Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,731,386 T→C V13A (GTA→GCA)  xylH → XylH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,731,3860TC99.5% 1217.7 / ‑4.1 389V13A (GTA→GCA) xylHXylH
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (200/187);  total (201/188)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 405 total aligned reads displayed.

TCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCT  >  NC_000913/3731296‑3731475
                                                                                          |                                                                                         
tCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCAc                                                                                         <  1:7106328/93‑1 (MQ=255)
 cATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCAcc                                                                                        <  1:2649669/93‑1 (MQ=255)
 cATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCAcc                                                                                        <  1:7797602/93‑1 (MQ=255)
  aTAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCg                                                                                       >  2:7717123/1‑93 (MQ=255)
   tAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGa                                                                                      <  2:2313187/93‑1 (MQ=255)
   tAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGa                                                                                      >  1:3424885/1‑93 (MQ=255)
     aCCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACa                                                                                    <  1:4166810/93‑1 (MQ=255)
     aCCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACa                                                                                    >  1:299608/1‑93 (MQ=255)
     aCCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACa                                                                                    <  1:978177/93‑1 (MQ=255)
       ctatcTCACGATCAGGTGATGGACGCCGCATTGAGGAGCGATCATCATGTCGAAAGGCAATCCGTCTGAAGTGAAATTGGCCGCGCCGGCATc                                                                                  <  2:3350386/89‑1 (MQ=255)
       cTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATc                                                                                  <  1:940124/93‑1 (MQ=255)
        tGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATcc                                                                                 >  1:195368/1‑93 (MQ=255)
          aCTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCgg                                                                               <  1:5519944/93‑1 (MQ=255)
          aCTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCgg                                                                               <  1:3188229/93‑1 (MQ=255)
           cTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGt                                                                              <  2:7574671/93‑1 (MQ=255)
           atCAGGACCAGGTGATGGAAGCCGCAGTGAGGAGCGAACATCATGTCGAAAATCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGt                                                                              <  1:6715269/92‑1 (MQ=255)
             cAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTgg                                                                            <  1:1142259/93‑1 (MQ=255)
             cAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTgg                                                                            <  1:5949171/93‑1 (MQ=255)
              aGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGc                                                                           >  1:389559/1‑93 (MQ=255)
              aGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGc                                                                           >  2:2742276/1‑93 (MQ=255)
               ggAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCt                                                                          >  2:636444/1‑93 (MQ=255)
                gAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCtt                                                                         >  1:5809346/1‑93 (MQ=255)
                  gCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTtct                                                                       <  1:4734069/93‑1 (MQ=255)
                    agtggATGCAAGCCGAATTGAGTAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCGTCTcc                                                                     <  2:2480992/89‑1 (MQ=255)
                     ggTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCAGCGACATCCGGTGGCTTCTCCg                                                                    <  2:4469517/93‑1 (MQ=255)
                     ggTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCg                                                                    >  1:3548501/1‑93 (MQ=255)
                     ggTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCg                                                                    <  2:3855018/93‑1 (MQ=255)
                     ggTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCg                                                                    <  2:807594/93‑1 (MQ=255)
                      gTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCgg                                                                   <  1:167070/93‑1 (MQ=255)
                      gTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGAGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCgg                                                                   >  2:6002737/1‑93 (MQ=255)
                        gATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGc                                                                 <  2:2358648/93‑1 (MQ=255)
                          tGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGTCCGCACCGACATCCGGCGGCTTCTCCGGGCTg                                                               >  2:1073682/1‑93 (MQ=255)
                          tGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTg                                                               >  1:2358784/1‑93 (MQ=255)
                            gAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGaa                                                             <  1:1014923/93‑1 (MQ=255)
                               gCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATc                                                          <  1:6316533/93‑1 (MQ=255)
                                 cGCATTGAGGAGCGAACATCATGTCGAAAAGCAACCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCAc                                                        >  2:6302892/1‑93 (MQ=255)
                                  gCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACt                                                       >  2:4595656/1‑93 (MQ=255)
                                    aTTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGa                                                     >  2:7530421/1‑93 (MQ=255)
                                    aTTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGa                                                     >  2:6665996/1‑93 (MQ=255)
                                     ttGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGaa                                                    >  2:2111785/1‑93 (MQ=255)
                                       gaggagCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGTCGCACCGACATCGGGTGGCTTCTCCGGGCTGAAATCACTGAAtt                                                  >  1:2088660/1‑93 (MQ=255)
                                        aggagCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAAttt                                                 <  2:7308071/93‑1 (MQ=255)
                                        aggagCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAAttt                                                 >  1:7539519/1‑93 (MQ=255)
                                        aggagCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAAttt                                                 <  2:1895725/93‑1 (MQ=255)
                                         ggagCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTg                                                <  2:4291080/93‑1 (MQ=255)
                                           agCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCa                                              >  2:6908274/1‑93 (MQ=255)
                                            gCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCGGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAg                                             <  2:3746417/93‑1 (MQ=255)
                                              gAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGt                                           >  1:4313067/1‑93 (MQ=255)
                                               aaCATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTc                                          <  2:149186/93‑1 (MQ=255)
                                               aaCATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTc                                          >  1:4536996/1‑93 (MQ=255)
                                                aCATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCt                                         <  1:2121369/93‑1 (MQ=255)
                                                aCATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCt                                         >  1:7293451/1‑93 (MQ=255)
                                                aCATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCt                                         >  1:1357695/1‑93 (MQ=255)
                                                aCATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCt                                         >  1:4847220/1‑93 (MQ=255)
                                                 catcatGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGGAATCACTGAATTTGCAGGtttt                                        >  1:7894579/1‑93 (MQ=255)
                                                  atcatGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGCAATCACTGAATTTGCAGGTCTTc                                       >  2:566068/1‑93 (MQ=255)
                                                  atcatGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTc                                       <  2:5521125/93‑1 (MQ=255)
                                                  atcatGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTc                                       >  2:5389725/1‑93 (MQ=255)
                                                   tcatGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCg                                      >  2:7067836/1‑93 (MQ=255)
                                                   tcatGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCg                                      <  2:5847315/93‑1 (MQ=255)
                                                   tcatGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCg                                      <  2:4013137/93‑1 (MQ=255)
                                                    catGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGt                                     <  1:6463651/93‑1 (MQ=255)
                                                       gTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGTATTTGCAGGTCTTCGtgct                                  >  2:4868896/1‑91 (MQ=255)
                                                       gTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGtgat                                  >  1:5075033/1‑93 (MQ=255)
                                                         cGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGtgatga                                <  2:7835857/93‑1 (MQ=255)
                                                          gAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTgatgat                               >  1:3772007/1‑93 (MQ=255)
                                                           aaaaGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGAtt                              <  1:2474650/93‑1 (MQ=255)
                                                           aaaaGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGAtt                              >  2:5145135/1‑93 (MQ=255)
                                                             aaGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGc                            >  2:3064633/1‑93 (MQ=255)
                                                              aGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCa                           <  2:4352120/93‑1 (MQ=255)
                                                              aGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCa                           >  2:5488672/1‑93 (MQ=255)
                                                              aGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCa                           >  1:4622626/1‑93 (MQ=255)
                                                                cAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGc                         >  1:7647513/1‑93 (MQ=255)
                                                                 aaTCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCt                        >  1:1601862/1‑93 (MQ=255)
                                                                  aTCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTa                       <  1:4087229/93‑1 (MQ=255)
                                                                  aTCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTa                       <  2:7143296/93‑1 (MQ=255)
                                                                   tCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTat                      >  1:2290980/1‑93 (MQ=255)
                                                                   tCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTat                      >  2:7240104/1‑93 (MQ=255)
                                                                   tCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTat                      >  2:1792959/1‑93 (MQ=255)
                                                                    ccGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTatc                     <  2:7006803/93‑1 (MQ=255)
                                                                     cGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTatca                    <  1:1254858/93‑1 (MQ=255)
                                                                     cGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTatca                    >  1:3318515/1‑93 (MQ=255)
                                                                      gTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTatcat                   <  1:4944315/93‑1 (MQ=255)
                                                                      gTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTatcat                   <  2:6749181/93‑1 (MQ=255)
                                                                       tCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTatcatc                  >  1:5368876/1‑93 (MQ=255)
                                                                       tCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTatcatc                  >  2:281907/1‑93 (MQ=255)
                                                                        cTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCg                 <  2:2418451/93‑1 (MQ=255)
                                                                        cTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCg                 >  2:1317599/1‑93 (MQ=255)
                                                                         tGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGc                >  1:6874557/1‑93 (MQ=255)
                                                                         tGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGc                <  1:1118452/93‑1 (MQ=255)
                                                                         tGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGATCTTCGTGATGATTGCAGCTATCATCGc                >  1:7192087/1‑93 (MQ=255)
                                                                            aGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGCATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAAt             >  2:1579443/1‑93 (MQ=255)
                                                                            aGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAAt             >  2:5001182/1‑93 (MQ=255)
                                                                              tGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCa           <  1:7012238/93‑1 (MQ=255)
                                                                                aaaTTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATg         <  1:172180/93‑1 (MQ=255)
                                                                                  aTTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCt       >  1:5678063/1‑93 (MQ=255)
                                                                                    tGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGt     >  2:473158/1‑93 (MQ=255)
                                                                                     ggCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGtt    >  1:6965088/1‑93 (MQ=255)
                                                                                      gCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTc   <  1:1532554/93‑1 (MQ=255)
                                                                                       ccGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCt  <  1:6197236/93‑1 (MQ=255)
                                                                                          |                                                                                         
TCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCT  >  NC_000913/3731296‑3731475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 230 total aligned reads displayed.
AAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTAC  >  NC_000913/3731293‑3731479
                                                                                             |                                                                                             
AAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGC                                                                                               >  SRR4302543.7428574/1‑94 (MQ=60)
 AATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCA                                                                                              <  SRR4302543.1668844/94‑1 (MQ=60)
 AATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCA                                                                                              >  SRR4302543.2747406/1‑94 (MQ=60)
 AATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCA                                                                                              <  SRR4302543.1565516/94‑1 (MQ=60)
  ATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCAC                                                                                             <  SRR4302543.1569617/94‑1 (MQ=60)
   TCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACC                                                                                            >  SRR4302543.7925392/1‑94 (MQ=60)
   TCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACC                                                                                            <  SRR4302543.7175356/94‑1 (MQ=60)
   TCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACC                                                                                            <  SRR4302543.4642064/94‑1 (MQ=60)
   TCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACC                                                                                            <  SRR4302543.4642065/94‑1 (MQ=60)
    CATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCG                                                                                           <  SRR4302543.1427620/94‑1 (MQ=60)
    CATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCG                                                                                           <  SRR4302543.2673691/94‑1 (MQ=60)
     ATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGC                                                                                          >  SRR4302543.4176295/1‑94 (MQ=60)
     ATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGA                                                                                          >  SRR4302543.3455533/1‑94 (MQ=60)
     ATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGA                                                                                          >  SRR4302543.4726367/1‑94 (MQ=60)
     ATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGA                                                                                          <  SRR4302543.2006320/94‑1 (MQ=60)
       AACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACA                                                                                        >  SRR4302543.302299/1‑94 (MQ=60)
       AACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACA                                                                                        <  SRR4302543.3477641/94‑1 (MQ=60)
        ACCTGACTCAGGAGCAGGTGATGGAAGCCTCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACAT                                                                                       <  SRR4302543.3233516/94‑1 (MQ=60)
        ACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACAT                                                                                       <  SRR4302543.986813/94‑1 (MQ=60)
        ACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACAT                                                                                       <  SRR4302543.4204540/94‑1 (MQ=60)
         CCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATC                                                                                      >  SRR4302543.5920291/1‑94 (MQ=60)
         CCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATC                                                                                      >  SRR4302543.112076/1‑94 (MQ=60)
          CTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCC                                                                                     <  SRR4302543.948337/94‑1 (MQ=60)
          CTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCC                                                                                     >  SRR4302543.197015/1‑94 (MQ=60)
            GACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGG                                                                                   <  SRR4302543.723437/94‑1 (MQ=60)
            GACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCAGCTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGG                                                                                   <  SRR4302543.2365620/94‑1 (MQ=60)
             ACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGT                                                                                  <  SRR4302543.5570080/94‑1 (MQ=60)
             ACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGT                                                                                  <  SRR4302543.3216978/94‑1 (MQ=60)
             ACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGT                                                                                  <  SRR4302543.2114519/94‑1 (MQ=60)
              CTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAAGTGGCCGCACCGACATCGGGTG                                                                                 >  SRR4302543.2366282/1‑94 (MQ=60)
              ATCAGGACCAGGTGATGGAAGCCGCAGTGAGGAGCGAACATCATGTCGAAAATCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTG                                                                                 <  SRR4302543.6778733/94‑1 (MQ=60)
               TCAGGAGCAGGTGATGGAAGCTGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGG                                                                                <  SRR4302543.2264454/94‑1 (MQ=60)
               TCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGG                                                                                <  SRR4302543.4435347/94‑1 (MQ=60)
                CAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGC                                                                               <  SRR4302543.6003614/94‑1 (MQ=60)
                CAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGC                                                                               >  SRR4302543.393157/1‑94 (MQ=60)
                CAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGC                                                                               <  SRR4302543.1152665/94‑1 (MQ=60)
                CAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGC                                                                               <  SRR4302543.1603997/94‑1 (MQ=60)
                CAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGC                                                                               <  SRR4302543.2550169/94‑1 (MQ=60)
                 actggcacGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCT                                                                              <  SRR4302543.7075483/86‑1 (MQ=60)
                 GGGAGCGGGTGGTGGAAGCCGCATTGAGGAGCGATCATCGAGTCGAAAGGCAATCCGTCTGAAGTACAATTGGCCGCACCGCCATCCGGTGGCT                                                                              <  SRR4302543.7075484/94‑1 (MQ=40)
                  GGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGGTT                                                                             <  SRR4302543.4271434/94‑1 (MQ=60)
                  GGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTT                                                                             >  SRR4302543.5862180/1‑94 (MQ=60)
                    GGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCT                                                                           <  SRR4302543.1701797/94‑1 (MQ=60)
                    AGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCT                                                                           <  SRR4302543.806846/94‑1 (MQ=60)
                     GCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTC                                                                          <  SRR4302543.4777334/94‑1 (MQ=60)
                      CAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCC                                                                         <  SRR4302543.1037248/94‑1 (MQ=60)
                      CAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCC                                                                         >  SRR4302543.4921405/1‑94 (MQ=60)
                       AGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG                                                                        >  SRR4302543.3579992/1‑94 (MQ=60)
                       AGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG                                                                        <  SRR4302543.6918774/94‑1 (MQ=60)
                       AGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG                                                                        <  SRR4302543.7020473/94‑1 (MQ=60)
                       AGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG                                                                        <  SRR4302543.7078751/94‑1 (MQ=60)
                       AGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG                                                                        >  SRR4302543.7137396/1‑94 (MQ=60)
                        GGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGG                                                                       >  SRR4302543.1074420/1‑94 (MQ=60)
                         GTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGG                                                                      <  SRR4302543.7459398/94‑1 (MQ=60)
                         GTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGG                                                                      <  SRR4302543.168510/94‑1 (MQ=60)
                           GATGGAAGCTGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCT                                                                    <  SRR4302543.63263/94‑1 (MQ=60)
                           GATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCT                                                                    <  SRR4302543.5631731/94‑1 (MQ=60)
                            ATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTG                                                                   >  SRR4302543.2380173/1‑94 (MQ=60)
                               GAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAA                                                                <  SRR4302543.1023903/94‑1 (MQ=60)
                                AAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAAT                                                               <  SRR4302543.2136471/94‑1 (MQ=60)
                                AAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAAT                                                               <  SRR4302543.6624637/94‑1 (MQ=60)
                                AAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAAT                                                               <  SRR4302543.380253/94‑1 (MQ=60)
                                AAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAAT                                                               >  SRR4302543.3910999/1‑94 (MQ=60)
                                AAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAAT                                                               <  SRR4302543.767086/94‑1 (MQ=60)
                                AAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAAT                                                               >  SRR4302543.7445864/1‑94 (MQ=60)
                                 AGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATC                                                              <  SRR4302543.1246578/94‑1 (MQ=60)
                                  agCGCATTGAGGAGCGAACATCATGTCTAAAAGCAATCCGTCTTAAGTTAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCA                                                             <  SRR4302543.976157/92‑1 (MQ=60)
                                  GCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCA                                                             <  SRR4302543.975935/94‑1 (MQ=60)
                                   CCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCAC                                                            <  SRR4302543.3935861/94‑1 (MQ=60)
                                    CGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACT                                                           <  SRR4302543.3444134/94‑1 (MQ=60)
                                    CGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACT                                                           >  SRR4302543.2909692/1‑94 (MQ=60)
                                     GCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAGATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTA                                                          >  SRR4302543.2612317/1‑94 (MQ=60)
                                     GCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTG                                                          <  SRR4302543.7180851/94‑1 (MQ=60)
                                     GCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTG                                                          <  SRR4302543.2114498/94‑1 (MQ=60)
                                     GCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTG                                                          <  SRR4302543.7639160/94‑1 (MQ=60)
                                      CATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGA                                                         >  SRR4302543.3250648/1‑94 (MQ=60)
                                       ATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAT                                                        >  SRR4302543.1152726/1‑94 (MQ=60)
                                         TGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGTCGCACCGACATCGGGTGGCTTCTCCGGGCTGAAATCACTGAATT                                                      >  SRR4302543.2107323/1‑94 (MQ=60)
                                         TGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATT                                                      >  SRR4302543.1583262/1‑94 (MQ=60)
                                          GAGGAGCCAACATCATGTCGAAAATCAATCCGTCTGAAGTGAAATTGGCCGCTCCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTT                                                     <  SRR4302543.1411684/94‑1 (MQ=60)
                                           AGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTG                                                    >  SRR4302543.1668611/1‑94 (MQ=60)
                                           AGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTG                                                    >  SRR4302543.7904076/1‑94 (MQ=60)
                                           AGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTG                                                    <  SRR4302543.5426307/94‑1 (MQ=60)
                                           AGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTG                                                    <  SRR4302543.80917/94‑1 (MQ=60)
                                           AGGAGCGAACATCATGTCGAAAAGCAAGCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTG                                                    <  SRR4302543.1860161/94‑1 (MQ=60)
                                             GAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCA                                                  <  SRR4302543.6470951/94‑1 (MQ=60)
                                              AGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAG                                                 <  SRR4302543.6267995/94‑1 (MQ=60)
                                              AGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAG                                                 <  SRR4302543.4493751/94‑1 (MQ=60)
                                              AGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAG                                                 <  SRR4302543.7164394/94‑1 (MQ=60)
                                              AGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAG                                                 >  SRR4302543.3179543/1‑94 (MQ=60)
                                              AGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAG                                                 <  SRR4302543.4098654/94‑1 (MQ=60)
                                              AGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAG                                                 <  SRR4302543.635657/94‑1 (MQ=60)
                                               GCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGG                                                <  SRR4302543.4882435/94‑1 (MQ=60)
                                                CGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGT                                               >  SRR4302543.6398620/1‑94 (MQ=60)
                                                 GAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTC                                              >  SRR4302543.2343666/1‑94 (MQ=60)
                                                 GAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTC                                              >  SRR4302543.4578295/1‑94 (MQ=60)
                                                  AACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCT                                             >  SRR4302543.1369844/1‑94 (MQ=60)
                                                  AACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCT                                             >  SRR4302543.1369843/1‑94 (MQ=60)
                                                  AACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCT                                             >  SRR4302543.5799394/1‑94 (MQ=60)
                                                  AACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCT                                             >  SRR4302543.7363836/1‑94 (MQ=60)
                                                   ACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGGAATCACTGAATTTGCAGGTTTT                                            >  SRR4302543.7968815/1‑94 (MQ=60)
                                                   ACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTT                                            <  SRR4302543.2140363/94‑1 (MQ=60)
                                                      tgataTCGAAAAGCAATCCGTATGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGT                                         <  SRR4302543.1586408/89‑1 (MQ=60)
                                                      atATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGT                                         <  SRR4302543.1607/92‑1 (MQ=60)
                                                       CATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTG                                        <  SRR4302543.6524549/94‑1 (MQ=60)
                                                       CATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTG                                        >  SRR4302543.4791148/1‑94 (MQ=60)
                                                       CATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTG                                        >  SRR4302543.2819102/1‑94 (MQ=60)
                                                        ATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGA                                       <  SRR4302543.1805163/94‑1 (MQ=60)
                                                         TGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGAT                                      <  SRR4302543.4994012/94‑1 (MQ=60)
                                                         TGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGAT                                      >  SRR4302543.5121089/1‑94 (MQ=60)
                                                          GTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATG                                     >  SRR4302543.6965703/1‑94 (MQ=60)
                                                          GTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATG                                     <  SRR4302543.404768/94‑1 (MQ=60)
                                                            CGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGAT                                   >  SRR4302543.3563283/1‑94 (MQ=60)
                                                            CGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGAT                                   >  SRR4302543.984541/1‑94 (MQ=60)
                                                            CGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGAT                                   >  SRR4302543.3805218/1‑94 (MQ=60)
                                                            CGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGAT                                   >  SRR4302543.3805219/1‑94 (MQ=60)
                                                             GAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATT                                  <  SRR4302543.1702515/94‑1 (MQ=60)
                                                             GAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTGGCAGGTCTTCGTGATGATT                                  >  SRR4302543.7767058/1‑94 (MQ=60)
                                                              AAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTG                                 <  SRR4302543.98561/94‑1 (MQ=60)
                                                              AAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTG                                 >  SRR4302543.2701601/1‑94 (MQ=60)
                                                              AAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTG                                 <  SRR4302543.2497019/94‑1 (MQ=60)
                                                              AAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCCGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTG                                 >  SRR4302543.1146600/1‑94 (MQ=60)
                                                               AAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGC                                >  SRR4302543.2446644/1‑94 (MQ=60)
                                                                AAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCA                               >  SRR4302543.6707685/1‑94 (MQ=60)
                                                                AAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCA                               >  SRR4302543.6248110/1‑94 (MQ=60)
                                                                AAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCA                               <  SRR4302543.6242614/94‑1 (MQ=60)
                                                                 AGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAG                              <  SRR4302543.1450708/94‑1 (MQ=60)
                                                                 AGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAG                              >  SRR4302543.7262888/1‑94 (MQ=60)
                                                                 AGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAG                              <  SRR4302543.4131596/94‑1 (MQ=60)
                                                                  GCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGC                             >  SRR4302543.7187962/1‑94 (MQ=60)
                                                                  GCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGC                             >  SRR4302543.7971716/1‑94 (MQ=60)
                                                                  GCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGC                             <  SRR4302543.566855/94‑1 (MQ=60)
                                                                  GCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGC                             >  SRR4302543.7720029/1‑94 (MQ=60)
                                                                   CAATCTGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCT                            <  SRR4302543.2428310/94‑1 (MQ=60)
                                                                   CAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCT                            >  SRR4302543.1616163/1‑94 (MQ=60)
                                                                   CAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCT                            >  SRR4302543.1490752/1‑94 (MQ=60)
                                                                   CAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCT                            >  SRR4302543.1660260/1‑94 (MQ=60)
                                                                   CAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCT                            >  SRR4302543.7701544/1‑94 (MQ=60)
                                                                    AATCCGTCTGAAGTGAAATTGGCCGCACCGCCATCCGGTGGGTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTA                           >  SRR4302543.1947917/1‑94 (MQ=60)
                                                                    AATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTA                           >  SRR4302543.4278560/1‑94 (MQ=60)
                                                                     attcggcttaaggtAAATTGGGCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTAT                          <  SRR4302543.2720325/80‑1 (MQ=60)
                                                                     ATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTAT                          >  SRR4302543.4643717/1‑94 (MQ=60)
                                                                     ATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTAT                          >  SRR4302543.2311890/1‑94 (MQ=60)
                                                                     ATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTAT                          <  SRR4302543.6780681/94‑1 (MQ=60)
                                                                     ATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTAT                          <  SRR4302543.4123877/94‑1 (MQ=60)
                                                                       CCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCA                        >  SRR4302543.3405059/1‑94 (MQ=60)
                                                                       CCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCA                        <  SRR4302543.7877394/94‑1 (MQ=60)
                                                                        CGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCAT                       >  SRR4302543.3125911/1‑94 (MQ=60)
                                                                        CGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCAT                       >  SRR4302543.3542752/1‑94 (MQ=60)
                                                                        CGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCAT                       <  SRR4302543.1266104/94‑1 (MQ=60)
                                                                         GTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATC                      >  SRR4302543.5994965/1‑94 (MQ=60)
                                                                         GTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATC                      >  SRR4302543.5189503/1‑94 (MQ=60)
                                                                         GTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATC                      >  SRR4302543.5417484/1‑94 (MQ=60)
                                                                         GTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATC                      <  SRR4302543.4989343/94‑1 (MQ=60)
                                                                         ATCTGAAGTGAAATTGGCCGCGCCGACATCCGGTGGCTGCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATC                      <  SRR4302543.6522607/94‑1 (MQ=60)
                                                                          TCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCG                     <  SRR4302543.3755676/94‑1 (MQ=60)
                                                                          TCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCG                     <  SRR4302543.197737/94‑1 (MQ=60)
                                                                          TCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCG                     >  SRR4302543.7741063/1‑94 (MQ=60)
                                                                          TCTGAAGTCAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCG                     <  SRR4302543.6860158/94‑1 (MQ=60)
                                                                           CTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGC                    >  SRR4302543.3271515/1‑94 (MQ=60)
                                                                           CTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGC                    >  SRR4302543.734386/1‑94 (MQ=60)
                                                                           CTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGC                    >  SRR4302543.6939284/1‑94 (MQ=60)
                                                                           CTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGC                    >  SRR4302543.5903417/1‑94 (MQ=60)
                                                                           CTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGC                    >  SRR4302543.2248716/1‑94 (MQ=60)
                                                                           CTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGATCTTCGTGATGATTGCAGCTATCATCGC                    >  SRR4302543.7261837/1‑94 (MQ=60)
                                                                            TGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCA                   <  SRR4302543.1128589/94‑1 (MQ=60)
                                                                            TGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCA                   <  SRR4302543.1128588/94‑1 (MQ=60)
                                                                            TGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCA                   <  SRR4302543.4727540/94‑1 (MQ=60)
                                                                            TGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCA                   >  SRR4302543.1272089/1‑94 (MQ=60)
                                                                            TGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGAGCTGAAATCTCTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCA                   <  SRR4302543.4727539/94‑1 (MQ=60)
                                                                             GAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAA                  >  SRR4302543.4998427/1‑94 (MQ=60)
                                                                              AAGTGAAATTGGCCGCACCAACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAAT                 >  SRR4302543.2584723/1‑94 (MQ=60)
                                                                               AGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATC                >  SRR4302543.3872911/1‑94 (MQ=60)
                                                                               AGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATC                >  SRR4302543.5141130/1‑94 (MQ=60)
                                                                               AGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATC                <  SRR4302543.7515543/94‑1 (MQ=60)
                                                                                 TGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCAT              <  SRR4302543.1734480/94‑1 (MQ=60)
                                                                                 TGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCAT              <  SRR4302543.7079125/94‑1 (MQ=60)
                                                                                 TGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCAT              <  SRR4302543.4842688/94‑1 (MQ=60)
                                                                                 TGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCAT              >  SRR4302543.4234215/1‑94 (MQ=60)
                                                                                 TGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCAT              <  SRR4302543.3680458/94‑1 (MQ=60)
                                                                                 TGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGGCTTCGTGATGATTGCAGCTATCATCGCAATCAT              >  SRR4302543.4502581/1‑94 (MQ=60)
                                                                                  GAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATG             >  SRR4302543.55929/1‑94 (MQ=60)
                                                                                  GAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATG             <  SRR4302543.4988127/94‑1 (MQ=60)
                                                                                    AATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAGTCATGCT           <  SRR4302543.7867825/94‑1 (MQ=60)
                                                                                    AATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCT           >  SRR4302543.5729891/1‑94 (MQ=60)
                                                                                    AATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCT           >  SRR4302543.5729892/1‑94 (MQ=60)
                                                                                     ATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTT          >  SRR4302543.1168191/1‑94 (MQ=60)
                                                                                     ATTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTG          >  SRR4302543.5427593/1‑94 (MQ=60)
                                                                                      TTGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGT         <  SRR4302543.6538796/94‑1 (MQ=60)
                                                                                       TGGCCGCACCGACATCCGGTGTCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGTTATCATCGCAATCATGCTGTT        <  SRR4302543.4913880/94‑1 (MQ=60)
                                                                                       TGGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTT        >  SRR4302543.7030633/1‑94 (MQ=60)
                                                                                        GGCCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTC       >  SRR4302543.5245038/1‑94 (MQ=60)
                                                                                          CCGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTT     <  SRR4302543.6255770/94‑1 (MQ=60)
                                                                                           CGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTT    >  SRR4302543.2093511/1‑94 (MQ=60)
                                                                                           CGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTT    >  SRR4302543.2059649/1‑94 (MQ=60)
                                                                                           CGCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTT    >  SRR4302543.3315574/1‑94 (MQ=60)
                                                                                            GCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTA   <  SRR4302543.6294422/94‑1 (MQ=60)
                                                                                            GCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTA   <  SRR4302543.1046650/94‑1 (MQ=60)
                                                                                            GCACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTA   <  SRR4302543.5533174/94‑1 (MQ=60)
                                                                                             CACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTAC  >  SRR4302543.429287/1‑94 (MQ=60)
                                                                                             |                                                                                             
AAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTAC  >  NC_000913/3731293‑3731479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑