Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,922,785	G→A	L159L (CTC→CTT)	gidA ←	glucose‑inhibited division

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,922,785	0	G	A	97.6%	720.3 / 0.5	208	L159L (CTC→CTT)	gidA	glucose‑inhibited division
Reads supporting (aligned to +/- strand): ref base G (5/0); new base A (109/94); total (114/94)
Fisher's exact test for biased strand distribution p-value = 6.53e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.73e-01

Only 100 of 212 total aligned reads displayed.

TTCACGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACA  >  NC_000913/3922695‑3922875

ttCACGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATGCAGACCGATATGAATTTTACCGTCAAg                                                                                          >  1:882697/1‑93 (MQ=255)

ttCACGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAg                                                                                          <  2:3756295/93‑1 (MQ=255)

 tCACGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAgg                                                                                         >  1:3557374/1‑93 (MQ=255)

  cACGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGa                                                                                        <  1:2124386/93‑1 (MQ=255)

   aCGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGaa                                                                                       <  2:2469487/93‑1 (MQ=255)

       aaaCGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTc                                                                                   >  1:2237373/1‑93 (MQ=255)

       aaaCGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTc                                                                                   <  1:1766758/93‑1 (MQ=255)

       aaaCGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAAAGTc                                                                                   >  1:1345154/1‑93 (MQ=255)

            gCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAAc                                                                              <  2:535827/93‑1 (MQ=255)

            gCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAAc                                                                              <  2:1483802/93‑1 (MQ=255)

              gagaAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACgg                                                                            >  1:4290242/1‑93 (MQ=255)

               agaAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGt                                                                           >  1:2885844/1‑93 (MQ=255)

               agaAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGt                                                                           <  2:3210402/93‑1 (MQ=255)

                gaAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTg                                                                          >  1:113718/1‑93 (MQ=255)

                    gCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCa                                                                      >  1:648064/1‑93 (MQ=255)

                    gCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCa                                                                      <  1:629953/93‑1 (MQ=255)

                    gCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCa                                                                      >  2:1894335/1‑93 (MQ=255)

                      ggAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCacg                                                                    >  2:4256856/1‑93 (MQ=255)

                      ggAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCacg                                                                    >  1:3082384/1‑93 (MQ=255)

                       gAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCacga                                                                   <  1:4570144/93‑1 (MQ=255)

                        aaTGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCacgac                                                                  <  2:1011490/93‑1 (MQ=255)

                        aaTGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCacgac                                                                  >  2:3348699/1‑93 (MQ=255)

                         aTGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCacgacg                                                                 >  2:342060/1‑93 (MQ=255)

                          tGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACgg                                                                >  1:3033116/1‑93 (MQ=255)

                               ggcggATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTg                                                           <  2:4549707/93‑1 (MQ=255)

                                gcggATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTgg                                                          >  1:45900/1‑93 (MQ=255)

                                gcggATCACCAGCACGCCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTgg                                                          <  2:2262141/93‑1 (MQ=255)

                                 cggATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGc                                                         <  2:2409962/93‑1 (MQ=255)

                                  ggATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCa                                                        >  2:805275/1‑93 (MQ=255)

                                  ggATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCa                                                        >  1:3503530/1‑93 (MQ=255)

                                      cACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGa                                                    >  1:3636223/1‑93 (MQ=255)

                                       cccAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGaa                                                   <  2:4219477/92‑1 (MQ=255)

                                       aCCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGaa                                                   >  1:2606327/1‑93 (MQ=255)

                                       aCCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGaa                                                   >  1:2580668/1‑93 (MQ=255)

                                       aCCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGaa                                                   >  1:573664/1‑93 (MQ=255)

                                        ccAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAAc                                                  >  1:1274526/1‑93 (MQ=255)

                                          aGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACtt                                                >  1:2436669/1‑93 (MQ=255)

                                          aGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACtt                                                >  1:2867134/1‑93 (MQ=255)

                                           gCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTc                                               <  2:1605972/93‑1 (MQ=255)

                                           gCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTc                                               <  1:2540719/93‑1 (MQ=255)

                                            cACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCa                                              <  2:1122739/93‑1 (MQ=255)

                                            cACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCa                                              <  2:2964292/93‑1 (MQ=255)

                                            cACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCa                                              <  1:1230573/93‑1 (MQ=255)

                                             aCGGCCACCGCTGTAATTATCCAGGCCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAg                                             <  2:1207600/93‑1 (MQ=255)

                                              cGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTCCCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGt                                            <  2:3403812/93‑1 (MQ=255)

                                              cGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGt                                            >  2:4638522/1‑93 (MQ=255)

                                               ggCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTc                                           <  2:3947378/93‑1 (MQ=255)

                                               ggCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTc                                           <  2:1216826/93‑1 (MQ=255)

                                               cgCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTc                                           <  2:1068301/92‑1 (MQ=255)

                                                gCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGACTTTGGCACGGAACTTCAGTcc                                          >  1:2986179/1‑93 (MQ=255)

                                                 ccACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTccc                                         >  1:4072890/1‑93 (MQ=255)

                                                 ccACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTccc                                         >  2:1707966/1‑93 (MQ=255)

                                                    ccGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCAtt                                      >  2:2844724/1‑93 (MQ=255)

                                                     cGCTGTAATTNTCCAGACCGATATNANTTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCAttt                                     <  2:1907377/93‑1 (MQ=255)

                                                      gCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTg                                    >  1:232912/1‑93 (MQ=255)

                                                      gCTGTAATTATCCACACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTg                                    <  1:2056277/93‑1 (MQ=255)

                                                       cTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTgg                                   >  2:4609863/1‑93 (MQ=255)

                                                       cTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTgg                                   <  1:2175926/93‑1 (MQ=255)

                                                       cTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTgg                                   <  1:1858249/93‑1 (MQ=255)

                                                       cTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTgg                                   >  1:390320/1‑93 (MQ=255)

                                                        tGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTggg                                  >  1:730516/1‑93 (MQ=255)

                                                         gTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGCACTTCAGTCCCATTTGGGt                                 >  1:3121955/1‑93 (MQ=255)

                                                         gTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTggga                                 >  1:1125152/1‑92 (MQ=255)

                                                         gTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGt                                 <  1:3725505/93‑1 (MQ=255)

                                                         gTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGt                                 <  1:3609138/93‑1 (MQ=255)

                                                         gTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGt                                 >  2:2219547/1‑93 (MQ=255)

                                                         gTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGt                                 >  1:2586569/1‑93 (MQ=255)

                                                         gTAATTATCCAGACCGATATGAATTTTACCGGCAAGGAACGTCCCAACGGTGAGCACGACGGCGTTGGCACGGCACTTCATTCCCATTTGGGt                                 >  1:2957788/1‑93 (MQ=255)

                                                            aTTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCAGTTGGGTAAc                              >  2:3764168/1‑93 (MQ=255)

                                                               aTCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGc                           >  2:1555654/1‑93 (MQ=255)

                                                                tCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCa                          >  2:402216/1‑93 (MQ=255)

                                                                tCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCa                          <  2:102129/93‑1 (MQ=255)

                                                                 ccAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCAc                         <  2:1404342/93‑1 (MQ=255)

                                                                  cAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCAcc                        <  1:1649252/93‑1 (MQ=255)

                                                                    gACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGa                      <  1:4409580/93‑1 (MQ=255)

                                                                     aCCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGAc                     >  2:4483635/1‑93 (MQ=255)

                                                                      ccGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGAcc                    >  1:4614941/1‑93 (MQ=255)

                                                                       cGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCa                   <  2:2257315/93‑1 (MQ=255)

                                                                        gATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCAc                  >  2:2141356/1‑93 (MQ=255)

                                                                         atatGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCAcg                 >  1:1483342/1‑93 (MQ=255)

                                                                         atatGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCAcg                 >  1:857512/1‑93 (MQ=255)

                                                                         atatGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCAcg                 >  2:3655816/1‑93 (MQ=255)

                                                                         atatGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCAcg                 >  2:3508274/1‑93 (MQ=255)

                                                                         atatGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCAcg                 >  1:2183616/1‑93 (MQ=255)

                                                                          tatGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCAcgc                >  2:2679699/1‑93 (MQ=255)

                                                                          tatGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCAcgc                >  1:970660/1‑93 (MQ=255)

                                                                          tatGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCAcgc                <  2:2910572/93‑1 (MQ=255)

                                                                           atGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCAcgcg               >  1:1522843/1‑93 (MQ=255)

                                                                            tGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACGCGa              >  2:3175005/1‑93 (MQ=255)

                                                                            tGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGGAACAGCACCGACCACGCGa              >  2:692743/1‑93 (MQ=255)

                                                                              aaTTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATc            <  1:4094324/93‑1 (MQ=255)

                                                                              aaTTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATc            <  2:3592706/93‑1 (MQ=255)

                                                                               aTTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCg           <  1:1564460/93‑1 (MQ=255)

                                                                                 tttACCGTCAAGTAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGtt         <  2:1374363/93‑1 (MQ=255)

                                                                                   tACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGtttt       <  2:2536520/93‑1 (MQ=255)

                                                                                    aCCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTc      >  1:3198147/1‑93 (MQ=255)

                                                                                      cGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGa    <  1:443737/93‑1 (MQ=255)

                                                                                      cGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGa    <  1:4528829/93‑1 (MQ=255)

                                                                                       gTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGAc   >  2:635819/1‑93 (MQ=255)

                                                                                        tCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACa  >  1:2017048/1‑93 (MQ=255)

TTCACGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACA  >  NC_000913/3922695‑3922875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CAGTTCACGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATA  >  NC_000913/3922692‑3922878

CAGTTCACGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCA                                                                                               >  SRR4302535.4307987/1‑94 (MQ=60)

  GTTCACGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATGCAGACCGATATGAATTTTACCGTCAAG                                                                                             >  SRR4302535.888947/1‑94 (MQ=60)

  GTTCACGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAG                                                                                             >  SRR4302535.2428945/1‑94 (MQ=60)

  GTTCACGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAG                                                                                             >  SRR4302535.3578758/1‑94 (MQ=60)

  GTTCACGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAG                                                                                             >  SRR4302535.4640011/1‑94 (MQ=60)

  GTTCACGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAG                                                                                             <  SRR4302535.3792052/94‑1 (MQ=60)

   TTCACGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGG                                                                                            >  SRR4302535.3582047/1‑94 (MQ=60)

    TCACGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGA                                                                                           <  SRR4302535.641762/94‑1 (MQ=60)

     CACGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCATCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAA                                                                                          <  SRR4302535.2528483/94‑1 (MQ=60)

     CACGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAA                                                                                          <  SRR4302535.2139698/94‑1 (MQ=60)

         CAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTC                                                                                      >  SRR4302535.2253291/1‑94 (MQ=60)

         CAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAAAGTC                                                                                      >  SRR4302535.1354758/1‑94 (MQ=60)

          AAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCC                                                                                     <  SRR4302535.1779569/94‑1 (MQ=60)

           AACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCC                                                                                    >  SRR4302535.1764179/1‑94 (MQ=60)

                CGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGG                                                                               <  SRR4302535.1604831/94‑1 (MQ=60)

                CGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGG                                                                               >  SRR4302535.4319583/1‑94 (MQ=60)

                 GAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGT                                                                              >  SRR4302535.2906261/1‑94 (MQ=60)

                  AGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGCCCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTG                                                                             <  SRR4302535.2970852/94‑1 (MQ=60)

                  AGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTG                                                                             >  SRR4302535.114514/1‑94 (MQ=60)

                  AGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTG                                                                             >  SRR4302535.2197715/1‑94 (MQ=60)

                  AGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTG                                                                             >  SRR4302535.4102539/1‑94 (MQ=60)

                    AAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAG                                                                           <  SRR4302535.946954/94‑1 (MQ=60)

                     AAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTNAAGGAACGTCCCAACGGTGNGC                                                                          >  SRR4302535.3637616/1‑94 (MQ=60)

                      AGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCA                                                                         >  SRR4302535.652582/1‑94 (MQ=60)

                      AGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCA                                                                         <  SRR4302535.2173001/94‑1 (MQ=60)

                       GCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCAC                                                                        <  SRR4302535.634345/94‑1 (MQ=60)

                        CGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACG                                                                       <  SRR4302535.2917058/94‑1 (MQ=60)

                        CGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACG                                                                       >  SRR4302535.3103866/1‑94 (MQ=60)

                         GGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGA                                                                      >  SRR4302535.308631/1‑94 (MQ=60)

                          GAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGAC                                                                     <  SRR4302535.4601308/94‑1 (MQ=60)

                          GAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGAC                                                                     <  SRR4302535.1529195/94‑1 (MQ=60)

                            ATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGG                                                                   >  SRR4302535.3054349/1‑94 (MQ=60)

                            ATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGG                                                                   >  SRR4302535.1322626/1‑94 (MQ=60)

                              GGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCT                                                                 <  SRR4302535.3661313/94‑1 (MQ=60)

                                ACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTT                                                               >  SRR4302535.4690419/1‑94 (MQ=60)

                                ACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTT                                                               <  SRR4302535.857269/94‑1 (MQ=60)

                                 CGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTG                                                              <  SRR4302535.609202/94‑1 (MQ=60)

                                 CGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTG                                                              <  SRR4302535.2568692/94‑1 (MQ=60)

                                  GGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCTATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGG                                                             <  SRR4302535.4276707/94‑1 (MQ=60)

                                  GGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGG                                                             >  SRR4302535.4591864/1‑94 (MQ=60)

                                  GGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGG                                                             >  SRR4302535.46248/1‑94 (MQ=60)

                                    CGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCA                                                           <  SRR4302535.1722580/94‑1 (MQ=60)

                                    CGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCA                                                           >  SRR4302535.3527881/1‑94 (MQ=60)

                                    CGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCA                                                           >  SRR4302535.1236605/1‑94 (MQ=60)

                                     GGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCAC                                                          >  SRR4302535.842016/1‑94 (MQ=60)

                                       ATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGG                                                        >  SRR4302535.661743/1‑94 (MQ=60)

                                        TCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGA                                                       >  SRR4302535.3661421/1‑94 (MQ=60)

                                         CACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAA                                                      >  SRR4302535.2625031/1‑94 (MQ=60)

                                         CACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAA                                                      >  SRR4302535.2599207/1‑94 (MQ=60)

                                         CACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAA                                                      >  SRR4302535.577691/1‑94 (MQ=60)

                                         CACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAA                                                      >  SRR4302535.3016529/1‑94 (MQ=60)

                                          ACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAAC                                                     >  SRR4302535.1283624/1‑94 (MQ=60)

                                            CAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTT                                                   >  SRR4302535.2887443/1‑94 (MQ=60)

                                            CAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTT                                                   >  SRR4302535.2454226/1‑94 (MQ=60)

                                              GCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCA                                                 <  SRR4302535.2559020/94‑1 (MQ=60)

                                               CACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAG                                                <  SRR4302535.1239369/94‑1 (MQ=60)

                                                ACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGT                                               <  SRR4302535.1924633/94‑1 (MQ=60)

                                                  GGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGACTTTGGCACGGAACTTCAGTCC                                             >  SRR4302535.3007123/1‑94 (MQ=60)

                                                   GCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCC                                            >  SRR4302535.4100761/1‑94 (MQ=60)

                                                     CACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCAT                                          >  SRR4302535.3003063/1‑94 (MQ=60)

                                                     CACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCAT                                          >  SRR4302535.1785633/1‑94 (MQ=60)

                                                      ACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATT                                         <  SRR4302535.312967/94‑1 (MQ=60)

                                                       CCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTT                                        <  SRR4302535.2097626/94‑1 (MQ=60)

                                                        CGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTG                                       >  SRR4302535.4482595/1‑94 (MQ=60)

                                                        CGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTG                                       >  SRR4302535.234520/1‑94 (MQ=60)

                                                        CGCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTG                                       >  SRR4302535.3605921/1‑94 (MQ=60)

                                                         GCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGG                                      >  SRR4302535.393037/1‑94 (MQ=60)

                                                         GCTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGG                                      <  SRR4302535.2276073/94‑1 (MQ=60)

                                                         GCTGTAATTATCCACACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGG                                      <  SRR4302535.2071090/94‑1 (MQ=60)

                                                          CTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGG                                     >  SRR4302535.735647/1‑94 (MQ=60)

                                                          CTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGG                                     <  SRR4302535.1134882/94‑1 (MQ=60)

                                                          CTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGG                                     >  SRR4302535.4467486/1‑94 (MQ=60)

                                                          CTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGG                                     <  SRR4302535.2191481/94‑1 (MQ=60)

                                                          CTGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGG                                     <  SRR4302535.1871649/94‑1 (MQ=60)

                                                           TGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGCACTTCAGTCCCATTTGGGT                                    >  SRR4302535.3143697/1‑94 (MQ=60)

                                                           TGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGT                                    >  SRR4302535.2605146/1‑94 (MQ=60)

                                                           TGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGT                                    >  SRR4302535.3213019/1‑94 (MQ=60)

                                                           TGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGT                                    >  SRR4302535.4359539/1‑94 (MQ=60)

                                                           TGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGT                                    >  SRR4302535.1568046/1‑94 (MQ=60)

                                                           TGTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGA                                    >  SRR4302535.1133184/1‑94 (MQ=60)

                                                           TGTAATTATCCAGACCGATATGAATTTTACCGGCAAGGAACGTCCCAACGGTGAGCACGACGGCGTTGGCACGGCACTTCATTCCCATTTGGGT                                    >  SRR4302535.2978560/1‑94 (MQ=60)

                                                            GTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTA                                   <  SRR4302535.3634171/94‑1 (MQ=60)

                                                            GTAATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTA                                   <  SRR4302535.3751317/94‑1 (MQ=60)

                                                              AATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAAC                                 >  SRR4302535.3750974/1‑94 (MQ=60)

                                                              AATTATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAAC                                 >  SRR4302535.26115/1‑94 (MQ=60)

                                                                tgataCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAG                               <  SRR4302535.4135809/89‑1 (MQ=60)

                                                                 TATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGC                              >  SRR4302535.2050715/1‑94 (MQ=60)

                                                                 TATCCAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGC                              <  SRR4302535.4549497/94‑1 (MQ=60)

                                                                     CAGACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCG                          <  SRR4302535.1661181/94‑1 (MQ=60)

                                                                       GACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGAC                        <  SRR4302535.4439754/94‑1 (MQ=60)

                                                                       GACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGAC                        <  SRR4302535.2223984/94‑1 (MQ=60)

                                                                        ACCGATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACC                       >  SRR4302535.4646296/1‑94 (MQ=60)

                                                                           GATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACG                    >  SRR4302535.1493951/1‑94 (MQ=60)

                                                                           GATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACG                    >  SRR4302535.863557/1‑94 (MQ=60)

                                                                           GATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACG                    >  SRR4302535.2199188/1‑94 (MQ=60)

                                                                            ATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACGC                   >  SRR4302535.977549/1‑94 (MQ=60)

                                                                            ATATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACGC                   >  SRR4302535.461285/1‑94 (MQ=60)

                                                                             TATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACGCG                  >  SRR4302535.1533811/1‑94 (MQ=60)

                                                                              ATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACGCGA                 >  SRR4302535.1268219/1‑94 (MQ=60)

                                                                              ATGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACAGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACGCGA                 <  SRR4302535.3431962/94‑1 (MQ=60)

                                                                               TGAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTttgtaaaatgaccgaccccgcggg                >  SRR4302535.2932475/1‑70 (MQ=60)

                                                                                GAATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATC               >  SRR4302535.3773899/1‑94 (MQ=60)

                                                                                 AATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCG              <  SRR4302535.2600781/94‑1 (MQ=60)

                                                                                 AATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCG              <  SRR4302535.4122344/94‑1 (MQ=60)

                                                                                  ATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGT             >  SRR4302535.2896758/1‑94 (MQ=60)

                                                                                  ATTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGT             <  SRR4302535.1575756/94‑1 (MQ=60)

                                                                                   TTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTT            <  SRR4302535.45192/94‑1 (MQ=60)

                                                                                   TTTTACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTT            >  SRR4302535.281193/1‑94 (MQ=60)

                                                                                     TTACCGTCAAGGAACGTCCCAACGGTGAGCACGACTGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTT          <  SRR4302535.869083/94‑1 (MQ=60)

                                                                                      TACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTC         >  SRR4302535.3220277/1‑94 (MQ=60)

                                                                                       ACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCG        <  SRR4302535.2339823/94‑1 (MQ=60)

                                                                                       ACCGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCG        >  SRR4302535.4067523/1‑94 (MQ=60)

                                                                                         CGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGAC      <  SRR4302535.4559840/94‑1 (MQ=60)

                                                                                         CGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGAC      <  SRR4302535.446844/94‑1 (MQ=60)

                                                                                         CGTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGAA      <  SRR4302535.1401515/94‑1 (MQ=60)

                                                                                          GTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACA     >  SRR4302535.2031577/1‑94 (MQ=60)

                                                                                          GTCAAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACA     >  SRR4302535.4004689/1‑94 (MQ=60)

                                                                                             AAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATA  >  SRR4302535.4243742/1‑94 (MQ=60)

                                                                                             AAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATA  >  SRR4302535.1324918/1‑94 (MQ=60)

                                                                                             AAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATA  <  SRR4302535.4258775/94‑1 (MQ=60)

                                                                                             AAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTAAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATA  >  SRR4302535.339623/1‑94 (MQ=60)

CAGTTCACGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATA  >  NC_000913/3922692‑3922878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`