Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,860,862 T→C A123A (GCA→GCG)  b4553 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,860,8620TC100.0% 689.0 / NA 219A123A (GCA→GCG) b4553hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (118/101);  total (118/101)

Only 100 of 228 total aligned reads displayed.

AGCGCCGTCACAATGCCGGAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCTGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGGTCAGGAAAC  >  NC_000913/3860773‑3860950
                                                                                         |                                                                                        
aGCGCCGTCACAATGCCGGAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGcc                                                                                       >  2:2889458/1‑93 (MQ=255)
 gcgcCGTCACAATGCCGGAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCa                                                                                      >  1:4203278/1‑93 (MQ=255)
 gcgcCGTCACAATGCCGGAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCCCTACCCGccc                                                                                      >  2:169741/1‑92 (MQ=255)
  ctccGTCACAATGCCGGAAATGATAATCGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCCCCAc                                                                                     <  2:4457111/91‑1 (MQ=255)
     cGTCACAATGCCGGAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGGTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACcgc                                                                                  >  1:4612150/1‑93 (MQ=255)
       tCACAATGCCGGAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGt                                                                                >  1:2229853/1‑93 (MQ=255)
         acaATGCCGGAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTcc                                                                              >  1:4285016/1‑93 (MQ=255)
         acaATGCCGGAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTcc                                                                              >  1:3209489/1‑93 (MQ=255)
         acaATGCCGGAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTcc                                                                              >  1:3024897/1‑93 (MQ=255)
          caATGCCGGAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCt                                                                             <  1:1105940/93‑1 (MQ=255)
             tGCCGGAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAg                                                                          <  1:3953304/93‑1 (MQ=255)
               ccGGAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTg                                                                        >  2:1007881/1‑93 (MQ=255)
               ccGGAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTg                                                                        >  2:3979537/1‑93 (MQ=255)
                cGGAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGa                                                                       >  1:1087466/1‑93 (MQ=255)
                  gAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGaaa                                                                     <  1:3068601/93‑1 (MQ=255)
                   aaaTGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAAt                                                                    >  1:4648396/1‑93 (MQ=255)
                   aaaTGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAAt                                                                    <  2:1261203/93‑1 (MQ=255)
                   aaaTGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAAt                                                                    >  2:2200991/1‑93 (MQ=255)
                    aaTGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATc                                                                   <  2:541090/93‑1 (MQ=255)
                     aTGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCg                                                                  >  2:2006441/1‑93 (MQ=255)
                     aTGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCg                                                                  >  2:855520/1‑93 (MQ=255)
                     aTGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCg                                                                  >  2:4071454/1‑93 (MQ=255)
                      tGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGa                                                                 <  2:3876207/93‑1 (MQ=255)
                       gATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGag                                                                >  1:2093280/1‑92 (MQ=255)
                         tAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGc                                                              >  1:2171100/1‑93 (MQ=255)
                         tAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGc                                                              >  1:1368268/1‑93 (MQ=255)
                           aTTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCg                                                            >  2:2960870/1‑93 (MQ=255)
                            ttGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGa                                                           >  1:557793/1‑93 (MQ=255)
                            ttGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGa                                                           >  2:149807/1‑93 (MQ=255)
                            atGCGCCGATAATTCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGGTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGa                                                           <  2:3151987/92‑1 (MQ=255)
                             tGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGaa                                                          <  1:826912/93‑1 (MQ=255)
                             tGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGaa                                                          >  1:1999875/1‑93 (MQ=255)
                                gcCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTa                                                       <  2:3310999/93‑1 (MQ=255)
                                  cGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGc                                                     <  1:795632/93‑1 (MQ=255)
                                  cGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGc                                                     >  2:3392296/1‑93 (MQ=255)
                                   gATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCt                                                    >  1:1678100/1‑93 (MQ=255)
                                   gATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCt                                                    <  1:3228063/93‑1 (MQ=255)
                                    aTAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCtt                                                   >  2:970985/1‑93 (MQ=255)
                                      aaTGCTGGTATCGAGGGGTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTcc                                                 >  2:2481637/1‑93 (MQ=255)
                                       aTGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTccc                                                <  1:3181250/93‑1 (MQ=255)
                                        tGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTcccc                                               <  2:2712544/93‑1 (MQ=255)
                                          cTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAg                                             <  1:3747941/93‑1 (MQ=255)
                                           tGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAgt                                            >  1:3909046/1‑92 (MQ=255)
                                           tGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAgg                                            <  2:2781215/93‑1 (MQ=255)
                                           tGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAgg                                            >  1:430998/1‑93 (MQ=255)
                                            ggTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGt                                           >  1:1756870/1‑93 (MQ=255)
                                            ggTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGt                                           <  1:2807423/93‑1 (MQ=255)
                                              tATCGAGGGGTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCa                                         >  2:1124275/1‑93 (MQ=255)
                                                tCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCAtt                                       >  1:2057053/1‑93 (MQ=255)
                                                tCGAGGGGTTTAATACCGGCCATCATTGACAGAACGCTACTCCCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTTGTTTCCCCAGGTCAtt                                       >  2:1595137/1‑93 (MQ=255)
                                                  gAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTcc                                     <  2:3816718/93‑1 (MQ=255)
                                                   aGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTccc                                    >  1:3371879/1‑93 (MQ=255)
                                                   aGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTccc                                    >  2:3249917/1‑93 (MQ=255)
                                                    gggTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCa                                   >  2:4563684/1‑93 (MQ=255)
                                                     ggTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCAt                                  >  1:1035651/1‑93 (MQ=255)
                                                      gTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATc                                 >  1:4069605/1‑93 (MQ=255)
                                                      gTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATc                                 <  1:3631979/93‑1 (MQ=255)
                                                      gTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATc                                 >  2:1178942/1‑93 (MQ=255)
                                                         ttAATCCCGGCTATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATcgcg                              <  2:508579/93‑1 (MQ=255)
                                                          tAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGt                             >  2:153214/1‑93 (MQ=255)
                                                          tAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGt                             <  1:1565482/93‑1 (MQ=255)
                                                            aTCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTtg                           >  2:706066/1‑93 (MQ=255)
                                                             tCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTtga                          <  1:2362916/93‑1 (MQ=255)
                                                               ccGGCCACTATTCTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTtgatg                        <  1:339369/93‑1 (MQ=255)
                                                                cGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTtgatga                       <  2:127904/93‑1 (MQ=255)
                                                                cGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTtgatga                       >  2:2525156/1‑93 (MQ=255)
                                                                 ggCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGaa                      <  2:235967/93‑1 (MQ=255)
                                                                   ccATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAAt                    <  1:1657312/93‑1 (MQ=255)
                                                                    catcatTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATa                   <  2:1415462/93‑1 (MQ=255)
                                                                    catcatTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATa                   >  1:895898/1‑93 (MQ=255)
                                                                     atcatTGTCAGACCGCTACCCTCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAg                  <  1:4528657/93‑1 (MQ=255)
                                                                     atcatTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAg                  >  2:1307577/1‑93 (MQ=255)
                                                                     atcatTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAg                  >  1:1434218/1‑93 (MQ=255)
                                                                     atcatTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAg                  <  2:4275606/93‑1 (MQ=255)
                                                                      tcatTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGt                 <  2:2940359/93‑1 (MQ=255)
                                                                      tcatTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGt                 >  1:3776626/1‑93 (MQ=255)
                                                                      tcatTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGt                 <  1:2915181/93‑1 (MQ=255)
                                                                      tcatTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGGCATTCCCATCGCGTTGATGAAATAGt                 >  1:2572615/1‑93 (MQ=255)
                                                                       catTGTCAGGCCGCTCCCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGtt                <  1:1011016/93‑1 (MQ=255)
                                                                       catTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGtt                <  1:1238375/93‑1 (MQ=255)
                                                                       catTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGtt                >  1:1543847/1‑93 (MQ=255)
                                                                         ttGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTcc              >  2:3641985/1‑93 (MQ=255)
                                                                          tGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCa             <  1:3004692/93‑1 (MQ=255)
                                                                          tGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCa             <  1:4532564/93‑1 (MQ=255)
                                                                          tGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCa             <  1:3510406/93‑1 (MQ=255)
                                                                          tGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCa             <  1:246559/93‑1 (MQ=255)
                                                                          tGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCa             <  2:260992/93‑1 (MQ=255)
                                                                            tCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAgg           >  2:2110037/1‑93 (MQ=255)
                                                                            tCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAgg           <  2:2998508/93‑1 (MQ=255)
                                                                            tCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAgg           <  1:4084064/93‑1 (MQ=255)
                                                                             cAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGGt          <  1:1171662/93‑1 (MQ=255)
                                                                               gACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCGGGCCATTCCCATCGCGTTGATGAAAAAGTTCCAGGtag        >  2:4618927/1‑91 (MQ=255)
                                                                               gACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGGTGATGAAATAGTTCAAGGTcc        >  2:3228297/1‑92 (MQ=255)
                                                                                aCCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGGTCAg       <  1:3280246/93‑1 (MQ=255)
                                                                                 ccGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGGTCAgg      >  2:4007614/1‑93 (MQ=255)
                                                                                  cGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGGTCAGGa     <  1:4676729/93‑1 (MQ=255)
                                                                                   gCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGGTCAGGaa    >  2:2768144/1‑93 (MQ=255)
                                                                                     tACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGGTCAGGAAAc  >  1:108063/1‑93 (MQ=255)
                                                                                     tACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGGTCAGGAAAc  <  1:1823659/93‑1 (MQ=255)
                                                                                     caCCCGCCACCTCGTCCTGAGTAAACTCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGGTCAGGAAAc  <  2:3663577/92‑1 (MQ=255)
                                                                                         |                                                                                        
AGCGCCGTCACAATGCCGGAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCTGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGGTCAGGAAAC  >  NC_000913/3860773‑3860950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATGCAGCGCCGTCACAATGCCGGAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCTGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGGTCAGGAAACTCACC  >  NC_000913/3860769‑3860955
                                                                                             |                                                                                             
ATGCAGCGCCGTCACAATGCCGGAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCC                                                                                               >  SRR4302535.4607245/1‑94 (MQ=60)
   CAGCGCCGTCACAATGCCGGAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCC                                                                                            >  SRR4302535.1629236/1‑94 (MQ=60)
    AGCGCCGTCACAATGCCGGAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCA                                                                                           >  SRR4302535.4232042/1‑94 (MQ=60)
     GCGCCGTCACAATGCCGGAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGCCCGCTACCCGCCAC                                                                                          <  SRR4302535.4231840/94‑1 (MQ=60)
      CGCCGTCACAATGCCGGAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACC                                                                                         <  SRR4302535.829396/94‑1 (MQ=60)
       GCCGTCACAATGCCGGAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCG                                                                                        <  SRR4302535.3233664/94‑1 (MQ=60)
        CCGTCACAATGCCGGAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGC                                                                                       <  SRR4302535.559743/94‑1 (MQ=60)
        CCGTCACAATGCCGGAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGGTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGC                                                                                       >  SRR4302535.4643487/1‑94 (MQ=60)
         CGTCACAATGCCGGAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCG                                                                                      >  SRR4302535.2129115/1‑94 (MQ=60)
          GTCACAATGCCGGAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGT                                                                                     >  SRR4302535.2245709/1‑94 (MQ=60)
          GTCACAATGCCGGAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGATTTAATCCCGGCCATCATTGCCAGAACGCTACCCGCCACCGCGC                                                                                     >  SRR4302535.2018377/1‑94 (MQ=60)
            CACAATGCCGGAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCC                                                                                   >  SRR4302535.4314328/1‑94 (MQ=60)
            CACAATGCCGGAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCC                                                                                   >  SRR4302535.3231721/1‑94 (MQ=60)
            CACAATGCCGGAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCC                                                                                   >  SRR4302535.3046074/1‑94 (MQ=60)
              CAATGCCGGAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTG                                                                                 <  SRR4302535.1113841/94‑1 (MQ=60)
              CAATGCCGGAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTG                                                                                 <  SRR4302535.4250334/94‑1 (MQ=60)
              CAATGCCGGAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTG                                                                                 <  SRR4302535.1886758/94‑1 (MQ=60)
               AATGCCGGAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGA                                                                                >  SRR4302535.1539389/1‑94 (MQ=60)
                ATGCCGGAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAG                                                                               >  SRR4302535.4659688/1‑94 (MQ=60)
                ATGCCGGAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAG                                                                               <  SRR4302535.2288655/94‑1 (MQ=60)
                 TGCCGGAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGT                                                                              <  SRR4302535.3980293/94‑1 (MQ=60)
                   CCGGAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGA                                                                            >  SRR4302535.1095199/1‑94 (MQ=60)
                    CGGAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAA                                                                           <  SRR4302535.3045773/94‑1 (MQ=60)
                    CGGAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAA                                                                           >  SRR4302535.1687373/1‑94 (MQ=60)
                     GGAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAA                                                                          >  SRR4302535.2232788/1‑94 (MQ=60)
                     GGAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAA                                                                          >  SRR4302535.1561120/1‑94 (MQ=60)
                      GAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAAT                                                                         >  SRR4302535.3270191/1‑94 (MQ=60)
                      GAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAAT                                                                         <  SRR4302535.3090036/94‑1 (MQ=60)
                      GAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAAT                                                                         >  SRR4302535.4679880/1‑94 (MQ=60)
                          TGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGAG                                                                     >  SRR4302535.2108368/1‑94 (MQ=60)
                           GATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACG                                                                    <  SRR4302535.3427226/94‑1 (MQ=60)
                            ATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGC                                                                   >  SRR4302535.1803372/1‑94 (MQ=60)
                            ATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGC                                                                   <  SRR4302535.3009379/94‑1 (MQ=60)
                            ATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGC                                                                   >  SRR4302535.1378047/1‑94 (MQ=60)
                            ATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGC                                                                   >  SRR4302535.2186640/1‑94 (MQ=60)
                            ATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGC                                                                   >  SRR4302535.931203/1‑94 (MQ=60)
                             TAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCC                                                                  >  SRR4302535.1388369/1‑94 (MQ=60)
                              AATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCG                                                                 >  SRR4302535.3416845/1‑94 (MQ=60)
                               ATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGA                                                                >  SRR4302535.561698/1‑94 (MQ=60)
                                TTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAA                                                               >  SRR4302535.2014283/1‑94 (MQ=60)
                                 TGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAG                                                              <  SRR4302535.832701/94‑1 (MQ=60)
                                    GCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAG                                                           >  SRR4302535.1018785/1‑94 (MQ=60)
                                      CGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCT                                                         >  SRR4302535.1690250/1‑94 (MQ=60)
                                      CGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCT                                                         >  SRR4302535.3771822/1‑94 (MQ=60)
                                      CGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCT                                                         <  SRR4302535.801218/94‑1 (MQ=60)
                                       GATAATGCTGGTATCGAGGGTTTTACTCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGTTT                                                        <  SRR4302535.4561354/94‑1 (MQ=60)
                                       GATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTT                                                        <  SRR4302535.3250434/94‑1 (MQ=60)
                                         TAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCC                                                      <  SRR4302535.1848475/94‑1 (MQ=60)
                                          AATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCC                                                     >  SRR4302535.893756/1‑94 (MQ=60)
                                           ATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCC                                                    >  SRR4302535.170333/1‑94 (MQ=60)
                                           ATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCC                                                    <  SRR4302535.3203238/94‑1 (MQ=60)
                                              CTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGT                                                 >  SRR4302535.3935818/1‑94 (MQ=60)
                                              CTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGG                                                 >  SRR4302535.433999/1‑94 (MQ=60)
                                              CTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGG                                                 <  SRR4302535.3773869/94‑1 (MQ=60)
                                              CTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGG                                                 >  SRR4302535.379345/1‑94 (MQ=60)
                                               TGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGT                                                >  SRR4302535.1769572/1‑94 (MQ=60)
                                                GGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTC                                               <  SRR4302535.2827380/94‑1 (MQ=60)
                                                   ATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATT                                            >  SRR4302535.1259314/1‑94 (MQ=60)
                                                   ATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATT                                            >  SRR4302535.4629070/1‑94 (MQ=60)
                                                   ATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATT                                            >  SRR4302535.2071872/1‑94 (MQ=60)
                                                    TCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCGACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTC                                           <  SRR4302535.4336480/94‑1 (MQ=60)
                                                     CGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCC                                          >  SRR4302535.3167526/1‑94 (MQ=60)
                                                      GAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCC                                         >  SRR4302535.3395337/1‑94 (MQ=60)
                                                      GAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCC                                         >  SRR4302535.1241348/1‑94 (MQ=60)
                                                      GAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCC                                         >  SRR4302535.1094545/1‑94 (MQ=60)
                                                        GGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCAT                                       <  SRR4302535.3949812/94‑1 (MQ=60)
                                                        GGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCAT                                       >  SRR4302535.1043005/1‑94 (MQ=60)
                                                         GGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATC                                      >  SRR4302535.4097455/1‑94 (MQ=60)
                                                          GTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCG                                     >  SRR4302535.3224175/1‑94 (MQ=60)
                                                          GTTTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCG                                     <  SRR4302535.3657153/94‑1 (MQ=60)
                                                            TTTAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCG                                   >  SRR4302535.3225937/1‑94 (MQ=60)
                                                              TAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTT                                 <  SRR4302535.1576785/94‑1 (MQ=60)
                                                              TAATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTT                                 >  SRR4302535.1106564/1‑94 (MQ=60)
                                                               AATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTG                                <  SRR4302535.3398225/94‑1 (MQ=60)
                                                               AATCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTG                                <  SRR4302535.2044633/94‑1 (MQ=60)
                                                                 TCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGAT                              <  SRR4302535.1105298/94‑1 (MQ=60)
                                                                 TCCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGAT                              <  SRR4302535.2379887/94‑1 (MQ=60)
                                                                  CCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATG                             >  SRR4302535.4099256/1‑94 (MQ=60)
                                                                  CCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATG                             <  SRR4302535.2981729/94‑1 (MQ=60)
                                                                  CCCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGAGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCCAGTTGATG                             >  SRR4302535.1071285/1‑94 (MQ=60)
                                                                   CCGGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGA                            <  SRR4302535.2931610/94‑1 (MQ=60)
                                                                   CCGGCCACTATTCTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGA                            <  SRR4302535.341719/94‑1 (MQ=60)
                                                                     GGCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAA                          >  SRR4302535.1176906/1‑94 (MQ=60)
                                                                      GCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGATATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAAT                         >  SRR4302535.3316366/1‑94 (MQ=60)
                                                                      GCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAAT                         <  SRR4302535.1584462/94‑1 (MQ=60)
                                                                      GCCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAAT                         <  SRR4302535.3743750/94‑1 (MQ=60)
                                                                       CCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATA                        <  SRR4302535.4474527/94‑1 (MQ=60)
                                                                       CCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATA                        >  SRR4302535.902249/1‑94 (MQ=60)
                                                                       CCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATA                        <  SRR4302535.1669296/94‑1 (MQ=60)
                                                                       CCATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATA                        >  SRR4302535.3063640/1‑94 (MQ=60)
                                                                        CATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAG                       >  SRR4302535.4040666/1‑94 (MQ=60)
                                                                        CATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAG                       >  SRR4302535.993761/1‑94 (MQ=60)
                                                                        CATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAG                       >  SRR4302535.1444459/1‑94 (MQ=60)
                                                                         ATCATTGTCAGACCGCTACCCTCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGT                      <  SRR4302535.4559668/94‑1 (MQ=60)
                                                                         ATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGT                      >  SRR4302535.3802662/1‑94 (MQ=60)
                                                                         ATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGT                      >  SRR4302535.3542405/1‑94 (MQ=60)
                                                                         ATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGT                      <  SRR4302535.56407/94‑1 (MQ=60)
                                                                         ATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGT                      <  SRR4302535.2093655/94‑1 (MQ=60)
                                                                         ATCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGGCATTCCCATCGCGTTGATGAAATAGT                      >  SRR4302535.2591106/1‑94 (MQ=60)
                                                                          TCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTT                     >  SRR4302535.1554972/1‑94 (MQ=60)
                                                                          TCATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTT                     <  SRR4302535.2935767/94‑1 (MQ=60)
                                                                           CATTGTCAGGCCGCTCCCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTC                    <  SRR4302535.1018199/94‑1 (MQ=60)
                                                                           CATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTC                    <  SRR4302535.1247219/94‑1 (MQ=60)
                                                                           CATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTC                    <  SRR4302535.4249846/94‑1 (MQ=60)
                                                                            ATTGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCC                   >  SRR4302535.546528/1‑94 (MQ=60)
                                                                              TGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAG                 <  SRR4302535.3534798/94‑1 (MQ=60)
                                                                              TGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAG                 <  SRR4302535.3025752/94‑1 (MQ=60)
                                                                              TGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAG                 <  SRR4302535.4563587/94‑1 (MQ=60)
                                                                              TGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAG                 <  SRR4302535.248250/94‑1 (MQ=60)
                                                                              TGTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTAATTCCCATCGTGTTGATGAAATAGTTCCAG                 <  SRR4302535.4707320/94‑1 (MQ=60)
                                                                               GTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGG                <  SRR4302535.2125907/94‑1 (MQ=60)
                                                                               GTCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGG                <  SRR4302535.2508566/94‑1 (MQ=60)
                                                                                TCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGGT               <  SRR4302535.1397499/94‑1 (MQ=60)
                                                                                TCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGGT               <  SRR4302535.4111990/94‑1 (MQ=60)
                                                                                TCAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGGT               <  SRR4302535.386819/94‑1 (MQ=60)
                                                                                 CAGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGGTC              <  SRR4302535.1180028/94‑1 (MQ=60)
                                                                                  AGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGGTCA             <  SRR4302535.239712/94‑1 (MQ=60)
                                                                                  AGACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGGTCA             >  SRR4302535.2657604/1‑94 (MQ=60)
                                                                                   GACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGGTCAG            <  SRR4302535.4607637/94‑1 (MQ=60)
                                                                                    ACCGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGGTCAGG           <  SRR4302535.3303030/94‑1 (MQ=60)
                                                                                      CGCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGGTCAGGAA         <  SRR4302535.4708295/94‑1 (MQ=60)
                                                                                       GCTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGGTCAGGAAA        >  SRR4302535.2485893/1‑94 (MQ=60)
                                                                                        CTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGGTCAGGAAAC       >  SRR4302535.108828/1‑94 (MQ=60)
                                                                                        CTACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGGTCAGGAAAC       >  SRR4302535.3981815/1‑94 (MQ=60)
                                                                                         TACCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGGTCAGGAAACT      <  SRR4302535.1836828/94‑1 (MQ=60)
                                                                                           CCCGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGGTCAGGAAACTCA    >  SRR4302535.1806857/1‑94 (MQ=60)
                                                                                             CGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGGTCAGGAAACTCACC  >  SRR4302535.2676726/1‑94 (MQ=60)
                                                                                             CGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGGTCAGGAAACTCACC  >  SRR4302535.3195489/1‑94 (MQ=60)
                                                                                             |                                                                                             
ATGCAGCGCCGTCACAATGCCGGAAATGATAATTGCGCCGATAATGCTGGTATCGAGGGTTTTAATCCCGGCCATCATTGTCAGACCGCTACCTGCCACCGCGTCCTGAGTGAAATCGACGCCGAAGTAGCTTCCCCAGGTCATTCCCATCGCGTTGATGAAATAGTTCCAGGTCAGGAAACTCACC  >  NC_000913/3860769‑3860955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑