Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,929,618	A→G	T226A (ACG→GCG)	kup →	low affinity potassium transport system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,929,618	0	A	G	99.1%	669.3 / ‑3.3	216	T226A (ACG→GCG)	kup	low affinity potassium transport system
Reads supporting (aligned to +/- strand): ref base A (2/0); new base G (122/92); total (124/92)
Fisher's exact test for biased strand distribution p-value = 5.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.23e-01

Only 100 of 224 total aligned reads displayed.

CATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTG  >  NC_000913/3929528‑3929707

 aTGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCgg                                                                                        <  2:4098243/93‑1 (MQ=255)

 aTGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCgg                                                                                        >  1:704289/1‑93 (MQ=255)

  tGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCggg                                                                                       >  1:3685723/1‑93 (MQ=255)

   gcgcTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCgggg                                                                                      >  2:3809431/1‑93 (MQ=255)

     gcTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCgggggc                                                                                    >  1:1519535/1‑92 (MQ=255)

       tGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCg                                                                                  >  2:1407396/1‑93 (MQ=255)

        gAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGa                                                                                 >  2:293524/1‑93 (MQ=255)

         aaTCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAg                                                                                <  1:4343679/93‑1 (MQ=255)

         aaTCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAg                                                                                <  1:2834861/93‑1 (MQ=255)

         aaTCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAg                                                                                <  1:2688222/93‑1 (MQ=255)

          aTCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAgg                                                                               >  1:1482460/1‑93 (MQ=255)

             cAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGgcgc                                                                            >  2:477772/1‑93 (MQ=255)

             cAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGgcgc                                                                            <  2:4649690/93‑1 (MQ=255)

               aTGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTg                                                                          >  2:208144/1‑93 (MQ=255)

                 gtgGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTa                                                                        <  1:4205732/93‑1 (MQ=255)

                 gtgGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTa                                                                        >  1:3178100/1‑93 (MQ=255)

                    ggCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGc                                                                     >  1:3246229/1‑93 (MQ=255)

                       ggTGCTTTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGa                                                                  <  1:2108999/93‑1 (MQ=255)

                       ggTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGa                                                                  <  1:4613474/93‑1 (MQ=255)

                        gTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGat                                                                 <  2:3217333/93‑1 (MQ=255)

                          gCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGatat                                                               >  1:3730058/1‑93 (MQ=255)

                          gCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGatat                                                               >  2:34435/1‑93 (MQ=255)

                            aTTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGTGCTGTATGCTGATATgg                                                             >  1:4639329/1‑93 (MQ=255)

                            aTTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATgg                                                             <  2:1123636/93‑1 (MQ=255)

                            aTTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATgg                                                             >  2:3895942/1‑93 (MQ=255)

                            aTTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATgg                                                             <  1:1217449/93‑1 (MQ=255)

                            aTTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATgg                                                             <  2:3871196/93‑1 (MQ=255)

                             tttCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATggg                                                            >  2:1666539/1‑93 (MQ=255)

                             tttCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATggg                                                            >  1:4044054/1‑93 (MQ=255)

                             tttCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATggg                                                            <  2:2852890/93‑1 (MQ=255)

                             tttCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATggg                                                            >  2:683349/1‑93 (MQ=255)

                               tcttcCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGc                                                          >  1:259587/1‑93 (MQ=255)

                               tcttcCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGc                                                          <  1:4023001/93‑1 (MQ=255)

                                cttcCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCa                                                         >  1:4390740/1‑93 (MQ=255)

                                 ttcCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCAc                                                        >  2:2032093/1‑93 (MQ=255)

                                 ttcCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCAc                                                        <  2:391985/93‑1 (MQ=255)

                                 ttcCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCAc                                                        >  2:1024755/1‑93 (MQ=255)

                                  tcCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACt                                                       >  2:80260/1‑93 (MQ=255)

                                      tGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTgg                                                   <  1:4360505/93‑1 (MQ=255)

                                       gAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGt                                                  >  2:4343855/1‑93 (MQ=255)

                                       gAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGt                                                  <  1:4322521/93‑1 (MQ=255)

                                         aTACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCGCTTTGGTaa                                                >  1:1123035/1‑93 (MQ=255)

                                         aTACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTaa                                                >  2:3609482/1‑93 (MQ=255)

                                           aCAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGt                                              >  1:2153348/1‑93 (MQ=255)

                                            cAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGtt                                             <  2:2689182/93‑1 (MQ=255)

                                            cAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGtt                                             <  2:3405117/93‑1 (MQ=255)

                                            cAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGtt                                             >  1:3422002/1‑93 (MQ=255)

                                             aaaaCGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTc                                            >  2:1967586/1‑93 (MQ=255)

                                              aaaCGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTcc                                           >  1:4269382/1‑93 (MQ=255)

                                              aaaCGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTcc                                           <  1:136970/93‑1 (MQ=255)

                                              aaaCGGTTTCTTTTATTGCATTAGGGGCAGTGGGGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGGACTTTGGTAAGTTcc                                           >  1:2746731/1‑93 (MQ=255)

                                               aaCGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTccc                                          <  1:4471152/93‑1 (MQ=255)

                                               aaCGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTccc                                          <  1:2073447/93‑1 (MQ=255)

                                                aCGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCt                                         >  2:1163711/1‑93 (MQ=255)

                                                 cGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTa                                        <  2:4377009/93‑1 (MQ=255)

                                                  ggTTTCTTTTATTGCATTAGGGGCTGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTAt                                       >  2:4518830/1‑93 (MQ=255)

                                                  ggTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTAt                                       <  1:3485042/93‑1 (MQ=255)

                                                  ggTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTAt                                       >  2:638000/1‑93 (MQ=255)

                                                  ggTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTAt                                       <  1:1398378/93‑1 (MQ=255)

                                                  ggTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTAt                                       >  2:3152588/1‑93 (MQ=255)

                                                   gTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTAtt                                      >  1:1244721/1‑93 (MQ=255)

                                                   gTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTAtt                                      >  2:737270/1‑93 (MQ=255)

                                                   gTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTAtt                                      >  1:3752830/1‑93 (MQ=255)

                                                    tttCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTc                                     <  1:2876166/93‑1 (MQ=255)

                                                       cTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGcc                                  <  1:4550207/93‑1 (MQ=255)

                                                        ttttATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCt                                 >  2:2517136/1‑93 (MQ=255)

                                                          ttATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTgg                               <  2:4250728/93‑1 (MQ=255)

                                                           tATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGc                              >  1:4180011/1‑93 (MQ=255)

                                                           tATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGc                              <  1:3114812/93‑1 (MQ=255)

                                                           tATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGGCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGc                              >  1:1632744/1‑93 (MQ=255)

                                                             ttGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGt                            <  1:4154410/93‑1 (MQ=255)

                                                             ttGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGt                            >  2:3610773/1‑93 (MQ=255)

                                                              tGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTg                           >  1:800756/1‑93 (MQ=255)

                                                                cATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGt                         <  1:2181417/93‑1 (MQ=255)

                                                                cATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGt                         >  2:2956385/1‑93 (MQ=255)

                                                                cATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGt                         >  1:1639768/1‑93 (MQ=255)

                                                                  ttAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTc                       >  1:2498660/1‑93 (MQ=255)

                                                                  ttAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTc                       >  1:1726256/1‑93 (MQ=255)

                                                                    aGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCAc                     <  2:3562393/93‑1 (MQ=255)

                                                                      gggCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACcg                   <  2:3008811/93‑1 (MQ=255)

                                                                      gggCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACcg                   <  1:1328742/93‑1 (MQ=255)

                                                                       ggCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACcgt                  <  2:4416710/93‑1 (MQ=255)

                                                                          aGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACcgtcgt               >  1:4095974/1‑93 (MQ=255)

                                                                           gtggtgTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTa              >  2:3229478/1‑93 (MQ=255)

                                                                           gtggtgTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTa              >  2:2485133/1‑93 (MQ=255)

                                                                              gtgtTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTa           <  1:2413544/93‑1 (MQ=255)

                                                                              gtgtTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTa           >  2:3712886/1‑93 (MQ=255)

                                                                               tgttgtCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTAc          <  2:1814401/93‑1 (MQ=255)

                                                                                gttgtCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTAcc         >  1:618959/1‑93 (MQ=255)

                                                                                 ttgtCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTAcct        >  1:858159/1‑93 (MQ=255)

                                                                                 ttgtCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTAcct        >  1:1180263/1‑93 (MQ=255)

                                                                                   gtCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTAccttc      <  2:2606791/93‑1 (MQ=255)

                                                                                   gtCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTAccttc      <  1:407403/93‑1 (MQ=255)

                                                                                    tCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTAccttcc     <  2:1855284/93‑1 (MQ=255)

                                                                                    tCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTAccttcc     >  1:2640640/1‑93 (MQ=255)

                                                                                      gATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTAccttcctt   <  2:2117014/93‑1 (MQ=255)

                                                                                      gATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTAccttcctt   >  1:739695/1‑93 (MQ=255)

                                                                                      gATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTAccttcctt   >  1:3887994/1‑93 (MQ=255)

                                                                                      gATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTAccttcctt   >  1:1875269/1‑93 (MQ=255)

                                                                                       aTTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCGTTg  >  2:1705904/1‑93 (MQ=255)

CATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTG  >  NC_000913/3929528‑3929707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGAC  >  NC_000913/3929525‑3929709

CTGCATGCGCTGAATCCGATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTG                                                                                             <  SRR4302535.798551/94‑1 (MQ=60)

CTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTG                                                                                             <  SRR4302535.354067/94‑1 (MQ=60)

CTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTG                                                                                             >  SRR4302535.3265526/1‑94 (MQ=60)

 TGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGC                                                                                            <  SRR4302535.1685104/94‑1 (MQ=60)

   CATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGG                                                                                          <  SRR4302535.2077611/94‑1 (MQ=60)

   CATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGG                                                                                          >  SRR4302535.709235/1‑94 (MQ=60)

   CATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGG                                                                                          <  SRR4302535.4364325/94‑1 (MQ=60)

   CATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGG                                                                                          >  SRR4302535.3138207/1‑94 (MQ=60)

    ATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGG                                                                                         <  SRR4302535.2366169/94‑1 (MQ=60)

    ATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGG                                                                                         >  SRR4302535.3711223/1‑94 (MQ=60)

       CGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGC                                                                                      >  SRR4302535.1530479/1‑94 (MQ=60)

         CTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCG                                                                                    >  SRR4302535.2663594/1‑94 (MQ=60)

           GAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAG                                                                                  <  SRR4302535.1137760/94‑1 (MQ=60)

           GAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAG                                                                                  >  SRR4302535.1627539/1‑94 (MQ=60)

           GAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAG                                                                                  <  SRR4302535.3714735/94‑1 (MQ=60)

           GAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAG                                                                                  >  SRR4302535.4213416/1‑94 (MQ=60)

           GAATCCAATGTGGGCGGTGCATTTCTTACTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAG                                                                                  <  SRR4302535.1838274/94‑1 (MQ=60)

            AATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGG                                                                                 >  SRR4302535.1493061/1‑94 (MQ=60)

            AATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGG                                                                                 <  SRR4302535.2854977/94‑1 (MQ=60)

            AATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGG                                                                                 <  SRR4302535.2707433/94‑1 (MQ=60)

            AATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGG                                                                                 <  SRR4302535.4373398/94‑1 (MQ=60)

                CAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCT                                                                             >  SRR4302535.1366972/1‑94 (MQ=60)

                CAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCT                                                                             >  SRR4302535.4136856/1‑94 (MQ=60)

                CAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCT                                                                             >  SRR4302535.2624023/1‑94 (MQ=60)

                CAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCT                                                                             <  SRR4302535.727484/94‑1 (MQ=60)

                 AATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTG                                                                            >  SRR4302535.968778/1‑94 (MQ=60)

                  ATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGT                                                                           <  SRR4302535.4062054/94‑1 (MQ=60)

                   TGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTA                                                                          >  SRR4302535.3200082/1‑94 (MQ=60)

                    GTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTAT                                                                         <  SRR4302535.4234514/94‑1 (MQ=60)

                      GGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGC                                                                       >  SRR4302535.3268772/1‑94 (MQ=60)

                         CGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGA                                                                    >  SRR4302535.2019789/1‑94 (MQ=60)

                          GGTGCTTTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGAT                                                                   <  SRR4302535.2124208/94‑1 (MQ=60)

                          GGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGAT                                                                   <  SRR4302535.4644818/94‑1 (MQ=60)

                           GTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATA                                                                  <  SRR4302535.3485719/94‑1 (MQ=60)

                            TGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATAT                                                                 >  SRR4302535.657005/1‑94 (MQ=60)

                            TGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATAT                                                                 >  SRR4302535.3755888/1‑94 (MQ=60)

                             GCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATG                                                                >  SRR4302535.3977806/1‑94 (MQ=60)

                              CATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGTGCTGTATGCTGATATGG                                                               >  SRR4302535.4670789/1‑94 (MQ=60)

                              CATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGC                                                               >  SRR4302535.4534328/1‑94 (MQ=60)

                               ATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGG                                                              <  SRR4302535.1226183/94‑1 (MQ=60)

                               ATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGG                                                              >  SRR4302535.4071742/1‑94 (MQ=60)

                                TTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGG                                                             >  SRR4302535.1527318/1‑94 (MQ=60)

                                 TTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGC                                                            >  SRR4302535.261385/1‑94 (MQ=60)

                                  TCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCA                                                           >  SRR4302535.4420783/1‑94 (MQ=60)

                                  TCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCA                                                           <  SRR4302535.411999/94‑1 (MQ=60)

                                  TCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCA                                                           <  SRR4302535.4050524/94‑1 (MQ=60)

                                    TTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACT                                                         <  SRR4302535.4303/94‑1 (MQ=60)

                                     TCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTT                                                        >  SRR4302535.936575/1‑94 (MQ=60)

                                       CTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTG                                                      <  SRR4302535.443153/94‑1 (MQ=60)

                                       CTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTG                                                      <  SRR4302535.212922/94‑1 (MQ=60)

                                         TGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGT                                                    <  SRR4302535.4390348/94‑1 (MQ=60)

                                          GAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTA                                                   <  SRR4302535.4352091/94‑1 (MQ=60)

                                          GAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTA                                                   >  SRR4302535.2766671/1‑94 (MQ=60)

                                           AATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCGCTTTGGTAA                                                  >  SRR4302535.1131049/1‑94 (MQ=60)

                                           AATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAA                                                  <  SRR4302535.3782759/94‑1 (MQ=60)

                                            ATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAG                                                 >  SRR4302535.1258800/1‑94 (MQ=60)

                                             TACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGT                                                >  SRR4302535.2168824/1‑94 (MQ=60)

                                              ACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTT                                               <  SRR4302535.3259149/94‑1 (MQ=60)

                                              ACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTT                                               >  SRR4302535.3445804/1‑94 (MQ=60)

                                                AAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCC                                             >  SRR4302535.4298614/1‑94 (MQ=60)

                                                AAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGGGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGGACTTTGGTAAGTTCC                                             >  SRR4302535.2766311/1‑94 (MQ=60)

                                                 AAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCC                                            <  SRR4302535.137930/94‑1 (MQ=60)

                                                  AACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCT                                           <  SRR4302535.2088384/94‑1 (MQ=60)

                                                  AACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCT                                           <  SRR4302535.4501834/94‑1 (MQ=60)

                                                   actgtgtctgTTATTGCAGTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCTCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTA                                          <  SRR4302535.750147/84‑1 (MQ=60)

                                                   ACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTA                                          <  SRR4302535.2088819/94‑1 (MQ=60)

                                                   ACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTA                                          >  SRR4302535.4657429/1‑94 (MQ=60)

                                                    CGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTAT                                         >  SRR4302535.1201035/1‑94 (MQ=60)

                                                    CGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTAT                                         <  SRR4302535.1252570/94‑1 (MQ=60)

                                                     GGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATT                                        <  SRR4302535.3509239/94‑1 (MQ=60)

                                                     GGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATT                                        <  SRR4302535.4479303/94‑1 (MQ=60)

                                                     GGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATT                                        <  SRR4302535.1408359/94‑1 (MQ=60)

                                                     GGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATT                                        >  SRR4302535.3778801/1‑94 (MQ=60)

                                                     GGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATT                                        <  SRR4302535.4406212/94‑1 (MQ=60)

                                                     GGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATT                                        >  SRR4302535.1253602/1‑94 (MQ=60)

                                                      GTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTC                                       >  SRR4302535.762200/1‑94 (MQ=60)

                                                      GTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTC                                       <  SRR4302535.1588797/94‑1 (MQ=60)

                                                      GTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTC                                       >  SRR4302535.217820/1‑94 (MQ=60)

                                                      GTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTC                                       <  SRR4302535.499660/94‑1 (MQ=60)

                                                       TTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCG                                      <  SRR4302535.2896540/94‑1 (MQ=60)

                                                        TTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGC                                     >  SRR4302535.1647498/1‑94 (MQ=60)

                                                          CTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCT                                   <  SRR4302535.4581310/94‑1 (MQ=60)

                                                          CTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCT                                   >  SRR4302535.2371845/1‑94 (MQ=60)

                                                          CTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCT                                   >  SRR4302535.1454587/1‑94 (MQ=60)

                                                            TTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGG                                 <  SRR4302535.3355371/94‑1 (MQ=60)

                                                             TTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGC                                >  SRR4302535.4208618/1‑94 (MQ=60)

                                                             TTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGGCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGC                                >  SRR4302535.1644542/1‑94 (MQ=60)

                                                              TATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCG                               <  SRR4302535.3136499/94‑1 (MQ=60)

                                                                TTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTG                             <  SRR4302535.54503/94‑1 (MQ=60)

                                                                TTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTG                             >  SRR4302535.806384/1‑94 (MQ=60)

                                                                TTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTG                             <  SRR4302535.4182853/94‑1 (MQ=60)

                                                                 TGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGGTCCCTATTCGCCTGGCGTGG                            >  SRR4302535.167255/1‑94 (MQ=60)

                                                                  GCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGT                           >  SRR4302535.1651623/1‑94 (MQ=60)

                                                                   CATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTT                          >  SRR4302535.398818/1‑94 (MQ=60)

                                                                   CATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTT                          >  SRR4302535.2507145/1‑94 (MQ=60)

                                                                   CATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTT                          <  SRR4302535.2196978/94‑1 (MQ=60)

                                                                   CATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTT                          >  SRR4302535.274072/1‑94 (MQ=60)

                                                                   CATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTT                          >  SRR4302535.3623885/1‑94 (MQ=60)

                                                                   CATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTT                          <  SRR4302535.4159248/94‑1 (MQ=60)

                                                                   CATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTT                          <  SRR4302535.3768042/94‑1 (MQ=60)

                                                                    ATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTC                         >  SRR4302535.1738737/1‑94 (MQ=60)

                                                                    ATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTC                         >  SRR4302535.2516686/1‑94 (MQ=60)

                                                                     TTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCA                        >  SRR4302535.4080993/1‑94 (MQ=60)

                                                                      TAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCAC                       >  SRR4302535.4374564/1‑94 (MQ=60)

                                                                      TAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCAC                       >  SRR4302535.1397207/1‑94 (MQ=60)

                                                                       AGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACC                      <  SRR4302535.2574249/94‑1 (MQ=60)

                                                                       AGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACC                      <  SRR4302535.1376530/94‑1 (MQ=60)

                                                                         GGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGT                    >  SRR4302535.4307847/1‑94 (MQ=60)

                                                                         GGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGT                    <  SRR4302535.1338221/94‑1 (MQ=60)

                                                                          GGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTC                   >  SRR4302535.4596796/1‑94 (MQ=60)

                                                                            CAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGT                 >  SRR4302535.4124010/1‑94 (MQ=60)

                                                                              GTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTAT               >  SRR4302535.4299016/1‑94 (MQ=60)

                                                                                GGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTA             <  SRR4302535.3019078/94‑1 (MQ=60)

                                                                                 GTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTAC            <  SRR4302535.2430919/94‑1 (MQ=60)

                                                                                  TGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACC           >  SRR4302535.623260/1‑94 (MQ=60)

                                                                                  TGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACC           <  SRR4302535.2880142/94‑1 (MQ=60)

                                                                                   GTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCT          >  SRR4302535.1188688/1‑94 (MQ=60)

                                                                                   GTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCT          >  SRR4302535.3085599/1‑94 (MQ=60)

                                                                                   GTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCT          >  SRR4302535.864207/1‑94 (MQ=60)

                                                                                    TTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTT         >  SRR4302535.2676488/1‑94 (MQ=60)

                                                                                     TGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTC        <  SRR4302535.821027/94‑1 (MQ=60)

                                                                                      GTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCC       >  SRR4302535.2659557/1‑94 (MQ=60)

                                                                                      GTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCC       <  SRR4302535.410258/94‑1 (MQ=60)

                                                                                       TCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCT      >  SRR4302535.4191122/1‑94 (MQ=60)

                                                                                        CGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTT     >  SRR4302535.3914661/1‑94 (MQ=60)

                                                                                        CGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTT     <  SRR4302535.368507/94‑1 (MQ=60)

                                                                                        CGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTT     >  SRR4302535.744893/1‑94 (MQ=60)

                                                                                        CGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTT     >  SRR4302535.1888775/1‑94 (MQ=60)

                                                                                           TTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGAC  <  SRR4302535.4442698/94‑1 (MQ=60)

CTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGAC  >  NC_000913/3929525‑3929709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`