Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,734,264 2 bp→GC coding (542‑543/825 nt) bax ← putative ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,734,2640CG99.6% 850.6 / NA 264S181C (TCG→TGC) baxputative ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (147/116);  minor base T (0/1);  total (147/117)
Fisher's exact test for biased strand distribution p-value = 4.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
*NC_0009133,734,2650GC99.6% 852.9 / ‑4.6 264S181C (TCG→TGC) baxputative ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (147/116);  minor base A (0/1);  total (147/117)
Fisher's exact test for biased strand distribution p-value = 4.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.81e-01

Only 100 of 266 total aligned reads displayed.

GACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATCGATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATA  >  NC_000913/3734177‑3734351
                                                                                       ||                                                                                      
 aCATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTc                                                                                   <  2:2999209/93‑1 (MQ=255)
  cATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCa                                                                                  <  1:1219722/93‑1 (MQ=255)
  cATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCa                                                                                  <  2:1854548/93‑1 (MQ=255)
  cATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCa                                                                                  <  1:1976914/93‑1 (MQ=255)
  cATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTCGCGCATTGGTACAACGTCCTTTCATGCATTTCa                                                                                  >  1:1177618/1‑93 (MQ=255)
    tAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATg                                                                                >  2:1173383/1‑93 (MQ=255)
     aGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTTGTACAACGTCCTTTCATGCATTTCATGc                                                                               <  2:1390290/93‑1 (MQ=255)
        ctcTCACCGATGCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTGTACCTGGCGCAGTGGTACAACGTCCTTTCATGCATTTCATGCCGa                                                                            <  2:2214044/91‑1 (MQ=255)
        cgcTCACCGATTCTTTGACAGAACTAAGCTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGa                                                                            <  1:1918024/93‑1 (MQ=255)
          cTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAAc                                                                          >  2:843317/1‑93 (MQ=255)
          cTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAAc                                                                          >  1:2856447/1‑93 (MQ=255)
           tCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACa                                                                         <  1:3183449/93‑1 (MQ=255)
           tCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACa                                                                         <  1:458461/93‑1 (MQ=255)
           tCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACa                                                                         <  1:2256454/93‑1 (MQ=255)
           tCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACa                                                                         <  2:1159090/93‑1 (MQ=255)
            cACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGAATTTCATGCCGAACAg                                                                        <  2:836821/93‑1 (MQ=255)
              ccGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgt                                                                      >  1:850149/1‑93 (MQ=255)
              ccGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgt                                                                      <  2:2650469/93‑1 (MQ=255)
               cGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgtt                                                                     <  1:148665/93‑1 (MQ=255)
               cGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgtt                                                                     <  1:4504298/93‑1 (MQ=255)
                gATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttg                                                                    >  2:1545637/1‑93 (MQ=255)
                gATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttg                                                                    >  2:2469965/1‑93 (MQ=255)
                gATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttg                                                                    >  1:2442636/1‑93 (MQ=255)
                gATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttg                                                                    >  1:3642141/1‑93 (MQ=255)
                 aTTCTTTGACAGACCTAAACTGTGAGTTCCCTTTCACTTTTCCTGGCGCATGGGTACAACGTCCTTTCATGCTTTTCATGCCGAACAGgttgt                                                                   <  2:2123499/93‑1 (MQ=255)
                   tCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGTACAGgttgttg                                                                 >  1:1011397/1‑93 (MQ=255)
                    cTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttgt                                                                >  1:3029918/1‑93 (MQ=255)
                     tttGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttgtt                                                               >  2:733344/1‑93 (MQ=255)
                     tttGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttgtt                                                               <  1:2004387/93‑1 (MQ=255)
                     tttGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttgtt                                                               <  1:2567167/93‑1 (MQ=255)
                     tttGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGAGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttgtt                                                               >  1:3296689/1‑93 (MQ=255)
                       tGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttgttgt                                                             <  2:3075507/93‑1 (MQ=255)
                        taCAGAACTAATCTGTGAGTACCCTTTCGCTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttgttgtt                                                            <  1:2322887/92‑1 (MQ=255)
                        gACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttgttgtt                                                            <  2:3482210/93‑1 (MQ=255)
                        gACAAAACTAAACTGTGAGTACCCTATCACGTTACCTGGCGCATTGGTACAACCTCCCTTCATGCATTTCATGCCGAACAGgttgttggtgtt                                                            >  2:4302326/1‑93 (MQ=255)
                         aCAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGttgttgttgttg                                                           >  2:3947392/1‑93 (MQ=255)
                          cAGAACTAAACTGTGATTACCCTGTCACTGTACCTGGCGCATTGGTACAACGTCCTTTCATTCATTTCATTTCGAACAGGTTGTTGTTGTTgc                                                          <  1:3848966/93‑1 (MQ=255)
                          cAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTgc                                                          <  2:2552259/93‑1 (MQ=255)
                            gAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGCACAGGTTGTTGTTGTTgcgc                                                        >  2:4252738/1‑93 (MQ=255)
                            gAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTgcgc                                                        <  2:4059676/93‑1 (MQ=255)
                            gAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTgcgc                                                        >  1:4261332/1‑93 (MQ=255)
                            gAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTgcgc                                                        <  1:4148157/93‑1 (MQ=255)
                              aCTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTgcgcgc                                                      >  2:2861135/1‑93 (MQ=255)
                               cTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGcc                                                     <  2:4669072/93‑1 (MQ=255)
                               cTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGcc                                                     >  2:1744238/1‑93 (MQ=255)
                                tAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCa                                                    <  1:3205935/93‑1 (MQ=255)
                                  aaCTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGc                                                  <  2:3293287/93‑1 (MQ=255)
                                   aCTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCt                                                 <  2:4110079/93‑1 (MQ=255)
                                   aCTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCt                                                 <  2:389108/93‑1 (MQ=255)
                                   aCTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCt                                                 <  1:1030110/93‑1 (MQ=255)
                                    cTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCtt                                                >  2:2916173/1‑93 (MQ=255)
                                         aGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACg                                           >  2:3252101/1‑93 (MQ=255)
                                          gTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGt                                          <  1:3110178/93‑1 (MQ=255)
                                           tACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGtt                                         <  2:1490775/93‑1 (MQ=255)
                                            aCCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTc                                        >  2:4480120/1‑93 (MQ=255)
                                              ccTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTccc                                      >  1:639073/1‑93 (MQ=255)
                                               cTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTcccc                                     <  1:755595/93‑1 (MQ=255)
                                               cTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTcccc                                     >  1:1540997/1‑93 (MQ=255)
                                               cTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTcccc                                     >  1:3409662/1‑93 (MQ=255)
                                               cTTTCACTTTACCTGGAGCATTCGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGATGTTGTTGTAGCGCGCCAGCTTCGACGTTccca                                     >  2:618656/1‑92 (MQ=255)
                                                tttCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCa                                    >  1:1399799/1‑93 (MQ=255)
                                                  tCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAAc                                  >  2:1293545/1‑93 (MQ=255)
                                                   taCTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAAcc                                 <  1:91821/92‑1 (MQ=255)
                                                   cACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAAcc                                 >  1:1788016/1‑93 (MQ=255)
                                                   cACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAAcc                                 <  2:3040517/93‑1 (MQ=255)
                                                   cACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAAcc                                 >  2:3722168/1‑93 (MQ=255)
                                                    aCTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCg                                >  1:2823460/1‑93 (MQ=255)
                                                     cTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGc                               >  1:220975/1‑93 (MQ=255)
                                                      tttACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCt                              <  1:953184/93‑1 (MQ=255)
                                                       ttACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCtt                             >  2:510706/1‑93 (MQ=255)
                                                        tACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCttt                            <  2:670438/93‑1 (MQ=255)
                                                         aCCTGCCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTc                           <  2:2417220/93‑1 (MQ=255)
                                                          ccTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTct                          >  1:1298439/1‑93 (MQ=255)
                                                          ccTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTct                          <  1:2732924/93‑1 (MQ=255)
                                                          ccTGGCGCATTGGGACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTct                          <  2:1446394/93‑1 (MQ=255)
                                                           cTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTctg                         >  2:399614/1‑93 (MQ=255)
                                                           cTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTctg                         >  2:3622362/1‑93 (MQ=255)
                                                                gcATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCa                    >  1:1601206/1‑93 (MQ=255)
                                                                gcATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCa                    <  1:2067143/93‑1 (MQ=255)
                                                                gcATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCa                    <  1:789442/93‑1 (MQ=255)
                                                                 cATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAg                   >  2:4201510/1‑93 (MQ=255)
                                                                 cATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAg                   >  2:888815/1‑93 (MQ=255)
                                                                 cATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAG‑‑‑GTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCa                <  1:4383277/93‑1 (MQ=255)
                                                                   ttGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGcc                 >  1:1942167/1‑93 (MQ=255)
                                                                    tGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCa                >  1:4552516/1‑93 (MQ=255)
                                                                     ggTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAt               >  1:3451737/1‑93 (MQ=255)
                                                                     ggTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAt               <  2:2933262/93‑1 (MQ=255)
                                                                      gTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAtc              >  2:4670862/1‑93 (MQ=255)
                                                                       tACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAtcg             <  2:1027973/93‑1 (MQ=255)
                                                                       tACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAtcg             >  2:3626881/1‑93 (MQ=255)
                                                                       tACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAtcg             >  2:2606194/1‑93 (MQ=255)
                                                                          aaCGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAtcgtcg          >  1:577415/1‑93 (MQ=255)
                                                                           aCGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGc         >  1:675392/1‑93 (MQ=255)
                                                                           aCGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGc         >  2:2055929/1‑93 (MQ=255)
                                                                           aCGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGc         >  1:689756/1‑93 (MQ=255)
                                                                             gTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGccc       >  2:1621307/1‑92 (MQ=255)
                                                                              tCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGccac      >  2:3164177/1‑93 (MQ=255)
                                                                                cTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGccacca    <  1:1837239/93‑1 (MQ=255)
                                                                                cTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGccacca    >  1:4353922/1‑93 (MQ=255)
                                                                                  ttCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATa  <  2:2348448/93‑1 (MQ=255)
                                                                                       ||                                                                                      
GACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATCGATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATA  >  NC_000913/3734177‑3734351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTAGTGACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATCGATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATACT  >  NC_000913/3734171‑3734353
                                                                                             |                                                                                         
GTTAGTGACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATG                                                                                           <  SRR4302535.2602206/94‑1 (MQ=60)
GTTAGTGACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATG                                                                                           <  SRR4302535.1375497/94‑1 (MQ=60)
     TGACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTT                                                                                      <  SRR4302535.2106522/94‑1 (MQ=60)
      GACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTC                                                                                     <  SRR4302535.4065656/94‑1 (MQ=60)
       ACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGAGCATTGGTACAACGTCCTTTCATGCATTTCA                                                                                    >  SRR4302535.321467/1‑94 (MQ=60)
       ACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTCGCGCATTGGTACAACGTCCTTTCATGCATTTCA                                                                                    >  SRR4302535.1186021/1‑94 (MQ=60)
        CATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCAT                                                                                   <  SRR4302535.693390/94‑1 (MQ=60)
        CATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCAT                                                                                   <  SRR4302535.1991152/94‑1 (MQ=60)
        CATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCAT                                                                                   <  SRR4302535.4671541/94‑1 (MQ=60)
        CATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCAT                                                                                   >  SRR4302535.4559793/1‑94 (MQ=60)
        CATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCAT                                                                                   <  SRR4302535.1421993/94‑1 (MQ=60)
        CATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCAT                                                                                   <  SRR4302535.1228469/94‑1 (MQ=60)
         ATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATG                                                                                  >  SRR4302535.4283496/1‑94 (MQ=60)
           AGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCC                                                                                <  SRR4302535.864233/94‑1 (MQ=60)
            GGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCG                                                                               <  SRR4302535.956593/94‑1 (MQ=60)
              CGCTCACCGATTCTTTGACAGAACTAAGCTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAA                                                                             <  SRR4302535.1931846/94‑1 (MQ=60)
              CGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAA                                                                             >  SRR4302535.4532012/1‑94 (MQ=60)
               GCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAAC                                                                            >  SRR4302535.2876687/1‑94 (MQ=60)
                CTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACA                                                                           <  SRR4302535.1656024/94‑1 (MQ=60)
                 TCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAG                                                                          <  SRR4302535.3205442/94‑1 (MQ=60)
                 TCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAG                                                                          <  SRR4302535.3164036/94‑1 (MQ=60)
                 TCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAG                                                                          <  SRR4302535.2272517/94‑1 (MQ=60)
                 TCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAG                                                                          <  SRR4302535.461669/94‑1 (MQ=60)
                 TCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAG                                                                          <  SRR4302535.834173/94‑1 (MQ=60)
                   ACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGT                                                                        >  SRR4302535.856136/1‑94 (MQ=60)
                    CCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTT                                                                       <  SRR4302535.3714666/94‑1 (MQ=60)
                     CGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTG                                                                      >  SRR4302535.2460233/1‑94 (MQ=60)
                     CGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTG                                                                      <  SRR4302535.861103/94‑1 (MQ=60)
                     CGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTG                                                                      <  SRR4302535.2277019/94‑1 (MQ=60)
                     CGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTG                                                                      <  SRR4302535.4535225/94‑1 (MQ=60)
                     CGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTG                                                                      >  SRR4302535.592818/1‑94 (MQ=60)
                     CGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTG                                                                      <  SRR4302535.149701/94‑1 (MQ=60)
                     CGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTG                                                                      >  SRR4302535.3667366/1‑94 (MQ=60)
                      GATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGT                                                                     >  SRR4302535.3272508/1‑94 (MQ=60)
                      GATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGT                                                                     <  SRR4302535.4570477/94‑1 (MQ=60)
                        TTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGTACAGGTTGTTG                                                                   >  SRR4302535.1018582/1‑94 (MQ=60)
                         TCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGT                                                                  >  SRR4302535.714225/1‑94 (MQ=60)
                         TCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGT                                                                  >  SRR4302535.3051128/1‑94 (MQ=60)
                          CTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTT                                                                 >  SRR4302535.2595496/1‑94 (MQ=60)
                          CTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTT                                                                 <  SRR4302535.75670/94‑1 (MQ=60)
                          CTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTT                                                                 >  SRR4302535.4353341/1‑94 (MQ=60)
                          CTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGAGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTT                                                                 >  SRR4302535.3319608/1‑94 (MQ=60)
                           TTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTG                                                                <  SRR4302535.2585636/94‑1 (MQ=60)
                           TTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTG                                                                <  SRR4302535.2018830/94‑1 (MQ=60)
                             TGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTT                                                              <  SRR4302535.1409540/94‑1 (MQ=60)
                              TACAGAACTAATCTGTGAGTACCCTTTCGCTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTG                                                             <  SRR4302535.2339509/94‑1 (MQ=60)
                              GACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTG                                                             >  SRR4302535.672691/1‑94 (MQ=60)
                              GACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTG                                                             <  SRR4302535.1963279/94‑1 (MQ=60)
                              GACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTG                                                             <  SRR4302535.4363424/94‑1 (MQ=60)
                              GACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTG                                                             >  SRR4302535.833913/1‑94 (MQ=60)
                              GACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTG                                                             <  SRR4302535.3327456/94‑1 (MQ=60)
                               ACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGC                                                            >  SRR4302535.3197732/1‑94 (MQ=60)
                                CAGAACTAAACTGTGATTACCCTGTCACTGTACCTGGCGCATTGGTACAACGTCCTTTCATTCATTTCATTTCGAACAGGTTGTTGTTGTTGCG                                                           <  SRR4302535.3875456/94‑1 (MQ=60)
                                CAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGAGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCG                                                           >  SRR4302535.694208/1‑94 (MQ=60)
                                 AGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGC                                                          >  SRR4302535.4290518/1‑94 (MQ=60)
                                 AGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGC                                                          >  SRR4302535.155497/1‑94 (MQ=60)
                                  GAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCG                                                         <  SRR4302535.4176558/94‑1 (MQ=60)
                                  GAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCG                                                         <  SRR4302535.1544958/94‑1 (MQ=60)
                                  GAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCG                                                         >  SRR4302535.2852801/1‑94 (MQ=60)
                                    ACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCC                                                       >  SRR4302535.4422581/1‑94 (MQ=60)
                                      TAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAG                                                     <  SRR4302535.3228137/94‑1 (MQ=60)
                                      TAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAG                                                     <  SRR4302535.3357565/94‑1 (MQ=60)
                                       AAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGC                                                    >  SRR4302535.968520/1‑94 (MQ=60)
                                        AACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCT                                                   >  SRR4302535.4659178/1‑94 (MQ=60)
                                         ACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTT                                                  >  SRR4302535.2397433/1‑94 (MQ=60)
                                         ACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTT                                                  <  SRR4302535.1037419/94‑1 (MQ=60)
                                         ACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTT                                                  <  SRR4302535.1948099/94‑1 (MQ=60)
                                           TGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCG                                                >  SRR4302535.124773/1‑94 (MQ=60)
                                             TGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGAC                                              <  SRR4302535.2022967/94‑1 (MQ=60)
                                             TGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGAC                                              <  SRR4302535.2003633/94‑1 (MQ=60)
                                               AGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGT                                            >  SRR4302535.223862/1‑94 (MQ=60)
                                                GTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTT                                           <  SRR4302535.3131831/94‑1 (MQ=60)
                                                GTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTT                                           <  SRR4302535.1991721/94‑1 (MQ=60)
                                                  ACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCC                                         >  SRR4302535.2199252/1‑94 (MQ=60)
                                                   CCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCC                                        >  SRR4302535.643540/1‑94 (MQ=60)
                                                   CCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCC                                        >  SRR4302535.2836020/1‑94 (MQ=60)
                                                   CCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGACAGCTTCGACGTTGCC                                        >  SRR4302535.152328/1‑94 (MQ=60)
                                                    CCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCC                                       >  SRR4302535.3433389/1‑94 (MQ=60)
                                                    CCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCC                                       >  SRR4302535.1552109/1‑94 (MQ=60)
                                                     CTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCA                                      >  SRR4302535.1409795/1‑94 (MQ=60)
                                                     CTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCA                                      >  SRR4302535.397019/1‑94 (MQ=60)
                                                     CTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCA                                      >  SRR4302535.4596657/1‑94 (MQ=60)
                                                     CTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCA                                      <  SRR4302535.4241803/94‑1 (MQ=60)
                                                     CTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCA                                      <  SRR4302535.760917/94‑1 (MQ=60)
                                                      TTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAC                                     >  SRR4302535.3033379/1‑94 (MQ=60)
                                                      TTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAC                                     >  SRR4302535.3445831/1‑94 (MQ=60)
                                                      TTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAA                                     <  SRR4302535.1825464/94‑1 (MQ=60)
                                                        TCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACC                                   >  SRR4302535.1800952/1‑94 (MQ=60)
                                                         TACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCG                                  <  SRR4302535.92469/94‑1 (MQ=60)
                                                         CACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCG                                  >  SRR4302535.1398527/1‑94 (MQ=60)
                                                         CACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCG                                  >  SRR4302535.2843501/1‑94 (MQ=60)
                                                          ACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGC                                 >  SRR4302535.222499/1‑94 (MQ=60)
                                                           CTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCT                                <  SRR4302535.1897215/94‑1 (MQ=60)
                                                            TTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTT                               <  SRR4302535.959939/94‑1 (MQ=60)
                                                             TTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTT                              <  SRR4302535.2836021/94‑1 (MQ=60)
                                                               ACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCT                            >  SRR4302535.1035661/1‑94 (MQ=60)
                                                               ACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCT                            >  SRR4302535.1307699/1‑94 (MQ=60)
                                                                CCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTG                           <  SRR4302535.583498/94‑1 (MQ=60)
                                                                CCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTG                           >  SRR4302535.2581512/1‑94 (MQ=60)
                                                                CCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTG                           <  SRR4302535.2752430/94‑1 (MQ=60)
                                                                     CGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCA                      <  SRR4302535.1135460/94‑1 (MQ=60)
                                                                     CGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCA                      >  SRR4302535.1612755/1‑94 (MQ=60)
                                                                      GCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAG                     <  SRR4302535.794983/94‑1 (MQ=60)
                                                                      GCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAG                     <  SRR4302535.2082045/94‑1 (MQ=60)
                                                                       CATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAG‑‑‑GTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAT                 <  SRR4302535.4413265/94‑1 (MQ=60)
                                                                        ATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCC                   >  SRR4302535.3486385/1‑94 (MQ=60)
                                                                        ATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCC                   <  SRR4302535.764041/94‑1 (MQ=60)
                                                                        ATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCC                   >  SRR4302535.1956156/1‑94 (MQ=60)
                                                                         TTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCA                  >  SRR4302535.4583625/1‑94 (MQ=60)
                                                                          TGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAT                 >  SRR4302535.3475729/1‑94 (MQ=60)
                                                                          TGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAT                 <  SRR4302535.2211842/94‑1 (MQ=60)
                                                                            GTACAACGNCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCG               >  SRR4302535.2866683/1‑94 (MQ=60)
                                                                             TACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGT              >  SRR4302535.1984199/1‑94 (MQ=60)
                                                                             TACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGT              >  SRR4302535.3107389/1‑94 (MQ=60)
                                                                             TACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGT              >  SRR4302535.2510115/1‑94 (MQ=60)
                                                                              ACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTC             <  SRR4302535.1220213/94‑1 (MQ=60)
                                                                               CAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCG            >  SRR4302535.1864705/1‑94 (MQ=60)
                                                                               CAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCG            <  SRR4302535.2238562/94‑1 (MQ=60)
                                                                               CAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCG            >  SRR4302535.581470/1‑94 (MQ=60)
                                                                               CAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCG            >  SRR4302535.4410768/1‑94 (MQ=60)
                                                                                AACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGC           <  SRR4302535.3104313/94‑1 (MQ=60)
                                                                                AACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGC           >  SRR4302535.680112/1‑94 (MQ=60)
                                                                                AACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGC           >  SRR4302535.694589/1‑94 (MQ=60)
                                                                                 ACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCC          >  SRR4302535.2384999/1‑94 (MQ=60)
                                                                                 ACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCC          >  SRR4302535.1263255/1‑94 (MQ=60)
                                                                                  CGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCC         >  SRR4302535.1803076/1‑94 (MQ=60)
                                                                                     CCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGGCACCA      >  SRR4302535.3744675/1‑94 (MQ=60)
                                                                                     CCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCA      >  SRR4302535.104293/1‑94 (MQ=60)
                                                                                     CCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCA      >  SRR4302535.4383721/1‑94 (MQ=60)
                                                                                      CTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCAT     <  SRR4302535.1850493/94‑1 (MQ=60)
                                                                                      CTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCAT     <  SRR4302535.1553752/94‑1 (MQ=60)
                                                                                      CTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCAT     >  SRR4302535.343190/1‑94 (MQ=60)
                                                                                      CTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCAT     <  SRR4302535.3510280/94‑1 (MQ=60)
                                                                                       TTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATA    <  SRR4302535.3282592/94‑1 (MQ=60)
                                                                                         TCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCACCCGCTTTCTGCTGCAGCCATCGTCGCCACCATACT  <  SRR4302535.3571091/94‑1 (MQ=60)
                                                                                         TCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATACT  <  SRR4302535.714196/94‑1 (MQ=60)
                                                                                         TCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATACT  <  SRR4302535.495524/94‑1 (MQ=60)
                                                                                         TCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATACT  <  SRR4302535.1763588/94‑1 (MQ=60)
                                                                                         TCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATACT  <  SRR4302535.4515522/94‑1 (MQ=60)
                                                                                         TCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATACT  >  SRR4302535.1551991/1‑94 (MQ=60)
                                                                                         TCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATACT  >  SRR4302535.3667533/1‑94 (MQ=60)
                                                                                             |                                                                                         
GTTAGTGACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATCGATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATACT  >  NC_000913/3734171‑3734353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑