaCCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGttt                                                                              >  2:2351650/1‑93 (MQ=255)

          aaCCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGttt                                                                              >  2:3729578/1‑93 (MQ=255)

          aaCCGAACTGGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGttt                                                                              <  2:3442880/93‑1 (MQ=255)

           aCCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTa                                                                             <  1:4599340/93‑1 (MQ=255)

             cGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAgt                                                                           >  2:2412423/1‑93 (MQ=255)

                aCTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGtgtg                                                                        >  1:548134/1‑93 (MQ=255)

                 cTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGa                                                                       >  2:2914038/1‑93 (MQ=255)

                 cTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGa                                                                       <  2:3295861/93‑1 (MQ=255)

                 cTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGa                                                                       <  2:2683859/93‑1 (MQ=255)

                 cTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGa                                                                       >  1:2769493/1‑93 (MQ=255)

                    ggtCCAGCAAGTGTTGCTGGGGGGTTTGTGCTGCCTCGACCCTCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAAtt                                                                    <  1:3831239/91‑1 (MQ=255)

                    gCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAAtt                                                                    <  2:2664929/93‑1 (MQ=255)

                      tCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTg                                                                  >  1:3011350/1‑93 (MQ=255)

                       ccAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTgc                                                                 <  1:394774/93‑1 (MQ=255)

                       ccAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTgc                                                                 <  1:527035/93‑1 (MQ=255)

                       ccAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTgc                                                                 <  2:2221426/93‑1 (MQ=255)

                       ccAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTgc                                                                 <  2:4147664/93‑1 (MQ=255)

                           cAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCAt                                                             >  2:143713/1‑93 (MQ=255)

                           cAACTGTTCCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCAt                                                             <  1:959974/93‑1 (MQ=255)

                            aaCTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCAtt                                                            >  2:861011/1‑93 (MQ=255)

                            aaCTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCAtt                                                            <  1:3624892/93‑1 (MQ=255)

                             aCTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTc                                                           >  1:2632215/1‑93 (MQ=255)

                             aCTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTc                                                           <  1:2017703/93‑1 (MQ=255)

                               tGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCt                                                         <  1:1468644/93‑1 (MQ=255)

                                   gCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGcc                                                     <  1:1303166/93‑1 (MQ=255)

                                   gCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGcc                                                     <  2:1957378/93‑1 (MQ=255)

                                    gtGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTAAATATGTTTAGTGTGAATTTGCGCATTCCTGGccc                                                    <  1:448086/92‑1 (MQ=255)

                                     tGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGcccc                                                   >  1:226737/1‑93 (MQ=255)

                                      gAGCGGTTGTTGCTTCCTTGACCATCGGCATGACGGCCAGACCGAGTGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCa                                                  <  2:3234613/93‑1 (MQ=255)

                                        gCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGt                                                >  1:4013504/1‑93 (MQ=255)

                                        gCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGt                                                >  2:830261/1‑93 (MQ=255)

                                         cGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTc                                               <  1:1563064/93‑1 (MQ=255)

                                           gTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAg                                             >  1:2306152/1‑93 (MQ=255)

                                              ggTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGta                                          <  1:1675633/93‑1 (MQ=255)

                                               gTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGtaa                                         <  1:1531372/93‑1 (MQ=255)

                                                   tgcCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAAc                                     <  2:1351403/93‑1 (MQ=255)

                                                   tgacTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAAc                                     <  1:4256541/90‑1 (MQ=255)

                                                    gcCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACt                                    <  1:2527071/93‑1 (MQ=255)

                                                         gACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCt                               <  2:1257215/93‑1 (MQ=255)

                                                         gACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCt                               >  1:3387814/1‑93 (MQ=255)

                                                         gACCATCGGCATGACCGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGCCAGGTAATAACTCACCt                               >  2:2139785/1‑93 (MQ=255)

                                                           ccATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCttt                             >  2:50768/1‑93 (MQ=255)

                                                           ccATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCttt                             <  1:1063105/93‑1 (MQ=255)

                                                           ccATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCttt                             >  1:4095778/1‑93 (MQ=255)

                                                            cATCGGCATGACGGCCAGACCGAGGGAAAGCGG‑GAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCtttt                            >  2:4597555/1‑93 (MQ=255)

                                                             aTCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTg                           >  2:1430155/1‑93 (MQ=255)

                                                             aTCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTg                           >  1:2454642/1‑93 (MQ=255)

                                                              tCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGt                          <  2:1776066/93‑1 (MQ=255)

                                                              tCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGt                          >  2:1497691/1‑93 (MQ=255)

                                                              tCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGt                          >  2:1225815/1‑93 (MQ=255)

                                                              tCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGt                          >  2:3695763/1‑93 (MQ=255)

                                                               cGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTc                         <  2:1763597/93‑1 (MQ=255)

                                                               cGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTc                         <  2:3963346/93‑1 (MQ=255)

                                                                   aTGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCgaga                     >  1:823921/1‑93 (MQ=255)

                                                                    tGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGaa                    <  1:684339/93‑1 (MQ=255)

                                                                    tGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGaa                    <  2:428354/93‑1 (MQ=255)

                                                                     gACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAg                   <  1:1958727/93‑1 (MQ=255)

                                                                      aCGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGc                  >  2:2052694/1‑93 (MQ=255)

                                                                      aCGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGc                  <  2:2464491/93‑1 (MQ=255)

                                                                       cGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCg                 >  2:2594192/1‑93 (MQ=255)

                                                                       cGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCg                 <  1:1504417/93‑1 (MQ=255)

                                                                       cGGCCAGACCCCGGGGAAGC‑GCGAGAATTTTTTGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGGCATAACTCACCTTTTGTCGAGAAGCg                 >  2:3199086/1‑93 (MQ=255)

                                                                        ggCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCgg                >  1:4639910/1‑93 (MQ=255)

                                                                        ggCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCgg                >  2:536685/1‑93 (MQ=255)

                                                                          ccAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGaa              <  2:1002309/93‑1 (MQ=255)

                                                                           cAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGaaa             >  2:38315/1‑93 (MQ=255)

                                                                           cAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGaaa             <  1:3331035/93‑1 (MQ=255)

                                                                            aGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAAc            <  2:276295/93‑1 (MQ=255)

                                                                             gACCGGGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCGCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGCAACg           >  2:2959530/1‑93 (MQ=255)

                                                                             gACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACg           >  2:3126796/1‑93 (MQ=255)

                                                                             gACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACg           >  1:2497392/1‑93 (MQ=255)

                                                                               ccGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGgt         >  2:1809691/1‑93 (MQ=255)

                                                                                 gAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGgtgt       >  1:4111003/1‑93 (MQ=255)

                                                                                 gAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGgtgt       <  1:4500240/93‑1 (MQ=255)

                                                                                  aGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGtt      >  1:253450/1‑93 (MQ=255)

                                                                                  aGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGtt      >  1:3498975/1‑93 (MQ=255)

                                                                                  aGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGtt      >  2:3038353/1‑93 (MQ=255)

                                                                                   gggAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTg     >  2:904004/1‑93 (MQ=255)

                                                                                   gggAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTg     >  2:2792688/1‑93 (MQ=255)

                                                                                      aaaGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATc  >  1:1102027/1‑93 (MQ=255)

                                                                                      aaaGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGCAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGatcatc  >  2:4489349/1‑93 (MQ=255)

CGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑G‑GAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATC  >  NC_000913/3686684‑3686861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCG‑GAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCAGCCT  >  NC_000913/3686682‑3686868

GTCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCG                                                                                                <  SRR4302535.4178214/94‑1 (MQ=60)

GTCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCG                                                                                                <  SRR4302535.3756161/94‑1 (MQ=60)

 TCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCG‑G                                                                                              >  SRR4302535.460232/1‑94 (MQ=60)

  CGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGtg                                                                                              >  SRR4302535.353346/1‑92 (MQ=60)

  CGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGtg                                                                                              >  SRR4302535.4490119/1‑92 (MQ=60)

  CGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCACACCGAGGGAAAGCGtg                                                                                              >  SRR4302535.1813249/1‑92 (MQ=60)

   GCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGtga                                                                                             >  SRR4302535.467889/1‑91 (MQ=60)

   GCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGtga                                                                                             <  SRR4302535.4315607/94‑4 (MQ=60)

    CTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAA                                                                                            >  SRR4302535.1906387/1‑94 (MQ=60)

    CTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAA                                                                                            >  SRR4302535.1203293/1‑94 (MQ=60)

    CTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAA                                                                                            >  SRR4302535.2104699/1‑94 (MQ=60)

    CTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGCGAAAGCGTGAA                                                                                            >  SRR4302535.4336000/1‑94 (MQ=60)

     TTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAAT                                                                                           >  SRR4302535.3539660/1‑94 (MQ=60)

     TTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAAT                                                                                           >  SRR4302535.4648100/1‑94 (MQ=60)

     TTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCGGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAAT                                                                                           <  SRR4302535.4641677/94‑1 (MQ=60)

      TCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATA                                                                                          <  SRR4302535.2720753/94‑1 (MQ=60)

       CGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATAT                                                                                         >  SRR4302535.1499296/1‑94 (MQ=60)

       CGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATAT                                                                                         <  SRR4302535.4134025/94‑1 (MQ=60)

       CGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATAT                                                                                         <  SRR4302535.4086911/94‑1 (MQ=60)

        GCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTACTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATG                                                                                        >  SRR4302535.3330247/1‑94 (MQ=60)

         CCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGT                                                                                       >  SRR4302535.3718203/1‑94 (MQ=60)

         CCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGT                                                                                       <  SRR4302535.3727946/94‑1 (MQ=60)

         CCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGT                                                                                       <  SRR4302535.2485362/94‑1 (MQ=60)

         CCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGT                                                                                       >  SRR4302535.3781839/1‑94 (MQ=60)

         CCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGT                                                                                       <  SRR4302535.4423118/94‑1 (MQ=60)

         CCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATAGGAATGACGGCCAGACCGAGGGAAAGCGTGAATATGT                                                                                       >  SRR4302535.4200149/1‑94 (MQ=60)

         CCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCCACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGT                                                                                       <  SRR4302535.2533028/94‑1 (MQ=60)

          CTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTT                                                                                      <  SRR4302535.3866924/94‑1 (MQ=60)

          CTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTT                                                                                      >  SRR4302535.4021578/1‑94 (MQ=60)

          CTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTT                                                                                      >  SRR4302535.3781326/1‑94 (MQ=60)

          CTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTT                                                                                      >  SRR4302535.3100533/1‑94 (MQ=60)

          CTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTT                                                                                      >  SRR4302535.561839/1‑94 (MQ=60)

          CTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTT                                                                                      >  SRR4302535.3600399/1‑94 (MQ=60)

          CTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCCGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTT                                                                                      >  SRR4302535.2537051/1‑94 (MQ=60)

           TAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTT                                                                                     >  SRR4302535.4653364/1‑94 (MQ=60)

             ACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAG                                                                                   <  SRR4302535.161582/94‑1 (MQ=60)

             ACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAG                                                                                   <  SRR4302535.4630626/94‑1 (MQ=60)

             ACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAG                                                                                   <  SRR4302535.751562/94‑1 (MQ=60)

              CCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGT                                                                                  <  SRR4302535.4483939/94‑1 (MQ=60)

              CCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGT                                                                                  <  SRR4302535.2566657/94‑1 (MQ=60)

              CCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGT                                                                                  <  SRR4302535.1128224/94‑1 (MQ=60)

               CGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTG                                                                                 >  SRR4302535.3651757/1‑94 (MQ=60)

               CGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTG                                                                                 <  SRR4302535.975290/94‑1 (MQ=60)

               CGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTG                                                                                 <  SRR4302535.928837/94‑1 (MQ=60)

               CGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTG                                                                                 <  SRR4302535.127438/94‑1 (MQ=60)

                GAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGT                                                                                >  SRR4302535.201594/1‑94 (MQ=60)

                 AACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTG                                                                               <  SRR4302535.4108070/94‑1 (MQ=60)

                 AACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTG                                                                               >  SRR4302535.551961/1‑94 (MQ=60)

                  ACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGA                                                                              >  SRR4302535.2789214/1‑94 (MQ=60)

                    TTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAAT                                                                            >  SRR4302535.1652706/1‑94 (MQ=60)

                      GGTCCAGCAAGTGTTGCTGGGGGGTTTGTGCTGCCTCGACCCTCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTT                                                                          <  SRR4302535.3857659/94‑1 (MQ=60)

                      GCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTT                                                                          >  SRR4302535.2431034/1‑94 (MQ=60)

                      GCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTT                                                                          <  SRR4302535.3920753/94‑1 (MQ=60)

                       CTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTG                                                                         >  SRR4302535.3032447/1‑94 (MQ=60)

                         CCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCG                                                                       <  SRR4302535.530715/94‑1 (MQ=60)

                         CCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCG                                                                       <  SRR4302535.397529/94‑1 (MQ=60)

                         CCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCG                                                                       <  SRR4302535.3071918/94‑1 (MQ=60)

                          CAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGC                                                                      <  SRR4302535.4558955/94‑1 (MQ=60)

                             CAACTGTTCCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATT                                                                   <  SRR4302535.966789/94‑1 (MQ=60)

                              AACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTC                                                                  >  SRR4302535.2651079/1‑94 (MQ=60)

                              AACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTC                                                                  >  SRR4302535.2711195/1‑94 (MQ=60)

                              AACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTC                                                                  >  SRR4302535.2281671/1‑94 (MQ=60)

                              AACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTC                                                                  <  SRR4302535.3650016/94‑1 (MQ=60)

                               ACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCC                                                                 <  SRR4302535.2032239/94‑1 (MQ=60)

                               ACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCC                                                                 <  SRR4302535.4331452/94‑1 (MQ=60)

                                CTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCT                                                                >  SRR4302535.1169347/1‑94 (MQ=60)

                                CTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCT                                                                <  SRR4302535.356440/94‑1 (MQ=60)

                                CTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCT                                                                <  SRR4302535.2722413/94‑1 (MQ=60)

                                 TGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTG                                                               <  SRR4302535.1479166/94‑1 (MQ=60)

                                  GTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGG                                                              <  SRR4302535.2505186/94‑1 (MQ=60)

                                    TGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCC                                                            >  SRR4302535.64986/1‑94 (MQ=60)

                                     GCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCC                                                           <  SRR4302535.1312470/94‑1 (MQ=60)

                                      GTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTAAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCC                                                          <  SRR4302535.451224/94‑1 (MQ=60)

                                      CTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCC                                                          <  SRR4302535.3998226/94‑1 (MQ=60)

                                      CTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCC                                                          >  SRR4302535.228298/1‑94 (MQ=60)

                                        GAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAG                                                        <  SRR4302535.2606385/94‑1 (MQ=60)

                                        GAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAG                                                        <  SRR4302535.2912950/94‑1 (MQ=60)

                                         AGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGT                                                       >  SRR4302535.4040958/1‑94 (MQ=60)

                                           CGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCA                                                     <  SRR4302535.1574325/94‑1 (MQ=60)

                                           CGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCA                                                     >  SRR4302535.1701508/1‑94 (MQ=60)

                                            GGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAG                                                    >  SRR4302535.3441798/1‑94 (MQ=60)

                                            GGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAG                                                    >  SRR4302535.2322646/1‑94 (MQ=60)

                                            GGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAG                                                    >  SRR4302535.1399711/1‑94 (MQ=60)

                                             GTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGG                                                   >  SRR4302535.3661806/1‑94 (MQ=60)

                                             GTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGG                                                   <  SRR4302535.3865328/94‑1 (MQ=60)

                                             GTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGG                                                   <  SRR4302535.1550030/94‑1 (MQ=60)

                                              TTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGT                                                  <  SRR4302535.2044648/94‑1 (MQ=60)

                                               TGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTA                                                 <  SRR4302535.4529207/94‑1 (MQ=60)

                                               TGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTA                                                 <  SRR4302535.3359930/94‑1 (MQ=60)

                                                GGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAA                                                <  SRR4302535.1687766/94‑1 (MQ=60)

                                                 GTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAAT                                               <  SRR4302535.1542407/94‑1 (MQ=60)

                                                   GCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAA                                             <  SRR4302535.1569448/94‑1 (MQ=60)

                                                    CTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAAC                                            <  SRR4302535.1534702/94‑1 (MQ=60)

                                                     TGCCTCGACCATCGGCATGACGGCCAGACCGAGGGCAAGCGTGAATATGTTTAGTGGGAATTTGCGCATTCCTGGCGCCAGTCAGGTAATAACT                                           >  SRR4302535.4185555/1‑94 (MQ=60)

                                                     TGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACT                                           >  SRR4302535.3440139/1‑94 (MQ=60)

                                                     TGACTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACT                                           <  SRR4302535.4285704/94‑1 (MQ=60)

                                                      GCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTC                                          <  SRR4302535.2545274/94‑1 (MQ=60)

                                                       CCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCA                                         <  SRR4302535.418916/94‑1 (MQ=60)

                                                       CCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCA                                         <  SRR4302535.4346811/94‑1 (MQ=60)

                                                        CTCGACCATCGCCAGGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTGGCGCATTCCTGG‑CCCAGTCAGGTAATAACTCACC                                       <  SRR4302535.854715/94‑1 (MQ=60)

                                                          CGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCT                                      >  SRR4302535.3411384/1‑94 (MQ=60)

                                                            ACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTT                                    <  SRR4302535.1301574/94‑1 (MQ=60)

                                                            ACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTT                                    >  SRR4302535.4123810/1‑94 (MQ=60)

                                                             CCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTT                                   <  SRR4302535.1070672/94‑1 (MQ=60)

                                                             CCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTT                                   <  SRR4302535.2149789/94‑1 (MQ=60)

                                                              CATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTG                                  >  SRR4302535.3874387/1‑94 (MQ=60)

                                                              CATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTG                                  >  SRR4302535.2472337/1‑94 (MQ=60)

                                                               ATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGT                                 <  SRR4302535.3783267/94‑1 (MQ=60)

                                                               ATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGT                                 >  SRR4302535.2297790/1‑94 (MQ=60)

                                                               ATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGT                                 >  SRR4302535.1523613/1‑94 (MQ=60)

                                                                TCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTC                                <  SRR4302535.3372203/94‑1 (MQ=60)

                                                                 CGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCG                               <  SRR4302535.3801832/94‑1 (MQ=60)

                                                                 CGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCG                               <  SRR4302535.547442/94‑1 (MQ=60)

                                                                 CGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCG                               >  SRR4302535.2005606/1‑94 (MQ=60)

                                                                 CGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCG                               <  SRR4302535.1085705/94‑1 (MQ=60)

                                                                    CATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGA                            >  SRR4302535.829695/1‑94 (MQ=60)

                                                                     ATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAA                           <  SRR4302535.3647242/94‑1 (MQ=60)

                                                                      TGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAG                          <  SRR4302535.923218/94‑1 (MQ=60)

                                                                      TGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAG                          <  SRR4302535.689129/94‑1 (MQ=60)

                                                                       GACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGC                         <  SRR4302535.1972826/94‑1 (MQ=60)

                                                                         CGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGG                       <  SRR4302535.1515236/94‑1 (MQ=60)

                                                                         CGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGG                       <  SRR4302535.2869252/94‑1 (MQ=60)

                                                                         CGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGG                       >  SRR4302535.4671372/1‑94 (MQ=60)

                                                                          GGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGA                      <  SRR4302535.692625/94‑1 (MQ=60)

                                                                             CAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAAC                   <  SRR4302535.1477960/94‑1 (MQ=60)

                                                                             CAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAAC                   <  SRR4302535.3354223/94‑1 (MQ=60)

                                                                              AGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACG                  >  SRR4302535.2515409/1‑94 (MQ=60)

                                                                               GACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGG                 >  SRR4302535.4384529/1‑94 (MQ=60)

                                                                                ACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGT                <  SRR4302535.1953613/94‑1 (MQ=60)

                                                                                 CCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTG               <  SRR4302535.2041983/94‑1 (MQ=60)

                                                                                  CGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGT              >  SRR4302535.4139144/1‑94 (MQ=60)

                                                                                  CGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGT              >  SRR4302535.2389879/1‑94 (MQ=60)

                                                                                   GAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTT             <  SRR4302535.4531147/94‑1 (MQ=60)

                                                                                   GAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTT             >  SRR4302535.3523297/1‑94 (MQ=60)

                                                                                   GAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTT             <  SRR4302535.494171/94‑1 (MQ=60)

                                                                                   GAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTT             >  SRR4302535.255201/1‑94 (MQ=60)

                                                                                     GGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGA           >  SRR4302535.319777/1‑94 (MQ=60)

                                                                                       GAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATC         >  SRR4302535.2546144/1‑94 (MQ=60)

                                                                                       GAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATC         >  SRR4302535.1109902/1‑94 (MQ=60)

                                                                                        AAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCC        >  SRR4302535.938676/1‑94 (MQ=60)

                                                                                         AAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCC       <  SRR4302535.531751/94‑1 (MQ=60)

                                                                                         AAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCC       >  SRR4302535.1267297/1‑94 (MQ=60)

                                                                                         AAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCC       >  SRR4302535.814891/1‑94 (MQ=60)

                                                                                         AAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCC       >  SRR4302535.3613206/1‑94 (MQ=60)

                                                                                          AGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCA      >  SRR4302535.380929/1‑94 (MQ=60)

                                                                                          AGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCA      >  SRR4302535.3663381/1‑94 (MQ=60)

                                                                                          AGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCA      >  SRR4302535.4218112/1‑94 (MQ=60)

                                                                                           GCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCAG     <  SRR4302535.1583648/94‑1 (MQ=60)

                                                                                             T‑GAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCAGCCT  >  SRR4302535.2235566/1‑94 (MQ=60)

                                                                                             T‑GAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCAGCCT  >  SRR4302535.4268178/1‑94 (MQ=60)

                                                                                             T‑GAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCAGCCT  <  SRR4302535.1872506/94‑1 (MQ=60)

GTCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCG‑GAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCAGCCT  >  NC_000913/3686682‑3686868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`