Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,934,774 A→G K291E (AAA→GAA)  rbsB → D‑ribose periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,934,7740AG99.3% 938.1 / NA 288K291E (AAA→GAA) rbsBD‑ribose periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (137/149);  minor base T (1/1);  total (138/150)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 295 total aligned reads displayed.

CTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAG  >  NC_000913/3934684‑3934863
                                                                                                |                                                                                         
cTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGgaa                                                                                         <  2:4197040/93‑1 (MQ=255)
cTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGgaa                                                                                         <  1:1875562/93‑1 (MQ=255)
 tACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAAc                                                                                        <  1:1078997/93‑1 (MQ=255)
 tACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAAc                                                                                        <  2:3159504/93‑1 (MQ=255)
   cccGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTg                                                                                      <  1:2742725/93‑1 (MQ=255)
   cccGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTg                                                                                      <  1:1948131/93‑1 (MQ=255)
    ccGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTgg                                                                                     <  2:4125982/93‑1 (MQ=255)
     cGATCAGATTGGCGAGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGTTCTGGAACTGgt                                                                                    <  2:2476496/93‑1 (MQ=255)
      gATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGgtt                                                                                   >  1:472949/1‑93 (MQ=255)
      gATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTCGAACTGgtt                                                                                   >  2:813404/1‑93 (MQ=255)
       aTCAGATGGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGgttg                                                                                  <  1:2471091/93‑1 (MQ=255)
        tCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGgttgt                                                                                 >  1:454494/1‑93 (MQ=255)
          aGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTa                                                                               <  2:3580876/93‑1 (MQ=255)
            aTTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAg                                                                             >  1:2874037/1‑93 (MQ=255)
             ttGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGc                                                                            <  1:4301656/93‑1 (MQ=255)
             ttGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGc                                                                            <  1:669049/93‑1 (MQ=255)
                gcgcGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCagt                                                                         >  2:523/1‑93 (MQ=255)
                gcgcGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCagt                                                                         >  1:1225599/1‑93 (MQ=255)
                  gcgAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCagtag                                                                       >  2:2208261/1‑93 (MQ=255)
                   cgAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCagtagt                                                                      >  1:3013984/1‑93 (MQ=255)
                   cgAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCagtagt                                                                      <  1:3549678/93‑1 (MQ=255)
                    gAAAGGCGTCGAAACCGCAGATAAAGTGCTTAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGtt                                                                     <  2:1605928/93‑1 (MQ=255)
                      aaGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGtttt                                                                   >  1:1534122/1‑93 (MQ=255)
                        ggCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTaa                                                                 >  2:4207410/1‑93 (MQ=255)
                           gTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTCGAACTGGCTGTTAAGCAGTAGttt                                                                    >  1:520252/1‑93 (MQ=255)
                           gTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCa                                                              >  1:705630/1‑93 (MQ=255)
                           gTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCa                                                              >  1:3529419/1‑93 (MQ=255)
                            tCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAg                                                             <  1:160997/93‑1 (MQ=255)
                            tCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAg                                                             <  2:2535399/93‑1 (MQ=255)
                            tCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAg                                                             >  1:3259916/1‑93 (MQ=255)
                              gAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGt                                                           >  1:246532/1‑93 (MQ=255)
                                aaCCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTg                                                         >  2:2934858/1‑93 (MQ=255)
                                aaCCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTg                                                         >  2:3282697/1‑93 (MQ=255)
                                  ccGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTa                                                       >  1:3836045/1‑93 (MQ=255)
                                  ccGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTa                                                       <  2:4616566/93‑1 (MQ=255)
                                   cGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTAt                                                      <  2:638050/93‑1 (MQ=255)
                                   cGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTAt                                                      >  2:3232685/1‑93 (MQ=255)
                                   cGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTAt                                                      <  1:4235500/93‑1 (MQ=255)
                                    gCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATg                                                     >  2:923234/1‑93 (MQ=255)
                                     cAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGa                                                    <  1:1012868/93‑1 (MQ=255)
                                     cAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGa                                                    >  1:3286694/1‑93 (MQ=255)
                                     cAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGa                                                    >  2:1418274/1‑93 (MQ=255)
                                     cAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGa                                                    <  2:436336/93‑1 (MQ=255)
                                      aGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGAc                                                   >  1:1989815/1‑93 (MQ=255)
                                        aTAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCt                                                 <  1:3219491/93‑1 (MQ=255)
                                         tAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTg                                                >  2:2849866/1‑93 (MQ=255)
                                          aaaGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGa                                               >  1:1421652/1‑93 (MQ=255)
                                             gTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATgg                                            >  2:688778/1‑93 (MQ=255)
                                              tGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGt                                           <  2:2953324/93‑1 (MQ=255)
                                              tGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGt                                           <  1:4662098/93‑1 (MQ=255)
                                              tGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGt                                           >  1:2675708/1‑93 (MQ=255)
                                              tGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGt                                           >  1:2359185/1‑93 (MQ=255)
                                                cTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGa                                         >  1:3953392/1‑93 (MQ=255)
                                                 tGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGAc                                        >  1:4080721/1‑93 (MQ=255)
                                                  gAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACa                                       >  1:1312703/1‑93 (MQ=255)
                                                    aaGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATa                                     >  1:3166804/1‑93 (MQ=255)
                                                    aaGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATa                                     >  1:3363763/1‑93 (MQ=255)
                                                     aGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATaa                                    >  2:2382737/1‑93 (MQ=255)
                                                      ggC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATaaa                                   <  1:2587588/93‑1 (MQ=255)
                                                       gC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAAt                                  <  1:9140/93‑1 (MQ=255)
                                                        c‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATa                                 <  1:805380/93‑1 (MQ=255)
                                                        c‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATa                                 <  1:1300840/93‑1 (MQ=255)
                                                               gagaAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATAc                                <  1:2849990/93‑1 (MQ=255)
                                                                agaAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACg                               >  2:172091/1‑93 (MQ=255)
                                                                 gaAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGt                              <  1:4015525/93‑1 (MQ=255)
                                                                  aaaGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTc                             <  2:3886137/93‑1 (MQ=255)
                                                                  aaaGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTc                             <  2:133911/93‑1 (MQ=255)
                                                                   aaGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCa                            >  2:3503160/1‑93 (MQ=255)
                                                                   aaGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCa                            <  1:3593758/93‑1 (MQ=255)
                                                                   aaGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCa                            >  2:2283439/1‑93 (MQ=255)
                                                                    aGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCAt                           <  2:3743217/93‑1 (MQ=255)
                                                                     gTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATc                          <  1:4370188/93‑1 (MQ=255)
                                                                       tCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGa                        >  2:1538388/1‑93 (MQ=255)
                                                                       tCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGa                        <  1:4487051/93‑1 (MQ=255)
                                                                        cAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGat                       <  1:2239343/93‑2 (MQ=255)
                                                                        cAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGat                       <  1:2122506/93‑2 (MQ=255)
                                                                         aGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGata                      <  2:1565817/93‑3 (MQ=255)
                                                                          ggCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAg                     >  1:1195176/1‑93 (MQ=255)
                                                                           gCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGa                    <  2:82494/93‑1 (MQ=255)
                                                                           gCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGa                    >  2:1068049/1‑93 (MQ=255)
                                                                            cTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGAt                   <  1:3062088/93‑1 (MQ=255)
                                                                            cTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGAt                   <  2:1964178/93‑1 (MQ=255)
                                                                             tAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGATg                  >  1:604025/1‑93 (MQ=255)
                                                                             tAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGATg                  <  2:4303568/93‑1 (MQ=255)
                                                                              aaGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGATGa                 >  2:2725137/1‑93 (MQ=255)
                                                                               aGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGATGaa                >  2:3395663/1‑93 (MQ=255)
                                                                               aGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGATGaa                <  2:444184/93‑1 (MQ=255)
                                                                                gTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGATGAAc               <  2:1220495/93‑1 (MQ=255)
                                                                                gTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGATGAAc               <  2:3943541/93‑1 (MQ=255)
                                                                                 tATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGATGAACg              <  2:466784/93‑1 (MQ=255)
                                                                                   tCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGCTGAACgtg            <  2:4571051/93‑1 (MQ=255)
                                                                                   tCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGATGAACgtg            >  2:1808632/1‑93 (MQ=255)
                                                                                    ccGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGATGAACgtgt           <  2:2775773/93‑1 (MQ=255)
                                                                                     cGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGATGAACGTGTa          >  1:3612175/1‑93 (MQ=255)
                                                                                     cGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGATGAACGTGTa          >  2:1902273/1‑93 (MQ=255)
                                                                                       gTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGATGAACGTGTAat        >  1:4597957/1‑93 (MQ=255)
                                                                                           aTCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGATGAACGTGTAATATaa    >  2:3022227/1‑93 (MQ=255)
                                                                                            tCTGTAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGATGAACGTGTAATATaaa   <  2:364958/93‑1 (MQ=255)
                                                                                            tCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGATGAACGTGTAATATaaa   >  1:1329757/1‑93 (MQ=255)
                                                                                            tCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGATGAACGTGTAATATaaa   >  2:3256828/1‑93 (MQ=255)
                                                                                                |                                                                                         
CTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAG  >  NC_000913/3934684‑3934863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAA  >  NC_000913/3934681‑3934867
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CAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGG                                                                                               <  SRR4302535.1744805/94‑1 (MQ=60)
 AGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGA                                                                                              >  SRR4302535.3753611/1‑94 (MQ=60)
   CTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAAC                                                                                            <  SRR4302535.1889069/94‑1 (MQ=60)
   CTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAAC                                                                                            >  SRR4302535.4631239/1‑94 (MQ=60)
   CTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAAC                                                                                            <  SRR4302535.2892562/94‑1 (MQ=60)
    TACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACT                                                                                           <  SRR4302535.106401/94‑1 (MQ=60)
    TACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACT                                                                                           <  SRR4302535.1086675/94‑1 (MQ=60)
    TACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACT                                                                                           <  SRR4302535.1678916/94‑1 (MQ=60)
     ACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTG                                                                                          <  SRR4302535.2465343/94‑1 (MQ=60)
      CCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGG                                                                                         <  SRR4302535.1962156/94‑1 (MQ=60)
      CCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGG                                                                                         <  SRR4302535.2762273/94‑1 (MQ=60)
        CGCTCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTT                                                                                       >  SRR4302535.2878864/1‑94 (MQ=60)
        CGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTT                                                                                       >  SRR4302535.476260/1‑94 (MQ=60)
         GATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGTAACTGGTTG                                                                                      >  SRR4302535.3647958/1‑94 (MQ=60)
         GATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTG                                                                                      <  SRR4302535.1834844/94‑1 (MQ=60)
         GATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTG                                                                                      >  SRR4302535.292623/1‑94 (MQ=60)
         GATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTG                                                                                      >  SRR4302535.533742/1‑94 (MQ=60)
          ATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGT                                                                                     >  SRR4302535.457679/1‑94 (MQ=60)
          ATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGT                                                                                     >  SRR4302535.2716056/1‑94 (MQ=60)
          ATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGT                                                                                     >  SRR4302535.457678/1‑94 (MQ=60)
          ATCAGATGGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGT                                                                                     <  SRR4302535.2488906/94‑1 (MQ=60)
           TCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTT                                                                                    <  SRR4302535.781987/94‑1 (MQ=60)
            CAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTA                                                                                   >  SRR4302535.2088423/1‑94 (MQ=60)
             AGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAA                                                                                  >  SRR4302535.3995714/1‑94 (MQ=60)
             AGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAA                                                                                  <  SRR4302535.1281657/94‑1 (MQ=60)
              GATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAG                                                                                 <  SRR4302535.2928619/94‑1 (MQ=60)
              GATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAG                                                                                 >  SRR4302535.2894402/1‑94 (MQ=60)
              GATTGGCGCGAAAGGCGTCAAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAG                                                                                 <  SRR4302535.4708028/94‑1 (MQ=60)
               ATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGC                                                                                >  SRR4302535.1572804/1‑94 (MQ=60)
                TTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCA                                                                               <  SRR4302535.4331087/94‑1 (MQ=60)
                TTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCA                                                                               <  SRR4302535.673715/94‑1 (MQ=60)
                 TGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAG                                                                              <  SRR4302535.456450/94‑1 (MQ=60)
                  GGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGT                                                                             <  SRR4302535.828633/94‑1 (MQ=60)
                  GGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGT                                                                             >  SRR4302535.1234366/1‑94 (MQ=60)
                    CGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAG                                                                           <  SRR4302535.4587995/94‑1 (MQ=60)
                    CGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAG                                                                           <  SRR4302535.1958130/94‑1 (MQ=60)
                    CGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAG                                                                           <  SRR4302535.3294738/94‑1 (MQ=60)
                     GCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGT                                                                          >  SRR4302535.3035111/1‑94 (MQ=60)
                      TGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTT                                                                         >  SRR4302535.3191279/1‑94 (MQ=60)
                      CGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTT                                                                         <  SRR4302535.3574315/94‑1 (MQ=60)
                      CGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTT                                                                         <  SRR4302535.1989843/94‑1 (MQ=60)
                      CGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTT                                                                         <  SRR4302535.486583/94‑1 (MQ=60)
                       GAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTT                                                                        <  SRR4302535.62862/94‑1 (MQ=60)
                       GAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTT                                                                        <  SRR4302535.553698/94‑1 (MQ=60)
                       GAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTT                                                                        <  SRR4302535.2686761/94‑1 (MQ=60)
                        AAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTT                                                                       >  SRR4302535.1310233/1‑94 (MQ=60)
                        AAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTT                                                                       >  SRR4302535.1545175/1‑94 (MQ=60)
                         AAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTA                                                                      <  SRR4302535.3220674/94‑1 (MQ=60)
                           GGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAAT                                                                    >  SRR4302535.2355130/1‑94 (MQ=60)
                           GGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAAT                                                                    <  SRR4302535.1332593/94‑1 (MQ=60)
                             CGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTCGAACTGGCTGTTAAGCAGTAGTTT                                                                        >  SRR4302535.523889/1‑94 (MQ=60)
                             CGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCA                                                                  <  SRR4302535.3555953/94‑1 (MQ=60)
                             CGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCA                                                                  <  SRR4302535.3100200/94‑1 (MQ=60)
                             CGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCA                                                                  >  SRR4302535.3553928/1‑94 (MQ=60)
                             CGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCA                                                                  <  SRR4302535.1988944/94‑1 (MQ=60)
                             CGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCA                                                                  >  SRR4302535.710586/1‑94 (MQ=60)
                             CGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCA                                                                  >  SRR4302535.3288454/1‑94 (MQ=60)
                              GTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAG                                                                 >  SRR4302535.3282571/1‑94 (MQ=60)
                              GTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAG                                                                 >  SRR4302535.1382231/1‑94 (MQ=60)
                              GTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAG                                                                 >  SRR4302535.4252968/1‑94 (MQ=60)
                               TCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGG                                                                <  SRR4302535.162125/94‑1 (MQ=60)
                                CGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGT                                                               >  SRR4302535.1396381/1‑94 (MQ=60)
                                CGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGT                                                               >  SRR4302535.248223/1‑94 (MQ=60)
                                CGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGT                                                               >  SRR4302535.4082933/1‑94 (MQ=60)
                                 GAACCCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTT                                                              <  SRR4302535.4353802/94‑1 (MQ=60)
                                  AAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTG                                                             >  SRR4302535.2847482/1‑94 (MQ=60)
                                    ACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTA                                                           >  SRR4302535.3862496/1‑94 (MQ=60)
                                    ACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTA                                                           >  SRR4302535.3915961/1‑94 (MQ=60)
                                     CCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTAT                                                          <  SRR4302535.504374/94‑1 (MQ=60)
                                      CGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATG                                                         <  SRR4302535.4264489/94‑1 (MQ=60)
                                       GCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGA                                                        >  SRR4302535.3309541/1‑94 (MQ=60)
                                        CAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGAC                                                       <  SRR4302535.1020070/94‑1 (MQ=60)
                                        CAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGAC                                                       >  SRR4302535.2004140/1‑94 (MQ=60)
                                        CAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGACGTTTTAATCAGGTTGTATGAC                                                       >  SRR4302535.69690/1‑94 (MQ=60)
                                         AGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACC                                                      >  SRR4302535.2582787/1‑94 (MQ=60)
                                          GATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCT                                                     <  SRR4302535.3602464/94‑1 (MQ=60)
                                           ATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTG                                                    <  SRR4302535.3241799/94‑1 (MQ=60)
                                            TAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGA                                                   >  SRR4302535.3263723/1‑94 (MQ=60)
                                            TAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGA                                                   >  SRR4302535.1431802/1‑94 (MQ=60)
                                            TAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGA                                                   >  SRR4302535.2642231/1‑94 (MQ=60)
                                             AAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGAT                                                  <  SRR4302535.4296782/94‑1 (MQ=60)
                                              AAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATG                                                 >  SRR4302535.3269961/1‑94 (MQ=60)
                                                GTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGT                                               >  SRR4302535.2376114/1‑94 (MQ=60)
                                                GTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGT                                               >  SRR4302535.2694852/1‑94 (MQ=60)
                                                 TGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTG                                              <  SRR4302535.4693626/94‑1 (MQ=60)
                                                 TGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTG                                              <  SRR4302535.1314093/94‑1 (MQ=60)
                                                  GCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGA                                             >  SRR4302535.3980381/1‑94 (MQ=60)
                                                   CTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGAC                                            >  SRR4302535.3315558/1‑94 (MQ=60)
                                                   CTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGAC                                            >  SRR4302535.121167/1‑94 (MQ=60)
                                                   CTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGAC                                            >  SRR4302535.4108634/1‑94 (MQ=60)
                                                    TGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACA                                           >  SRR4302535.1322086/1‑94 (MQ=60)
                                                      AAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATA                                         >  SRR4302535.3387168/1‑94 (MQ=60)
                                                      AAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATA                                         >  SRR4302535.4188869/1‑94 (MQ=60)
                                                      AAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATA                                         >  SRR4302535.3188748/1‑94 (MQ=60)
                                                        AGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAA                                       <  SRR4302535.1484642/94‑1 (MQ=60)
                                                         GGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAAT                                      <  SRR4302535.2557046/94‑1 (MQ=60)
                                                         GGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAAT                                      <  SRR4302535.2606169/94‑1 (MQ=60)
                                                          GC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATA                                     <  SRR4302535.9213/94‑1 (MQ=60)
                                                          GC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATA                                     <  SRR4302535.4326536/94‑1 (MQ=60)
                                                           C‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATAC                                    <  SRR4302535.1310117/94‑1 (MQ=60)
                                                           C‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATAC                                    >  SRR4302535.3507022/1‑94 (MQ=60)
                                                           C‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATAC                                    <  SRR4302535.811031/94‑1 (MQ=60)
                                                                  GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACG                                   >  SRR4302535.1164714/1‑94 (MQ=60)
                                                                  GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACG                                   <  SRR4302535.2870181/94‑1 (MQ=60)
                                                                  GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACG                                   <  SRR4302535.4573100/94‑1 (MQ=60)
                                                                  GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACG                                   <  SRR4302535.2486564/94‑1 (MQ=60)
                                                                  GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACG                                   <  SRR4302535.3369836/94‑1 (MQ=60)
                                                                  GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACG                                   >  SRR4302535.2433744/1‑94 (MQ=60)
                                                                    GAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTC                                 <  SRR4302535.4042991/94‑1 (MQ=60)
                                                                    GAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTC                                 <  SRR4302535.311813/94‑1 (MQ=60)
                                                                     AAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGATTGTATGACCTGATGGTGACATAAATACGTCA                                >  SRR4302535.3637105/1‑94 (MQ=60)
                                                                      AAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCAT                               <  SRR4302535.3618699/94‑1 (MQ=60)
                                                                      AAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCAT                               <  SRR4302535.3455481/94‑1 (MQ=60)
                                                                      AAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCAT                               >  SRR4302535.62558/1‑94 (MQ=60)
                                                                       AGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATC                              >  SRR4302535.4023491/1‑94 (MQ=60)
                                                                       AGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATC                              <  SRR4302535.3625800/94‑1 (MQ=60)
                                                                       AGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATC                              <  SRR4302535.4509416/94‑1 (MQ=60)
                                                                        GTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCG                             <  SRR4302535.4400094/94‑1 (MQ=60)
                                                                        GTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCG                             >  SRR4302535.3045837/1‑94 (MQ=60)
                                                                          TCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGAT                           <  SRR4302535.4517854/94‑1 (MQ=60)
                                                                          TCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGAT                           <  SRR4302535.2220350/94‑1 (MQ=60)
                                                                          TCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGAT                           <  SRR4302535.2484091/94‑1 (MQ=60)
                                                                           CAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATA                          <  SRR4302535.2137808/94‑1 (MQ=60)
                                                                           CAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATA                          >  SRR4302535.4379759/1‑94 (MQ=60)
                                                                           CAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATA                          >  SRR4302535.4107470/1‑94 (MQ=60)
                                                                           CAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATA                          <  SRR4302535.2255274/94‑1 (MQ=60)
                                                                            AGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAG                         >  SRR4302535.1203734/1‑94 (MQ=60)
                                                                            AGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAG                         <  SRR4302535.683058/94‑1 (MQ=60)
                                                                             GGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGA                        <  SRR4302535.990533/94‑1 (MQ=60)
                                                                               CTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGATG                      <  SRR4302535.3083498/94‑1 (MQ=60)
                                                                               CTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGATG                      >  SRR4302535.608253/1‑94 (MQ=60)
                                                                               CTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGATG                      <  SRR4302535.1495812/94‑1 (MQ=60)
                                                                                TAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGATGA                     <  SRR4302535.2037658/94‑1 (MQ=60)
                                                                                TAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGATGA                     >  SRR4302535.3573229/1‑94 (MQ=60)
                                                                                 AAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGATGAA                    >  SRR4302535.2147542/1‑94 (MQ=60)
                                                                                 AAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGATGAA                    <  SRR4302535.725582/94‑1 (MQ=60)
                                                                                 AAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGATGAA                    <  SRR4302535.2371484/94‑1 (MQ=60)
                                                                                 AAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGATGAA                    >  SRR4302535.1335340/1‑94 (MQ=60)
                                                                                  AGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGATGAAG                   >  SRR4302535.1947173/1‑94 (MQ=60)
                                                                                  AGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGATGAAC                   <  SRR4302535.1306261/94‑1 (MQ=60)
                                                                                    TATCCGGTTGATCTGGGACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGATGAACGT                 >  SRR4302535.3684207/1‑94 (MQ=60)
                                                                                    TATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGATGAACGT                 <  SRR4302535.1124778/94‑1 (MQ=60)
                                                                                     ATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGATGAACGTG                <  SRR4302535.3042875/94‑1 (MQ=60)
                                                                                       CCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGATGAACGTGTA              >  SRR4302535.3637222/1‑94 (MQ=60)
                                                                                       CCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGATGAACGTGTA              >  SRR4302535.3956953/1‑94 (MQ=60)
                                                                                       CCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGATGAACGTGTA              <  SRR4302535.2746862/94‑1 (MQ=60)
                                                                                        CGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGATGAACGTGTAA             <  SRR4302535.466655/94‑1 (MQ=60)
                                                                                         GGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGATGAACGTGTAAT            >  SRR4302535.4629236/1‑94 (MQ=60)
                                                                                          GTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGATGAACGTGTAATA           <  SRR4302535.4714622/94‑1 (MQ=60)
                                                                                           TTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGATGAACGTGTAATAT          <  SRR4302535.1217548/94‑1 (MQ=60)
                                                                                            TGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGATGAACGTGTAATATA         >  SRR4302535.845701/1‑94 (MQ=60)
                                                                                              ATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGATGAACGTGTAATATAAA       >  SRR4302535.3600779/1‑94 (MQ=60)
                                                                                              ATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGATGAACGTGTAATATAAA       >  SRR4302535.1339241/1‑94 (MQ=60)
                                                                                                CTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGATGAACGTGTAATATAAAGA     <  SRR4302535.1549644/94‑1 (MQ=60)
                                                                                                 TGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGATGAACGTGTAATATAAAGAA    <  SRR4302535.190390/94‑1 (MQ=60)
                                                                                                 TGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGATGAACGTGTAATATAAAGAA    >  SRR4302535.3301192/1‑94 (MQ=60)
                                                                                                  GGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGATAGATGAACGTGTAATATAAAGAAA   >  SRR4302535.3276974/1‑94 (MQ=60)
                                                                                                   GAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCAACGATAGATGAACGTGTAATATAAAGAAAA  >  SRR4302535.1801143/1‑94 (MQ=60)
                                                                                                   |                                                                                             
CAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGC‑‑‑‑‑‑GAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAA  >  NC_000913/3934681‑3934867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑