Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,872,450 +G coding (338/387 nt) yidW ← regulator protein for dgo operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,872,4501.G99.6% 807.7 / NA 253E113A (GAG→GCG) yidWregulator protein for dgo operon
Reads supporting (aligned to +/- strand):  ref base . (0/0);  major base G (118/134);  minor base C (0/1);  total (118/135)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 271 total aligned reads displayed.

TCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCT‑CCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACC  >  NC_000913/3872358‑3872536
                                                                                             |                                                                                      
  gTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCtgc                                                                                       <  2:2848843/93‑3 (MQ=255)
    tAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCT‑gccc                                                                                    >  2:2968507/1‑92 (MQ=255)
     aaaCGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCT‑gccca                                                                                   >  2:2468339/1‑91 (MQ=255)
     aaaCGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCT‑gccca                                                                                   >  2:4381739/1‑91 (MQ=255)
      aaCGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCaa                                                                                   >  1:3059575/1‑93 (MQ=255)
       aCGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAAc                                                                                  >  1:207568/1‑93 (MQ=255)
       aCGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGACGCGCGCTCTGCTGCCCAAc                                                                                  <  2:4511793/93‑1 (MQ=255)
          cTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCCACggg                                                                               >  1:946269/1‑93 (MQ=255)
            tCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCa                                                                             <  1:1350871/93‑1 (MQ=255)
             cgcgGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCaa                                                                            <  2:3759773/93‑1 (MQ=255)
             cgcgGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCaa                                                                            <  2:60137/93‑1 (MQ=255)
             cgcgGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCaa                                                                            <  2:3370611/93‑1 (MQ=255)
               cgGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATc                                                                          <  2:1114638/93‑1 (MQ=255)
               cgGTCCTGATTGTTGGCAATCATCTCGTGCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATc                                                                          <  1:1245814/93‑1 (MQ=255)
                 gTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGc                                                                        <  1:708158/93‑1 (MQ=255)
                 gTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGc                                                                        <  2:3880041/93‑1 (MQ=255)
                   ccTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCg                                                                      >  2:2065690/1‑93 (MQ=255)
                    cTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCgg                                                                     >  2:3620532/1‑93 (MQ=255)
                     tGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGt                                                                    >  1:3648473/1‑93 (MQ=255)
                       aTTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTc                                                                  >  2:2025390/1‑93 (MQ=255)
                       aTTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTc                                                                  >  1:1405693/1‑93 (MQ=255)
                        ttgttgGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTcc                                                                 <  2:4169042/93‑1 (MQ=255)
                        ttgttgGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTcc                                                                 <  1:1532954/93‑1 (MQ=255)
                           ttgGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTccacc                                                              <  2:2943125/93‑1 (MQ=255)
                           ttgGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTccacc                                                              >  2:214615/1‑93 (MQ=255)
                            tgGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTccacca                                                             <  1:3662464/93‑1 (MQ=255)
                              gCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGa                                                           <  2:1290265/93‑1 (MQ=255)
                              gCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGa                                                           <  1:3956967/93‑1 (MQ=255)
                              gCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGa                                                           <  2:1976488/93‑1 (MQ=255)
                                aaTCATCTTGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGAtt                                                         >  1:3738943/1‑93 (MQ=255)
                                 atcatcTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGAttt                                                        >  2:4663171/1‑93 (MQ=255)
                                 atcatcTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGAttt                                                        <  1:27996/93‑1 (MQ=255)
                                  tcatcTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTc                                                       >  2:1627198/1‑93 (MQ=255)
                                  tcatcTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTc                                                       <  1:4573383/93‑1 (MQ=255)
                                     tctcGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCa                                                    <  2:4331036/93‑1 (MQ=255)
                                     tctcGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCa                                                    <  1:3510178/93‑1 (MQ=255)
                                     tctcGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCa                                                    >  2:4605770/1‑93 (MQ=255)
                                     tctcGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCa                                                    <  2:3509836/93‑1 (MQ=255)
                                     tctcGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCa                                                    <  1:2203423/93‑1 (MQ=255)
                                      ctcGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCAc                                                   <  1:4359129/93‑1 (MQ=255)
                                       tcGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACt                                                  >  2:2170129/1‑93 (MQ=255)
                                        cGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACtt                                                 >  2:4235049/1‑93 (MQ=255)
                                        cGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACtt                                                 <  2:1316876/93‑1 (MQ=255)
                                         gTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTc                                                <  2:4139714/93‑1 (MQ=255)
                                         gTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTc                                                <  1:3445447/93‑1 (MQ=255)
                                         gTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTCCCCAACGGTCAATCGCCGGTTCCACCAGATTTCGCACTTc                                                <  1:4304787/93‑1 (MQ=255)
                                          ttCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCg                                               >  2:3411518/1‑93 (MQ=255)
                                           tCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGc                                              >  1:4606893/1‑93 (MQ=255)
                                              gcgcCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCa                                           >  1:869655/1‑93 (MQ=255)
                                               cgcCGATTCAATCTGCGCCAGCTCGCTGGACGTCGCGCGCTCTTCTGCCTACCGGGCAATCGCCGGTTCCACCAAATTTCGCGCTTCGCTCAt                                          <  2:4366519/93‑1 (MQ=255)
                                               cgcCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCAt                                          >  1:2157558/1‑93 (MQ=255)
                                                gcCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATg                                         >  1:99476/1‑93 (MQ=255)
                                                gcCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATg                                         <  1:3015892/93‑1 (MQ=255)
                                                  cGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGc                                       >  1:1031621/1‑93 (MQ=255)
                                                   gATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCa                                      >  2:3100066/1‑93 (MQ=255)
                                                   gATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCa                                      >  2:3795626/1‑93 (MQ=255)
                                                    aTTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCAc                                     <  1:1971299/93‑1 (MQ=255)
                                                     ttCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACt                                    >  2:1712968/1‑93 (MQ=255)
                                                     ttCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACt                                    >  2:3966784/1‑93 (MQ=255)
                                                     ttCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACt                                    <  2:706776/93‑1 (MQ=255)
                                                       cAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGa                                  <  1:3819454/93‑1 (MQ=255)
                                                        aaTCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGAt                                 >  2:2287752/1‑93 (MQ=255)
                                                        aaTCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGAt                                 >  2:2551335/1‑93 (MQ=255)
                                                         aTCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATa                                >  1:1887557/1‑93 (MQ=255)
                                                          tCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATaa                               >  2:2686678/1‑93 (MQ=255)
                                                          tCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATaa                               <  1:1459943/93‑1 (MQ=255)
                                                            tGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGc                             <  2:2407402/93‑1 (MQ=255)
                                                            tGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGc                             <  2:3020526/93‑1 (MQ=255)
                                                             gcgcCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGcc                            <  1:3418028/93‑1 (MQ=255)
                                                             gcgcCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGcc                            <  1:2393941/93‑1 (MQ=255)
                                                               gcCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGt                          >  2:1454702/1‑93 (MQ=255)
                                                                ccAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTg                         <  1:2052385/93‑1 (MQ=255)
                                                                 cAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTgg                        <  2:1518089/93‑1 (MQ=255)
                                                                  aGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTggg                       >  1:3890051/1‑93 (MQ=255)
                                                                  aGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTggg                       >  1:219766/1‑93 (MQ=255)
                                                                   gATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGt                      >  1:3822656/1‑93 (MQ=255)
                                                                   gATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGt                      <  2:3137794/93‑1 (MQ=255)
                                                                    aTCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTc                     >  1:1655001/1‑93 (MQ=255)
                                                                    aTCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTc                     <  1:2563426/93‑1 (MQ=255)
                                                                     tCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCg                    >  2:18609/1‑93 (MQ=255)
                                                                      cGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGt                   >  2:4036292/1‑93 (MQ=255)
                                                                       gCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTa                  <  1:359502/93‑1 (MQ=255)
                                                                       gCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGAAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTa                  >  2:1183888/1‑93 (MQ=255)
                                                                        cTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAg                 >  1:768702/1‑93 (MQ=255)
                                                                         tGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGt                <  1:2922160/93‑1 (MQ=255)
                                                                         tGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGt                >  1:2792835/1‑93 (MQ=255)
                                                                          ggAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTc               >  2:537134/1‑93 (MQ=255)
                                                                             aGTCGCGCTCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCAtt            <  2:2804040/93‑1 (MQ=255)
                                                                             aGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCAtt            >  1:3264809/1‑93 (MQ=255)
                                                                             aGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCAtt            <  2:625101/93‑1 (MQ=255)
                                                                             aGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCAtt            >  2:1947643/1‑93 (MQ=255)
                                                                              ggcgcgcgCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCAttt           <  2:330369/91‑1 (MQ=255)
                                                                              gTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCAttt           <  1:1107055/93‑1 (MQ=255)
                                                                              gTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCAttt           <  2:3261575/93‑1 (MQ=255)
                                                                                cgcgcgCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTc         <  2:968012/93‑1 (MQ=255)
                                                                                 gcgcgcTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTcc        >  2:782135/1‑93 (MQ=255)
                                                                                     gcTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCa    >  2:891804/1‑93 (MQ=255)
                                                                                      ctctGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCAc   >  2:1474241/1‑93 (MQ=255)
                                                                                       tctGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCAcc  <  1:4057200/93‑1 (MQ=255)
                                                                                       tctGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTAATTTTCCAGCAcc  >  2:742316/1‑93 (MQ=255)
                                                                                             |                                                                                      
TCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCT‑CCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACC  >  NC_000913/3872358‑3872536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCT‑CCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCA  >  NC_000913/3872357‑3872543
                                                                                             |                                                                                              
TTCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCT                                                                                                <  SRR4302535.4333133/94‑1 (MQ=60)
  CGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCT‑GC                                                                                             >  SRR4302535.3774900/1‑94 (MQ=60)
     TAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCA                                                                                           <  SRR4302535.3701551/94‑1 (MQ=60)
      AAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAA                                                                                          >  SRR4302535.3080975/1‑94 (MQ=60)
       AACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAAC                                                                                         >  SRR4302535.208993/1‑94 (MQ=60)
        ACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACG                                                                                        <  SRR4302535.3633294/94‑1 (MQ=60)
         CGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGG                                                                                       <  SRR4302535.10002/94‑1 (MQ=60)
         CGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGG                                                                                       <  SRR4302535.4180987/94‑1 (MQ=60)
          GCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCCACGGG                                                                                      >  SRR4302535.952974/1‑94 (MQ=60)
          GCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGG                                                                                      <  SRR4302535.3553405/94‑1 (MQ=60)
          GCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGG                                                                                      >  SRR4302535.2985812/1‑94 (MQ=60)
             TCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAA                                                                                   <  SRR4302535.1360538/94‑1 (MQ=60)
              CGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAAT                                                                                  <  SRR4302535.3501426/94‑1 (MQ=60)
              CGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAAT                                                                                  <  SRR4302535.3622583/94‑1 (MQ=60)
               GCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATC                                                                                 <  SRR4302535.786313/94‑1 (MQ=60)
                CGGTCCTGATTGTTGGCAATCATCTCGTGCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCG                                                                                <  SRR4302535.1254703/94‑1 (MQ=60)
                  GTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCC                                                                              <  SRR4302535.127065/94‑1 (MQ=60)
                  GTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCC                                                                              <  SRR4302535.713127/94‑1 (MQ=60)
                   TCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCG                                                                             <  SRR4302535.2845627/94‑1 (MQ=60)
                   TCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCG                                                                             <  SRR4302535.1426648/94‑1 (MQ=60)
                    CCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGG                                                                            >  SRR4302535.143520/1‑94 (MQ=60)
                     CTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGT                                                                           >  SRR4302535.1384006/1‑94 (MQ=60)
                     CTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGT                                                                           >  SRR4302535.3673733/1‑94 (MQ=60)
                      TGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTT                                                                          >  SRR4302535.3124931/1‑94 (MQ=60)
                      TGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTT                                                                          <  SRR4302535.1980707/94‑1 (MQ=60)
                       GATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTC                                                                         >  SRR4302535.1415739/1‑94 (MQ=60)
                       GATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTC                                                                         >  SRR4302535.745628/1‑94 (MQ=60)
                        ATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCC                                                                        >  SRR4302535.991189/1‑94 (MQ=60)
                        ATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCC                                                                        >  SRR4302535.1631049/1‑94 (MQ=60)
                         TTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCA                                                                       <  SRR4302535.1543998/94‑1 (MQ=60)
                             TGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAG                                                                   <  SRR4302535.1539576/94‑1 (MQ=60)
                             TGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAG                                                                   <  SRR4302535.3687824/94‑1 (MQ=60)
                              GGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGA                                                                  >  SRR4302535.1250725/1‑94 (MQ=60)
                               GCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGAT                                                                 <  SRR4302535.3983983/94‑1 (MQ=60)
                                CAATCATCTTGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATT                                                                >  SRR4302535.3764820/1‑94 (MQ=60)
                                CAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATT                                                                >  SRR4302535.3468605/1‑94 (MQ=60)
                                 AATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTT                                                               <  SRR4302535.1813236/94‑1 (MQ=60)
                                  ATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTC                                                              <  SRR4302535.3858846/94‑1 (MQ=60)
                                  ATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTC                                                              >  SRR4302535.1902771/1‑94 (MQ=60)
                                  ATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTC                                                              <  SRR4302535.28217/94‑1 (MQ=60)
                                   TCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCG                                                             <  SRR4302535.4604558/94‑1 (MQ=60)
                                     ATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCC                                                           >  SRR4302535.3785734/1‑94 (MQ=60)
                                     ATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCA                                                           >  SRR4302535.4040612/1‑94 (MQ=60)
                                      TCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCAC                                                          <  SRR4302535.2219124/94‑1 (MQ=60)
                                      TCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCAC                                                          <  SRR4302535.816546/94‑1 (MQ=60)
                                      TCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCAC                                                          <  SRR4302535.523050/94‑1 (MQ=60)
                                      TCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCAC                                                          <  SRR4302535.3534570/94‑1 (MQ=60)
                                       CTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACT                                                         <  SRR4302535.39683/94‑1 (MQ=60)
                                       CTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACT                                                         <  SRR4302535.4388968/94‑1 (MQ=60)
                                        TCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTT                                                        <  SRR4302535.4435639/94‑1 (MQ=60)
                                          GTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCG                                                      <  SRR4302535.3730236/94‑1 (MQ=60)
                                          GTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCG                                                      <  SRR4302535.3330578/94‑1 (MQ=60)
                                          GTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCG                                                      <  SRR4302535.3469403/94‑1 (MQ=60)
                                          GTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTCCCCAACGGTCAATCGCCGGTTCCACCAGATTTCGCACTTCG                                                      <  SRR4302535.4334239/94‑1 (MQ=60)
                                           TTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGC                                                     >  SRR4302535.4630268/1‑94 (MQ=60)
                                           TTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGC                                                     >  SRR4302535.4638201/1‑94 (MQ=60)
                                            TCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCT                                                    >  SRR4302535.4008399/1‑94 (MQ=60)
                                              AGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCA                                                  >  SRR4302535.875817/1‑94 (MQ=60)
                                               GCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCAT                                                 >  SRR4302535.2173066/1‑94 (MQ=60)
                                               GCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCAT                                                 >  SRR4302535.1879432/1‑94 (MQ=60)
                                                CGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATG                                                >  SRR4302535.100176/1‑94 (MQ=60)
                                                CGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATG                                                <  SRR4302535.2851185/94‑1 (MQ=60)
                                                 GCCGATTCAATCTGCTCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGG                                               <  SRR4302535.2084000/94‑1 (MQ=60)
                                                 GCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGG                                               <  SRR4302535.3037025/94‑1 (MQ=60)
                                                  CCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGC                                              >  SRR4302535.1038948/1‑94 (MQ=60)
                                                  CCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGC                                              >  SRR4302535.877594/1‑94 (MQ=60)
                                                   CGATTCAATCTGCGCCAGATCGCTCGAAGTCGCGCGCTCTGCTGCCCAATGGCGAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCC                                             >  SRR4302535.1123794/1‑94 (MQ=60)
                                                     ATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACT                                           <  SRR4302535.1985496/94‑1 (MQ=60)
                                                     ATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACT                                           <  SRR4302535.2164378/94‑1 (MQ=60)
                                                        CAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGAT                                        <  SRR4302535.4440530/94‑1 (MQ=60)
                                                        CAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGAT                                        <  SRR4302535.3845810/94‑1 (MQ=60)
                                                        CAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGAT                                        <  SRR4302535.2818595/94‑1 (MQ=60)
                                                         AATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATA                                       >  SRR4302535.1901154/1‑94 (MQ=60)
                                                          ATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAA                                      <  SRR4302535.4677313/94‑1 (MQ=60)
                                                           TCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAG                                     <  SRR4302535.2168341/94‑1 (MQ=60)
                                                           TCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAG                                     <  SRR4302535.1470402/94‑1 (MQ=60)
                                                             TGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCC                                   <  SRR4302535.3261597/94‑1 (MQ=60)
                                                              GCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCG                                  <  SRR4302535.2411150/94‑1 (MQ=60)
                                                              GCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCG                                  <  SRR4302535.4341454/94‑1 (MQ=60)
                                                              GCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCG                                  <  SRR4302535.3441806/94‑1 (MQ=60)
                                                               CGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGT                                 <  SRR4302535.2207623/94‑1 (MQ=60)
                                                                GCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTG                                <  SRR4302535.2195765/94‑1 (MQ=60)
                                                                GCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTG                                <  SRR4302535.1266005/94‑1 (MQ=60)
                                                                 CCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGG                               <  SRR4302535.3847927/94‑1 (MQ=60)
                                                                 CCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGG                               <  SRR4302535.2067161/94‑1 (MQ=60)
                                                                 CCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGG                               <  SRR4302535.3396332/94‑1 (MQ=60)
                                                                 CCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGG                               >  SRR4302535.3467119/1‑94 (MQ=60)
                                                                  CAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGG                              >  SRR4302535.221279/1‑94 (MQ=60)
                                                                  CAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGG                              >  SRR4302535.3916722/1‑94 (MQ=60)
                                                                  CAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGG                              >  SRR4302535.4340474/1‑94 (MQ=60)
                                                                   AGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGT                             >  SRR4302535.3849024/1‑94 (MQ=60)
                                                                    GATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTC                            >  SRR4302535.121140/1‑94 (MQ=60)
                                                                    GATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTC                            >  SRR4302535.2738573/1‑94 (MQ=60)
                                                                    GATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTC                            >  SRR4302535.1666972/1‑94 (MQ=60)
                                                                     ATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCG                           <  SRR4302535.2581877/94‑1 (MQ=60)
                                                                     ATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCG                           <  SRR4302535.1525006/94‑1 (MQ=60)
                                                                        GCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAG                        <  SRR4302535.361991/94‑1 (MQ=60)
                                                                        GCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAG                        >  SRR4302535.774112/1‑94 (MQ=60)
                                                                         CTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGT                       >  SRR4302535.2983482/1‑94 (MQ=60)
                                                                         CTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGT                       >  SRR4302535.2812708/1‑94 (MQ=60)
                                                                         CTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGT                       <  SRR4302535.2552794/94‑1 (MQ=60)
                                                                          TGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTC                      >  SRR4302535.1063247/1‑94 (MQ=60)
                                                                          TGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTC                      <  SRR4302535.2942771/94‑1 (MQ=60)
                                                                           GGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCA                     <  SRR4302535.3677204/94‑1 (MQ=60)
                                                                           GGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCA                     >  SRR4302535.2704329/1‑94 (MQ=60)
                                                                            GAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCAT                    <  SRR4302535.2278971/94‑1 (MQ=60)
                                                                            GAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCAT                    >  SRR4302535.3846876/1‑94 (MQ=60)
                                                                             AAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATT                   >  SRR4302535.3287485/1‑94 (MQ=60)
                                                                              AGTCGCGCGCTCTGCTGCCCAACGGTCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGTGTCGTCGTCATTT                  >  SRR4302535.3585432/1‑94 (MQ=60)
                                                                               GTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTT                 <  SRR4302535.1114963/94‑1 (MQ=60)
                                                                               GTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTT                 <  SRR4302535.1997397/94‑1 (MQ=60)
                                                                                TCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTC                <  SRR4302535.75045/94‑1 (MQ=60)
                                                                                 CGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCC               >  SRR4302535.3922816/1‑94 (MQ=60)
                                                                                 CGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCC               >  SRR4302535.3041233/1‑94 (MQ=60)
                                                                                 AGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCC               <  SRR4302535.2542872/94‑1 (MQ=60)
                                                                                    GCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGC            <  SRR4302535.3581617/94‑1 (MQ=60)
                                                                                      GCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCAC          >  SRR4302535.204419/1‑94 (MQ=60)
                                                                                       CTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACC         <  SRR4302535.2218552/94‑1 (MQ=60)
                                                                                        TCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACCC        <  SRR4302535.226885/94‑1 (MQ=60)
                                                                                        TCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACCC        <  SRR4302535.4084972/94‑1 (MQ=60)
                                                                                         CTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACCCA       >  SRR4302535.1369514/1‑94 (MQ=60)
                                                                                         CTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACCCA       >  SRR4302535.3285431/1‑94 (MQ=60)
                                                                                          TGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACCCAT      >  SRR4302535.2217747/1‑94 (MQ=60)
                                                                                          TGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACCCAT      >  SRR4302535.1496622/1‑94 (MQ=60)
                                                                                          TGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACCCAT      <  SRR4302535.4218583/94‑1 (MQ=60)
                                                                                           GCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACCCATT     >  SRR4302535.1893073/1‑94 (MQ=60)
                                                                                           GCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACCCATT     <  SRR4302535.1256024/94‑1 (MQ=60)
                                                                                             G‑CCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCA  <  SRR4302535.3494960/94‑1 (MQ=60)
                                                                                             |                                                                                              
TTCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCT‑CCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCA  >  NC_000913/3872357‑3872543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑