Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,731,386	T→C	V13A (GTA→GCA)	xylH →	XylH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,731,386	0	T	C	100.0%	725.5 / NA	232	V13A (GTA→GCA)	xylH	XylH
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (120/112); total (120/112)

Only 100 of 240 total aligned reads displayed.

TCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCT  >  NC_000913/3731296‑3731475

tCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCAc                                                                                         >  2:2832386/1‑93 (MQ=255)

tCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCAc                                                                                         >  2:438816/1‑93 (MQ=255)

 cATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCAcc                                                                                        <  1:4215295/93‑1 (MQ=255)

   aaaCCTGACGCAGGAGGAGGTGATGGAAGCCGCATTAAGGAGCGAACAGCATGTCGAAAATCAAGCCGTCGGAAGTGAAATTGGCCGCACCGa                                                                                      <  2:3314336/92‑1 (MQ=255)

     aCCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGaaa                                                                                    >  2:1365409/1‑91 (MQ=255)

     aCCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACa                                                                                    <  1:3529588/93‑1 (MQ=255)

      ccTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACAt                                                                                   <  2:3091413/93‑1 (MQ=255)

       cTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATc                                                                                  >  2:1871177/1‑93 (MQ=255)

       cTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCAGTCTGAAGTGAAATTGGCCGAACCGACagc                                                                                  >  1:100766/1‑91 (MQ=255)

        tGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATcc                                                                                 >  1:1482044/1‑93 (MQ=255)

        tGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATcc                                                                                 <  2:3071126/93‑1 (MQ=255)

         gACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCg                                                                                <  1:2306491/93‑1 (MQ=255)

           cTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGt                                                                              <  1:587825/93‑1 (MQ=255)

             cAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTgg                                                                            >  1:2680959/1‑93 (MQ=255)

               ggAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCt                                                                          >  2:2025358/1‑93 (MQ=255)

                   cAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTtctc                                                                      <  1:2335136/93‑1 (MQ=255)

                     ggTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCg                                                                    >  2:881275/1‑93 (MQ=255)

                      gTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCgg                                                                   >  1:899858/1‑93 (MQ=255)

                         aTGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCt                                                                >  1:2485880/1‑93 (MQ=255)

                          tGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTg                                                               >  1:1509595/1‑93 (MQ=255)

                          tGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTg                                                               >  1:822912/1‑93 (MQ=255)

                            gAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGaa                                                             <  1:1624218/93‑1 (MQ=255)

                              aGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAAt                                                           >  1:4466431/1‑93 (MQ=255)

                               gCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATc                                                          <  1:2597293/93‑1 (MQ=255)

                               gCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATc                                                          >  2:318110/1‑93 (MQ=255)

                               gCCGAATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATc                                                          <  1:1227128/93‑1 (MQ=255)

                                  gCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACt                                                       >  2:1215323/1‑93 (MQ=255)

                                  gCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCAGGTGGCTTTTCCGGGCTGAAATCACt                                                       >  1:3343526/1‑93 (MQ=255)

                                   cATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTg                                                      <  1:1803/93‑1 (MQ=255)

                                    aTTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGa                                                     >  2:4242540/1‑93 (MQ=255)

                                    aTTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGa                                                     >  1:3789465/1‑93 (MQ=255)

                                     ttGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGaa                                                    >  1:1057419/1‑93 (MQ=255)

                                      tGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAAt                                                   <  1:167889/93‑1 (MQ=255)

                                       gaggagCGAACATTATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAAtt                                                  <  1:1764987/93‑1 (MQ=255)

                                       gaggagCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAAtt                                                  <  2:2314206/93‑1 (MQ=255)

                                       gaggagCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAAtt                                                  <  1:3212927/93‑1 (MQ=255)

                                        aggagCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAAttt                                                 <  1:3237232/93‑1 (MQ=255)

                                        aggagCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAAttt                                                 <  2:4088115/93‑1 (MQ=255)

                                           agCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCa                                              <  2:2934613/93‑1 (MQ=255)

                                           agCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAAGTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCa                                              <  2:604054/93‑1 (MQ=255)

                                            gCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAg                                             >  1:3568937/1‑93 (MQ=255)

                                               aaCATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTc                                          <  1:1883171/93‑1 (MQ=255)

                                                 catcatGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCtt                                        <  2:2868766/93‑1 (MQ=255)

                                                 catcatGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCtt                                        <  2:2737098/93‑1 (MQ=255)

                                                  atcatGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAACTCACTGAATTTGCAGGTCTTc                                       <  2:2986059/93‑1 (MQ=255)

                                                  atcatGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTc                                       <  2:174410/93‑1 (MQ=255)

                                                  atcatGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTc                                       <  1:1394861/93‑1 (MQ=255)

                                                   tcatGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCg                                      >  2:2186257/1‑93 (MQ=255)

                                                   tcatGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCg                                      <  2:2498614/93‑1 (MQ=255)

                                                   tcatGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCg                                      <  1:972262/93‑1 (MQ=255)

                                                   tcatGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCg                                      <  1:4113535/93‑1 (MQ=255)

                                                   tcatGTCGAAAAGCAATCCGTCTGAAGCGAAATTGGCCGCACCGACACCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTATTCg                                      >  1:73719/1‑93 (MQ=255)

                                                    catGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGTTTCTCCTGGCTGAAATCACTGAATTTGCAGGTCTTCGt                                     >  1:4682017/1‑93 (MQ=255)

                                                     atGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGtg                                    >  2:4664007/1‑93 (MQ=255)

                                                       gTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGtgat                                  >  2:2078282/1‑93 (MQ=255)

                                                        tCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGtgatg                                 >  1:3850248/1‑93 (MQ=255)

                                                        tCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGtgatg                                 >  2:483409/1‑93 (MQ=255)

                                                          gAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTgatgat                               <  2:453382/93‑1 (MQ=255)

                                                            aaaGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTg                             >  2:3272362/1‑93 (MQ=255)

                                                            aaaGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTg                             >  2:2442462/1‑93 (MQ=255)

                                                            aaaGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTg                             >  2:4458854/1‑93 (MQ=255)

                                                              aGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCa                           >  2:1061059/1‑93 (MQ=255)

                                                              aGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCa                           <  2:3384034/93‑1 (MQ=255)

                                                              aGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCa                           >  2:2466989/1‑93 (MQ=255)

                                                              aGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCa                           >  1:1520801/1‑93 (MQ=255)

                                                              aGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCa                           <  1:700978/93‑1 (MQ=255)

                                                               gCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAg                          <  2:335091/93‑1 (MQ=255)

                                                               gCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAg                          >  1:3840282/1‑93 (MQ=255)

                                                                cAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGc                         <  2:2558815/93‑1 (MQ=255)

                                                                cAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGc                         <  2:415198/93‑1 (MQ=255)

                                                                 aaTCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCt                        <  2:265179/93‑1 (MQ=255)

                                                                 aaTCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCt                        >  1:3104532/1‑93 (MQ=255)

                                                                  aTCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTa                       <  1:2887778/93‑1 (MQ=255)

                                                                  aTCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTa                       >  1:273403/1‑93 (MQ=255)

                                                                   tCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTat                      <  2:3691696/93‑1 (MQ=255)

                                                                   tCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTat                      >  1:3623024/1‑93 (MQ=255)

                                                                   tCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTat                      >  1:2162850/1‑93 (MQ=255)

                                                                   tCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTat                      <  1:2554385/93‑1 (MQ=255)

                                                                    ccGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTatc                     <  2:1714515/93‑1 (MQ=255)

                                                                     cGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTatca                    >  2:1116287/1‑93 (MQ=255)

                                                                     cGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTatca                    <  2:3843135/93‑1 (MQ=255)

                                                                      gTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTatcat                   <  1:2394080/93‑1 (MQ=255)

                                                                       tCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTatcatc                  >  1:528098/1‑93 (MQ=255)

                                                                        cTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCg                 >  1:3963730/1‑93 (MQ=255)

                                                                          gAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCa               >  1:3001990/1‑93 (MQ=255)

                                                                           aaGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCaa              >  2:3736666/1‑93 (MQ=255)

                                                                           aaGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCaa              <  2:2052753/93‑1 (MQ=255)

                                                                            aGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAAt             <  2:4420321/93‑1 (MQ=255)

                                                                             gTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATc            <  2:1273681/93‑1 (MQ=255)

                                                                              tGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTTCAGGTCTTCGTGATGATTGCAGCTATCATCTCAATCa           <  2:888161/93‑1 (MQ=255)

                                                                              tGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCa           <  2:1816591/93‑1 (MQ=255)

                                                                                aaaTTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATg         <  2:323857/93‑1 (MQ=255)

                                                                                 aaTTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGc        >  1:3321772/1‑93 (MQ=255)

                                                                                 aaTTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGc        <  1:2077082/93‑1 (MQ=255)

                                                                                  aTTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCt       <  1:3434701/93‑1 (MQ=255)

                                                                                  aTTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCt       >  1:1320387/1‑93 (MQ=255)

                                                                                   ttGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTg      <  2:3905697/93‑1 (MQ=255)

                                                                                   ttGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTg      >  2:4137181/1‑93 (MQ=255)

                                                                                    tGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGt     <  1:4428347/93‑1 (MQ=255)

                                                                                      gCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGttt   >  1:522941/1‑92 (MQ=255)

TCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCT  >  NC_000913/3731296‑3731475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTA  >  NC_000913/3731293‑3731478

AAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGC                                                                                              >  SRR4302535.2976948/1‑94 (MQ=60)

 AATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCA                                                                                             >  SRR4302535.730838/1‑94 (MQ=60)

  ATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCAC                                                                                            >  SRR4302535.1645086/1‑94 (MQ=60)

    CATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCG                                                                                          <  SRR4302535.4244145/94‑1 (MQ=60)

      TAGCCTGACTCAGGAGCCGTTGATGGACCCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGAC                                                                                        <  SRR4302535.1420488/94‑1 (MQ=60)

      TAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGAC                                                                                        <  SRR4302535.3083449/94‑1 (MQ=60)

       AACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACA                                                                                       >  SRR4302535.3338334/1‑94 (MQ=60)

       AACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACA                                                                                       >  SRR4302535.912621/1‑94 (MQ=60)

        ACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACAT                                                                                      <  SRR4302535.3554097/94‑1 (MQ=60)

        ACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACAT                                                                                      >  SRR4302535.3600627/1‑94 (MQ=60)

         CCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATC                                                                                     >  SRR4302535.509640/1‑94 (MQ=60)

         CCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCAGTCTGAAGTGAAATTGGCCGAACCGACAGC                                                                                     >  SRR4302535.101476/1‑94 (MQ=60)

          CTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCC                                                                                    >  SRR4302535.1492643/1‑94 (MQ=60)

            GACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGG                                                                                  <  SRR4302535.2322987/94‑1 (MQ=60)

             ACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGT                                                                                 >  SRR4302535.2639587/1‑94 (MQ=60)

              CTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTG                                                                                >  SRR4302535.2436748/1‑94 (MQ=60)

              CTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTG                                                                                <  SRR4302535.591945/94‑1 (MQ=60)

               TCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGG                                                                               >  SRR4302535.2700138/1‑94 (MQ=60)

                 AGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCT                                                                             >  SRR4302535.526654/1‑94 (MQ=60)

                 AGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCT                                                                             >  SRR4302535.1308886/1‑94 (MQ=60)

                  GGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTT                                                                            >  SRR4302535.1366068/1‑94 (MQ=60)

                    AGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCT                                                                          >  SRR4302535.577724/1‑94 (MQ=60)

                    AGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCT                                                                          <  SRR4302535.3985921/94‑1 (MQ=60)

                    AGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCT                                                                          <  SRR4302535.4274142/94‑1 (MQ=60)

                    AGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGCGGCTTCT                                                                          >  SRR4302535.4126349/1‑94 (MQ=60)

                      CAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCC                                                                        <  SRR4302535.2351848/94‑1 (MQ=60)

                      CAGGTAATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGGTTCTCC                                                                        <  SRR4302535.2076198/94‑1 (MQ=60)

                       AGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG                                                                       >  SRR4302535.2368696/1‑94 (MQ=60)

                       AGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG                                                                       <  SRR4302535.3386458/94‑1 (MQ=60)

                        GGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGG                                                                      >  SRR4302535.906230/1‑94 (MQ=60)

                           GATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCT                                                                   >  SRR4302535.2503801/1‑94 (MQ=60)

                           GATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCT                                                                   <  SRR4302535.772375/94‑1 (MQ=60)

                            ATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTG                                                                  >  SRR4302535.1520472/1‑94 (MQ=60)

                            ATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTG                                                                  >  SRR4302535.828681/1‑94 (MQ=60)

                            ATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTG                                                                  >  SRR4302535.1523497/1‑94 (MQ=60)

                               GAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAA                                                               <  SRR4302535.1635944/94‑1 (MQ=60)

                                AAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAAT                                                              >  SRR4302535.4497071/1‑94 (MQ=60)

                                AAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAAT                                                              <  SRR4302535.1051999/94‑1 (MQ=60)

                                 AGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATC                                                             <  SRR4302535.563078/94‑1 (MQ=60)

                                 AGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATC                                                             <  SRR4302535.4582014/94‑1 (MQ=60)

                                  GCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCA                                                            >  SRR4302535.1716006/1‑94 (MQ=60)

                                  GCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCA                                                            <  SRR4302535.3356438/94‑1 (MQ=60)

                                  GCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCA                                                            <  SRR4302535.2615946/94‑1 (MQ=60)

                                  GCCGAATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCA                                                            <  SRR4302535.1235902/94‑1 (MQ=60)

                                    CGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACT                                                          >  SRR4302535.3168589/1‑94 (MQ=60)

                                    CGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCAGGTGGCTTTTCCGGGCTGAAATCACT                                                          >  SRR4302535.3366820/1‑94 (MQ=60)

                                     GCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTG                                                         <  SRR4302535.1269168/94‑1 (MQ=60)

                                     GCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTG                                                         <  SRR4302535.66727/94‑1 (MQ=60)

                                      CATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGA                                                        <  SRR4302535.1822/94‑1 (MQ=60)

                                      CATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGA                                                        >  SRR4302535.2789674/1‑94 (MQ=60)

                                      CATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGA                                                        >  SRR4302535.3815601/1‑94 (MQ=60)

                                      CATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGCGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGA                                                        >  SRR4302535.333806/1‑94 (MQ=60)

                                       ATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAA                                                       >  SRR4302535.1064938/1‑94 (MQ=60)

                                       ATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAA                                                       >  SRR4302535.1708008/1‑94 (MQ=60)

                                       ATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAA                                                       <  SRR4302535.3876396/94‑1 (MQ=60)

                                        TTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAAT                                                      <  SRR4302535.735201/94‑1 (MQ=60)

                                         TGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATT                                                     <  SRR4302535.169053/94‑1 (MQ=60)

                                          GAGGAGCGAACATTATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTT                                                    <  SRR4302535.1777781/94‑1 (MQ=60)

                                          GAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTT                                                    <  SRR4302535.3235179/94‑1 (MQ=60)

                                           AGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTG                                                   <  SRR4302535.3259706/94‑1 (MQ=60)

                                           AGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTG                                                   <  SRR4302535.3625824/94‑1 (MQ=60)

                                           AGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTG                                                   <  SRR4302535.2314031/94‑1 (MQ=60)

                                           AGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTG                                                   <  SRR4302535.802465/94‑1 (MQ=60)

                                              AGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAG                                                >  SRR4302535.3593687/1‑94 (MQ=60)

                                              AGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAG                                                <  SRR4302535.3378435/94‑1 (MQ=60)

                                              AGAGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAG                                                <  SRR4302535.1865484/94‑1 (MQ=60)

                                               GCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGG                                               <  SRR4302535.3652160/94‑1 (MQ=60)

                                                  AACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCT                                            <  SRR4302535.1488554/94‑1 (MQ=60)

                                                  AACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCT                                            <  SRR4302535.1896744/94‑1 (MQ=60)

                                                     ATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCG                                         <  SRR4302535.2837419/94‑1 (MQ=60)

                                                     ATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCG                                         <  SRR4302535.1404821/94‑1 (MQ=60)

                                                     ATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCG                                         <  SRR4302535.255119/94‑1 (MQ=60)

                                                     ATCATGTCGAAAAGCAATCCGTCTGAAGCGAAATTGGCCGCACCGACACCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTATTCG                                         >  SRR4302535.74254/1‑94 (MQ=60)

                                                      TCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGTTTCTCCTGGCTGAAATCACTGAATTTGCAGGTCTTCGT                                        >  SRR4302535.4713659/1‑94 (MQ=60)

                                                      TCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGT                                        <  SRR4302535.4141691/94‑1 (MQ=60)

                                                      TCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGT                                        <  SRR4302535.979159/94‑1 (MQ=60)

                                                       CATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTG                                       <  SRR4302535.2588656/94‑1 (MQ=60)

                                                       CATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTG                                       >  SRR4302535.2599363/1‑94 (MQ=60)

                                                         TGTGGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGAT                                     <  SRR4302535.2446461/94‑1 (MQ=60)

                                                          GTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATG                                    >  SRR4302535.3876743/1‑94 (MQ=60)

                                                           TCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGA                                   <  SRR4302535.3849289/94‑1 (MQ=60)

                                                             GAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATT                                 >  SRR4302535.3245625/1‑94 (MQ=60)

                                                                AAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCA                              >  SRR4302535.1531755/1‑94 (MQ=60)

                                                                 AGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAG                             <  SRR4302535.1233321/94‑1 (MQ=60)

                                                                 AGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAG                             <  SRR4302535.705892/94‑1 (MQ=60)

                                                                 AGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAG                             >  SRR4302535.3866746/1‑94 (MQ=60)

                                                                   CAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCT                           >  SRR4302535.3126147/1‑94 (MQ=60)

                                                                    AATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTA                          >  SRR4302535.275308/1‑94 (MQ=60)

                                                                    AATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTA                          >  SRR4302535.4338956/1‑94 (MQ=60)

                                                                     ATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTAT                         >  SRR4302535.1510873/1‑94 (MQ=60)

                                                                     ATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTAT                         >  SRR4302535.3648138/1‑94 (MQ=60)

                                                                     ATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTAT                         >  SRR4302535.2178368/1‑94 (MQ=60)

                                                                     ATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTAT                         <  SRR4302535.2908210/94‑1 (MQ=60)

                                                                     ATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTAT                         <  SRR4302535.1627272/94‑1 (MQ=60)

                                                                      TCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATC                        <  SRR4302535.2572776/94‑1 (MQ=60)

                                                                        CGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCAT                      <  SRR4302535.4211186/94‑1 (MQ=60)

                                                                        CGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCAT                      >  SRR4302535.922424/1‑94 (MQ=60)

                                                                        CGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCAT                      >  SRR4302535.180504/1‑94 (MQ=60)

                                                                         GTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATC                     >  SRR4302535.531788/1‑94 (MQ=60)

                                                                         GTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATC                     >  SRR4302535.2975689/1‑94 (MQ=60)

                                                                         GTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATC                     <  SRR4302535.2411290/94‑1 (MQ=60)

                                                                          TCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCG                    >  SRR4302535.3990807/1‑94 (MQ=60)

                                                                           CTGAAGTGAAATTTGCCGCACCGACATCCGGTGGCTTCTCCGGGgtggaataactgaaaattcaggcctcgctgtggtttttagctattactcc                   >  SRR4302535.3043523/1‑44 (MQ=60)

                                                                           CTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGC                   >  SRR4302535.3563391/1‑94 (MQ=60)

                                                                            TGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCA                  >  SRR4302535.2333069/1‑94 (MQ=60)

                                                                            TGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCA                  >  SRR4302535.3023034/1‑94 (MQ=60)

                                                                                GTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCA              <  SRR4302535.4020561/94‑1 (MQ=60)

                                                                                   AAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGC           >  SRR4302535.446466/1‑94 (MQ=60)

                                                                                   AAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGC           >  SRR4302535.3344882/1‑94 (MQ=60)

                                                                                    AATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCT          <  SRR4302535.2172276/94‑1 (MQ=60)

                                                                                    AATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCT          <  SRR4302535.2092045/94‑1 (MQ=60)

                                                                                    AATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCT          <  SRR4302535.886859/94‑1 (MQ=60)

                                                                                    AATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCT          >  SRR4302535.3044320/1‑94 (MQ=60)

                                                                                    AATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCT          >  SRR4302535.1329819/1‑94 (MQ=60)

                                                                                     ATTGGCCGCACCTACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATGGCAGCTATCATCGCAATCATGCTG         <  SRR4302535.1622505/94‑1 (MQ=60)

                                                                                     ATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTG         <  SRR4302535.1974095/94‑1 (MQ=60)

                                                                                     ATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTG         <  SRR4302535.3458571/94‑1 (MQ=60)

                                                                                      TTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGT        >  SRR4302535.3473050/1‑94 (MQ=60)

                                                                                       TGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTT       >  SRR4302535.2272820/1‑94 (MQ=60)

                                                                                       TGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTT       <  SRR4302535.4458683/94‑1 (MQ=60)

                                                                                       TGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTT       >  SRR4302535.2751241/1‑94 (MQ=60)

                                                                                       TGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTT       <  SRR4302535.3846171/94‑1 (MQ=60)

                                                                                        GGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTT      >  SRR4302535.526600/1‑94 (MQ=60)

                                                                                          CCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTT    <  SRR4302535.496073/94‑1 (MQ=60)

                                                                                           CGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTT   <  SRR4302535.1636917/94‑1 (MQ=60)

                                                                                            GCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTA  <  SRR4302535.4439394/94‑1 (MQ=60)

AAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTA  >  NC_000913/3731293‑3731478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`