Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,835,478 T→C V300A (GTA→GCA)  yicK → two‑module transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,835,4780TC96.6% 793.3 / 10.4 265V300A (GTA→GCA) yicKtwo‑module transport protein
Reads supporting (aligned to +/- strand):  ref base T (5/4);  new base C (128/128);  total (133/132)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.88e-01

Only 100 of 277 total aligned reads displayed.

TGTTAATCGCAGGCTATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGTACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTATCGGCATGCTTT  >  NC_000913/3835388‑3835567
                                                                                          |                                                                                         
tgTTAATCGCAGGCTATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCAc                                                                                         >  1:1070357/1‑93 (MQ=255)
tgTTAATCGCAGGCTATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCAc                                                                                         >  2:3352053/1‑93 (MQ=255)
tgTTAATCGCAGGCTATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCAc                                                                                         <  1:1738840/93‑1 (MQ=255)
 gTTAATCGCAGGCTATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACt                                                                                        >  1:2706685/1‑93 (MQ=255)
  ttAATCGCAGGCTATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTc                                                                                       >  1:620102/1‑93 (MQ=255)
   tAATCGCAGGCTATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCa                                                                                      <  1:2917172/93‑1 (MQ=255)
     aTCGCAGGCTATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATg                                                                                    <  2:4616402/93‑1 (MQ=255)
      tCGCAGGCTATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATgg                                                                                   <  1:1322995/93‑1 (MQ=255)
      tCGCAGGCTATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATgg                                                                                   >  2:3125559/1‑93 (MQ=255)
         cAGGCTATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGcga                                                                                <  1:550270/93‑1 (MQ=255)
         cAGGCTATTACATGAAACGCATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGcga                                                                                >  2:4560423/1‑93 (MQ=255)
          aGGCTATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGcgac                                                                               >  1:2544655/1‑93 (MQ=255)
          aGGCTATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGcgac                                                                               >  2:3804037/1‑93 (MQ=255)
           ggCTATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGTACTCATGGcgacg                                                                              >  1:500093/1‑93 (MQ=255)
           ggCTATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGcgacg                                                                              >  1:1655406/1‑93 (MQ=255)
              tATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCCTCGCGACGACt                                                                           >  2:1321181/1‑93 (MQ=255)
              tATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACt                                                                           >  1:869889/1‑93 (MQ=255)
               aTTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTc                                                                          >  1:21314/1‑93 (MQ=255)
               aTTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTc                                                                          <  2:1978685/93‑1 (MQ=255)
                ttACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTcc                                                                         <  2:1452872/93‑1 (MQ=255)
                ttACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTcc                                                                         <  2:4114657/93‑1 (MQ=255)
                 tACATGAAACGTATTGCCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCcg                                                                        <  1:3314927/93‑1 (MQ=255)
                  aCATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCcgg                                                                       <  2:387085/93‑1 (MQ=255)
                   cATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCcggc                                                                      >  2:2006169/1‑93 (MQ=255)
                   cATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCcggc                                                                      >  1:81957/1‑93 (MQ=255)
                   cATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCcggc                                                                      <  2:2517224/93‑1 (MQ=255)
                    aTGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCcggcg                                                                     >  1:3928435/1‑93 (MQ=255)
                     tGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCcggcgg                                                                    <  1:1966401/93‑1 (MQ=255)
                      gAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGt                                                                   >  1:3147491/1‑93 (MQ=255)
                       aaaCGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGtt                                                                  <  2:1156402/93‑1 (MQ=255)
                       aaaCGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGtt                                                                  >  1:2459990/1‑93 (MQ=255)
                         aCGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGa                                                                <  2:2425491/93‑1 (MQ=255)
                         aCGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGa                                                                <  1:3389332/93‑1 (MQ=255)
                         aCGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGa                                                                <  1:2994453/93‑1 (MQ=255)
                            tATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCt                                                             >  1:4147721/1‑93 (MQ=255)
                            tATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCt                                                             <  2:835397/93‑1 (MQ=255)
                             aTTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTg                                                            <  1:2334940/93‑1 (MQ=255)
                                ggCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGaa                                                         <  2:2592451/93‑1 (MQ=255)
                                  cAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAAtt                                                       <  1:3094116/93‑1 (MQ=255)
                                  cAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAAtt                                                       <  2:3583449/93‑1 (MQ=255)
                                  cAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAAtt                                                       <  2:2834733/93‑1 (MQ=255)
                                   aaGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTg                                                      >  2:2979077/1‑93 (MQ=255)
                                    aGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGc                                                     <  2:4471763/93‑1 (MQ=255)
                                    aGCGACTATTAATGCTCATTGCTATCGTGAGAGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGc                                                     <  1:2764346/93‑1 (MQ=255)
                                       gACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCaaa                                                  <  1:4573069/93‑1 (MQ=255)
                                         cTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAAtt                                                >  1:2098312/1‑93 (MQ=255)
                                         cTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAAtt                                                >  2:1516665/1‑93 (MQ=255)
                                          tATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTc                                               <  1:2817302/93‑1 (MQ=255)
                                           aTTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCt                                              <  1:4198912/93‑1 (MQ=255)
                                           aTTAAGTCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGTCTCCGGCGGTTGAGCTGGAATTGCAAATTCt                                              <  1:1593770/93‑1 (MQ=255)
                                            ttAATGCTCTTTGCTATCGTGAATGGAATGTGTTTTTAAGCCAGCACACTCATGGCGACGAATCCGGCGGTTGAGCTAGAATAGCATATTCtt                                             >  2:3737281/1‑93 (MQ=255)
                                            ttAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCtt                                             >  2:4322304/1‑93 (MQ=255)
                                            ttAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCtt                                             >  2:1830522/1‑93 (MQ=255)
                                             tAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTa                                            <  2:3206121/93‑1 (MQ=255)
                                              aaTGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTaa                                           <  1:1533296/93‑1 (MQ=255)
                                                tGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGTATTGCAACTTCTTAATg                                         >  2:2215013/1‑93 (MQ=255)
                                                   tCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGTACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCa                                      >  2:2200021/1‑93 (MQ=255)
                                                   tCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCa                                      <  1:1371536/93‑1 (MQ=255)
                                                   tCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCa                                      <  1:2185597/93‑1 (MQ=255)
                                                    cATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCAt                                     <  1:1083119/93‑1 (MQ=255)
                                                    cATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCAt                                     >  1:1233762/1‑93 (MQ=255)
                                                    cATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCAt                                     <  1:3742233/93‑1 (MQ=255)
                                                     aTTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATc                                    <  2:3384631/93‑1 (MQ=255)
                                                        gCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTc                                 <  2:3276952/93‑1 (MQ=255)
                                                         cTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTcc                                <  2:75980/93‑1 (MQ=255)
                                                         cTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTcc                                <  1:3853827/93‑1 (MQ=255)
                                                           aTCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCtt                              <  2:172949/93‑1 (MQ=255)
                                                            tCGTGAGTGGAATGTGTTTTTACGCCAGCGCTCTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTg                             <  1:4072081/93‑1 (MQ=255)
                                                             cGTGAGTGGAATGTGTTTTTACGCCAGCGCTCTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTgg                            <  2:1673914/93‑1 (MQ=255)
                                                             cGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTgg                            <  2:236732/93‑1 (MQ=255)
                                                             cGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTgg                            <  1:659026/93‑1 (MQ=255)
                                                              gTGAGTGGAATGTGTTTTTACGCCAGCGTACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGt                           <  1:1393590/93‑1 (MQ=255)
                                                              gTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGt                           >  1:3201167/1‑93 (MQ=255)
                                                              gTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGt                           <  1:3636622/93‑1 (MQ=255)
                                                               tGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTa                          <  2:2716857/93‑1 (MQ=255)
                                                                gAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTAt                         <  2:4067647/93‑1 (MQ=255)
                                                                gAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTAt                         <  1:893179/93‑1 (MQ=255)
                                                                   tGGAATGTGTTTTTACGCCAGCGCACTCCTGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATtct                      >  2:1281526/1‑93 (MQ=255)
                                                                    ggAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATtctc                     <  1:4393908/93‑1 (MQ=255)
                                                                      aaTGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCtg                   >  2:2274648/1‑93 (MQ=255)
                                                                       aTGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCtgt                  <  1:3616482/93‑1 (MQ=255)
                                                                       aTGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCtgt                  <  1:987515/93‑1 (MQ=255)
                                                                        tgtgTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCtgtg                 >  1:684127/1‑93 (MQ=255)
                                                                        tgtgTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCtgtg                 >  1:485566/1‑93 (MQ=255)
                                                                         gtgtTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTgg                >  2:4459943/1‑93 (MQ=255)
                                                                           gtTTTTACGCCAGCGTACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTa              >  2:1975868/1‑93 (MQ=255)
                                                                           gtTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTa              >  1:1606726/1‑93 (MQ=255)
                                                                           gtTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTa              <  1:3318520/93‑1 (MQ=255)
                                                                            tttttACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTAt             <  2:4388670/93‑1 (MQ=255)
                                                                              tttACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTATCg           >  1:2223577/1‑93 (MQ=255)
                                                                                 tcGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTATCGGCa        <  1:3499457/92‑1 (MQ=255)
                                                                                 aCGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTATCGGCa        <  2:422198/93‑1 (MQ=255)
                                                                                 aCGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTATCGGCa        <  1:1501664/93‑1 (MQ=255)
                                                                                 aCGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTATCGGCa        >  1:3593427/1‑93 (MQ=255)
                                                                                 aCGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTATCGGCa        <  2:1266281/93‑1 (MQ=255)
                                                                                    acAGCGCACTCATGGCGACGACTCAGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTATCGGCATGc     <  2:489775/92‑1 (MQ=255)
                                                                                     cAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTATCGGCATGCt    >  2:1762001/1‑93 (MQ=255)
                                                                                      aGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTATCGGCATGCtt   <  2:4294640/93‑1 (MQ=255)
                                                                                       gcgcACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTATCGGCATGCttt  >  1:2000138/1‑93 (MQ=255)
                                                                                       gcgcACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTATCGGCATGCttt  >  1:1865708/1‑93 (MQ=255)
                                                                                          |                                                                                         
TGTTAATCGCAGGCTATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGTACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTATCGGCATGCTTT  >  NC_000913/3835388‑3835567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGATGTTAATCGCAGGCTATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGTACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTATCGGCATGCTTTATT  >  NC_000913/3835385‑3835570
                                                                                             |                                                                                            
TGATGTTAATCGCAGGCTATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGC                                                                                              >  SRR4302535.270999/1‑94 (MQ=60)
 GATGTTAATCGCAGGCTATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCA                                                                                             <  SRR4302535.3033451/94‑1 (MQ=60)
 GATGTTAATCGCAGGCTATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCA                                                                                             <  SRR4302535.406799/94‑1 (MQ=60)
  ATGTTAATCGCAGGCTATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCAC                                                                                            >  SRR4302535.1077967/1‑94 (MQ=60)
   TGTTAATCGCAGGCTATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACT                                                                                           <  SRR4302535.1751406/94‑1 (MQ=60)
   TGTTAATCGCAGGCTATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACT                                                                                           >  SRR4302535.2726025/1‑94 (MQ=60)
    GTTAATCGCAGGCTATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTC                                                                                          >  SRR4302535.624414/1‑94 (MQ=60)
     GTAATCGCAAGCTATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCA                                                                                         <  SRR4302535.3148950/94‑1 (MQ=60)
      TAATCGCAGGCTATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGTGCACTCAT                                                                                        >  SRR4302535.186893/1‑94 (MQ=60)
      TAATCGCAGGCTATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCAT                                                                                        >  SRR4302535.3349029/1‑94 (MQ=60)
      TAATCGCAGGCTATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCAT                                                                                        <  SRR4302535.2937764/94‑1 (MQ=60)
       AATCGCAGGCTATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATG                                                                                       >  SRR4302535.2939216/1‑94 (MQ=60)
       AATCGCAGGCTATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATG                                                                                       <  SRR4302535.1139225/94‑1 (MQ=60)
       AATCGCAGGCTATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATG                                                                                       >  SRR4302535.3632111/1‑94 (MQ=60)
        ATCGCAGGCTATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGG                                                                                      <  SRR4302535.2167465/94‑1 (MQ=60)
        ATCGCAGGCTATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGG                                                                                      >  SRR4302535.2447879/1‑94 (MQ=60)
         TCGCAGGCTATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGC                                                                                     <  SRR4302535.1332437/94‑1 (MQ=60)
            CAGGCTATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGAC                                                                                  <  SRR4302535.554120/94‑1 (MQ=60)
            CAGGCTATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGAC                                                                                  <  SRR4302535.4604952/94‑1 (MQ=60)
            CAGGCTATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGAC                                                                                  >  SRR4302535.2562975/1‑94 (MQ=60)
             AGGCTATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGTACTCATGGCGACG                                                                                 >  SRR4302535.503597/1‑94 (MQ=60)
             AGGCTATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACG                                                                                 >  SRR4302535.1580667/1‑94 (MQ=60)
             AGGCTATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACG                                                                                 >  SRR4302535.1667379/1‑94 (MQ=60)
              GGCTATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGA                                                                                >  SRR4302535.3427910/1‑94 (MQ=60)
               GCTATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGAC                                                                               <  SRR4302535.3339602/94‑1 (MQ=60)
                CTATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACT                                                                              >  SRR4302535.876052/1‑94 (MQ=60)
                CTATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACT                                                                              >  SRR4302535.3573689/1‑94 (MQ=60)
                 TATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTC                                                                             >  SRR4302535.2278374/1‑94 (MQ=60)
                 TATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTC                                                                             >  SRR4302535.668494/1‑94 (MQ=60)
                 TATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTC                                                                             >  SRR4302535.21471/1‑94 (MQ=60)
                  ATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGTACTCATGGCGACGACTCC                                                                            <  SRR4302535.894827/94‑1 (MQ=60)
                  ATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCC                                                                            <  SRR4302535.226537/94‑1 (MQ=60)
                   TTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCG                                                                           <  SRR4302535.499343/94‑1 (MQ=60)
                   TTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCG                                                                           <  SRR4302535.964244/94‑1 (MQ=60)
                    TACATGAAACGTATTGCCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGG                                                                          <  SRR4302535.3337990/94‑1 (MQ=60)
                     ACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGCATGTGTTTTTACGCCAGCGTACTCATGGCGACGACTCCGGC                                                                         >  SRR4302535.1361907/1‑94 (MQ=60)
                     ACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGC                                                                         >  SRR4302535.717243/1‑94 (MQ=60)
                     ACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGC                                                                         >  SRR4302535.2144534/1‑94 (MQ=60)
                     ACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGC                                                                         >  SRR4302535.82541/1‑94 (MQ=60)
                      CATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCG                                                                        <  SRR4302535.3903533/94‑1 (MQ=60)
                      CATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCG                                                                        >  SRR4302535.1549311/1‑94 (MQ=60)
                      CATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCG                                                                        >  SRR4302535.3955268/1‑94 (MQ=60)
                       ATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGG                                                                       >  SRR4302535.2869386/1‑94 (MQ=60)
                        TGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGT                                                                      <  SRR4302535.1980575/94‑1 (MQ=60)
                        TGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGT                                                                      >  SRR4302535.3169373/1‑94 (MQ=60)
                         GAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTT                                                                     >  SRR4302535.2477726/1‑94 (MQ=60)
                         GAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTT                                                                     >  SRR4302535.2446903/1‑94 (MQ=60)
                         GAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTT                                                                     >  SRR4302535.3712103/1‑94 (MQ=60)
                         GAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTT                                                                     >  SRR4302535.3849431/1‑94 (MQ=60)
                         GAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTT                                                                     <  SRR4302535.3400047/94‑1 (MQ=60)
                           AACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGA                                                                   >  SRR4302535.2225834/1‑94 (MQ=60)
                            ACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAG                                                                  <  SRR4302535.3412910/94‑1 (MQ=60)
                            ACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAG                                                                  <  SRR4302535.2127244/94‑1 (MQ=60)
                            ACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAG                                                                  <  SRR4302535.3015446/94‑1 (MQ=60)
                              GTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCT                                                                >  SRR4302535.3769350/1‑94 (MQ=60)
                              GTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCT                                                                >  SRR4302535.4176121/1‑94 (MQ=60)
                               TATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTG                                                               >  SRR4302535.1936589/1‑94 (MQ=60)
                                ATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGG                                                              <  SRR4302535.2351651/94‑1 (MQ=60)
                                     CAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTG                                                         <  SRR4302535.3115663/94‑1 (MQ=60)
                                     CAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTG                                                         >  SRR4302535.3304436/1‑94 (MQ=60)
                                     CAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTG                                                         >  SRR4302535.2753262/1‑94 (MQ=60)
                                      AAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGC                                                        <  SRR4302535.526824/94‑1 (MQ=60)
                                      AAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGC                                                        >  SRR4302535.4394055/1‑94 (MQ=60)
                                       AGCGACTATTAATGCTCATTGCTATCGTGAGAGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCA                                                       <  SRR4302535.2784038/94‑1 (MQ=60)
                                        GCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTGACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAA                                                      >  SRR4302535.2529646/1‑94 (MQ=60)
                                          GACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAAT                                                    <  SRR4302535.4604243/94‑1 (MQ=60)
                                          GACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAAT                                                    >  SRR4302535.495014/1‑94 (MQ=60)
                                           ACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATT                                                   >  SRR4302535.3650794/1‑94 (MQ=60)
                                           ACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATT                                                   >  SRR4302535.2113446/1‑94 (MQ=60)
                                           ACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATT                                                   >  SRR4302535.162773/1‑94 (MQ=60)
                                            CTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTC                                                  <  SRR4302535.2895955/94‑1 (MQ=60)
                                             TATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCT                                                 >  SRR4302535.2736321/1‑94 (MQ=60)
                                             TATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCT                                                 <  SRR4302535.620251/94‑1 (MQ=60)
                                             TATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCT                                                 <  SRR4302535.2837311/94‑1 (MQ=60)
                                             TATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCT                                                 <  SRR4302535.2057771/94‑1 (MQ=60)
                                              ATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTT                                                <  SRR4302535.4227647/94‑1 (MQ=60)
                                              ATTAAGTCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGTCTCCGGCGGTTGAGCTGGAATTGCAAATTCTT                                                <  SRR4302535.1605271/94‑1 (MQ=60)
                                                TAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAA                                              >  SRR4302535.1447919/1‑94 (MQ=60)
                                                 AATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAAT                                             <  SRR4302535.1544344/94‑1 (MQ=60)
                                                 AATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAAT                                             <  SRR4302535.1160864/94‑1 (MQ=60)
                                                  ATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATG                                            <  SRR4302535.1727236/94‑1 (MQ=60)
                                                   TGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGC                                           >  SRR4302535.4190592/1‑94 (MQ=60)
                                                    GCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGTACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCC                                          <  SRR4302535.248144/94‑1 (MQ=60)
                                                    GCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCC                                          <  SRR4302535.552552/94‑1 (MQ=60)
                                                      TCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCAT                                        <  SRR4302535.1381333/94‑1 (MQ=60)
                                                      TCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCAT                                        >  SRR4302535.3193287/1‑94 (MQ=60)
                                                      TCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCAT                                        >  SRR4302535.1242581/1‑94 (MQ=60)
                                                      TCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCAT                                        <  SRR4302535.2201174/94‑1 (MQ=60)
                                                       CATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATC                                       >  SRR4302535.662002/1‑94 (MQ=60)
                                                       CATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATC                                       <  SRR4302535.3768126/94‑1 (MQ=60)
                                                       CATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATC                                       <  SRR4302535.1090818/94‑1 (MQ=60)
                                                            CTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCT                                  >  SRR4302535.670294/1‑94 (MQ=60)
                                                            CTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCT                                  <  SRR4302535.3880367/94‑1 (MQ=60)
                                                            CTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCT                                  >  SRR4302535.1620354/1‑94 (MQ=60)
                                                            CTATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCT                                  >  SRR4302535.83263/1‑94 (MQ=60)
                                                             TATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTT                                 >  SRR4302535.2098106/1‑94 (MQ=60)
                                                             TATCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTT                                 >  SRR4302535.1519210/1‑94 (MQ=60)
                                                               TCGTGAGTGGAATGTGTTTTTACGCCAGCGCTCTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGG                               <  SRR4302535.4099947/94‑1 (MQ=60)
                                                               TCGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGG                               <  SRR4302535.1461675/94‑1 (MQ=60)
                                                                CGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGT                              <  SRR4302535.663624/94‑1 (MQ=60)
                                                                CGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGT                              >  SRR4302535.1794445/1‑94 (MQ=60)
                                                                CGTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGT                              >  SRR4302535.3223331/1‑94 (MQ=60)
                                                                 GTGAGTGGAATGTGTTTTTACGCCAGCGTACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTA                             <  SRR4302535.1403540/94‑1 (MQ=60)
                                                                 GTGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTA                             <  SRR4302535.3661822/94‑1 (MQ=60)
                                                                  TGAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTAT                            <  SRR4302535.280835/94‑1 (MQ=60)
                                                                   GAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATT                           <  SRR4302535.899508/94‑1 (MQ=60)
                                                                   GAGTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATT                           <  SRR4302535.140007/94‑1 (MQ=60)
                                                                     GTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCT                         >  SRR4302535.2946206/1‑94 (MQ=60)
                                                                     GTGGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCT                         >  SRR4302535.1606647/1‑94 (MQ=60)
                                                                       GGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCCTCTTCCTTGGTATTCTCT                       >  SRR4302535.4117/1‑94 (MQ=60)
                                                                       GGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCT                       <  SRR4302535.4423974/94‑1 (MQ=60)
                                                                       GGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCT                       >  SRR4302535.3444876/1‑94 (MQ=60)
                                                                       GGAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCT                       <  SRR4302535.1316908/94‑1 (MQ=60)
                                                                        GAATGTGTTTTTACGCCAGCGCCCTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTG                      <  SRR4302535.4184184/94‑1 (MQ=60)
                                                                        GAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTG                      >  SRR4302535.842481/1‑94 (MQ=60)
                                                                        GAATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTG                      >  SRR4302535.754711/1‑94 (MQ=60)
                                                                         AATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGT                     >  SRR4302535.3530808/1‑94 (MQ=60)
                                                                          ATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTG                    >  SRR4302535.688915/1‑94 (MQ=60)
                                                                          ATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTG                    >  SRR4302535.488969/1‑94 (MQ=60)
                                                                          ATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTG                    <  SRR4302535.994531/94‑1 (MQ=60)
                                                                          ATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTG                    >  SRR4302535.3654040/1‑94 (MQ=60)
                                                                          ATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTG                    <  SRR4302535.3641554/94‑1 (MQ=60)
                                                                          ATGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTG                    <  SRR4302535.3067149/94‑1 (MQ=60)
                                                                           TGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATGTTCCTTGGTATTCTCTGTGG                   >  SRR4302535.3248548/1‑94 (MQ=60)
                                                                           TGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGG                   >  SRR4302535.1080059/1‑94 (MQ=60)
                                                                           TGTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGG                   >  SRR4302535.4657578/1‑94 (MQ=60)
                                                                            GTGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGT                  >  SRR4302535.3688092/1‑94 (MQ=60)
                                                                             TGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTA                 >  SRR4302535.1618319/1‑94 (MQ=60)
                                                                             TGTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTA                 >  SRR4302535.1926203/1‑94 (MQ=60)
                                                                              GTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTAT                >  SRR4302535.1995467/1‑94 (MQ=60)
                                                                              GTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTAT                <  SRR4302535.4139572/94‑1 (MQ=60)
                                                                              GTTTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTAT                <  SRR4302535.3341609/94‑1 (MQ=60)
                                                                                TTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTATCG              <  SRR4302535.62580/94‑1 (MQ=60)
                                                                                TTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTATCG              >  SRR4302535.2239404/1‑94 (MQ=60)
                                                                                TTTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTATCG              <  SRR4302535.320650/94‑1 (MQ=60)
                                                                                 TTTACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTATCGG             >  SRR4302535.1356814/1‑94 (MQ=60)
                                                                                   TACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTATCGGCA           >  SRR4302535.3618367/1‑94 (MQ=60)
                                                                                   TACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTATCGGCA           >  SRR4302535.3942033/1‑94 (MQ=60)
                                                                                    TCGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTATCGGCAT          <  SRR4302535.3523787/94‑1 (MQ=60)
                                                                                    ATGCCAGCGCACTCAGTGCGACGGCTTCGGCGGTTGAGCTTGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTATCGGCAT          <  SRR4302535.1512467/94‑1 (MQ=60)
                                                                                    ACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTATCGGCAT          <  SRR4302535.1512466/94‑1 (MQ=60)
                                                                                    ACGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTATCGGCAT          <  SRR4302535.4290823/94‑1 (MQ=60)
                                                                                     CGCCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTATCGGCATG         <  SRR4302535.4067047/94‑1 (MQ=60)
                                                                                       CCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTATCGGCATGCT       <  SRR4302535.1704330/94‑1 (MQ=60)
                                                                                       CCAGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTATCGGCATGCT       <  SRR4302535.905133/94‑1 (MQ=60)
                                                                                        CAGCGCACTCATGTCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTATCGGCATGCTT      <  SRR4302535.2635065/94‑1 (MQ=60)
                                                                                         AGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTATCGGCATGCTTT     <  SRR4302535.4503534/94‑1 (MQ=60)
                                                                                         AGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTATCGGCATGCTTT     >  SRR4302535.2014551/1‑94 (MQ=60)
                                                                                         AGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTATCGGCATGCTTT     >  SRR4302535.1879155/1‑94 (MQ=60)
                                                                                         AGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTATCGGCATGCTTT     >  SRR4302535.2167794/1‑94 (MQ=60)
                                                                                         AGCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTATCGGCATGCTTT     <  SRR4302535.1262109/94‑1 (MQ=60)
                                                                                          GCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTATCGGCATGCTTTA    <  SRR4302535.280585/94‑1 (MQ=60)
                                                                                          GCGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTATCGGCATGCTTTA    <  SRR4302535.312441/94‑1 (MQ=60)
                                                                                           CGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTATCGGCATGCTTTAT   <  SRR4302535.2003362/94‑1 (MQ=60)
                                                                                           CGCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTATCGGCATGCTTTAT   <  SRR4302535.750209/94‑1 (MQ=60)
                                                                                            GCACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTATCGGCATGCTTTATT  >  SRR4302535.2656050/1‑94 (MQ=60)
                                                                                             |                                                                                            
TGATGTTAATCGCAGGCTATTACATGAAACGTATTGGCAAGCGACTATTAATGCTCATTGCTATCGTGAGTGGAATGTGTTTTTACGCCAGCGTACTCATGGCGACGACTCCGGCGGTTGAGCTGGAATTGCAAATTCTTAATGCCATCTTCCTTGGTATTCTCTGTGGTATCGGCATGCTTTATT  >  NC_000913/3835385‑3835570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑