Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,892,388	A→G	E36E (GAA→GAG)	yieF →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,892,388	0	A	G	96.6%	787.8 / 10.9	264	E36E (GAA→GAG)	yieF	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (4/5); new base G (124/131); total (128/136)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.47e-01

Only 100 of 272 total aligned reads displayed.

AGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCT  >  NC_000913/3892297‑3892477

 ggtggtTACGTTACTGGGGAGCCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGt                                                                                         >  1:2530535/1‑93 (MQ=255)

         cgttacTGGGGAGCCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGt                                                                                 <  2:1723126/93‑1 (MQ=255)

          gttacTGGGGAGCCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGtt                                                                                <  2:738353/93‑1 (MQ=255)

          gttacTGGGGAGCCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGtt                                                                                <  1:3268472/93‑1 (MQ=255)

          gttacTGGGGAGCCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGtt                                                                                >  1:717549/1‑93 (MQ=255)

           ttacTGGGGAGCCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTa                                                                               >  2:4444203/1‑93 (MQ=255)

           ttacTGGGGAGCCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTa                                                                               >  2:222212/1‑93 (MQ=255)

             acTGGGGAGCCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAACATTGCTCCGGCGAGCATGGAGGTCAATGCGTTAcc                                                                             >  1:2799067/1‑93 (MQ=255)

               tGGGGAGCCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTAccat                                                                           >  1:4480259/1‑93 (MQ=255)

               tGGGGAGCCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTAccat                                                                           <  1:745446/93‑1 (MQ=255)

                ggggAGCCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTAccatc                                                                          >  2:152746/1‑93 (MQ=255)

                 gggAGCCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTATCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTAccatcc                                                                         <  2:4621040/93‑1 (MQ=255)

                 gggAGCCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTAccatcc                                                                         >  2:3778547/1‑93 (MQ=255)

                    aGCCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCAtt                                                                      <  2:5490/93‑1 (MQ=255)

                    aGCCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCAtt                                                                      <  1:3227641/93‑1 (MQ=255)

                    aGCCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCAtt                                                                      <  2:3873679/93‑1 (MQ=255)

                     gCCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTg                                                                     >  2:3522982/1‑93 (MQ=255)

                       cTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGcc                                                                   >  2:1568420/1‑93 (MQ=255)

                       cTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGcc                                                                   >  1:1804967/1‑93 (MQ=255)

                       cTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGcc                                                                   >  1:3054939/1‑93 (MQ=255)

                        tGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCg                                                                  >  1:2056854/1‑93 (MQ=255)

                         gcgcAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGa                                                                 >  2:3289272/1‑93 (MQ=255)

                           gcAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACa                                                               <  1:2099142/93‑1 (MQ=255)

                           gcAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACa                                                               >  1:4051561/1‑93 (MQ=255)

                           gcAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACa                                                               >  1:4632181/1‑93 (MQ=255)

                             aaaGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACAtt                                                             >  1:4035077/1‑93 (MQ=255)

                                 gCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCt                                                         <  1:3789809/93‑1 (MQ=255)

                                 gCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCt                                                         >  1:1600620/1‑93 (MQ=255)

                                  cTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCtt                                                        >  1:3007653/1‑93 (MQ=255)

                                     aTTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTa                                                     <  2:133752/93‑1 (MQ=255)

                                     aTTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTa                                                     <  2:3592252/93‑1 (MQ=255)

                                     aTTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTa                                                     <  1:30806/93‑1 (MQ=255)

                                      tttAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTAt                                                    >  1:3981690/1‑93 (MQ=255)

                                      tttAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTAt                                                    >  1:1728994/1‑93 (MQ=255)

                                      tttAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTAt                                                    >  1:996160/1‑93 (MQ=255)

                                       ttAATGGCATTGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATg                                                   <  1:2300708/93‑1 (MQ=255)

                                         aaTGGCATGGTTGCACGTCCCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGAc                                                 <  2:2353232/93‑1 (MQ=255)

                                         aaTGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGAc                                                 <  1:242323/93‑1 (MQ=255)

                                         aaTGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGAc                                                 >  1:1440760/1‑93 (MQ=255)

                                          aTGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACg                                                >  2:2255764/1‑93 (MQ=255)

                                           tGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGc                                               <  2:240279/93‑1 (MQ=255)

                                           tGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGc                                               <  1:3573287/93‑1 (MQ=255)

                                            ggCATGGTTGCACGTCCCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCt                                              <  1:51559/93‑1 (MQ=255)

                                             gCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTg                                             >  1:3490925/1‑93 (MQ=255)

                                             gCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTg                                             >  2:405137/1‑93 (MQ=255)

                                              cATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGa                                            >  2:2660957/1‑93 (MQ=255)

                                              cATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGa                                            >  1:3162420/1‑93 (MQ=255)

                                               aTGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGAc                                           <  2:4384275/93‑1 (MQ=255)

                                               aTGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGAc                                           <  2:616481/93‑1 (MQ=255)

                                               aTGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGAc                                           <  2:2300507/93‑1 (MQ=255)

                                               aTGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGAc                                           >  1:234040/1‑93 (MQ=255)

                                                tGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACg                                          >  2:2140893/1‑93 (MQ=255)

                                                  gTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTa                                        <  2:2961234/93‑1 (MQ=255)

                                                   ttGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTAc                                       <  1:4522747/93‑1 (MQ=255)

                                                    tGCACGTCCCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTAca                                      <  1:837020/93‑1 (MQ=255)

                                                    tGCACGTACCCTGCTGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTAca                                      <  2:4218309/93‑1 (MQ=255)

                                                    tGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTAca                                      <  2:2516090/93‑1 (MQ=255)

                                                    tGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTAca                                      <  1:4409218/93‑1 (MQ=255)

                                                    tGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTAca                                      <  1:333419/93‑1 (MQ=255)

                                                      cACGTACCCTGCCGAAAATTGCTCCCGCGAGCATGGAGGGCAATGCGTTACCATCGATTGCCGAGCTGCCCTTGTATGACGCTGGAGTAcagc                                    >  2:2050478/1‑93 (MQ=255)

                                                         gTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAgg                                 >  2:3441225/1‑93 (MQ=255)

                                                         gTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAgg                                 >  2:3653742/1‑93 (MQ=255)

                                                         gTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAgg                                 >  2:3151296/1‑93 (MQ=255)

                                                           aCCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGgaa                               <  2:3568201/93‑1 (MQ=255)

                                                             ccTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACATCAGgaaga                             >  2:304415/1‑93 (MQ=255)

                                                              cTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGgaagaa                            >  1:1001491/1‑93 (MQ=255)

                                                              cTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGgaagaa                            >  2:1081737/1‑93 (MQ=255)

                                                               tGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGaagaag                           >  1:153175/1‑93 (MQ=255)

                                                               tGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGaagaag                           >  2:2110560/1‑93 (MQ=255)

                                                               tGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGaagaag                           <  2:262438/93‑1 (MQ=255)

                                                               tGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGaagaag                           <  2:2874425/93‑1 (MQ=255)

                                                               tGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGaagaag                           >  2:104017/1‑93 (MQ=255)

                                                                gCCGAAAATTGCTCCGCCGAGCATGGATGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTCACGTACAGCAGGAAGAAgg                          <  2:4584796/93‑1 (MQ=255)

                                                                 ccGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGt                         <  2:1457047/93‑1 (MQ=255)

                                                                 ccGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGt                         <  2:2012106/93‑1 (MQ=255)

                                                                  cGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCGGGAAGAAGGtt                        <  1:1300385/93‑1 (MQ=255)

                                                                  cGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGtt                        <  1:4157113/93‑1 (MQ=255)

                                                                  cGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGtt                        <  2:1372632/93‑1 (MQ=255)

                                                                   gAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGttt                       >  1:1044484/1‑93 (MQ=255)

                                                                    aaaaTTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGtttt                      >  1:1163602/1‑93 (MQ=255)

                                                                     aaaTTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTc                     <  2:3334379/93‑1 (MQ=255)

                                                                     aaaTTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTc                     <  2:1751130/93‑1 (MQ=255)

                                                                       aTTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCa                   <  1:4603668/93‑1 (MQ=255)

                                                                       aTTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCa                   <  1:2962396/93‑1 (MQ=255)

                                                                           cTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCaa               >  2:1690297/1‑93 (MQ=255)

                                                                           cTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCaa               <  2:2434938/93‑1 (MQ=255)

                                                                            tCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAAc              <  2:514736/93‑1 (MQ=255)

                                                                              cGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACgg            <  2:4307083/93‑1 (MQ=255)

                                                                              cGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACgg            <  2:1134073/93‑1 (MQ=255)

                                                                               ggCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGt           <  1:2281736/93‑1 (MQ=255)

                                                                                gCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGtt          >  2:3787154/1‑93 (MQ=255)

                                                                                  gAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGa        <  1:4424386/93‑1 (MQ=255)

                                                                                  gAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGa        >  2:1624826/1‑93 (MQ=255)

                                                                                  gAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGa        >  1:2700504/1‑93 (MQ=255)

                                                                                    gCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAg      <  1:1378332/93‑1 (MQ=255)

                                                                                       tGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGctc   >  1:3699842/1‑93 (MQ=255)

                                                                                       tGGAAGTCAATGAGATACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAACTTTTTCCAGCAACGGTTGAAGctc   <  2:1902872/93‑1 (MQ=255)

                                                                                        ggAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGctct  >  1:658156/1‑93 (MQ=255)

                                                                                        ggAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGctct  <  2:4421081/93‑1 (MQ=255)

                                                                                        ggAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGctct  <  2:286481/93‑1 (MQ=255)

AGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCT  >  NC_000913/3892297‑3892477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCG  >  NC_000913/3892295‑3892481

GCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAG                                                                                               <  SRR4302535.3976042/94‑1 (MQ=60)

  AGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGT                                                                                             >  SRR4302535.2548770/1‑94 (MQ=60)

  AGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGT                                                                                             <  SRR4302535.3985138/94‑1 (MQ=60)

   GGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTC                                                                                            >  SRR4302535.2847749/1‑94 (MQ=60)

    GTGGTTACATTACTGGGGAGCCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCA                                                                                           <  SRR4302535.2995904/94‑1 (MQ=60)

       GTTACGTTACTGGGGAGCCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATG                                                                                        >  SRR4302535.489835/1‑94 (MQ=60)

        TTACGTTACTGGGGAGCCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGC                                                                                       >  SRR4302535.1617751/1‑94 (MQ=60)

           CGTTACTGGGGAGCCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTT                                                                                    >  SRR4302535.722589/1‑94 (MQ=60)

           CGTTACTGGGGAGCCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTT                                                                                    >  SRR4302535.4474371/1‑94 (MQ=60)

            GTTACTGGGGAGCCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTA                                                                                   <  SRR4302535.3291178/94‑1 (MQ=60)

             TTACTGGGGAGCCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTAC                                                                                  >  SRR4302535.1948119/1‑94 (MQ=60)

              TACTGGGGAGCCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAACATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACC                                                                                 >  SRR4302535.2818984/1‑94 (MQ=60)

              TACTGGGGAGCCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACC                                                                                 <  SRR4302535.3749450/94‑1 (MQ=60)

               ACTGGGGAGCCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCA                                                                                >  SRR4302535.4527519/1‑94 (MQ=60)

               ACTGGGGAGCCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCA                                                                                <  SRR4302535.63612/94‑1 (MQ=60)

                ccggggacgCTGCGCAAAGGCTCATTTAATGGCATGCTTGCACGTGCCCCGCCGAAAATTGATCCGGCGAGCATGGAGGTCAATGCGTTCCCAT                                                                               <  SRR4302535.652532/85‑1 (MQ=60)

                CTGGGGAGCCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCAT                                                                               >  SRR4302535.4511014/1‑94 (MQ=60)

                 TGGGGAGCCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATC                                                                              <  SRR4302535.750685/94‑1 (MQ=60)

                 TGGGGAGCCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATC                                                                              >  SRR4302535.1443100/1‑94 (MQ=60)

                      AGCCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTG                                                                         >  SRR4302535.2345624/1‑94 (MQ=60)

                      AGCCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTG                                                                         <  SRR4302535.1223231/94‑1 (MQ=60)

                      AGCCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTG                                                                         <  SRR4302535.3250010/94‑1 (MQ=60)

                       GCCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGC                                                                        >  SRR4302535.265546/1‑94 (MQ=60)

                        CCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCC                                                                       >  SRR4302535.1818011/1‑94 (MQ=60)

                        CCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCC                                                                       >  SRR4302535.3076322/1‑94 (MQ=60)

                         CTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCG                                                                      >  SRR4302535.1493225/1‑94 (MQ=60)

                         CTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCG                                                                      >  SRR4302535.2071673/1‑94 (MQ=60)

                            CGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACA                                                                   >  SRR4302535.4079298/1‑94 (MQ=60)

                            CGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACA                                                                   >  SRR4302535.4663610/1‑94 (MQ=60)

                             GCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACAT                                                                  >  SRR4302535.3000818/1‑94 (MQ=60)

                             GCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACAT                                                                  >  SRR4302535.3276998/1‑94 (MQ=60)

                             GCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACAT                                                                  <  SRR4302535.2114283/94‑1 (MQ=60)

                              CAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATT                                                                 >  SRR4302535.4062702/1‑94 (MQ=60)

                                AAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCC                                                               >  SRR4302535.4009230/1‑94 (MQ=60)

                                  GGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCT                                                             >  SRR4302535.1612167/1‑94 (MQ=60)

                                  GGCTAATTTAATGGCATGGTTGCACGTCCCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCT                                                             <  SRR4302535.4652407/94‑1 (MQ=60)

                                   GCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTT                                                            <  SRR4302535.3815945/94‑1 (MQ=60)

                                   GCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTT                                                            >  SRR4302535.3028720/1‑94 (MQ=60)

                                   GCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTT                                                            >  SRR4302535.3596820/1‑94 (MQ=60)

                                   GCTCATTTAATGGCATGGTTGCACGAACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTT                                                            <  SRR4302535.4560759/94‑1 (MQ=60)

                                    CTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTG                                                           <  SRR4302535.4704706/94‑1 (MQ=60)

                                     TCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGT                                                          <  SRR4302535.790334/94‑1 (MQ=60)

                                      CATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTA                                                         >  SRR4302535.4065717/1‑94 (MQ=60)

                                       ATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTAT                                                        <  SRR4302535.31045/94‑1 (MQ=60)

                                       ATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTAT                                                        >  SRR4302535.4008894/1‑94 (MQ=60)

                                       ATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTAT                                                        >  SRR4302535.1003235/1‑94 (MQ=60)

                                       ATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTAT                                                        >  SRR4302535.1330422/1‑94 (MQ=60)

                                       ATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTAT                                                        >  SRR4302535.1440713/1‑94 (MQ=60)

                                       ATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTAT                                                        >  SRR4302535.1741489/1‑94 (MQ=60)

                                       ATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTAT                                                        <  SRR4302535.2158064/94‑1 (MQ=60)

                                        TTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATG                                                       <  SRR4302535.4485016/94‑1 (MQ=60)

                                         TTAATGGCATTGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGA                                                      <  SRR4302535.2317173/94‑1 (MQ=60)

                                          TAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGAC                                                     >  SRR4302535.2353698/1‑94 (MQ=60)

                                          TAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGAC                                                     >  SRR4302535.1451066/1‑94 (MQ=60)

                                          TAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGAC                                                     <  SRR4302535.3125112/94‑1 (MQ=60)

                                           AATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACG                                                    <  SRR4302535.243985/94‑1 (MQ=60)

                                           AATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACG                                                    <  SRR4302535.3292474/94‑1 (MQ=60)

                                           AATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACG                                                    >  SRR4302535.3215416/1‑94 (MQ=60)

                                           AATGGCATGGTTGCACGTACCCCGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTCCCATCCATTGCCGACATTCCCTTGTATGACG                                                    <  SRR4302535.3953027/94‑1 (MQ=60)

                                            ATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGC                                                   <  SRR4302535.2591504/94‑1 (MQ=60)

                                             TGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCT                                                  <  SRR4302535.3598064/94‑1 (MQ=60)

                                              GGCATGGTTGCACGTCCCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTG                                                 <  SRR4302535.51937/94‑1 (MQ=60)

                                              GGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTG                                                 >  SRR4302535.3515186/1‑94 (MQ=60)

                                               GCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGA                                                >  SRR4302535.3184354/1‑94 (MQ=60)

                                                CATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGAC                                               <  SRR4302535.1472183/94‑1 (MQ=60)

                                                CATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGAC                                               >  SRR4302535.4030493/1‑94 (MQ=60)

                                                CATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGAC                                               >  SRR4302535.235655/1‑94 (MQ=60)

                                                CATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGAC                                               >  SRR4302535.158437/1‑94 (MQ=60)

                                                CATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGAC                                               >  SRR4302535.2790051/1‑94 (MQ=60)

                                                CATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGAC                                               <  SRR4302535.3078010/94‑1 (MQ=60)

                                                 ATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACG                                              <  SRR4302535.745635/94‑1 (MQ=60)

                                                 ATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACG                                              <  SRR4302535.743827/94‑1 (MQ=60)

                                                     TTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACA                                          <  SRR4302535.4553737/94‑1 (MQ=60)

                                                      TGGACCTACCCTGCCGAAAATTGCCCCCGAGGGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAG                                         <  SRR4302535.1091625/94‑1 (MQ=60)

                                                      TGCACGTCCCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAG                                         <  SRR4302535.842895/94‑1 (MQ=60)

                                                      TGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAG                                         <  SRR4302535.335726/94‑1 (MQ=60)

                                                      TGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAG                                         <  SRR4302535.4439390/94‑1 (MQ=60)

                                                      TGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAG                                         <  SRR4302535.70211/94‑1 (MQ=60)

                                                       GCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGC                                        <  SRR4302535.4335229/94‑1 (MQ=60)

                                                         ACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAG                                      <  SRR4302535.546130/94‑1 (MQ=60)

                                                         ACGTACCCTGCAGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAG                                      <  SRR4302535.2120230/94‑1 (MQ=60)

                                                          CTTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGG                                     <  SRR4302535.2848882/94‑1 (MQ=60)

                                                          CGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGG                                     >  SRR4302535.3195470/1‑94 (MQ=60)

                                                          CGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGG                                     <  SRR4302535.2302766/94‑1 (MQ=60)

                                                          CGTACCCTGCCCAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGG                                     <  SRR4302535.1754957/94‑1 (MQ=60)

                                                           GTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGAGGTACAGCAGGA                                    >  SRR4302535.3179441/1‑94 (MQ=60)

                                                               CCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAA                                >  SRR4302535.4099094/1‑94 (MQ=60)

                                                               CCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAA                                >  SRR4302535.1008607/1‑94 (MQ=60)

                                                               CCTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAA                                >  SRR4302535.2314102/1‑94 (MQ=60)

                                                                CTGCCGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAG                               >  SRR4302535.154250/1‑94 (MQ=60)

                                                                  GCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGT                             <  SRR4302535.1128010/94‑1 (MQ=60)

                                                                    CGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCGGGAAGAAGGTTT                           <  SRR4302535.1309654/94‑1 (MQ=60)

                                                                    CGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTT                           >  SRR4302535.1584838/1‑94 (MQ=60)

                                                                    CGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTT                           >  SRR4302535.3584441/1‑94 (MQ=60)

                                                                    CGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTT                           <  SRR4302535.4185569/94‑1 (MQ=60)

                                                                    CGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTT                           >  SRR4302535.1051887/1‑94 (MQ=60)

                                                                    CGAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTT                           >  SRR4302535.3047012/1‑94 (MQ=60)

                                                                     GAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTT                          >  SRR4302535.1171913/1‑94 (MQ=60)

                                                                     GAAAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTT                          <  SRR4302535.4083215/94‑1 (MQ=60)

                                                                       AAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCC                        <  SRR4302535.2523389/94‑1 (MQ=60)

                                                                       AAATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCC                        <  SRR4302535.3839647/94‑1 (MQ=60)

                                                                        AATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCA                       >  SRR4302535.2429992/1‑94 (MQ=60)

                                                                        AATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCA                       <  SRR4302535.347864/94‑1 (MQ=60)

                                                                        AATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCA                       <  SRR4302535.873935/94‑1 (MQ=60)

                                                                         ATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAG                      >  SRR4302535.4235672/1‑94 (MQ=60)

                                                                         ATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAG                      <  SRR4302535.2983191/94‑1 (MQ=60)

                                                                         ATTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAG                      <  SRR4302535.4634968/94‑1 (MQ=60)

                                                                          TTGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGC                     <  SRR4302535.1045374/94‑1 (MQ=60)

                                                                           TGCTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCA                    <  SRR4302535.3626811/94‑1 (MQ=60)

                                                                             CTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAAC                  >  SRR4302535.1808637/1‑94 (MQ=60)

                                                                             CTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAAC                  <  SRR4302535.4576768/94‑1 (MQ=60)

                                                                             CTCCGGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAAC                  <  SRR4302535.877965/94‑1 (MQ=60)

                                                                                CGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGT               <  SRR4302535.1224013/94‑1 (MQ=60)

                                                                                 GGCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTT              <  SRR4302535.2298036/94‑1 (MQ=60)

                                                                                  GCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTG             >  SRR4302535.4539177/1‑94 (MQ=60)

                                                                                  GCGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTG             >  SRR4302535.2100433/1‑94 (MQ=60)

                                                                                   CGAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGA            >  SRR4302535.2719800/1‑94 (MQ=60)

                                                                                    GAGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAA           <  SRR4302535.4454688/94‑1 (MQ=60)

                                                                                     AGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAG          <  SRR4302535.3756906/94‑1 (MQ=60)

                                                                                     AGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAG          >  SRR4302535.1067349/1‑94 (MQ=60)

                                                                                     AGCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAG          <  SRR4302535.3287605/94‑1 (MQ=60)

                                                                                      GCATGGAGGTCAATGCGTTACCATCCATTGCCGGCATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGC         >  SRR4302535.1941551/1‑94 (MQ=60)

                                                                                      GCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGC         <  SRR4302535.4442161/94‑1 (MQ=60)

                                                                                      GCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGC         >  SRR4302535.3415779/1‑94 (MQ=60)

                                                                                      GCATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGC         <  SRR4302535.1388166/94‑1 (MQ=60)

                                                                                      GCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGC         >  SRR4302535.3069270/1‑94 (MQ=60)

                                                                                        ATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTC       >  SRR4302535.3725470/1‑94 (MQ=60)

                                                                                        ATGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTC       >  SRR4302535.814350/1‑94 (MQ=60)

                                                                                         TGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCT      <  SRR4302535.2545226/94‑1 (MQ=60)

                                                                                         TGGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCT      >  SRR4302535.662751/1‑94 (MQ=60)

                                                                                          GGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGGTCTG     >  SRR4302535.2757805/1‑94 (MQ=60)

                                                                                          GGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTG     >  SRR4302535.755963/1‑94 (MQ=60)

                                                                                          GGAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTG     >  SRR4302535.4632258/1‑94 (MQ=60)

                                                                                           GAGGTCAATGCGTTCCCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGG    <  SRR4302535.3098686/94‑1 (MQ=60)

                                                                                           GAGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGG    <  SRR4302535.2112419/94‑1 (MQ=60)

                                                                                            AGGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGC   >  SRR4302535.3793108/1‑94 (MQ=60)

                                                                                             GGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCG  <  SRR4302535.1812584/94‑1 (MQ=60)

                                                                                             GGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACCCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCG  >  SRR4302535.3325576/1‑94 (MQ=60)

GCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCG  >  NC_000913/3892295‑3892481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 5 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`