Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,874,663	(G)_5→6	intergenic (‑83/+32)	yidA ← / ← b3698	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,874,658	1	.	G	99.5%	614.4 / ‑3.9	197	intergenic (‑78/+37)	yidA/b3698	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (103/93); total (104/93)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.52e-01

Only 100 of 258 total aligned reads displayed.

GAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAA‑GG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAGATCGT  >  NC_000913/3874566‑3874747

gAGTTTAATAGCCGTGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGa‑aaa                                                                                              >  1:3269368/1‑93 (MQ=255)

gAGTTTAATAGCCGTGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGa‑aaa                                                                                              <  2:3166024/93‑1 (MQ=255)

gAGTTTAATAGCCGTGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGa‑aaa                                                                                              <  1:1447362/93‑1 (MQ=255)

gAGTTTAATAGCCGTGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGa‑aaa                                                                                              >  1:1043723/1‑93 (MQ=255)

 aGTTTAATAGCCGTGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAA‑g                                                                                            >  2:3402388/1‑93 (MQ=255)

  gTTTAATAGCCGTGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAA‑gg                                                                                           >  2:4484714/1‑93 (MQ=255)

  gTTTAATAGCCGTGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAA‑gg                                                                                           <  1:112856/93‑1 (MQ=255)

   tttAATAGCCGTGAGTTCCCCTGGTAGATTGGTTACTGACCCATGCTAACGCGATTCCGCTCATAAATTCGTCGAACACCCGCGGGG‑AAA‑gg‑g                                                                                         >  2:3961012/1‑93 (MQ=255)

   tttAATAGCCGTGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAA‑gg‑g                                                                                         >  1:989609/1‑93 (MQ=255)

   tttAATAGCCGTGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAA‑gg‑g                                                                                         <  1:3843084/93‑1 (MQ=255)

     tAATAGCCGTGAGTTCCCCAGGTAGATTGGTTCCTGGCCCATGCTAACGCGAATCCGCTCAAAAACCAGGACAACCCCCCGGGGA‑AAA‑gg‑gg‑g                                                                                      <  2:1618510/93‑1 (MQ=255)

     tAATAGCCGTGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAA‑gg‑gg‑g                                                                                      <  1:1076743/93‑1 (MQ=255)

      aaTAGCCGTGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAA‑gg‑gg‑gg                                                                                     <  1:39824/93‑2 (MQ=255)

        tAGCCGTGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAA‑gg‑gg‑ggat                                                                                   <  1:4492010/93‑4 (MQ=255)

        tAGCCGTGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAA‑gg‑gg‑ggag                                                                                   >  1:4114021/1‑90 (MQ=255)

         aGCCGTGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAA‑gg‑gg‑ggatg                                                                                  <  2:1395067/93‑5 (MQ=255)

            cgtGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCaa                                                                                >  1:2818538/3‑93 (MQ=255)

             gtGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAAc                                                                               >  2:4078732/2‑93 (MQ=255)

             gtGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAAc                                                                               >  1:3585459/2‑93 (MQ=255)

             gtGAGTTCCCCAGGGAGATTGGTTCCTGACCCATGCTTACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGACAAG‑GG‑GG‑GATGCaat                                                                               >  2:564978/2‑92 (MQ=255)

               gAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAAcgc                                                                             >  1:257514/1‑93 (MQ=255)

               gAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGAA‑AAAGGG‑GG‑GTTGCAAcgc                                                                             <  2:4578460/93‑1 (MQ=255)

                   tCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGt                                                                         <  1:56230/93‑1 (MQ=255)

                     cccAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTg                                                                       <  1:4191689/93‑1 (MQ=255)

                      ccAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGc                                                                      >  2:1833614/1‑93 (MQ=255)

                      ccAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGc                                                                      >  2:2747624/1‑93 (MQ=255)

                      ccAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGc                                                                      >  1:1717912/1‑93 (MQ=255)

                       cAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCg                                                                     <  1:2767131/93‑1 (MQ=255)

                        aGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGt                                                                    <  2:1892445/93‑1 (MQ=255)

                         ggTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTg                                                                   >  1:4373642/1‑93 (MQ=255)

                         ggTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTg                                                                   >  1:667436/1‑93 (MQ=255)

                         ggTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTg                                                                   >  2:2885321/1‑93 (MQ=255)

                          gTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGc                                                                  >  1:3519307/1‑93 (MQ=255)

                             gATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTcc                                                               >  2:2685973/1‑93 (MQ=255)

                             gATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTcc                                                               >  2:3287720/1‑93 (MQ=255)

                             gATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTcc                                                               >  2:3648389/1‑93 (MQ=255)

                             gATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTcc                                                               >  1:178688/1‑93 (MQ=255)

                             gATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTcc                                                               >  1:1207818/1‑93 (MQ=255)

                               ttGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCt                                                             >  2:793302/1‑93 (MQ=255)

                               ttGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCt                                                             <  2:879063/93‑1 (MQ=255)

                                     ccTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGc                                                       <  2:1562057/93‑1 (MQ=255)

                                      cTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCt                                                      >  1:557454/1‑93 (MQ=255)

                                      cTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCt                                                      <  2:2346296/93‑1 (MQ=255)

                                        gACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACCCCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTTCTTa                                                    <  2:3014994/93‑1 (MQ=255)

                                        gACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTa                                                    <  1:4351068/93‑1 (MQ=255)

                                          cccATGCTAACGCGATTCCGCTAAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGc                                                  <  2:13723/93‑1 (MQ=255)

                                            cATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCgg                                                >  2:3039160/1‑93 (MQ=255)

                                              tGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGaa                                              >  1:2089081/1‑93 (MQ=255)

                                               gCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAag                                             >  2:712407/1‑93 (MQ=255)

                                               gCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAag                                             >  1:2492912/1‑93 (MQ=255)

                                                cTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAaga                                            <  2:4177321/93‑1 (MQ=255)

                                                cTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAaga                                            <  2:311176/93‑1 (MQ=255)

                                                cTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAaga                                            >  1:2441262/1‑93 (MQ=255)

                                                cTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAaga                                            <  2:2504255/93‑1 (MQ=255)

                                                cTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAaga                                            <  2:736869/93‑1 (MQ=255)

                                                 tAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAagag                                           <  1:2681772/93‑1 (MQ=255)

                                                     gcgATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTc                                       <  1:1350225/93‑1 (MQ=255)

                                                      cgATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTcc                                      >  2:4336096/1‑93 (MQ=255)

                                                       gATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTccc                                     >  2:656304/1‑93 (MQ=255)

                                                       gATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTccc                                     <  2:3782404/93‑1 (MQ=255)

                                                       gATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTccc                                     >  1:3934499/1‑93 (MQ=255)

                                                        aTTCCGCTCAAAAATCAGTACAACACGCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCt                                    <  1:1925361/93‑1 (MQ=255)

                                                           ccGCTCAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTc                                 <  1:2886675/93‑1 (MQ=255)

                                                            cGCTCAAAAATCAGTACACCACCCGAGGTA‑AAA‑GGTGG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTca                                <  2:4445295/93‑1 (MQ=255)

                                                            cGCTCAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTca                                >  2:530303/1‑93 (MQ=255)

                                                              cTCAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTcagc                              >  2:1234994/1‑93 (MQ=255)

                                                               tCAAAAATCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTcagca                             <  2:2842714/93‑1 (MQ=255)

                                                                    aaTCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTcc                        <  2:3254799/93‑1 (MQ=255)

                                                                    aaTCAGTAAAACGCCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTcc                        <  1:1643987/93‑1 (MQ=255)

                                                                     aTCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCa                       <  2:2934068/93‑1 (MQ=255)

                                                                     aTCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCa                       <  1:899514/93‑1 (MQ=255)

                                                                      tCAGTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCAt                      <  1:3423495/93‑1 (MQ=255)

                                                                      tCAGTAAAGCACCGGAGGTA‑AAA‑GG‑GGAGATGCAACGCGCGTTGCGTGCTCCCTTTTTTCTTAGCGGAGGAGTTTCCCTTGCAGCAGTTCCAt                      <  2:2038361/93‑1 (MQ=255)

                                                                         gTACAACACCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGcc                   <  1:503675/93‑1 (MQ=255)

                                                                           acaacaCCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTg                 >  1:4523170/1‑93 (MQ=255)

                                                                           acaacaCCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTCTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTg                 <  1:2513762/93‑1 (MQ=255)

                                                                             aacaCCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCg               >  1:814651/1‑93 (MQ=255)

                                                                              acacCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCgg              <  1:160798/93‑1 (MQ=255)

                                                                               cacCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGa             >  2:3345215/1‑93 (MQ=255)

                                                                               cacCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGa             >  2:1726186/1‑93 (MQ=255)

                                                                                acCCGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGaa            >  2:2419180/1‑93 (MQ=255)

                                                                                 cccGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGaaa           >  1:2213089/1‑93 (MQ=255)

                                                                                 cccGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGaaa           >  1:1502632/1‑93 (MQ=255)

                                                                                   cGAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGc         <  1:2419788/93‑1 (MQ=255)

                                                                                   cGAGGGA‑AAAGGG‑GG‑GATGCAACGCGAGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTAAGCAGTTCTATGCCCGCGGTAAga         >  2:3587888/1‑92 (MQ=255)

                                                                                    gAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCa        <  1:4437059/93‑1 (MQ=255)

                                                                                    gAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCa        <  1:1694009/93‑1 (MQ=255)

                                                                                    gAGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCa        >  1:784218/1‑93 (MQ=255)

                                                                                     aGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAg       >  2:4370276/1‑93 (MQ=255)

                                                                                     aGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAg       >  1:154110/1‑93 (MQ=255)

                                                                                     aGGGA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAg       >  1:4381575/1‑93 (MQ=255)

                                                                                      gggA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAGa      <  2:4593212/93‑1 (MQ=255)

                                                                                      gggA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAGa      <  2:2201640/93‑1 (MQ=255)

                                                                                      gggA‑AAAGGG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAGa      <  1:883834/93‑1 (MQ=255)

                                                                                      ggaa‑aag‑GG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAGAt     <  1:2291461/90‑1 (MQ=255)

                                                                                      ggaa‑aag‑GG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAGAt     <  1:1261756/90‑1 (MQ=255)

                                                                                       gaa‑aag‑GG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAGATc    <  2:3714303/91‑1 (MQ=255)

                                                                                       gaa‑aag‑GG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAGATc    <  1:2173553/91‑1 (MQ=255)

                                                                                       gaa‑aag‑GG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAGATc    <  1:2945797/91‑1 (MQ=255)

                                                                                         a‑aag‑GG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAGATCGt  >  2:766824/1‑93 (MQ=255)

GAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGA‑AAA‑GG‑GG‑GATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAGATCGT  >  NC_000913/3874566‑3874747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAA‑GGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAGATCGTTATTGGCT  >  NC_000913/3874571‑3874755

TAATAGCCGTGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAA‑GGG‑GGG                                                                                             <  SRR4302535.1084400/94‑1 (MQ=60)

 AATAGCCGTGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAA‑GGG‑GGga                                                                                            <  SRR4302535.40129/94‑3 (MQ=60)

  ATAGCCGTGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAA‑GGG‑GGgag                                                                                           >  SRR4302535.4142181/1‑91 (MQ=60)

   TAGCCGTGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATG                                                                                           >  SRR4302535.1121846/1‑94 (MQ=60)

   TAGCCGTGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATG                                                                                           <  SRR4302535.4522845/94‑1 (MQ=60)

    AGCCGTGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGC                                                                                          >  SRR4302535.1115218/1‑94 (MQ=60)

    AGCCGTGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGC                                                                                          >  SRR4302535.4338287/1‑94 (MQ=60)

    AGCCGTGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGC                                                                                          >  SRR4302535.2323870/1‑94 (MQ=60)

      CCGTGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAA                                                                                        >  SRR4302535.2838559/1‑94 (MQ=60)

       CGTGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAAC                                                                                       >  SRR4302535.4043330/1‑94 (MQ=60)

       CGTGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAAC                                                                                       >  SRR4302535.3610333/1‑94 (MQ=60)

        GTGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACG                                                                                      <  SRR4302535.252359/94‑1 (MQ=60)

        GTGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACG                                                                                      >  SRR4302535.4674596/1‑94 (MQ=60)

         TGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGC                                                                                     >  SRR4302535.259291/1‑94 (MQ=60)

           AGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGC                                                                                   >  SRR4302535.454590/1‑94 (MQ=60)

              TCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTT                                                                                <  SRR4302535.56637/94‑1 (MQ=60)

               CCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTG                                                                               <  SRR4302535.3998076/94‑1 (MQ=60)

               CCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTAAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGGTGCAACGCGCGGTG                                                                               >  SRR4302535.1472404/1‑94 (MQ=60)

                CCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGC                                                                              <  SRR4302535.4220374/94‑1 (MQ=60)

                CCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGC                                                                              >  SRR4302535.885294/1‑94 (MQ=60)

                CCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGC                                                                              >  SRR4302535.1730342/1‑94 (MQ=60)

                 CCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCG                                                                             <  SRR4302535.3589534/94‑1 (MQ=60)

                  CAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGT                                                                            <  SRR4302535.2786836/94‑1 (MQ=60)

                  CAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGT                                                                            >  SRR4302535.4153834/1‑94 (MQ=60)

                   AGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTG                                                                           >  SRR4302535.672089/1‑94 (MQ=60)

                   AGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTG                                                                           <  SRR4302535.227581/94‑1 (MQ=60)

                   AGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTG                                                                           >  SRR4302535.4403563/1‑94 (MQ=60)

                    GGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGC                                                                          <  SRR4302535.3949691/94‑1 (MQ=60)

                    GGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGC                                                                          >  SRR4302535.3543755/1‑94 (MQ=60)

                     GTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCT                                                                         >  SRR4302535.1667101/1‑94 (MQ=60)

                     GTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCT                                                                         <  SRR4302535.886982/94‑1 (MQ=60)

                     GTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCT                                                                         >  SRR4302535.2909761/1‑94 (MQ=60)

                       AGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCC                                                                       >  SRR4302535.1216499/1‑94 (MQ=60)

                       AGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCC                                                                       >  SRR4302535.179928/1‑94 (MQ=60)

                        GATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCC                                                                      <  SRR4302535.3344299/94‑1 (MQ=60)

                          TTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTT                                                                    <  SRR4302535.2184912/94‑1 (MQ=60)

                           TGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTT                                                                   <  SRR4302535.2345361/94‑1 (MQ=60)

                             GTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTT                                                                 <  SRR4302535.1577618/94‑1 (MQ=60)

                              TTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTG                                                                >  SRR4302535.1535097/1‑94 (MQ=60)

                                CCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCT                                                              >  SRR4302535.561358/1‑94 (MQ=60)

                                   GACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAG                                                           <  SRR4302535.2822208/94‑1 (MQ=60)

                                   GACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAG                                                           <  SRR4302535.4380837/94‑1 (MQ=60)

                                   GACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAG                                                           <  SRR4302535.798516/94‑1 (MQ=60)

                                    ACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCTCTTTTTGCTTAGC                                                          >  SRR4302535.2247235/1‑94 (MQ=60)

                                    ACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGC                                                          <  SRR4302535.3563881/94‑1 (MQ=60)

                                     CCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCG                                                         <  SRR4302535.1784839/94‑1 (MQ=60)

                                      CCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGG                                                        <  SRR4302535.3966477/94‑1 (MQ=60)

                                      CCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGG                                                        >  SRR4302535.562742/1‑94 (MQ=60)

                                      CCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGG                                                        >  SRR4302535.2474532/1‑94 (MQ=60)

                                        ATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAA                                                      >  SRR4302535.2104134/1‑94 (MQ=60)

                                        ATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAA                                                      >  SRR4302535.168659/1‑94 (MQ=60)

                                        ATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAA                                                      >  SRR4302535.787600/1‑94 (MQ=60)

                                         TGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAG                                                     >  SRR4302535.2510901/1‑94 (MQ=60)

                                          GCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGA                                                    >  SRR4302535.4281888/1‑94 (MQ=60)

                                          GCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGA                                                    >  SRR4302535.2458849/1‑94 (MQ=60)

                                           CTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAG                                                   >  SRR4302535.4648992/1‑94 (MQ=60)

                                            TAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGT                                                  <  SRR4302535.1889522/94‑1 (MQ=60)

                                            TAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGT                                                  <  SRR4302535.2700956/94‑1 (MQ=60)

                                               CGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTC                                               >  SRR4302535.1104144/1‑94 (MQ=60)

                                                GCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCC                                              <  SRR4302535.1359868/94‑1 (MQ=60)

                                                GCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCC                                              <  SRR4302535.1160735/94‑1 (MQ=60)

                                                 CGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCC                                             >  SRR4302535.3961346/1‑94 (MQ=60)

                                                 CGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCC                                             >  SRR4302535.4522337/1‑94 (MQ=60)

                                                  GATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCT                                            <  SRR4302535.2686830/94‑1 (MQ=60)

                                                   ATTCCGCTCAAAAATCAGTACAACACGCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTT                                           <  SRR4302535.1939241/94‑1 (MQ=60)

                                                   ATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTT                                           >  SRR4302535.4400416/1‑94 (MQ=60)

                                                    TTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTT                                          >  SRR4302535.649893/1‑94 (MQ=60)

                                                     TCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTC                                         <  SRR4302535.286962/94‑1 (MQ=60)

                                                     TCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTC                                         <  SRR4302535.1236486/94‑1 (MQ=60)

                                                      CCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCA                                        <  SRR4302535.2907098/94‑1 (MQ=60)

                                                      CCGCTCAAAAATCAGTACAACACCCGAGGGAAAA‑GGGTGGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCA                                        <  SRR4302535.1207068/94‑1 (MQ=60)

                                                       CGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGACGAGCTTCCCTTTCAG                                       >  SRR4302535.801832/1‑94 (MQ=60)

                                                       CGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAG                                       >  SRR4302535.1259466/1‑94 (MQ=60)

                                                       CGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAG                                       <  SRR4302535.3708015/94‑1 (MQ=60)

                                                        GCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGC                                      >  SRR4302535.510054/1‑94 (MQ=60)

                                                           CAAAAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGT                                   >  SRR4302535.4410635/1‑94 (MQ=60)

                                                              AAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCC                                <  SRR4302535.4409610/94‑1 (MQ=60)

                                                              AAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCC                                >  SRR4302535.3521238/1‑94 (MQ=60)

                                                              AAATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCC                                <  SRR4302535.1405583/94‑1 (MQ=60)

                                                               AATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCA                               >  SRR4302535.3143394/1‑94 (MQ=60)

                                                               AATCAGTAAAACGCCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCA                               <  SRR4302535.1655871/94‑1 (MQ=60)

                                                                ATCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCAT                              <  SRR4302535.905883/94‑1 (MQ=60)

                                                                ATCAGTACAACACCCAAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTGCAGCAGTTCCAT                              >  SRR4302535.1739120/1‑94 (MQ=60)

                                                                 TCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATG                             <  SRR4302535.1990463/94‑1 (MQ=60)

                                                                 TCAGTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATG                             <  SRR4302535.3447303/94‑1 (MQ=60)

                                                                    GTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCT                          <  SRR4302535.507196/94‑1 (MQ=60)

                                                                    GTACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCGTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCT                          <  SRR4302535.2539805/94‑1 (MQ=60)

                                                                     TACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTG                         >  SRR4302535.4554160/1‑94 (MQ=60)

                                                                     TACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTG                         <  SRR4302535.4322869/94‑1 (MQ=60)

                                                                      ACAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTCTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGC                        <  SRR4302535.2531886/94‑1 (MQ=60)

                                                                       CAACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCG                       >  SRR4302535.820363/1‑94 (MQ=60)

                                                                        AACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGG                      >  SRR4302535.1872512/1‑94 (MQ=60)

                                                                        AACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGG                      >  SRR4302535.2771504/1‑94 (MQ=60)

                                                                         ACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGA                     <  SRR4302535.2769174/94‑1 (MQ=60)

                                                                         ACACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGA                     <  SRR4302535.161925/94‑1 (MQ=60)

                                                                          CACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAA                    >  SRR4302535.954173/1‑94 (MQ=60)

                                                                          CACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGCGCTCTCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAA                    >  SRR4302535.1458272/1‑94 (MQ=60)

                                                                           ACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAA                   >  SRR4302535.2228848/1‑94 (MQ=60)

                                                                           ACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAA                   >  SRR4302535.1513442/1‑94 (MQ=60)

                                                                           ACCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAA                   >  SRR4302535.4497866/1‑94 (MQ=60)

                                                                            CCCGAGGGAAAAGGGG‑GGATGCTACGCGCGTTGCGTGCTCCCTATTTGCTTAGCGGAAGAGTTTCGCTTTCAGCAGTTCCATGCCTGCGGctag                  >  SRR4302535.364711/1‑90 (MQ=60)

                                                                            CCCGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAG                  >  SRR4302535.2954476/1‑94 (MQ=60)

                                                                              CGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCA                >  SRR4302535.789719/1‑94 (MQ=60)

                                                                              CGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCA                >  SRR4302535.599619/1‑94 (MQ=60)

                                                                              CGAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCA                <  SRR4302535.2437212/94‑1 (MQ=60)

                                                                               GAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAG               >  SRR4302535.155191/1‑94 (MQ=60)

                                                                               GAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAG               <  SRR4302535.4467467/94‑1 (MQ=60)

                                                                               GAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAG               >  SRR4302535.4411551/1‑94 (MQ=60)

                                                                               GAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAG               <  SRR4302535.1706260/94‑1 (MQ=60)

                                                                               GAGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAG               >  SRR4302535.2111651/1‑94 (MQ=60)

                                                                                GGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAGA              <  SRR4302535.2419097/94‑1 (MQ=60)

                                                                                AGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAGA              >  SRR4302535.4413006/1‑94 (MQ=60)

                                                                                AGGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAGA              >  SRR4302535.1395756/1‑94 (MQ=60)

                                                                                 GGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAGAT             <  SRR4302535.2795994/94‑1 (MQ=60)

                                                                                 GGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAGAT             <  SRR4302535.1710235/94‑1 (MQ=60)

                                                                                 GGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAGAT             <  SRR4302535.897555/94‑1 (MQ=60)

                                                                                 GGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAGAT             <  SRR4302535.890089/94‑1 (MQ=60)

                                                                                 GGGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAGAT             <  SRR4302535.2227443/94‑1 (MQ=60)

                                                                                  GGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAGATC            <  SRR4302535.1617903/94‑1 (MQ=60)

                                                                                  GGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAGATC            <  SRR4302535.2307854/94‑1 (MQ=60)

                                                                                  GGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAGATC            <  SRR4302535.1270784/94‑1 (MQ=60)

                                                                                  GGAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAGATC            <  SRR4302535.1311382/94‑1 (MQ=60)

                                                                                   GAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAGATCG           <  SRR4302535.24821/94‑1 (MQ=60)

                                                                                   GAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAGATCG           <  SRR4302535.2966499/94‑1 (MQ=60)

                                                                                   GAAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAGATCG           <  SRR4302535.2189101/94‑1 (MQ=60)

                                                                                    AAAAGGGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAGATCGT          >  SRR4302535.3377192/1‑94 (MQ=60)

                                                                                      AG‑GGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAGATCGTTAT       >  SRR4302535.2212693/1‑94 (MQ=60)

                                                                                      AG‑GGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAGATCGTTAT       >  SRR4302535.3830685/1‑94 (MQ=60)

                                                                                       G‑GGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAGATCGTTATT      >  SRR4302535.3903018/1‑94 (MQ=60)

                                                                                       G‑GGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAGATCGTTATT      <  SRR4302535.4369332/94‑1 (MQ=60)

                                                                                         GGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAGATCGTTATTG     <  SRR4302535.2636640/94‑1 (MQ=60)

                                                                                         GGG‑GGATGCAACGCGCGTTGCGTCCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAGATCGTTATTG     <  SRR4302535.4147627/94‑1 (MQ=60)

                                                                                          GG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAGATCGTTATTGG    <  SRR4302535.1817522/94‑1 (MQ=60)

                                                                                          GG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAGATCGTTATTGG    <  SRR4302535.2058073/94‑1 (MQ=60)

                                                                                           G‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAGATCGTTATTGGC   <  SRR4302535.1451617/94‑1 (MQ=60)

                                                                                           G‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAGATCGTTATTGGC   >  SRR4302535.810624/1‑94 (MQ=60)

                                                                                             GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAGATCGTTATTGGCT  >  SRR4302535.497105/1‑94 (MQ=60)

                                                                                             GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAGATCGTTATTGGCT  <  SRR4302535.3674553/94‑1 (MQ=60)

                                                                                             GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAGATCGTTATTGGCT  <  SRR4302535.4137405/94‑1 (MQ=60)

TAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAA‑GGG‑GGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCCCTTTCAGCAGTTCCATGCCTGCGGAAAGCAGATCGTTATTGGCT  >  NC_000913/3874571‑3874755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`