Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,806,424 G→A T86T (ACG→ACA)  kdtA → 3‑deoxy‑D‑manno‑octulosonic‑acid transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,806,4240GA99.2% 906.0 / ‑4.3 267T86T (ACG→ACA) kdtA3‑deoxy‑D‑manno‑octulosonic‑acid transferase
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base A (127/137);  total (127/140)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.05e-01

Only 100 of 275 total aligned reads displayed.

TCCGTCGGTGAAACTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCG  >  NC_000913/3806335‑3806513
                                                                                         |                                                                                         
tccgtcGGTGAAACTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCa                                                                                        <  2:2577747/93‑1 (MQ=255)
 ccgtcGGTGAAACTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCaa                                                                                       <  1:1369947/93‑1 (MQ=255)
  cgtcGGTGAAACTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAAc                                                                                      >  2:1293489/1‑93 (MQ=255)
  cgtcGGTGAAACTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAAc                                                                                      <  2:2999902/93‑1 (MQ=255)
   gtcGGTGAAACTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAAcc                                                                                     >  1:3561912/1‑93 (MQ=255)
      ggTGAAACTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGt                                                                                  >  2:84275/1‑93 (MQ=255)
       gTGAAACTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGtt                                                                                 <  1:917174/93‑1 (MQ=255)
       gTGAAACTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGtt                                                                                 <  2:2971105/93‑1 (MQ=255)
        tGAAGCTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTc                                                                                <  1:3488449/93‑1 (MQ=255)
        tGAAACTCTGGCGGCAATCCCGTTGGTTCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTc                                                                                <  2:3129056/93‑1 (MQ=255)
          aaaCTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCgg                                                                              <  2:4460077/93‑1 (MQ=255)
           aaCTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGa                                                                             <  1:103626/93‑1 (MQ=255)
           aaCTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGa                                                                             <  2:3502613/93‑1 (MQ=255)
              tctGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAgcg                                                                          >  1:2365582/1‑93 (MQ=255)
                  gcggcAATCCGGTTGGTGCGCGAGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACATCCATGACACCAACCGGTTCGGAGCGCGTa                                                                      <  2:1530405/93‑1 (MQ=255)
                  gcggcAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTa                                                                      <  1:3378858/93‑1 (MQ=255)
                  gcggcAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTa                                                                      <  2:2277932/93‑1 (MQ=255)
                   cggcAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTAc                                                                     <  1:4240356/93‑1 (MQ=255)
                   cggcAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTAc                                                                     <  1:2291895/93‑1 (MQ=255)
                       aaTCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGCCACCAACCGGTTCGGAGCGCGTACAATc                                                                 <  2:2256402/93‑1 (MQ=255)
                       aaTCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATc                                                                 <  2:2546321/93‑1 (MQ=255)
                       aaTCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATc                                                                 <  1:1443753/93‑1 (MQ=255)
                       aaTCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATc                                                                 <  2:1356623/93‑1 (MQ=255)
                       aaTCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATc                                                                 >  1:2679306/1‑93 (MQ=255)
                        aTCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCg                                                                >  1:2867864/1‑93 (MQ=255)
                         tCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCgg                                                               >  1:4295126/1‑93 (MQ=255)
                          cccGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGc                                                              >  1:2547141/1‑93 (MQ=255)
                          cccGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGc                                                              <  2:4030105/93‑1 (MQ=255)
                            cGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCtt                                                            >  2:239242/1‑93 (MQ=255)
                            cGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCtt                                                            >  2:230385/1‑93 (MQ=255)
                             gTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCttt                                                           <  2:1556036/93‑1 (MQ=255)
                              ttGGTGCGCGCGCTGCGTCATCGTTATCCTGGTTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGCACACTCGGCCTTc                                                          >  2:517395/1‑93 (MQ=255)
                              ttGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTc                                                          <  2:4655415/93‑1 (MQ=255)
                              ttGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTc                                                          >  1:1195923/1‑93 (MQ=255)
                              ttGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTc                                                          >  1:1727570/1‑93 (MQ=255)
                               tGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCg                                                         <  2:2412955/93‑1 (MQ=255)
                                ggTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCgg                                                        <  1:2826079/93‑1 (MQ=255)
                                  tGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGa                                                      >  2:2071375/1‑93 (MQ=255)
                                  tGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGa                                                      >  2:4288688/1‑93 (MQ=255)
                                   gcgcgcgcTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGaa                                                     <  2:823619/93‑1 (MQ=255)
                                    ggcgcggTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAg                                                    <  1:3097295/92‑1 (MQ=255)
                                    cgcgcgcTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAg                                                    <  2:3823066/93‑1 (MQ=255)
                                      ctcgcgGCGTCATCGTTATCCTGAGTTACCGATTACCGTAACAACCATGACACCAGCCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGa                                                  <  2:3436824/91‑1 (MQ=255)
                                       gcgcTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGAt                                                 <  1:46043/93‑1 (MQ=255)
                                         gcTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGt                                               <  1:4130636/93‑1 (MQ=255)
                                         gcTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGt                                               <  1:1527241/93‑1 (MQ=255)
                                          cTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGtt                                              <  2:2044417/93‑1 (MQ=255)
                                          cTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGtt                                              >  2:3592764/1‑93 (MQ=255)
                                           tGCGTCATCGTTATCCTGATTTACCGATTCCCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTc                                             <  1:3786841/93‑1 (MQ=255)
                                           tGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTc                                             >  1:1333788/1‑93 (MQ=255)
                                            gCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCa                                            >  1:2893844/1‑93 (MQ=255)
                                            gCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCa                                            >  1:4435958/1‑93 (MQ=255)
                                             cGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAg                                           <  1:4048175/93‑1 (MQ=255)
                                                cATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCAc                                        <  2:220098/93‑1 (MQ=255)
                                                 aTCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACg                                       >  1:551385/1‑93 (MQ=255)
                                                  tCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGt                                      >  1:204278/1‑93 (MQ=255)
                                                  tCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGt                                      >  1:2984762/1‑93 (MQ=255)
                                                   cGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGtt                                     >  2:1067884/1‑93 (MQ=255)
                                                   cGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGtt                                     <  1:2471457/93‑1 (MQ=255)
                                                    gTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGttt                                    >  1:3268334/1‑93 (MQ=255)
                                                      tATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTAt                                  <  1:1640460/93‑1 (MQ=255)
                                                       aTCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATc                                 <  1:3015925/93‑1 (MQ=255)
                                                       aTCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATc                                 >  1:2593064/1‑93 (MQ=255)
                                                        tCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCt                                <  1:3154715/93‑1 (MQ=255)
                                                        tCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCt                                >  2:4004859/1‑93 (MQ=255)
                                                        tCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCt                                >  2:1203937/1‑93 (MQ=255)
                                                        tCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCt                                >  1:1022963/1‑93 (MQ=255)
                                                         ccTTATTTACCGATGACCGTAACACCCATGCCACCACCCGTTCCGGAGCGCGTACAATCGGCTTGCGGGAAGGATGTTCAGCACGTTTATCTg                               <  2:504780/93‑1 (MQ=255)
                                                          cTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGc                              >  1:4594348/1‑93 (MQ=255)
                                                           tGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGcc                             >  1:1236847/1‑93 (MQ=255)
                                                           tGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGcc                             >  2:1855570/1‑93 (MQ=255)
                                                            gATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTTCCg                            >  1:38358/1‑93 (MQ=255)
                                                             aTTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGt                           >  1:2735738/1‑93 (MQ=255)
                                                              tttACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTa                          >  2:616918/1‑93 (MQ=255)
                                                               ttACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTAt                         <  1:4352930/93‑1 (MQ=255)
                                                                tACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATg                        <  1:1271840/93‑1 (MQ=255)
                                                                 aCCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGa                       >  2:3295739/1‑93 (MQ=255)
                                                                   cGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATc                     >  2:2727348/1‑93 (MQ=255)
                                                                   cGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATc                     <  1:2350819/93‑1 (MQ=255)
                                                                    gATTACCGTAACACCCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCt                    <  1:4184358/93‑1 (MQ=255)
                                                                    gATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCt                    <  1:1113291/93‑1 (MQ=255)
                                                                      ttACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGc                  <  1:3973587/93‑1 (MQ=255)
                                                                       tACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTggc                 >  1:1778895/1‑91 (MQ=255)
                                                                        aCCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGccc                >  2:393019/1‑93 (MQ=255)
                                                                         ccGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCg               <  2:1777287/93‑1 (MQ=255)
                                                                          cGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGa              <  1:1675744/93‑1 (MQ=255)
                                                                           gTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGAt             >  1:2390455/1‑93 (MQ=255)
                                                                           gTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGAt             >  2:115691/1‑93 (MQ=255)
                                                                            tAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATg            >  2:701040/1‑93 (MQ=255)
                                                                              acaacCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCa          <  2:2762181/93‑1 (MQ=255)
                                                                               caacCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCAc         <  2:761052/93‑1 (MQ=255)
                                                                               caacCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCAc         >  2:880118/1‑93 (MQ=255)
                                                                               caacCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCAc         <  2:369815/93‑1 (MQ=255)
                                                                                aacCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACt        >  1:725332/1‑93 (MQ=255)
                                                                                aacCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACt        >  1:4627076/1‑93 (MQ=255)
                                                                                 acCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTc       >  1:328769/1‑93 (MQ=255)
                                                                                  ccATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCa      >  1:817392/1‑93 (MQ=255)
                                                                                  ccATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCa      >  1:3413906/1‑93 (MQ=255)
                                                                                     tGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAAcc   >  2:4395641/1‑93 (MQ=255)
                                                                                     tGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAAcc   >  1:3049534/1‑93 (MQ=255)
                                                                                         |                                                                                         
TCCGTCGGTGAAACTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCG  >  NC_000913/3806335‑3806513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGTCTCCGTCGGTGAAACTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTC  >  NC_000913/3806331‑3806517
                                                                                             |                                                                                             
CGTCTCCGTCGGTGAAACTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACC                                                                                               >  SRR4302535.4195354/1‑94 (MQ=60)
CGTCTCCGTCGGTGAAACTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACA                                                                                               >  SRR4302535.2772839/1‑94 (MQ=60)
 GTCTCCGTCGGTGAAACTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACAC                                                                                              >  SRR4302535.1038518/1‑94 (MQ=60)
 GTCTCCGTCGGTGAAACTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACAC                                                                                              >  SRR4302535.4474177/1‑94 (MQ=60)
 GTCTCCGTCGGTGAAACTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACAC                                                                                              <  SRR4302535.3093668/94‑1 (MQ=60)
  TCTCCGTCGGTGAAACTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACC                                                                                             <  SRR4302535.3556833/94‑1 (MQ=60)
   CTCCGTCGGTGAAACTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCA                                                                                            >  SRR4302535.4431590/1‑94 (MQ=60)
   CTCCGTCGGTGAAACTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCA                                                                                            >  SRR4302535.4431589/1‑94 (MQ=60)
   CTCCGTCGGTGAAACTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGCTATCCTGATTTACCGATTACCGTAACAACCATGACACCA                                                                                            >  SRR4302535.3017413/1‑94 (MQ=60)
     CCGTCGGTGAAACTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAAC                                                                                          <  SRR4302535.1379735/94‑1 (MQ=60)
     CCGTCGGTGAAACTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAAC                                                                                          <  SRR4302535.1754255/94‑1 (MQ=60)
     CCGTCGGTGAAACTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGGTATCCTGATTTACCGATTACCGTAACAACCATGACACCAAC                                                                                          >  SRR4302535.2775808/1‑94 (MQ=60)
      CGTCGGTGAAACTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACC                                                                                         <  SRR4302535.2903268/94‑1 (MQ=60)
      CGTCGGTGAAACTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACC                                                                                         >  SRR4302535.3586612/1‑94 (MQ=60)
       GTCGGTGAAACTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTGCCGATTACCGTAACAACCATGACACCAACCG                                                                                        <  SRR4302535.23251/94‑1 (MQ=60)
         CGGTGAAACTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGT                                                                                      >  SRR4302535.3156725/1‑94 (MQ=60)
           GTGAAACTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTG                                                                                    >  SRR4302535.3299252/1‑94 (MQ=60)
           GTGAAACTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTC                                                                                    >  SRR4302535.2681575/1‑94 (MQ=60)
           GTGAAACTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTC                                                                                    <  SRR4302535.923681/94‑1 (MQ=60)
            TGAAGCTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCG                                                                                   <  SRR4302535.3512668/94‑1 (MQ=60)
            TGAAACTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCG                                                                                   <  SRR4302535.2705676/94‑1 (MQ=60)
            TGAAACTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCG                                                                                   >  SRR4302535.3398495/1‑94 (MQ=60)
             GAAACTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGG                                                                                  <  SRR4302535.4255800/94‑1 (MQ=60)
              AAACTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACTGGTTCGGA                                                                                 >  SRR4302535.4633647/1‑94 (MQ=60)
              AAACTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGA                                                                                 <  SRR4302535.233478/94‑1 (MQ=60)
              AAACTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGA                                                                                 <  SRR4302535.2058592/94‑1 (MQ=60)
               AACTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGTAG                                                                                >  SRR4302535.3485347/1‑94 (MQ=60)
               AACTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAG                                                                                <  SRR4302535.104359/94‑1 (MQ=60)
                 CTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCG                                                                              <  SRR4302535.1427125/94‑1 (MQ=60)
                 CTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCG                                                                              >  SRR4302535.2382564/1‑94 (MQ=60)
                 CTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCG                                                                              >  SRR4302535.883457/1‑94 (MQ=60)
                  TCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGC                                                                             <  SRR4302535.1411933/94‑1 (MQ=60)
                      GCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTAC                                                                         <  SRR4302535.3402351/94‑1 (MQ=60)
                      GCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTAC                                                                         <  SRR4302535.1714357/94‑1 (MQ=60)
                       CGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACA                                                                        <  SRR4302535.2308289/94‑1 (MQ=60)
                       CGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACA                                                                        <  SRR4302535.4081933/94‑1 (MQ=60)
                       CGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACA                                                                        <  SRR4302535.4269379/94‑1 (MQ=60)
                         GCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAAT                                                                      <  SRR4302535.501668/94‑1 (MQ=60)
                          CAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATC                                                                     >  SRR4302535.2698474/1‑94 (MQ=60)
                          CAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATC                                                                     >  SRR4302535.573875/1‑94 (MQ=60)
                          CAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATC                                                                     <  SRR4302535.158511/94‑1 (MQ=60)
                           AATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCG                                                                    >  SRR4302535.4177478/1‑94 (MQ=60)
                           AATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCG                                                                    >  SRR4302535.2888175/1‑94 (MQ=60)
                           AATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCG                                                                    >  SRR4302535.3125728/1‑94 (MQ=60)
                           AATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCG                                                                    <  SRR4302535.1454077/94‑1 (MQ=60)
                           AATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCG                                                                    >  SRR4302535.3472162/1‑94 (MQ=60)
                            ATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGG                                                                   >  SRR4302535.4324515/1‑94 (MQ=60)
                            ATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGG                                                                   >  SRR4302535.1355581/1‑94 (MQ=60)
                            ATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGG                                                                   >  SRR4302535.454153/1‑94 (MQ=60)
                            ATCCCGTTGGTCCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGG                                                                   <  SRR4302535.1675239/94‑1 (MQ=60)
                            ATCCCGTTGGGGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGG                                                                   <  SRR4302535.4366081/94‑1 (MQ=60)
                             TCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGC                                                                  >  SRR4302535.2565467/1‑94 (MQ=60)
                               CCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTT                                                                >  SRR4302535.130080/1‑94 (MQ=60)
                               CCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTT                                                                <  SRR4302535.1083829/94‑1 (MQ=60)
                                CGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTT                                                               <  SRR4302535.3383686/94‑1 (MQ=60)
                                 GTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTC                                                              >  SRR4302535.1204491/1‑94 (MQ=60)
                                 GTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTC                                                              >  SRR4302535.3087744/1‑94 (MQ=60)
                                 GTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTC                                                              >  SRR4302535.1740058/1‑94 (MQ=60)
                                  TTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCG                                                             <  SRR4302535.117081/94‑1 (MQ=60)
                                   TGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGG                                                            <  SRR4302535.2897700/94‑1 (MQ=60)
                                   TGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGG                                                            <  SRR4302535.1363311/94‑1 (MQ=60)
                                    GGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGG                                                           >  SRR4302535.1670226/1‑94 (MQ=60)
                                    GGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGG                                                           <  SRR4302535.2846139/94‑1 (MQ=60)
                                       GCGCGCTCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACACCCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAG                                                        <  SRR4302535.1627802/94‑1 (MQ=60)
                                       GCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAG                                                        <  SRR4302535.4271298/94‑1 (MQ=60)
                                       GCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAG                                                        <  SRR4302535.3975486/94‑1 (MQ=60)
                                        GGCGCGGTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGG                                                       <  SRR4302535.3118862/94‑1 (MQ=60)
                                        CGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGG                                                       >  SRR4302535.2735076/1‑94 (MQ=60)
                                        CGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGG                                                       <  SRR4302535.1506691/94‑1 (MQ=60)
                                         GCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGA                                                      <  SRR4302535.1839909/94‑1 (MQ=60)
                                          CGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGAT                                                     <  SRR4302535.2653032/94‑1 (MQ=60)
                                          CGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGAT                                                     <  SRR4302535.1924552/94‑1 (MQ=60)
                                           GCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATG                                                    <  SRR4302535.46392/94‑1 (MQ=60)
                                           GCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATG                                                    <  SRR4302535.4655675/94‑1 (MQ=60)
                                            CGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACCCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGT                                                   <  SRR4302535.1135007/94‑1 (MQ=60)
                                            CGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGT                                                   >  SRR4302535.3403051/1‑94 (MQ=60)
                                            CGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGT                                                   >  SRR4302535.2392313/1‑94 (MQ=60)
                                            CGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGT                                                   >  SRR4302535.3262207/1‑94 (MQ=60)
                                            CGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGT                                                   >  SRR4302535.1319730/1‑94 (MQ=60)
                                             GCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTT                                                  <  SRR4302535.4158897/94‑1 (MQ=60)
                                             GCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTT                                                  <  SRR4302535.1538244/94‑1 (MQ=60)
                                             GCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTT                                                  >  SRR4302535.3535403/1‑94 (MQ=60)
                                              CTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTC                                                 >  SRR4302535.1112689/1‑94 (MQ=60)
                                              CTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTC                                                 >  SRR4302535.1343301/1‑94 (MQ=60)
                                               TGCGTCATCGTTATCCTGATTTACCGATTCCCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCA                                                <  SRR4302535.3812963/94‑1 (MQ=60)
                                               TGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCA                                                >  SRR4302535.4466361/1‑94 (MQ=60)
                                               TGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCA                                                >  SRR4302535.4267865/1‑94 (MQ=60)
                                               TGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCA                                                >  SRR4302535.2914313/1‑94 (MQ=60)
                                                GCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAG                                               <  SRR4302535.4596813/94‑1 (MQ=60)
                                                GCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAG                                               <  SRR4302535.2932304/94‑1 (MQ=60)
                                                 CGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGC                                              <  SRR4302535.4075885/94‑1 (MQ=60)
                                                 CGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGC                                              >  SRR4302535.4642156/1‑94 (MQ=60)
                                                   TCNTNGNTNTCCTGATTTACCGNTTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCAC                                            <  SRR4302535.3407547/94‑1 (MQ=60)
                                                    CATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACG                                           >  SRR4302535.555245/1‑94 (MQ=60)
                                                     ATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGT                                          >  SRR4302535.526768/1‑94 (MQ=60)
                                                     ATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGT                                          >  SRR4302535.205677/1‑94 (MQ=60)
                                                     ATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGT                                          >  SRR4302535.3005698/1‑94 (MQ=60)
                                                      TCGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTT                                         <  SRR4302535.3050729/94‑1 (MQ=60)
                                                       CGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTT                                        >  SRR4302535.121695/1‑94 (MQ=60)
                                                       CGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTT                                        >  SRR4302535.2401376/1‑94 (MQ=60)
                                                       CGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTT                                        <  SRR4302535.2963603/94‑1 (MQ=60)
                                                       CGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTT                                        >  SRR4302535.3291039/1‑94 (MQ=60)
                                                       CGTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTT                                        <  SRR4302535.2489273/94‑1 (MQ=60)
                                                        GTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTA                                       >  SRR4302535.3687386/1‑94 (MQ=60)
                                                        GTTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTA                                       >  SRR4302535.3841772/1‑94 (MQ=60)
                                                         TTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTAT                                      <  SRR4302535.104295/94‑1 (MQ=60)
                                                         TTATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTAT                                      <  SRR4302535.384770/94‑1 (MQ=60)
                                                         TTATCCTGATTTACCGACTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTAT                                      <  SRR4302535.1723550/94‑1 (MQ=60)
                                                          TATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATC                                     <  SRR4302535.1652319/94‑1 (MQ=60)
                                                          TATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATC                                     <  SRR4302535.3099641/94‑1 (MQ=60)
                                                          TATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATC                                     >  SRR4302535.2611693/1‑94 (MQ=60)
                                                           ATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCT                                    <  SRR4302535.398293/94‑1 (MQ=60)
                                                           ATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCT                                    >  SRR4302535.1030228/1‑94 (MQ=60)
                                                           ATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCT                                    <  SRR4302535.3037058/94‑1 (MQ=60)
                                                           ATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCT                                    <  SRR4302535.2182975/94‑1 (MQ=60)
                                                           ATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCT                                    >  SRR4302535.3008972/1‑94 (MQ=60)
                                                           ATCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCT                                    <  SRR4302535.1267747/94‑1 (MQ=60)
                                                            TCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTG                                   <  SRR4302535.3775779/94‑1 (MQ=60)
                                                            TCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTG                                   <  SRR4302535.3176629/94‑1 (MQ=60)
                                                            TCCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTG                                   <  SRR4302535.2376967/94‑1 (MQ=60)
                                                             CCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGC                                  <  SRR4302535.2844932/94‑1 (MQ=60)
                                                             CCTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGC                                  >  SRR4302535.4625613/1‑94 (MQ=60)
                                                              CTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCC                                 >  SRR4302535.1245681/1‑94 (MQ=60)
                                                              CTGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCC                                 >  SRR4302535.4194264/1‑94 (MQ=60)
                                                               TGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTTCCG                                >  SRR4302535.38654/1‑94 (MQ=60)
                                                               TGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCG                                >  SRR4302535.2513995/1‑94 (MQ=60)
                                                               TGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCG                                <  SRR4302535.875224/94‑1 (MQ=60)
                                                               TGATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCG                                <  SRR4302535.435830/94‑1 (MQ=60)
                                                                GATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGT                               >  SRR4302535.2782986/1‑94 (MQ=60)
                                                                GATTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGT                               >  SRR4302535.2755257/1‑94 (MQ=60)
                                                                  TTTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTAT                             >  SRR4302535.2847523/1‑94 (MQ=60)
                                                                   TTACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATG                            <  SRR4302535.4382720/94‑1 (MQ=60)
                                                                    TACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGA                           <  SRR4302535.1280929/94‑1 (MQ=60)
                                                                    TACCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGA                           <  SRR4302535.710090/94‑1 (MQ=60)
                                                                      CCGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATC                         <  SRR4302535.4056076/94‑1 (MQ=60)
                                                                       CGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCT                        <  SRR4302535.2367673/94‑1 (MQ=60)
                                                                       CGATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCT                        <  SRR4302535.241428/94‑1 (MQ=60)
                                                                        GATTACCGTAACACCCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTG                       <  SRR4302535.4212987/94‑1 (MQ=60)
                                                                        GATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTG                       <  SRR4302535.1121231/94‑1 (MQ=60)
                                                                         ATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGC                      >  SRR4302535.762918/1‑94 (MQ=60)
                                                                         ATTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGC                      >  SRR4302535.1219458/1‑94 (MQ=60)
                                                                          TTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGGC                     >  SRR4302535.1791775/1‑94 (MQ=60)
                                                                          TTACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCC                     <  SRR4302535.4000729/94‑1 (MQ=60)
                                                                           TACCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCC                    <  SRR4302535.3220262/94‑1 (MQ=60)
                                                                             CCGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGA                  >  SRR4302535.4086734/1‑94 (MQ=60)
                                                                              CGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGAT                 >  SRR4302535.3421068/1‑94 (MQ=60)
                                                                              CGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGAT                 >  SRR4302535.2407643/1‑94 (MQ=60)
                                                                              CGTAACAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGAT                 <  SRR4302535.1687878/94‑1 (MQ=60)
                                                                                   CAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACT            >  SRR4302535.4658488/1‑94 (MQ=60)
                                                                                   CAACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACT            >  SRR4302535.730422/1‑94 (MQ=60)
                                                                                    AACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTC           >  SRR4302535.331050/1‑94 (MQ=60)
                                                                                    AACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTC           >  SRR4302535.2508593/1‑94 (MQ=60)
                                                                                     ACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCA          >  SRR4302535.3437657/1‑94 (MQ=60)
                                                                                     ACCATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCA          >  SRR4302535.823128/1‑94 (MQ=60)
                                                                                       CATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAAC        >  SRR4302535.2469381/1‑94 (MQ=60)
                                                                                        ATGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACC       >  SRR4302535.3070898/1‑94 (MQ=60)
                                                                                         TGACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCG      >  SRR4302535.2592990/1‑94 (MQ=60)
                                                                                           ACACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTT    <  SRR4302535.2174548/94‑1 (MQ=60)
                                                                                             ACCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTC  >  SRR4302535.4256458/1‑94 (MQ=60)
                                                                                             ACCAACCGGTTCGGAGCGCGGACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTC  >  SRR4302535.1223157/1‑94 (MQ=60)
                                                                                             |                                                                                             
CGTCTCCGTCGGTGAAACTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTC  >  NC_000913/3806331‑3806517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑