Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,849,668 G→A G248G (GGC→GGT)  ilvB ← acetolactate synthase I large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,849,6680GA99.4% 1202.8 / ‑6.2 353G248G (GGC→GGT) ilvBacetolactate synthase I large subunit
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base A (216/135);  total (216/137)
Fisher's exact test for biased strand distribution p-value = 1.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 367 total aligned reads displayed.

CAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCC  >  NC_000913/3849578‑3849757
                                                                                          |                                                                                         
 aaaTCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATAccc                                                                                        >  2:304834/1‑93 (MQ=255)
    tCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAgc                                                                                     >  1:5627415/1‑93 (MQ=255)
    tCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGCGCATCCCCAGCATACCCAGCGACAGCGGACGAGCTTTTGGCAAGATACACAgc                                                                                     >  1:4181328/1‑93 (MQ=255)
      cGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAgcgc                                                                                   >  1:1237463/1‑93 (MQ=255)
      cGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAgcgc                                                                                   >  2:7460712/1‑93 (MQ=255)
       gCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCCGCGcc                                                                                  >  1:1012982/1‑93 (MQ=255)
       gCCTCCTGCAAAATATAGTCGGTGCTGCGCACGCCGTGCATCCCCAGCCTACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGcc                                                                                  >  1:504860/1‑93 (MQ=255)
        cctcctGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCa                                                                                 >  2:4339065/1‑93 (MQ=255)
          tcctGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCAtt                                                                               >  2:656791/1‑93 (MQ=255)
           cctGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTa                                                                              >  1:5847808/1‑93 (MQ=255)
             tGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGCTGCGCTTTTGTCAACATACCCAGCGCCATTaaa                                                                            >  1:7895477/1‑93 (MQ=255)
             tGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTaaa                                                                            <  2:359958/93‑1 (MQ=255)
             tGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTaaa                                                                            >  2:1462219/1‑93 (MQ=255)
                 aaaTATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCa                                                                        <  2:1122779/93‑1 (MQ=255)
                 aaaTATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCa                                                                        >  1:7617188/1‑93 (MQ=255)
                 aaaTATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCa                                                                        <  1:6179930/93‑1 (MQ=255)
                  aaTATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAt                                                                       <  1:141546/93‑1 (MQ=255)
                  aaTATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAt                                                                       <  1:464589/93‑1 (MQ=255)
                  aaTATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGAAACATACCCAGCGCCATTAAAGTCAt                                                                       >  1:2389686/1‑93 (MQ=255)
                   atatAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAtg                                                                      >  2:4897742/1‑93 (MQ=255)
                   atatAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAtg                                                                      >  2:5543467/1‑93 (MQ=255)
                    tataGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAtgg                                                                     >  2:5464124/1‑93 (MQ=255)
                    tataGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAtgg                                                                     >  2:4760808/1‑93 (MQ=255)
                       aGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAtggtgg                                                                  <  2:1985055/93‑1 (MQ=255)
                        gTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATggtggt                                                                 >  1:7570135/1‑93 (MQ=255)
                        gTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATggtggt                                                                 >  1:7643267/1‑93 (MQ=255)
                         ttGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGTAACATACCCAGCGCCATTAAAGTCATGGTGGTa                                                                >  2:1254256/1‑93 (MQ=255)
                           ggTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAgg                                                              >  2:5179239/1‑93 (MQ=255)
                           ggTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAgg                                                              >  2:7184606/1‑93 (MQ=255)
                             tgctgcGCACGCCGTGCATCCCCAGCATACCCAGCGACAACTGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCa                                                            <  1:1993439/93‑1 (MQ=255)
                              gctgcGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAg                                                           >  1:7934282/1‑93 (MQ=255)
                               ctgcGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGt                                                          >  1:2988625/1‑93 (MQ=255)
                                tgcGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGtt                                                         >  1:5967377/1‑93 (MQ=255)
                                tgcGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGtt                                                         <  2:1629752/93‑1 (MQ=255)
                                tgcGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGtt                                                         >  2:6709671/1‑93 (MQ=255)
                                tgcGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGACGCGCTTTTGGGAACATACCCAGCGCCAATAAAGTCATGGGGGGAGGccggt                                                         >  2:5694054/1‑89 (MQ=255)
                                     aCGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCt                                                    >  2:1022808/1‑93 (MQ=255)
                                        ccGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTtc                                                 <  1:6939046/93‑1 (MQ=255)
                                         cGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTtct                                                >  1:3582972/1‑93 (MQ=255)
                                          gTGCATCCCCAGCATACCCAGCGACAACTGATGCGCGTTTGGCAACAGACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTtctc                                               >  2:4737501/1‑93 (MQ=255)
                                          gTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTtctc                                               >  1:6767252/1‑93 (MQ=255)
                                          gTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTtctc                                               >  1:3482332/1‑93 (MQ=255)
                                             cATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGc                                            >  2:7697353/1‑93 (MQ=255)
                                              aTCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGcc                                           <  2:5057947/93‑1 (MQ=255)
                                               tCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCa                                          >  1:783283/1‑93 (MQ=255)
                                               tCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCa                                          >  1:2222114/1‑93 (MQ=255)
                                                ccccAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAg                                         <  2:2551735/93‑1 (MQ=255)
                                                 cccAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGt                                        >  1:47107/1‑93 (MQ=255)
                                                  ccAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGtt                                       >  1:23537/1‑93 (MQ=255)
                                                   cAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTc                                      >  2:1202564/1‑93 (MQ=255)
                                                     gCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCAc                                    >  2:5314490/1‑93 (MQ=255)
                                                     gCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCAc                                    <  1:2716834/93‑1 (MQ=255)
                                                     gCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCAc                                    <  2:2379068/93‑1 (MQ=255)
                                                      cATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACg                                   >  2:5936816/1‑93 (MQ=255)
                                                       aTACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGc                                  >  2:6858112/1‑93 (MQ=255)
                                                        tACCCAGCGACAACGGATGCGCTTTTGGTAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCa                                 >  2:2171508/1‑93 (MQ=255)
                                                        tACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCa                                 >  2:3916635/1‑93 (MQ=255)
                                                        tACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCa                                 >  1:6288758/1‑93 (MQ=255)
                                                        tACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCa                                 >  2:3371365/1‑93 (MQ=255)
                                                        tACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCCCCAGTTCACGcc                                 >  2:6969565/1‑92 (MQ=255)
                                                           ccAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCAccc                              >  1:1071456/1‑93 (MQ=255)
                                                           ccAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCAccc                              >  2:138446/1‑93 (MQ=255)
                                                            cAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCATTCTCCGCCAGTTCACGCACCCg                             >  2:4304640/1‑93 (MQ=255)
                                                            cAGCGACAACGGATGCGCTTTTGGCAACAACCCCAGCGCTATTAAAGTCATGGTGGTGGGCAGTTGCGCTTTCCCCGCCAGTTCACGCACCCg                             <  2:6313894/93‑1 (MQ=255)
                                                             aGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGt                            >  1:261887/1‑93 (MQ=255)
                                                               cGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGc                          <  1:522362/93‑1 (MQ=255)
                                                                gACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCg                         >  1:1859677/1‑93 (MQ=255)
                                                                gACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCg                         >  1:7064754/1‑93 (MQ=255)
                                                                gACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCg                         <  2:5977296/93‑1 (MQ=255)
                                                                gACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCg                         >  1:7270914/1‑93 (MQ=255)
                                                                 aCAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCgg                        <  1:6559468/93‑1 (MQ=255)
                                                                 aCAACGGATGCGCTTTTGGCAACATACCCAGCGGCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCAGCCAGGTCACGCACCCGTGCgg                        >  2:464938/1‑93 (MQ=255)
                                                                 aCAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCgg                        >  1:5368319/1‑93 (MQ=255)
                                                                 aCAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCgg                        <  1:905650/93‑1 (MQ=255)
                                                                   aaCGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGgc                      >  2:455163/1‑93 (MQ=255)
                                                                       gATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCAt                  >  2:4473944/1‑93 (MQ=255)
                                                                       gATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCAt                  >  1:4397045/1‑93 (MQ=255)
                                                                       gATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCAt                  >  2:5007162/1‑93 (MQ=255)
                                                                       gATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCAt                  <  2:3735319/93‑1 (MQ=255)
                                                                          gcgcTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGa               >  1:1455122/1‑93 (MQ=255)
                                                                            gcTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATc             <  2:3995805/93‑1 (MQ=255)
                                                                            gcTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATc             <  2:7303033/93‑1 (MQ=255)
                                                                            gcTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGCGCGGGCGCGTTGATc             >  1:2095071/1‑93 (MQ=255)
                                                                             cTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATca            >  1:5773216/1‑93 (MQ=255)
                                                                              ttttGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATcac           <  1:3831454/93‑1 (MQ=255)
                                                                              ttttGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATcac           >  2:6351167/1‑93 (MQ=255)
                                                                                ttGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCacac         <  1:3314388/93‑1 (MQ=255)
                                                                                ttGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCacac         <  1:3968841/93‑1 (MQ=255)
                                                                                ttGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCacac         <  2:2781917/93‑1 (MQ=255)
                                                                                ttGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCacac         <  2:6112916/93‑1 (MQ=255)
                                                                                ttGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCacac         <  1:4592940/93‑1 (MQ=255)
                                                                                 tGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACAcc        <  2:4113041/93‑1 (MQ=255)
                                                                                 tGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACAcc        <  2:2916248/93‑1 (MQ=255)
                                                                                  ggCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACAccg       <  2:1793597/93‑1 (MQ=255)
                                                                                  ggCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACAccg       <  2:2533602/93‑1 (MQ=255)
                                                                                    cAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACAccgcc     >  2:3825965/1‑93 (MQ=255)
                                                                                     aaCATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACAccgccg    <  2:2322237/93‑1 (MQ=255)
                                                                                     aaCATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACAccgccg    <  2:7514635/93‑1 (MQ=255)
                                                                                      aCATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACcgccgc   >  2:7811947/1‑93 (MQ=255)
                                                                                       cATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCgccgcc  <  2:7134968/93‑1 (MQ=255)
                                                                                          |                                                                                         
CAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCC  >  NC_000913/3849578‑3849757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCCCAGA  >  NC_000913/3849575‑3849761
                                                                                             |                                                                                             
CAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATA                                                                                               >  SRR4302543.1070041/1‑94 (MQ=60)
CAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATA                                                                                               >  SRR4302543.7477534/1‑94 (MQ=60)
CAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATA                                                                                               >  SRR4302543.4299198/1‑94 (MQ=60)
 AACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATAC                                                                                              >  SRR4302543.1304555/1‑94 (MQ=60)
  ACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACC                                                                                             <  SRR4302543.2280560/94‑1 (MQ=60)
   CAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCC                                                                                            >  SRR4302543.6718231/1‑94 (MQ=60)
     AATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAG                                                                                          >  SRR4302543.1246995/1‑94 (MQ=60)
      ATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGC                                                                                         >  SRR4302543.466205/1‑94 (MQ=60)
      ATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGC                                                                                         >  SRR4302543.5678693/1‑94 (MQ=60)
        CCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGC                                                                                       >  SRR4302543.1248598/1‑94 (MQ=60)
         CGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCCGCGCC                                                                                      >  SRR4302543.1021946/1‑94 (MQ=60)
         CGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCC                                                                                      >  SRR4302543.2301581/1‑94 (MQ=60)
         CGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCC                                                                                      >  SRR4302543.509552/1‑94 (MQ=60)
         CGCCTCCTGCAAAATATAGTCGGTGCTGCGCACGCCGTGCATCCCCAGCCTACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCC                                                                                      >  SRR4302543.509553/1‑94 (MQ=60)
           CCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCAT                                                                                    >  SRR4302543.216392/1‑94 (MQ=60)
           CCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCAT                                                                                    <  SRR4302543.5902312/94‑1 (MQ=60)
            CTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATT                                                                                   >  SRR4302543.4734437/1‑94 (MQ=60)
            CTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATT                                                                                   >  SRR4302543.3307278/1‑94 (MQ=60)
            CTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATT                                                                                   >  SRR4302543.232665/1‑94 (MQ=60)
            CTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATT                                                                                   >  SRR4302543.1282894/1‑94 (MQ=60)
            CTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGAAACATACCCAGCGCCATT                                                                                   >  SRR4302543.4734438/1‑94 (MQ=60)
             TCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTA                                                                                  <  SRR4302543.5378697/94‑1 (MQ=60)
             TCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTA                                                                                  >  SRR4302543.5901017/1‑94 (MQ=60)
              CCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAA                                                                                 >  SRR4302543.3016298/1‑94 (MQ=60)
              CCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAA                                                                                 >  SRR4302543.3016299/1‑94 (MQ=60)
               CTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGCTGCGCTTTTGTCAACATACCCAGCGCCATTAAA                                                                                >  SRR4302543.7969717/1‑94 (MQ=60)
               CTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAA                                                                                >  SRR4302543.7969715/1‑94 (MQ=60)
               CTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAA                                                                                >  SRR4302543.1939235/1‑94 (MQ=60)
               CTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAA                                                                                >  SRR4302543.4024687/1‑94 (MQ=60)
                TGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAG                                                                               <  SRR4302543.7198518/94‑1 (MQ=60)
                TGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAG                                                                               <  SRR4302543.3548949/94‑1 (MQ=60)
                TGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAG                                                                               <  SRR4302543.705806/94‑1 (MQ=60)
                  CAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTC                                                                             <  SRR4302543.5830974/94‑1 (MQ=60)
                  CAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTC                                                                             >  SRR4302543.3686955/1‑94 (MQ=60)
                   AAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCA                                                                            >  SRR4302543.6534153/1‑94 (MQ=60)
                   AAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCA                                                                            >  SRR4302543.7689492/1‑94 (MQ=60)
                    AAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAT                                                                           >  SRR4302543.86238/1‑94 (MQ=60)
                    AAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAT                                                                           <  SRR4302543.6238348/94‑1 (MQ=60)
                    AAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAT                                                                           <  SRR4302543.1362880/94‑1 (MQ=60)
                    AAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAT                                                                           <  SRR4302543.2609407/94‑1 (MQ=60)
                    AAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCAT                                                                           <  SRR4302543.1727558/94‑1 (MQ=60)
                    AAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGAAACATACCCAGCGCCATTAAAGTCAT                                                                           >  SRR4302543.2411315/1‑94 (MQ=60)
                     AATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATG                                                                          <  SRR4302543.468913/94‑1 (MQ=60)
                     AATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATG                                                                          <  SRR4302543.142782/94‑1 (MQ=60)
                     AATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATG                                                                          <  SRR4302543.198717/94‑1 (MQ=60)
                     AATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATG                                                                          <  SRR4302543.2404997/94‑1 (MQ=60)
                      ATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGG                                                                         >  SRR4302543.6249305/1‑94 (MQ=60)
                      ATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGG                                                                         >  SRR4302543.6913564/1‑94 (MQ=60)
                      ATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGG                                                                         >  SRR4302543.7404386/1‑94 (MQ=60)
                       TATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGT                                                                        <  SRR4302543.338096/94‑1 (MQ=60)
                       TATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGG                                                                        >  SRR4302543.2027631/1‑94 (MQ=60)
                         TAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGG                                                                      <  SRR4302543.396652/94‑1 (MQ=60)
                         TAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGG                                                                      >  SRR4302543.2556012/1‑94 (MQ=60)
                         TAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGG                                                                      <  SRR4302543.2696865/94‑1 (MQ=60)
                          AGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGT                                                                     >  SRR4302543.6401482/1‑94 (MQ=60)
                          AGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGT                                                                     >  SRR4302543.7715756/1‑94 (MQ=60)
                          AGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGT                                                                     >  SRR4302543.7642155/1‑94 (MQ=60)
                            TTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAG                                                                   <  SRR4302543.143926/94‑1 (MQ=60)
                            TTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAG                                                                   <  SRR4302543.5676853/94‑1 (MQ=60)
                            TTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAG                                                                   <  SRR4302543.5863043/94‑1 (MQ=60)
                             TGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTAGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGC                                                                  >  SRR4302543.1459594/1‑94 (MQ=60)
                               GTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCA                                                                <  SRR4302543.390674/94‑1 (MQ=60)
                               GTGCTGCGCACGCCGCGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCA                                                                <  SRR4302543.7954089/94‑1 (MQ=60)
                                TGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACTGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAG                                                               <  SRR4302543.2011312/94‑1 (MQ=60)
                                TGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAG                                                               >  SRR4302543.8008756/1‑94 (MQ=60)
                                TGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAG                                                               >  SRR4302543.709645/1‑94 (MQ=60)
                                 GCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGT                                                              >  SRR4302543.5777677/1‑94 (MQ=60)
                                 GCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGT                                                              >  SRR4302543.3015760/1‑94 (MQ=60)
                                  CTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTT                                                             >  SRR4302543.6022019/1‑94 (MQ=60)
                                     CGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCG                                                          <  SRR4302543.4528604/94‑1 (MQ=60)
                                         CGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTT                                                      >  SRR4302543.7440337/1‑94 (MQ=60)
                                          GCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTC                                                     <  SRR4302543.7704409/94‑1 (MQ=60)
                                           CCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCT                                                    >  SRR4302543.3614621/1‑94 (MQ=60)
                                           CCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCT                                                    >  SRR4302543.6560470/1‑94 (MQ=60)
                                           CCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCT                                                    >  SRR4302543.6623209/1‑94 (MQ=60)
                                           CCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCT                                                    <  SRR4302543.5941286/94‑1 (MQ=60)
                                           CCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCT                                                    <  SRR4302543.2223440/94‑1 (MQ=60)
                                           CCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCT                                                    <  SRR4302543.7004316/94‑1 (MQ=60)
                                            CGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTTCGCTTTCTC                                                   >  SRR4302543.7756878/1‑94 (MQ=60)
                                            CGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTC                                                   >  SRR4302543.6866996/1‑94 (MQ=60)
                                            CGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTC                                                   >  SRR4302543.3513522/1‑94 (MQ=60)
                                            CGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTC                                                   >  SRR4302543.6831160/1‑94 (MQ=60)
                                            CGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTC                                                   >  SRR4302543.1033658/1‑94 (MQ=60)
                                             GTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCC                                                  >  SRR4302543.6899629/1‑94 (MQ=60)
                                              TGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCG                                                 <  SRR4302543.4703738/94‑1 (MQ=60)
                                               GCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGC                                                >  SRR4302543.708468/1‑94 (MQ=60)
                                                CATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCC                                               >  SRR4302543.747118/1‑94 (MQ=60)
                                                CATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCC                                               >  SRR4302543.5672280/1‑94 (MQ=60)
                                                 ATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCA                                              >  SRR4302543.2242168/1‑94 (MQ=60)
                                                 ATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCA                                              >  SRR4302543.790176/1‑94 (MQ=60)
                                                   CCCCCGCATCCCCAGCGCCAACGGATGCCCTTTTGGCACCATACCCAGCCCCATTAAAGTCATGGTGGTACGCAGTTGCCCTTTCTCCGCCAGT                                            <  SRR4302543.45591/94‑1 (MQ=53)
                                                   CCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGT                                            >  SRR4302543.2737635/1‑94 (MQ=60)
                                                   CCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGT                                            <  SRR4302543.45400/94‑1 (MQ=60)
                                                   CCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGT                                            >  SRR4302543.47544/1‑94 (MQ=60)
                                                   CCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGACATGGTGGTAGGCAGTTGCGCTTTCTCTGCCAGC                                            >  SRR4302543.260575/1‑94 (MQ=60)
                                                    CCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTT                                           >  SRR4302543.23752/1‑94 (MQ=60)
                                                    CCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTT                                           >  SRR4302543.5454522/1‑94 (MQ=60)
                                                    CCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTT                                           >  SRR4302543.4034384/1‑94 (MQ=60)
                                                     CCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTC                                          >  SRR4302543.4666090/1‑94 (MQ=60)
                                                      CAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCA                                         <  SRR4302543.7655921/94‑1 (MQ=60)
                                                       AGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCAC                                        >  SRR4302543.5970100/1‑94 (MQ=60)
                                                       AGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCAC                                        >  SRR4302543.81099/1‑94 (MQ=60)
                                                        GCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACG                                       <  SRR4302543.2741521/94‑1 (MQ=60)
                                                        GCATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACG                                       >  SRR4302543.1469687/1‑94 (MQ=60)
                                                         CATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGC                                      >  SRR4302543.8006761/1‑94 (MQ=60)
                                                          ATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCA                                     >  SRR4302543.7643613/1‑94 (MQ=60)
                                                          ATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCA                                     >  SRR4302543.6056954/1‑94 (MQ=60)
                                                          ATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCA                                     >  SRR4302543.2881688/1‑94 (MQ=60)
                                                          ATACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCA                                     >  SRR4302543.6347985/1‑94 (MQ=60)
                                                          ATACCCAGCGACAACGGATGCGCTTTTGGAAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCA                                     >  SRR4302543.3506945/1‑94 (MQ=60)
                                                            ACCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACC                                   >  SRR4302543.2238931/1‑94 (MQ=60)
                                                             CCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCC                                  >  SRR4302543.1081088/1‑94 (MQ=60)
                                                             CCCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCC                                  >  SRR4302543.1081089/1‑94 (MQ=60)
                                                              CCAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCG                                 <  SRR4302543.2376366/94‑1 (MQ=60)
                                                               TAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGT                                >  SRR4302543.5715439/1‑94 (MQ=60)
                                                               CAGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGT                                >  SRR4302543.264178/1‑94 (MQ=60)
                                                                AGCGACAACGGATGCGCTTTTGGCGACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCATGCACCCGTG                               >  SRR4302543.5039607/1‑94 (MQ=60)
                                                                AGCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTG                               >  SRR4302543.5217907/1‑94 (MQ=60)
                                                                AGCGACAACGGATGCGCTTTTGGAAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGGGCTTTCTCCGCCAGTTCCGGCACCCGTG                               >  SRR4302543.5039809/1‑94 (MQ=60)
                                                                 GCGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGC                              <  SRR4302543.2291792/94‑1 (MQ=60)
                                                                  CGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCG                             >  SRR4302543.5246134/1‑94 (MQ=60)
                                                                  CGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCG                             >  SRR4302543.7133345/1‑94 (MQ=60)
                                                                  CGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCG                             >  SRR4302543.7133567/1‑94 (MQ=60)
                                                                  CGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCG                             >  SRR4302543.7341153/1‑94 (MQ=60)
                                                                  CGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCG                             >  SRR4302543.1876387/1‑94 (MQ=60)
                                                                  CGACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCG                             <  SRR4302543.527204/94‑1 (MQ=60)
                                                                   GACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAATTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGG                            <  SRR4302543.4562823/94‑1 (MQ=60)
                                                                   GACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGG                            >  SRR4302543.948388/1‑94 (MQ=60)
                                                                   GACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGG                            >  SRR4302543.5416919/1‑94 (MQ=60)
                                                                   GACAACGGATGCGCTTTTGGAAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGG                            >  SRR4302543.2741567/1‑94 (MQ=60)
                                                                    ACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGG                           <  SRR4302543.6621186/94‑1 (MQ=60)
                                                                    ACAACGGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGG                           <  SRR4302543.913623/94‑1 (MQ=60)
                                                                     CAACGGATGCGCTTTTTGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGC                          >  SRR4302543.108161/1‑94 (MQ=60)
                                                                         GGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCAT                      <  SRR4302543.954223/94‑1 (MQ=60)
                                                                         GGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCAT                      >  SRR4302543.4436907/1‑94 (MQ=60)
                                                                         GGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCAT                      >  SRR4302543.4307513/1‑94 (MQ=60)
                                                                         GGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCAT                      >  SRR4302543.3915818/1‑94 (MQ=60)
                                                                         GGATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCAT                      <  SRR4302543.358222/94‑1 (MQ=60)
                                                                          GATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATT                     >  SRR4302543.2662177/1‑94 (MQ=60)
                                                                          GATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATT                     >  SRR4302543.1232295/1‑94 (MQ=60)
                                                                           ATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTG                    >  SRR4302543.7711370/1‑94 (MQ=60)
                                                                           ATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTG                    >  SRR4302543.6334998/1‑94 (MQ=60)
                                                                           ATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTG                    <  SRR4302543.4428901/94‑1 (MQ=60)
                                                                           ATGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTG                    <  SRR4302543.4387941/94‑1 (MQ=60)
                                                                            TGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGA                   >  SRR4302543.1468255/1‑94 (MQ=60)
                                                                            TGCGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGA                   >  SRR4302543.6253448/1‑94 (MQ=60)
                                                                            TGCGCTTTTGGAAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGATCACGCACCCGTGCGGGCGCATTGA                   >  SRR4302543.3806143/1‑94 (MQ=60)
                                                                              CGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATC                 <  SRR4302543.7684134/94‑1 (MQ=60)
                                                                              CGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATC                 >  SRR4302543.2022803/1‑94 (MQ=60)
                                                                              CGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATC                 >  SRR4302543.1303556/1‑94 (MQ=60)
                                                                              CGCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGCGCGGGCGCGTTGATC                 >  SRR4302543.2113793/1‑94 (MQ=60)
                                                                               GCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCA                >  SRR4302543.5825768/1‑94 (MQ=60)
                                                                               GCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCA                >  SRR4302543.7755848/1‑94 (MQ=60)
                                                                               GCTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCA                >  SRR4302543.7883640/1‑94 (MQ=60)
                                                                                CTTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCAC               <  SRR4302543.818772/94‑1 (MQ=60)
                                                                                 TTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACA              <  SRR4302543.3865251/94‑1 (MQ=60)
                                                                                 TTTTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACA              >  SRR4302543.6152384/1‑94 (MQ=60)
                                                                                   TTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACC            <  SRR4302543.5293273/94‑1 (MQ=60)
                                                                                   TTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACC            <  SRR4302543.5302894/94‑1 (MQ=60)
                                                                                   TTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACC            <  SRR4302543.4004022/94‑1 (MQ=60)
                                                                                   TTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACC            <  SRR4302543.4634654/94‑1 (MQ=60)
                                                                                   TTGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACC            <  SRR4302543.3344302/94‑1 (MQ=60)
                                                                                    TGGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCG           >  SRR4302543.7943786/1‑94 (MQ=60)
                                                                                     GGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGC          >  SRR4302543.972155/1‑94 (MQ=60)
                                                                                     GGCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGC          <  SRR4302543.5842747/94‑1 (MQ=60)
                                                                                      GCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCC         >  SRR4302543.156379/1‑94 (MQ=60)
                                                                                      GCAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCC         <  SRR4302543.3734522/94‑1 (MQ=60)
                                                                                       CAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCG        >  SRR4302543.1914862/1‑94 (MQ=60)
                                                                                       CAACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCG        <  SRR4302543.3419809/94‑1 (MQ=60)
                                                                                        AACATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGC       >  SRR4302543.7970214/1‑94 (MQ=60)
                                                                                          CATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCCC     >  SRR4302543.5880678/1‑94 (MQ=60)
                                                                                           ATACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCCCA    >  SRR4302543.4992405/1‑94 (MQ=60)
                                                                                            TACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCCCAG   >  SRR4302543.229512/1‑94 (MQ=60)
                                                                                            TACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCCCAG   >  SRR4302543.3287239/1‑94 (MQ=60)
                                                                                            TACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCCCAG   <  SRR4302543.2215630/94‑1 (MQ=60)
                                                                                            TACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCCCAG   >  SRR4302543.7027225/1‑94 (MQ=60)
                                                                                             ACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCCCAGA  >  SRR4302543.7571937/1‑94 (MQ=60)
                                                                                             ACCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCCCAGA  >  SRR4302543.2657601/1‑94 (MQ=60)
                                                                                             |                                                                                             
CAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCCCAGA  >  NC_000913/3849575‑3849761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑