Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,901,321	T→G	A3A (GCA→GCC)	bglB ←	phospho‑beta‑glucosidase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,901,321	0	T	G	99.6%	787.4 / NA	248	A3A (GCA→GCC)	bglB	phospho‑beta‑glucosidase B
Reads supporting (aligned to +/- strand): ref base T (1/0); major base G (129/117); minor base A (1/0); total (131/117)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 251 total aligned reads displayed.

TGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAATAC  >  NC_000913/3901231‑3901410

tGAGTTCGAGATCCCTTCGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGc                                                                                         <  2:3075750/93‑1 (MQ=255)

  aGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGGTTCTGGAAAGGCtt                                                                                       >  1:3828800/1‑93 (MQ=255)

    gTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTc                                                                                     <  1:4101799/93‑1 (MQ=255)

     tCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCa                                                                                    <  1:3001855/93‑1 (MQ=255)

       gagaTCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATa                                                                                  >  1:1140777/1‑93 (MQ=255)

       gagaTCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATa                                                                                  >  1:3693037/1‑93 (MQ=255)

       gagaTCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATa                                                                                  <  1:3227788/93‑1 (MQ=255)

       gagaTCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATa                                                                                  >  1:2713458/1‑93 (MQ=255)

       gagaTCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGATTTCATa                                                                                  >  1:3575618/1‑93 (MQ=255)

        agaTCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATaa                                                                                 >  1:1952161/1‑93 (MQ=255)

        agaTCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAACAAATGTTTCTGGAAAGGCTTTCATat                                                                                 >  1:1690460/1‑92 (MQ=255)

        acaTCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATaa                                                                                 <  1:2439729/91‑1 (MQ=255)

         gaTCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAAt                                                                                >  2:93885/1‑93 (MQ=255)

         gaTCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAAt                                                                                <  1:1009515/93‑1 (MQ=255)

         gaTCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAAt                                                                                >  2:2574728/1‑93 (MQ=255)

          aTCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAAtt                                                                               >  2:2108411/1‑93 (MQ=255)

           tCCCTTTGCCATCTTCCTGGCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTa                                                                              >  2:4584573/1‑93 (MQ=255)

           tCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTa                                                                              >  2:1639233/1‑93 (MQ=255)

            cccTTTGCCATCTTCCTGCCAGGCACCTTCCTCCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTaa                                                                             <  1:1390686/93‑1 (MQ=255)

            cccTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTaa                                                                             <  1:1760144/93‑1 (MQ=255)

            cccTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTaa                                                                             >  1:3131362/1‑93 (MQ=255)

             ccTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAAc                                                                            <  2:2627042/93‑1 (MQ=255)

             ccTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAAc                                                                            <  2:3349558/93‑1 (MQ=255)

              cTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACt                                                                           >  2:3885774/1‑93 (MQ=255)

               tttGCCATTTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAACTGTTTCTGGAAAGGCTTTTATAATTAACtt                                                                          >  2:2885733/1‑92 (MQ=255)

               tttGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTc                                                                          <  2:194259/93‑1 (MQ=255)

                  gCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCtt                                                                       <  2:4528739/93‑1 (MQ=255)

                   ccATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCttt                                                                      >  2:1441225/1‑93 (MQ=255)

                    cATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCtttt                                                                     >  1:3996874/1‑93 (MQ=255)

                     aTCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTa                                                                    >  1:4648197/1‑93 (MQ=255)

                       cTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATc                                                                  <  2:603015/93‑1 (MQ=255)

                       cTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATc                                                                  <  2:1604540/93‑1 (MQ=255)

                       cTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATc                                                                  <  2:962375/93‑1 (MQ=255)

                       cTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATc                                                                  <  1:1881541/93‑1 (MQ=255)

                         tCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGt                                                                >  2:352372/1‑93 (MQ=255)

                          ccTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGtt                                                               <  2:4575671/93‑1 (MQ=255)

                           cTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTa                                                              <  2:2452576/93‑1 (MQ=255)

                            tGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAg                                                             <  1:3419739/93‑1 (MQ=255)

                            tGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAg                                                             <  1:3961425/93‑1 (MQ=255)

                             gCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGc                                                            <  1:977008/93‑1 (MQ=255)

                             gCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGc                                                            <  1:1581635/93‑1 (MQ=255)

                                 ggCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAat                                                        >  1:1823456/1‑93 (MQ=255)

                                    aCCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAatgat                                                     <  1:2046124/93‑1 (MQ=255)

                                     ccTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAatgatg                                                    <  1:2604328/93‑1 (MQ=255)

                                     ccTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAatgatg                                                    <  2:1324685/93‑1 (MQ=255)

                                      cTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATgg                                                   <  2:538393/93‑1 (MQ=255)

                                          cACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATaa                                               <  1:4003243/93‑1 (MQ=255)

                                          cACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATaa                                               >  1:78005/1‑93 (MQ=255)

                                            ccTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACa                                             <  2:3803119/93‑1 (MQ=255)

                                             cTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAg                                            >  1:606710/1‑93 (MQ=255)

                                              tGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGc                                           >  1:4464964/1‑93 (MQ=255)

                                              tGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGc                                           <  2:1019154/93‑1 (MQ=255)

                                               ggttGGGAGTTGGTGCCCCCCCCCAAAGAAATTGTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCg                                          <  2:361297/91‑1 (MQ=255)

                                               gATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCg                                          >  2:945766/1‑93 (MQ=255)

                                                 ttGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGt                                        >  2:2926534/1‑93 (MQ=255)

                                                    gCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGttta                                     >  2:4395927/1‑91 (MQ=255)

                                                    gCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCa                                     >  1:712817/1‑93 (MQ=255)

                                                     cAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCAc                                    <  1:558671/93‑1 (MQ=255)

                                                      aGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCAcc                                   >  2:1907843/1‑93 (MQ=255)

                                                      aGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCAcc                                   <  2:737094/93‑1 (MQ=255)

                                                       gCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCt                                  <  2:2819071/93‑1 (MQ=255)

                                                        cTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTg                                 <  2:3573614/93‑1 (MQ=255)

                                                           ttGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTgcgc                              <  2:638953/93‑1 (MQ=255)

                                                              cgccgcGCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTa                           <  1:2474782/93‑1 (MQ=255)

                                                              cgcCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTGTCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTa                           >  2:805142/1‑93 (MQ=255)

                                                               gcCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTAt                          >  2:294018/1‑93 (MQ=255)

                                                               gcCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTAt                          >  2:3310343/1‑93 (MQ=255)

                                                               gcCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTAt                          >  1:3090300/1‑93 (MQ=255)

                                                               gcCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTAt                          >  2:3722990/1‑93 (MQ=255)

                                                                ccACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATc                         >  1:2913714/1‑93 (MQ=255)

                                                                  aCCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTg                       >  1:2299287/1‑93 (MQ=255)

                                                                  aCCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTg                       >  1:2324819/1‑93 (MQ=255)

                                                                  aCCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTg                       >  2:4347467/1‑93 (MQ=255)

                                                                  aCCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTg                       >  2:792977/1‑93 (MQ=255)

                                                                     ccAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTgcgc                    >  2:2525139/1‑93 (MQ=255)

                                                                     ccAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTgcgc                    >  1:1897827/1‑93 (MQ=255)

                                                                     ccAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTgcgc                    >  2:1298197/1‑93 (MQ=255)

                                                                      cAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCgcc                   >  1:2118997/1‑93 (MQ=255)

                                                                       aaaGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCgccg                  >  2:2208481/1‑93 (MQ=255)

                                                                        aaGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCgccgt                 <  2:1508617/93‑1 (MQ=255)

                                                                         aGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCgccgtg                >  1:2234766/1‑93 (MQ=255)

                                                                         aGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCgccgtg                >  2:3986143/1‑93 (MQ=255)

                                                                          gAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCgccgtgc               >  1:1288972/1‑93 (MQ=255)

                                                                          gAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCgccgtgc               >  2:4178691/1‑93 (MQ=255)

                                                                            aaTGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCgccgtgccg             >  2:3444579/1‑93 (MQ=255)

                                                                            aaTGTTCCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCgccgtgccg             <  1:2155180/93‑1 (MQ=255)

                                                                              tGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGccgtgccgtg           <  1:3750016/93‑1 (MQ=255)

                                                                              tGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGccgtgccgtg           >  1:1643095/1‑93 (MQ=255)

                                                                              tGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGccgtgccgtg           <  2:3159735/93‑1 (MQ=255)

                                                                               gTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTgg          >  1:46922/1‑93 (MQ=255)

                                                                                tttCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTggg         <  1:2837411/93‑1 (MQ=255)

                                                                                 ttCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTgggg        >  1:3145119/1‑93 (MQ=255)

                                                                                  tCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGt       <  2:122864/93‑1 (MQ=255)

                                                                                    tGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTaa     >  2:3401715/1‑93 (MQ=255)

                                                                                    tGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTaa     >  2:3423264/1‑93 (MQ=255)

                                                                                    tGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTaa     >  1:4517146/1‑93 (MQ=255)

                                                                                      gAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAATa   >  2:4481811/1‑93 (MQ=255)

                                                                                      gAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAATa   <  1:3263282/93‑1 (MQ=255)

                                                                                       aaaGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAATAc  <  1:4241376/93‑1 (MQ=255)

                                                                                       aaaGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAATAc  <  2:4301750/93‑1 (MQ=255)

TGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAATAC  >  NC_000913/3901231‑3901410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAATACGTCC  >  NC_000913/3901228‑3901414

ATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAG                                                                                               <  SRR4302535.1512973/94‑1 (MQ=60)

 TCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGG                                                                                              >  SRR4302535.3585414/1‑94 (MQ=60)

   TGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCT                                                                                            <  SRR4302535.3852524/94‑1 (MQ=60)

   TGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCT                                                                                            <  SRR4302535.3832514/94‑1 (MQ=60)

    GAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTT                                                                                           <  SRR4302535.3275100/94‑1 (MQ=60)

    GAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGGTTCTGGAAAGGCTT                                                                                           >  SRR4302535.3855209/1‑94 (MQ=60)

     AGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTT                                                                                          <  SRR4302535.2046499/94‑1 (MQ=60)

       GTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCA                                                                                        <  SRR4302535.4129882/94‑1 (MQ=60)

        TCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCAT                                                                                       <  SRR4302535.3022898/94‑1 (MQ=60)

         CGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATA                                                                                      >  SRR4302535.1054990/1‑94 (MQ=60)

         CGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATA                                                                                      >  SRR4302535.3718599/1‑94 (MQ=60)

         CGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATA                                                                                      >  SRR4302535.3121459/1‑94 (MQ=60)

         CGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATA                                                                                      >  SRR4302535.2732845/1‑94 (MQ=60)

         CGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATA                                                                                      >  SRR4302535.1148907/1‑94 (MQ=60)

         CGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGATTTCATA                                                                                      >  SRR4302535.3600407/1‑94 (MQ=60)

          GAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAA                                                                                     >  SRR4302535.646826/1‑94 (MQ=60)

          GAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAA                                                                                     <  SRR4302535.3250158/94‑1 (MQ=60)

          GAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAA                                                                                     >  SRR4302535.1966210/1‑94 (MQ=60)

          GAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAACAAATGTTTCTGGAAAGGCTTTCATAT                                                                                     >  SRR4302535.1702693/1‑94 (MQ=60)

           AGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAAT                                                                                    <  SRR4302535.1911168/94‑1 (MQ=60)

           ACATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAAT                                                                                    <  SRR4302535.2457305/94‑1 (MQ=60)

            GATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATT                                                                                   <  SRR4302535.1016688/94‑1 (MQ=60)

              TCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAA                                                                                 >  SRR4302535.3153171/1‑94 (MQ=60)

               CCCTTTGCCATCTTCCTGCCAGGCACCTTCCTCCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAAC                                                                                <  SRR4302535.1400610/94‑1 (MQ=60)

               CCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAAC                                                                                <  SRR4302535.1772887/94‑1 (MQ=60)

               CCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAAC                                                                                <  SRR4302535.2082226/94‑1 (MQ=60)

                CCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACT                                                                               <  SRR4302535.996723/94‑1 (MQ=60)

                CCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACT                                                                               <  SRR4302535.2971844/94‑1 (MQ=60)

                 CTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTC                                                                              >  SRR4302535.4633128/1‑94 (MQ=60)

                     GCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTT                                                                          >  SRR4302535.611580/1‑94 (MQ=60)

                     GCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTT                                                                          <  SRR4302535.4548596/94‑1 (MQ=60)

                      CCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTT                                                                         >  SRR4302535.4024218/1‑94 (MQ=60)

                       CATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTA                                                                        >  SRR4302535.4679681/1‑94 (MQ=60)

                        ATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTAT                                                                       >  SRR4302535.2154101/1‑94 (MQ=60)

                        ATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTAT                                                                       <  SRR4302535.1702823/94‑1 (MQ=60)

                          CTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCG                                                                     <  SRR4302535.1895103/94‑1 (MQ=60)

                          CTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCG                                                                     <  SRR4302535.2347013/94‑1 (MQ=60)

                          CTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCG                                                                     >  SRR4302535.2337430/1‑94 (MQ=60)

                           TTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGT                                                                    <  SRR4302535.3033339/94‑1 (MQ=60)

                              CTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAG                                                                 <  SRR4302535.954682/94‑1 (MQ=60)

                               TGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGC                                                                <  SRR4302535.3443526/94‑1 (MQ=60)

                               TGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGC                                                                <  SRR4302535.1914597/94‑1 (MQ=60)

                               TGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGC                                                                <  SRR4302535.3988478/94‑1 (MQ=60)

                                GCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCG                                                               <  SRR4302535.1593051/94‑1 (MQ=60)

                                GCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCG                                                               <  SRR4302535.983945/94‑1 (MQ=60)

                                GCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCG                                                               <  SRR4302535.1632102/94‑1 (MQ=60)

                                  CAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAA                                                             >  SRR4302535.2810832/1‑94 (MQ=60)

                                  CAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAA                                                             <  SRR4302535.167642/94‑1 (MQ=60)

                                   AGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAACTGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAAT                                                            >  SRR4302535.2283298/1‑94 (MQ=60)

                                   AGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAAT                                                            >  SRR4302535.3696278/1‑94 (MQ=60)

                                   AGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAAT                                                            >  SRR4302535.1836624/1‑94 (MQ=60)

                                      CACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGAT                                                         >  SRR4302535.485622/1‑94 (MQ=60)

                                       ACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATG                                                        <  SRR4302535.2060861/94‑1 (MQ=60)

                                       ACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATG                                                        <  SRR4302535.3950395/94‑1 (MQ=60)

                                       ACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATG                                                        >  SRR4302535.2982883/1‑94 (MQ=60)

                                        CCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGG                                                       <  SRR4302535.2623024/94‑1 (MQ=60)

                                        CCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGG                                                       <  SRR4302535.1501236/94‑1 (MQ=60)

                                        CCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGG                                                       >  SRR4302535.3751736/1‑94 (MQ=60)

                                            CCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAA                                                   >  SRR4302535.78564/1‑94 (MQ=60)

                                            CCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAA                                                   >  SRR4302535.2307750/1‑94 (MQ=60)

                                             CACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAAC                                                  <  SRR4302535.4030625/94‑1 (MQ=60)

                                               CCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAG                                                <  SRR4302535.4013242/94‑1 (MQ=60)

                                               CCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAG                                                >  SRR4302535.610946/1‑94 (MQ=60)

                                               CCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAG                                                <  SRR4302535.1797268/94‑1 (MQ=60)

                                                CTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGC                                               >  SRR4302535.4495587/1‑94 (MQ=60)

                                                CTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGC                                               <  SRR4302535.4706518/94‑1 (MQ=60)

                                                 TGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCG                                              <  SRR4302535.3232916/94‑1 (MQ=60)

                                                   ATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGT                                            >  SRR4302535.2381632/1‑94 (MQ=60)

                                                   ATGGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGT                                            <  SRR4302535.4384808/94‑1 (MQ=60)

                                                     TGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTC                                          <  SRR4302535.2182359/94‑1 (MQ=60)

                                                     TGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTC                                          <  SRR4302535.2469101/94‑1 (MQ=60)

                                                      GGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCA                                         <  SRR4302535.603117/94‑1 (MQ=60)

                                                      GGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCA                                         >  SRR4302535.717820/1‑94 (MQ=60)

                                                        CAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACC                                       <  SRR4302535.562577/94‑1 (MQ=60)

                                                         AGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCT                                      >  SRR4302535.3236537/1‑94 (MQ=60)

                                                           CTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGC                                    <  SRR4302535.2494901/94‑1 (MQ=60)

                                                           CTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGC                                    >  SRR4302535.1690505/1‑94 (MQ=60)

                                                           CTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGC                                    >  SRR4302535.2649407/1‑94 (MQ=60)

                                                            TGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCG                                   <  SRR4302535.4167491/94‑1 (MQ=60)

                                                            TGTTGCGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCG                                   >  SRR4302535.729926/1‑94 (MQ=60)

                                                                 CGCCGCGCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTAT                              <  SRR4302535.2492630/94‑1 (MQ=60)

                                                                 CGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTAT                              <  SRR4302535.1450736/94‑1 (MQ=60)

                                                                 CGCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTAT                              >  SRR4302535.3111822/1‑94 (MQ=60)

                                                                  GCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATC                             >  SRR4302535.3382282/1‑94 (MQ=60)

                                                                  GCCACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATC                             >  SRR4302535.2934295/1‑94 (MQ=60)

                                                                    CACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTG                           >  SRR4302535.2341452/1‑94 (MQ=60)

                                                                    CACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTG                           >  SRR4302535.2593189/1‑94 (MQ=60)

                                                                    CACCCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTG                           >  SRR4302535.2315738/1‑94 (MQ=60)

                                                                       CCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTATCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGC                        >  SRR4302535.1810699/1‑94 (MQ=60)

                                                                       CCCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGC                        >  SRR4302535.1911479/1‑94 (MQ=60)

                                                                        CCAAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCC                       >  SRR4302535.2134281/1‑94 (MQ=60)

                                                                          AAAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGT                     <  SRR4302535.2168152/94‑1 (MQ=60)

                                                                           AAGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTG                    >  SRR4302535.2250663/1‑94 (MQ=60)

                                                                            AGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGC                   >  SRR4302535.1298155/1‑94 (MQ=60)

                                                                            AGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGC                   >  SRR4302535.4087470/1‑94 (MQ=60)

                                                                            AGAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGC                   >  SRR4302535.4024694/1‑94 (MQ=60)

                                                                             GAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCC                  >  SRR4302535.4291688/1‑94 (MQ=60)

                                                                             GAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCC                  >  SRR4302535.3112645/1‑94 (MQ=60)

                                                                             GAAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCC                  >  SRR4302535.4501441/1‑94 (MQ=60)

                                                                              AAATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCG                 >  SRR4302535.3713301/1‑94 (MQ=60)

                                                                               AATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGT                >  SRR4302535.334057/1‑94 (MQ=60)

                                                                               AATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGT                <  SRR4302535.4088784/94‑1 (MQ=60)

                                                                               AATGTTCCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGT                <  SRR4302535.2170665/94‑1 (MQ=60)

                                                                                ATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTG               >  SRR4302535.2857379/1‑94 (MQ=60)

                                                                                ATGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTG               >  SRR4302535.1654973/1‑94 (MQ=60)

                                                                                 TGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGG              >  SRR4302535.47277/1‑94 (MQ=60)

                                                                                 TGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGG              >  SRR4302535.2036331/1‑94 (MQ=60)

                                                                                 TGTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGG              <  SRR4302535.3775961/94‑1 (MQ=60)

                                                                                  GTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCTCTTATCTGCGCCGTGCCGTGGG             <  SRR4302535.3999374/94‑1 (MQ=60)

                                                                                  GTTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGG             >  SRR4302535.1900999/1‑94 (MQ=60)

                                                                                   TTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGG            >  SRR4302535.3166992/1‑94 (MQ=60)

                                                                                   TTTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGG            <  SRR4302535.2857546/94‑1 (MQ=60)

                                                                                    TTCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGT           <  SRR4302535.1275876/94‑1 (MQ=60)

                                                                                     TCTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTA          <  SRR4302535.2896771/94‑1 (MQ=60)

                                                                                      CTGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAA         >  SRR4302535.4548108/1‑94 (MQ=60)

                                                                                       TGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAAT        <  SRR4302535.3557449/94‑1 (MQ=60)

                                                                                       TGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAAT        >  SRR4302535.503251/1‑94 (MQ=60)

                                                                                       TGGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAAT        >  SRR4302535.1882612/1‑94 (MQ=60)

                                                                                        GGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAATA       >  SRR4302535.1242737/1‑94 (MQ=60)

                                                                                        GGAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAATA       <  SRR4302535.1380311/94‑1 (MQ=60)

                                                                                         GAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAATAC      <  SRR4302535.3165734/94‑1 (MQ=60)

                                                                                         GAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAATAC      <  SRR4302535.3285947/94‑1 (MQ=60)

                                                                                         GAAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAATAC      <  SRR4302535.906140/94‑1 (MQ=60)

                                                                                          AAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAATACG     >  SRR4302535.4518766/1‑94 (MQ=60)

                                                                                          AAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAATACG     <  SRR4302535.4270405/94‑1 (MQ=60)

                                                                                          AAAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAATACG     >  SRR4302535.2023146/1‑94 (MQ=60)

                                                                                           AAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAATACGT    >  SRR4302535.3137883/1‑94 (MQ=60)

                                                                                           AAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAATACGT    <  SRR4302535.2566495/94‑1 (MQ=60)

                                                                                           AAGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAATACGT    >  SRR4302535.3780194/1‑94 (MQ=60)

                                                                                            AGGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAATACGTC   >  SRR4302535.1966220/1‑94 (MQ=60)

                                                                                             GGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAATACGTCC  >  SRR4302535.2673812/1‑94 (MQ=60)

                                                                                             GGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAATACGTCC  >  SRR4302535.1640589/1‑94 (MQ=60)

ATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCCGTGGGGTAATACGTCC  >  NC_000913/3901228‑3901414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`