Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,913,862 T→C D381G (GAT→GGT)  atpD ← membrane‑bound ATP synthase, F1 sector, beta‑subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,913,8620TC99.7% 909.9 / ‑4.8 286D381G (GAT→GGT) atpDmembrane‑bound ATP synthase, F1 sector, beta‑subunit
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (131/154);  total (132/154)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.79e-01

Only 100 of 295 total aligned reads displayed.

AATACTTCTGCCACGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGGTGTCGTAGTGTTCCTGA  >  NC_000913/3913772‑3913951
                                                                                          |                                                                                         
 aTACTTCTGCCACGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCa                                                                                        <  2:577760/93‑1 (MQ=255)
 aTACTTCTGCCACGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCa                                                                                        >  2:3753006/1‑93 (MQ=255)
    gttCTGCCACGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATAc                                                                                     <  1:2046335/92‑1 (MQ=255)
     ttCTGCCACGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATAcc                                                                                    <  1:403009/93‑1 (MQ=255)
     ttCTGCCACGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATAcc                                                                                    >  1:2910144/1‑93 (MQ=255)
     ttCTGCCACGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATAcc                                                                                    >  1:2870587/1‑93 (MQ=255)
     ttCTGCCACGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATAcc                                                                                    >  1:2198710/1‑93 (MQ=255)
           cACGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGGGCTACCACCAGTTTGTCTTCTTCAGACAGCTAACCCATACCCAGGAt                                                                              >  2:1761327/1‑93 (MQ=255)
           cACGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGAt                                                                              >  1:4531619/1‑93 (MQ=255)
           cACGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGAt                                                                              >  2:2737786/1‑93 (MQ=255)
           cACGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGAt                                                                              >  2:280918/1‑93 (MQ=255)
            aCGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATg                                                                             <  2:2202547/93‑1 (MQ=255)
             cGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATgg                                                                            >  1:1201521/1‑93 (MQ=255)
             cGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATgg                                                                            <  1:4283635/93‑1 (MQ=255)
             cGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATgg                                                                            <  2:3589300/93‑1 (MQ=255)
             cGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATgg                                                                            <  2:4413209/93‑1 (MQ=255)
              gaagaaCGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGc                                                                           <  2:4019133/93‑1 (MQ=255)
              gaagaaCGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGc                                                                           >  1:668999/1‑93 (MQ=255)
                agaaCGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCga                                                                         <  1:1641446/93‑1 (MQ=255)
                 gaaCGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCgat                                                                        <  2:2098035/93‑1 (MQ=255)
                 gaaCGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCgat                                                                        <  1:1777236/93‑1 (MQ=255)
                  aaCGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGTTGGCgatg                                                                       >  2:2357476/1‑93 (MQ=255)
                  aaCGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCgatg                                                                       >  1:3356064/1‑93 (MQ=255)
                   aCGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCgatga                                                                      >  1:542290/1‑93 (MQ=255)
                   aCGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCgatga                                                                      <  1:3018689/93‑1 (MQ=255)
                   aCGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCgatga                                                                      <  1:3915044/93‑1 (MQ=255)
                     ggCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGatgatg                                                                    <  2:2327366/93‑1 (MQ=255)
                      gCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGTTGGCGATGATGt                                                                   <  2:4303886/93‑1 (MQ=255)
                      gCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGt                                                                   <  2:3551446/93‑1 (MQ=255)
                        tGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCt                                                                 <  1:3762833/93‑1 (MQ=255)
                         gggACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCtt                                                                >  1:4114402/1‑93 (MQ=255)
                          ggACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCttt                                                               <  1:911699/93‑1 (MQ=255)
                           gACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTc                                                              <  2:1905787/93‑1 (MQ=255)
                           gACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTc                                                              <  2:2042749/93‑1 (MQ=255)
                            aCAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCa                                                             >  2:488341/1‑93 (MQ=255)
                             cAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAg                                                            <  2:2891854/93‑1 (MQ=255)
                              aGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGt                                                           <  2:216733/93‑1 (MQ=255)
                               ggAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGtt                                                          <  2:133983/93‑1 (MQ=255)
                               ggAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGtt                                                          >  1:2457392/1‑93 (MQ=255)
                                gAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGACGGCGATGATGTCTTTCAGTTc                                                         <  1:1319326/93‑1 (MQ=255)
                                 aaGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTcc                                                        >  2:1733601/1‑93 (MQ=255)
                                     gcTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGAt                                                    <  1:2714846/93‑1 (MQ=255)
                                     gcTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCGTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGAt                                                    <  1:1698946/93‑1 (MQ=255)
                                      cTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATa                                                   >  2:1386599/1‑93 (MQ=255)
                                       tGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACATTTCACCCATCCCCAGGATGGCGATGATGTCTTTCAGTTCCTGATaa                                                  <  2:4523662/93‑1 (MQ=255)
                                       tGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATaa                                                  >  2:338346/1‑93 (MQ=255)
                                       tGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATaa                                                  >  1:3109538/1‑93 (MQ=255)
                                       tGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATaa                                                  <  2:2484274/93‑1 (MQ=255)
                                        ggATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAAc                                                 <  2:1469907/93‑1 (MQ=255)
                                        ggATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAAc                                                 >  1:4311014/1‑93 (MQ=255)
                                         gATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACg                                                >  1:72242/1‑93 (MQ=255)
                                          aTCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGt                                               >  2:668156/1‑93 (MQ=255)
                                          aTCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGt                                               <  2:195039/93‑1 (MQ=255)
                                           tCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGtt                                              >  1:2748466/1‑93 (MQ=255)
                                           tCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGtt                                              <  2:1640564/93‑1 (MQ=255)
                                           tCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGtt                                              >  2:4070132/1‑93 (MQ=255)
                                             ttACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGc                                            >  1:3448970/1‑93 (MQ=255)
                                             ttACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGc                                            <  2:3469132/93‑1 (MQ=255)
                                              tACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATAGCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCa                                           <  1:3293104/93‑1 (MQ=255)
                                              tACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCa                                           >  2:2524787/1‑93 (MQ=255)
                                               aCGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAg                                          <  2:863491/93‑1 (MQ=255)
                                               aCGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAg                                          <  2:3147615/93‑1 (MQ=255)
                                                 gAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGa                                        <  2:3240983/93‑1 (MQ=255)
                                                  aGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGAt                                       <  1:948929/93‑1 (MQ=255)
                                                      cgcgCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGAc                                   <  1:1732544/93‑1 (MQ=255)
                                                       gcgcTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACt                                  >  1:1022803/1‑93 (MQ=255)
                                                         gcTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGa                                >  1:639567/1‑93 (MQ=255)
                                                         gcTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGa                                >  2:3314310/1‑93 (MQ=255)
                                                          cTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGaa                               <  2:975016/93‑1 (MQ=255)
                                                           tACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAAc                              >  2:2647442/1‑93 (MQ=255)
                                                           tACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAAc                              <  1:2006695/93‑1 (MQ=255)
                                                            accaccAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACg                             >  2:4148918/1‑93 (MQ=255)
                                                            accaccAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACg                             >  2:2371198/1‑93 (MQ=255)
                                                            accaccAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACg                             >  2:3052839/1‑93 (MQ=255)
                                                            accaccAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACg                             >  2:1710456/1‑93 (MQ=255)
                                                              caccaGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGcc                           >  2:682099/1‑93 (MQ=255)
                                                               accaGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCa                          <  2:2408775/93‑1 (MQ=255)
                                                               accaGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCa                          >  1:185320/1‑93 (MQ=255)
                                                               accaGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCa                          <  2:3356277/93‑1 (MQ=255)
                                                               accaGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCa                          <  2:2924733/93‑1 (MQ=255)
                                                                ccaTTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATTACGTTGCAGGCTGGACTGAACGGCAc                         >  1:2244589/1‑93 (MQ=255)
                                                                ccaGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCAc                         <  2:2990005/93‑1 (MQ=255)
                                                                  aGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCAcgc                       >  2:2271272/1‑93 (MQ=255)
                                                                  aGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCAcgc                       <  2:3897894/93‑1 (MQ=255)
                                                                   gTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCAcgcg                      >  1:3629284/1‑93 (MQ=255)
                                                                   gTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCAcgcg                      >  1:76401/1‑93 (MQ=255)
                                                                   gTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCAcgcg                      >  2:2471389/1‑93 (MQ=255)
                                                                        tcttctTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGgtg                 >  1:4643768/1‑93 (MQ=255)
                                                                         cttcttCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGgtgt                >  2:1546315/1‑93 (MQ=255)
                                                                           tcttcAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGGTGTCg              >  1:2381278/1‑93 (MQ=255)
                                                                             ttcAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGGTGTCGTa            >  1:1970197/1‑93 (MQ=255)
                                                                             ttcAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGGTGTCGTa            >  1:1290620/1‑93 (MQ=255)
                                                                                  aCAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGGTGTCGTAGTGtt       <  1:1525652/93‑1 (MQ=255)
                                                                                    aGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGGTGTCGTAGTGTTcc     <  1:4400466/93‑1 (MQ=255)
                                                                                    aGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGGTGTCGTAGTGTTcc     <  1:2981297/93‑1 (MQ=255)
                                                                                    aGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGGTGTCGTAGTGTTcc     >  1:3076417/1‑93 (MQ=255)
                                                                                     gTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGGTGTCGTAGTGTTCCt    <  1:1372291/93‑1 (MQ=255)
                                                                                      ttCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGGTGTCGTAGTGTTCCTg   >  2:1179909/1‑93 (MQ=255)
                                                                                      ttCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGGTGTCGTAGTGTTCCTg   >  2:127518/1‑93 (MQ=255)
                                                                                       tCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGGTGTCGTAGTGTTCCTGa  <  2:1881017/93‑1 (MQ=255)
                                                                                          |                                                                                         
AATACTTCTGCCACGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGGTGTCGTAGTGTTCCTGA  >  NC_000913/3913772‑3913951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTGAATACTTCTGCCACGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGGTGTCGTAGTGTTCCTGACCAA  >  NC_000913/3913769‑3913955
                                                                                             |                                                                                             
GTGAATACTTCTGCCACGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCAC                                                                                               >  SRR4302535.3603069/1‑94 (MQ=60)
GTGAATACTTCTGCCACGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCAC                                                                                               <  SRR4302535.4311494/94‑1 (MQ=60)
GTGAATACTTCTGCCACGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCAC                                                                                               <  SRR4302535.3242860/94‑1 (MQ=60)
 TGAATACTTCTGCCACGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACC                                                                                              <  SRR4302535.379065/94‑1 (MQ=60)
 TGAATACTTCTGCCACGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACC                                                                                              <  SRR4302535.1410369/94‑1 (MQ=60)
   AATACTTCTGCCACGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCA                                                                                            >  SRR4302535.4033885/1‑94 (MQ=60)
   AATACTTCTGCCACGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCA                                                                                            <  SRR4302535.1223278/94‑1 (MQ=60)
    ATACTTCTGCCACGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCAT                                                                                           <  SRR4302535.1729701/94‑1 (MQ=60)
    ATACTTCTGCCACGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCAT                                                                                           <  SRR4302535.1662213/94‑1 (MQ=60)
     TACTTCTGCCACGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTTAGACAGTTCACCCATA                                                                                          >  SRR4302535.1843366/1‑94 (MQ=60)
     TACTTCTGCCACGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATC                                                                                          >  SRR4302535.3873035/1‑94 (MQ=60)
      TCTTCTGCCACGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATAC                                                                                         <  SRR4302535.3136720/94‑1 (MQ=60)
      ACTTCTGCCACGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATAC                                                                                         <  SRR4302535.1977403/94‑1 (MQ=60)
       GTTCTGCCACGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACC                                                                                        <  SRR4302535.2061072/94‑1 (MQ=60)
       CTTCTGCCACGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACC                                                                                        >  SRR4302535.2890926/1‑94 (MQ=60)
       CTTCTGCCACGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACC                                                                                        >  SRR4302535.2930703/1‑94 (MQ=60)
       CTTCTGCCACGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACC                                                                                        >  SRR4302535.2214374/1‑94 (MQ=60)
        TTCTGCCACGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCC                                                                                       <  SRR4302535.1799812/94‑1 (MQ=60)
        TTCTGCCACGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCC                                                                                       <  SRR4302535.405833/94‑1 (MQ=60)
        TTCTGCCACGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACACATACCC                                                                                       >  SRR4302535.1724224/1‑94 (MQ=60)
          CTGCCACGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAG                                                                                     <  SRR4302535.1989184/94‑1 (MQ=60)
           TGCCACGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGG                                                                                    <  SRR4302535.2582513/94‑1 (MQ=60)
           TGCCACGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGG                                                                                    <  SRR4302535.1232297/94‑1 (MQ=60)
             CCACGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGAT                                                                                  >  SRR4302535.4562638/1‑94 (MQ=60)
             CCACGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGAT                                                                                  >  SRR4302535.223687/1‑94 (MQ=60)
              CACGAATAACGCCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCCGACAGTTCACCCATACCCAGGATG                                                                                 <  SRR4302535.2494675/94‑1 (MQ=60)
              CACGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATG                                                                                 <  SRR4302535.1365583/94‑1 (MQ=60)
              CACGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATG                                                                                 >  SRR4302535.4218864/1‑94 (MQ=60)
               ACGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGG                                                                                >  SRR4302535.1210132/1‑94 (MQ=60)
               ACGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGG                                                                                <  SRR4302535.148018/94‑1 (MQ=60)
                CGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGC                                                                               >  SRR4302535.673664/1‑94 (MQ=60)
                CGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGC                                                                               <  SRR4302535.4312941/94‑1 (MQ=60)
                CGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGC                                                                               <  SRR4302535.1659310/94‑1 (MQ=60)
                 GANGANNGNCTGGGACAGGNAGNNCTGGATCTTACGAGCACGCGCNACCACCAGTTTGTCTTCTTCAGACAGTTCANCCATACCCAGGATGGCG                                                                              <  SRR4302535.3677299/94‑1 (MQ=60)
                  AAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGA                                                                             >  SRR4302535.1303147/1‑94 (MQ=60)
                  AAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGA                                                                             >  SRR4302535.3117313/1‑94 (MQ=60)
                   AGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGAT                                                                            <  SRR4302535.854494/94‑1 (MQ=60)
                   AGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGAT                                                                            <  SRR4302535.1653310/94‑1 (MQ=60)
                    GAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATG                                                                           >  SRR4302535.3379431/1‑94 (MQ=60)
                    GAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATG                                                                           <  SRR4302535.1790106/94‑1 (MQ=60)
                     AACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGA                                                                          >  SRR4302535.546077/1‑94 (MQ=60)
                      ACGGCTGGGACAGGAAGCGCTGGATCTTGCGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGAT                                                                         <  SRR4302535.4497576/94‑1 (MQ=60)
                      ACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGAT                                                                         <  SRR4302535.2047316/94‑1 (MQ=60)
                      ACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGAT                                                                         <  SRR4302535.2980988/94‑1 (MQ=60)
                      ACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGAT                                                                         <  SRR4302535.625076/94‑1 (MQ=60)
                      ACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGAT                                                                         <  SRR4302535.3039834/94‑1 (MQ=60)
                      ACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGAT                                                                         <  SRR4302535.3941834/94‑1 (MQ=60)
                       CGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATG                                                                        <  SRR4302535.4135000/94‑1 (MQ=60)
                       CGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATG                                                                        <  SRR4302535.1305995/94‑1 (MQ=60)
                        GGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGT                                                                       <  SRR4302535.3613932/94‑1 (MQ=60)
                        GGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGT                                                                       >  SRR4302535.3404066/1‑94 (MQ=60)
                         GCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTC                                                                      <  SRR4302535.4418866/94‑1 (MQ=60)
                          GTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCT                                                                     <  SRR4302535.233318/94‑1 (MQ=60)
                           TGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTT                                                                    <  SRR4302535.3788829/94‑1 (MQ=60)
                           TGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTT                                                                    >  SRR4302535.4142563/1‑94 (MQ=60)
                            GGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTT                                                                   >  SRR4302535.3048426/1‑94 (MQ=60)
                            GGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTT                                                                   <  SRR4302535.1004461/94‑1 (MQ=60)
                             GGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTC                                                                  <  SRR4302535.918182/94‑1 (MQ=60)
                              GACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCA                                                                 <  SRR4302535.634712/94‑1 (MQ=60)
                              GACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCA                                                                 >  SRR4302535.725123/1‑94 (MQ=60)
                               ACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAG                                                                <  SRR4302535.1172191/94‑1 (MQ=60)
                                CAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGT                                                               <  SRR4302535.4546855/94‑1 (MQ=60)
                                 AGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTT                                                              >  SRR4302535.2475117/1‑94 (MQ=60)
                                 AGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTT                                                              <  SRR4302535.1765310/94‑1 (MQ=60)
                                 AGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTT                                                              >  SRR4302535.1463401/1‑94 (MQ=60)
                                 AGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTT                                                              >  SRR4302535.3603857/1‑94 (MQ=60)
                                  GGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTC                                                             >  SRR4302535.485809/1‑94 (MQ=60)
                                   GAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCC                                                            <  SRR4302535.752196/94‑1 (MQ=60)
                                   GAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGACGGCGATGATGTCTTTCAGTTCC                                                            <  SRR4302535.1328755/94‑1 (MQ=60)
                                    AAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCT                                                           <  SRR4302535.4597300/94‑1 (MQ=60)
                                      GCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGA                                                         <  SRR4302535.4329777/94‑1 (MQ=60)
                                       CGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGAT                                                        >  SRR4302535.2394169/1‑94 (MQ=60)
                                       CGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGAT                                                        <  SRR4302535.482441/94‑1 (MQ=60)
                                        GCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATA                                                       <  SRR4302535.2734241/94‑1 (MQ=60)
                                        GCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCGTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATA                                                       <  SRR4302535.1711224/94‑1 (MQ=60)
                                         CTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAA                                                      >  SRR4302535.3131186/1‑94 (MQ=60)
                                          TGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAAC                                                     >  SRR4302535.4340504/1‑94 (MQ=60)
                                          TGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAAC                                                     >  SRR4302535.2469817/1‑94 (MQ=60)
                                           GGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACG                                                    >  SRR4302535.199215/1‑94 (MQ=60)
                                           GGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACG                                                    >  SRR4302535.72768/1‑94 (MQ=60)
                                           GGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACG                                                    >  SRR4302535.4386245/1‑94 (MQ=60)
                                             ATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTT                                                  <  SRR4302535.1379347/94‑1 (MQ=60)
                                             ATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTT                                                  >  SRR4302535.2768059/1‑94 (MQ=60)
                                             ATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTT                                                  <  SRR4302535.2755345/94‑1 (MQ=60)
                                              TCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTG                                                 <  SRR4302535.4269887/94‑1 (MQ=60)
                                               CTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGC                                                >  SRR4302535.3472949/1‑94 (MQ=60)
                                                 TACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATAGCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAG                                              <  SRR4302535.3316001/94‑1 (MQ=60)
                                                  ACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGG                                             >  SRR4302535.1779451/1‑94 (MQ=60)
                                                   CGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGA                                            >  SRR4302535.684232/1‑94 (MQ=60)
                                                     AGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATG                                          <  SRR4302535.955651/94‑1 (MQ=60)
                                                     AGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATG                                          <  SRR4302535.830495/94‑1 (MQ=60)
                                                     AGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATG                                          >  SRR4302535.3453452/1‑94 (MQ=60)
                                                       CACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGA                                        >  SRR4302535.932166/1‑94 (MQ=60)
                                                       CACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGA                                        >  SRR4302535.3002959/1‑94 (MQ=60)
                                                         CGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACT                                      >  SRR4302535.1030068/1‑94 (MQ=60)
                                                         CGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACT                                      <  SRR4302535.1745066/94‑1 (MQ=60)
                                                          GCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTG                                     <  SRR4302535.3364474/94‑1 (MQ=60)
                                                           CGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGA                                    >  SRR4302535.3107359/1‑94 (MQ=60)
                                                           CGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGA                                    >  SRR4302535.644037/1‑94 (MQ=60)
                                                           CGCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGA                                    >  SRR4302535.2757662/1‑94 (MQ=60)
                                                            GCTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAA                                   <  SRR4302535.3311039/94‑1 (MQ=60)
                                                             CTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAAC                                  >  SRR4302535.2257210/1‑94 (MQ=60)
                                                             CTACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAAC                                  >  SRR4302535.280919/1‑94 (MQ=60)
                                                              TACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACG                                 <  SRR4302535.2021150/94‑1 (MQ=60)
                                                               ACCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGC                                >  SRR4302535.160918/1‑94 (MQ=60)
                                                                CCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCC                               >  SRR4302535.3649991/1‑94 (MQ=60)
                                                                CCACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCC                               >  SRR4302535.759757/1‑94 (MQ=60)
                                                                 CACCAGTTTGTCTTCTTCAGACAGTTCCCCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCA                              <  SRR4302535.670689/94‑1 (MQ=60)
                                                                 CACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCA                              >  SRR4302535.186598/1‑94 (MQ=60)
                                                                 CACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCA                              >  SRR4302535.2116115/1‑94 (MQ=60)
                                                                  ACCATTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATTACGTTGCAGGCTGGACTGAACGGCAC                             >  SRR4302535.2260552/1‑94 (MQ=60)
                                                                  ACCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCAC                             >  SRR4302535.373838/1‑94 (MQ=60)
                                                                   CCAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACG                            <  SRR4302535.3832859/94‑1 (MQ=60)
                                                                    CAGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGC                           >  SRR4302535.3025894/1‑94 (MQ=60)
                                                                     AGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCG                          >  SRR4302535.76955/1‑94 (MQ=60)
                                                                     AGTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCG                          >  SRR4302535.3654440/1‑94 (MQ=60)
                                                                      GTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAATGTTGCAGGATGGACTGAACGCCACGCGC                         >  SRR4302535.2907462/1‑94 (MQ=60)
                                                                      GTTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGC                         >  SRR4302535.331816/1‑94 (MQ=60)
                                                                       TTTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCG                        >  SRR4302535.1593306/1‑94 (MQ=60)
                                                                        TTGTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGG                       >  SRR4302535.4572743/1‑94 (MQ=60)
                                                                          GTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGCTGGACTGAACGCCACGCGCGGTG                     >  SRR4302535.3671848/1‑94 (MQ=60)
                                                                          GTCTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGGTG                     >  SRR4302535.4675242/1‑94 (MQ=60)
                                                                            CTTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGGTGTC                   >  SRR4302535.2860330/1‑94 (MQ=60)
                                                                             TTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGGTGTCG                  >  SRR4302535.3091055/1‑94 (MQ=60)
                                                                             TTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGGTGTCG                  >  SRR4302535.2398378/1‑94 (MQ=60)
                                                                             TTCTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGGTGTCG                  >  SRR4302535.4130728/1‑94 (MQ=60)
                                                                               CTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGGTGTCGTA                >  SRR4302535.1984390/1‑94 (MQ=60)
                                                                               CTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGGTGTCGTA                >  SRR4302535.1299820/1‑94 (MQ=60)
                                                                               CTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGGTGTCGTA                >  SRR4302535.4324126/1‑94 (MQ=60)
                                                                               CTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGGTGTCGTA                >  SRR4302535.2052270/1‑94 (MQ=60)
                                                                               CTTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGGTGTCGTA                <  SRR4302535.2102582/94‑1 (MQ=60)
                                                                                TTCAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGGTGTCGTAG               <  SRR4302535.3944814/94‑1 (MQ=60)
                                                                                  CAGACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGGTGTCGTAGTG             <  SRR4302535.1973671/94‑1 (MQ=60)
                                                                                     ACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGGTGTCGTAGTGTTC          <  SRR4302535.1536644/94‑1 (MQ=60)
                                                                                     ACAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGGTGTCGTAGTGTTC          >  SRR4302535.2428657/1‑94 (MQ=60)
                                                                                      CAGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGGTGTCGTAGTGTTCC         >  SRR4302535.3097879/1‑94 (MQ=60)
                                                                                       AGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGGTGTCGTAGTGTTCCT        <  SRR4302535.4430581/94‑1 (MQ=60)
                                                                                       AGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGGTGTCGTAGTGTTCCT        >  SRR4302535.2970208/1‑94 (MQ=60)
                                                                                       AGTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGGTGTCGTAGTGTTCCT        <  SRR4302535.3002211/94‑1 (MQ=60)
                                                                                        GTTCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGGTGTCGTAGTGTTCCTG       <  SRR4302535.1382097/94‑1 (MQ=60)
                                                                                          TCACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGGTGTCGTAGTGTTCCTGAC     <  SRR4302535.920156/94‑1 (MQ=60)
                                                                                           CACCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGGTGTCGTAGTGTTCCTGACC    >  SRR4302535.1892450/1‑94 (MQ=60)
                                                                                             CCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGGTGTCGTAGTGTTCCTGACCAA  >  SRR4302535.15601/1‑94 (MQ=60)
                                                                                             CCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGGTGTCGTAGTGTTCCTGACCAA  <  SRR4302535.3279296/94‑1 (MQ=60)
                                                                                             CCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGGTGTCGTAGTGTTCCTGACCAA  >  SRR4302535.693755/1‑94 (MQ=60)
                                                                                             CCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGGTGTCGTAGTGTTCCTGACCAA  >  SRR4302535.106198/1‑94 (MQ=60)
                                                                                             |                                                                                             
GTGAATACTTCTGCCACGAAGAACGGCTGGGACAGGAAGCGCTGGATCTTACGAGCACGCGCTACCACCAGTTTGTCTTCTTCAGACAGTTCATCCATACCCAGGATGGCGATGATGTCTTTCAGTTCCTGATAACGTTGCAGGATGGACTGAACGCCACGCGCGGTGTCGTAGTGTTCCTGACCAA  >  NC_000913/3913769‑3913955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑