Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,849,668 G→A G248G (GGC→GGT)  ilvB ← acetolactate synthase I large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,849,6680GA100.0% 801.8 / NA 227G248G (GGC→GGT) ilvBacetolactate synthase I large subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (119/108);  total (119/108)

Only 100 of 234 total aligned reads displayed.

CAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCC  >  NC_000913/3849578‑3849757
                                                                                          |                                                                                         
caaATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATAcc                                                                                         <  1:1841447/93‑1 (MQ=255)
  aaTCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCa                                                                                       <  1:1055159/93‑1 (MQ=255)
  aaTCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCa                                                                                       >  1:3427493/1‑93 (MQ=255)
  aaTCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCa                                                                                       <  2:3545781/93‑1 (MQ=255)
   aTCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAg                                                                                      >  2:1664685/1‑93 (MQ=255)
        cctcctGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCAAGCGCCa                                                                                 <  2:1770334/93‑1 (MQ=255)
         ctcctGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCAt                                                                                >  2:3073910/1‑93 (MQ=255)
         ctcctGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCAt                                                                                <  2:1466501/93‑1 (MQ=255)
         ctcctGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCAt                                                                                >  2:2188891/1‑93 (MQ=255)
         ctcctGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCAt                                                                                >  1:2216717/1‑93 (MQ=255)
          tcctGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCAtt                                                                               >  2:3637048/1‑93 (MQ=255)
          tcctGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCAtt                                                                               <  1:2754396/93‑1 (MQ=255)
           cctGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTa                                                                              <  1:895942/93‑1 (MQ=255)
             tGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTaaa                                                                            <  2:3715153/93‑1 (MQ=255)
             tGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTaaa                                                                            >  1:683182/1‑93 (MQ=255)
             tGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTaaa                                                                            <  1:3641983/93‑1 (MQ=255)
              gCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAg                                                                           <  1:4250769/93‑1 (MQ=255)
              gCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAg                                                                           <  2:273662/93‑1 (MQ=255)
              gCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAg                                                                           <  2:2915402/93‑1 (MQ=255)
               cAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGt                                                                          <  1:2347351/93‑1 (MQ=255)
                aaaaTATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTc                                                                         <  2:864906/93‑1 (MQ=255)
                 aaaTATAGTTGGTGCTGTGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCa                                                                        <  1:4507848/93‑1 (MQ=255)
                  aaTATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAt                                                                       <  1:72183/93‑1 (MQ=255)
                  aaTATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAt                                                                       >  2:1251652/1‑93 (MQ=255)
                  aaTATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAt                                                                       >  1:2962122/1‑93 (MQ=255)
                  aaTATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAt                                                                       >  2:3876977/1‑93 (MQ=255)
                  aaTATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAt                                                                       <  2:3782939/93‑1 (MQ=255)
                  aaTATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAt                                                                       <  2:4420207/93‑1 (MQ=255)
                   atatAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAtg                                                                      >  2:3457190/1‑93 (MQ=255)
                   atatAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAtg                                                                      <  2:1847832/93‑1 (MQ=255)
                   atatAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAtg                                                                      >  2:1531955/1‑93 (MQ=255)
                    tataGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAtgg                                                                     >  2:3504025/1‑93 (MQ=255)
                    tataGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAtgg                                                                     >  1:3064183/1‑93 (MQ=255)
                    tataGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAtgg                                                                     >  1:412689/1‑93 (MQ=255)
                     ataGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAtggt                                                                    <  1:1270443/93‑1 (MQ=255)
                     ataGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAtggt                                                                    <  1:2884836/93‑1 (MQ=255)
                      taGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAtggtg                                                                   >  2:2074787/1‑93 (MQ=255)
                         ttGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTa                                                                <  2:70594/93‑1 (MQ=255)
                         ttGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTa                                                                <  1:1933532/93‑1 (MQ=255)
                         ttGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTa                                                                <  1:1537870/93‑1 (MQ=255)
                          tGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAg                                                               >  1:4312111/1‑93 (MQ=255)
                            gTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGc                                                             <  2:3295646/93‑1 (MQ=255)
                             tgctgcGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCa                                                            >  1:3718073/1‑93 (MQ=255)
                              gctgcGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAg                                                           <  1:4406976/93‑1 (MQ=255)
                              gctgcGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAg                                                           >  1:4092466/1‑93 (MQ=255)
                              gctgcGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAg                                                           <  1:391676/93‑1 (MQ=255)
                              gctgcGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGAAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAg                                                           >  1:4523837/1‑93 (MQ=255)
                                tgcGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGtt                                                         <  1:476665/93‑1 (MQ=255)
                                tgcGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGtt                                                         <  2:3010796/93‑1 (MQ=255)
                                 gcgcACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTg                                                        <  1:2331718/93‑1 (MQ=255)
                                 gcccACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTg                                                        <  2:1628704/90‑1 (MQ=255)
                                   gcACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTgcg                                                      >  1:3798757/1‑93 (MQ=255)
                                    cACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTgcgc                                                     >  2:1166244/1‑93 (MQ=255)
                                     aCGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCt                                                    >  2:4129801/1‑93 (MQ=255)
                                     aCGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCt                                                    <  1:926411/93‑1 (MQ=255)
                                      cGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCtt                                                   <  1:2497355/93‑1 (MQ=255)
                                       gCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCttt                                                  <  1:83724/93‑1 (MQ=255)
                                       gCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCttt                                                  <  1:345426/93‑1 (MQ=255)
                                       gCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCttt                                                  <  2:2699314/93‑1 (MQ=255)
                                         cGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTtct                                                >  1:1955859/1‑93 (MQ=255)
                                         cGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTtct                                                >  2:1057972/1‑93 (MQ=255)
                                          gTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTtctc                                               >  1:3273354/1‑93 (MQ=255)
                                            gCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCg                                             <  2:262100/93‑1 (MQ=255)
                                               tCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACACAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCa                                          >  2:2020241/1‑93 (MQ=255)
                                                   cAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTc                                      <  2:2424472/93‑1 (MQ=255)
                                                    aGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCa                                     >  2:4636504/1‑93 (MQ=255)
                                                     gCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCAc                                    <  2:4340895/93‑1 (MQ=255)
                                                     gCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCAc                                    >  1:1148773/1‑93 (MQ=255)
                                                        tACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCa                                 <  1:955206/93‑1 (MQ=255)
                                                        tACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCa                                 <  1:1545278/93‑1 (MQ=255)
                                                        tACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCa                                 >  1:4194470/1‑93 (MQ=255)
                                                        tACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCa                                 <  2:2061026/93‑1 (MQ=255)
                                                        tACCCAGCGACAACGGATGCGCTTTTGGAAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCa                                 >  2:4350525/1‑93 (MQ=255)
                                                            cAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCg                             >  2:389829/1‑93 (MQ=255)
                                                              gCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTg                           >  1:744236/1‑93 (MQ=255)
                                                                  cAACGGATGCGCTTTTGGTAACATACCCAGCGCCATTAAAGTCCTGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCggg                       >  1:1714048/1‑93 (MQ=255)
                                                                   aaCGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCgggg                      >  1:2139511/1‑92 (MQ=255)
                                                                   aaCGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGgc                      >  2:637689/1‑93 (MQ=255)
                                                                     cGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGgcgc                    >  1:57168/1‑93 (MQ=255)
                                                                       gATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCAt                  <  1:1003246/93‑1 (MQ=255)
                                                                        aTGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCAtt                 <  2:3357472/93‑1 (MQ=255)
                                                                         tGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTg                >  2:648283/1‑93 (MQ=255)
                                                                          gcgcTTTTGGAAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGa               >  1:518532/1‑93 (MQ=255)
                                                                           cgcTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGAt              <  1:1678588/93‑1 (MQ=255)
                                                                           cgcTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGAt              <  1:361719/93‑1 (MQ=255)
                                                                             cTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATca            >  2:3701244/1‑93 (MQ=255)
                                                                              ttttGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATcac           >  2:437875/1‑93 (MQ=255)
                                                                              ttttGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATcac           <  2:4012950/93‑1 (MQ=255)
                                                                                ttGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATGGATCacac         <  2:4577639/93‑1 (MQ=255)
                                                                                  ggCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACAccg       >  1:3190671/1‑93 (MQ=255)
                                                                                  ggCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACAccg       >  2:3122074/1‑93 (MQ=255)
                                                                                  ggCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACAccg       >  2:2325979/1‑93 (MQ=255)
                                                                                   gCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACAccgc      >  1:3851640/1‑93 (MQ=255)
                                                                                     aaCATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACAccgccg    >  2:895539/1‑93 (MQ=255)
                                                                                      aCATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACcgccgc   >  1:2246958/1‑93 (MQ=255)
                                                                                      aCATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACcgccgc   <  1:3106670/93‑1 (MQ=255)
                                                                                      aCATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACcgccgc   <  1:3941498/93‑1 (MQ=255)
                                                                                       cATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCgccgcc  >  2:4020790/1‑93 (MQ=255)
                                                                                       cATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCgccgcc  >  1:1752869/1‑93 (MQ=255)
                                                                                       cATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCgccgcc  <  2:1799634/93‑1 (MQ=255)
                                                                                          |                                                                                         
CAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCC  >  NC_000913/3849578‑3849757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCCCAG  >  NC_000913/3849575‑3849760
                                                                                             |                                                                                            
CAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATA                                                                                              <  SRR4302535.2749529/94‑1 (MQ=60)
 AACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATAC                                                                                             >  SRR4302535.316116/1‑94 (MQ=60)
  ACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACC                                                                                            >  SRR4302535.2851057/1‑94 (MQ=60)
   CAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCC                                                                                           <  SRR4302535.670901/94‑1 (MQ=60)
   CAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCC                                                                                           <  SRR4302535.1706997/94‑1 (MQ=60)
   CAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCC                                                                                           <  SRR4302535.1854728/94‑1 (MQ=60)
    AAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCA                                                                                          >  SRR4302535.3451319/1‑94 (MQ=60)
    AAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCA                                                                                          <  SRR4302535.3243735/94‑1 (MQ=60)
     AATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAG                                                                                         <  SRR4302535.1062662/94‑1 (MQ=60)
      ATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGC                                                                                        >  SRR4302535.828274/1‑94 (MQ=60)
      ATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGC                                                                                        >  SRR4302535.1616524/1‑94 (MQ=60)
      ATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGC                                                                                        >  SRR4302535.205038/1‑94 (MQ=60)
        CCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGC                                                                                      >  SRR4302535.1891381/1‑94 (MQ=60)
         CGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCC                                                                                     >  SRR4302535.733188/1‑94 (MQ=60)
          GCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCA                                                                                    >  SRR4302535.1555285/1‑94 (MQ=60)
           CCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCAT                                                                                   >  SRR4302535.3852946/1‑94 (MQ=60)
           CCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCAT                                                                                   <  SRR4302535.1848472/94‑1 (MQ=60)
           CCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCAT                                                                                   >  SRR4302535.2232498/1‑94 (MQ=60)
             TCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTA                                                                                 <  SRR4302535.2774029/94‑1 (MQ=60)
              CCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAA                                                                                <  SRR4302535.902295/94‑1 (MQ=60)
               CTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAA                                                                               >  SRR4302535.687965/1‑94 (MQ=60)
                TGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAG                                                                              <  SRR4302535.3667207/94‑1 (MQ=60)
                TGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAG                                                                              >  SRR4302535.1201603/1‑94 (MQ=60)
                 GCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGT                                                                             <  SRR4302535.4279886/94‑1 (MQ=60)
                  CAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTC                                                                            <  SRR4302535.2364177/94‑1 (MQ=60)
                  CAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTC                                                                            >  SRR4302535.2235067/1‑94 (MQ=60)
                    AAATATAGTTGGTGCTGTGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAT                                                                          <  SRR4302535.4538783/94‑1 (MQ=60)
                    AAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAT                                                                          >  SRR4302535.3635129/1‑94 (MQ=60)
                    AAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAT                                                                          <  SRR4302535.1128338/94‑1 (MQ=60)
                    AAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAT                                                                          >  SRR4302535.2982914/1‑94 (MQ=60)
                     AATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATG                                                                         <  SRR4302535.72709/94‑1 (MQ=60)
                      ATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGG                                                                        >  SRR4302535.415588/1‑94 (MQ=60)
                      ATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGG                                                                        >  SRR4302535.3085601/1‑94 (MQ=60)
                      ATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGG                                                                        <  SRR4302535.543939/94‑1 (MQ=60)
                      ATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGG                                                                        >  SRR4302535.513655/1‑94 (MQ=60)
                      ATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGG                                                                        >  SRR4302535.1666091/1‑94 (MQ=60)
                      ATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGG                                                                        <  SRR4302535.1763120/94‑1 (MQ=60)
                       TATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGT                                                                       <  SRR4302535.4549720/94‑1 (MQ=60)
                        ATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTG                                                                      <  SRR4302535.4380390/94‑1 (MQ=60)
                        ATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTG                                                                      <  SRR4302535.4044239/94‑1 (MQ=60)
                        ATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTG                                                                      <  SRR4302535.2905246/94‑1 (MQ=60)
                        ATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTG                                                                      <  SRR4302535.4165023/94‑1 (MQ=60)
                        ATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTG                                                                      <  SRR4302535.3081208/94‑1 (MQ=60)
                        ATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTG                                                                      <  SRR4302535.3956315/94‑1 (MQ=60)
                        ATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTG                                                                      <  SRR4302535.1279520/94‑1 (MQ=60)
                         TAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGG                                                                     <  SRR4302535.3534122/94‑1 (MQ=60)
                          AGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGT                                                                    <  SRR4302535.2605848/94‑1 (MQ=60)
                            TTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAG                                                                  <  SRR4302535.1548951/94‑1 (MQ=60)
                            TTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAG                                                                  <  SRR4302535.1947457/94‑1 (MQ=60)
                            TTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAG                                                                  >  SRR4302535.4341611/1‑94 (MQ=60)
                             TGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGG                                                                 <  SRR4302535.1527159/94‑1 (MQ=60)
                               GTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCA                                                               >  SRR4302535.3743834/1‑94 (MQ=60)
                                TGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAG                                                              >  SRR4302535.3451463/1‑94 (MQ=60)
                                TGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAG                                                              >  SRR4302535.4120471/1‑94 (MQ=60)
                                TGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGAAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAG                                                              >  SRR4302535.4554831/1‑94 (MQ=60)
                                 GCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGT                                                             <  SRR4302535.928072/94‑1 (MQ=60)
                                 GCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGT                                                             <  SRR4302535.4437135/94‑1 (MQ=60)
                                 GCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGT                                                             <  SRR4302535.394404/94‑1 (MQ=60)
                                   TGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTG                                                           <  SRR4302535.480009/94‑1 (MQ=60)
                                    GCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGC                                                          <  SRR4302535.2348396/94‑1 (MQ=60)
                                     CGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCG                                                         >  SRR4302535.3824945/1‑94 (MQ=60)
                                      GCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGC                                                        >  SRR4302535.4147432/1‑94 (MQ=60)
                                       CACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCT                                                       >  SRR4302535.4699401/1‑94 (MQ=60)
                                        ACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTT                                                      <  SRR4302535.932973/94‑1 (MQ=60)
                                        ACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTT                                                      <  SRR4302535.4404852/94‑1 (MQ=60)
                                         CGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTT                                                     <  SRR4302535.2515372/94‑1 (MQ=60)
                                          GCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTC                                                    <  SRR4302535.347805/94‑1 (MQ=60)
                                          GCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTC                                                    <  SRR4302535.84313/94‑1 (MQ=60)
                                           CCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCT                                                   >  SRR4302535.1969938/1‑94 (MQ=60)
                                           CCGTGCATCCCCAGCATACCCAGCGACAACGGATCCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCT                                                   <  SRR4302535.1041338/94‑1 (MQ=60)
                                            CGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTC                                                  >  SRR4302535.3296089/1‑94 (MQ=60)
                                            CGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGAAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTC                                                  >  SRR4302535.4516545/1‑94 (MQ=60)
                                                 ATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCA                                             <  SRR4302535.2734159/94‑1 (MQ=60)
                                                 ATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCA                                             >  SRR4302535.948434/1‑94 (MQ=60)
                                                   CCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGT                                           >  SRR4302535.2915303/1‑94 (MQ=60)
                                                   CCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGT                                           >  SRR4302535.3190807/1‑94 (MQ=60)
                                                   CCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGT                                           >  SRR4302535.3257771/1‑94 (MQ=60)
                                                       AGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCAC                                       >  SRR4302535.1156954/1‑94 (MQ=60)
                                                        GCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACG                                      >  SRR4302535.678630/1‑94 (MQ=60)
                                                          ATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCA                                    >  SRR4302535.4223173/1‑94 (MQ=60)
                                                          ATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCA                                    >  SRR4302535.1953239/1‑94 (MQ=60)
                                                          ATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCA                                    >  SRR4302535.177849/1‑94 (MQ=60)
                                                           TACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCAC                                   <  SRR4302535.961975/94‑1 (MQ=60)
                                                           TACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCAC                                   <  SRR4302535.1556405/94‑1 (MQ=60)
                                                             CCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCC                                 <  SRR4302535.4253261/94‑1 (MQ=60)
                                                             CCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCC                                 >  SRR4302535.2143158/1‑94 (MQ=60)
                                                                AGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTG                              >  SRR4302535.749462/1‑94 (MQ=60)
                                                                 GCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGC                             <  SRR4302535.865251/94‑1 (MQ=60)
                                                                  CGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCG                            <  SRR4302535.3754368/94‑1 (MQ=60)
                                                                   GACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGG                           >  SRR4302535.2306348/1‑94 (MQ=60)
                                                                    ACAACGGATGCGCTTTTGGTAACATACCCAGCGCCATTAAAGTCCTGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGG                          >  SRR4302535.1726443/1‑94 (MQ=60)
                                                                    ACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGG                          <  SRR4302535.2383724/94‑1 (MQ=60)
                                                                     CAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGG                         >  SRR4302535.2154919/1‑94 (MQ=60)
                                                                     CAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGC                         <  SRR4302535.381211/94‑1 (MQ=60)
                                                                      AACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCG                        >  SRR4302535.369636/1‑94 (MQ=60)
                                                                       ACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGC                       >  SRR4302535.57584/1‑94 (MQ=60)
                                                                       ACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGC                       >  SRR4302535.4572127/1‑94 (MQ=60)
                                                                        CGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTAGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCA                      >  SRR4302535.691608/1‑94 (MQ=60)
                                                                         GGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCAT                     <  SRR4302535.235256/94‑1 (MQ=60)
                                                                         GGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCAT                     >  SRR4302535.3947846/1‑94 (MQ=60)
                                                                         GGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCAT                     >  SRR4302535.4317765/1‑94 (MQ=60)
                                                                          GATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATT                    <  SRR4302535.1010369/94‑1 (MQ=60)
                                                                           ATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCATTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTG                   <  SRR4302535.3324145/94‑1 (MQ=60)
                                                                            TGCGCTTTTGGAAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGA                  >  SRR4302535.522163/1‑94 (MQ=60)
                                                                              CGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATC                <  SRR4302535.1690742/94‑1 (MQ=60)
                                                                              CGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATC                >  SRR4302535.753130/1‑94 (MQ=60)
                                                                              CGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATC                <  SRR4302535.364215/94‑1 (MQ=60)
                                                                               GCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCA               >  SRR4302535.3119932/1‑94 (MQ=60)
                                                                                CTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCAC              >  SRR4302535.1160800/1‑94 (MQ=60)
                                                                                CTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCAC              >  SRR4302535.926478/1‑94 (MQ=60)
                                                                                CTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCAC              <  SRR4302535.3917943/94‑1 (MQ=60)
                                                                                 TTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACA             <  SRR4302535.2986057/94‑1 (MQ=60)
                                                                                 TTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACA             <  SRR4302535.2189919/94‑1 (MQ=60)
                                                                                   TTGGCAACATACCCAGCGCCAGTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACC           <  SRR4302535.2337528/94‑1 (MQ=60)
                                                                                    TGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCG          >  SRR4302535.3212737/1‑94 (MQ=60)
                                                                                     GGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGC         >  SRR4302535.3878143/1‑94 (MQ=60)
                                                                                      GCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCC        <  SRR4302535.4158060/94‑1 (MQ=60)
                                                                                        AACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGC      >  SRR4302535.2262949/1‑94 (MQ=60)
                                                                                        AACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGC      >  SRR4302535.3654653/1‑94 (MQ=60)
                                                                                         ACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCC     >  SRR4302535.1765538/1‑94 (MQ=60)
                                                                                         ACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCC     <  SRR4302535.3128299/94‑1 (MQ=60)
                                                                                         ACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCC     <  SRR4302535.3968402/94‑1 (MQ=60)
                                                                                           ATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCCCA   >  SRR4302535.3233183/1‑94 (MQ=60)
                                                                                            TACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCCCAG  <  SRR4302535.2367449/94‑1 (MQ=60)
                                                                                            TACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCCCAG  >  SRR4302535.1171513/1‑94 (MQ=60)
                                                                                             |                                                                                            
CAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCCCAG  >  NC_000913/3849575‑3849760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑